Труды сотрудников ИЛ им. В.Н. Сукачева СО РАН

/ I. N. Tret'yakova, A. V. Barsukova // Russ. J. Dev. Biol. - 2012. - Vol. 43, Is. 6. - P353-361, DOI 10.1134/S1062360412060082. - Cited References: 32. - This work was supported by the Russian Foundation for Basic Research, project no. 11-04-00281 and Integration Project no. 53 (Genofond of Conifers). . - 9. - ISSN 1062-3604РУБ Developmental Biology

Аннотация: Embryogenic callus formation in different larch species from Siberia (Larix sibirica, L. gmelinii, and L. sukaczewii) was carried out on MSGm medium supplemented with growth regulators (2.4-D and BAP) and followed one and the same scheme: elongation of somatic cells and their asymmetric division with formation of initial and tube cells. The cells of embryo initial underwent sequential divisions and formed embryonic globules which caused the formation of somatic embryos. Somatic embryos became mature and germinated by addition of ABA and PEG into the medium. Long-term proliferating cell lines and regenerant plants were obtained in Sukachev larch and its hybrid with Siberian larch. The success of somatic embryogenesis depended on the genotype of the donor tree.

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Держатели документа:
[Tret'yakova, I. N.
Barsukova, A. V.] Russian Acad Sci, Sukachev Inst Forestry, Siberian Branch, Krasnoyarsk 660036, Russia

Доп.точки доступа:
Tret'yakova, I.N.; Barsukova, A.V.