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A study on the possibility of environmental adaptation of a Bacillus subtilis strain containing a recombinant plasmid with the gene of human interferon alpha 2 [Text] / L. Y. Popova [et al.] // Microbiology. - 1997. - Vol. 66, Is. 6. - P. 637-641. - Cited References: 13 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
GENETICALLY-MODIFIED MICROORGANISMS
Кл.слова (ненормированные):
microecosystem -- Bacillus subtilis -- recombinant plasmid -- interferon -- adaptation
Аннотация: Adaptation of the Bacillus subtilis strain 2335/105 (Km(r)Inf(+)) containing a recombinant plasmid encoding the extracellular human interferon alpha 2 was studied under various conditions. Stability of the plasmid in the population of B. subtilis 2335/105 was estimated under nonselective conditions. The plasmid-free cells and cells with a low number of plasmid copies were found to accumulate progressively, constituting 80% of the population after 10 culture passages, indicating the poor competitiveness of cells carrying a high number of plasmid copies. The behavior of vegetative cells of the recombinant strain introduced into aquatic microcosms differing in trophic chain length was studied. Within the first 10 days, the lysis of vegetative cells of B. subtilis 2335/105 occurred; the number of viable spores was very low but remained constant for half a year.

WOS
Держатели документа:
Russian Acad Sci, Akademgorodok, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
State Sci Ctr VB Vektor, Sci Res Design & Technol Inst Biol Act Subst, Berdsk 633190, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Y.; Kargatova, T.V.; Maksimova, E.E.; Belyavskaya, V.A.

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Catalase activity as a potential indicator of the reducer component of small closed ecosystems / A. B. Sarangova, L. A. Somova, T. I. Pisman // Advances in Space Research. - 1997. - Vol. 20, Is. 10. - P1945-1948 . - ISSN 0273-1177
Кл.слова (ненормированные):
carboxymethylcellulose -- catalase -- animal -- article -- Bacillus -- bacterial count -- Chlorella -- culture medium -- enzymology -- growth, development and aging -- metabolism -- microclimate -- Paramecium -- Animals -- Bacillus -- Carboxymethylcellulose -- Catalase -- Chlorella -- Colony Count, Microbial -- Culture Media -- Ecological Systems, Closed -- Paramecium
Аннотация: Dynamics of catalase activity has been shown to reflect the growth curve of microorganisms in batch cultivation (celluloselythic bacteria Bacillus acidocaldarius and bacteria of the associated microflora Chlorella vulgaris). Gas and substrate closure of the three component ecosystems with spatially separated components "producer-consumer-reducer" (Chl. vulgaris-Paramecium caudatum-B. acidocaldarius, two bacterial strains isolated from the associated microflora Chl. vulgaris) demonstrated that the functioning of the reducer component can be estimated by the catalase activity of microorganisms of this component. В© 1997 COSPAR. Published by Elsevier Science Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sarangova, A.B.; Somova, L.A.; Pisman, T.I.

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Catalase activity as a potential indicator of the reducer component of small closed ecosystems [Text] / A. B. Sarangova, L. A. Somova, T. I. Pisman ; ed. RM Wheeler [et al.] // LIFE SCIENCES: LIFE SUPPORT SYSTEMS STUDIES-I. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON PRESS LTD, 1997. - Vol. 20: F4.6, F4.8, F4.2 and F4.9 Symposia of COSPAR Scientific Commission F on Life Sciences - Life Support System Studies-I, at the 31st COSPAR Scientific Assembly (JUL 14-SEP 21, 1996, BIRMINGHAM, ENGLAND), Is. 10. - P. 1945-1948, DOI 10.1016/S0273-1177(97)00630-3. - Cited References: 8 . - ISBN 0273-1177. - ISBN 0-08-043307-3

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: Dynamics of catalase activity has been shown to reflect the growth curve of microorganisms in batch cultivation (celluloselythic bacteria Bacillus acidocaldarius and bacteria of the associated microflora Chlorella vulgaris). Gas and substrate closure of the three component ecosystems with spatially separated components "producer-consumer-reducer" (Chl, vulgaris-Paramecium caudatum-B. acidocaldarius, two bacterial strains isolated from the associated microflora Chl. vulgaris) demonstrated that the functioning of the reducer component can be estimated by the catalase activity of microorganisms of this component. (C) 1997 COSPAR. Published by Elsevier Science Ltd.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sarangova, A.B.; Somova, L.A.; Pisman, T.I.; Wheeler, RM \ed.\; Garland, JL \ed.\; Tibbitts, TW \ed.\; Nielsen, SS \ed.\

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Architectonics of a mixed colony Flavobacterium sp 56+Bacillus cereus 60 [Текст] / A. P. Puzyr, O. A. Mogil'naya, E. L. Pavlyuchenko // Izv. Akad. Nauk Ser. Biol. - 1999. - Is. 3. - P. 378-384. - Cited References: 15 . - ISSN 0002-3329

РУБ Biology

Аннотация: Electron microscopy has shown that the colonies obtained as a result of joint growth of bacterial monocultures Flavobacterium sp. 56 and Bacillus cereus 60 consist of three main cell types. Two cell types of the mixed colonies preserve the fine structure specific for bacteria in the monocultures. At the same time, formation of a large amount of the third type of cells is stimulated, which occur as single cells in the monoculture Flavobacterium sp. 56. The cell types are not intermixed in the colony volume and have certain spatial positions.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Puzyr, A.P.; Mogil'naya, O.A.; Pavlyuchenko, E.L.

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Effect of salinity on the adaptive capacity of recombinant strains of escherichia coli and bacillus subtilis / A. N. Boyandin [и др.] // Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 243-247 . - ISSN 0026-3656
Кл.слова (ненормированные):
Genetically modified microorganisms -- Salinity -- Salt resistance -- sodium chloride -- adaptation -- article -- Bacillus subtilis -- electrolyte balance -- Escherichia coli -- genetic recombination -- genetics -- physiology -- Adaptation, Biological -- Bacillus subtilis -- Escherichia coli -- Recombination, Genetic -- Sodium Chloride -- Water-Electrolyte Balance
Аннотация: Effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wildtype strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Yu Krylova, T.; Kargatova, T.V.; Yu Popova, L.; Pechurkin, N.S.

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Population heterogeneity of plasmid-bearing and plasmid-free Bacillus subtilis strains under different environmental conditions / T. Yu. Krylova [et al.] // Microbiology. - 2000. - Vol. 69, Is. 2. - P220-224 . - ISSN 0026-2617
Кл.слова (ненормированные):
Bacillus subtilis -- Heterogeneity -- Introduction -- Microcosm -- Recombinant plasmid
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (KmI)Inf+) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1?M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased. В© 2000 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Krylova, T.Yu.; Popova, L.Yu.; Pechurkin, N.S.; Kashperova, T.A.; Belyavskaya, V.A.

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Population heterogeneity of plasmid-bearing and plasmid-free bacillus subtilis strains under different environmental conditions / T. Yu. Krylova [и др.] // Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 270-275 . - ISSN 0026-3656
Кл.слова (ненормированные):
Bacillus subtilis -- Heterogeneity -- Introduction -- Microcosm -- Recombinant plasmid -- article -- Bacillus subtilis -- culture medium -- genetic recombination -- genetics -- plasmid -- Bacillus subtilis -- Culture Media -- Plasmids -- Recombination, Genetic
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (Km rInf +) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1 ? M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Krylova, T.Yu.; Popova, L.Yu.; Pechurkin, N.S.; Kashperova, T.A.; Belyavskaya, V.A.

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^a621.01.94^2VINITI
Б 86

Бояндин, А. Н.
Плазмидсодержащий и бесплазмидный штаммы Bacillus subtilis при изменении источников углеродного питания [Текст] : научное издание / А. Н. Бояндин, Л. Ю. Попова, Н. С. Печуркин // Биотехнология. - 2000. - N 4. - С. 40-44 . - ISSN 0234-2758

ГРНТИ

62.01.94

РУБ 621.01.94
Рубрики:
BACILLUS SUBTILIS (BACT)
   ПЛАЗМИДСОДЕРЖАЩИЙ ШТАММ
   БЕСПЛАЗМИДНЫЙ ШТАММ
   РОСТОВЫЕ ХАРАКТЕРИСТИКИ
   СРАВНИТЕЛЬНЫЙ АНАЛИЗ
   СМЕНА ИСТОЧНИКОВ УГЛЕРОДА
   ВАКЦИНЫ
   ГЕН 'АЛЬФА'-ИНТЕРФЕРОНА
   ПРОБИОТИКИ
   ОХРАНА ОКРУЖАЮЩЕЙ СРЕДЫ
Аннотация: В данной работе оценивалась способность к росту бесплазмидного и рекомбинантного штаммов Bacillus subtilis в зависимости от изменения источника углеродного питания. Штамм B. subtilis 2335/105, содержащий рекомбинантную плазмиду с клонированными под контролем конститутивного промотора генами 'альфа'-2-интерферона человека, вместе с бесплазмидным штаммом B. subtilis 2335 входит в состав пробиотического препарата "Субалин" и планируется для использования в животноводстве и ветеринарии в качестве лечебно-профилактического (антивирусного и антибактериального) средства, что делает необходимым работы как по повышению эффективности препарата, так и по оценке риска последствий его использования для природных экосистем. Бесплазмидный штамм проявлял значительно лучший рост, чем генетически модифицированный, при выращивании на глюкозной и глицериновой средах, а также после пересева с них. На среде с пептоном не наблюдалось существенных различий между штаммами по данному признаку. Наличие пептона в среде способствует устойчивости микроорганизмов к повышенной конц-ии (5%) хлорида натрия. Рассматриваются условия доминирования бесплазмидного или плазмидсодержащего штаммов. Россия, Ин-т биофизики СО РАН, Красноярск, Академгородок, 660036. Библ. 6
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Попова, Л.Ю.; Печуркин, Н.С.

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^a341.27.21.09.02^2VINITI
И 39

Изучение гетерогенности в популяциях плазмидсодержащего и бесплазмидного штаммов Bacillus subtilis в разных условиях существования [Текст] : научное издание / Т. Ю. Крылова [и др.] // Микробиология. - 2000. - Т. 69, N 2. - С. 270-275 . - ISSN 0026-3656

ГРНТИ

34.27.21

РУБ 341.27.21.09.02
Рубрики:
ПОПУЛЯЦИИ
   РЕКОМБИНАНТНЫЕ
   БЕСПЛАЗМИДНЫЕ
   ГЕТЕРОГЕННОСТЬ
   ВОДНЫЕ МИКРОКОСМЫ
   РАЗНЫЕ УСЛОВИЯ СУЩЕСТВОВАНИЯ
   BACILLUS SUBTILIS (BACT.)
   ШТАММ L335
Аннотация: Проведено сравнение гетерогенности популяций рекомбинантного и бесплазмидного штаммов Bacillus subtilis при интродукции в водные микрокосмы. Бесплазмидный штамм B. subtilis 2335 сохранялся как в форме вегетативных клеток, так и в форме спор, рекомбинантный штамм B. subtilis 2335/105 (Km{r}Inf{+}) - преимущественно в форме спор. У "споровых" изолятов рекомбинантного штамма отмечали сохранение исходной копийности плазмиды, а у "вегетативных" изолятов - снижение исходной копийности. У изолятов B. subtilis 2335/105, полученных из микрокосмов, и вариантов, полученных после 10 последовательных пассажей (среды М9 и 0.1*М9 с Km и без Km), отмечали изменения по копийности, устойчивости к Km и максимальному приросту биомассы в зависимости от условий выделения. На среде М9 (независимо от присутствия Km) у 50 и более процентов вариантов наблюдали снижение копийности. На 0.1*М9 - около 70% на среде с Km и 90% вариантов на среде без Km сохраняли исходную копийность, что в основном коррелировало с устойчивостью к Km. У всех вариантов, выделенных со сред с Km, наблюдали сохранение исходного максимального прироста биомассы. У 70 и более процентов вариантов, выделенных со сред без Km, отмечали увеличение прироста биомассы. Россия, Ин-т биофизики СО РАН, КрасноярскU
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Крылова, Т.Ю.; Попова, Л.Ю.; Печуркин, Н.С.; Кашперова, Т.А.; Белявская, В.А.

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10.

Effect of salinity on the adaptive capacity of recombinant strains of Escherichia coli and Bacillus subtilis / A. N. Boyandin [et al.] // Microbiology. - 2000. - Vol. 69, Is. 2. - P196-199 . - ISSN 0026-2617
Кл.слова (ненормированные):
Genetically modified microorganisms -- Salinity -- Salt resistance
Аннотация: The effect of different concentrations of salts on natural and recombinant strains of Bacillus subtilis and Escherichia coli was studied. The recombinant strain of B. subtilis was found to be more osmotolerant than the wild-type strain of this bacterium, whereas the opposite situation was observed for the recombinant and wild-type strains of E. coli. Some salts exerted a bacteriostatic effect on E. coli and B. subtilis. The adaptive capacity of recombinant strains depended on the number of plasmid copies in the cells. The introduction of recombinant bacteria into model ecosystems resulted in the generation of their variants with increased osmotolerance. В© 2000 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Lobova, T.I.; Krylova, T.Yu.; Kargatova, T.V.; Popova, L.Yu.; Pechurkin, N.S.

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11.

Population dynamics of transgenic microorganisms in the different microecosystem conditions [Text] / L. Y. Popova [et al.] ; ed. , AN Boyand // SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH : ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P. 1571-1579, DOI 10.1016/S0273-1177(01)00248-4. - Cited References: 8 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
GENETICALLY-ENGINEERED MICROORGANISMS
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap(r)Lux(+)) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human alpha 2-interferon Km(r)lnf(+)), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Y.; Lobova, T.I.; Krylova, T.Y.; Kargatova, T.V.; Maksimova, E.E.; Boyandin, A.N.; Pechurkin, N.S.; Boyand, , AN \ed.\

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12.

Stability of recombinant plasmids in transgenic microorganisms under different environmental conditions / L. Yu. Popova [и др.] // Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 796-803 . - ISSN 0026-3656
Кл.слова (ненормированные):
Antibiotic resistance -- Bioluminescence interferon -- Recombinant plasmids -- Transgenic microorganisms -- article -- Bacillus subtilis -- culture medium -- Escherichia coli -- genetic engineering -- genetic recombination -- genetics -- R factor -- Bacillus subtilis -- Culture Media -- Escherichia coli -- Genetic Engineering -- R Factors -- Recombination, Genetic
Аннотация: The copy number of R plasmids weakly depends on the selective pressure of the respective antibiotic but does depend on the physiology of the host species and the type of plasmids and cloned genes, whose expression leads to a further load on the biosynthetic apparatus of cells. The last factor is critical in the maintenance of recombinant plasmids in transgenic microorganisms.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Maksimova, E.E.; Lobova, T.I.; Kargatova, T.V.; Boyandin, A.N.; Krylova, T.Yu.; Pechurkin, N.S.

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13.

Population dynamics of transgenic microorganisms in the different microecosystem conditions / L. Yu. Popova [et al.] // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1571-1579, DOI 10.1016/S0273-1177(01)00248-4 . - ISSN 0273-1177
Кл.слова (ненормированные):
Ecosystems -- Escherichia coli -- Genes -- Interferons -- Luminescence -- Vaccines -- Microcosms -- Microorganisms -- fresh water -- microorganism -- adaptation -- article -- Bacillus subtilis -- comparative study -- ecosystem -- Escherichia coli -- gene expression -- genetics -- growth, development and aging -- hazardous waste -- microbiology -- Micrococcus -- osmotic pressure -- plasmid -- population dynamics -- risk assessment -- transgenic organism -- Adaptation, Physiological -- Bacillus subtilis -- Containment of Biohazards -- Ecosystem -- Escherichia coli -- Fresh Water -- Gene Expression -- Micrococcus -- Organisms, Genetically Modified -- Osmotic Pressure -- Plasmids -- Population Dynamics -- Risk Assessment -- Water Microbiology
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap1Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human ? 2-interferon Km1Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Popova, L.Yu.; Lobova, T.I.; Krylova, T.Yu.; Kargatova, T.V.; Maksimova, E.E.; Boyandin, A.N.; Pechurkin, N.S.

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14.

On the problem of genome redundancy in viruses and prokaryotes [Text] / M. G. Sadovsky // Russ. J. Genet. - 2002. - Vol. 38, Is. 5. - P. 575-581, DOI 10.1023/A:1015503617023. - Cited References: 10 . - ISSN 1022-7954

РУБ Genetics & Heredity

Аннотация: A specific index of nucleotide sequence redundancy, the specific restriction length of a finite frequency dictionary, was determined for a complete set of genes in some viral genomes and a genome of a bacterium, Bacillus subtilis. The distribution of the gene number over the specific restriction length was shown to be bimodal for viral genomes and unimodal for the Bac. subtilis genome. These results agree with earlier data.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sadovsky, M.G.

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15.

The architectonics of colonies of Bacillus subtilis 2335 [Text] / A. P. Puzyr' [et al.] // Microbiology. - 2002. - Vol. 71, Is. 1. - P. 57-64, DOI 10.1023/A:1017950316748. - Cited References: 12 . - ISSN 0026-2617

РУБ Microbiology

Кл.слова (ненормированные):
electron microscopy -- Bacillus subtills -- spores -- vegetative cells -- glycocalyx -- architectonics of bacterial colonies
Аннотация: Colonies grown from vegetative Bacillus subtilis 2335 cells had a standard structure, with bacillar cells occupying the whole colony volume. At the same time, the colonies of this bacterium grown from germinated spores had an abnormal structure characterized by the location of cells in a surface layer 100-200 mum thick at the colony boundary with the air. The glycocalyx of the colonies grown from spores was characterized by a wetting angle theta(e) of 120degrees-160degrees, whereas that of the colonies grown from vegetative cells had an angle theta(c) as low as 5degrees-30degrees. It is suggested that spores and vegetative cells follow different strategies of substrate colonization and that the architectonics of bacterial colonies is determined by the physicochemical properties of the glycocalyx.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Puzyr', A.P.; Mogil'naya, O.A.; Krylova, T.Y.; Popova, L.Y.

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16.

Development and morphological features of biofilms formed by transgenic and wild type strains of Bacillus subtilis [Text] / O. A. Mogilnaya, T. Y. Krylova, L. Y. Popova // Microbiol. Res. - 2003. - Vol. 158, Is. 4. - P. 327-335, DOI 10.1078/0944-5013-00212. - Cited References: 17 . - ISSN 0944-5013

РУБ Microbiology
Рубрики:
ANTIBIOTIC-RESISTANCE
MICROBIAL BIOFILMS
COMMUNITY
Кл.слова (ненормированные):
Bacillus subtilis -- biofilms -- transgenic microorganisms -- electron microscopy
Аннотация: The study addressed the ability of the transgenic strain (TM) B. subtilis 2335/pBMB 105 (Km(r)Inf(+)) to form biofilms on the surface of liquid media of various compositions, inoculated with vegetative cells and spores. The morphological features of these biofilms do not differ from those of the films formed by the recipient strain (WT) B. subtilis 2335 (Km(s)). However, the TM and the natural one differ in the dynamics of biofilm formation and the cellular composition of the films. Biofilms of the TM are formed earlier, develop at a higher rate, but decompose later than the films of the WT. When the medium is inoculated with vegetative cells, sporulation in the biofilms of both strains undergoes glucose repression; no such effect is observed when the medium is inoculated with spores. The TM does not form films when the medium is inoculated with spores and supplemented with glycerin and kanamycin.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Mogilnaya, O.A.; Krylova, T.Y.; Popova, L.Y.

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17.

Psychrophilic and psychrotolerant heterotrophic microorganisms of Middle Siberian karst cavities [Text] / S. V. Khizhnyak [et al.] // Russ. J. Ecol. - 2003. - Vol. 34, Is. 4. - P. 231-235, DOI 10.1023/A:1024537513439. - Cited References: 12 . - ISSN 1067-4136

РУБ Ecology

Кл.слова (ненормированные):
psychrophilic and psychrotolerant microorganisms -- karst cavities -- caves -- heterotrophic microorganisms
Аннотация: The natural microflora of Middle Siberian karst cavities, which comprises psychrotolerant bacteria and fungi capable of growing at 3-15 and 3...+28degreesC, respectively, has been studied. Bacteria are ubiquitous in caves, their count varying from 10(3) to 10(7) cells/g ground. The bacteria have been identified as Pseudomonas, Arthrobacter, Bacillus, and coryneform bacteria. Fungi have been found in places exposed to increased anthropogenic impact, their count being as large as 10(6) to 10(7) cells/g ground. Mucor Penicillium, and Chrysosporium were dominant fungal genera.

WOS
Держатели документа:
Krasnoyarsk State Agrarian Univ, Krasnoyarsk 660001, Russia
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Khizhnyak, S.V.; Tausheva, I.V.; Berezikova, A.A.; Nesterenko, E.V.; Rogozin, D.Y.

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18.

The morphological characteristics and the dynamics of biofilms formed by a transgenic Bacillus subtilis strain [Text] / O. A. Mogil'naya, T. Y. Krylova, L. Y. Popova // Microbiology. - 2003. - Vol. 72, Is. 4. - P. 509-510, DOI 10.1023/A:1025065311507. - Cited References: 5 . - ISSN 0026-2617

РУБ Microbiology

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
Mogil'naya, O.A.; Krylova, T.Y.; Popova, L.Y.

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19.

^a621.99.29^2VINITI
П 86

Психрофильные и психротолерантные гетеротрофные микроорганизмы карстовых полостей Средней Сибири [Текст] : научное издание / С. В. Хижняк [и др.] // Экология. - 2003. - N 4. - С. 261-266 . - ISSN 0367-0597

ГРНТИ

62.99.29

РУБ 621.99.29
Рубрики:
ПСИХРОФИЛЬНЫЕ МИКРООРГАНИЗМЫ
   ПСИХРОТОЛЕРАНТНЫЕ МИКРООРГАНИЗМЫ
   ЧИСЛЕННОСТЬ
   ИДЕНТИФИКАЦИЯ
   ПОДЗЕМНЫЕ ЭКОСИСТЕМЫ
   ПЕЩЕРЫ
Аннотация: Исследовали естественную микрофлору карстовых полостей Средней Сибири, представленную психрофильными и психротолерантными бактериями и грибами, способными к росту при т-ре +3...+15'ГРАДУС'C и +3...+28'ГРАДУС'C соответственно. Бактерии в пещерах распространены повсеместно, их численность колеблется от 10{3} до 10{7} клеток/г грунта. Идентификация показала наличие родов Pseudomonas, Arthrobacter, Bacillus и коринебактерий. Грибы встречаются в местах с повышенной антропогенной нагрузкой, их численность достигает 10{6}-10{7} колониеобразующих единиц/г грунта. Среди грибов доминируют представители родов Mucor, Penicillium, Chrysosporium. Россия, Красноярский гос. аграрный ун-т, Красноярск. Библ. 12
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Хижняк, С.В.; Таушева, И.В.; Березикова, А.А.; Нестеренко, Е.В.; Рогозин, Д.Ю.

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20.

Psychrophilic and psychrotolerant heterotrophic microorganisms of Middle Siberian karst cavities / S. V. Khizhnyak [et al.] // Russian Journal of Ecology. - 2003. - Vol. 34, Is. 4. - P231-235, DOI 10.1023/A:1024537513439 . - ISSN 1067-4136
Кл.слова (ненормированные):
Caves -- Heterotrophic microorganisms -- Karst cavities -- Psychorophilic and psychrotolerant microorganisms -- bacterium -- cavernicolous species -- fungus -- heterotrophy -- karst -- low temperature -- Russian Federation -- Arthrobacter -- Bacteria (microorganisms) -- Chrysosporium -- Corynebacteriaceae -- Fungi -- Mucor -- Penicillium -- Pseudomonas
Аннотация: The natural microflora of Middle Siberian karst cavities, which comprises psychrotolerant bacteria and fungi capable of growing at 3-15 and 3...+28В°C, respectively, has been studied. Bacteria are ubiquitous in caves, their count varying from 103 to 107 cells/g ground. The bacteria have been identified as Pseudomonas, Arthrobacter, Bacillus, and coryneform bacteria. Fungi have been found in places exposed to increased anthropogenic impact, their count being as large as 106 to 107 cells/g ground. Mucor, Penicillium, and Chrysosporium were dominant fungal genera.

Scopus
Держатели документа:
Krasnoyarsk State Agrarian Univ., Pr. Mira 88, Krasnoyarsk 660001, Russian Federation
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Khizhnyak, S.V.; Tausheva, I.V.; Berezikova, A.A.; Nesterenko, E.V.; Rogozin, D.Yu.

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