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Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

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Отечественные журналы библиотеки Института биофизики СО РАН

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Труды сотрудников ИБФ СО РАН

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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bashmakova, Eugenia E., Panamarev, Nikita S., Kudryavtsev, Alexander N., Frank, Ludmila A.
Заглавие : N-extended photoprotein obelin to competitively detect small protein tumor markers
Колич.характеристики :5 с
Коллективы : Russian Federation for young scientists [MK-772.2020.4]; Government of Krasnoyarsk Territory, Krasnoyarsk Regional Science Foundation [2021012006966]
Место публикации : Biochem. Biophys. Res. Commun.: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2022. - Vol. 598. - С. 69-73. - ISSN 0006-291X, DOI 10.1016/j.bbrc.2022.02.011. - ISSN 1090-2104(eISSN)
Примечания : Cited References:15. - The work was partially supported by a grant of the President of the Russian Federation for young scientists, the candidates of sciences (project MK-772.2020.4, study of a hybrid protein with melanoma-inhibiting activity) and Government of Krasnoyarsk Territory, Krasnoyarsk Regional Science Foundation (project No 2021012006966, study of a hybrid with protein survivin).
Предметные рубрики: MELANOMA INHIBITORY-ACTIVITY
SURVIVIN
Аннотация: Two variants of Ca2+-regulated photoprotein obelin, extended from the N-terminus with small tumor markers-melanoma inhibitory activity protein (MIA) and survivin, one of the protein inhibitors of apoptosis, were designed, obtained and studied. Both domains in the obtained hybrid proteins exhibit the properties of the initial molecules: the main features of Ca2+-triggered bioluminescence are close to those of obelin, and the tumor markers' domains are recognized and bound by the corresponding antibodies. The obtained hybrids compete with the corresponding tumor markers for binding with antibodies, immobilized on the surface and their use has been shown to be promising as bioluminescent labels in a one-stage solid-phase competitive immunoassay. (c) 2022 Published by Elsevier Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lagutkin D., Semyonov D., Yegorov Y. E., Lavrinenko I., Generalov E., Zaitceva A. Y., Matveeva O., Nechipurenko Y. D.
Заглавие : Positive feedback between acidosis and hypoxia during the transition of COVID-19 to a severe form of the disease
Колич.характеристики :1 с
Место публикации : FEBS Open Bio: WILEY, 2021. - Vol. 11. - С. 450-450. - ISSN 2211-5463
Примечания : Cited References:0
Аннотация: SARSCoV2 virus causes disease that has several distinctive characteristics compared to diseases caused by other viruses. We have put forward a hypothesis that relates COVID19 pathogenesis with acidosis, which frequently characterizes severe cases of this disease. It has been shown that hypoxia and acidosis affect the progression of severe COVID19 at various physiological levels such as organs, tissues, and cells. The physiological effects of acidosis and hypoxia range from the level of compensatory capabilities of the whole organism to the functioning of a single hemoglobin molecule. In our work, we consider several mechanisms that link the damaging factors of COVID19 with acidosis. These mechanisms reveal stepbystep processes with a pronounced positive feedback. In accordance with the well known Bohr effect, a decrease in blood pH leads to a drop in blood oxygen saturation. At the same time, this drop in saturation contributes to the further development of acidosis. This indicates a depletion of the body's compensatory capabilities to regulate acidosis and leads to deterioration of the patient's condition. In addition, a decrease in pH can cause conformational changes in the viral Sprotein, followed by changes in ability of some antibodies to recognize the virus. This might lead to the decrease in antibodies affinity and avidity, negatively affecting virus clearance. Low levels of pH and hypoxia in blood and tissues can induce a proinflammatory innate response even in the absence of antigen stimulation. Therefore, hypoxia and acidosis can lead to a restructuring of the immune system and multidirectional pro and antiinflammatory responses, which often, instead of recovery, lead to the disease aggravation.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nechipurenko Y. D., Semyonov D. A., Lavrinenko I. A., Lagutkin D. A., Generalov E. A., Zaitceva A. Y., Matveeva O. V., Yegorov Y. E.
Заглавие : The role of acidosis in the pathogenesis of severe forms of COVID-19
Место публикации : Biology: MDPI, 2021. - Vol. 10, Is. 9. - Ст.852. - ISSN 20797737 (ISSN), DOI 10.3390/biology10090852
Аннотация: COVID-19 has specific characteristics that distinguish this disease from many other infec-tions. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient’s condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregu-lation of the immune system and multidirectional pro-and anti-inflammatory reactions, resulting in the development of a “cytokine storm”. In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nechipurenko, Yury D., Semyonov, Denis A., Lavrinenko, Igor A., Lagutkin, Denis A., Generalov, Evgenii A., Zaitceva, Anna Y., Matveeva, Olga, V, Yegorov, Yegor E.
Заглавие : The Role of Acidosis in the Pathogenesis of Severe Forms of COVID-19
Колич.характеристики :15 с
Коллективы : Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology; Program of Fundamental Research for State Academies for years 2013-2020 [01201363818]
Место публикации : Biology-Basel: MDPI, 2021. - Vol. 10, Is. 9. - Ст.852. - ISSN 2079-7737(eISSN), DOI 10.3390/biology10090852
Примечания : Cited References:86. - This research was funded by the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology and the Program of Fundamental Research for State Academies for years 2013-2020, project no. 01201363818.
Предметные рубрики: RESPIRATORY-ACIDOSIS
LACTATE
COAGULATION
GLYCOLYSIS
SECRETION
Аннотация: Simple Summary Recently, several studies have shown that acidosis, which is increased acidity in the blood and other body tissues, is often associated with severe COVID-19. In this article, we look at the mechanisms and consequences of acidosis that can lead to an exacerbation of COVID-19. We want to draw the attention of readers to the threshold values of such disease characteristics as hypoxia and acidosis, which are associated with a sharp deterioration in the patient's condition. Hypoxia and acidosis mutually reinforce each other according to the principle of a vicious cycle (that is, they are involved in a system of positive feedbacks). Elevated blood lactate (lactic acid) levels are associated with poor clinical outcomes in COVID patients. As a practical recommendation, we propose to pay more attention to the prevention of acidosis, including in the early stages of the disease, when the adjustment of homeostasis requires less effort and is less risky. COVID-19 has specific characteristics that distinguish this disease from many other infections. We suggest that the pathogenesis of severe forms of COVID-19 can be associated with acidosis. This review article discusses several mechanisms potentially linking the damaging effects of COVID-19 with acidosis and shows the existence of a vicious cycle between the development of hypoxia and acidosis in COVID-19 patients. At the early stages of the disease, inflammation, difficulty in gas exchange in the lungs and thrombosis collectively contribute to the onset of acidosis. In accordance with the Verigo-Bohr effect, a decrease in blood pH leads to a decrease in oxygen saturation, which contributes to the exacerbation of acidosis and results in a deterioration of the patient's condition. A decrease in pH can also cause conformational changes in the S-protein of the virus and thus lead to a decrease in the affinity and avidity of protective antibodies. Hypoxia and acidosis lead to dysregulation of the immune system and multidirectional pro- and anti-inflammatory reactions, resulting in the development of a "cytokine storm". In this review, we highlight the potential importance of supporting normal blood pH as an approach to COVID-19 therapy.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chang, Dao, Liu, Yangkun, Chen, Yangyang, Hu, Xiaomin, Burov, Andrey, Puzyr, Alexey, Bondar, Vladimir, Yao, Lunguang
Заглавие : Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system
Колич.характеристики :9 с
Коллективы : National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31870917]; program for Innovative Research Team of Science and Technology in University of Henan Province [20IRTSTHN024]; Key Scientific Research Projects of Colleges and Universities in Henan Province of China [18B230008]
Место публикации : BMC Vet. Res.: BMC, 2020. - Vol. 16, Is. 1. - Ст.202. - ISSN 1746-6148(eISSN), DOI 10.1186/s12917-020-02422-3
Примечания : Cited References:30. - This work was financially supported by the National Natural Science Foundation of China (No. 31870917), The program for Innovative Research Team of Science and Technology in University of Henan Province (No. 20IRTSTHN024) and Key Scientific Research Projects of Colleges and Universities in Henan Province of China (No. 18B230008). The funding bodies played no role in the design of the study, the collection, analysis, and interpretation of data and in writing the manuscript.
Предметные рубрики: VIRUS-LIKE PARTICLES
ESCHERICHIA-COLI
GENETIC-ANALYSIS
CPV-VP2
Аннотация: Background Canine parvovirus (CPV) is now recognized as a serious threat to the dog breeding industry worldwide. Currently used CPV vaccines all have their specific drawbacks, prompting a search for alternative safe and effective vaccination strategies such as subunit vaccine. VP2 protein is the major antigen targeted for developing CPV subunit vaccine, however, its production in baculovirus expression system remains challenging due to the insufficient yield. Therefore, our study aims to increase the VP2 protein production by using an improved baculovirus expression system and to evaluate the immunogenicity of the purified VP2 protein in mice. Results The results showed that high-level expression of the full length VP2 protein was achieved using our modified baculovirus expression system. The recombinant virus carrying two copies of VP2 gene showed the highest expression level, with a productivity of 186 mg/L, which is about 1.4-1.6 fold that of the recombinant viruses carrying only one copy. The purified protein reacted with Mouse anti-His tag monoclonal antibody and Rabbit anti-VP2 polyclonal antibody. BALB/c mice were intramuscularly immunized with purified VP2 protein twice at 2 week intervals. After vaccination, VP2 protein could induce the mice produce high level of hemagglutination inhibition antibodies. Conclusions Full length CPV VP2 protein was expressed at high level and purified efficiently. Moreover, it stimulated mice to produce high level of antibodies with hemmaglutination inhibition properties. The VP2 protein expressed in this study could be used as a putative economic and efficient subunit vaccine against CPV infection.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya V. V., Bashmakova E. E., Kudryavtsev A. N., Vorobjeva M. A., Shatunova E. A., Frank L. A.
Заглавие : The Hybrid Protein ZZ-OL as an Analytical Tool for Biotechnology Research
Колич.характеристики :7 с
Коллективы : Russian FederationRussian Federation [MK-772.2020.4]; Russian Science FoundationRussian Science Foundation (RSF) [16-14-10296]
Место публикации : Russ. J. Bioorg. Chem.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2020. - Vol. 46, Is. 6. - С. 1004-1010. - ISSN 1068-1620, DOI 10.1134/S106816202006014X. - ISSN 1608-330X(eISSN)
Примечания : Cited References:14. - The study was partially supported by a grant of the President of the Russian Federation for Young Scientists, the Candidates of Sciences (project MK-772.2020.4 in the part involving the synthesis and analysis of variants of proteins with melanoma-inhibiting activity) and a grant of the Russian Science Foundation (project no. 16-14-10296 in the part involving the bioluminescence analysis of binding of DNA aptamers to targets).
Аннотация: The gene of the hybrid protein that encodes the double synthetic fragment proZZ of the immunoglobulin-binding domain of protein A of Staphylococcus aureus and apo-obelin joined by a short linker has been cloned. The corresponding hybrid protein has been obtained by expression in Escherichia coli cells. The protein activated with a substrate (coelenterazine) possesses the bioluminescent Ca2+-dependent activity of the photoprotein close to that of recombinant wild-type obelin, and the immunoglobulin-binding ability of protein A. It has been shown that the hybrid can be used as a highly sensitive label to detect antibodies and estimate their affinity and interaction with recombinant proteins, as well as in investigations of other kinds.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chang D., Liu Y., Chen Y., Hu X., Burov A., Puzyr A., Bondar V., Yao L.
Заглавие : Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system
Место публикации : BMC Vet. Res.: BioMed Central Ltd., 2020. - Vol. 16, Is. 1. - Ст.202. - ISSN 17466148 (ISSN), DOI 10.1186/s12917-020-02422-3
Аннотация: Background: Canine parvovirus (CPV) is now recognized as a serious threat to the dog breeding industry worldwide. Currently used CPV vaccines all have their specific drawbacks, prompting a search for alternative safe and effective vaccination strategies such as subunit vaccine. VP2 protein is the major antigen targeted for developing CPV subunit vaccine, however, its production in baculovirus expression system remains challenging due to the insufficient yield. Therefore, our study aims to increase the VP2 protein production by using an improved baculovirus expression system and to evaluate the immunogenicity of the purified VP2 protein in mice. Results: The results showed that high-level expression of the full length VP2 protein was achieved using our modified baculovirus expression system. The recombinant virus carrying two copies of VP2 gene showed the highest expression level, with a productivity of 186 mg/L, which is about 1.4-1.6 fold that of the recombinant viruses carrying only one copy. The purified protein reacted with Mouse anti-His tag monoclonal antibody and Rabbit anti-VP2 polyclonal antibody. BALB/c mice were intramuscularly immunized with purified VP2 protein twice at 2 week intervals. After vaccination, VP2 protein could induce the mice produce high level of hemagglutination inhibition antibodies. Conclusions: Full length CPV VP2 protein was expressed at high level and purified efficiently. Moreover, it stimulated mice to produce high level of antibodies with hemmaglutination inhibition properties. The VP2 protein expressed in this study could be used as a putative economic and efficient subunit vaccine against CPV infection. © 2020 The Author(s).
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya, Vasilisa V., Chaukina, Valentina V., Abroskina, Maria V., Vorobyeva, Maria A., Ilminskaya, Aleksandra A., Kabilov, Marsel R., Prokopenko, Semyon V., Nevinsky, Georgy A., Venyaminova, Alya G., Frank, Ludmila A.
Заглавие : Bioluminescent aptamer-based sandwich-type assay of anti-myelin basic protein autoantibodies associated with multiple sclerosis
Колич.характеристики :7 с
Коллективы : Russian Foundation for Basic Research (RFBR), Russia [17-315-50027]; Russian State [AAAA-A17-117013050026-4, AAAA-A17-117020210021-7]
Место публикации : Anal. Chim. Acta: ELSEVIER SCIENCE BV, 2019. - Vol. 1064. - С. 112-118. - ISSN 0003-2670, DOI 10.1016/j.aca.2019.03.015. - ISSN 1873-4324(eISSN)
Примечания : Cited References:29. - This work was supported by the Russian Foundation for Basic Research (RFBR), Russia, under the grant No 17-315-50027; Russian State funded budget projects No. AAAA-A17-117013050026-4 and AAAA-A17-117020210021-7.
Предметные рубрики: ANTIBODIES
BIOMARKERS
RNA
Ключевые слова (''Своб.индексиров.''): bioluminescent microassay--rna aptamers--autoantibodies to myelin basic--protein--multiple sclerosis
Аннотация: Bioluminescent solid-phase sandwich-type microassay was developed to detect multiple sclerosis (MS)-associated autoantibodies in human sera. The assay is based on two different 2'-F-Py RNA aptamers against the target autoantibodies as biospecific elements, and Ca2+-regulated photoprotein obelin as a reporter. The paper describes elaboration of the assay and its application to 91 serum samples from patients with clinically definite MS and 86 ones from individuals healthy in terms of MS. Based on the receiver-operator curve (ROC) analysis, the chosen threshold value as clinical decision limit offers sensitivity of 63.7% and specificity of 94.2%. The area under the ROC curve (AUC) value of 0.87 shows a good difference between the groups under investigation. The likelihood ratio of 10.97 proves the diagnostic value of the assay and its potential as one of the laboratory MS-tests. (C) 2019 Elsevier B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Davydova, Anna, Vorobyeva, Mariya, Bashmakova, Eugenia, Vorobjev, Pavel, Krasheninina, Olga, Tupikin, Alexey, Kabilov, Marsel, Krasitskaya, Vasilisa, Frank, Ludmila, Venyaminova, Alya
Заглавие : Development and characterization of novel 2 '-F-RNA aptamers specific to human total and glycated hemoglobins
Колич.характеристики :8 с
Коллективы : Russian Science Foundation [16-14-10296]
Место публикации : Anal. Biochem.: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2019. - Vol. 570. - С. 43-50. - ISSN 0003-2697, DOI 10.1016/j.ab.2019.02.004. - ISSN 1096-0309(eISSN)
Примечания : Cited References:32. - We want to thank Dr. Alexander Lomzov (ICBFM SB RAS) for his valuable assistance with circular dichroism studies. The work was supported by the Russian Science Foundation (grant number 16-14-10296).
Предметные рубрики: RNA APTAMER
SEQUENCES
PROTEIN
DNA
Аннотация: Aptamers are short DNA and RNA fragments which bind their molecular targets with affinity and specificity comparable to those of antibodies. Here, we describe the selection of novel 2'-F-RNA aptamers against total human hemoglobin or its glycated form HbA1c. After SELEX and high-throughput sequencing of the enriched libraries, affinities and specificities of candidate aptamers and their truncated variants were examined by the solid-phase bioluminescent assay. As a result, we identified aptamers specific to both hemoglobins or only glycated HbA1c. The developed 2'-F-RNA aptamers have shown their applicability for detection of total and glycated hemoglobin in one sample.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ranjan R., Esimbekova E. N., Kratasyuk V. A.
Заглавие : Rapid biosensing tools for cancer biomarkers
Место публикации : Biosens. Bioelectron.: Elsevier Ltd, 2017. - Vol. 87. - С. 918-930. - ISSN 09565663 (ISSN) , DOI 10.1016/j.bios.2016.09.061
Ключевые слова (''Своб.индексиров.''): biosensor--cancer biomarker--functional nanomaterials--microfluidics--point-of-care devices--antibodies--biosensors--diseases--electronic properties--microfluidics--nanostructured materials--cancer biomarkers--diagnostic systems--fluorescent probes--functional nano materials--latest development--optoelectronic properties--point of care--rapid bio-sensing--biomarkers
Аннотация: The present review critically discusses the latest developments in the field of smart diagnostic systems for cancer biomarkers. A wide coverage of recent biosensing approaches involving aptamers, enzymes, DNA probes, fluorescent probes, interacting proteins and antibodies in vicinity to transducers such as electrochemical, optical and piezoelectric is presented. Recent advanced developments in biosensing approaches for cancer biomarker owes much credit to functionalized nanomaterials due to their unique opto-electronic properties and enhanced surface to volume ratio. Biosensing methods for a plenty of cancer biomarkers has been summarized emphasizing the key principles involved. © 2016 Elsevier B.V.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ranjan, Rajeev, Esimbekova, Elena N., Kratasyuk, Valentina A.
Заглавие : Rapid biosensing tools for cancer biomarkers
Колич.характеристики :13 с
Коллективы : Russian Foundation for Basic Research [16-34-60100, 16-06-00439]; state budget allocated to the fundamental research Russian Academy of Sciences Project [01201351504]
Место публикации : Biosens. Bioelectron.: ELSEVIER ADVANCED TECHNOLOGY, 2017. - Vol. 87. - С. 918-930. - ISSN 0956-5663, DOI 10.1016/j.bios.2016.09.061. - ISSN 1873-4235(eISSN)
Примечания : Cited References:115. - The research was partially supported by the Russian Foundation for Basic Research (Project no. 16-34-60100 and No. 16-06-00439), the state budget allocated to the fundamental research Russian Academy of Sciences (Project no. 01201351504).
Предметные рубрики: POINT-OF-CARE
HIGHLY SENSITIVE DETECTION
ACOUSTIC-WAVE BIOSENSOR
DNA
Ключевые слова (''Своб.индексиров.''): biosensor--cancer biomarker--functional nanomaterials--point-of-care--devices--microfluidics
Аннотация: The present review critically discusses the latest developments in the field of smart diagnostic systems for cancer biomarkers. A wide coverage of recent biosensing approaches involving aptamers, enzymes, DNA probes, fluorescent probes, interacting proteins and antibodies in vicinity to transducers such as electrochemical, optical and piezoelectric is presented. Recent advanced developments in biosensing approaches for cancer biomarker owes much credit to functionalized nanomaterials due to their unique opto-electronic properties and enhanced surface to volume ratio. Biosensing methods for a plenty of cancer biomarkers has been summarized emphasizing the key principles involved.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vorobjeva M.A., Krasitskaya V.V., Fokina A.A., Timoshenko V.V., Nevinsky G.A., Venyaminova A.G., Frank L.A.
Заглавие : RNA aptamer against autoantibodies associated with multiple sclerosis and bioluminescent detection probe on its basis
Место публикации : Anal. Chem. - 2014. - Vol. 86, Is. 5. - С. 2590-2594. - ISSN 00032700 (ISSN) , DOI 10.1021/ac4037894
Ключевые слова (''Своб.индексиров.''): autoantibodies--detection probes--developed model--high affinity--laboratory test--microplate assay--multiple sclerosis--myelin basic protein--antibodies--rna--bioluminescence
Аннотация: Nowadays, there are no specific laboratory tests for establishing the diagnosis of multiple sclerosis (MS). The presence of proteolytic autoantibodies against myelin basic protein is now considered as a characteristic feature of MS. New 2?-F-containing RNA aptamer of high affinity and specificity to these antibodies was selected. Covalent conjugate of this aptamer and Ca 2+-regulated photoprotein obelin was obtained for the first time and applied as a label in bioluminescent microplate assay to detect target antibodies. The developed model solid-phase microassay is simple, fast, and highly sensitive. © 2014 American Chemical Society.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O.A., Kiselyova E.V., Medvedeva S.E., Puzir A.P., Guseynov O.A., Kulyba N.N., Kozlov A.V.
Заглавие : Immunoelectronmicroscopic study of the nucleoid structure of hydrogen bacteria.
Место публикации : Journal of Basic Microbiology. - 1992. - Vol. 32, Is. 6. - С. 381-387. - ISSN 0233111X (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterial dna--alcaligenes--article--cell division--cell nucleus--escherichia coli--immunoelectron microscopy--ultrastructure--alcaligenes--cell division--cell nucleus--dna, bacterial--escherichia coli--microscopy, immunoelectron
Аннотация: Electron microscopical studies of the nucleoid structure of hydrogen bacteria using ultrahin sections and spread DNA from bacterial cell lysates revealed a different DNA packaging in the cell. A compact state of the major part of DNA at all growth stages and stability of nucleosome-like structures were shown. The use of antibodies to HU protein of E. coli labelled by protein A-colloidal gold demonstrated the immunological relationship between HU protein of E. coli and histone-like proteins of Alcaligenes eutrophus and their possible role in the nucleosome-like DNA packaging in procariotic genome.
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