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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondareva L.G., Bolsunovskii A.Ya.
Заглавие : Speciation of artificial radionuclides 60Co, 137Cs, 152Eu, and 241Am in bottom sediments of the Yenisei river
Место публикации : Radiochemistry. - 2008. - Vol. 50, Is. 5. - С. 547-552. - ISSN 10663622 (ISSN) , DOI 10.1134/S1066362208050196
Аннотация: The speciation of artificial radionuclides 60Co, 137Cs, 152Eu, and 241Am in surface layers of bottom sediments of the Yenisei river, collected within the near zone of impact of the Mining and Chemical Combine (Rosatom), was studied. In these samples the radionuclides 60Co, 152Eu, and 241Am are mainly associated with fractions of humic and fulvic acids. The fraction of 152Eu associated with nonsilicate iron is approximately 92%. The migration capability of radionuclides collected near the Atamanovo settlement decreases in the order 152Eu > 241Am > 60Co > 137Cs. For samples collected near Bol'shoi Balchug settlement, this order is as follows: 152Eu ? 241Am > 60Co > 137Cs. The presence of radionuclide-bearing micro-particles in bottom sediments considerably complicates the distribution of radionuclides, in particular, of 241Am and 137Cs, among migration forms. В© 2008 MAIK Nauka.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondareva L.G., Kalyakina O.P., Bolsunovskii A.Ya.
Заглавие : Effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system as studied by dual-column ion chromatography and ?-ray spectrometry
Место публикации : Journal of Analytical Chemistry. - 2006. - Vol. 61, Is. 4. - С. 354-358. - ISSN 10619348 (ISSN) , DOI 10.1134/S1061934806040101
Ключевые слова (''Своб.индексиров.''): absorption--chromatographic analysis--complexation--gamma ray spectrometers--radioisotopes--?-ray spectrometry--absorption-release processes--dual-column ion chromatography--humic acid--organic acids
Аннотация: The effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system was studied by dual-column ion chromatography and ?-ray spectrometry. With the use of ion chromatography, it was found that processes related to the absorption of SO 42- and Cl- anions by a solid phase with the release of NO 3- , PO 43- , and F- to a liquid phase competed in the test systems as the concentration of water-soluble organic carbon (WSOC) was increased. Only the test anions were released in the systems without the introduction of an additional amount of WSOC as humic acid. With the use of ?-ray spectrometry, it was found that the release of 60Co, 152Eu, and 241Am radionuclides to the liquid phase in the systems with added humic acid began much earlier than in the system without the addition of humic acid. In this case, the amount of released radionuclides was greater than the amount of radioisotopes released in the system without the addition of humic acid: ?25% 241Am, ?3% 152Eu, and ?0.8% 60Co in the system with added humic acid or 0.8% 152Eu and <0.1% 60Co in the system without the addition of humic acid. The 241Am radionuclide was not determined in the system without the addition of humic acid. An increase in the concentration of WSOC in the experimental system of bottom sediments-Yenisei river water initiated the release of 60Co, 152Eu, and 241Am anthropogenic radionuclides from bottom sediments because of the formation of soluble complexes capable of migration. An increase in the concentration of WSOC had almost no effect on the release of 40K and 137Cs radionuclides. В© Pleiades Publishing, Inc., 2006.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N.O., Kalacheva G.S., Volova T.G., Degermendzhi A.G.
Заглавие : Structure of hydrocarbons synthesized by the alga Botryococcus isolated from Lake Shira.
Место публикации : Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections / translated from Russian. - 2001. - Vol. 378. - С. 265-269. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): fatty acid--hydrocarbon--sea water--article--chemical structure--chemistry--classification--green alga--isolation and purification--metabolism--microbiology--russian federation--species difference--algae, green--fatty acids--hydrocarbons--molecular structure--seawater--siberia--species specificity
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Prudnikova S.V., Karpov V.A., Ivonin V.N., D? N.L., Nguy?n T.H., Le T.M.H., Filichev N.L., Levin A.L., Filipenko M.L., Volova T.G., Gitelson I.I.
Заглавие : Microbial degradation of polyhydroxyalkanoates in tropical soils
Место публикации : International Biodeterioration and Biodegradation. - 2013. - Vol. 83. - С. 77-84. - ISSN 09648305 (ISSN) , DOI 10.1016/j.ibiod.2013.04.014
Ключевые слова (''Своб.индексиров.''): biopolymer properties--biopolymers--pha degrading microorganisms--polyhydroxyalkanoates (phas)--soil biodegradation--3-hydroxybutyric acid--degree of crystallinity--hydroxyalkanoic acids--microbial characteristics--microbial degradation--polyhydroxyalkanoates--polymer molecular mass--soil biodegradations--bacteriology--biomolecules--biopolymers--microorganisms--soils--tropics--biodegradation--biodegradation--chemical composition--microbial activity--microbial community--molecular analysis--organic compound--polymer--soil degradation--soil microorganism--tropical soil
Аннотация: The integrated study addressing biodegradation of microbial linear polyesters of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) in tropical conditions by microbial communities of Vietnamese soils was performed in locations close to Hanoi and Nha Trang, which differed in their weather conditions and microbial communities. It shows that PHA degradation in tropical soils is influenced by polymer chemical composition, specimen shape, and microbial characteristics. The homopolymer of 3-hydroxybutyric acid is degraded at higher rates than the copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids. The average rates of mass loss were 0.04-0.33% per day for films and 0.02-0.18% for compact pellets. PHA degradation was accompanied by a decrease in the polymer molecular mass and, usually, an increase in the degree of crystallinity, suggesting preferential degradation of the amorphous phase. Under the study conditions, representatives of the bacterial genera Burkholderia, Bacillus, Cupriavidus, Mycobacterium, and Nocardiopsis and such micromycetes as Acremonium, Gongronella, Paecilomyces, and Penicillium, Trichoderma have been identified as major PHA degraders. В© 2013 Elsevier Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Murueva A.V., Shishatskaya E.I., Kuzmina A.M., Volova T.G., Sinskey A.J.
Заглавие : Microparticles prepared from biodegradable polyhydroxyalkanoates as matrix for encapsulation of cytostatic drug
Место публикации : Journal of Materials Science: Materials in Medicine. - 2013. - Vol. 24, Is. 8. - С. 1905-1915. - ISSN 09574530 (ISSN) , DOI 10.1007/s10856-013-4941-2
Ключевые слова (''Своб.индексиров.''): 3-hydroxybutyric acid--average diameter--cell attachments--chemical compositions--mass concentration--mouse-fibroblasts--polyhydroxyalkanoates--solvent evaporation techniques--biocompatibility--cell culture--cells--loading--3 hydroxybutyric acid--3 hydroxyhexanoic acid--4 hydroxybutyric acid--4',6 diamidino 2 phenylindole--dna--doxorubicin--nanoparticle--polyhydroxyalkanoic acid--polymer--polystyrene--solvent--unclassified drug--animal cell--article--biocompatibility--biodegradability--cell adhesion--cell proliferation--cell strain 3t3--cell viability--chemical composition--chemical structure--controlled study--cytotoxicity--drug efficacy--drug release--electrophoretic mobility--encapsulation--evaporation--fibroblast--in vitro study--nonhuman--particle size--priority journal--stain--study--surface charge--zeta potential
Аннотация: Microparticles made from degradable polyhydroxyalkanoates of different chemical compositions a homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric and 3-hydroxyvaleric acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoic acids (P3HB/3HHx) were prepared using the solvent evaporation technique, from double emulsions. The study addresses the influence of the chemical compositions on the size and ?-potential of microparticles. P3HB microparticles loaded with doxorubicin have been prepared and investigated. Their average diameter and ?-potential have been found to be dependent upon the level of loading (1, 5, and 10 % of the polymer mass). Investigation of the in vitro drug release behavior showed that the total drug released from the microparticle into the medium increased with mass concentration of the drug. In this study mouse fibroblast NIH 3T3 cells were cultivated on PHA microparticles, and results of using fluorescent DAPI DNA stain, and MTT assay showed that microparticles prepared from PHAs of different chemical compositions did not exhibit cytotoxicity to cells cultured on them and proved to be highly biocompatible. Cell attachment and proliferation on PHA microparticles were similar to those on polystyrene. The cytostatic drug encapsulated in P3HB/3HV microparticles has been proven to be effective against HeLa tumor cells. В© 2013 Springer Science+Business Media New York.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Nikolaeva E.D., Goreva A.V., Brigham C.J., Volova T.G., Sinskey A.J.
Заглавие : An in vivo study of pha matrices of different chemical composition: Tissue reaction and biodegradation
Место публикации : Cellular Transplantation and Tissue Engineering. - 2012. - Vol. 7, Is. 1. - С. 73-80. - ISSN 1815445X (ISSN)
Ключевые слова (''Своб.индексиров.''): degradable polymer matrices--degradation--phas--polyhydroxyalkanoates--subcutaneous implantation--tissue reaction
Аннотация: The study addresses consequences of subcutaneous implantation of film matrices prepared from different PHAs to laboratory animals. No negative effects of subcutaneous implantation of PHA matrices on physiological and biochemical characteristics of the animals were determined. Independently of the matrices composition and duration of the contact with the internal environment of the organism we did not observe any deviations in the behavior of animals, their growth and development, as well as blood functions. Response of the tissues to PHA matrices was comparable with the response to polylactide, but substantially less expressed at the earlier time periods after implantation. Tissues response to implantation of PHA of all types is characterized by short-term (up to 2 weeks) post-traumatic inflammation with formation of fibrous capsules by 30th-60th days with the thickness less than 100 microns, which get thinner down to 40-60 microns by 180th day as the result of involution. No differences in response of tissues and the whole organism were observed for the matrices produced from the homopolymer of 3-hydroxybutyric acid (P3HB), copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric acid and 3-hydroxyvalerianic acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoate acids (P3HB/3HH). Macrophages and foreign-body giant cells actively participate in the response of the tissues to PHAs. In the studied conditions matrices from the copolymers containing 3-hydroxyhexanoate and 4 hydroxybutyrate were determined as more actively degraded PHA. The next less degraded matrices were matrices from the copolymer of P3HB/3HV and the most resistant were P3HB matrices. The slower degradation of PHA matrices was accompanied by delayed development of giantcells response. The studied PHA matrices can be placed in the following range by their degradation: P3HB/3HH - P3HB/4HB - P3HB/HV - P3HB.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nikolaeva E.D., Shishatskaya E.I., Mochalov K.E., Volova T.G., Sinsky A.J.
Заглавие : Comparative investigation of polyhydroxyalkanoate scaffolds with various chemical compositions
Место публикации : Cellular Transplantation and Tissue Engineering. - 2011. - Vol. 6, Is. 4. - С. 54-63. - ISSN 1815445X (ISSN)
Ключевые слова (''Своб.индексиров.''): biopolymers--scaffolds--tissue engineering
Аннотация: The authors have constructed and characterized a series of membranes based on resorbable polyhydroxyalkanoates of different compositions. Five PHA types have been studied: a homopolymer of 3-hydroxybutyric acid, copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids, 3-hydroxybutyric and 3-hydroxyvaleric acids, 3-hydroxybutyric and 3-hydroxyhexanoic acids. Scanning electron microscopy and atomicforce microscopy were used to examine the microstructure of membrane surfaces, showing that membranes based on the copolymer of 3-hydroxybutyrate and 3-hydroxyhexanoate had the roughest surface, while membranes based on the copolymer of 3-hydroxybutyrate and 3-hydroxyvalerate had the smoothest surface. The contact angle for water in air was smaller and hydrophilic properties better in the copolymer membranes than in the membranes based on the high-crystallinity homopolymer of 3-hydroxybutyric acid. The culture of mouse fibroblast cell line NIH 3,3 was used to test PHAbased membranes; results of fluorescent probes of DNA DAPI and the MTT assay show that membranes based on studied PHAs are not cytotoxic on direct contact with cells and are highly biocompatible; their adhesive properties and ability to maintain fibroblast proliferation are similar to those of polystyrene and better than those of polylactic acid membranes.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N.O., Kalacheva G.S., Volova T.G.
Заглавие : Effect of salinity on the biochemical composition of the alga Botryococcus braunii Kutz IPPAS H-252
Место публикации : Journal of Applied Phycology. - 2011. - Vol. 23, Is. 1. - С. 47-52. - ISSN 09218971 (ISSN) , DOI 10.1007/s10811-010-9532-8
Ключевые слова (''Своб.индексиров.''): botryococcus--fatty acid composition--lipid content--salinity--algae--botryococcus--botryococcus braunii
Аннотация: The effect of 0.3 and 0.7 M NaCl on biomass yield, total nitrogen content, intracellular lipid content, and fatty acid profile of the lipids of the alga Botryococcus braunii IPPAS H-252 in different phases of the culture cycle was studied. The presence of sodium chloride in the medium inhibited the growth of algal cells for the first 3 days of the experiment, causing a decrease in total nitrogen, enhanced synthesis of triacylglycerols, and considerable changes in the lipid fatty acid profile: decreases in polyenoic acid contents (from 68.34% to 29.38% and 12.8%) and proportions of long-chain saturated acids (from 0.53% to 5.3% and 14.13% of the total fatty acids) at 0.3 M NaCl and 0.7 M NaCl, respectively. In later phases of the culture, at 0.3 M NaCl, the content of polyenoic acids rose to the values characteristic of the active growth phase of this alga. At 0.7 M NaCl, the proportion of polyenoic acids grew less significantly, but biomass concentration and total nitrogen increased, similarly to the experiment with 0.3 M NaCl. В© 2010 Springer Science+Business Media B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Boyandin A.N., Vasiliev A.D., Karpov V.A., Prudnikova S.V., Mishukova O.V., Boyarskikh U.A., Filipenko M.L., Rudnev V.P., Ba Xuan B., Vit Dung V., Gitelson I.I.
Заглавие : Biodegradation of polyhydroxyalkanoates (PHAs) in tropical coastal waters and identification of PHA-degrading bacteria
Место публикации : Polymer Degradation and Stability. - 2010. - Vol. 95, Is. 12. - С. 2350-2359. - ISSN 01413910 (ISSN) , DOI 10.1016/j.polymdegradstab.2010.08.023
Ключевые слова (''Своб.индексиров.''): biopolymers in marine environment--degradation of polymers--pha properties--pha-degrading microorganisms--polyhydroxyalkanoates, pha--16s rrna--3-hydroxybutyric acid--amorphous phase--bacillus sp--biodegradation rate--chemical compositions--coastal waters--degradation rate--degrading bacteria--degree of crystallinity--enterobacter--hydroxyvalerate--marine environment--pha properties--pha-degrading microorganisms--polydispersity indices--polyhydroxyalkanoates--polymer chains--preparation technique--sole carbon source--south china sea--viet nam--acids--bacteriology--biodegradable polymers--biodegradation--biomolecules--biopolymers--microorganisms--organic polymers--polydispersity--polymer films--polymers--rna--seawater--degradation
Аннотация: Biodegradability patterns of two PHAs: a polymer of 3-hydroxybutyric acid (3-PHB) and a copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids (3-PHB/3-PHV) containing 11 mol% of hydroxyvalerate, were studied in the tropical marine environment, in the South China Sea (Nha Trang, Vietnam). No significant differences have been observed between degradation rates of 3-PHB and 3-PHB/3-PHV specimens; it has been found that under study conditions, biodegradation is rather influenced by the shape of the polymer item and the preparation technique than by the chemical composition of the polymer. Biodegradation rates of polymer films in seawater have been found to be higher than those of compacted pellets. As 3-PHB and 3-PHB/3-PHV are degraded and the specimens lose their mass, molecular weight of both polymers is decreased, i.e. polymer chains get destroyed. The polydispersity index of the PHAs grows significantly. However, the degree of crystallinity of both PHAs remains unchanged, i.e. the amorphous phase and the crystalline one are equally disintegrated. PHA-degrading microorganisms were isolated using the clear-zone technique, by inoculating the isolates onto mineral agar that contained PHA as sole carbon source. Based on the 16S rRNA analysis, the PHA-degrading strains were identified as Enterobacter sp. (four strains), Bacillus sp. and Gracilibacillus sp.В© 2010 Elsevier Ltd. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Steinbuchel A.
Заглавие : Biosynthesis of multi-component polyhydroxyalkanoates by the bacterium Wautersia eutropha
Место публикации : Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - С. 1-7. - ISSN 10221360 (ISSN) , DOI 10.1002/masy.200850901
Ключевые слова (''Своб.индексиров.''): autotrophic and mixotrophic growth--multi-component polyhydroxyalkanoates--wautersia eutropha--bacteriology--biochemical engineering--biochemistry--biopolymers--biotechnology--carboxylic acids--fatty acids--ketones--monomers--polymers--autotrophic and mixotrophic growth--carbon chains--carbon nutritions--even numbers--growth conditions--hydroxybutyrate--hydroxyvalerate--mixotrophic--multi-component polyhydroxyalkanoates--odd numbers--physico-chemical properties--polyhydroxyalkanoates--synthesis of--wautersia eutropha--wild types--acids
Аннотация: The study addresses the effect of different conditions of carbon nutrition on synthesis of polyhydroxyalkanoates by the bacterium Wautersia eutropha. In experiments with two wild type strains (H16 and 65786), it has been first found that under mixotrophic growth conditions - Co2 + co-substrate (alkanoic acids) - bacteria can synthesize multi-component PHAs, consisting of short- and medium-chainlength monomers with carbon chains containing 4 to 8 atoms. It has been shown that PHA composition is determined by the type of the co-substrate. Fatty acids with odd number of carbons induce bacteria to synthesize four- and five-component PHAs with hydroxybutyrate, hydroxyvalerate as major monomers and hydroxyhexanoate, hydroxyheptanoate and hydroxyoctanoate as minor, occasionally occurring, ones. Fatty acids with even number of carbons induce synthesis of not only their respective monomers (hydroxyhexanoate and hydroxyoctanoate) but also hydroxyvalerate, making possible synthesis of four-component PHAs, containing hydroxybutyrate and hydroxyhexanoate as major components (up to 18 mol%). A family of short- and medium-chain-length four- and five-component PHAs were synthesized and their physicochemical properties examined. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A., Kalacheva G.S., Medvedeva S., Rodicheva E., Volova T.G.
Заглавие : Luminous bacteria as producers of polyhydroxyalkanoates
Место публикации : Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - С. 17-22. - ISSN 10221360 (ISSN) , DOI 10.1002/masy.200850904
Ключевые слова (''Своб.индексиров.''): luminous bacteria--polyhydroxyalkanoates--polyhydroxybutyrate--abs resins--acids--bacteriology--batch cell culture--biological materials--biomass--biopolymers--biotechnology--cell culture--esters--hydrocarbons--organic compounds--polymers--renewable energy resources--supramolecular chemistry--batch cultures--dry cells--luminous bacteria--micro-organisms--photobacterium leiognathi--photobacterium phosphoreum--polyhydroxyalkanoates--polyhydroxybutyrate--polymer yields--vibrio fischeri--bioluminescence
Аннотация: The study addresses the ability of luminous bacteria of different taxa (Photobacterium leiognathi, Photobacterium phosphoreum, Vibrio harveyi, Vibrio fischeri) to synthesize polyesters of hydrocarbon acids (polyhydroxyalkanoates, PHAs) as storage macromolecules. The screened strains widely varied in their PHA productivity. Conditions for attaining high polymer yields (including two- and three-component polymers) in batch culture have been determined. The attained polymer yields reached 40-70% of dry cell biomass. The results suggest a conclusion that luminous microorganisms can be considered as producers of multi-component PHAs. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Kalacheva G.S., Rodicheva E.K., Volova T.G.
Заглавие : Synthesis of reserve polyhydroxyalkanoates by luminescent bacteria
Место публикации : Microbiology. - 2008. - Vol. 77, Is. 3. - С. 318-323. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261708030119
Ключевые слова (''Своб.индексиров.''): biosynthesis--chemical structure--luminescent bacteria--polyhydroxyalkanoates (pha)--bacteria (microorganisms)--photobacterium leiognathi--vibrio harveyi
Аннотация: The ability of marine luminescent bacteria to synthesize polyesters of hydroxycarboxylic acids (polyhydroxyalkanoates, PHA) as reserve macromolecules was studied. Twenty strains from the collection of the luminescent bacteria CCIBSO (WDCM839) of the Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, assigned to different taxa (Photobacterium leiognathi, Ph. phosphoreum, Vibrio harveyi, and V. fischeri) were analyzed. The most productive strains were identified, and the conditions ensuring high polymer yields in batch culture (40-70% of the cell dry mass weight) were determined. The capacity for synthesizing two-and three-component polymers containing hydroxybutyric acid as the main monomer and hydroxyvaleric and hydroxyhexanoic acids was revealed in Ph. leiognathi and V. harveyi strains. The results allow luminescent microorganisms to be regarded as new producers of multicomponent polyhydroxyalkanoates. В© 2008 MAIK Nauka.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Kozhevnikov I.V., Steinbuchel A.
Заглавие : Biosynthesis of multicomponent polyhydroxyalkanoates by Wautersia eutropha
Место публикации : Microbiology. - 2007. - Vol. 76, Is. 6. - С. 704-711. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261707060082
Ключевые слова (''Своб.индексиров.''): autotrophic and mixotrophic growth--multicomponent polyhydroxyalkanoates--wautersia eutropha--bacteria (microorganisms)--cupriavidus necator
Аннотация: The effect of carbon supply on polyhydroxyalkanoate (PHA) synthesis by bacteria Wautersia eutropha was studied. Synthesis of multicomponent PHA composed of short-and long-chain monomers (C4-C8) by two natural strains (H16 and B5786) under mixotrophic conditions (CO2 + alkanoic acids as cosubstrates) was demonstrated for the first time. The PHA composition was shown to be dependent on the cosubstrate type. In the presence of odd fatty acids, four-and five-component polymers were synthesized; hydroxybutyrate, hydroxyvalerate, and hydroxyheptanoate were the major monomers, while hydroxyhexanoate and hydroxyoctanoate were minor. In the presence of even fatty acids, PHA contained not only the corresponding molecules (hydroxyhexanoate and hydroxyoctanoate), but also hydroxyvalerate; synthesis of four-component PHA which contain mainly hydroxybutyrate and hydroxyhexanoate (up to 18 mol %) is therefore possible. A series of four-and five-component PHA was synthesized and their physicochemical characteristics were determined. В© 2007 Pleiades Publishing, Ltd.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kalacheva G.S., Volova T.G.
Заглавие : Fatty acid composition of Wautersia eutropha lipids under conditions of active polyhydroxyalkanoates synthesis
Место публикации : Microbiology. - 2007. - Vol. 76, Is. 5. - С. 535-540. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261707050049
Ключевые слова (''Своб.индексиров.''): fatty acids--polyhydroxyalkanoates--wautersia eutropha--cupriavidus necator
Аннотация: The fatty acid composition of the lipids of a Wautersia eutropha polyhydroxyalkanoate-producing strain was studied by chromato-mass spectrometry. A total of 27 fatty acids were identified; their distribution in the cell fractions was determined. In the cytoplasmic membrane, palmitic, palmitoleic, and cis-vaccenic acids were the major components. Long-chain ?-hydroxy acids and myristic acids (components of the lipopolysaccharides of the cell envelope) predominated in the fraction of strongly bound lipids. When the polymer was actively synthesized, the content of cyclopropane acids in the easily extracted lipids increased and the content of the corresponding monoenoic acids decreased. The strongly bound lipids had a high content of long-chain ?-hydroxy acids (more than 50% of the total fatty acids). These results made it possible to determine the source of polyhydroxyalkanoate (PHA) contamination and to choose the strategy for their purification. В© 2007 Pleiades Publishing, Ltd.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Trusova M.Y., Kalacheva G.S., Kozhevnicov I.V.
Заглавие : Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562.
Место публикации : Applied microbiology and biotechnology. - 2006. - Vol. 73, Is. 2. - С. 429-433. - ISSN 01757598 (ISSN) , DOI 10.1007/s00253-006-0460-0
Ключевые слова (''Своб.индексиров.''): beta hydroxyvaleric acid--beta-hydroxyvaleric acid--carbon dioxide--ester--fatty acid--fructose--glucose--hexanoic acid derivative--hydrogen--hydroxyacid--oxygen--polymer--ribosome rna--valeric acid derivative--article--biotechnology--chemistry--metabolism--methodology--wautersia eutropha--biotechnology--carbon dioxide--cupriavidus necator--esters--fatty acids--fructose--glucose--hexanoic acids--hydrogen--hydroxy acids--oxygen--pentanoic acids--polymers--rna, ribosomal
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO(2), fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is beta-hydroxybutyric acid (more than 99 mol %); the identified minor components are beta-hydroxyvaleric acid (0.25-0.72 mol %) and beta-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N.O., Kalacheva G.S., Volova T.G.
Заглавие : Effect of nitrogen limitation on the growth and lipid composition of the green alga Botryococcus braunii Kutz IPPAS H-252
Место публикации : Russian Journal of Plant Physiology. - 2005. - Vol. 52, Is. 3. - С. 311-319. - ISSN 10214437 (ISSN) , DOI 10.1007/s11183-005-0047-0
Ключевые слова (''Своб.индексиров.''): botryococcus--lipids--nitrogen limitation--algae--botryococcus--botryococcus braunii--chlorophyta
Аннотация: The effect of nitrogen limitation in a medium on the composition of intracellular lipids in the alga Botryococcus braunii Kutz IPPAS H-252 in the course of culture development was investigated. Under the conditions of nitrogen limitation, the alga under investigation accumulated lipids as triacylglycerols, and this process was accompanied by substantial changes in the total fatty acid (FA) composition, which were manifested in a decrease in trienoic acids (from 52.8-57.2 to 19.5-24.7% of total FAs) and an increase in the content of oleic (from 1.1-1.2 to 17.1-24.4%) and saturated (from 23.7-26.0 to 32.9-46.1%) acids. In the control culture, the directionality of FA redistribution was less marked, and these changes were noticed at the later stages of culture development. Under nitrogen limitation, marked changes in the FA composition of polar lipids occurred by the 13th day, and they were characterized by an increase in the content of saturated acids (up to 76.8%) and a dramatic decrease in the content of all polyenoic acids (up to 6.8%). The changes in the FA composition of triacylglycerols were noticed as early as by the 7th day; these changes consisted in an increase in the content of oleic acid, and its high content (28.4-38.4%) was maintained up to the end of culturing. In the control culture, triacylglycerols with a high content of oleic acid were found by the 13th day, although, by this time, the content of total lipids and triacylglycerols did not change. В© 2005 MAIK "Nauka/Interperiodica".
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N.O., Kalacheva G.S., Volova T.G.
Заглавие : Influence of nitrogen deficiency on biochemical composition of the green alga Botryococcus
Место публикации : Journal of Applied Phycology. - 2005. - Vol. 17, Is. 4. - С. 309-315. - ISSN 09218971 (ISSN) , DOI 10.1007/s10811-005-7212-x
Ключевые слова (''Своб.индексиров.''): botryococcus--fatty acids--lipids--nitrogen deficiency--biochemical composition--green alga--algae--botryococcus--botryococcus braunii--chlorophyta
Аннотация: A study has been carried out to investigate the influence of nitrogen deficiency on intracellular lipid composition, including total fatty acid composition of lipids, polar lipids, and triacylglycerols, of the alga Botryococcus braunii Kutz IPPAS H-252 in batch culture. Under nitrogen limitation, the alga accumulates lipids as triacylglycerols and the total fatty acid (FA) composition changes: trienoic acids decrease (from 52.8-57.2 to 19.5-24.7% of the total FAs) and the oleic acid increases (from 1.1-1.2 to 17.1-24.4%) as does the saturated acids (from 23.7-26 to 32.9-46.1%). A similar rearrangement in the FA spectrum occurs at later times in the control culture, but it is less pronounced. Under nitrogen limitation, considerable changes in the polar lipid FAs are registered at day 13: saturated acids increase (from 28.6-35.5 to 76.8%) and all polyenoic acids markedly decrease (from 56.9-64.1 to 6.8%). Changes in the triacylglycerol fatty acid spectrum are seen on day 7: the oleic acid increases (from 14.7 to 34.2%) and remains at a high level till the end of the culture. In the control, triacylglycerols with large contents of oleic acid are detected at day 13, the total lipids and triacylglycerols still remaining unchanged. В© Springer 2005.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sevastianov V.I., Perova N.V., Shishatskaya E.I., Kalacheva G.S., Volova T.G.
Заглавие : Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - С. 1029-1042. - ISSN 09205063 (ISSN) , DOI 10.1163/156856203769231547
Ключевые слова (''Своб.индексиров.''): ?-hydroxy acids--endotoxins--hemocompatibility--poly(hydroxybutyrate-co-hydroxyvalerate) (phbv)--polyhydroxyalkanoates (phas)--polyhydroxybutyrate (phb)--bacterium lipopolysaccharide--carbon--complement--copolymer--hydroxyacid--long chain fatty acid--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--valeric acid derivative--adult--article--biofilm--biotechnology--blood analysis--blood clotting--blood compatibility--cell function--chemical analysis--chemical composition--complement activation--concentration (parameters)--controlled study--gas chromatography--hemostasis--human--human cell--mass spectrometry--micromorphology--nonhuman--priority journal--purification--quantitative analysis--sampling--synthesis--thrombocyte adhesion--wautersia eutropha--biocompatible materials--blood--blood coagulation tests--chromatography, gas--complement activation--cupriavidus necator--fatty acids--humans--platelet adhesiveness--polyesters--surface properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Zhila N.O.
Заглавие : Specificity of Lipid Composition in Two Botryococcus Strains, the Producers of Liquid Hydrocarbons
Место публикации : Russian Journal of Plant Physiology. - 2003. - Vol. 50, Is. 5. - С. 627-633. - ISSN 10214437 (ISSN) , DOI 10.1023/A:1025636007023
Ключевые слова (''Своб.индексиров.''): botryococcus--hydrocarbons--mass spectra--structure--algae--botryococcus--botryococcus braunii--chlorophyta--eukaryota
Аннотация: The structure of liquid hydrocarbons and fatty acids produced by the green alga Botryococcus was identified. Two representatives of this alga, Botryococcus braunii Kutz, strain IPPAS H-252, introduced into culture earlier and an organism isolated for the first time from the Shira Lake, were used for this identification. Fatty acid composition of B. braunii, strain H-252, lipids was characterized by a high content of trienoic acids of C16-C18 series. The hydrocarbon composition of this strain was represented by straight-chain and branched-chain C14-C28 components; long-chain linear aliphatic C20-C27 hydrocarbons (54.4%) and 2,6,10,14-tetramethylhexadecane (20.5%) predominated among them. The strain H-252 differed in its fatty acid and hydrocarbon composition from the strains described earlier as Botryococcus braunii. The fatty acid composition of the Botryococcus isolate was represented mainly by C12-C32 saturated and monoenoic acids. The hydrocarbons formed by this isolate were represented by dienoic and trienoic components. C29 (48.9-56.3%) and C31 (11.1-16.3%) hydrocarbons predominated among the C23-C31 dienoic hydrocarbons, and C27, C29, and C31 trienoic hydrocarbons comprised 2.5-2.6% of total hydrocarbons. This type of hydrocarbons and the lipid fatty acid composition were characteristic for the race A of B. braunii.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Kalacheva G.S., Zhila N.O., Gladyshev M.I., Volova T.G.
Заглавие : A Temperature Dependence of the Intra- and Extracellular Fatty-Acid Composition of Green Algae and Cyanobacterium
Место публикации : Russian Journal of Plant Physiology. - 2003. - Vol. 50, Is. 3. - С. 374-380. - ISSN 10214437 (ISSN) , DOI 10.1023/A:1023830405898
Ключевые слова (''Своб.индексиров.''): cyanobacterium--fatty acids--green algae--lipids--secretion--algae--bacteria (microorganisms)--botryococcus braunii--chlorella vulgaris--chlorophyta--eukaryota--prokaryota--spirulina--spirulina platensis
Аннотация: The effect of ambient temperature on the composition of intracellular fatty acids and the release of free fatty acids (FFA) into a medium by cyanobacterium Spirulina platensis and eukaryotic microalgae, Chlorella vulgaris and Botryococcus braunii, was studied using their batch cultures. It was found that all the species studied, regardless of their taxonomic status, responded to the temperature regime by similar changes in their intracellular fatty acid composition: the relative content of more unsaturated fatty acids decreased with the elevation of temperature. At the same time, in the prokaryote, this temperature shift blocked, first of all, the elongation of 16: 0 to 18:0 and then their further desaturation. In eukaryotes, the change in the desaturation of dienoic to trienoic fatty acids was the most pronounced process. The ratio of dienoic to trienoic fatty acids remained almost unchanged in S. platensis. The relative content of extracellular unsaturated FFA increased in the prokaryotic organism S. platensis at a higher temperature. But no significant changes in the composition of extracellular unsaturated FFA were detected in eukaryotic algae upon temperature elevation.
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