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Population dynamics of transgenic microorganisms in the different microecosystem conditions / L. Yu. Popova [et al.] // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1571-1579, DOI 10.1016/S0273-1177(01)00248-4 . - ISSN 0273-1177
Кл.слова (ненормированные):
Ecosystems -- Escherichia coli -- Genes -- Interferons -- Luminescence -- Vaccines -- Microcosms -- Microorganisms -- fresh water -- microorganism -- adaptation -- article -- Bacillus subtilis -- comparative study -- ecosystem -- Escherichia coli -- gene expression -- genetics -- growth, development and aging -- hazardous waste -- microbiology -- Micrococcus -- osmotic pressure -- plasmid -- population dynamics -- risk assessment -- transgenic organism -- Adaptation, Physiological -- Bacillus subtilis -- Containment of Biohazards -- Ecosystem -- Escherichia coli -- Fresh Water -- Gene Expression -- Micrococcus -- Organisms, Genetically Modified -- Osmotic Pressure -- Plasmids -- Population Dynamics -- Risk Assessment -- Water Microbiology
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap1Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human ? 2-interferon Km1Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Lobova, T.I.; Krylova, T.Yu.; Kargatova, T.V.; Maksimova, E.E.; Boyandin, A.N.; Pechurkin, N.S.

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Mathematical modeling of population dynamics of unstable plasmid-containing bacteria during continuous cultivation in a chemostat / V. V. Ganusov, A. V. Bril'kov, N. S. Pechurkin // Biofizika. - 2000. - Vol. 45, Is. 5. - С. 908-914 . - ISSN 0006-3029
Кл.слова (ненормированные):
article -- bioreactor -- Escherichia coli -- fermentation -- genetics -- growth, development and aging -- Photobacterium -- plasmid -- theoretical model -- Bioreactors -- Escherichia coli -- Fermentation -- Models, Theoretical -- Photobacterium -- Plasmids
Аннотация: A structural approach to studying the regularities of the population dynamics of unstable recombinant bacterial strains in a chemostat was elaborated. The approach is based on the mathematical modeling of cell distribution in a population with different numbers of plasmid copies. The effect of decreased selective preference of plasmidless variants of the recombinant strain in the chemostat, which is related to a decrease in the number of plasmid copies in cells upon long-term incubation was analyzed. It is shown that the time of half-elimination of plasmids from the bacterial population in the steady state in the chemostat T1/2 does not depend on the maximum number of plasmid copies in cells N but is determined only by the mean time of generation g and the probability of the loss of one plasmid copy tau. The dependence of the preference of bacterial plasmidless variants on the efficiency of expression of genes cloned into plasmids in chemostat was analyzed using the recombinant strain E. coli Z905, whose plasmids pPHL-7 contain cloned genes for the luminescence system of marine luminescing bacteria Photobacterium leiognathi.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Ganusov, V.V.; Bril'kov, A.V.; Pechurkin, N.S.

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On monitoring the bacterial component as an indicator of the state of small man-made ecosystems / A. B. Sarangova, L. A. Somova, N. S. Pechurkin // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1605-1609, DOI 10.1016/S0273-1177(01)00256-3 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bacteria -- Ecosystems -- Substrates -- Intracellular substrate concentration -- Space research -- catalase -- oxidoreductase -- artificial ecosystem -- article -- bacterial phenomena and functions -- biomass -- culture medium -- ecosystem -- enzymology -- growth, development and aging -- metabolism -- microbiology -- oxygen consumption -- Pseudomonas -- Bacterial Physiology -- Biomass -- Catalase -- Culture Media -- Ecosystem -- Oxidoreductases -- Oxygen Consumption -- Pseudomonas -- Water Microbiology
Аннотация: High reproduction rates make the bacterial component of ecosystems a good indicator of the state of the system on the whole. This determines the necessity to develop rapid monitoring of the functional state of the bacterial component of small ecosystems. Information about substrate concentration in the population is indicative of the state of the bacterial culture. Conventional methods of monitoring the concentration of integral substrate in the system take time much longer than the changes in the ecosystem. The paper presents theoretical foundations for the logical sequence "catalase activity - intracellular substrate concentration - estimate of substrate consumed by bacteria" for experimental verification and as a consequence of development of the integral method of monitoring the bacterial population on the basis of determining bacterial catalase activity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Academgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sarangova, A.B.; Somova, L.A.; Pechurkin, N.S.

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Effect of bacterial population density on germination wheat seeds and dynamics of simple artificial ecosystems / L.A Somova [et al.] // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1611-1615, DOI 10.1016/S0273-1177(01)00257-5 . - ISSN 0273-1177
Кл.слова (ненормированные):
Bacteria -- Biomass -- Ecosystems -- Plants (botany) -- Seed -- Artificial soil -- Germination -- Photoassimilation -- Space research -- artificial ecosystem -- article -- comparative study -- ecosystem -- germination -- growth, development and aging -- isolation and purification -- microbiology -- physiology -- plant root -- plant seed -- Pseudomonas fluorescens -- Pseudomonas putida -- wheat -- Ecosystem -- Germination -- Plant Roots -- Pseudomonas fluorescens -- Pseudomonas putida -- Seeds -- Triticum
Аннотация: Effect of the size of rhizospheric bacterial populations on germination of seeds and development of simple terrestrial "wheat plants - rhizospheric microorganisms - artificial soil" and "wheat plants - artificial soil" systems has been studied. Experiments demonstrated that within specify ranges in the inoculate, the rhizospheric bacteria are capable of increasing the yield of germinated seeds and stimulate the growth of plantlets. Germination of seeds inoculated with bacteria was either stimulated, or inhibited or remained at control levels depending on the amount of bacteria. Plant biomass growth and total photoassimilation has been found to depend on the amount of bacteria on the plant roots: the higher the amount of bacteria on plant roots, the smaller is the biomass of plants but the total photoassimilation is, higher. Thus, depending on the amount of bacteria on the roots of plants the system either increases the biomass of plants or increases the total photoassimilation, i.e. "pumps" carbon through itself involving bacteria. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Inst. of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A; Pechurkin, N.S.; Sarangova, A.B.; Pisman, T.I.

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A mathematical model of "plants-microorganisms" interaction on complete mineral medium and under nitrogen limitation / T. I. Pisman [et al.] // Advances in Space Research. - 1999. - Vol. 24, Is. 3. - P383-387 . - ISSN 0273-1177
Кл.слова (ненормированные):
nitrogen -- ecological modeling -- interspecific interaction -- nutrient limitation -- plant -- rhizosphere -- article -- biological model -- biomass -- comparative study -- culture medium -- drug effect -- growth, development and aging -- mathematics -- microbiology -- plant root -- Pseudomonas fluorescens -- sweating -- wheat -- Biomass -- Culture Media -- Mathematics -- Models, Biological -- Nitrogen -- Plant Roots -- Plant Transpiration -- Pseudomonas fluorescens -- Triticum
Аннотация: A mathematical model concerning the interaction of plants and rhizospheric microorganisms on complete mineral medium and under nitrogen limitation has been constructed. The model takes into account the closeness of plants and microorganisms in terms of the matter released by the plant and consumed by the microorganisms. The effect of rhizospheric microorganisms on plant growth with normal carbon dioxide and complete mineral medium has been demonstrated. Plants interacting with microorganisms have a greater biomass than plants growing without microorganisms. Wheat growth stimulation by metabolites of rhizospheric microorganisms under laboratory conditions on artificial soil has been experimentally demonstrated (Pechurkin, 1997). Under nitrogen limitation , the biomass of plants, with or without microorganisms, is identical, and is substantially reduced as compared with the medium with standard nitrogen. В© 1999 COSPAR. Published by Elsevier Science Ltd.

Scopus
Держатели документа:
Institute of Biophysics (Siberian Branch, Russian Academy of Sciences), Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pisman, T.I.; Pechurkin, N.S.; Mariasova, T.S.; Somova, L.A.; Sarangova, A.B.

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Small artificial ecosystems: response to variation of environmental factors (CO2 enrichment). / L. A. Somova [et al.] // Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - P215-220 . - ISSN 1069-9422
Кл.слова (ненормированные):
carbon -- carbon dioxide -- article -- biomass -- comparative study -- drug effect -- ecosystem -- growth, development and aging -- metabolism -- microbiology -- microclimate -- photosynthesis -- plant seed -- Pseudomonas -- wheat -- Biomass -- Carbon -- Carbon Dioxide -- Ecosystem -- Environment, Controlled -- Photosynthesis -- Pseudomonas -- Seeds -- Soil Microbiology -- Triticum
Аннотация: Response of "wheat plants--rhizospheric microorganisms--artificial soil"--a simple terrestrial ecosystem--to carbon dioxide increased in its atmosphere to 0.06% has been studied. It has been experimentally demonstrated that a simple ecosystem develops and functions different from its individual elements (components), in this case "plants-artificial soil" without microorganisms. With mineral nutrition unlimited and CO2 enrichment the system is capable of binding (involving into turnover) 40% more carbon than the system without microorganisms. With material balance as the basis, this article evaluates the contribution of a system's elements into its development, namely, the contribution of the photosynthesizing component and the contribution of microorganisms.

Scopus
Держатели документа:
Institute of Biophysics, (Russian Academy of Sciences, Siberian Branch), Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.; Sarangova, A.B.; Pisman, T.I.; Polonsky, V.I.; Sadovskay, G.M.

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Plants-rhizospheric organisms interaction in a manmade system with and without biogenous element limitation / L. A. Somova [et al.] // Advances in Space Research. - 1997. - Vol. 20, Is. 10. - P1939-1943 . - ISSN 0273-1177
Кл.слова (ненормированные):
nitrogen -- article -- bacterial count -- biomass -- comparative study -- culture medium -- growth, development and aging -- metabolism -- microbiology -- plant leaf -- plant root -- plant seed -- Pseudomonas fluorescens -- Pseudomonas putida -- wheat -- Biomass -- Colony Count, Microbial -- Culture Media -- Nitrogen -- Plant Leaves -- Plant Roots -- Pseudomonas fluorescens -- Pseudomonas putida -- Seeds -- Triticum
Аннотация: The effect has been studied of inoculation of seeds of wheat with two species of rhizospheric microorganisms, -Pseudomonas fluorescens and Pseudomonas putida - on young plant growth with complete and with nitrogen deficit mineral nutrition. With complete mineral medium, plants grown from seeds inoculated with bacteria of Pseudomonas genus (experiment plants) have been found to have better growth over plants not inoculated with these bacteria (control plants). The experiment plants had increased transpiration and their biomass had higher organic nitrogen content. With nitrogen deficit medium, the plants inoculated with bacteria and those without them, have not revealed changes in growth. Neither case demonstrated competition of microorganisms with plants for nitrogen sources. В© 1997 COSPAR. Published by Elsevier Science Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk 6600036, Russian Federation
CEA-Sciences du Vivant, DEVM, CEA/Cadarache, Saint-Paul-Lez-Durance Cedex, France
Computing Center Russian, Krasnoyarsk 6600036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.; Polonsky, V.I.; Pisman, T.I.; Sarangova, A.B.; Andre, M.; Sadovskaya, G.M.

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Modelling of genetically engineered microorganisms introduction in closed artificial microcosms / N. S. Pechurkin [et al.] // Advances in Space Research. - 1999. - Vol. 24, Is. 3. - P335-341, DOI 10.1016/S0273-1177(99)00320-8 . - ISSN 0273-1177
Кл.слова (ненормированные):
aquatic environment -- artificial ecosystem -- ecological modeling -- genetically modified organism -- alga -- animal -- article -- bacterial count -- bacterial gene -- biological model -- biomass -- Escherichia coli -- feasibility study -- genetic engineering -- genetics -- growth, development and aging -- microbiology -- microclimate -- Photobacterium -- plasmid -- protozoon -- time -- yeast -- Algae -- Animals -- Biomass -- Colony Count, Microbial -- Ecological Systems, Closed -- Escherichia coli -- Feasibility Studies -- Genes, Bacterial -- Genetic Engineering -- Models, Biological -- Photobacterium -- Plasmids -- Protozoa -- Time Factors -- Water Microbiology -- Yeasts
Аннотация: The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Ap(r), Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E.coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E.coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Pechurkin, N.S.; Brilkov, A.V.; Ganusov, V.V.; Kargatova, T.V.; Maksimova, E.E.; Popova, L.Yu.

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Bios-3: Siberian experiments in bioregenerative life support / F. B. Salisbury, J. I. Gitelson, G. M. Lisovsky // BioScience. - 1997. - Vol. 47, Is. 9. - P575-585 . - ISSN 0006-3568
Кл.слова (ненормированные):
agriculture -- Chlorella -- construction work and architectural phenomena -- crop -- energy metabolism -- evaluation -- growth, development and aging -- human -- metabolism -- methodology -- microbiology -- microclimate -- NASA Discipline Life Support Systems -- Non-NASA Center -- photon -- review -- Russian Federation -- space flight -- NASA Discipline Life Support Systems -- Non-NASA Center -- Agriculture -- Chlorella -- Crops, Agricultural -- Ecological Systems, Closed -- Energy Metabolism -- Environment, Controlled -- Environmental Microbiology -- Evaluation Studies -- Facility Design and Construction -- Humans -- Life Support Systems -- Photons -- Siberia -- Space Flight -- Space Simulation

Scopus
Держатели документа:
Dept. Plants, Soils, Biometeorology, College of Agriculture, Utah State University, Logan, UT 84322-4820, United States
Institute of Biophysics, Academy of Sciences of Russia, Siberian Branch, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Salisbury, F.B.; Gitelson, J.I.; Lisovsky, G.M.

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10.

Man as a component of a closed ecological life support system / J. I. Gitelson, U - Okladnikov YuN // Life support & biosphere science : international journal of earth space. - 1994. - Vol. 1, Is. 2. - P73-81 . - ISSN 1069-9422
Кл.слова (ненормированные):
carbon dioxide -- oxygen -- article -- blood analysis -- blood cell count -- breathing -- construction work and architectural phenomena -- crop -- diet -- growth, development and aging -- human -- metabolism -- microclimate -- physiology -- Russian Federation -- space flight -- standard -- waste management -- water supply -- Blood Cell Count -- Blood Chemical Analysis -- Carbon Dioxide -- Crops, Agricultural -- Diet -- Ecological Systems, Closed -- Environment, Controlled -- Facility Design and Construction -- Humans -- Life Support Systems -- Oxygen -- Respiration -- Russia -- Space Flight -- Waste Management -- Water Supply
Аннотация: Material support of all manned space flights so far has been provided from a prestored stock of substances or replenished from the Earth's biosphere. Exploration of space will, however, become real only when man is able to break away from Earth completely, when he will be accompanied by a system providing everything necessary to sustain full-valued life for an unlimited time. The only known system to date meeting this requirement is the Earth's biosphere. To break away from his cradle, as K.E. Tsiolkovsky called Earth, it is necessary to devise a life support system functionally similar to the natural biosphere. This need not be similar in structure to the vast diversity of trophic relationships available on Earth, but requires the solution of a multitude of various problems of an ecological, physiological, engineering and social-psychological nature. Human life-support systems based on biological regeneration of environments in small volumes have been studied at the Institute of Biophysics (Siberian Branch of the Russian Academy of Sciences) over many years. This work has resulted in the design of Bios-3, a biologically-based self-sustained human life support system.

Scopus
Держатели документа:
Institute of Biophysics (Siberian Branch of Russian Academy of Sciences), Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, J.I.; , U - Okladnikov YuN

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11.

Biological-physical-chemical aspects of a human life support system for a lunar base / J. I. Gitelson [et al.] // Acta Astronautica. - 1995. - Vol. 37, Is. C. - P385-394 . - ISSN 0094-5765
Кл.слова (ненормированные):
animal -- aquaculture -- article -- biomass -- construction work and architectural phenomena -- Cyprinodontiformes -- filtration -- growth, development and aging -- human -- microbiology -- microclimate -- moon -- nutritional value -- photoperiodicity -- plant -- space flight -- standard -- Tilapia -- waste management -- water management -- wheat -- Animals -- Aquaculture -- Biomass -- Cyprinodontiformes -- Ecological Systems, Closed -- Facility Design and Construction -- Filtration -- Humans -- Life Support Systems -- Moon -- Nutritive Value -- Photoperiod -- Plants, Edible -- Space Flight -- Tilapia -- Triticum -- Waste Management -- Water Microbiology -- Water Purification
Аннотация: To create a life support system based on biological and physical-chemical processes is the optimum solution providing full-valued condidtions for existence and efficient work of people at a lunar base. Long-standing experinece in experimental research or closed ecosystems and their components allows us to suggest a realistic functional structure of the lunar base and to estimate qualitatively its parameters. The original restrictions are as follows: 1) the basic source of energy to support the biological processes has to be the solar radiation; 2) the initial amount of basic biological elelments forming the turnover of substances (C, O, H, P, K, N) has to be delivered from Earth; 3). Moon materials are not to be used in the biological turnover inside the base; 4) the base is to supply the crew fully with atmosphere and water, and with 90% (A scenario) or 40% (B scenario) of food. Experimental data about the plant productivity under the "Moon" rhythm of light and darkness allow us to suggest that the A scenario requires per one human: plant area - 40 m2 irradiated during the lunar day by 250-300 W/m2 PAR producing 1250 g of dry biomass a terrestrial day; a heterotrophic component of "biological incineration" of inedible plant biomass (800 g/day) including the aquaculture of fish to produce animal products and contaminating the environment less than birds and mammals, and the culture of edible mushrooms; a component of physical-chemical correction for the LSS envi ronment including the subsystems of: deep oxidation of organic impurities in the atmosphere and of water, organic wastes of human activity and that biological components (420 g/day) Co2 concentration in "Moon" nights, damping O2 in "Moon" days, etc. The stock of presotred or delivered from Earth substances (food additions, seeds, etc.) to be involved in biological turnover is to be about 50 kg/year per man. Increase of the mass of prestored substances per man up to 220 kg/year would reduce twice the plant area and consumed amount of radiant energy to exclude the components of "biological incineration" and physical-chemical destruction of organic wastes. В© 1995.

Scopus
Держатели документа:
Institute of Biophysics (Russian Academy of Sciences, Siberian Branch) Krasnoyarsk, Russian Federation
Ruhr-University of Bochum, C.E.B.A.S. Center of Excellence., Bochum, Germany
Institute of Medical-Biological Problems, Moscow, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, J.I.; V, B.; Grigoriev, A.I.; Lisovsky, G.M.; Manukovsky, N.S.; Sinyak, Y.u.E.; Ushakova, S.A.

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12.

Impaired growth of plants cultivated in a closed system: Possible reasons / J. I. Gitelson [et al.] // Advances in Space Research. - 1997. - Vol. 20, Is. 10. - P1927-1930 . - ISSN 0273-1177
Кл.слова (ненормированные):
air conditioning -- air pollutant -- article -- culture medium -- growth, development and aging -- human -- hydroponics -- methodology -- microbiology -- microclimate -- plant -- sewage -- wheat -- Air Conditioning -- Air Pollutants -- Culture Media -- Ecological Systems, Closed -- Environment, Controlled -- Humans -- Hydroponics -- Life Support Systems -- Plants -- Triticum -- Waste Disposal, Fluid -- Water Microbiology
Аннотация: Plants in experiments on "man-higher plants" closed ecosystem (CES) have been demonstrated to have inhibited growth and reduced productivity due to three basic factors: prolonged usage of a permanent nutrient solution introduction into the nutrient medium of intra-system gray water, and closure of the system. Gray water was detrimental to plants the longer the nutrient solution was used. However, higher plant growth was mostly affected by the gaseous composition of the CES atmosphere, through accumulation of volatile substances. В© 1997 COSPAR. Published by Elsevier Science Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, J.I.; Tirranen, L.S.; Borodina, E.V.; Rygalov, V.Ye.

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13.

Survival and alteration of the plasmid-containing microorganism Escherichia coli Z905/pPHL7 introduced into manmade closed aquatic microcosms / A. N. Boyandin [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1763-1768, DOI 10.1016/S0273-1177(03)00118-2 . - ISSN 0273-1177
Кл.слова (ненормированные):
Cells -- Escherichia coli -- Salts -- Recombinant plamid -- Space research -- ampicillin -- chloride -- inorganic salt -- magnesium -- mineral -- potassium -- sodium -- sulfate -- genetically modified organism -- article -- bacterial count -- chemoluminescence -- culture medium -- drug effect -- Escherichia coli -- genetics -- growth, development and aging -- microbiology -- microclimate -- penicillin resistance -- plasmid -- Russian Federation -- transgenic organism -- Ampicillin -- Ampicillin Resistance -- Chemiluminescent Measurements -- Chlorides -- Colony Count, Microbial -- Culture Media -- Ecological Systems, Closed -- Escherichia coli -- Magnesium -- Minerals -- Organisms, Genetically Modified -- Plasmids -- Potassium -- Russia -- Salts -- Sodium -- Sulfates -- Water Microbiology
Аннотация: It has been demonstrated that the transgenic microorganism Escherichia coli Z905/pPHL7 (Ap'Lux+) can exist for a long time at an elevated concentration of mineral salts. The microorganism was introduced into microcosms with sterile brackish water (salinity variable from 21 to 22 g 1-1) taken from Lake Shira (Khakasia, Russia). The survivof the microorganism was estimated both by measuring the growth of the colonies on solid nutrient media and by the bioluminescence exhibited by the transgenic strain in samples from the microcosms and in the enrichment culture with the added selective factor - ampicillin (50 ?g/ml). In the enrichment culture, the bioluminescent signal was registered through the 160-day experiment. It has been shown that in the closed microcosms with brackish water the E. coli strain becomes heterogeneous in its ampicillin resistance. The populations of the transgenic strain were mainly represented by isolates able to persist in the medium containing 50 ?g/ml, but there were also the cells (about 10%) with the threshold of ampicillin resistance not more than 0.05 ?g/ml. Thus, it was shown that in the microcosms with brackish water and in the absence of the selective factor the transgenic strain survives and retains the recombinant plasmid. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Popova, L.Yu.; Pechurkin, N.S.

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14.

Increase of atmospheric CO2: Response patterns on a simple terrestrial man-made ecosystem / L. A. Somova, N. S. Pechurkin, T. I. Pisman // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1731-1735, DOI 10.1016/S0273-1177(03)80019-4 . - ISSN 0273-1177
Кл.слова (ненормированные):
Carbon dioxide -- Earth atmosphere -- Ecosystems -- Environmental impact -- Mathematical models -- Microorganisms -- Plants (botany) -- Soils -- Man-made ecosystems -- Space research -- biosphere -- carbon dioxide enrichment -- terrestrial ecosystem -- carbon dioxide -- nitrogen -- phosphorus -- article -- atmosphere -- biological model -- chemistry -- dose response -- drug effect -- ecosystem -- growth, development and aging -- metabolism -- microbiology -- microclimate -- plant root -- wheat -- Atmosphere -- Carbon Dioxide -- Dose-Response Relationship, Drug -- Ecosystem -- Environment, Controlled -- Models, Biological -- Nitrogen -- Phosphorus -- Plant Roots -- Soil Microbiology -- Triticum
Аннотация: Simple models of terrestrial ecosystems with a limited number of components are an efficient tool to study the main laws of functioning of populations, including microbial ones, and their communities, as components of natural ecosystems, under variable environmental conditions. Among other factors are the increase of carbon dioxide in the atmosphere and limitation of plants' growth by biogenic elements. The main types of ecosystems' responses to changes in environmental conditions (a change in CO2 concentration) have been demonstrated in a "plants - rhizospheric microorganisms - artificial soil" simple experimental system. The mathematical model of interactions between plants and microorganisms under normal and elevated atmospheric CO2 and limitation by nutrients (nitrogen and phosphorus) yielded a qualitative agreement between calculated and experimental values of limiting substances concentrations and release rates of exudates. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.; Pisman, T.I.

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15.

Population dynamics of transgenic strain Escherichia coli Z905/pPHL7 in freshwater and saline lake water microcosms with differing microbial community structures / L. Yu. Popova [et al.] // Advances in Space Research. - 2005. - Vol. 35, Is. 9 SPEC. ISS. - P1573-1578, DOI 10.1016/j.asr.2005.01.037 . - ISSN 0273-1177
Кл.слова (ненормированные):
Microcosm -- Plasmid -- Population dynamics -- Transgenic microorganism -- Bacteria -- Genes -- Microorganisms -- Saline water -- Microcosms -- Plasmid -- Population dynamics -- Transgenic microorganism -- Escherichia coli -- brine -- fresh water -- inorganic salt -- bacterial gene -- conference paper -- ecosystem -- Escherichia coli -- gene expression regulation -- genetics -- growth, development and aging -- microbiology -- Micrococcus -- penicillin resistance -- plasmid -- population dynamics -- transgenic organism -- Ampicillin Resistance -- Ecosystem -- Environmental Microbiology -- Escherichia coli -- Fresh Water -- Gene Expression Regulation, Bacterial -- Genes, Bacterial -- Micrococcus -- Organisms, Genetically Modified -- Plasmids -- Population Dynamics -- Salts -- Water Microbiology
Аннотация: Populations of Escherichia coli Z905/pPHL7, a transgenic microorganism, were heterogenic in the expression of plasmid genes when adapting to the conditions of water microcosms of various mineralization levels and structure of microbial community. This TM has formed two subpopulations (ampicillin- resistant and ampicillin-sensitive) in every microcosm. Irrespective of mineralization level of a microcosm, when E. coli Z905/pPHL7 alone was introduced, the ampicillin-resistant subpopulation prevailed, while introduction of the TM together with indigenous bacteria led to the dominance of the ampicillin-sensitive subpopulation. A high level of lux gene expression maintained longer in the freshwater microcosms than in sterile saline lake water microcosms. A horizontal gene transfer has been revealed between the jointly introduced TM and Micrococcus sp. 9/pSH1 in microcosms with the Lake Shira sterile water. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Laboratory of Controlled Heterotroph Biosynthesis, Institute of Biophysics, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation
Siberian Branch, Institute of Computational Modeling, Russian Academy of Sciences, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Kargatova, T.V.; Ganusova, E.E.; Lobova, T.I.; Boyandin, A.N.; Mogilnaya, O.A.; Pechurkin, N.S.

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16.

Trends in microevolution of microbial populations in open systems / A. V. Brilkov [et al.] // Doklady Biochemistry and Biophysics. - 2005. - Vol. 404, Is. 1-6. - P349-352, DOI 10.1007/s10628-005-0111-x . - ISSN 1607-6729
Кл.слова (ненормированные):
article -- bacterial phenomena and functions -- bacterium -- biological model -- culture technique -- Escherichia coli -- evolution -- genetics -- growth, development and aging -- mathematics -- methodology -- mutation -- nanotechnology -- pH -- physiology -- population dynamics -- time -- Bacteria -- Bacterial Physiology -- Cell Culture Techniques -- Escherichia coli -- Evolution -- Hydrogen-Ion Concentration -- Mathematics -- Models, Biological -- Mutation -- Nanotechnology -- Population Dynamics -- Time Factors

Scopus
Держатели документа:
Krasnoyarsk State University, Krasnoyarsk, 660062, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Brilkov, A.V.; Loginov, I.A.; Morozova, E.V.; Pechurkin, N.S.

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17.

Chemiluminescence of mushrooms cultivated in Vietnam / D. T. Van, N. S. Manukovsky, I. I. Gitelson // Doklady Biochemistry and Biophysics. - 2013. - Vol. 448, Is. 1. - P13-14, DOI 10.1134/S1607672913010043 . - ISSN 1607-6729
Кл.слова (ненормированные):
Agaricales -- article -- chemistry -- growth, development and aging -- immunology -- luminescence -- species difference -- Viet Nam -- Agaricales -- Luminescence -- Species Specificity -- Vietnam

Scopus
Держатели документа:
Luminescent Biotechnology Co., LTD, Ho Chi Minh City, Viet Nam
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Svobodnyi pr. 79, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Van, D.T.; Manukovsky, N.S.; Gitelson, I.I.

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18.

Colony structure of a consortium of nitrifying bacteria / A. P. Puzyr' [и др.] // Mikrobiologiya. - 2001. - Vol. 70, Is. 1. - P. 98-105 . - ISSN 0026-3656
Кл.слова (ненормированные):
Colony architectonics -- Electron microscopy -- Nitrifying bacteria -- article -- bacterium -- bioreactor -- culture medium -- electron microscopy -- growth, development and aging -- nitrogen fixation -- ultrastructure -- Bacteria -- Bioreactors -- Culture Media -- Microscopy, Electron -- Nitrogen Fixation
Аннотация: Colonies produced by a consortium of nitrifying bacteria were studied using light and electron microscopy. The colonies were obtained by direct plating of inoculum from a two-stage nonsterile chemostat fermentor and by repeatedly passing the microbial community of the fermenter through selective media containing ammonium or nitrite. The colonies studied can be characterized by a specific combination of six types of cells differing in their ultrastructure and spatial location within the colony. The types of cells occurring within a given colony were found to depend on the nitrogen compound present in the medium. As a result of our study, morphological features of colonial bacterial communities were preliminarily identified. The proposed approach can be viewed as a method to describe microbial associations and communities.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Puzyr', A.P.; Mogil'Naya, O.A.; Gurevich, Y.L.; Babkina, E.A.

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19.

Long-term experiments on man's stay in biological life-support system / I. I. Gitelson [et al.] // Advances in Space Research. - 1989. - Vol. 9, Is. 8. - P65-71 . - ISSN 0273-1177
Кл.слова (ненормированные):
Agricultural Products -- Ecosystems -- Food Products -- Personnel - Health -- Space Flight - Manned Flight -- BIOS-3 Complex -- Phytotrons -- Spacecraft -- water -- air conditioning -- article -- biomass -- crop -- evaluation -- growth, development and aging -- human -- instrumentation -- light -- metabolism -- methodology -- microclimate -- photosynthesis -- plant -- waste management -- wheat -- Air Conditioning -- Biomass -- Crops, Agricultural -- Ecological Systems, Closed -- Evaluation Studies -- Humans -- Life Support Systems -- Light -- Photosynthesis -- Plants -- Triticum -- Waste Management -- Water
Аннотация: We describe the experimental system having maximal possible closure of material recycling in an ecosystem, including people and plants, which was carried out in a hermetically sealed experimental complex "BIOS-3", 315 m2 in volume. The system included 2 experimentators and 3 phytotrons with plants (total sowing area of 63 m2). Plants were grown with round-the-clock lamp irradiation with 130 Wm-2 PAR intensity. The plants production was food for people. Water exchange of ecosystem, as well as gas exchange, was fully closed excluding liquids and gas samples taken for chemical analysis outside the system. The total closure of material turnover constituted 91%. Health state of the crew was estimated before, during and after the experiment. A 5-months period did not affect their health. The experiments carried out prove that the closed ecosystem of "man-plants" is a prototype of a life-support system for long-term space expeditions. В© 1989.

Scopus
Держатели документа:
Institute of Biophysics, U.S.S.R. Academy of Sciences, Siberian Branch, Krasnoyarsk, 600366, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Terskov, I.A.; Kovrov, B.G.; Lisovskii, G.M.; Okladnikov, Yu.N.; Sid'ko, F.Ya.; Trubachev, I.N.; Shilenko, M.P.; Alekseev, S.S.; Pan'kova, I.M.; Tirranen, L.S.

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20.

Life support system with autonomous control employing plant photosynthesis / I. I. Gitelson [et al.] // Acta Astronautica. - 1976. - Vol. 3, Is. 9-10. - P633-650 . - ISSN 0094-5765
Кл.слова (ненормированные):
BIOCHEMICAL ENGINEERING - Photosynthesis -- SPACECRAFT -- adaptation -- article -- Chlorella -- growth, development and aging -- human -- instrumentation -- intestine -- male -- metabolism -- microbiology -- microclimate -- photosynthesis -- physiology -- plant -- space flight -- task performance -- vegetable -- water supply -- wheat -- Adaptation, Physiological -- Chlorella -- Ecological Systems, Closed -- Environment, Controlled -- Humans -- Intestines -- Life Support Systems -- Male -- Metabolism -- Photosynthesis -- Plants -- Space Simulation -- Task Performance and Analysis -- Triticum -- Vegetables -- Water Supply
Аннотация: This research was aimed at obtaining a closed control system. This was achieved by placing all the technological processes providing for human vital activities within the hermetically sealed space, and by transferring the entire control and guidance of these processes to people inhabiting the system. In contrast to existing biological life support systems, man has been included not only as a participant of metabolism, but as an operator who is the central figure in collecting information, making decisions and controlling all technological processes. To tackle this problem, the "BIOS-3" experimental complex was created for performing long-term experiments using different structures of biological life-support system. The experiment lasted six months and consisted of three stages. During the first stage the system was comprised of two equivalent phytotrons with the culture of wheat and an assortment of vegetable plants, and the living compartment. At the second stage, one of the phytotrons was removed while a compartment of chlorella cultivators was introduced. The third stage differed from the second, the former using wheat phytotron and the latter employing phytotron with an assortment of vegetable cultures. Three men inhabited the system simultaneously. The experiment demonstrated that a biological life support system controlled autonomously from the inside is feasible within a small confined space. However, immunological and microbiological research shows, that the medium created by the system is not fully adequate for man. In conclusion, some prospects have been outlined for further studies of biological life support systems. В© 1976.

Scopus
Держатели документа:
L. V. Kirensky Physics Institute, Siberian Branch, USSR Academy of Sciences, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Terskov, I.A.; Kovrov, B.G.; Sidko, F.Ya.; Lisovsky, G.M.; Okladnikov, Yu.N.; Belyanin, V.N.; Trubachov, I.N.; Rerberg, M.S.

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