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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson I.I., Terskov I.A., Kovrov B.G., Sidko F.Ya., Lisovsky G.M., Okladnikov Yu.N., Belyanin V.N., Trubachov I.N., Rerberg M.S.
Заглавие : Life support system with autonomous control employing plant photosynthesis
Место публикации : Acta Astronautica. - 1976. - Vol. 3, Is. 9-10. - С. 633-650. - ISSN 00945765 (ISSN)
Ключевые слова (''Своб.индексиров.''): biochemical engineering - photosynthesis--spacecraft--adaptation--article--chlorella--growth, development and aging--human--instrumentation--intestine--male--metabolism--microbiology--microclimate--photosynthesis--physiology--plant--space flight--task performance--vegetable--water supply--wheat--adaptation, physiological--chlorella--ecological systems, closed--environment, controlled--humans--intestines--life support systems--male--metabolism--photosynthesis--plants--space simulation--task performance and analysis--triticum--vegetables--water supply
Аннотация: This research was aimed at obtaining a closed control system. This was achieved by placing all the technological processes providing for human vital activities within the hermetically sealed space, and by transferring the entire control and guidance of these processes to people inhabiting the system. In contrast to existing biological life support systems, man has been included not only as a participant of metabolism, but as an operator who is the central figure in collecting information, making decisions and controlling all technological processes. To tackle this problem, the "BIOS-3" experimental complex was created for performing long-term experiments using different structures of biological life-support system. The experiment lasted six months and consisted of three stages. During the first stage the system was comprised of two equivalent phytotrons with the culture of wheat and an assortment of vegetable plants, and the living compartment. At the second stage, one of the phytotrons was removed while a compartment of chlorella cultivators was introduced. The third stage differed from the second, the former using wheat phytotron and the latter employing phytotron with an assortment of vegetable cultures. Three men inhabited the system simultaneously. The experiment demonstrated that a biological life support system controlled autonomously from the inside is feasible within a small confined space. However, immunological and microbiological research shows, that the medium created by the system is not fully adequate for man. In conclusion, some prospects have been outlined for further studies of biological life support systems. В© 1976.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson I.I., Terskov I.A., Kovrov B.G., Lisovskii G.M., Okladnikov Yu.N., Sid'ko F.Ya., Trubachev I.N., Shilenko M.P., Alekseev S.S., Pan'kova I.M., Tirranen L.S.
Заглавие : Long-term experiments on man's stay in biological life-support system
Место публикации : Advances in Space Research. - 1989. - Vol. 9, Is. 8. - С. 65-71. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): agricultural products--ecosystems--food products--personnel - health--space flight - manned flight--bios-3 complex--phytotrons--spacecraft--water--air conditioning--article--biomass--crop--evaluation--growth, development and aging--human--instrumentation--light--metabolism--methodology--microclimate--photosynthesis--plant--waste management--wheat--air conditioning--biomass--crops, agricultural--ecological systems, closed--evaluation studies--humans--life support systems--light--photosynthesis--plants--triticum--waste management--water
Аннотация: We describe the experimental system having maximal possible closure of material recycling in an ecosystem, including people and plants, which was carried out in a hermetically sealed experimental complex "BIOS-3", 315 m2 in volume. The system included 2 experimentators and 3 phytotrons with plants (total sowing area of 63 m2). Plants were grown with round-the-clock lamp irradiation with 130 Wm-2 PAR intensity. The plants production was food for people. Water exchange of ecosystem, as well as gas exchange, was fully closed excluding liquids and gas samples taken for chemical analysis outside the system. The total closure of material turnover constituted 91%. Health state of the crew was estimated before, during and after the experiment. A 5-months period did not affect their health. The experiments carried out prove that the closed ecosystem of "man-plants" is a prototype of a life-support system for long-term space expeditions. В© 1989.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rodicheva E.K., Trubachev I.N., Medvedeva S.E., Egorova O.I. U - Shitova LYu
Заглавие : Growth and luminescence of luminous bacteria promoted by agents of microbial origin.
Место публикации : Journal of bioluminescence and chemiluminescence. - 1993. - Vol. 8, Is. 6. - С. 293-299. - ISSN 08843996 (ISSN)
Ключевые слова (''Своб.индексиров.''): amino acid--carbohydrate--folic acid--luciferase--nitrogen--riboflavin--article--biosynthesis--culture medium--electron microscopy--growth, development and aging--kinetics--luminescence--metabolism--photobacterium--physiology--time--ultrastructure--vibrio--amino acids--carbohydrates--culture media--folic acid--kinetics--luciferase--luminescence--microscopy, electron--nitrogen--photobacterium--riboflavin--time factors--vibrio
Аннотация: The examination of four species of luminous bacteria Photobacterium leiognathi, Photobacterium phosphoreum, Vibrio fischeri and Vibrio harveyi has enabled us to reveal some nutrient medium components effecting growth, luminescence intensity and luciferase synthesis. These agents are nucleic components (nucleotides, nucleotides and amine bases), amino acids and vitamins, which are part of hydrolysates from the biomass of various lithotrophic microorganisms, hydrogen-oxidizing, iron-oxidizing and carboxydobacteria. The effect of promoting agents essentially alters the physiological state and ultrastructure of the cells of luminous bacteria and increases luciferase biosynthesis two- to three-fold compared to a control.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson J.I. U - Okladnikov YuN
Заглавие : Man as a component of a closed ecological life support system
Место публикации : Life support & biosphere science : international journal of earth space. - 1994. - Vol. 1, Is. 2. - С. 73-81. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon dioxide--oxygen--article--blood analysis--blood cell count--breathing--construction work and architectural phenomena--crop--diet--growth, development and aging--human--metabolism--microclimate--physiology--russian federation--space flight--standard--waste management--water supply--blood cell count--blood chemical analysis--carbon dioxide--crops, agricultural--diet--ecological systems, closed--environment, controlled--facility design and construction--humans--life support systems--oxygen--respiration--russia--space flight--waste management--water supply
Аннотация: Material support of all manned space flights so far has been provided from a prestored stock of substances or replenished from the Earth's biosphere. Exploration of space will, however, become real only when man is able to break away from Earth completely, when he will be accompanied by a system providing everything necessary to sustain full-valued life for an unlimited time. The only known system to date meeting this requirement is the Earth's biosphere. To break away from his cradle, as K.E. Tsiolkovsky called Earth, it is necessary to devise a life support system functionally similar to the natural biosphere. This need not be similar in structure to the vast diversity of trophic relationships available on Earth, but requires the solution of a multitude of various problems of an ecological, physiological, engineering and social-psychological nature. Human life-support systems based on biological regeneration of environments in small volumes have been studied at the Institute of Biophysics (Siberian Branch of the Russian Academy of Sciences) over many years. This work has resulted in the design of Bios-3, a biologically-based self-sustained human life support system.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson J.I., V B., Grigoriev A.I., Lisovsky G.M., Manukovsky N.S., Sinyak Y.u.E., Ushakova S.A.
Заглавие : Biological-physical-chemical aspects of a human life support system for a lunar base
Место публикации : Acta Astronautica. - 1995. - Vol. 37, Is. C. - С. 385-394. - ISSN 00945765 (ISSN)
Ключевые слова (''Своб.индексиров.''): animal--aquaculture--article--biomass--construction work and architectural phenomena--cyprinodontiformes--filtration--growth, development and aging--human--microbiology--microclimate--moon--nutritional value--photoperiodicity--plant--space flight--standard--tilapia--waste management--water management--wheat--animals--aquaculture--biomass--cyprinodontiformes--ecological systems, closed--facility design and construction--filtration--humans--life support systems--moon--nutritive value--photoperiod--plants, edible--space flight--tilapia--triticum--waste management--water microbiology--water purification
Аннотация: To create a life support system based on biological and physical-chemical processes is the optimum solution providing full-valued condidtions for existence and efficient work of people at a lunar base. Long-standing experinece in experimental research or closed ecosystems and their components allows us to suggest a realistic functional structure of the lunar base and to estimate qualitatively its parameters. The original restrictions are as follows: 1) the basic source of energy to support the biological processes has to be the solar radiation; 2) the initial amount of basic biological elelments forming the turnover of substances (C, O, H, P, K, N) has to be delivered from Earth; 3). Moon materials are not to be used in the biological turnover inside the base; 4) the base is to supply the crew fully with atmosphere and water, and with 90% (A scenario) or 40% (B scenario) of food. Experimental data about the plant productivity under the "Moon" rhythm of light and darkness allow us to suggest that the A scenario requires per one human: plant area - 40 m2 irradiated during the lunar day by 250-300 W/m2 PAR producing 1250 g of dry biomass a terrestrial day; a heterotrophic component of "biological incineration" of inedible plant biomass (800 g/day) including the aquaculture of fish to produce animal products and contaminating the environment less than birds and mammals, and the culture of edible mushrooms; a component of physical-chemical correction for the LSS envi ronment including the subsystems of: deep oxidation of organic impurities in the atmosphere and of water, organic wastes of human activity and that biological components (420 g/day) Co2 concentration in "Moon" nights, damping O2 in "Moon" days, etc. The stock of presotred or delivered from Earth substances (food additions, seeds, etc.) to be involved in biological turnover is to be about 50 kg/year per man. Increase of the mass of prestored substances per man up to 220 kg/year would reduce twice the plant area and consumed amount of radiant energy to exclude the components of "biological incineration" and physical-chemical destruction of organic wastes. В© 1995.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A.
Заглавие : Spectral composition of light and plant productivity
Место публикации : Advances in Space Research. - 1996. - Vol. 18, Is. 4-5. - С. 259-263. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--biology--cucumber--growth, development and aging--illumination--light--maize--photon--photosynthesis--plant--radiation exposure--spectroscopy--sunflower--tomato--wheat--cucumis sativus--helianthus--light--lighting--lycopersicon esculentum--photobiology--photons--photosynthesis--plants--spectrum analysis--triticum--zea mays
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Salisbury F.B., Gitelson J.I., Lisovsky G.M.
Заглавие : Bios-3: Siberian experiments in bioregenerative life support
Место публикации : BioScience. - 1997. - Vol. 47, Is. 9. - С. 575-585. - ISSN 00063568 (ISSN)
Ключевые слова (''Своб.индексиров.''): agriculture--chlorella--construction work and architectural phenomena--crop--energy metabolism--evaluation--growth, development and aging--human--metabolism--methodology--microbiology--microclimate--nasa discipline life support systems--non-nasa center--photon--review--russian federation--space flight--nasa discipline life support systems--non-nasa center--agriculture--chlorella--crops, agricultural--ecological systems, closed--energy metabolism--environment, controlled--environmental microbiology--evaluation studies--facility design and construction--humans--life support systems--photons--siberia--space flight--space simulation
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson J.I., Tirranen L.S., Borodina E.V., Rygalov V.Ye.
Заглавие : Impaired growth of plants cultivated in a closed system: Possible reasons
Место публикации : Advances in Space Research. - 1997. - Vol. 20, Is. 10. - С. 1927-1930. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): air conditioning--air pollutant--article--culture medium--growth, development and aging--human--hydroponics--methodology--microbiology--microclimate--plant--sewage--wheat--air conditioning--air pollutants--culture media--ecological systems, closed--environment, controlled--humans--hydroponics--life support systems--plants--triticum--waste disposal, fluid--water microbiology
Аннотация: Plants in experiments on "man-higher plants" closed ecosystem (CES) have been demonstrated to have inhibited growth and reduced productivity due to three basic factors: prolonged usage of a permanent nutrient solution introduction into the nutrient medium of intra-system gray water, and closure of the system. Gray water was detrimental to plants the longer the nutrient solution was used. However, higher plant growth was mostly affected by the gaseous composition of the CES atmosphere, through accumulation of volatile substances. В© 1997 COSPAR. Published by Elsevier Science Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A., Pisman T.I.
Заглавие : Catalase activity as a potential indicator of the reducer component of small closed ecosystems
Место публикации : Advances in Space Research. - 1997. - Vol. 20, Is. 10. - С. 1945-1948. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): carboxymethylcellulose--catalase--animal--article--bacillus--bacterial count--chlorella--culture medium--enzymology--growth, development and aging--metabolism--microclimate--paramecium--animals--bacillus--carboxymethylcellulose--catalase--chlorella--colony count, microbial--culture media--ecological systems, closed--paramecium
Аннотация: Dynamics of catalase activity has been shown to reflect the growth curve of microorganisms in batch cultivation (celluloselythic bacteria Bacillus acidocaldarius and bacteria of the associated microflora Chlorella vulgaris). Gas and substrate closure of the three component ecosystems with spatially separated components "producer-consumer-reducer" (Chl. vulgaris-Paramecium caudatum-B. acidocaldarius, two bacterial strains isolated from the associated microflora Chl. vulgaris) demonstrated that the functioning of the reducer component can be estimated by the catalase activity of microorganisms of this component. В© 1997 COSPAR. Published by Elsevier Science Ltd.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Somova L.A., Pechurkin N.S., Polonsky V.I., Pisman T.I., Sarangova A.B., Andre M., Sadovskaya G.M.
Заглавие : Plants-rhizospheric organisms interaction in a manmade system with and without biogenous element limitation
Место публикации : Advances in Space Research. - 1997. - Vol. 20, Is. 10. - С. 1939-1943. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): nitrogen--article--bacterial count--biomass--comparative study--culture medium--growth, development and aging--metabolism--microbiology--plant leaf--plant root--plant seed--pseudomonas fluorescens--pseudomonas putida--wheat--biomass--colony count, microbial--culture media--nitrogen--plant leaves--plant roots--pseudomonas fluorescens--pseudomonas putida--seeds--triticum
Аннотация: The effect has been studied of inoculation of seeds of wheat with two species of rhizospheric microorganisms, -Pseudomonas fluorescens and Pseudomonas putida - on young plant growth with complete and with nitrogen deficit mineral nutrition. With complete mineral medium, plants grown from seeds inoculated with bacteria of Pseudomonas genus (experiment plants) have been found to have better growth over plants not inoculated with these bacteria (control plants). The experiment plants had increased transpiration and their biomass had higher organic nitrogen content. With nitrogen deficit medium, the plants inoculated with bacteria and those without them, have not revealed changes in growth. Neither case demonstrated competition of microorganisms with plants for nitrogen sources. В© 1997 COSPAR. Published by Elsevier Science Ltd.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Zhilo N.O., Plotnikov V.F.
Заглавие : Physiological and biochemical properties of the alga Botryococcus braunii
Место публикации : Russian Journal of Plant Physiology. - 1998. - Vol. 45, Is. 6. - С. 775-779. - ISSN 10214437 (ISSN)
Ключевые слова (''Своб.индексиров.''): batch culture--botryococcus braunii--liquid hydrocarbons--productivity
Аннотация: The growth, productivity, and changes in the chemical composition of the green alga Botryococcus braunii Kutz H-252 were studied at different phases of growth in a luminostat culture. Modification of the Prat medium resulted in an increase in the alga yield to 3.9 g/l and a decrease in the generation time to 3-4 days. The specific growth rate during the exponential phase was 0.235/ day. These values are comparable with the best results obtained abroad. Among the pigments of B. braunii, besides chlorophylls a and b, carotene, lutein, neoxanthin, and violaxanthin were also identified. The highest content of these pigments, based on dry weight, was achieved during the exponential growth phase. During culture aging, there was an accumulation of carbohydrates and lipids in addition to a simultaneous decrease in the concentration of nitrogen-containing substances. It was discovered that this strain can synthesize liquid hydrocarbons. These hydrocarbons were found both in the alga biomass (ca. 1% of dry weight) and among the extracellular metabolites (up to 10.3 mg/l).
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pisman T.I., Pechurkin N.S., Mariasova T.S., Somova L.A., Sarangova A.B.
Заглавие : A mathematical model of "plants-microorganisms" interaction on complete mineral medium and under nitrogen limitation
Место публикации : Advances in Space Research. - 1999. - Vol. 24, Is. 3. - С. 383-387. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): nitrogen--ecological modeling--interspecific interaction--nutrient limitation--plant--rhizosphere--article--biological model--biomass--comparative study--culture medium--drug effect--growth, development and aging--mathematics--microbiology--plant root--pseudomonas fluorescens--sweating--wheat--biomass--culture media--mathematics--models, biological--nitrogen--plant roots--plant transpiration--pseudomonas fluorescens--triticum
Аннотация: A mathematical model concerning the interaction of plants and rhizospheric microorganisms on complete mineral medium and under nitrogen limitation has been constructed. The model takes into account the closeness of plants and microorganisms in terms of the matter released by the plant and consumed by the microorganisms. The effect of rhizospheric microorganisms on plant growth with normal carbon dioxide and complete mineral medium has been demonstrated. Plants interacting with microorganisms have a greater biomass than plants growing without microorganisms. Wheat growth stimulation by metabolites of rhizospheric microorganisms under laboratory conditions on artificial soil has been experimentally demonstrated (Pechurkin, 1997). Under nitrogen limitation , the biomass of plants, with or without microorganisms, is identical, and is substantially reduced as compared with the medium with standard nitrogen. В© 1999 COSPAR. Published by Elsevier Science Ltd.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pechurkin N.S., Brilkov A.V., Ganusov V.V., Kargatova T.V., Maksimova E.E., Popova L.Yu.
Заглавие : Modelling of genetically engineered microorganisms introduction in closed artificial microcosms
Место публикации : Advances in Space Research. - 1999. - Vol. 24, Is. 3. - С. 335-341. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(99)00320-8
Ключевые слова (''Своб.индексиров.''): aquatic environment--artificial ecosystem--ecological modeling--genetically modified organism--alga--animal--article--bacterial count--bacterial gene--biological model--biomass--escherichia coli--feasibility study--genetic engineering--genetics--growth, development and aging--microbiology--microclimate--photobacterium--plasmid--protozoon--time--yeast--algae--animals--biomass--colony count, microbial--ecological systems, closed--escherichia coli--feasibility studies--genes, bacterial--genetic engineering--models, biological--photobacterium--plasmids--protozoa--time factors--water microbiology--yeasts
Аннотация: The possibility of introducing genetically engineered microorganisms (GEM) into simple biotic cycles of laboratory water microcosms was investigated. The survival of the recombinant strain Escherichia coli Z905 (Ap(r), Lux+) in microcosms depends on the type of model ecosystems. During the absence of algae blooming in the model ecosystem, the part of plasmid-containing cells E.coli decreased fast, and the structure of the plasmid was also modified. In conditions of algae blooming (Ankistrodesmus sp.) an almost total maintenance of plasmid-containing cells was observed in E.coli population. A mathematics model of GEM's behavior in water ecosystems with different level of complexity has been formulated. Mechanisms causing the difference in luminescent exhibition of different species are discussed, and attempts are made to forecast the GEM's behavior in water ecosystems.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pis'man T.I., Pechurkin N.S., Sarangova A.B., Somova L.A.
Заглавие : Experimental models of small closed systems with spatially separated unicellular organism-based components.
Место публикации : Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 2. - С. 133-139. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon dioxide--nitrogen--animal--article--biomass--candida--chlorella--ecosystem--growth, development and aging--metabolism--microclimate--paramecium--photosynthesis--physiology--animals--biomass--candida--carbon dioxide--chlorella--ecological systems, closed--ecosystem--nitrogen--paramecium--photosynthesis
Аннотация: Experimental models of small biotic cycles of different degree of closure and complexity with spatially separated components based on unicellular organisms have been studied. Gas closure of components looped into "autotroph-heterotroph" (chlorella-yeast) system doubled the lifetime of the system (as opposed to individually cultivated components). Higher complexity of the heterotroph component consisting of two yeast species also increased the lifetime of the system through more complete utilization of the substrate by competing yeast species. The lifetime of gas and substrate closed "producer-consumer" trophic chain (chlorella-paramecia) increased to 7 months. In 60 days the components' numbers reached their steady state followed by more than 40 cycles of the medium. The role of a predator organism (protozoan) in nitrogen cycling was demonstrated; reproduction of protozoa correlated directly with their emission of nitrogen in the ammonia form that is most optimum for growth of chlorella.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Somova L.A., Pechurkin N.S., Sarangova A.B., Pisman T.I., Polonsky V.I., Sadovskay G.M.
Заглавие : Small artificial ecosystems: response to variation of environmental factors (CO2 enrichment).
Место публикации : Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - С. 215-220. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon--carbon dioxide--article--biomass--comparative study--drug effect--ecosystem--growth, development and aging--metabolism--microbiology--microclimate--photosynthesis--plant seed--pseudomonas--wheat--biomass--carbon--carbon dioxide--ecosystem--environment, controlled--photosynthesis--pseudomonas--seeds--soil microbiology--triticum
Аннотация: Response of "wheat plants--rhizospheric microorganisms--artificial soil"--a simple terrestrial ecosystem--to carbon dioxide increased in its atmosphere to 0.06% has been studied. It has been experimentally demonstrated that a simple ecosystem develops and functions different from its individual elements (components), in this case "plants-artificial soil" without microorganisms. With mineral nutrition unlimited and CO2 enrichment the system is capable of binding (involving into turnover) 40% more carbon than the system without microorganisms. With material balance as the basis, this article evaluates the contribution of a system's elements into its development, namely, the contribution of the photosynthesizing component and the contribution of microorganisms.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ganusov V.V., Bril'kov A.V., Pechurkin N.S.
Заглавие : Mathematical modeling of population dynamics of unstable plasmid-containing bacteria during continuous cultivation in a chemostat
Место публикации : Biofizika. - 2000. - Vol. 45, Is. 5. - С. 908-914. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--bioreactor--escherichia coli--fermentation--genetics--growth, development and aging--photobacterium--plasmid--theoretical model--bioreactors--escherichia coli--fermentation--models, theoretical--photobacterium--plasmids
Аннотация: A structural approach to studying the regularities of the population dynamics of unstable recombinant bacterial strains in a chemostat was elaborated. The approach is based on the mathematical modeling of cell distribution in a population with different numbers of plasmid copies. The effect of decreased selective preference of plasmidless variants of the recombinant strain in the chemostat, which is related to a decrease in the number of plasmid copies in cells upon long-term incubation was analyzed. It is shown that the time of half-elimination of plasmids from the bacterial population in the steady state in the chemostat T1/2 does not depend on the maximum number of plasmid copies in cells N but is determined only by the mean time of generation g and the probability of the loss of one plasmid copy tau. The dependence of the preference of bacterial plasmidless variants on the efficiency of expression of genes cloned into plasmids in chemostat was analyzed using the recombinant strain E. coli Z905, whose plasmids pPHL-7 contain cloned genes for the luminescence system of marine luminescing bacteria Photobacterium leiognathi.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Kolmakov V.I., Kravchuk E.S., Ivanova E.A., Trusova M.Y., Gribovskaya I.V., Zhilenkov M.D.
Заглавие : Growth and survival of cyanobacteria in water from blooming and nonblooming ponds under experimental conditions.
Место публикации : Doklady Biological Sciences. - 2000. - Vol. 375. - С. 607-609. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): chlorophyll--fresh water--phosphorus--anabaena--arctic--article--biomass--comparative study--cyanobacterium--growth, development and aging--microbiology--ph--plant--russian federation--season--anabaena--arctic regions--biomass--chlorophyll--cyanobacteria--fresh water--hydrogen-ion concentration--phosphorus--plants--russia--seasons
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsk A.Ya., Kosinenko S.V.
Заглавие : Intracellular phosphorus pool of the cyanobacterium spirulina platensis
Место публикации : Mikrobiologiya. - 2000. - Vol. 69, Is. 1. - С. 135-137. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): phosphorus--article--biomass--cyanobacterium--growth, development and aging--metabolism--biomass--cyanobacteria--phosphorus
Аннотация: An intracellular phosphorus pool in a monoculture of the cyanobacterium Spirulina platensis was assessed using radioactive and nonradioactive phosphorus. The derived dependence of specific growth rate on the intracellular content of mineral phosphorus can be presented in the form of the Droop equation. It was found that the stage of replenishment of the intracellular phosphorus pool may affect the phosphorus turnover estimation in aquatic environments from the results of short-term measurements of phosphorus uptake.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Kolmakov V.I., Dubovskaya O.P., Ivanova E.A.
Заглавие : The microalgal food spectrum of Daphnia longispina during the algal bloom of an eutrophic water body.
Место публикации : Doklady Biological Sciences. - 2000. - Vol. 371, Is. 1-6. - С. 179-181. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): alga--animal--article--biomass--daphnia--ecosystem--food chain--growth, development and aging--microbiology--plankton--zooplankton--algae--animals--biomass--daphnia--ecosystem--food chain--plankton--water microbiology--zooplankton
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Yu., Lobova T.I., Krylova T.Yu., Kargatova T.V., Maksimova E.E., Boyandin A.N., Pechurkin N.S.
Заглавие : Population dynamics of transgenic microorganisms in the different microecosystem conditions
Место публикации : Advances in Space Research. - 2001. - Vol. 27, Is. 9. - С. 1571-1579. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(01)00248-4
Ключевые слова (''Своб.индексиров.''): ecosystems--escherichia coli--genes--interferons--luminescence--vaccines--microcosms--microorganisms--fresh water--microorganism--adaptation--article--bacillus subtilis--comparative study--ecosystem--escherichia coli--gene expression--genetics--growth, development and aging--hazardous waste--microbiology--micrococcus--osmotic pressure--plasmid--population dynamics--risk assessment--transgenic organism--adaptation, physiological--bacillus subtilis--containment of biohazards--ecosystem--escherichia coli--fresh water--gene expression--micrococcus--organisms, genetically modified--osmotic pressure--plasmids--population dynamics--risk assessment--water microbiology
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap1Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human ? 2-interferon Km1Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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