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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana G. G., Prudnikova, Svetlana V. V., Kiselev, Evgeniy G. G., Nemtsev, Ivan V. V., Vasiliev, Alexander D. D., Kuzmin, Andrey P. P., Shishatskaya, Ekaterina I. I.
Заглавие : Bacterial Cellulose (BC) and BC Composites: Production and Properties
Колич.характеристики :24 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Nanomaterials: MDPI, 2022. - Vol. 12, Is. 2. - Ст.192. - ISSN 2079-4991(eISSN), DOI 10.3390/nano12020192
Примечания : Cited References:113. - This research was financially supported by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006.
Предметные рубрики: SILVER NANOPARTICLES
GLUCONACETOBACTER-HANSENII
MICROBIAL CELLULOSE
Аннотация: The synthesis of bacterial cellulose (BC) by Komagataeibacter xylinus strain B-12068 was investigated on various C-substrates, under submerged conditions with stirring and in static surface cultures. We implemented the synthesis of BC on glycerol, glucose, beet molasses, sprat oil, and a mixture of glucose with sunflower oil. The most productive process was obtained during the production of inoculum in submerged culture and subsequent growth of large BC films (up to 0.2 m(2) and more) in a static surface culture. The highest productivity of the BC synthesis process was obtained with the growth of bacteria on molasses and glycerol, 1.20 and 1.45 g/L per day, respectively. We obtained BC composites with silver nanoparticles (BC/AgNPs) and antibacterial drugs (chlorhexidine, baneocin, cefotaxime, and doripenem), and investigated the structure, physicochemical, and mechanical properties of composites. The disc-diffusion method showed pronounced antibacterial activity of BC composites against E. coli ATCC 25922 and S. aureus ATCC 25923.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Yehia, Moustafa R., Smolyarova, Tatyana E., Shabanov, Alexandr V., Sushko, Ekaterina S., Badun, Gennady A., Kudryasheva, Nadezhda S.
Заглавие : Adaptation of a Bacterial Bioluminescent Assay to Monitor Bioeffects of Gold Nanoparticles
Колич.характеристики :14 с
Место публикации : Bioengineering-Basel: MDPI, 2022. - Vol. 9, Is. 2. - Ст.61. - ISSN 2306-5354(eISSN), DOI 10.3390/bioengineering9020061
Примечания : Cited References:91
Аннотация: Our current study aimed to adapt a bioluminescent bacteria-based bioassay to monitor the bioeffects of gold nanoparticles (AuNPs). Luminous marine bacteria Photobacterium phosphoreum and AuNPs modified with polyvinylpyrrolidone were employed; low-concentration (
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gusakov V. A., Makhutova O. N., Gladyshev M. I., Golovatyuk L. V., Zinchenko T. D.
Заглавие : Ecological role of cyprideis torosa and heterocypris salina (Crustacea, ostracoda) in saline rivers of the lake elton basin: Abundance, biomass, production, fatty acids
Место публикации : Zool. Stud.: Biodiversity Research Center, Academia Sinica, 2021. - Vol. 60. - С. 60-53. - ISSN 10215506 (ISSN), DOI 10.6620/ZS.2021.60-53
Аннотация: Saline rivers are highly productive ecosystems in arid regions. The meiobenthic community (bottom meiofauna) and its dominant representatives are one of the least studied components of these aquatic ecosystems. Ostracods Cyprideis torosa and Heterocypris salina are major consumers among the species of bottom meiofauna in saline rivers flowing into the hyperhaline Lake Elton (Volgograd Region, Russia). We estimated the abundance, biomass and production of C. torosa, the dominant species at the mouth of the polyhaline Chernavka River (average salinity is ~30 g l-1), and H. salina, the dominant species at the mouth of the mesohaline Bolshaya Samoroda River (~13 g l-1), in spring (May) and summer (August). Additionally, we studied the composition and content of fatty acids of the ostracods and their potential food sources (bottom sediments with bacterial-algal mats). We found that the abundance and biomass (wet weight with shells) of C. torosa in the Chernavka River and H. salina in the Bolshaya Samoroda River reached 3.5 ? 106 ind. m-2 and 117 g m-2, and 1.1 ? 105 ind. m-2 and 12 g m-2, respectively. The first species formed on average about 85% of the total abundance and 96% of the total biomass of the meiobenthos, and the second one, about 13% and 31%, respectively. The daily production of C. torosa and H. salina can reach 249 and 36 mg m-2 ash-free dry weight, respectively. The results indicate that these species may play an important role in the total flow of matter and energy in the studied habitats. Based on the fatty acid (FA) composition of the ostracods and their food sources, it was found that C. torosa mainly consumed diatoms, while H. salina preferred bacteria, cyanobacteria, and green algae. Differences between the species were greater than differences between the bottom sediments from the rivers. It may mean that the ostracods selectively consumed different food items that may be related to the different nutrient requirements of the species. Seasonal changes in the FA compositions of the ostracods were higher than in their food sources (bottom sediments), which also indicates selective feeding of the species. © 2021 Academia Sinica, Taiwan.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gusakov, Vladimir A., Makhutova, Olesia N., Gladyshev, Michail I., Golovatyuk L. V., Zinchenko, Tatiana D.
Заглавие : Ecological Role of Cyprideis torosa and Heterocypris salina (Crustacea, Ostracoda) in Saline Rivers of the Lake Elton Basin: Abundance, Biomass, Production, Fatty Acids
Колич.характеристики :16 с
Коллективы : Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [13-04-00740, 15-04-03341, 17-04-00135]; Russian FederationRussian Federation [51.1.1]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Zool. Stud.: BIODIVERSITY RESEARCH CENTER, ACAD SINICA, 2021. - Vol. 60. - Ст.53. - ISSN 1021-5506, DOI 10.6620/ZS.2021.60-53. - ISSN 1810-522X(eISSN)
Примечания : Cited References:84. - This research was performed in the framework of State Assignments No. 121051100109-1 and AAAA-A17-117112040039-7, and it was also supported by the grants from Russian Foundation for Basic Research (RFBR) (projects numbers 13-04-00740, 15-04-03341, 17-04-00135), State Assignment within the framework of the basic research program of the Russian Federation (topic No. 51.1.1), State Assignment of the Ministry of Science and Higher Education of the Russian Federation to Siberian Federal University in 2020 (project No. FSRZ-2020-0006 "Biologically active substances in environmental, biotechnological and medical systems"). The authors are grateful to Elena Krasova for linguistic check and improvements of the manuscript. We would also like to thank two anonymous reviewers for their constructive comments, recommendations as well as extra corrections of the paper's language and style.
Предметные рубрики: JONES 1850 CRUSTACEA
MACROZOOBENTHIC COMMUNITIES
INVERTEBRATES
Аннотация: Saline rivers are highly productive ecosystems in arid regions. The meiobenthic community (bottom meiofauna) and its dominant representatives are one of the least studied components of these aquatic ecosystems. Ostracods Cyprideis torosa and Heterocypris salina are major consumers among the species of bottom meiofauna in saline rivers flowing into the hyperhaline Lake Elton (Volgograd Region, Russia). We estimated the abundance, biomass and production of C. torosa, the dominant species at the mouth of the polyhaline Chernavka River (average salinity is similar to 30 g l(-1)), and H. salina, the dominant species at the mouth of the mesohaline Bolshaya Samoroda River (similar to 13 g l(-1)), in spring (May) and summer (August). Additionally, we studied the composition and content of fatty acids of the ostracods and their potential food sources (bottom sediments with bacterial-algal mats). We found that the abundance and biomass (wet weight with shells) of C. torosa in the Chernavka River and H. salina in the Bolshaya Samoroda River reached 3.5 x 10(6) ind. m(-2) and 117 g m(-2), and 1.1 x 10(5) ind. m(-2) and 12 g m(-2), respectively. The first species formed on average about 85% of the total abundance and 96% of the total biomass of the meiobenthos, and the second one, about 13% and 31%, respectively. The daily production of C. torosa and H. salina can reach 249 and 36 mg m(-2) ash-free dry weight, respectively. The results indicate that these species may play an important role in the total flow of matter and energy in the studied habitats. Based on the fatty acid (FA) composition of the ostracods and their food sources, it was found that C. torosa mainly consumed diatoms, while H. salina preferred bacteria, cyanobacteria, and green algae. Differences between the species were greater than differences between the bottom sediments from the rivers. It may mean that the ostracods selectively consumed different food items that may be related to the different nutrient requirements of the species. Seasonal changes in the FA compositions of the ostracods were higher than in their food sources (bottom sediments), which also indicates selective feeding of the species.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolosova, Elizaveta M., Sutormin, Oleg S., Stepanova L. V., Shpedt, Aleksandr A., Rimatskaya N. V., Sukovataya, Irina E., Kratasyuk, Valentina A.
Заглавие : Bioluminescent enzyme inhibition-based assay for the prediction of toxicity of pollutants in urban soils
Колич.характеристики :9 с
Коллективы : Russian Foundation for Basic Research, the Government of the Krasnoyarsk Region, Russia; Krasnoyarsk Regional Foundation for Supporting Scientific and Technological Activities, Russia [18-47-240005]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Environ. Technol. Innov.: ELSEVIER, 2021. - Vol. 24. - Ст.101842. - ISSN 2352-1864, DOI 10.1016/j.eti.2021.101842
Примечания : Cited References:46. - This work was supported by the Russian Foundation for Basic Research, the Government of the Krasnoyarsk Region, Russia, and Krasnoyarsk Regional Foundation for Supporting Scientific and Technological Activities, Russia [grant number 18-47-240005] in the field of statistical analysis and interpretation of the data; and the work related to the sample collection was supported by the Ministry of Science and Higher Education of the Russian Federation [grant number FSRZ-2020-0006].
Предметные рубрики: FLUORIDE
BIOASSAYS
POLLUTION
METALS
WATER
Аннотация: There is a need for rapid simple and informative environmental assessment methods. The present investigation is aimed at assessing the possibility of using the combined enzyme system of luminescent bacteria: NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc) for predicting the potential toxicity of industrial urbostratozems sampled in the city of Krasnoyarsk. Three groups of urbostratozems polluted with fluorine, arsenic and lead, were tested by the methods of chemical analysis and enzymatic bioassay. Only the assessment of the arsenic-contaminated soil samples showed the dependence between the reduced activity of the enzyme system and the arsenic concentration variations. The results reveal that the sensitivity of the Red + Luc enzyme system to the soil pollutants depends on the properties of the studied soil samples. Moreover, the solubility of lead in the soil samples affects the accuracy of the enzymatic bioassays for soil toxicity testing. The results of the enzymatic bioassay of the fluoride-contaminated soil samples are ambiguous. The obtained data show the relevance of the sample preparation during integral bioassays. In addition, soil properties should be taken into account as well. The current study emphasizes the importance of conducting chemical and biological testing as a combined set to obtain comprehensive information about the anthropogenic load. (C) 2021 Elsevier B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Wu Y. -T., Chiang P. -W., Tandon K., Rogozin D. Y., Degermendzhy A. G., Tang S. -L.
Заглавие : Single-cell genomics-based analysis reveals a vital ecological role of thiocapsa sp. LSW in the meromictic Lake Shunet, Siberia
Место публикации : Microb. Genomics: Microbiology Society, 2021. - Vol. 7, Is. 12. - Ст.000712. - ISSN 20575858 (ISSN), DOI 10.1099/mgen.0.000712
Аннотация: Meromictic lakes usually harbour certain prevailing anoxygenic phototrophic bacteria in their anoxic zone, such as the purple sulfur bacterium (PSB) Thiocapsa sp. LSW (hereafter LSW) in Lake Shunet, Siberia. PSBs have been suggested to play a vital role in carbon, nitrogen and sulfur cycling at the oxic–anoxic interface of stratified lakes; however, the ecological significance of PSBs in the lake remains poorly understood. In this study, we explored the potential ecological role of LSW using a deep-sequencing analysis of single-cell genomics associated with flow cytometry. An approximately 2.7 Mb draft genome was obtained based on the co-assembly of five single-cell genomes. LSW might grow photolithoautotrophically and could play putative roles not only as a carbon fixer and diazotroph, but also as a sulfate reducer/oxidizer in the lake. This study provides insights into the potential ecological role of Thiocapsa sp. in meromictic lakes. © 2021 The Authors.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S. V., Evgrafova S. Y., Volova T. G.
Заглавие : Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards “green” bioplastics
Место публикации : Chemosphere: Elsevier Ltd, 2021. - Vol. 263. - Ст.128180. - ISSN 00456535 (ISSN), DOI 10.1016/j.chemosphere.2020.128180
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic – poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9–11 °C, the total biomass of microorganisms at a 10-20-cm depth was 226–234 mg g?1 soil and CO2 production was 20–46 mg g?1 day?1. The total abundance of microscopic fungi varied between (7.4 ± 2.3) ? 103 and (18.3 ± 2.2) ? 103 CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 ± 0.1) ? 106 CFU g?1 soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique – plating of isolates on the mineral agar with polymer as sole carbon source – was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)-degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. © 2020 Elsevier Ltd
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolesnik, Olga V., Rozhko, Tatiana V., Lapina, Maria A., Solovyev, Vladislav S., Sachkova, Anna S., Kudryasheva, Nadezhda S.
Заглавие : Development of Cellular and Enzymatic Bioluminescent Assay Systems to Study Low-Dose Effects of Thorium
Колич.характеристики :13 с
Место публикации : Bioengineering-Basel: MDPI, 2021. - Vol. 8, Is. 12. - Ст.194. - ISSN 2306-5354(eISSN), DOI 10.3390/bioengineering8120194
Примечания : Cited References:77
Аннотация: Thorium is one of the most widespread radioactive elements in natural ecosystems, along with uranium, it is the most important source of nuclear energy. However, the effects of thorium on living organisms have not been thoroughly studied. Marine luminescent bacteria and their enzymes are optimal bioassays for studying low-dose thorium exposures. Luminescent bioassays provide a quantitative measure of toxicity and are characterized by high rates, sensitivity, and simplicity. It is known that the metabolic activity of bacteria is associated with the production of reactive oxygen species (ROS). We studied the effects of thorium-232 (10(-11)-10(-3) M) on Photobacterium phosphoreum and bacterial enzymatic reactions; kinetics of bacterial bioluminescence and ROS content were investigated in both systems. Bioluminescence activation was revealed under low-dose exposures (0.1 Gy) and discussed in terms of "radiation hormesis". The activation was accompanied by an intensification of the oxidation of a low-molecular reducer, NADH, during the enzymatic processes. Negative correlations were found between the intensity of bioluminescence and the content of ROS in bacteria and enzyme systems; an active role of ROS in the low-dose activation by thorium was discussed. The results contribute to radioecological potential of bioluminescence techniques adapted to study low-intensity radioactive exposures.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Wu, Yu-Ting, Chiang, Pei-Wen, Tandon, Kshitij, Rogozin, Denis Yu, Degermendzhy, Andrey G., Tang, Sen-Lin
Заглавие : Single- cell genomics-based analysis reveals a vital ecological role of Thiocapsa sp. LSW in the meromictic Lake Shunet, Siberia
Колич.характеристики :10 с
Коллективы : Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project [NSC102-2923-B-001-004-MY3]; Russian Foundation for Basic Research GrantRussian Foundation for Basic Research (RFBR) [21-54-52001]; MOST [105-2923-B-001-001-MY3]
Место публикации : Microb. Genomics: MICROBIOLOGY SOC, 2021. - Vol. 7, Is. 12. - Ст.000712. - ISSN 2057-5858, DOI 10.1099/mgen.0.000712
Примечания : Cited References:69. - This research was funded by the Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project Grant NSC102--2923-B--001--004--MY3 and by the Russian Foundation for Basic Research Grant (project no. 21--54--52001) and MOST (105--2923--B--001--001--MY3).
Предметные рубрики: PHOTOTROPHIC BACTERIAL COMMUNITY
PURPLE SULFUR BACTERIUM
SP NOV.
Аннотация: Meromictic lakes usually harbour certain prevailing anoxygenic phototrophic bacteria in their anoxic zone, such as the purple sulfur bacterium (PSB) Thiocapsa sp. LSW (hereafter LSW) in Lake Shunet, Siberia. PSBs have been suggested to play a vital role in carbon, nitrogen and sulfur cycling at the oxic-anoxic interface of stratified lakes; however, the ecological significance of PSBs in the lake remains poorly understood. In this study, we explored the potential ecological role of LSW using a deepsequencing analysis of single- cell genomics associated with flow cytometry. An approximately 2.7 Mb draft genome was obtained based on the co- assembly of five single- cell genomes. LSW might grow photolithoautotrophically and could play putative roles not only as a carbon fixer and diazotroph, but also as a sulfate reducer/oxidizer in the lake. This study provides insights into the potential ecological role of Thiocapsa sp. in meromictic lakes.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E., Nemtsev I., Lukyanenko A., Vasiliev A., Kiselev E., Sukovatyi A., Volova T.
Заглавие : Polymer Films of Poly-3-hydroxybutyrate Synthesized by Cupriavidus necator from Different Carbon Sources
Место публикации : J. Polym. Environ.: Springer, 2021. - Vol. 29, Is. 3. - С. 837-850. - ISSN 15662543 (ISSN), DOI 10.1007/s10924-020-01924-3
Аннотация: Films were prepared from 2% solutions of biodegradable poly-3-hydroxybutyrate [P(3HB)] and investigated. The polymer was synthesized by the Cupriavidus necator B-10646 bacterium cultivated using various carbon sources (glucose and glycerol of different degrees of purity, containing 0.3 to 17.93% impurities). Glycerol as the substrate influenced molecular-weight properties and crystallinity of the polymer without affecting its temperature characteristics. The P(3HB) specimens synthesized from glycerol had reduced Mw (300–400 kDa) and degree of crystallinity (50–55%) compared to the specimens synthesized from glucose (860 kDa and 76%, respectively). The low-crystallinity P(3HB) specimens, regardless of the degree of purity of glycerol, produced a beneficial effect on the properties of polymer films, which had a better developed folded surface and increased hydrophilicity. The values of the highest roughness (Ra) of the films synthesized from glycerol were 1.8 to 4.0 times lower and the water angles 1.4–1.6 times smaller compared to the films synthesized from glucose (71.75 nm and 87.4°, respectively). Those films performed better as cell scaffolds: the number of viable NIH fibroblasts was 1.7–1.9 times higher than on polystyrene (control) or films of P(3HB) synthesized from glucose. © 2020, Springer Science+Business Media, LLC, part of Springer Nature.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondareva, Lydia, Kudryasheva, Nadezhda
Заглавие : Direct and Indirect Detoxification Effects of Humic Substances
Колич.характеристики :13 с
Коллективы : Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Russian Federation) 2020-2025
Место публикации : Agronomy-Basel: MDPI, 2021. - Vol. 11, Is. 2. - Ст.198. - ISSN 2073-4395(eISSN), DOI 10.3390/agronomy11020198
Примечания : Cited References:79. - This review was prepared with the partial financial support of the Program of the Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (Russian Federation) 2020-2025.
Аннотация: The review summarizes studies on the detoxification effects of water-soluble humic substances (HS), which are products of the natural transformation of organic substances in soils and bottom sediments that serve as natural detoxifying agents in water solutions. The detoxifying effects of HS on microorganisms are quite complex: HS neutralize free pollutants (indirect bioeffects) and also stimulate the protective response of organisms (direct bioeffects). Prospects and potential problems of bioluminescent bacteria-based assay to monitor toxicity of solutions in the presence of HS are discussed. The main criterion for the bioassay application is versatility and ease of use. The detoxification efficiency of HS in different pollutant solutions was evaluated, and the detoxification mechanisms are discussed. Particular attention was paid to the direct and complex direct + indirect effects of HS. The review focuses on the protective function of HS in solutions of radionuclides and salts of stable metals, with special consideration of the antioxidant properties of HS.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova, Svetlana, V, Evgrafova, Svetlana Yu, Volova, Tatiana G.
Заглавие : Metabolic activity of cryogenic soils in the subarctic zone of Siberia towards "green" bioplastics
Колич.характеристики :12 с
Коллективы : Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]; Government of the Russian Federation [220]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Chemosphere: PERGAMON-ELSEVIER SCIENCE LTD, 2021. - Vol. 263. - Ст.128180. - ISSN 0045-6535, DOI 10.1016/j.chemosphere.2020.128180. - ISSN 1879-1298(eISSN)
Примечания : Cited References:101. - This study (polymer synthesis and investigation) was financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning", and by the State assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (investigation of polymer degradation in soils of Evenkia).
Аннотация: The present study investigates, for the first time, the structure of the microbial community of cryogenic soils in the subarctic region of Siberia and the ability of the soil microbial community to metabolize degradable microbial bioplastic - poly-3-hydroxybutyrate [P(3HB)]. When the soil thawed, with the soil temperature between 5-7 and 9-11 degrees C, the total biomass of microorganisms at a 10-20-cm depth was 226-234 mg g(-1) soil and CO2 production was 20-46 mg g(-1)W day(-1). The total abundance of microscopic fungi varied between (7.4 +/- 2.3) x 10(3) and (18.3 +/- 2.2) x 10(3) CFU/g soil depending on temperature; the abundance of bacteria was several orders of magnitude greater: (1.6 +/- 0.1) x 10(6) CFU g(-1) soil. The microbial community in the biofilm formed on the surface of P(3HB) films differed from the background soil in concentrations and composition of microorganisms. The activity of microorganisms caused changes in the surface microstructure of polymer films, a decrease in molecular weight, and an increase in the degree of crystallinity of P(3HB), indicating polymer biodegradation due to metabolic activity of microorganisms. The clear-zone technique e plating of isolates on the mineral agar with polymer as sole carbon source e was used to identify P(3HB)-degrading microorganisms inhabiting cryogenic soil in Evenkia. Analysis of nucleotide sequences of rRNA genes was performed to identify the following P(3HB)degrading species: Bacillus pumilus, Paraburkholderia sp., Pseudomonas sp., Rhodococcus sp., Stenotrophomonas rhizophila, Streptomyces prunicolor, and Variovorax paradoxus bacteria and the Penicillium thomii, P. arenicola, P. lanosum, Aspergillus fumigatus, and A. niger fungi. (C) 2020 Elsevier Ltd. All rights reserved.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Neubauer, Darshan, Kolmakova, Olesya, Woodhouse, Jason, Taube, Robert, Mangelsdorf, Kai, Gladyshev, Michail, Premke, Katrin, Grossart, Hans-Peter
Заглавие : Zooplankton carcasses stimulate microbial turnover of allochthonous particulate organic matter
Колич.характеристики :16 с
Коллективы : Projekt DEAL
Место публикации : ISME J.: SPRINGERNATURE, 2021. - Article in press. - ISSN 1751-7362, DOI 10.1038/s41396-020-00883-w. - ISSN 1751-7370(eISSN)
Примечания : Cited References:83. - Open Access funding enabled and organized by Projekt DEAL.
Предметные рубрики: FRESH-WATER
SEASONAL-CHANGES
CARBON
LAKE
DECOMPOSITION
DEGRADATION
Аннотация: Carbon turnover in aquatic environments is dependent on biochemical properties of organic matter (OM) and its degradability by the surrounding microbial community. Non-additive interactive effects represent a mechanism where the degradation of biochemically persistent OM is stimulated by the provision of bioavailable OM to the degrading microbial community. Whilst this is well established in terrestrial systems, whether it occurs in aquatic ecosystems remains subject to debate. We hypothesised that OM from zooplankton carcasses can stimulate the degradation of biochemically persistent leaf material, and that this effect is influenced by the daphnia:leaf OM ratio and the complexity of the degrading microbial community. Fresh Daphnia magna carcasses and C-13-labelled maize leaves (Zea mays) were incubated at different ratios (1:1, 1:3 and 1:5) alongside either a complex microbial community (50 mu m) or solely bacteria (0.8 mu m). C-13 stable-isotope measurements of CO2 analyses were combined with phospholipid fatty acids (PLFA) analysis and DNA sequencing to link metabolic activities, biomass and taxonomic composition of the microbial community. Our experiments indicated a significantly higher respiration of leaf-derived C when daphnia-derived OM was most abundant (i.e. daphnia:leaf OM ratio of 1:1). This process was stronger in a complex microbial community, including eukaryotic microorganisms, than a solely bacterial community. We concluded that non-additive interactive effects were a function of increased C-N chemodiversity and microbial complexity, with the highest net respiration to be expected when chemodiversity is high and the degrading community complex. This study indicates that identifying the interactions and processes of OM degradation is one important key for a deeper understanding of aquatic and thus global carbon cycle.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chen, Yu-Hsiang, Chiang, Pei-Wen, Rogozin, Denis Yu, Degermendzhy, Andrey G., Chiu, Hsiu-Hui, Tang, Sen-Lin
Заглавие : Salvaging high-quality genomes of microbial species from a meromictic lake using a hybrid sequencing approach
Колич.характеристики :12 с
Коллективы : Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project [NSC 102-2923-B-001-004-MY3]; Russian Foundation for Basic Research GrantRussian Foundation for Basic Research (RFBR) [21-54-52001, MOST 105-2923-B-001-001-MY3]; Taiwan International Graduate Program (TIGP)
Место публикации : Commun. Biol.: NATURE PORTFOLIO, 2021. - Vol. 4, Is. 1. - Ст.996. - ISSN 2399-3642(eISSN), DOI 10.1038/s42003-021-02510-6
Примечания : Cited References:99. - Y This work was supported by the Ministry of Science and Technology in Taiwan through the Taiwan-Russia Joint Project Grant NSC 102-2923-B-001-004-MY3 and Russian Foundation for Basic Research Grant 21-54-52001 and MOST 105-2923-B-001-001-MY3. Y.H.C. would like to acknowledge the Taiwan International Graduate Program (TIGP) for its fellowship towards his graduate studies. We would like to thank Noah Last of Third Draft Editing for his English language editing.
Предметные рубрики: DIMETHYL SULFIDE
SULFUR BACTERIUM
SP-NOV.
CHLAMYDIALES
METAGENOME
Аннотация: Most of Earth's bacteria have yet to be cultivated. The metabolic and functional potentials of these uncultivated microorganisms thus remain mysterious, and the metagenome-assembled genome (MAG) approach is the most robust method for uncovering these potentials. However, MAGs discovered by conventional metagenomic assembly and binning are usually highly fragmented genomes with heterogeneous sequence contamination. In this study, we combined Illumina and Nanopore data to develop a new workflow to reconstruct 233 MAGs-six novel bacterial orders, 20 families, 66 genera, and 154 species-from Lake Shunet, a secluded meromictic lake in Siberia. With our workflow, the average N50 of reconstructed MAGs greatly increased 10-40-fold compared to when the conventional Illumina assembly and binning method were used. More importantly, six complete MAGs were recovered from our datasets. The recovery of 154 novel species MAGs from a rarely explored lake greatly expands the current bacterial genome encyclopedia. Chen and colleagues develop a workflow for assembling high quality metagenome-associated genomes for microbial species using long and short reads, in this case from a meromictic lake. A full, detailed workflow is provided in for use by the community.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolosova E. M., Sutormin O. S., Stepanova L. V., Shpedt A. A., Rimatskaya N. V., Sukovataya I. E., Kratasyuk V. A.
Заглавие : Bioluminescent enzyme inhibition-based assay for the prediction of toxicity of pollutants in urban soils
Место публикации : Environ. Technol. Innov.: Elsevier B.V., 2021. - Vol. 24. - Ст.101842. - ISSN 23521864 (ISSN), DOI 10.1016/j.eti.2021.101842
Аннотация: There is a need for rapid simple and informative environmental assessment methods. The present investigation is aimed at assessing the possibility of using the combined enzyme system of luminescent bacteria: NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc) for predicting the potential toxicity of industrial urbostratozems sampled in the city of Krasnoyarsk. Three groups of urbostratozems polluted with fluorine, arsenic and lead, were tested by the methods of chemical analysis and enzymatic bioassay. Only the assessment of the arsenic-contaminated soil samples showed the dependence between the reduced activity of the enzyme system and the arsenic concentration variations. The results reveal that the sensitivity of the Red + Luc enzyme system to the soil pollutants depends on the properties of the studied soil samples. Moreover, the solubility of lead in the soil samples affects the accuracy of the enzymatic bioassays for soil toxicity testing. The results of the enzymatic bioassay of the fluoride-contaminated soil samples are ambiguous. The obtained data show the relevance of the sample preparation during integral bioassays. In addition, soil properties should be taken into account as well. The current study emphasizes the importance of conducting chemical and biological testing as a combined set to obtain comprehensive information about the anthropogenic load. © 2021 Elsevier B.V.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakova O. V., Trusova, M. Yu., Baturina O. A., Kabilov M. R.
Заглавие : Bacteria of Lake Pyasino and Adjacent Rivers after an Accidental Diesel Spill in 2020
Колич.характеристики :12 с
Коллективы : Siberian Branch of the Russian Academy of SciencesRussian Academy of Sciences [223-EP-2020/07]; Project Office for the Development of the Arctic (PORA); State Task of the Fundamental Research Program of the Russian Federation [51.1.1]; ICBFM SB RAS [AAAAA17-117020210021-7]
Место публикации : Contemp. Probl. Ecol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 14, Is. 4. - С. 356-367. - ISSN 1995-4255, DOI 10.1134/S1995425521040053. - ISSN 1995-4263(eISSN)
Примечания : Cited References:22. - This work was supported by economic agreement no. 223-EP-2020/07 with the Siberian Branch of the Russian Academy of Sciences and the Project Office for the Development of the Arctic (PORA), and State Task of the Fundamental Research Program of the Russian Federation, topic no. 51.1.1. Bioinformatics analysis was supported by basic budget funding from the ICBFM SB RAS (AAAAA17-117020210021-7).
Предметные рубрики: SP-NOV.
GEN. NOV.
OIL
BACTERIOPLANKTON
SEQUENCES
Аннотация: The bacterial composition of water and sediments in Lake Pyasino and adjacent rivers has been studied by high-throughput sequencing for the first time. No hydrocarbon-degrading bacteria are found in the river sections exposed to the diesel spillage in May 2020. The Daldykan and Ambarnaya rivers, located downstream of the spill site, are dominated by bacteria that oxidize sulfur and heavy metals. At the same time, these bacteria are not found in Lake Pyasino, which is dominated by small cyanobacteria. Thus, the composition of bacteria in the water and sediments of the Norilsk-Pyasino lake-river system corresponds to the technogenic impact to which it is exposed and also demonstrates the buffer properties of Lake Pyasino as water flows into the Kara Sea.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rogozin D. Y., Zykov V. V., Bulkhin A. O., Degermendzhi A. G.
Заглавие : Okenone in Bottom Sediments as a Proxy for Changes in the Water Level of a Saline Stratified Lake
Колич.характеристики :4 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-05-00428]
Место публикации : Dokl. Earth Sci.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2020. - Vol. 493, Is. 1. - С. 565-568. - ISSN 1028-334X, DOI 10.1134/S1028334X20070168. - ISSN 1531-8354(eISSN)
Примечания : Cited References:8. - This work was supported by the Russian Foundation for Basic Research, project no. 19-05-00428.
Предметные рубрики: SHIRA
SIBERIA
CYCLES
Аннотация: Long-term measurements show that the sedimentation dynamics of okenone (a carotenoid of purple sulfur bacteria) reflects the abundance of purple sulfur bacteria, the hydrogen sulfide content, and the stratification stability in the closed saline meromictic Shira Lake (Southern Siberia, Russia). The profile of fossil okenone in bottom sediments has been compared with the data on the water surface level in the lake over the last 100 years. The peaks of okenone correspond to lake transgressions, and the drops in the okenone content coincide with periods of stable or decreasing water level. Our observations show that fossil okenone may be used as a qualitative proxy of meromictic conditions and as a quantitative proxy of the water level dynamics in closed stratified lakes. Therefore, okenone can be used for qualitative reconstructions of the water balance and climate humidity in arid zones.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chan, Ya-Fan, Chiang, Pei-Wen, Tandon, Kshitij, Rogozin, Denis, Degermendzhi, Andrey, Zykov, Vladimir, Tang, Sen-Lin
Заглавие : Spatiotemporal Changes in the Bacterial Community of the Meromictic Lake Uchum, Siberia
Колич.характеристики :13 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-05-00428]; Russian Foundation for Basic Research, Government of Krasnoyarsk Territory; Krasnoyarsk Regional Fund of Science (project: "Bottom sediments of Lake Uchum (Krasnoyarsky kray) as a source of information for the reconstruction of the paleo-climate and the prediction of the healing properties of the lake") [18-45-243002]; Ministry of Science and Technology, TaiwanMinistry of Science and Technology, Taiwan [105-2923-B-001-001-MY3, 108-2811-M-001-603]
Место публикации : Microb. Ecol.: SPRINGER, 2020. - Article in press. - ISSN 0095-3628, DOI 10.1007/s00248-020-01592-9. - ISSN 1432-184X(eISSN)
Примечания : Cited References:77. - This work was supported by the Russian Foundation for Basic Research (grant no. 19-05-00428) and the Russian Foundation for Basic Research, Government of Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science (project: "Bottom sediments of Lake Uchum (Krasnoyarsky kray) as a source of information for the reconstruction of the paleo-climate and the prediction of the healing properties of the lake", grant no. 18-45-243002) and the Ministry of Science and Technology, Taiwan (project: "Transition in microbial community of stratified lakes in arid zone of South Siberia: Current and Past," grant no. 105-2923-B-001-001-MY3 and project: "Postdoctoral Research Fellows", grant no. 108-2811-M-001-603).
Предметные рубрики: PHOTOTROPHIC SULFUR BACTERIA
SP NOV.
MICROBIAL COMMUNITIES
Аннотация: Lake Uchum is a newly defined meromictic lake in Siberia with clear seasonal changes in its mixolimnion. This study characterized the temporal dynamics and vertical profile of bacterial communities in oxic and anoxic zones of the lake across all four seasons: October (autumn), March (winter), May (spring), and August (summer). Bacterial richness and diversity in the anoxic zone varied widely between time points.Proteobacteriawas the dominant bacterial phylum throughout the oxic and anoxic zones across all four seasons.Alphaproteobacteria(Loktanella) andGammaproteobacteria(Aliidiomarina) exhibited the highest abundance in the oxic and anoxic zone, respectively. Furthermore, there was a successional shift in sulfate-reducing bacteria (SRB) and sulfur-oxidizing bacteria in the anoxic zone across the seasons. The most dominant SRB,Desulfonatronovibriosp., is likely one of the main producers of hydrogen sulfide (H2S) and typically accumulates the most H2S in winter. The representative anoxygenic phototrophic bacterial group in Lake Uchum was purple sulfur bacteria (PSB). PSB were dominant (60.76%) in summer, but only had 0.2-1.5% relative abundance from autumn to spring. Multivariate analysis revealed that the abundance of these SRB and PSB correlated to the concentration of H2S in Lake Uchum. Taken together, this study provides insights into the relationships between changes in bacterial community and environmental features in Lake Uchum.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Esimbekova E. N., Govorun A. E., Lonshakova-Mukina V. I., Kratasyuk V. A.
Заглавие : Gelatin and Starch: What Better Stabilizes the Enzyme Activity?
Место публикации : Dokl. Biol. Sci.: Pleiades Publishing, 2020. - Vol. 491, Is. 1. - С. 43-46. - ISSN 00124966 (ISSN), DOI 10.1134/S0012496620020039
Аннотация: Abstract: The regularities of the functioning of a number of enzymes in a viscous environment created by natural polymers, starch and gelatin are examined. Based on the analysis of kinetic curves of thermal inactivation, mechanisms of thermal inactivation of enzymes in a viscous microenvironment are proposed. Using the example of butyrylcholinesterase, NAD(P)H:FMN oxidoreductase, and coupled system of the luminous bacteria (NAD(P)H:FMN oxidoreductase + luciferase), the conditions, under which starch and gelatin have a stabilizing effect on enzyme activity during storage and exposure to various physical and chemical environmental factors, were found. A significant increase in the stabilizing effect is achieved by eliminating water during drying the enzyme preparations immobilized in starch and gelatin polymer gels. © 2020, Pleiades Publishing, Ltd.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondarenko L. S., Kovel E. S., Kydralieva K. A., Dzhardimalieva G. I., Illes E., Tombacz E., Kicheeva A. G., Kudryasheva N. S.
Заглавие : Effects of modified magnetite nanoparticles on bacterial cells and enzyme reactions
Место публикации : Nanomaterials: MDPI AG, 2020. - Vol. 10, Is. 8. - Ст.1499. - С. 1-20. - ISSN 20794991 (ISSN), DOI 10.3390/nano10081499
Аннотация: Current paper presents biological effects of magnetite nanoparticles (MNPs). Analyzing effects of MNP’ characteristics (zeta-potential and hydrodynamic diameters) on bacteria and their enzyme reactions was the main focus. Photobacterium phosphoreum and bacterial enzymatic reactions were chosen as bioassays. Three types of MNPs were under study: bare Fe3O4, Fe3O4 modified with 3-aminopropyltriethoxysilane (Fe3O4/APTES), and humic acids (Fe3O4/HA). Effects of the MNPs were studied at a low concentration range (< 2 mg/L) and attributed to availability and oxidative activity of Fe3+, high negative surface charge, and low hydrodynamic diameter of Fe3O4/HA, as well as higher Fe3+ content in suspensions of Fe3O4/HA. Low-concentration suspensions of bare Fe3O4 provided inhibitory effects in both bacterial and enzymatic bioassays, whereas the MNPs with modified surface (Fe3O4/APTES and Fe3O4/HA) did not affect the enzymatic activity. Under oxidative stress (i.e., in the solutions of model oxidizer, 1,4-benzoquinone), MNPs did not reveal antioxidant activity, moreover, Fe3O4/HA demonstrated additional inhibitory activity. The study contributes to the deeper understanding of a role of humic substances and silica in biogeochemical cycling of iron. Bioluminescence assays, cellular and enzymatic, can serve as convenient tools to evaluate bioavailability of Fe3+ in natural dispersions of iron-containing nanoparticles, e.g., magnetite, ferrihydrite, etc. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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