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1.

Вид документа : Однотомное издание
Шифр издания : Е071/Б 63
Автор(ы) : Гительзон, Иосиф Исаевич, Волова, Татьяна Григорьевна, Дегерменджи, Андрей Георгиевич, Дегерменджи Н. Н., Шевырногов, Анатолий Петрович, Кратасюк В. А., Барцев, Сергей иванович, Болсуновский, Александр Яковлевич, Бондарь, Владимир Антонович, Буров А. Е., Величко В. В., Гладышев, Михаил Иванович, Есимбекова Е. Н., Дементьев Д. В., Есимбекова Е. Н., Задереев, Егор Сергеевич, Зотина Т. А., Косиненко, Сергей Васильевич, Медведева С. Е., Петушков В. Н., Печуркин, Николай Савельевич, Прокопкин И. Г., Пузырь А. П., Пуртов К. В., Рогозин, Денис Юрьевич, Родионова Н. С., Ронжин Н. О., Сомова, Лидия Александровна, Тихомиров, Александр Аполлинариевич, Тихомирова, Наталья Александровна, Трифонов С. В., Ушакова, Софья Аврумовна, Франк Л. А., Хромечек Е. Б., Шишацкая Е. И., Шуваев А. Н.
Заглавие : Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона
Выходные данные : Новосибирск: Издательство Сибирского отделения Российской академии наук, 2019
Колич.характеристики :292, [2] с.: ил., цв. ил.; 25 см.
Коллективы : Российская академия наук. Сибирское отделение, Институт биофизики (Красноярск)
Примечания : Рез. ст. англ. - Библиогр. в конце ст.
ISBN, Цена 978-5-7692-1650-3: 1635.00 р.
ГРНТИ : 31.27 + 76.03
УДК : 577 + 574 + 61
ББК : Е071я43 + Р252.0я43
Предметные рубрики: Экологическая биофизика
Медицинская биофизика
Ключевые слова (''Своб.индексиров.''): биолюминесценция--люцифераза--целентаразин--олигохеты--замкнутые экосистемы--управляемый биосинтез--наноалмазы--радиоизотопные методы--биосфера--жирные кислоты--системы жизнеобеспечения--меромиктические озера--геобиосфера--эволюция--глобальный климат--медицинская биофизика
Содержание : Краткий очерк истории, состояния и перспектив/ И. И. Гительзон. Коллекция культур ибсо как база для исследований биолюминесценции й и грибов в ИБФ СО РАН/ С. Е. Медведева. Биолюминесценция Мирового океана/ И. И. Гительзон, Л. А. Левин, А. С. Артемкин, Р. Н., Чепилов В. В., Молвинских С.Л., Черепанов О. А., Чугунов Ю. В., Караев Н. Д., Загородний Ю. А., Шевырногов А. П. Утюшев Р. Н. Бактериальная люцифераза в биолюминесцентном анализе/ В. А. Кратасюк, Е. Н. Есимбекова. Целентеразин-зависимые биолюминесцентные системы/ Л. А. Франк. Изучение химического механизма биолюминесценции грибов/ К. В. Пуртов, В. Н. Петушков, Н. С. Родионова. Исследование биолюминесценции сибирских почвенных олигохет/ Н. С. Родионова, А. А. Петушков. Экспериментальные модели замкнутых экосистем с расчетной долей человека как перспективное направление исследований по созданию биолого-технической системы жизнеобеспечения/ А. А. Тихомиров, С. А. Ушакова, Н. А. Тихомирова, С. В., Величко В. В. Трифонов С. В. Управляемый биосинтез: от параметрически управляемых продуцирующих биосистем до новейших биофизических технологий/ Т. Г. Волова, Е. И. Шишацкая. Биомедицинские приложения наноалмазов взрывного синтеза/ В. С. Бондарь, А. П. Пузырь, Н. О. Ронжин, А. В., Буров А. Е. Барон А. В. Применение радиоизотопных методов в институте биофизики СО РАН: от клеток крови до экосистем/ А. Я. Болсуновский, С. В. Косиненко, Т. А. Зотина, Д. В. Дементьев. Биосфера - взгляд сверху (экспрессные методы мониторинга биосферы в ИБФ СО РАН – ХХ–ХХI вв.)/ А. П. Шевырногов. Жирные кислоты в экологической биофизике водных систем/ М. И. Гладышев. Сравнительное исследование устойчивости стратификации и структуры трофической сети в меромиктических озерах Шира и Шунет (Южная Сибирь, Россия)/ Д. Ю. Рогозин, Е. С. Задереев, И. Г. Прокопкин [и др.]. Непрерывный рост интенсивности энерго-вещественных взаимодействий в эволюции геобиосферы Земли/ Н. С. Печуркин, А. Н. Шуваев, Л. А. Сомова. Малоразмерные модели биосферы и феноменология изменения глобального климата/ С. И. Барцев, А. Г. Дегерменджи. Направления развития биофизики в Красноярске/ А. Г. Дегерменджи.
Аннотация: Сборник посвящен широкому кругу исследований в области экологической биофизики – научного направления на стыке наук – от исследований на молекулярном уровне до вопросов управления большими природными экосистемами. Рассмотрены исторические вехи развития экологического направления биофизики. Основной акцент сборника основан на современных, актуальных достижениях красноярских биофизиков, которым удалось сохранить и развить многоплановые направления, которые были заложены в 50-х гг. ХХ века И. И. Гительзоном. Наряду с обзорными материалами и результатами фундаментальных исследований представлен ряд готовых к внедрению биотехнологий. Книга адресована биофизикам, экологам и химикам, а также преподавателям и студентам биофизических, биологических и экологических кафедр университетов.
Экземпляры :ИБФ-КФ(1)
Свободны : ИБФ-КФ(1)
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2.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tirranen L.S., Borodina E.V., Ushakova S.A., Rygalov V.Y., Gitelson J.I.
Заглавие : Effect of volatile metabolites of dill, radish and garlic on growth of bacteria
Колич.характеристики :4 с
Место публикации : Acta Astronaut.: PERGAMON-ELSEVIER SCIENCE LTD, 2001. - Vol. 49, Is. 2. - P105-108. - ISSN 0094-5765, DOI 10.1016/S0094-5765(01)00006-6
Примечания : Cited References: 2
Аннотация: In a model experiment plants were grown in sealed chambers on expanded clay aggregate under the luminance of 150 W/m(2) PAR and the temperature of 24 degreesC. Seven bacterial strains under investigation, replicated on nutrient medium surface in Petri dishes, were grown in the atmosphere of cultivated plants. Microbial response was evaluated by the difference between colony size in experiment and in control. In control, bacteria grew in the atmosphere of clean air. To study the effect of volatile metabolites of various plant on microbial growth, the experimental data were compared with the background values defined for each individual experiment. Expanded clay aggregate, luminance, temperature and sealed chamber (without plants) for the background were the same. Volatile metabolites from 28-days old radish plants have been reliably established to have no effect on the growth of microbes under investigation. Metabolites of 30-days old dill and 50-days old garlic have been established to have reliable bacteriostatic effect on the growth of three bacterial strains. Dill and garlic have been found to have different range of effects of volatile substances on bacterial growth. Volatile metabolites of dill and garlic differed in their effect on the sensitivity spectrum of bacteria. An attempt has been made to describe the obtained data mathematically. (C) 2001 International Astronautical Federation. Published by Elsevier Science Ltd.
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3.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sukovataya I.E., Tyulkova N.A.
Заглавие : Kinetic analysis of bacterial water-organic media
Колич.характеристики :3 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 2001. - Vol. 16: 11th International Bioluminescence and Chemiluminescence Symposium (SEP 06-10, 2000, PACIFIC GROVE, CALIFORNIA), Is. 4. - P271-273. - ISSN 1522-7235, DOI 10.1002/bio.649.abs
Примечания : Cited References: 10
Ключевые слова (''Своб.индексиров.''): bacterial luciferase--organic solvents--michaelis constant
Аннотация: The interaction of luciferases from two types of luminous bacteria, Photobacterium leiognathi and Vibrio harveyi, with their substrates [the photorecovered. FMNH2 and long-chain aldehydes-decanal (C-10), dodecanal (C-12) and tetradecanal (C-14)] in water-organic media was analysed using kinetic graphical methods. Moderate concentrations of organic solvents have been demonstrated to activate the bioluminescence, while higher concentrations inhibit it. The interactions of these effectors with luciferases show different types of kinetics, which depend on concentrations of solvents, kinds of enzymes and substrates. The apparent value of the Michaelis constant, K-m for C-14 of both luciferases and for C-10 of luciferase V. harveyi is enhanced with increasing concentration of the organic solvent, but K-m for C-12 and C-10 of luciferase P. leiognathi decreases. Obviously, at the specific binding of aldehydes with luciferases in the first case, hydrophobic interactions are realized, but in second, the electrostatic interactions are realized. The series of changes in parameters of bioluminescence reaction catalysed by different luciferases is obviously determined by their structural peculiarities. Copyright (C) 2001 John Wiley & Sons, Ltd.
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4.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tirranen L.S.
Заглавие : Formation of higher plant component microbial community in closed ecological system
Колич.характеристики :6 с
Место публикации : Acta Astronaut.: PERGAMON-ELSEVIER SCIENCE LTD, 2001. - Vol. 49, Is. 1. - P47-52. - ISSN 0094-5765, DOI 10.1016/S0094-5765(01)00005-4
Примечания : Cited References: 13
Аннотация: Closed ecological systems (CES) place at the disposal of a researcher unique possibilities to study the role of microbial communities in individual components and of the entire system. The microbial community of the higher plant component has been found to form depending on specific conditions of the closed ecosystem: length of time the solution is reused, introduction of intrasystem waste water into the nutrient medium, effect of other component of the system, and system closure in terms of gas exchange. The higher plant component formed its own microbial complex different from that formed prior to closure. The microbial complex of vegetable polyculture is more diverse and stable than the monoculture of wheat. The composition of the components' microflora changed, species diversity decreased, individual species of bacteria and fungi whose numbers were not so great before the closure prevailed. Special attention should be paid to phytopathogenic and conditionally pathogenic species of microorganisms potentially hazardous to man or plants and the least controlled in CES. This situation can endanger creation of CES and make conjectural existence of preplanned components, man, specifically, and consequently, of CES as it is. (C) 2001 International Astronautical Federation. Published by Elsevier Science Ltd.
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5.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide
Колич.характеристики :7 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 70, Is. 6. - P640-646. - ISSN 0026-2617, DOI 10.1023/A:1013175413013
Примечания : Cited References: 19
Ключевые слова (''Своб.индексиров.''): hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis--co inhibition
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-beta-ketothiolase. acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % beta-hydroxybutyrate and trace amounts of beta-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.
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6.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Popova L.Y., Maksimova E.E., Lobova T.I., Kargatova T.V., Boyandin A.N., Krylova T.Y., Pechurkin N.S.
Заглавие : Stability of recombinant plasmids in transgenic microorganisms under different environmental conditions
Колич.характеристики :7 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 70, Is. 6. - P685-691. - ISSN 0026-2617, DOI 10.1023/A:1013187815739
Примечания : Cited References: 15
Предметные рубрики: BACTERIA
Ключевые слова (''Своб.индексиров.''): recombinant plasmids--transgenic microorganisms--antibiotic resistance--bioluminescence--interferon
Аннотация: The copy number of R plasmids weakly depends on the selective pressure of the respective antibiotic but does depend on the physiology of the host species and the type of plasmids and cloned genes, whose expression leads to a further load on the biosynthetic apparatus of cells. The last factor is critical in the maintenance of recombinant plasmids in transgenic microorganisms.
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7.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr' A.P., Mogil'naya O.A., Gurevich Y.L., Babkina E.A.
Заглавие : Colony structure of a consortium of nitrifying bacteria
Колич.характеристики :7 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 70, Is. 1. - P84-90. - ISSN 0026-2617, DOI 10.1023/A:1004853123326
Примечания : Cited References: 6
Предметные рубрики: COMMUNITY
Ключевые слова (''Своб.индексиров.''): electron microscopy--colony architectonics--nitrifying bacteria
Аннотация: Colonies produced by a consortium of nitrifying bacteria were studied using fight and electron microscopy. The colonies were obtained by direct plating of inoculum from a two-stage nonsterile chemostat fermenter and by repeatedly passing the microbial community of the fermenter through selective media containing ammonium or nitrite. The colonies studied can be characterized by a specific combination of six types of cells differing in their ultrastructure and spatial location within the colony. The types of cells occurring within a given colony were found to depend on the nitrogen compound present in the medium. As a result of our study, morphological features of colonial bacterial communities were identified. The proposed approach can be viewed as a method to describe microbial associations and communities.
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8.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Y.
Заглавие : Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems
Колич.характеристики :6 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH: ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P1581-1586. - ISBN 0273-1177, DOI 10.1016/S0273-1177(01)00249-6
Примечания : Cited References: 17
Предметные рубрики: MICROCOSMS
BACTERIA
Аннотация: Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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9.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kargatova T.V., Maksimova E.E., Popova L.Y.
Заглавие : Coexistence of genetically engineered Escherichia coli strains and natural microorganisms in experimental aquatic microcosms
Колич.характеристики :6 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 70, Is. 2. - P211-216. - ISSN 0026-2617, DOI 10.1023/A:1010437731920
Примечания : Cited References: 17
Предметные рубрики: SURVIVAL
BACTERIA
GROWTH
Ключевые слова (''Своб.индексиров.''): escherichia coli--aquatic microecosystems--recombinant plasmid
Аннотация: In experimental aquatic microcosms (AMCs), the population of the Escherichia coli strain Z905 harboring the recombinant plasmid pPHL7 (Ap(r)Lux(+)) was found to gradually accumulate AMC-adapted cells, which retained the plasmid but differed from the original cells in some biochemical and physiological characteristics. Both the original and the AMC-adapted E. coil cells could coexist with the native AMC microflora for one year or longer. When introduced into AMCs together with native pseudomonads, the AMC-adapted E. coil Z905-33 (pPHL7) cells were more competitive than the nonadapted cells.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Barkhatov Y.V., Khromechek E.B., Gubanov V.G.
Заглавие : Effects of the bacterial component on the viability of the Paramecium bursaria symbiotic complex (infusorian-zoochlorella)
Колич.характеристики :5 с
Место публикации : Russ. J. Ecol.: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 32, Is. 4. - P261-265. - ISSN 1067-4136, DOI 10.1023/A:1011314622045
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): paramecium bursaria--endosymbiosis--matter exchange cycle
Аннотация: A hypothesis is considered that the establishment and maintenance of mass exchange processes in the Paramecium bursaria symbiotic complex (infusorian-alga) depends on the presence of the third component-bacteria-whose role is underestimated but essential for completing the exchange cycle. The role of this component in the symbiotic cycle of Paramecium bursaria is possible to reveal using special bactericidal preparations specifically inhibiting the bacteria involved in the metabolic cycle but having no effect on the functions of the host cell and the zoochlorella population. Experiments with various preparations of this type were performed, and the response to treatment was estimated from growth rates of the symbiotic complex and its components. This allowed identification of an antibiotic (rifampicin) that had no adverse influence on free-living zoochlorella and chlorella-free paramecia (the basic components of the symbiotic cycle) but drastically inhibited the growth of the P. bursaria symbiotic complex, which could be interpreted as evidence in favor of the hypothesis concerning the existence of the third symbiotic component.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Popova L.Y.
Заглавие : Salt-dependent inhibition of light emitting of the luminescent microorganism Escherichia coli Z9051
Колич.характеристики :5 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2001. - Vol. 46, Is. 2. - P251-255. - ISSN 0006-3029
Примечания : Cited References: 12
Ключевые слова (''Своб.индексиров.''): bacterial luminescence--recombinant plasmid--salt concentration
Аннотация: The influence of some mineral salts on the recombinant strain Escherichia coli Z9051 was investigated. It was shown that the composition (NaCl, Na2SO4, MgCl2 and MgSO4) and concentration (5 and 10%) of the salts substantially affect the expression of genes for the luminescence system of fight-emitting bacteria cloned in the plasmid under the control of the lac-promoter. In some cases, the luminescence level of the microorganism in the presence of salts was similar to the luminescence level under catabolite repression by glucose, the more strong influence of the salts exceeding the effect of catabolite repression. The possibility of adaptation of the genetically modified microorganism to the salinity factor is discussed.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petrushkov V.N., Gibson B.G., Visser AJWG, Lee J...
Заглавие : Purification and ligand exchange protocols for antenna proteins from bioluminescent bacteria
Колич.характеристики :17 с
Место публикации : Methods Enzymol.: ACADEMIC PRESS INC, 2000. - Vol. 305. - P164-180. - ISSN 0076-6879
Примечания : Cited References: 18
Предметные рубрики: YELLOW FLUORESCENT PROTEIN
FISCHERI STRAIN Y-1
AMINO-ACID-SEQUENCE
VIBRIO-FISCHERI
PHOTOBACTERIUM-LEIOGNATHI
RIBOFLAVIN PROTEIN
LUMINOUS BACTERIUM
LUMAZINE PROTEIN
FMN
Y1
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr' A.P., Mogil'naya O.A., Tirranen L.S.
Заглавие : Architectonics of Flavobacterium sp. 56 and Flavobacterium sp. 22 colonies as exposed by transmission electron microscopy
Колич.характеристики :8 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1998. - Vol. 67, Is. 5. - P555-562. - ISSN 0026-2617
Примечания : Cited References: 21
Предметные рубрики: BACTERIAL
Ключевые слова (''Своб.индексиров.''): electron microscopy--colony architectonics--flavobacterium
Аннотация: Colonies of Flavobacterium sp. 56 and Flavobacterium sp. 22 were studied by electron microscopy without being washed off from solid nutrient medium and were shown to differ in the spatial arrangement and ultrastructure of their cells. Colonies of Flavobacterium sp. 56 contained a single type of cells, while those of Flavobacterium sp. 22 consisted of cells of two types differing in their ultrastructure. Within the colony volume, bacteria were oriented in a regular pattern characteristic of each strain studied. The evidence obtained relating to the ultrastructure, spatial orientation, and location of cells is sufficient for the unambiguous identification of bacterial strains and provides new information on colony architectonics.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gurevich Y.L., Teremova M.I., Latyntseva A.V.
Заглавие : Flocculation of bacterial cells in a culture with protozoans
Колич.характеристики :5 с
Место публикации : Izv. Akad. Nauk Ser. Biol.: MEZHDUNARODNAYA KNIGA, 1998. - Is. 3. - P351-355. - ISSN 0002-3329
Примечания : Cited References: 13
Аннотация: The initial phase of formation of the biogenic suspension was studied in experimental communities of bacteria and protozoans that simulate degradation of the phenol technogenic flows. Protozoans were shown to initiate formation of bacterial aggregates and increase the size of bacterial flocules several hundred times. Factors were found by the methods of mathematical planning, which markedly affect the size and amount of flocules. Unlike natural water bodies, in the sample communites, the presence of abiogenic substrate was not essential for aggregation of the bacteria, The aggregated bacterial cells make an important contribution to degradation of organic compounds.
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15.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A., Pisman T.I.
Заглавие : Catalase activity as a potential indicator of the reducer component of small closed ecosystems
Колич.характеристики :4 с
Место публикации : LIFE SCIENCES: LIFE SUPPORT SYSTEMS STUDIES-I. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1997. - Vol. 20: F4.6, F4.8, F4.2 and F4.9 Symposia of COSPAR Scientific Commission F on Life Sciences - Life Support System Studies-I, at the 31st COSPAR Scientific Assembly (JUL 14-SEP 21, 1996, BIRMINGHAM, ENGLAND), Is. 10. - P1945-1948. - ISBN 0273-1177, DOI 10.1016/S0273-1177(97)00630-3. - ISBN 0-08-043307-3
Примечания : Cited References: 8
Аннотация: Dynamics of catalase activity has been shown to reflect the growth curve of microorganisms in batch cultivation (celluloselythic bacteria Bacillus acidocaldarius and bacteria of the associated microflora Chlorella vulgaris). Gas and substrate closure of the three component ecosystems with spatially separated components "producer-consumer-reducer" (Chl, vulgaris-Paramecium caudatum-B. acidocaldarius, two bacterial strains isolated from the associated microflora Chl. vulgaris) demonstrated that the functioning of the reducer component can be estimated by the catalase activity of microorganisms of this component. (C) 1997 COSPAR. Published by Elsevier Science Ltd.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tyulkova N.A., Sandalova T.P.
Заглавие : Comparative study of temperature effects on bacterial luciferases
Колич.характеристики :10 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1996. - Vol. 61, Is. 2. - P205-214. - ISSN 0006-2979
Примечания : Cited References: 23
Предметные рубрики: BIOLUMINESCENCE
Ключевые слова (''Своб.индексиров.''): bacterial luciferase--temperature--activation energy
Аннотация: Effects of temperature on bioluminescent patterns of luciferases from luminescent bacteria Vibrio harveyi, Vibrio fischeri, Photobacterium leiognathi, and Photobacterium phosphoreum were studied. The highest luminescence level was observed at 15-25 degrees C for the luciferase from P. phosphoreum, at 20-30 degrees C for the V. fischeri and P. leiognathi enzymes, and at 30-37 degrees C for the enzyme from V. harveyi. All the luciferases were significantly stabilized at increased salt concentrations, at low pH values, or in the presence of dithiothreitol (DTT) and EDTA. The addition of DTT and EDTA affected the reversible stage of enzyme inactivation, while salts reduced the rate of the irreversible stage. A peak corresponding to aggregated protein was detected by gel chromatography of irreversibly inactivated luciferase. Activation energies were calculated for each luciferase in bioluminescent reactions with decanal, dodecanal, tetradecanal, and without aldehydes. The activation energy of the reaction with tetradecanal was much lower than those with the other aldehydes. The temperature dependence of the lifetime of the long-lived reaction intermediate showed that in the 10-30 degrees C interval all the luciferases, except for the enzyme from V. harveyi, have only one active form.
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17.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Kuznetsov A.M., Rodicheva E.K., Medvedeva S.E.
Заглавие : Biotesting of sewage and river water by liofilyzed luminous bacteria biotest
Колич.характеристики :5 с
Место публикации : FIELD SCREENING EUROPE: SPRINGER, 1997. - 1st International Conference on Field Screening Europe - Strategies and Techniques for On-Site Investigation and Monitoring of Contaminated Soil, Water and Air (SEP 29-OCT 01, 1997, KARLSRUHE, GERMANY). - P211-215. - ISBN 0-7923-4782-X
Примечания : Cited References: 0
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr A.P., Mogil'naya O.A., Pavlyuchenko E.L.
Заглавие : Architectonics of a mixed colony Flavobacterium sp 56+Bacillus cereus 60
Колич.характеристики :7 с
Место публикации : Izv. Akad. Nauk Ser. Biol.: MEZHDUNARODNAYA KNIGA, 1999. - Is. 3. - P378-384. - ISSN 0002-3329
Примечания : Cited References: 15
Аннотация: Electron microscopy has shown that the colonies obtained as a result of joint growth of bacterial monocultures Flavobacterium sp. 56 and Bacillus cereus 60 consist of three main cell types. Two cell types of the mixed colonies preserve the fine structure specific for bacteria in the monocultures. At the same time, formation of a large amount of the third type of cells is stimulated, which occur as single cells in the monoculture Flavobacterium sp. 56. The cell types are not intermixed in the colony volume and have certain spatial positions.
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19.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Bartsev S.I., Okhonin V.A.
Заглавие : Self-restoration of biocomponents as a mean to enhance biological life support systems reliability
Колич.характеристики :4 с
Место публикации : LIFE SCIENCES: ARTIFICIAL ECOSYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1999. - Vol. 24: F4 3 and F4 4 Symposia of COSPAR Scientific Commission F Held at 31st COSPAR Scientific Assembly (JUL 14-21, 1996, BIRMINGHAM, ENGLAND), Is. 3. - P393-396. - ISBN 0273-1177, DOI 10.1016/S0273-1177(99)00489-5
Примечания : Cited References: 5
Аннотация: One of the key problems of long-term space missions is limited service life of units. The only exceptions are biological components of biological Life Support Systems - higher plants or microorganisms. These components are capable of self-restoration: after complete disintegration, they can appear again from seeds or spores. The estimate of failure intensity of BLSS regeneration component includes: a number of self-sustained sections of the regeneration component; permissible boost (how many times can productivity of a component be increased); time required to repair (restore) a component; the crew existence time, when all LSS regeneration components fail; failure rate of one section of a regeneration component. Evaluations show that for hydrogen-oxidizing bacteria and micro-algae very high reliability is achieved even for one or two sections. In the case of higher plants (due to low rate of self-restoration) bio-regenerative module has to be divided into 10 self-sustained sections operating simultaneously. These measures can decrease the probability of catastrophe by a factor of 10 (C) 1999 COSPAR. Published by Elsevier Science Ltd.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V.A., Kudinova I.Y.
Заглавие : Practical enzymology course based on bioluminescence
Колич.характеристики :4 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 1999. - Vol. 14: 10th International Symposium on Bioluminescence and Chemiluminescence (1998, BOLOGNA, ITALY), Is. 4. - P189-192. - ISSN 1522-7235, DOI 10.1002/(SICI)1522-7243(199907/08)14:4189::AID-BIO5273.0.CO;2-E
Примечания : Cited References: 7
Ключевые слова (''Своб.индексиров.''): enzyme--science education--luciferase--bioluminescence
Аннотация: We describe our experience with laboratory courses in enzymology based on the phenomenon of bioluminescence. The soluble and immobilized enzymes of luminous bacteria are used and the practical enzymological course consists of four main courses: (1) training in measuring the activities of soluble and immobilized enzymes; (2) the investigation of kinetic characteristics (kinetic constants) and enzyme-substrate and enzyme-inhibitor interactions in the bacterial bioluminescent reaction; (3) The testing of physico-chemical characteristics of enzymes (pH, temperature, ion strength, etc.); (4) the effect of inhibitors on enzymes. Training is possible in groups of about ten persons. Our practice work has been introduced in the biological, pedagogical and physical departments of Krasnoyarsk State University. Students of the pedagogical department have created a popular and interesting series of laboratory works for high school children aged 14-17 years. Copyright (C) 1999 John Wiley & Sons, Ltd.
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