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Theoretical and experimental decisions in the creation of an artificial ecosystem for human life support in space. / L. V. Kirensky [et al.] // Life sciences and space research. - 1971. - Vol. 9. - P75-80 . - ISSN 0075-9422
Кл.слова (ненормированные):
article -- bacterium -- biotechnology -- Chlorella -- energy metabolism -- human -- instrumentation -- man machine interaction -- metabolism -- microclimate -- space flight -- weightlessness -- Bacteria -- Biotechnology -- Chlorella -- Ecological Systems, Closed -- Energy Metabolism -- Environment, Controlled -- Humans -- Life Support Systems -- Man-Machine Systems -- Space Flight -- Weightlessness
Аннотация: All of man's former space flights were not real ventures into space in the biological sense, as his life was supported with unregenerated earth supplies. The coming stage of space exploration requires man's long existence in the cosmos and on the other planets. This stage of man's activity outside the earth become possible only by creating small man-made ecosystems, permitting the support of his metabolism by the recycling of substances of the terrestrial biosphere. Creation of such systems is a new scientific and technical task. Man-made ecosystems are a new product of man's activity, which have no complete analogy, either in nature, or in technology. Stochastic mechanisms, which stabilize biogeocenosis, cannot be effective in small ecosystems. A technique of parametric control over biosynthesis made it possible to calculate, and put to practice, an ecosystem for man with a cyclic regeneration of the atmosphere, water and, partially, food. The specific bio-technological properties of small man-made ecosystems are being analysed. The possibility of their application for man's excursions into space and for the settlement of other planets is being considered.

Scopus
Держатели документа:
Institute of Physics, Akademgorodok, Krasnoyarsk, USSR. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kirensky, L.V.; Gitelson, I.I.; Terskov, I.A.; Kovrov, B.G.; Lisovsky, G.M.; Okladnikov, Y.N.

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BIOLUMINESCENCE OF A CORAL-REEF [Текст] / I. I. GITELSON, V. S. FILIMONOV // BIOLOGIYA MORYA-MARINE BIOLOGY. - 1980. - Is. 5. - P. 45-51. - Cited References: 7 . - ISSN 0134-3475

РУБ Marine & Freshwater Biology

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
GITELSON, I.I.; FILIMONOV, V.S.

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BACTERIAL BIOLUMINESCENCE AND BIOLUMINESCENT ANALYSIS [Текст] / V. A. KRATASYUK, I. I. GITELSON // Biofizika. - 1982. - Vol. 27, Is. 6. - P. 937-953. - Cited References: 161 . - ISSN 0006-3029

РУБ Biophysics

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
KRATASYUK, V.A.; GITELSON, I.I.

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The role of volatile metabolites in microbial communities of the LSS higher plant link / L. S. Tirranen, I. I. Gitelson // Advances in Space Research. - 2006. - Vol. 38, Is. 6. - P1227-1232, DOI 10.1016/j.asr.2006.02.038 . - ISSN 0273-1177
Кл.слова (ненормированные):
Inhibiting, bactericidal, stimulating effect -- Microbial interaction -- Type of interaction -- Volatile and non-volatile metabolites -- Growth kinetics -- Microbiology -- Microorganisms -- Plants (botany) -- Sensitivity analysis -- Toxic materials -- Inhibiting, bactericidal, stimulating effect -- Microbial interaction -- Type of interaction -- Volatile and non-volatile metabolites -- Metabolites
Аннотация: The paper addresses the possibility of controlling the microbial community composition through metabolites produced by microbes. The comparative analysis of experimental data has shown that volatile metabolites make a much greater contribution to the microbial interactions than nonvolatile ones. It has been found that interaction of microorganisms via the volatiles they release occurs frequently and is typical of a number of microorganisms. Volatile metabolites released by microorganisms produce an inhibitory, sometimes bactericidal, effect on the vital functions of bacteria. The stimulating action occurs 6-8 times less frequently. The range of action on the growth of the test cultures and the range of sensitivity to the effect of volatile metabolites of the study microbes have been found to be individual. Comparative cluster analysis of the ranges of action of 100 study cultures has shown that in the investigated set of microorganism species interaction was performed via a set of volatiles of an inhibiting action (82) and of a stimulating action (52). It has been found that release of volatile metabolites by the studied microorganisms depends upon the culture age, concentrations of components of the nutrient medium, and volatile by-products released by other microorganisms. This production can be increased or decreased by the action of volatile metabolites of other microbes. This is related to strain features and culture age. The prospects of using these regulating metabolites depend on the "range", specificity and safety for other members of the microbial community in insufficient concentrations. Volatiles produced by plants and microorganisms as well as by other components of the system - humans and processing equipment installed inside the closed ecosystem - could influence the formation not only of the microbial community but also of the gas composition of the system's atmosphere, through which they could affect the state of the plants. Specially performed experiments have shown that volatile metabolites of microorganisms can accumulate in their habitat, dissolve in the atmospheric water, and maintain their biological activity for days. It has been determined that volatile metabolites of some microorganisms are toxic for plants. Growth of seedling roots is inhibited more than growth of stems. В© 2006 COSPAR.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Akademgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tirranen, L.S.; Gitelson, I.I.

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Bioluminescence in oceanology. / I. I. Gitelson, L. A. Levin // Journal of bioluminescence and chemiluminescence. - 1989. - Vol. 4, Is. 1. - P555-562 . - ISSN 0884-3996
Кл.слова (ненормированные):
article -- biology -- circadian rhythm -- ecology -- gas -- instrumentation -- luminescence -- oceanography -- Circadian Rhythm -- Ecology -- Gases -- Luminescence -- Marine Biology -- Oceanography
Аннотация: For analytical purposes bioluminescence can be used in three main ways: 1. luminescence measurement of bioluminescent system components isolated in vitro; 2. determination of luminous organisms' reaction to the in vivo test-action; 3. measurement of bioluminescence in marine ecological systems. The majority of the reports of this Symposium are dealing with the first two topics. The aim of our presentation is to draw attention to the third one. The possibilities of bioluminescent analysis are wider than its traditional scheme of applications in the laboratory, when the emitting system is withdrawn from a native source and is placed in a cuvette of the light measuring device. The reverse scheme is also possible, i.e. the device can be introduced into light emitting system such as a marine biocenosis--the community of the sea inhabitants--where we obtain a highly sensitive and rapid means of gaining the information on the vital activity of marine ecosystems, i.e. their spatial structure, rhythms, man's influence upon them, etc. The present communication will consider the possibilities of this form of bioluminescent analysis.

Scopus
Держатели документа:
Institute of Biophysics, USSR Academy of Sciences, Krasnoyarsk. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Levin, L.A.

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Device for the investigation of maximum growth rate of bacteria / I. I. Gitelson, A. M. Kuznetsov, E. K. Rodicheva // Biotechnology Bioengineering Symposium. - 1974. - Vol. 4, Is. II. - P857 . - ISSN 0572-6565
Кл.слова (ненормированные):
bacterial growth -- bacterium -- berol 185 -- control system -- growth -- microorganism -- theoretical study -- vibrionaceae

Scopus
Держатели документа:
Siberian Branch USSR Acad. Sci., Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Kuznetsov, A.M.; Rodicheva, E.K.

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THE QUESTION OF TEMPORAL ORGANIZATION OF BACTERIAL LUMINESCENCE [Текст] / S. I. BARTSEV, I. I. GITELSON // STUDIA BIOPHYSICA. - 1985. - Vol. 105, Is. 3. - P. 149-156. - Cited References: 17 . - ISSN 0081-6337

РУБ Biophysics

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
BARTSEV, S.I.; GITELSON, I.I.

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Self-restoration as fundamental property of CES providing their sustainability [Text] / I. I. Gitelson, A. G. Degermendzhy, E. K. Rodicheva ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1641-1648, DOI 10.1016/S0273-1177(03)00111-X. - Cited References: 8 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
LIFE-SUPPORT
Аннотация: Sustainability is one of the most important criteria in the creation and evaluation of human life support systems intended for use during long space flights. The common feature of biological and physicochemical life support systems is that basically they are both catalytic. But there are two fundamental properties distinguishing biological systems: 1) they are auto-catalytic: their catalysts - enzymes of protein nature - are continuously reproduced when the system functions; 2) the program of every process performed by enzymes and the program of their reproduction are inherent in the biological system itself - in the totality of genomes of the species involved in the functioning of the ecosystem. Actually, one cell with the genome capable of the phenotypic realization is enough for the self-restoration of the function performed by the cells of this species in the ecosystem. The continuous microalgal culture of Chlorella vulgaris was taken to investigate quantitatively the process of self-restoration in unicellular algae population. Based on the data obtained, we proposed a mathematical model of the restoration process in a cell population that has suffered an acute radiation damage. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Krasnoyarsk State Univ, Inst Biophys, SB RAS, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Degermendzhy, A.G.; Rodicheva, E.K.; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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Designing a reusable system based on nanodiamonds for biochemical determination of urea / N. O. Ronzhin [et al.] // Doklad. Biochem. Biophys. - 2015. - Vol. 465, Is. 1. - P428-431, DOI 10.1134/S1607672915060216 . - ISSN 1607-6729
Аннотация: A reusable system including urease covalently bound to the surface of modified nanodiamonds (MNDs) has been developed for the multiple determination of urea. The immobilized enzyme exhibits functional activity and catalyzes the hydrolysis of urea to yield ammonia. The presence of ammonia is confirmed by the formation of a colored product after the addition of chemical reagents. It was shown that the MNDs-urease complex can function in a wide range of temperatures and pH as well as in deionized water. The complex provides a linear yield of the product at low analyte concentrations and allows the multiple determination of urea in vitro. © 2015, Pleiades Publishing, Ltd.

Scopus,
WOS
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Ronzhin, N. O.; Baron, A. V.; Bondar, V. S.; Gitelson, I. I.

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10.

577.151.45
П 65


    Почему у биолюминесцентного опёнка осеннего (Armillaria mellea) светится мицелий, но не светится плодовое тело? [Текст] : статья / К. В. Пуртов [и др.] // Доклады Академии наук. - 2017. - Т. 474, № 4. - С. 510-512, DOI 10.7868/S0869565217040247 . - ISSN 0869-5652
   Перевод заглавия: Why Does Bioluminescent Fungus Armillaria mellea Have Luminous Mycelium but Nonluminous Fruiting Body?

УДК

577.151.45

Аннотация: С помощью определения компонентов-участников биолюминесцентного процесса в светящихся и несветящихся органах опёнка осеннего выяснили причины неполного свечения Armillaria mellea. Полный набор ферментов и субстратов, необходимых для биолюминесценции, образуется только в мицелии, причём исключительно в условиях свободного доступа кислорода. В плодовых телах синтез предшественника люциферина (гиспидина) и 3-гидроксигиспидингидроксилазы блокирован, вследствие этого и не происходило образование люциферина - ключевого компонента биолюминесцентной системы грибов. Именно поэтому плодовое тело опёнка осеннего не светится, несмотря на наличие люциферазы - фермента, катализирующего окисление люциферина с испусканием кванта света.

РИНЦ
Держатели документа:
Федеральный исследовательский центр “Красноярский научный центр Сибирского отделения Российская Академия наук

Доп.точки доступа:
Пуртов, К.В.; Purtov K.V.; Петушков, В.Н.; Petushkov V.N.; Родионова, Н.С.; Rodionova N.S.; Гительзон, И.И.; Gitelson I.I.

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11.

BIOLUMINESCENCE IN OCEANOLOGY [Text] / I. I. GITELSON, L. A. LEVIN // J. Biolumin. Chemilumin. - 1988. - Vol. 2, Is. 4. - P. 206-206. - Cited References: 0 . - ISSN 0884-3996

РУБ Biochemistry & Molecular Biology

WOS
Держатели документа:
ACAD SCI USSR,INST BIOPHYS,KRASNOYARSK 660036,USSR
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
GITELSON, I.I.; LEVIN, L.A.

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12.

BIOLUMINESCENCE IN OCEANOLOGY [Text] / I. I. GITELSON, L. A. LEVIN // J. Biolumin. Chemilumin. - 1989. - Vol. 4, Is. 1. - P. 555-562, DOI 10.1002/bio.1170040173. - Cited References: 19 . - ISSN 0884-3996

РУБ Biochemistry & Molecular Biology

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
GITELSON, I.I.; LEVIN, L.A.

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13.

UNDERWAY MEASUREMENT OF BIOMINESCENT FIELD DURING BIOLOGICAL EXPEDITION [Текст] / I. I. GITELSON, R. N. UTYUSHEV, A. V. SHELEGOV // Okeanologiya. - 1991. - Vol. 31, Is. 4. - P. 631-637. - Cited References: 5 . - ISSN 0030-1574

РУБ Oceanography

Аннотация: Data on surface bioluminescence intensity measurements during the 15-th cruise of R/V Vityaz, January to March 1988, in the Indian Ocean, the Mediterranean and Black Seas are presented. High luminescence intensity is found in the Arabian and Black Seas and the Dardanelles. Recommendations on underway measurements of biological and hydrophysical parameters are given.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
GITELSON, I.I.; UTYUSHEV, R.N.; SHELEGOV, A.V.

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14.

Affine magnetic sorbents supported on coal ash microspheres for recombinant protein isolation / L. A. Frank [et al.] // Applied Biochemistry and Microbiology. - 2009. - Vol. 45, Is. 2. - P215-220, DOI 10.1134/S0003683809020173 . - ISSN 0003-6838
Кл.слова (ненормированные):
Clytia gregaria -- Obelia longissima
Аннотация: The results of the development and utilization of an affine magnetic sorbent with Ni2+ ions immobilized on coal ash microspheres are reported. The applicability of the material in the isolation of Histag proteins is demonstrated by examples of the recombinant green fluorescent protein from Clytia gregaria and the Ca2+ regulated photoprotein obelin from Obelia longissima. The specific sorption capacity of the sorbent was 2-7 mg/cm3 for medium-size proteins (20-30 kDa). The particles are suitable for chromatography with the presence of chaotropic agents and EDTA. They are easy to manipulate as isolation of a target protein takes 30-35 min. On the one hand, the elevated affinity of the sorbent to proteins rich in native histidines may result in a high degree of irreversible sorption; on the other hand, it allows isolation of such proteins without the introduction of artificial polyhistidine fragments. В© 2009 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Institute of Chemistry and Chemical Technology, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660049, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Frank, L.A.; Borisova, V.V.; Vereshchagina, T.A.; Fomenko, E.V.; Anshits, A.G.; Gitelson, I.I.

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15.

Investigation of application of PHA coating to enhance biocompatibility of vascular stents / A. V. Protopopov [et al.] // Doklady Biological Sciences. - 2005. - Vol. 401, Is. 1-6. - P85-87, DOI 10.1007/s10630-005-0051-8 . - ISSN 0012-4966
Кл.слова (ненормированные):
biomaterial -- polymer -- animal -- article -- blood vessel transplantation -- chemistry -- dog -- stent -- Animals -- Blood Vessel Prosthesis Implantation -- Coated Materials, Biocompatible -- Dogs -- Polymers -- Stents

Scopus
Держатели документа:
Krasnoyarsk Regional Clinical Hospital No. 1, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk Regional Postmortem Examination Center, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Protopopov, A.V.; Kochkina, T.A.; Konstantinov, E.P.; Shishatskaya, E.I.; Efremov, S.N.; Volova, T.G.; Gitelson, I.I.

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16.

In vivo toxicological evaluation of polyhydroxyalkanoates. / E. I. Shishatskaya, T. G. Volova, I. I. Gitelson // Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - P109-111 . - ISSN 0012-4966
Кл.слова (ненормированные):
alkane -- animal -- animal behavior -- article -- cytology -- drug effect -- erythrocyte sedimentation rate -- female -- heart -- kidney -- liver -- lymphoid tissue -- male -- rat -- spleen -- Wistar rat -- Alkanes -- Animals -- Behavior, Animal -- Blood Sedimentation -- Female -- Heart -- Kidney -- Liver -- Lymphoid Tissue -- Male -- Rats -- Rats, Wistar -- Spleen

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gitelson, I.I.

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17.

On the involvement of macrophages and phosphomonoesterases in the tissue response to implantation of polyhydroxyalkanoates. / E. I. Shishatskaya, T. G. Volova, I. I. Gitelson // Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - P116-119 . - ISSN 0012-4966
Кл.слова (ненормированные):
alkane -- phosphatase -- animal -- article -- comparative study -- foreign body reaction -- macrophage -- metabolism -- physiology -- rat -- Wistar rat -- Alkanes -- Animals -- Foreign-Body Reaction -- Macrophages -- Phosphoric Monoester Hydrolases -- Rats -- Rats, Wistar

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Gitelson, I.I.

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18.

Self-restoration as fundamental property of CES providing their sustainability / I. I. Gitelson, A. G. Degermendzhy, E. K. Rodicheva // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1641-1648, DOI 10.1016/S0273-1177(03)00111-X . - ISSN 0273-1177
Кл.слова (ненормированные):
Biocatalysts -- Ecosystems -- Genes -- Life support systems (spacecraft) -- Radiation damage -- Cell populations -- Space flight -- space shuttle -- article -- biological model -- biomass -- cell division -- Chlorella -- cytology -- growth, development and aging -- microclimate -- radiation exposure -- radiation response -- ultraviolet radiation -- Biomass -- Cell Division -- Chlorella -- Dose-Response Relationship, Radiation -- Ecological Systems, Closed -- Life Support Systems -- Models, Biological -- Ultraviolet Rays
Аннотация: Sustainability is one of the most important criteria and evaluation of human life support systems intended for use during long space flights. The common feature of biological and physicochemical life support systems is that basically they are both catalytic. But there are two fundamental properties distinguishing biological systems: 1) they are auto-catalytic: their catalysts - enzymes of protein nature - are continuously reproduced when the system functions; 2) the program of every process performed by enzymes and the program of their reproduction are inherent in the biological system itself - in the totality of genomes of the species involved in the functioning of the ecosystem. Actually, one cell with the genome capable of the phenotypic realization is enough for the self-restoration of the function performed by the cells of this species in the ecosystem. The continuous microalgal culture of Chlorella vulgaris was taken to investigate quantitatively the process of self-restoration in unicellular algae population. Based on the data obtained, we proposed a mathematical model of the restoration process in a cell population that has suffered an acute radiation damage. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Degermendzhy, A.G.; Rodicheva, E.K.

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19.

Microbial degradation of polyhydroxyalkanoates in tropical soils / A. N. Boyandin [et al.] // International Biodeterioration and Biodegradation. - 2013. - Vol. 83. - P77-84, DOI 10.1016/j.ibiod.2013.04.014 . - ISSN 0964-8305
Кл.слова (ненормированные):
Biopolymer properties -- Biopolymers -- PHA degrading microorganisms -- Polyhydroxyalkanoates (PHAs) -- Soil biodegradation -- 3-hydroxybutyric acid -- Degree of crystallinity -- Hydroxyalkanoic acids -- Microbial characteristics -- Microbial degradation -- Polyhydroxyalkanoates -- Polymer molecular mass -- Soil biodegradations -- Bacteriology -- Biomolecules -- Biopolymers -- Microorganisms -- Soils -- Tropics -- Biodegradation -- biodegradation -- chemical composition -- microbial activity -- microbial community -- molecular analysis -- organic compound -- polymer -- soil degradation -- soil microorganism -- tropical soil
Аннотация: The integrated study addressing biodegradation of microbial linear polyesters of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) in tropical conditions by microbial communities of Vietnamese soils was performed in locations close to Hanoi and Nha Trang, which differed in their weather conditions and microbial communities. It shows that PHA degradation in tropical soils is influenced by polymer chemical composition, specimen shape, and microbial characteristics. The homopolymer of 3-hydroxybutyric acid is degraded at higher rates than the copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids. The average rates of mass loss were 0.04-0.33% per day for films and 0.02-0.18% for compact pellets. PHA degradation was accompanied by a decrease in the polymer molecular mass and, usually, an increase in the degree of crystallinity, suggesting preferential degradation of the amorphous phase. Under the study conditions, representatives of the bacterial genera Burkholderia, Bacillus, Cupriavidus, Mycobacterium, and Nocardiopsis and such micromycetes as Acremonium, Gongronella, Paecilomyces, and Penicillium, Trichoderma have been identified as major PHA degraders. В© 2013 Elsevier Ltd.

Scopus
Держатели документа:
Institute of Biophysics of Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russian Federation
The Joint Russian-Vietnam Tropical Research and Test Center, Hanoi, Viet Nam
Inst. of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Prudnikova, S.V.; Karpov, V.A.; Ivonin, V.N.; D?, N.L.; Nguy?n, T.H.; Le, T.M.H.; Filichev, N.L.; Levin, A.L.; Filipenko, M.L.; Volova, T.G.; Gitelson, I.I.

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20.

Biodegradation of polyhydroxyalkanoates (PHAs) in tropical coastal waters and identification of PHA-degrading bacteria / T. G. Volova [et al.] // Polymer Degradation and Stability. - 2010. - Vol. 95, Is. 12. - P2350-2359, DOI 10.1016/j.polymdegradstab.2010.08.023 . - ISSN 0141-3910
Кл.слова (ненормированные):
Biopolymers in marine environment -- Degradation of polymers -- PHA properties -- PHA-degrading microorganisms -- Polyhydroxyalkanoates, PHA -- 16S rRNA -- 3-hydroxybutyric acid -- Amorphous phase -- Bacillus sp -- Biodegradation rate -- Chemical compositions -- Coastal waters -- Degradation rate -- Degrading bacteria -- Degree of crystallinity -- Enterobacter -- Hydroxyvalerate -- Marine environment -- PHA properties -- PHA-degrading microorganisms -- Polydispersity indices -- Polyhydroxyalkanoates -- Polymer chains -- Preparation technique -- Sole carbon source -- South China Sea -- Viet Nam -- Acids -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Biomolecules -- Biopolymers -- Microorganisms -- Organic polymers -- Polydispersity -- Polymer films -- Polymers -- RNA -- Seawater -- Degradation
Аннотация: Biodegradability patterns of two PHAs: a polymer of 3-hydroxybutyric acid (3-PHB) and a copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids (3-PHB/3-PHV) containing 11 mol% of hydroxyvalerate, were studied in the tropical marine environment, in the South China Sea (Nha Trang, Vietnam). No significant differences have been observed between degradation rates of 3-PHB and 3-PHB/3-PHV specimens; it has been found that under study conditions, biodegradation is rather influenced by the shape of the polymer item and the preparation technique than by the chemical composition of the polymer. Biodegradation rates of polymer films in seawater have been found to be higher than those of compacted pellets. As 3-PHB and 3-PHB/3-PHV are degraded and the specimens lose their mass, molecular weight of both polymers is decreased, i.e. polymer chains get destroyed. The polydispersity index of the PHAs grows significantly. However, the degree of crystallinity of both PHAs remains unchanged, i.e. the amorphous phase and the crystalline one are equally disintegrated. PHA-degrading microorganisms were isolated using the clear-zone technique, by inoculating the isolates onto mineral agar that contained PHA as sole carbon source. Based on the 16S rRNA analysis, the PHA-degrading strains were identified as Enterobacter sp. (four strains), Bacillus sp. and Gracilibacillus sp.В© 2010 Elsevier Ltd. All rights reserved.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, 79 Svobodny av., Krasnoyarsk 660041, Russian Federation
L.V. Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
A.N. Severtsov Institute of Ecology and Evolution RAS, 33 Leninskij Prospect, Moscow 119071, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 8 Lavrentiev Ave., Novosibirsk 630090, Russian Federation
Joint Russian-Vietnam Tropical Research and Test Centre, Coastal Branch, 30 Nguyen Thien Thuat, Nha Trang, Viet Nam : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Boyandin, A.N.; Vasiliev, A.D.; Karpov, V.A.; Prudnikova, S.V.; Mishukova, O.V.; Boyarskikh, U.A.; Filipenko, M.L.; Rudnev, V.P.; Ba Xuan, B.; Vit Dung, V.; Gitelson, I.I.

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