/ T. G. Volova [et al.]> // Bioresource Technol. - 2013. -
Vol. 146. - P. 215-222,
DOI 10.1016/j.biortech.2013.07.070
. - ISSN 0960-8524
Перевод заглавия: Рост клеток и накопление полигидроксиалканоатов из CO2 и H2 водород-окисляющими бактериями, Cupriavidus eutrophus B-10646
Аннотация: Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D=0.1h-1, on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460h-1, P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, ?-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties. В© 2013 Elsevier Ltd.
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Держатели документа: Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Siberian Fed Univ, Krasnoyarsk, Russia
Russian Acad Sci, Siberian Branch, LV Kirensky Phys Inst, Krasnoyarsk 660036, Russia
Russian Acad Sci, Siberian Branch, Inst Chem & Chem Technol, Krasnoyarsk 660036, Russia;
Доп.точки доступа: Volova, T. G.; Волова, Татьяна Григорьевна; Kiselev, E. G.; Киселев, Евгений Геннадьевич; Shishatskaya, E. I.; Шишацкая, Екатерина Игоревна; Zhila, N. O.; Жила, Наталья Олеговна; Boyandin, A. N.; Бояндин, Анатолий Николаевич; Syrvacheva, D. A.; Vinogradova, O. N.; Kalacheva, G. S.; Калачева, Галина Сергеевна;
Vasiliev, A. D.; Васильев, Александр Дмитриевич; Peterson, I. V.; Петерсон, Иван Викторович