Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ I. N. Tretyakova, E. A. Rogozhin, M. E. Pak [et al.] // Biol. Bull. - 2020. - Vol. 47, Is. 3. - P225-236, DOI 10.1134/S1062359020030097. - Cited References:38. - This work was conducted within the framework of a Budget Project of Sukachev Institute of Forests, Siberian Branch, Russian Academy of Sciences, Federal Research Center Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences"(project no. 0356-2017-0741) and supported by the Russian Foundation for Basic Research, the Government of Krasnoyarsk region, and the Krasnoyarsk Regional Science Foundation within the framework of research project no. 16-44-240509 "The Development of Biotechnology for the Production of Embryogenic Cultures of Siberian Larch Resistant to Fungal Diseases and Pests with the Use of Protective Antimicrobial Peptides in vitro" and no. 18-44-243004 "In vitro Studies of the Effect of Biologically Active Peptides of Plant and Microbial Origin on the Growth and Development of Conifers in Early Ontogenesis." . - ISSN 1062-3590. - ISSN 1608-3059РУБ Biology

Аннотация: The effect of plant antimicrobial peptides on the initiation of callus and embryonal suspensor masses, formation of somatic embryos, and germination of regenerants of Siberian larch has been studied. Protein/peptide extracts isolated fromAmarantus retroflexus(seeds),Nigella sativa(seeds), andElytrigia elongata(spikelets) have been used as objects of plant origin. Peptides have been introduced into the nutrient media at the stage of initiation of embryogenic cultures and somatic embryo germination. The stimulating effect of peptides on the formation of embryogenic cultures of Siberian larch has been found. No other differences in the dynamics of growth in the control and experimental regenerants have been observed. This study is supposed to contribute to enhancing the immunity of the clonal planting stock of Siberian larch.

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Держатели документа:
Russian Acad Sci, Sukachev Inst Forests, Siberian Branch, Div Fed Res Ctr Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia.
Gause Inst New Antibiot, Moscow 119021, Russia.
Tyumen State Univ, Tyumen 625003, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Tretyakova, I. N.; Rogozhin, E. A.; Pak, M. E.; Petukhova, I. A.; Shuklina, A. S.; Pahomova, A. P.; Sadykova, V. S.; Federal Research Center Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences [0356-2017-0741]; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR); Government of Krasnoyarsk region; Krasnoyarsk Regional Science Foundation [16-44-240509, 18-44-243004]

/ F. Gao, C. X. Peng, H. Wang [et al.] // Forests. - 2020. - Vol. 11, Is. 9. - Ст. 912, DOI 10.3390/f11090912. - Cited References:37. - The work was supported by the National Key R&D Program of China (2017YFD0600600), and the Innovation Project of State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University, 2016C01). . - ISSN 1999-4907РУБ Forestry

Аннотация: Korean pine is the dominant species of Korean pine forests. It is an economically valuable species that yields oil, high-quality timber and nuts, and it offers great prospects for further development. Complete regenerated plants of Korean pine were obtained via somatic embryogenesis using megagametophytes as the explant. The seeds of 27 families of Korean pine were collected to induce embryogenic lines. We compared the effects of explant collection time, family and medium components (concentrations of sucrose, plant growth regulators and acid-hydrolyzed casein) on embryogenic lines induction. The effects of plant growth regulators and L-glutamine contents on the proliferation and maturation of embryogenic cell lines were studied, and the germinating ability of different cell lines was evaluated. The embryogenic lines induction percentage of Korean pine reached 33.33%. When 4.52 mu mol center dot L(-1)2,4-D and 2.2 mu mol center dot L(-1)6-BA were added to the medium of embryogenic lines proliferation, the ability of embryo maturation was the best (cell line 001#-100 was 135.71 center dot g(-1)fresh weight). Adding 1-1.5g L-1L-glutamine to the proliferation medium can improve the ability of embryo maturation (cell line 001#-100 was 165.63 center dot g(-1)fresh weight). The germination percentage of the three cell lines tested was significant, and the highest was 66%. We report on successful regeneration and cryopreservation methods for somatic embryos of Korean pine. This technology could be used to propagate the excellent germplasm resources of Korean pine and to establish multi-varietal forestry.

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Держатели документа:
Northeast Forestry Univ, Sch Forestry, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China.
RAS, Forest Siberian Branch, Sukachev Inst, Lab Forest Genet & Breeding,Inst Russian Acad Sci, Krasnoyarsk 660036, Russia.
Timiryazev Russian Acad Sci, Dept Cell Biol, Moscow 127276, Russia.
Timiryazev Russian Acad Sci, Inst Plant Physiol KA, Moscow 127276, Russia.
Lomonosov Moscow State Univ, Fac Biol, Dept Plant Physiol, Moscow 119991, Russia.
State Forestry & Grassland Adm Engn Technol Res C, Harbin 150040, Peoples R China.

Доп.точки доступа:
Gao, Fang; Peng, Chunxue; Wang, Hao; Tretyakova, Iraida Nikolaevna; Nosov, Alexander Mikhaylovich; Shen, Hailong; Yang, Ling; National Key R&D Program of China [2017YFD0600600]; Innovation Project of State Key Laboratory of Tree Genetics and Breeding (Northeast Forestry University) [2016C01]