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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Semenchenko V.P., Dubovskaya O.P., Fefilova E.B., Makhutova O.N., Buseva Z.F., Sushchik N.N., Razlutskij V.I., Lepskaya E.V., Baturina M.A., Kalachova G.S., Kononova O.N.
Заглавие : Effect of temperature on contents of essential highly unsaturated fatty acids in freshwater zooplankton
Место публикации : Limnologica. - 2011. - Vol. 41, Is. 4. - С. 339-347. - ISSN 00759511 (ISSN) , DOI 10.1016/j.limno.2011.03.001
Ключевые слова (''Своб.индексиров.''): cladocerans--copepods--essential polyunsaturated fatty acids--freshwater zooplankton--water temperature--biomass--climate effect--community structure--fatty acid--freshwater ecosystem--gas chromatography--lake ecosystem--multivariate analysis--seston--taxonomy--temperature effect--trophic level--zooplankton--animalia--copepoda--crustacea
Аннотация: In 11 lakes from cold and warm regions we studied the content of highly unsaturated fatty acids (HUFA) in seston (130?m) and crustacean zooplankton using gas chromatography-mass spectrometry. An increase of temperature correlated with a decrease of HUFA content in zooplankton. A multivariate canonical correlation analyses revealed, that the decrease of HUFA content was related with a decrease of per cent of copepods in zooplankton communities, which are known to have higher HUFA levels in their biomass, than cladocerans. This means that temperature primarily affected the HUFA levels indirectly, via changing of taxonomic structure of zooplankton community, while the homeoviscous adaptation of zooplankton individuals had comparatively lower importance. As found, water temperature was better predictor of HUFA contents of zooplankton, than the fatty acid composition of seston. Thus, it can be predicted, that a probable climate warming will decrease the content of the essential HUFA in freshwater zooplankton with possible negative consequences for animals of higher trophic levels. В© 2011 Elsevier GmbH.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Gubanenko G.A., Demirchieva S.M., Kalachova G.S.
Заглавие : Effect of way of cooking on content of essential polyunsaturated fatty acids in muscle tissue of humpback salmon (Oncorhynchus gorbuscha)
Место публикации : Food Chemistry. - 2006. - Vol. 96, Is. 3. - С. 446-451. - ISSN 03088146 (ISSN) , DOI 10.1016/j.foodchem.2005.02.034
Ключевые слова (''Своб.индексиров.''): antioxidant--docosahexaenoic acid--essential fatty acid--icosapentaenoic acid--omega 3 fatty acid--polyunsaturated fatty acid--animal tissue--conference paper--controlled study--cooking--ecological niche--evolution--fish--fluid intake--food analysis--freezing--frying--heat treatment--heating--hypothesis--market--muscle tissue--nonhuman--oncorrhynchus gorbuscha--reduction--russian federation--salmon--statistical significance--animalia--oncorhynchus gorbuscha--pisces--salmonidae
Аннотация: Contents of fatty acids in filets of unfrozen (control), boiled, fried, roasted and boiled in a small amount of water humpback salmon, collected from a wholesale market in Krasnoyarsk city (Siberia, Russia) were analyzed. Special attention was paid to essential polyunsaturated fatty acids of ?3 family: eicosapentaenoic, 20:5?3 (EPA) and docosahexaenoic, 22:6?3 (DHA). Heat treatment in general did not decrease content of EPA and DHA in humpback, except a modest reduction during frying. Cooked humpback appeared to be the valuable source of essential ?3 PUFAs, namely EPA and DHA. It was hypothesized that the absence of significant reduction of PUFAs' contents in red flesh of fishes of Salmonidae family during heat treatment may be due to a high level of natural antioxidants which formed in the course of evolution as adaptation to their ecological niche. В© 2005 Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zadereev Y.S.
Заглавие : Maternal effects, conspecific chemical cues, and switching from parthenogenesis to gametogenesis in the cladoceran Moina macrocopa
Место публикации : Aquatic Ecology. - 2003. - Vol. 37, Is. 3. - С. 251-255. - ISSN 13862588 (ISSN) , DOI 10.1023/A:1025850417717
Ключевые слова (''Своб.индексиров.''): adaptation--chemical signals--cladocera--gametogenesis induction--maternal control--chemical cue--gametogenesis--maternal effect--parthenogenesis--reproductive strategy--animalia--cladocera--invertebrata--moina--moina macrocopa
Аннотация: The change in reproductive mode from parthenogenesis to gametogenesis in Cladocera is controlled by multiple environmental cues. Maternal effects are involved in the control of reproductive switching. In this study, we estimated the readiness of Moina macrocopa females to change reproduction mode under the effect of conspecific chemicals on maternal and offspring generations. The results demonstrated that information about the chemical environment was not transmitted between generations (none of the females produced diapausing eggs in the control medium irrespective of their mothers' environment). Differences in maternal energetic investments were not significant, hence the maternal environment did not affect the fecundity. However, tested animals adapted to the effect of the diapause inducing factor. When offspring of mothers cultured in crowded water were also cultured in crowded water (the constant effect of conspecific chemicals), they switched less readily to gametogenesis than offsprings of mothers cultured in fresh medium.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T.V., Tikhomirov A.A., Shikhov V.N.
Заглавие : Chlorophyll fluorescence induction and estimation of plant resistance to stress factors
Место публикации : Zhurnal Obshchei Biologii. - 2007. - Vol. 68, Is. 6. - С. 444-458. - ISSN 00444596 (ISSN)
Ключевые слова (''Своб.индексиров.''): chlorophyll--adaptation--cucumber--fluorescence--growth, development and aging--metabolism--physiology--plant leaf--review--adaptation, physiological--chlorophyll--cucumis sativus--fluorescence--plant leaves--cucumis sativus
Аннотация: The usage of chlorophyll fluorescence induction (CFI) for estimating various types of plant resistance (primary, general, initial, adaptive) to stress factors is reviewed. The necessity of ontogenetic approach (considering the age-specific properties of the photosynthetic apparatus) in determining general and adaptive resistance of plants to prolonged action of stress factors by the CFI method is argued. In the plant Cucumbis sativus L., the possibility is shown of using age-specific qualitative and quantitative traits of leaf CFI (changes in the shape of chlorophyll fluorescence induction curves and in the dynamics of CFI parameters in the course of leaf ontogeny) for comparative study of differences between fully active and stressed plants. Possible criteria are suggested for estimating the effect of outer stress factors by the presence or absence of a steady-state phase in the dynamics of CFI parameters during leaf ontogeny. It is also suggested to use the duration of the steady-state phase following the termination of leaf growth (estimated by the dynamics of the slow phase of CFI as the ratio of fluorescence intensity at the peak P and the steady-state fluorescence intensity, FP/FS, or as the viability index Rfd) and the variability of CFI parameters during this period as qualitative estimates of plant resistance to prolonged action of stress factors.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T.V., Tikhomirov A.A.
Заглавие : An ontogenetic approach to the assessment of plant resistance to stress factors based on the method of chlorophyll fluorescence induction.
Место публикации : Doklady. Biochemistry and biophysics. - 2003. - Vol. 388. - С. 4-7. - ISSN 16076729 (ISSN)
Ключевые слова (''Своб.индексиров.''): chlorophyll--adaptation--aging--article--comparative study--cucumber--light--metabolism--methodology--photostimulation--photosynthesis--physiology--plant leaf--radiation dose--radiation exposure--spectrofluorometry--adaptation, physiological--aging--chlorophyll--cucumis sativus--light--photic stimulation--photosynthetic reaction center complex proteins--plant leaves--radiation dosage--spectrometry, fluorescence
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ushakova S.A., Tikhomirov A.A.
Заглавие : Tolerance of LSS plant component to elevated temperatures
Место публикации : Acta Astronautica. - 2002. - Vol. 50, Is. 12. - С. 759-764. - ISSN 00945765 (ISSN) , DOI 10.1016/S0094-5765(02)00010-3
Ключевые слова (''Своб.индексиров.''): metabolism--photosynthesis--rate constants--thermal effects--thermoanalysis--thermal tolerance--life support systems (spacecraft)--carbon dioxide--adaptation--article--brassicaceae--comparative study--heat--instrumentation--light--metabolism--microclimate--photosynthesis--physiology--plant physiology--wheat--adaptation, physiological--brassicaceae--carbon dioxide--ecological systems, closed--heat--life support systems--light--photosynthesis--plant physiology--triticum
Аннотация: Stability of LSS based on biological regeneration of water, air and food subject to damaging factors is largely dependent on the behavior of the photosynthesizing component represented, mainly, by higher plants. The purpose of this study is to evaluate the tolerance of uneven-aged wheat and radish cenoses to temperature effects different in time and value. Estimation of thermal tolerance of plants demonstrated that exposure for 20 h to the temperature increasing to 45В°C brought about irreversible damage both in photosynthetic processes (up to 80% of initial value) and the processes of growth and development. Kinetics of visible photosynthesis during exposure to elevated temperatures can be used to evaluate critical exposure time within the range of which the damage of metabolic processes is reversible. With varying light intensity and air temperature it is possible to find a time period admissible for the plants to stay under adverse conditions without considerable damage of metabolic processes. В© 2002 Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ushakova S.A., Tikhomirov A.A., Volkova E.K., Alekhina E.B., Zavorueva E.N.
Заглавие : Photosynthesis and respiration in plants grown under red and white light
Место публикации : Russian Journal of Plant Physiology. - 1997. - Vol. 44, Is. 3. - С. 317-321. - ISSN 10214437 (ISSN)
Ключевые слова (''Своб.индексиров.''): cucumis sativus--dark respiration--helianthus annuus--line-structured spectrum--lycopersicon esculentum--photosynthesis--plant resistance--red light--respiration in light
Аннотация: Tomato (Lycopersicon esculentum Mill), sunflower (Helianthus annuus L.), and cucumber (Cucumis sativus L.) plants were grown under red or white light at a radiance of 115 W/m2 under controlled conditions. The red spectra were characterized by either two well-distinguished spectral lines (RL1) or only one (RL2). Apparent photosynthesis (Pa), gross photosynthesis (Pg), dark (Rd), and light (R1) respiration, the content of pigments, and the biochemical composition of plants were estimated. Enhancing the destructive red light influence led to a rise in the Pa/R1 and Pa/Pg ratios and to a decrease in chlorophyll a, nitrate, and reduced nitrogen. RL1 was more inhibitory for plants than RL2. It is concluded that declined energy efficiency of respiration contributes to the destructive effect of RL in sunflower and cucumber plants. The rearrangement of metabolic processes, at which energy efficiency of respiration is maintained, is responsible for adaptation of tomato plants to red light. The obtained results allow one to outline the ways to study the species-specific mechanisms of plant responses to prolonged RL action.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tolomeev A.P., Sushchik N.N., Gulati R.D., Makhutova O.N., Kalacheva G.S., Zotina T.A.
Заглавие : Feeding spectra of Arctodiaptomus salinus (Calanoida, Copepoda) using fatty acid trophic markers in seston food in two salt lakes in South Siberia (Khakasia, Russia)
Место публикации : Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - С. 513-530. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-010-9331-y
Ключевые слова (''Своб.индексиров.''): arctodiaptomus salinus--brackish lakes--fatty acids--feeding spectra--salinity adaptation--adaptation--bacterium--brackish water--ciliate--comparative study--crustacean--cyanobacterium--fatty acid--feeding behavior--flagellate--food selection--green alga--lipid--physicochemical property--picoplankton--saline lake--salinity--seasonality--seston--khakassia--russian federation--siberia--animalia--arctodiaptomus salinus--bacillariophyta--bacteria (microorganisms)--calanoida--chlorophyta--ciliophora--copepoda--cryptomonas--cryptophyta--cyanobacteria--mastigophora (flagellates)--proteobacteria
Аннотация: During two vegetation seasons (2004-2005), we compared feeding spectra of Arctodiaptomus salinus (Calanoida, Copepoda) populations inhabiting two neighboring salt lakes, Shira and Shunet, Khakasia, Russia, using fatty acid (FA) trophic markers. Sestonic FA composition in two lakes moderately differed, whereas levels of diatom FA markers were higher in Lake Shunet and of Cyanobacteria and green algae markers in Lake Shira. In general, markers in storage lipids-triacylglycerols (TAG) of A. salinus-reflected the differences in sestonic composition of the two lakes. Nevertheless, TAG fraction was also enriched by FA trophic markers of the minor components of seston, which were selectively ingested by the animals. In Lake Shira, A. salinus had significantly higher concentrations of bacterial FA markers in TAG. In Lake Shunet, TAG of A. salinus contained significantly higher relative amounts of 18:4?3, 18:5?3 and C22 polyunsaturated fatty acids (PUFA), which indicated marked contribution of cryptophytes or (and) flagellates into the diet. Laboratory experiments showed feeding on Cryptomonas and sulfur purple bacteria in Lake Shunet and ciliates and colonial picoplankton in both lakes, and generally confirmed the differences in FA trophic markers in A. salinus between the lakes. The two populations of A. salinus markedly differed in levels of essential long-chain PUFA, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, although the levels of these FA in seston were quite similar between the two lakes. The higher levels of the essential ?3 PUFA in A. salinus in Lake Shunet may be an adaptive response of the animals to a vertical stratification of physico-chemical conditions and significantly higher salinity levels at the boundary of adjacent bottom layer in this lake. В© 2010 Springer Science+Business Media B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Somova L.A., Pechurkin N.S., Pisman T.I.
Заглавие : Principles of biological adaptation of organisms in artificial ecosystems to changes of environmental factors
Место публикации : Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - С. 1512-1515. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2004.12.038
Ключевые слова (''Своб.индексиров.''): artificial ecosystems--biotic cycles--microorganisms--energy utilization--environmental impact--microorganisms--plants (botany)--purification--water--aes--artificial ecosystems--biotic cycles--material transformations--ecosystems--bioremediation--comparative study--conference paper--ecosystem--methodology--microbiology--microclimate--plant--sewage--waste management--water management--biodegradation, environmental--ecological systems, closed--ecosystem--environmental microbiology--life support systems--plants--sewage--soil microbiology--waste management--water purification
Аннотация: Studying material transformations and biotic cycling in artificial ecosystems (AES), we need to know the principles of biological adaptation of active organisms to change in the environment. Microorganisms in AES for water purification are the most active transforming organisms and consumers of the organic substances contained in wastes. Utilization of organic substances is directly connected with the energy fluxes used by AES. According to energy criteria, the energy fluxes used by a biological system tend to reach maximum values under stable conditions. Unutilized substrate concentration decreases as a result of biological adaptations. After a dramatic change in environmental factors, for example, after a sharp increase in the flow rate of organic substances, the biological system is not able to react quickly. The concentration of unutilized substrate increases and the energy flux used by the biological system decreases. The structure of the microbial community also changes, with a decrease in biological diversity. The efficiency of energy use by simple terrestrial ecosystems depends on the energetic intensity and interactions between plants and rhizospheric microorganisms. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Boyandin A.N., Popova L.Yu., Pechurkin N.S.
Заглавие : Experimental evaluation of the processes resulting from the introduction of the transgenic microorganism Escherichia coli Z905/pPHL7 (lux+) into aquatic microcosms
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 7. - С. 1769-1774. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(03)00119-4
Ключевые слова (''Своб.индексиров.''): escherichia coli--genes--sensitivity analysis--aquatic microcosms--space research--genetically modified organism--adaptation, physiological--ampicillin resistance--animals--chemiluminescent measurements--colony count, microbial--daphnia--ecological systems, closed--environmental monitoring--escherichia coli--fresh water--gene expression regulation, bacterial--organisms, genetically modified--plasmids--protozoa--time factors--water microbiology
Аннотация: The processes resulting from the introduction of the transgenic microorganism (TM) E. coli Z905/pPHL7 into aquatic microcosms have been modeled experimentally. It has been shown that the TM E. coli is able to adapt to a long co-existence with indigenous heterotrophic microflora in variously structured microcosms. In more complex microcosms the numerical dynamics of the introduced E. coli Z905/pPHL7 population is more stable. In the TM populations staying in the microcosms for a prolonged time, changes are recorded in the phenotypic expression of plasmid genes (ampicillin resistance and the luminescence level) and chromosome genes (morphological and physiological traits). However, in our study microcosms, the recombinant plasmid persisted in the TM cells for 6 years after the introduction, and as the population adapts to the conditions of the microcosms, the efficiency of the cloned gene expression in the cells is restored. In the microcosms with high microalgal counts (107 cells/ml), cells with a high threshold of sensitivity to ampicillin dominate in the population of the TM E. coli Z905/pPHL7. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Yu., Lobova T.I., Krylova T.Yu., Kargatova T.V., Maksimova E.E., Boyandin A.N., Pechurkin N.S.
Заглавие : Population dynamics of transgenic microorganisms in the different microecosystem conditions
Место публикации : Advances in Space Research. - 2001. - Vol. 27, Is. 9. - С. 1571-1579. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(01)00248-4
Ключевые слова (''Своб.индексиров.''): ecosystems--escherichia coli--genes--interferons--luminescence--vaccines--microcosms--microorganisms--fresh water--microorganism--adaptation--article--bacillus subtilis--comparative study--ecosystem--escherichia coli--gene expression--genetics--growth, development and aging--hazardous waste--microbiology--micrococcus--osmotic pressure--plasmid--population dynamics--risk assessment--transgenic organism--adaptation, physiological--bacillus subtilis--containment of biohazards--ecosystem--escherichia coli--fresh water--gene expression--micrococcus--organisms, genetically modified--osmotic pressure--plasmids--population dynamics--risk assessment--water microbiology
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap1Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human ? 2-interferon Km1Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Lobova T.I., Yu Krylova T., Kargatova T.V., Yu Popova L., Pechurkin N.S.
Заглавие : Effect of salinity on the adaptive capacity of recombinant strains of escherichia coli and bacillus subtilis
Место публикации : Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 243-247. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): genetically modified microorganisms--salinity--salt resistance--sodium chloride--adaptation--article--bacillus subtilis--electrolyte balance--escherichia coli--genetic recombination--genetics--physiology--adaptation, biological--bacillus subtilis--escherichia coli--recombination, genetic--sodium chloride--water-electrolyte balance
Аннотация: Effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wildtype strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : U - Popova LYu, Pechurkin N.S., Maksimova E.E., Kargatova T.V. U - Krylova TYu, Lobova T.I., Boyandin A.N.
Заглавие : Experimental microcosms as models of natural ecosystems for monitoring survival of genetically modified microorganism.
Место публикации : Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - С. 193-197. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial dna--recombinant dna--adaptation--article--ecosystem--escherichia coli--genetics--microbiology--plasmid--risk assessment--adaptation, biological--dna, bacterial--dna, recombinant--ecosystem--escherichia coli--microbiology--plasmids--risk assessment--soil microbiology--water microbiology
Аннотация: An experimental approach for investigation of genetically modified microorganisms (GMMO) introduced into model ecosystems to evaluate potential risk of propagation of recombinant plasmids in surrounding medium has been developed. The object of modeling was Escherichia coli Z905 strain with a recombinant plasmid with bacterial luminescence genes, which was introduced into water microcosms of different structure. The approach involves comprehensive investigation of GMMO at four hierarchical levels: molecular (retaining the structure of the plasmid and expression of cloned genes); cellular (variation of metabolic activity); population (competitive power and metabolic interactions of GMMO with indigenous microflora, migration of recombinant and natural plasmids); ecosystem (effect of GMMO and cloned genes on ecosystem parameters). The experimental evidence and theoretical estimates are intended to form grounds to develop a basic version of an ecological certificate for different GMMO variants.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Popova L.Y.
Заглавие : Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2001. - Vol. 46, Is. 2. - С. 251-255. - 5. - ISSN 0006-3029
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): bacterial luminescence--recombinant plasmid--salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson I.I., Terskov I.A., Kovrov B.G., Sidko F.Ya., Lisovsky G.M., Okladnikov Yu.N., Belyanin V.N., Trubachov I.N., Rerberg M.S.
Заглавие : Life support system with autonomous control employing plant photosynthesis
Место публикации : Acta Astronautica. - 1976. - Vol. 3, Is. 9-10. - С. 633-650. - ISSN 00945765 (ISSN)
Ключевые слова (''Своб.индексиров.''): biochemical engineering - photosynthesis--spacecraft--adaptation--article--chlorella--growth, development and aging--human--instrumentation--intestine--male--metabolism--microbiology--microclimate--photosynthesis--physiology--plant--space flight--task performance--vegetable--water supply--wheat--adaptation, physiological--chlorella--ecological systems, closed--environment, controlled--humans--intestines--life support systems--male--metabolism--photosynthesis--plants--space simulation--task performance and analysis--triticum--vegetables--water supply
Аннотация: This research was aimed at obtaining a closed control system. This was achieved by placing all the technological processes providing for human vital activities within the hermetically sealed space, and by transferring the entire control and guidance of these processes to people inhabiting the system. In contrast to existing biological life support systems, man has been included not only as a participant of metabolism, but as an operator who is the central figure in collecting information, making decisions and controlling all technological processes. To tackle this problem, the "BIOS-3" experimental complex was created for performing long-term experiments using different structures of biological life-support system. The experiment lasted six months and consisted of three stages. During the first stage the system was comprised of two equivalent phytotrons with the culture of wheat and an assortment of vegetable plants, and the living compartment. At the second stage, one of the phytotrons was removed while a compartment of chlorella cultivators was introduced. The third stage differed from the second, the former using wheat phytotron and the latter employing phytotron with an assortment of vegetable cultures. Three men inhabited the system simultaneously. The experiment demonstrated that a biological life support system controlled autonomously from the inside is feasible within a small confined space. However, immunological and microbiological research shows, that the medium created by the system is not fully adequate for man. In conclusion, some prospects have been outlined for further studies of biological life support systems. В© 1976.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Y., Kargatova T.V., Maksimova E.E., Belyavskaya V.A.
Заглавие : A study on the possibility of environmental adaptation of a Bacillus subtilis strain containing a recombinant plasmid with the gene of human interferon alpha 2
Колич.характеристики :5 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 6. - P637-641. - ISSN 0026-2617
Примечания : Cited References: 13
Предметные рубрики: GENETICALLY-MODIFIED MICROORGANISMS
Ключевые слова (''Своб.индексиров.''): microecosystem--bacillus subtilis--recombinant plasmid--interferon--adaptation
Аннотация: Adaptation of the Bacillus subtilis strain 2335/105 (Km(r)Inf(+)) containing a recombinant plasmid encoding the extracellular human interferon alpha 2 was studied under various conditions. Stability of the plasmid in the population of B. subtilis 2335/105 was estimated under nonselective conditions. The plasmid-free cells and cells with a low number of plasmid copies were found to accumulate progressively, constituting 80% of the population after 10 culture passages, indicating the poor competitiveness of cells carrying a high number of plasmid copies. The behavior of vegetative cells of the recombinant strain introduced into aquatic microcosms differing in trophic chain length was studied. Within the first 10 days, the lysis of vegetative cells of B. subtilis 2335/105 occurred; the number of viable spores was very low but remained constant for half a year.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Popova L.Y.
Заглавие : Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051
Колич.характеристики :5 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2001. - Vol. 46, Is. 2. - P251-255. - ISSN 0006-3029
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): bacterial luminescence--recombinant plasmid--salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.
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18.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Popova L.Y., Lobova T.I., Krylova T.Y., Kargatova T.V., Maksimova E.E., Boyandin A.N., Pechurkin N.S.
Заглавие : Population dynamics of transgenic microorganisms in the different microecosystem conditions
Колич.характеристики :9 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH: ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P1571-1579. - ISBN 0273-1177, DOI 10.1016/S0273-1177(01)00248-4
Примечания : Cited References: 8
Предметные рубрики: GENETICALLY-ENGINEERED MICROORGANISMS
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap(r)Lux(+)) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human alpha 2-interferon Km(r)lnf(+)), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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19.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Lankin J.P.
Заглавие : Adaptive simulation of atmospheric phenomena
Колич.характеристики :12 с
Место публикации : EIGHTH INTERNATIONAL SYMPOSIUM ON ATMOSPHERIC AND OCEAN OPTICS: ATMOSPHERIC PHYSICS. Ser. PROCEEDINGS OF THE SOCIETY OF PHOTO-OPTICAL INSTRUMENTATION ENGINEERS (SPIE): SPIE-INT SOC OPTICAL ENGINEERING, 2002. - Vol. 4678: 8th International Symposium on Atmospheric and Ocean Optics - Atmospheric Physics (JUN 25-29, 2001, IRKUTSK, RUSSIA). - P668-679. - ISBN 0277-786X, DOI 10.1117/12.458507. - ISBN 0-8194-4433-2
Примечания : Cited References: 46
Ключевые слова (''Своб.индексиров.''): atmosphere--system--adaptation--self-adaptation--neuroinformatics
Аннотация: The paper describes scientific methodology of developing complex nonlinear dynamic models which form the basis of a new scientific trend called Stikhioniks(1) and are a efficient tool to devise atmosphere models. The model is aimed to develop complexly organized hierarchical non-equilibrium adaptive systems with a wide range of connections and elements and is a new step as related to the now dominant mechanical-statistical paradigm.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zadereev Y.S.
Заглавие : Maternal effects, conspecific chemical cues, and switching from parthenogenesis to gametogenesis in the cladoceran Moina macrocopa
Колич.характеристики :6 с
Место публикации : Aquat. Ecol.: KLUWER ACADEMIC PUBL, 2003. - Vol. 37, Is. 3. - P251-255. - ISSN 1386-2588, DOI 10.1023/A:1025850417717
Примечания : Cited References: 19
Предметные рубрики: POPULATION-DENSITY
DAPHNIA-MAGNA
CRUSTACEA
REPRODUCTION
DIAPAUSE
Ключевые слова (''Своб.индексиров.''): adaptation--cladocera--chemical signals--gametogenesis induction--maternal control
Аннотация: The change in reproductive mode from parthenogenesis to gametogenesis in Cladocera is controlled by multiple environmental cues. Maternal effects are involved in the control of reproductive switching. In this study, we estimated the readiness of Moina macrocopa females to change reproduction mode under the effect of conspecific chemicals on maternal and offspring generations. The results demonstrated that information about the chemical environment was not transmitted between generations (none of the females produced diapausing eggs in the control medium irrespective of their mothers' environment). Differences in maternal energetic investments were not significant, hence the maternal environment did not affect the fecundity. However, tested animals adapted to the effect of the diapause inducing factor. When offspring of mothers cultured in crowded water were also cultured in crowded water (the constant effect of conspecific chemicals), they switched less readily to gametogenesis than offsprings of mothers cultured in fresh medium.
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