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S54

    Translocation of the products of photosynthesis to roots of pine seedlings
[Text] : научное издание / T. Shiroya et al., // Canadian Journal of Botany. - 1962. - Vol.: 40. - С. 1125-1135 : табл. - Библиогр.: с. 1135
УДК

Кл.слова (ненормированные):
light, darkness, extracts

Аннотация: Pinus strobus or P. resinosa seedlings, 2 or 3 years old, were illuminated in a closed chamber for 1 hour in the presence of C14O2. This was followed by various periods up to 24 hours under different conditions of light and darkness. Then each seedling was divided into its shoot, stem, and roots, and these were extracted separately with 80% ethanol. The extracts were resolved first on resins into sugar, amino acid, and organic acid fractions and then resolved further by paper chromatography. The C14 content of various fractions and the eluted compounds was determined by plating and counting their aliquots. Ethanol-insoluble residue was oxidized and counted as BaC14O3. 8 hours after administration of the C14O2, 91 to 94% of the total C 14 was found in the ethanol-soluble fractions of shoot, stem, or root. In the shoots sugars were found to represent more than 95% of the ethanol-soluble photosynthate, with sucrose forming three-quarters of it. In stem and roots sucrose represented from 75 to 94% of the translocated photosynthate. Raffinose, glucose, and fructose were present in both stem and root.