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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Хижняк С.В., Таушева И.В., Березикова А.А., Нестеренко Е.В., Рогозин Д.Ю.
Заглавие : Психрофильные и психротолерантные гетеротрофные микроорганизмы карстовых полостей Средней Сибири : научное издание
Место публикации : Экология. - 2003. - N 4. - С. 261-266. - ISSN 0367-0597
ГРНТИ : 62.99.29
Предметные рубрики: ПСИХРОФИЛЬНЫЕ МИКРООРГАНИЗМЫ
ПСИХРОТОЛЕРАНТНЫЕ МИКРООРГАНИЗМЫ
ЧИСЛЕННОСТЬ
ИДЕНТИФИКАЦИЯ
ПОДЗЕМНЫЕ ЭКОСИСТЕМЫ
ПЕЩЕРЫ
Аннотация: Исследовали естественную микрофлору карстовых полостей Средней Сибири, представленную психрофильными и психротолерантными бактериями и грибами, способными к росту при т-ре +3...+15'ГРАДУС'C и +3...+28'ГРАДУС'C соответственно. Бактерии в пещерах распространены повсеместно, их численность колеблется от 10{3} до 10{7} клеток/г грунта. Идентификация показала наличие родов Pseudomonas, Arthrobacter, Bacillus и коринебактерий. Грибы встречаются в местах с повышенной антропогенной нагрузкой, их численность достигает 10{6}-10{7} колониеобразующих единиц/г грунта. Среди грибов доминируют представители родов Mucor, Penicillium, Chrysosporium. Россия, Красноярский гос. аграрный ун-т, Красноярск. Библ. 12
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Крылова Т.Ю., Попова Л.Ю., Печуркин Н.С., Кашперова Т.А., Белявская В.А.
Заглавие : Изучение гетерогенности в популяциях плазмидсодержащего и бесплазмидного штаммов Bacillus subtilis в разных условиях существования : научное издание
Место публикации : Микробиология. - 2000. - Т. 69, N 2. - С. 270-275. - ISSN 0026-3656
ГРНТИ : 34.27.21
Предметные рубрики: ПОПУЛЯЦИИ
РЕКОМБИНАНТНЫЕ
БЕСПЛАЗМИДНЫЕ
ГЕТЕРОГЕННОСТЬ
ВОДНЫЕ МИКРОКОСМЫ
РАЗНЫЕ УСЛОВИЯ СУЩЕСТВОВАНИЯ
BACILLUS SUBTILIS (BACT.)
ШТАММ L335
Аннотация: Проведено сравнение гетерогенности популяций рекомбинантного и бесплазмидного штаммов Bacillus subtilis при интродукции в водные микрокосмы. Бесплазмидный штамм B. subtilis 2335 сохранялся как в форме вегетативных клеток, так и в форме спор, рекомбинантный штамм B. subtilis 2335/105 (Km{r}Inf{+}) - преимущественно в форме спор. У "споровых" изолятов рекомбинантного штамма отмечали сохранение исходной копийности плазмиды, а у "вегетативных" изолятов - снижение исходной копийности. У изолятов B. subtilis 2335/105, полученных из микрокосмов, и вариантов, полученных после 10 последовательных пассажей (среды М9 и 0.1*М9 с Km и без Km), отмечали изменения по копийности, устойчивости к Km и максимальному приросту биомассы в зависимости от условий выделения. На среде М9 (независимо от присутствия Km) у 50 и более процентов вариантов наблюдали снижение копийности. На 0.1*М9 - около 70% на среде с Km и 90% вариантов на среде без Km сохраняли исходную копийность, что в основном коррелировало с устойчивостью к Km. У всех вариантов, выделенных со сред с Km, наблюдали сохранение исходного максимального прироста биомассы. У 70 и более процентов вариантов, выделенных со сред без Km, отмечали увеличение прироста биомассы. Россия, Ин-т биофизики СО РАН, КрасноярскU
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Бояндин А.Н., Попова Л.Ю., Печуркин Н.С.
Заглавие : Плазмидсодержащий и бесплазмидный штаммы Bacillus subtilis при изменении источников углеродного питания : научное издание
Место публикации : Биотехнология. - 2000. - N 4. - С. 40-44. - ISSN 0234-2758
ГРНТИ : 62.01.94
Предметные рубрики: BACILLUS SUBTILIS (BACT)
ПЛАЗМИДСОДЕРЖАЩИЙ ШТАММ
БЕСПЛАЗМИДНЫЙ ШТАММ
РОСТОВЫЕ ХАРАКТЕРИСТИКИ
СРАВНИТЕЛЬНЫЙ АНАЛИЗ
СМЕНА ИСТОЧНИКОВ УГЛЕРОДА
ВАКЦИНЫ
ГЕН 'АЛЬФА'-ИНТЕРФЕРОНА
ПРОБИОТИКИ
ОХРАНА ОКРУЖАЮЩЕЙ СРЕДЫ
Аннотация: В данной работе оценивалась способность к росту бесплазмидного и рекомбинантного штаммов Bacillus subtilis в зависимости от изменения источника углеродного питания. Штамм B. subtilis 2335/105, содержащий рекомбинантную плазмиду с клонированными под контролем конститутивного промотора генами 'альфа'-2-интерферона человека, вместе с бесплазмидным штаммом B. subtilis 2335 входит в состав пробиотического препарата "Субалин" и планируется для использования в животноводстве и ветеринарии в качестве лечебно-профилактического (антивирусного и антибактериального) средства, что делает необходимым работы как по повышению эффективности препарата, так и по оценке риска последствий его использования для природных экосистем. Бесплазмидный штамм проявлял значительно лучший рост, чем генетически модифицированный, при выращивании на глюкозной и глицериновой средах, а также после пересева с них. На среде с пептоном не наблюдалось существенных различий между штаммами по данному признаку. Наличие пептона в среде способствует устойчивости микроорганизмов к повышенной конц-ии (5%) хлорида натрия. Рассматриваются условия доминирования бесплазмидного или плазмидсодержащего штаммов. Россия, Ин-т биофизики СО РАН, Красноярск, Академгородок, 660036. Библ. 6
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Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Gros J.-B., Lasseur C., Tikhomirov A.A., Manukovsky N.S., Ushakova S.A., Zolotukhin I.G., Tirranen L.S., Borodina E.V., Kovalev V.S.
Заглавие : Wheat growth on neutral and soil-like substrates: Carbon dioxide exchange and microflora
Место публикации : Acta Horticulturae. - 2004. - Vol. 644. - С. 243-248
Ключевые слова (''Своб.индексиров.''): carbon dioxide--closed ecological system
Аннотация: The soil-like substrate (SLS), which is a potential candidate for employment in closed ecological systems, has been tested. CO2 exchange, composition and numbers of soil microflora have been examined in .wheat-SLS. system. The results produced have been compared to analogous characteristics of .wheat-neutral substrate. system. A hydroponic method was used under wheat growing on the neutral substrate (expanded clay aggregate). Plants for both studies were grown in closed environment from seed to physiological maturity. In the .wheat-SLS. system, the net photosynthetic rate of canopy was positive in the course of 6.55 days after planting. The net photosynthetic rate of canopy in .wheat-neutral substrate. system was positive in the entire course of vegetation. According to calculations in the course of vegetation, photosynthesis has withdrawn 3.28 kg m-2 CO2 from the .wheat-SLS. system and 3.40 kg m-2 CO2 from the .wheat-neutral substrate. system. On the SLS dominant among bacteria were the spore-forming bacteria from Bacillus genus, among fungi . from Trichoderma genus. In the hydroponic cultivation on neutral substrate dominant were bacteria from Pseudomonas genus, most commonly found fungi were species from Fusarium and Botrytis genera.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogil'naya O.A., Krylova T.Y., Popova L.Y.
Заглавие : The morphological characteristics and the dynamics of biofilms formed by a transgenic Bacillus subtilis strain
Колич.характеристики :2 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2003. - Vol. 72, Is. 4. - P509-510. - ISSN 0026-2617, DOI 10.1023/A:1025065311507
Примечания : Cited References: 5
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S., Streltsova N., Volova T.
Заглавие : The effect of the pesticide delivery method on the microbial community of field soil
Место публикации : Environ. Sci. Pollut. Res.: Springer Science and Business Media Deutschland GmbH, 2020. - Article in press. - ISSN 09441344 (ISSN), DOI 10.1007/s11356-020-11228-7
Аннотация: The study deals with the effects of herbicides (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl) and fungicides (tebuconazole, epoxiconazole, azoxystrobin) applied to soil as free pesticides or as slow release formulations embedded in a biodegradable composite matrix on the structure of the soil microbial community. The matrix consisted of a natural biopolymer poly-3-hydroxybutyrate [P(3HB)] and a filler—one of the natural materials (peat, clay, and wood flour). The soil microbial community was characterized, including the major eco-trophic groups of bacteria, dominant taxa of bacteria and fungi, and primary P(3HB)-degrading microorganisms, such as Pseudomonas, Bacillus, Pseudarthrobacter, Streptomyces, Penicillium, and Talaromyces. The addition of free pesticides adversely affected the abundance of soil microorganisms; the decrease varied from 1.4 to 56.0 times for different types of pesticides. The slow release pesticide formulations, in contrast to the free pesticides, exerted a much weaker effect on soil microorganisms, no significant inhibition in the abundance of saprotrophic bacteria was observed, partly due to the positive effects of the composite matrix (polymer/natural material), which was a supplementary substrate for microorganisms. The slow release fungicide formulations, like the free fungicides, reduced the total abundance of fungi and inhibited the development of the phytopathogens Fusarium and Alternaria. Thus, slow release formulations of pesticides preserve the bioremediation potential of soil microorganisms, which are the main factor of removing xenobiotics from the biosphere. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova, Svetlana, Streltsova, Nadezhda, Volova, Tatiana
Заглавие : The effect of the pesticide delivery method on the microbial community of field soil
Колич.характеристики :17 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation, project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]
Место публикации : Environ. Sci. Pollut. Res.: SPRINGER HEIDELBERG, 2020. - Article in press. - ISSN 0944-1344, DOI 10.1007/s11356-020-11228-7. - ISSN 1614-7499(eISSN)
Примечания : Cited References:119. - This study was financially supported by the Ministry of Science and Higher Education of the Russian Federation, project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328).
Предметные рубрики: CONTROLLED-RELEASE
2,4-DICHLOROPHENOXYACETIC ACID
DEGRADATION
Аннотация: The study deals with the effects of herbicides (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl) and fungicides (tebuconazole, epoxiconazole, azoxystrobin) applied to soil as free pesticides or as slow release formulations embedded in a biodegradable composite matrix on the structure of the soil microbial community. The matrix consisted of a natural biopolymer poly-3-hydroxybutyrate [P(3HB)] and a filler-one of the natural materials (peat, clay, and wood flour). The soil microbial community was characterized, including the major eco-trophic groups of bacteria, dominant taxa of bacteria and fungi, and primary P(3HB)-degrading microorganisms, such asPseudomonas,Bacillus,Pseudarthrobacter,Streptomyces,Penicillium, andTalaromyces. The addition of free pesticides adversely affected the abundance of soil microorganisms; the decrease varied from 1.4 to 56.0 times for different types of pesticides. The slow release pesticide formulations, in contrast to the free pesticides, exerted a much weaker effect on soil microorganisms, no significant inhibition in the abundance of saprotrophic bacteria was observed, partly due to the positive effects of the composite matrix (polymer/natural material), which was a supplementary substrate for microorganisms. The slow release fungicide formulations, like the free fungicides, reduced the total abundance of fungi and inhibited the development of the phytopathogensFusariumandAlternaria. Thus, slow release formulations of pesticides preserve the bioremediation potential of soil microorganisms, which are the main factor of removing xenobiotics from the biosphere.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr' A.P., Mogil'naya O.A., Krylova T.Y., Popova L.Y.
Заглавие : The architectonics of colonies of Bacillus subtilis 2335
Колич.характеристики :8 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2002. - Vol. 71, Is. 1. - P57-64. - ISSN 0026-2617, DOI 10.1023/A:1017950316748
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): electron microscopy--bacillus subtills--spores--vegetative cells--glycocalyx--architectonics of bacterial colonies
Аннотация: Colonies grown from vegetative Bacillus subtilis 2335 cells had a standard structure, with bacillar cells occupying the whole colony volume. At the same time, the colonies of this bacterium grown from germinated spores had an abnormal structure characterized by the location of cells in a surface layer 100-200 mum thick at the colony boundary with the air. The glycocalyx of the colonies grown from spores was characterized by a wetting angle theta(e) of 120degrees-160degrees, whereas that of the colonies grown from vegetative cells had an angle theta(c) as low as 5degrees-30degrees. It is suggested that spores and vegetative cells follow different strategies of substrate colonization and that the architectonics of bacterial colonies is determined by the physicochemical properties of the glycocalyx.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gros J.B., Lasseur Ch., Tikhomirov A.A., Manukovsky N.S., Kovalev V.S., Ushakova S.A., Zolotukhin I.G., Tirranen L.S., Karnachuk R.A., Dorofeev V.Yu.
Заглавие : Testing soil-like substrate for growing plants in bioregenerative life support systems
Место публикации : Advances in Space Research. - 2005. - Vol. 36, Is. 7. - С. 1312-1318. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2005.05.079
Ключевые слова (''Своб.индексиров.''): life support system--matter recycling--plants--soil-like substrate--biodiversity--correlation methods--growth kinetics--hormones--plants (botany)--recycling--soils--bioregeneration--life support system--matter recycling--soil-like substrate--space research
Аннотация: We studied soil-like substrate (SLS) as a potential candidate for plant cultivation in bioregenerative life support systems (BLSS). The SLS was obtained by successive conversion of wheat straw by oyster mushrooms and worms. Mature SLS contained 9.5% humic acids and 4.9% fulvic acids. First, it was shown that wheat, bean and cucumber yields as well as radish yields when cultivated on mature SLS were comparable to yields obtained on a neutral substrate (expanded clay aggregate) under hydroponics. Second, the possibility of increasing wheat and radish yields on the SLS was assessed at three levels of light intensity: 690, 920 and 1150 ?mol m-2 s-1 of photosynthetically active radiation (PAR). The highest wheat yield was obtained at 920 ?mol m-2 s-1, while radish yield increased steadily with increasing light intensity. Third, long-term SLS fertility was tested in a BLSS model with mineral and organic matter recycling. Eight cycles of wheat and 13 cycles of radish cultivation were carried out on the SLS in the experimental system. Correlation coefficients between SLS nitrogen content and total wheat biomass and grain yield were 0.92 and 0.97, respectively, and correlation coefficients between nitrogen content and total radish biomass and edible root yield were 0.88 and 0.87, respectively. Changes in hormone content (auxins, gibberellins, cytokinins and abscisic acid) in the SLS during matter recycling did not reduce plant productivity. Quantitative and species compositions of the SLS and irrigation water microflora were also investigated. Microbial community analysis of the SLS showed bacteria from Bacillus, Pseudomonas, Proteus, Nocardia, Mycobacterium, Arthrobacter and Enterobacter genera, and fungi from Trichoderma, Penicillium, Fusarium, Aspergillus, Mucor, Botrytis, and Cladosporium genera. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
Scopus
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Yu., Maksimova E.E., Lobova T.I., Kargatova T.V., Boyandin A.N., Krylova T.Yu., Pechurkin N.S.
Заглавие : Stability of recombinant plasmids in transgenic microorganisms under different environmental conditions
Место публикации : Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 796-803. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): antibiotic resistance--bioluminescence interferon--recombinant plasmids--transgenic microorganisms--article--bacillus subtilis--culture medium--escherichia coli--genetic engineering--genetic recombination--genetics--r factor--bacillus subtilis--culture media--escherichia coli--genetic engineering--r factors--recombination, genetic
Аннотация: The copy number of R plasmids weakly depends on the selective pressure of the respective antibiotic but does depend on the physiology of the host species and the type of plasmids and cloned genes, whose expression leads to a further load on the biosynthetic apparatus of cells. The last factor is critical in the maintenance of recombinant plasmids in transgenic microorganisms.
Scopus
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Khizhnyak S.V., Tausheva I.V., Berezikova A.A., Nesterenko E.V., Rogozin D.Yu.
Заглавие : Psychrophilic and psychrotolerant heterotrophic microorganisms of Middle Siberian karst cavities
Место публикации : Russian Journal of Ecology. - 2003. - Vol. 34, Is. 4. - С. 231-235. - ISSN 10674136 (ISSN) , DOI 10.1023/A:1024537513439
Ключевые слова (''Своб.индексиров.''): caves--heterotrophic microorganisms--karst cavities--psychorophilic and psychrotolerant microorganisms--bacterium--cavernicolous species--fungus--heterotrophy--karst--low temperature--russian federation--arthrobacter--bacteria (microorganisms)--chrysosporium--corynebacteriaceae--fungi--mucor--penicillium--pseudomonas
Аннотация: The natural microflora of Middle Siberian karst cavities, which comprises psychrotolerant bacteria and fungi capable of growing at 3-15 and 3...+28В°C, respectively, has been studied. Bacteria are ubiquitous in caves, their count varying from 103 to 107 cells/g ground. The bacteria have been identified as Pseudomonas, Arthrobacter, Bacillus, and coryneform bacteria. Fungi have been found in places exposed to increased anthropogenic impact, their count being as large as 106 to 107 cells/g ground. Mucor, Penicillium, and Chrysosporium were dominant fungal genera.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Khizhnyak S.V., Tausheva I.V., Berezikova A.A., Nesterenko E.V., Rogozin D.Y.
Заглавие : Psychrophilic and psychrotolerant heterotrophic microorganisms of Middle Siberian karst cavities
Колич.характеристики :5 с
Место публикации : Russ. J. Ecol.: MAIK NAUKA/INTERPERIODICA, 2003. - Vol. 34, Is. 4. - P231-235. - ISSN 1067-4136, DOI 10.1023/A:1024537513439
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): psychrophilic and psychrotolerant microorganisms--karst cavities--caves--heterotrophic microorganisms
Аннотация: The natural microflora of Middle Siberian karst cavities, which comprises psychrotolerant bacteria and fungi capable of growing at 3-15 and 3...+28degreesC, respectively, has been studied. Bacteria are ubiquitous in caves, their count varying from 10(3) to 10(7) cells/g ground. The bacteria have been identified as Pseudomonas, Arthrobacter, Bacillus, and coryneform bacteria. Fungi have been found in places exposed to increased anthropogenic impact, their count being as large as 10(6) to 10(7) cells/g ground. Mucor Penicillium, and Chrysosporium were dominant fungal genera.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krylova T.Yu., Popova L.Yu., Pechurkin N.S., Kashperova T.A., Belyavskaya V.A.
Заглавие : Population heterogeneity of plasmid-bearing and plasmid-free bacillus subtilis strains under different environmental conditions
Место публикации : Mikrobiologiya. - 2000. - Vol. 69, Is. 2. - С. 270-275. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacillus subtilis--heterogeneity--introduction--microcosm--recombinant plasmid--article--bacillus subtilis--culture medium--genetic recombination--genetics--plasmid--bacillus subtilis--culture media--plasmids--recombination, genetic
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (Km rInf +) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1 ? M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krylova T.Yu., Popova L.Yu., Pechurkin N.S., Kashperova T.A., Belyavskaya V.A.
Заглавие : Population heterogeneity of plasmid-bearing and plasmid-free Bacillus subtilis strains under different environmental conditions
Место публикации : Microbiology. - 2000. - Vol. 69, Is. 2. - С. 220-224. - ISSN 00262617 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacillus subtilis--heterogeneity--introduction--microcosm--recombinant plasmid
Аннотация: The population heterogeneity of recombinant and plasmid-free Bacillus subtilis strains introduced into aquatic microcosms was studied. After introduction, the population of the plasmid-free strain B. subtilis 2335 in microcosms has long been represented by both vegetative cells and spores, whereas, already ten days after introduction, the population of the recombinant strain B. subtilis 2335/105 (KmI)Inf+) was represented only by spores. The number of plasmid copies in the spore isolates of the recombinant strain was the same as before introduction, but the plasmid abundance in the vegetative isolates of this strain decreased. The isolates of B. subtilis 2335/105 obtained from microcosms and the variants of this strain obtained by ten successive subcultures on M9 and 0.1?M9 media with and without kanamycin (Km) differed in the number of plasmid copies, Km resistance, and maximum biomass yield during batch cultivation. Irrespective of the presence of Km, more than 50% of the variants subcultured on M9 medium showed reduced plasmid abundance. At the same time, about 70% of the variants subcultured on 0.1 ? M9 medium with Km and 90% of the variants subcultured on the same medium without Km retained the initial number of plasmid copies. The variants subcultured on media with Km retained the initial biomass level. In more than 70% of the variants isolated from media without Km, the biomass yield increased. В© 2000 MAIK "Nauka/Interperiodica".
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Yu., Lobova T.I., Krylova T.Yu., Kargatova T.V., Maksimova E.E., Boyandin A.N., Pechurkin N.S.
Заглавие : Population dynamics of transgenic microorganisms in the different microecosystem conditions
Место публикации : Advances in Space Research. - 2001. - Vol. 27, Is. 9. - С. 1571-1579. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(01)00248-4
Ключевые слова (''Своб.индексиров.''): ecosystems--escherichia coli--genes--interferons--luminescence--vaccines--microcosms--microorganisms--fresh water--microorganism--adaptation--article--bacillus subtilis--comparative study--ecosystem--escherichia coli--gene expression--genetics--growth, development and aging--hazardous waste--microbiology--micrococcus--osmotic pressure--plasmid--population dynamics--risk assessment--transgenic organism--adaptation, physiological--bacillus subtilis--containment of biohazards--ecosystem--escherichia coli--fresh water--gene expression--micrococcus--organisms, genetically modified--osmotic pressure--plasmids--population dynamics--risk assessment--water microbiology
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap1Lux+) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human ? 2-interferon Km1Inf+), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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16.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Popova L.Y., Lobova T.I., Krylova T.Y., Kargatova T.V., Maksimova E.E., Boyandin A.N., Pechurkin N.S.
Заглавие : Population dynamics of transgenic microorganisms in the different microecosystem conditions
Колич.характеристики :9 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH: ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P1571-1579. - ISBN 0273-1177, DOI 10.1016/S0273-1177(01)00248-4
Примечания : Cited References: 8
Предметные рубрики: GENETICALLY-ENGINEERED MICROORGANISMS
Аннотация: The role of key environmental factors in adaptation of spore-forming and non-spore-forming transgenic microorganisms (TM) have been studied in model ecosystems. Model TM Escherichia coli Z905 (bearing plasmid genes of bacterial luminescence Ap(r)Lux(+)) has been found to have a higher adaptation potential than TM Bacillus subtilis 2335/105 (bearing genes of human alpha 2-interferon Km(r)lnf(+)), planned for employment as a living vaccine under varying environmental conditions. Effects of abiotic factors on migration of natural and recombinant plasmids between microorganisms under model ecosystem conditions has been estimated. The transgenic microorganisms with low copy number survived better under introduction conditions in the microcosms studied. This trend has been shown to be independent of the microcosm type and its complexity. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sadovsky M.G.
Заглавие : On the problem of genome redundancy in viruses and prokaryotes
Колич.характеристики :7 с
Место публикации : Russ. J. Genet.: KLUWER ACADEMIC/PLENUM PUBL, 2002. - Vol. 38, Is. 5. - P575-581. - ISSN 1022-7954, DOI 10.1023/A:1015503617023
Примечания : Cited References: 10
Аннотация: A specific index of nucleotide sequence redundancy, the specific restriction length of a finite frequency dictionary, was determined for a complete set of genes in some viral genomes and a genome of a bacterium, Bacillus subtilis. The distribution of the gene number over the specific restriction length was shown to be bimodal for viral genomes and unimodal for the Bac. subtilis genome. These results agree with earlier data.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana G., Prudnikova, Svetlana V., Vinogradova, Olga N., Syrvacheva, Darya A., Shishatskaya, Ekaterina I.
Заглавие : Microbial Degradation of Polyhydroxyalkanoates with Different Chemical Compositions and Their Biodegradability
Колич.характеристики :15 с
Коллективы : Russian Science Foundation [14-26-00039]
Место публикации : Microb. Ecol.: SPRINGER, 2017. - Vol. 73, Is. 2. - С. 353-367. - ISSN 0095-3628, DOI 10.1007/s00248-016-0852-3. - ISSN 1432-184X(eISSN)
Примечания : Cited References:65. - This study was supported by the Russian Science Foundation (Grant No. 14-26-00039).
Предметные рубрики: TROPICAL COASTAL WATERS
PHA-DEGRADING BACTERIA
ALCALIGENES-EUTROPHUS
Ключевые слова (''Своб.индексиров.''): degradable polyhydroxyalkanoates--pha--soil--pha-degrading--microorganisms
Аннотация: The study addresses degradation of polyhydroxyalkanoates (PHA) with different chemical compositions-the polymer of 3-hydroxybutyric acid [P(3HB)] and copolymers of P(3HB) with 3-hydroxyvalerate [P(3HB/3HV)], 4-hydroxybutyrate [P(3HB/4HB)], and 3-hydroxyhexanoate [P(3HB/3HHx)] (10-12 mol%)-in the agro-transformed field soil of the temperate zone. Based on their degradation rates at 21 and 28 A degrees C, polymers can be ranked as follows: P(3HB/4HB) P(3HB/3HHx) P(3HB/3HV) P(3HB). The microbial community on the surface of the polymers differs from the microbial community of the soil with PHA specimens in the composition and percentages of species. Thirty-five isolates of bacteria of 16 genera were identified as PHA degraders by the clear zone technique, and each of the PHA had both specific and common degraders. P(3HB) was degraded by bacteria of the genera Mitsuaria, Chitinophaga, and Acidovorax, which were not among the degraders of the three other PHA types. Roseateles depolymerans, Streptomyces gardneri, and Cupriavidus sp. were specific degraders of P(3HB/4HB). Roseomonas massiliae and Delftia acidovorans degraded P(3HB/3HV), and Pseudoxanthomonas sp., Pseudomonas fluorescens, Ensifer adhaerens, and Bacillus pumilus were specific P(3HB/3HHx) degraders. All four PHA types were degraded by Streptomyces.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Prudnikova S.V., Karpov V.A., Ivonin V.N., D? N.L., Nguy?n T.H., Le T.M.H., Filichev N.L., Levin A.L., Filipenko M.L., Volova T.G., Gitelson I.I.
Заглавие : Microbial degradation of polyhydroxyalkanoates in tropical soils
Место публикации : International Biodeterioration and Biodegradation. - 2013. - Vol. 83. - С. 77-84. - ISSN 09648305 (ISSN) , DOI 10.1016/j.ibiod.2013.04.014
Ключевые слова (''Своб.индексиров.''): biopolymer properties--biopolymers--pha degrading microorganisms--polyhydroxyalkanoates (phas)--soil biodegradation--3-hydroxybutyric acid--degree of crystallinity--hydroxyalkanoic acids--microbial characteristics--microbial degradation--polyhydroxyalkanoates--polymer molecular mass--soil biodegradations--bacteriology--biomolecules--biopolymers--microorganisms--soils--tropics--biodegradation--biodegradation--chemical composition--microbial activity--microbial community--molecular analysis--organic compound--polymer--soil degradation--soil microorganism--tropical soil
Аннотация: The integrated study addressing biodegradation of microbial linear polyesters of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) in tropical conditions by microbial communities of Vietnamese soils was performed in locations close to Hanoi and Nha Trang, which differed in their weather conditions and microbial communities. It shows that PHA degradation in tropical soils is influenced by polymer chemical composition, specimen shape, and microbial characteristics. The homopolymer of 3-hydroxybutyric acid is degraded at higher rates than the copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids. The average rates of mass loss were 0.04-0.33% per day for films and 0.02-0.18% for compact pellets. PHA degradation was accompanied by a decrease in the polymer molecular mass and, usually, an increase in the degree of crystallinity, suggesting preferential degradation of the amorphous phase. Under the study conditions, representatives of the bacterial genera Burkholderia, Bacillus, Cupriavidus, Mycobacterium, and Nocardiopsis and such micromycetes as Acremonium, Gongronella, Paecilomyces, and Penicillium, Trichoderma have been identified as major PHA degraders. В© 2013 Elsevier Ltd.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Prudnikova S.V., Karpov V.A., Ivonin V.N., Do N.L., Nguyen T.H., Le TMH, Filichev N.L., Levin A.L., Filipenko M.L., Volova T.G., Gitelson I.I.
Заглавие : Microbial degradation of polyhydroxyalkanoates in tropical soils
Место публикации : Int. Biodeterior. Biodegrad.: ELSEVIER SCI LTD, 2013. - Vol. 83. - С. 77-84. - 8. - ISSN 0964-8305, DOI 10.1016/j.ibiod.2013.04.014
Примечания : Cited References: 74. - This study was financially supported by Project "Biotechnologies of novel biomaterials" (Agreement No. 11.G34.31.0013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning".
Предметные рубрики: POLY-BETA-HYDROXYBUTYRATE
CHAIN-LENGTH POLYHYDROXYALKANOATES
POLY(3-HYDROXYBUTYRATE) DEPOLYMERASE
COASTAL WATERS
PHA FILMS
BIODEGRADATION
BACTERIA
IDENTIFICATION
POLYESTERS
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates (phas)--biopolymers--biopolymer properties--soil biodegradation--pha degrading microorganisms
Аннотация: The integrated study addressing biodegradation of microbial linear polyesters of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) in tropical conditions by microbial communities of Vietnamese soils was performed in locations close to Hanoi and Nha Trang, which differed in their weather conditions and microbial communities. It shows that PHA degradation in tropical soils is influenced by polymer chemical composition, specimen shape, and microbial characteristics. The homopolymer of 3-hydroxybutyric acid is degraded at higher rates than the copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids. The average rates of mass loss were 0.04-0.33% per day for films and 0.02-0.18% for compact pellets. PHA degradation was accompanied by a decrease in the polymer molecular mass and, usually, an increase in the degree of crystallinity, suggesting preferential degradation of the amorphous phase. Under the study conditions, representatives of the bacterial genera Burkholderia, Bacillus, Cupriavidus, Mycobacterium, and Nocardiopsis and such micromycetes as Acremonium, Gongronella, Paecilomyces, and Penicillium, Trichoderma have been identified as major PHA degraders. (C) 2013 Elsevier Ltd. All rights reserved.
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