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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Терсков И.А., Гительзон И.И., Сидько Ф.Я., Окладников Ю.Н., Трубачев И.Н., Федорова Я.В., Волова Т.Г., Попов Н.И.
Заглавие : О биологической и кормовой ценности биомассы водородных бактерий : научное издание
Место публикации : Прикл. биохимия и микробиол. - 1984. - Т. 20, N 4. - С. 540-550. - ISSN 0555-1099
ГРНТИ : 34.27.51
Предметные рубрики: БИОМАССА
БИОХИМИЧЕСКИЙ СОСТАВ
КОРМОВАЯ ЦЕННОСТЬ
ВОДОРОДНЫЕ БАКТЕРИИ
ALCALIGENES EUTROPHUS
BIOMASS PRODUCTION
BIOLOGICAL VALUE
NUTRITIONAL VALUE
HYDROGEN BACTERIA
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Вид документа : Статья из сборника (однотомник)
Шифр издания : Е071/Б 63
Автор(ы) : Медведева С. Е.
Заглавие : Коллекция культур ибсо как база для исследований биолюминесценции й и грибов в ИБФ СО РАН
Параллельн. заглавия :Culture collection ibso as a basis for research of bioluminescence of bacteria and fungi in IBP SB RAS
Коллективы : Российская академия наук, Институт биофизики
Место публикации : Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона/ И. И. Гительзон, Т. Г. Волова, А. Г. Дегерменджи [и др.] ; ред., авт. предисл. Т. Г. Волова. - Новосибирск: Издательство Сибирского отделения Российской академии наук, 2019. - С. 24-39. - ISBN 978-5-7692-1650-3 (Шифр Е071/Б 63-478048446)
Примечания : Библиогр.: с. 37-39
УДК : 577 + 574 + 61
ББК : Е071я43 + Р252.0я43
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Петушков В.Н., Родионова Н.С., Белобров П.И.
Заглавие : Изучение эффективности работы биферментной системы NADH: FMN-оксидоредуктаза-люцифераза светящихся бактерий : научное издание
Место публикации : Биохимия. - 1985. - Т. 50, N 3. - С. 401-405. - ISSN 0320-9725
ГРНТИ : 31.27.17
Предметные рубрики: НАДН:ФМН-ОКСИДОРЕДУКТАЗА-ЛЮЦИФЕРАЗА
МЕХАНИЗМ РАБОТЫ
БАКТЕРИИ
СВЕТЯЩИЕСЯ
NADH:FMN-OXIDOREDUCTASE-LUCIFERASE
LUMINESCENT BACTERIA
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Белобров П.И., Воеводин В.А., Ерохин В.В., Львов Ю.М., Петушков В.Н., Пузырь А.П., Родионова Н.С.
Заглавие : Взаимодействие бактериальной люцефаразы с амфифильными молекулами в растворе, на поверхности воды и в пленках Ленгмюра-Блоджетт : научное издание
Коллективы :
Место публикации : Препр. - 1988. - N 92Б. - С. 1-28
ГРНТИ : 34.17.09
Предметные рубрики: БИОЛЮМИНЕСЦЕНЦИЯ
ЛЮЦИФЕРАЗА
СВОЙСТВА
БИОСЕНСОРЫ
BACTERIA LUCIFERASE
Р ОРЕ Т Е III SOLUTION WATER FILMS
В О Е О
Аннотация: Исследованы амфифильные свойства бакт. люциферазы в различных условиях и ее взаимодействие с длинноцепоч. алифатич. альдегидом и соответствующей жирной к-той. Методом малоуглового рассеяния рентген. лучей определены радиусы инерции фермента в р-ре и найдены зависимости их от состояния люциферазы. Исследована реология монослоев, полученных из белка, субстрата, продукта и их комбинаций со строго определенными соотношениями на поверхности различных субфаз. Соответствующие 'пи'-А изотермы показывают, что во всех случаях образуются твердые двумерные пленки, сжимаемость к-рых сильно зависит от соотношения компонент. Монослои перенесены на твердую подложку и получены мультислойные пленки на разных носителях. Рентгеновское исследование позволило определить ближний порядок в толстых пленках, к-рый также зависит от соотношения компонент. Метод позволяет конструировать регулярные неоднородности распределения люциферазы в монослое. Результаты электр. микроскопии чистых и смешанных ионо- и бислоев, перенесенных на твердые подложки, свидетельствуют в пользу микроэмульсионной модели амфифильности люциферазы. Получены ленгмюровские пленки с сохранением активности. Это открывает путь к новому поколению люциферазных твердотельных биосенсоров. Библ. 21
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Вид документа : Однотомное издание
Шифр издания : Е071/Б 63
Автор(ы) : Гительзон, Иосиф Исаевич, Волова, Татьяна Григорьевна, Дегерменджи, Андрей Георгиевич, Дегерменджи Н. Н., Шевырногов, Анатолий Петрович, Кратасюк В. А., Барцев, Сергей иванович, Болсуновский, Александр Яковлевич, Бондарь, Владимир Антонович, Буров А. Е., Величко В. В., Гладышев, Михаил Иванович, Есимбекова Е. Н., Дементьев Д. В., Есимбекова Е. Н., Задереев, Егор Сергеевич, Зотина Т. А., Косиненко, Сергей Васильевич, Медведева С. Е., Петушков В. Н., Печуркин, Николай Савельевич, Прокопкин И. Г., Пузырь А. П., Пуртов К. В., Рогозин, Денис Юрьевич, Родионова Н. С., Ронжин Н. О., Сомова, Лидия Александровна, Тихомиров, Александр Аполлинариевич, Тихомирова, Наталья Александровна, Трифонов С. В., Ушакова, Софья Аврумовна, Франк Л. А., Хромечек Е. Б., Шишацкая Е. И., Шуваев А. Н.
Заглавие : Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона
Выходные данные : Новосибирск: Издательство Сибирского отделения Российской академии наук, 2019
Колич.характеристики :292, [2] с.: ил., цв. ил.; 25 см.
Коллективы : Российская академия наук. Сибирское отделение, Институт биофизики (Красноярск)
Примечания : Рез. ст. англ. - Библиогр. в конце ст.
ISBN, Цена 978-5-7692-1650-3: 1635.00 р.
ГРНТИ : 31.27 + 76.03
УДК : 577 + 574 + 61
ББК : Е071я43 + Р252.0я43
Предметные рубрики: Экологическая биофизика
Медицинская биофизика
Ключевые слова (''Своб.индексиров.''): биолюминесценция--люцифераза--целентаразин--олигохеты--замкнутые экосистемы--управляемый биосинтез--наноалмазы--радиоизотопные методы--биосфера--жирные кислоты--системы жизнеобеспечения--меромиктические озера--геобиосфера--эволюция--глобальный климат--медицинская биофизика
Содержание : Краткий очерк истории, состояния и перспектив/ И. И. Гительзон. Коллекция культур ибсо как база для исследований биолюминесценции й и грибов в ИБФ СО РАН/ С. Е. Медведева. Биолюминесценция Мирового океана/ И. И. Гительзон, Л. А. Левин, А. С. Артемкин, Р. Н., Чепилов В. В., Молвинских С.Л., Черепанов О. А., Чугунов Ю. В., Караев Н. Д., Загородний Ю. А., Шевырногов А. П. Утюшев Р. Н. Бактериальная люцифераза в биолюминесцентном анализе/ В. А. Кратасюк, Е. Н. Есимбекова. Целентеразин-зависимые биолюминесцентные системы/ Л. А. Франк. Изучение химического механизма биолюминесценции грибов/ К. В. Пуртов, В. Н. Петушков, Н. С. Родионова. Исследование биолюминесценции сибирских почвенных олигохет/ Н. С. Родионова, А. А. Петушков. Экспериментальные модели замкнутых экосистем с расчетной долей человека как перспективное направление исследований по созданию биолого-технической системы жизнеобеспечения/ А. А. Тихомиров, С. А. Ушакова, Н. А. Тихомирова, С. В., Величко В. В. Трифонов С. В. Управляемый биосинтез: от параметрически управляемых продуцирующих биосистем до новейших биофизических технологий/ Т. Г. Волова, Е. И. Шишацкая. Биомедицинские приложения наноалмазов взрывного синтеза/ В. С. Бондарь, А. П. Пузырь, Н. О. Ронжин, А. В., Буров А. Е. Барон А. В. Применение радиоизотопных методов в институте биофизики СО РАН: от клеток крови до экосистем/ А. Я. Болсуновский, С. В. Косиненко, Т. А. Зотина, Д. В. Дементьев. Биосфера - взгляд сверху (экспрессные методы мониторинга биосферы в ИБФ СО РАН – ХХ–ХХI вв.)/ А. П. Шевырногов. Жирные кислоты в экологической биофизике водных систем/ М. И. Гладышев. Сравнительное исследование устойчивости стратификации и структуры трофической сети в меромиктических озерах Шира и Шунет (Южная Сибирь, Россия)/ Д. Ю. Рогозин, Е. С. Задереев, И. Г. Прокопкин [и др.]. Непрерывный рост интенсивности энерго-вещественных взаимодействий в эволюции геобиосферы Земли/ Н. С. Печуркин, А. Н. Шуваев, Л. А. Сомова. Малоразмерные модели биосферы и феноменология изменения глобального климата/ С. И. Барцев, А. Г. Дегерменджи. Направления развития биофизики в Красноярске/ А. Г. Дегерменджи.
Аннотация: Сборник посвящен широкому кругу исследований в области экологической биофизики – научного направления на стыке наук – от исследований на молекулярном уровне до вопросов управления большими природными экосистемами. Рассмотрены исторические вехи развития экологического направления биофизики. Основной акцент сборника основан на современных, актуальных достижениях красноярских биофизиков, которым удалось сохранить и развить многоплановые направления, которые были заложены в 50-х гг. ХХ века И. И. Гительзоном. Наряду с обзорными материалами и результатами фундаментальных исследований представлен ряд готовых к внедрению биотехнологий. Книга адресована биофизикам, экологам и химикам, а также преподавателям и студентам биофизических, биологических и экологических кафедр университетов.
Экземпляры :ИБФ-КФ(1)
Свободны : ИБФ-КФ(1)
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Neubauer, Darshan, Kolmakova, Olesya, Woodhouse, Jason, Taube, Robert, Mangelsdorf, Kai, Gladyshev, Michail, Premke, Katrin, Grossart, Hans-Peter
Заглавие : Zooplankton carcasses stimulate microbial turnover of allochthonous particulate organic matter
Колич.характеристики :16 с
Коллективы : Projekt DEAL
Место публикации : ISME J.: SPRINGERNATURE, 2021. - Article in press. - ISSN 1751-7362, DOI 10.1038/s41396-020-00883-w. - ISSN 1751-7370(eISSN)
Примечания : Cited References:83. - Open Access funding enabled and organized by Projekt DEAL.
Предметные рубрики: FRESH-WATER
SEASONAL-CHANGES
CARBON
LAKE
DECOMPOSITION
DEGRADATION
Аннотация: Carbon turnover in aquatic environments is dependent on biochemical properties of organic matter (OM) and its degradability by the surrounding microbial community. Non-additive interactive effects represent a mechanism where the degradation of biochemically persistent OM is stimulated by the provision of bioavailable OM to the degrading microbial community. Whilst this is well established in terrestrial systems, whether it occurs in aquatic ecosystems remains subject to debate. We hypothesised that OM from zooplankton carcasses can stimulate the degradation of biochemically persistent leaf material, and that this effect is influenced by the daphnia:leaf OM ratio and the complexity of the degrading microbial community. Fresh Daphnia magna carcasses and C-13-labelled maize leaves (Zea mays) were incubated at different ratios (1:1, 1:3 and 1:5) alongside either a complex microbial community (50 mu m) or solely bacteria (0.8 mu m). C-13 stable-isotope measurements of CO2 analyses were combined with phospholipid fatty acids (PLFA) analysis and DNA sequencing to link metabolic activities, biomass and taxonomic composition of the microbial community. Our experiments indicated a significantly higher respiration of leaf-derived C when daphnia-derived OM was most abundant (i.e. daphnia:leaf OM ratio of 1:1). This process was stronger in a complex microbial community, including eukaryotic microorganisms, than a solely bacterial community. We concluded that non-additive interactive effects were a function of increased C-N chemodiversity and microbial complexity, with the highest net respiration to be expected when chemodiversity is high and the degrading community complex. This study indicates that identifying the interactions and processes of OM degradation is one important key for a deeper understanding of aquatic and thus global carbon cycle.
WOS
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Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Gros J.-B., Lasseur C., Tikhomirov A.A., Manukovsky N.S., Ushakova S.A., Zolotukhin I.G., Tirranen L.S., Borodina E.V., Kovalev V.S.
Заглавие : Wheat growth on neutral and soil-like substrates: Carbon dioxide exchange and microflora
Место публикации : Acta Horticulturae. - 2004. - Vol. 644. - С. 243-248
Ключевые слова (''Своб.индексиров.''): carbon dioxide--closed ecological system
Аннотация: The soil-like substrate (SLS), which is a potential candidate for employment in closed ecological systems, has been tested. CO2 exchange, composition and numbers of soil microflora have been examined in .wheat-SLS. system. The results produced have been compared to analogous characteristics of .wheat-neutral substrate. system. A hydroponic method was used under wheat growing on the neutral substrate (expanded clay aggregate). Plants for both studies were grown in closed environment from seed to physiological maturity. In the .wheat-SLS. system, the net photosynthetic rate of canopy was positive in the course of 6.55 days after planting. The net photosynthetic rate of canopy in .wheat-neutral substrate. system was positive in the entire course of vegetation. According to calculations in the course of vegetation, photosynthesis has withdrawn 3.28 kg m-2 CO2 from the .wheat-SLS. system and 3.40 kg m-2 CO2 from the .wheat-neutral substrate. system. On the SLS dominant among bacteria were the spore-forming bacteria from Bacillus genus, among fungi . from Trichoderma genus. In the hydroponic cultivation on neutral substrate dominant were bacteria from Pseudomonas genus, most commonly found fungi were species from Fusarium and Botrytis genera.
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Degermendzhy A.G., Zadereev E.S., Rogozin D.Y., Prokopkin I.G., Barkhatov Y.V., Tolomeev A.P., Khromechek E.B., Janse J.H., Mooij W.M., Gulati R.D.
Заглавие : Vertical stratification of physical, chemical and biological components in two saline lakes Shira and Shunet (South Siberia, Russia)
Место публикации : Aquatic Ecology. - 2010. - Vol. 44, Is. 3. - С. 619-632. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-010-9336-6
Ключевые слова (''Своб.индексиров.''): ciliates--cryptomonas--fishless lakes--gammarus--mathematical modelling--meromictic lakes--phytoplankton--stratification--sulphur bacteria--amphipod--bacterium--biomass--ciliate--ecosystem modeling--flagellate--meromictic lake--microbial community--numerical model--physicochemical property--phytoplankton--population density--saline lake--salinity--stratification--thermocline--trophic interaction--vertical profile--zooplankton--khakassia--lake shira--lake shunet--russian federation--siberia--amphipoda--bacteria (microorganisms)--ciliophora--copepoda--cryptomonas--cryptomonas sp.--gammaridae--gammarus--gammarus lacustris--phytomastigophorea--protista--rotifera
Аннотация: A feature of meromictic lakes is that several physicochemical and biological gradients affect the vertical distribution of different organisms. The vertical stratification of physical, chemical and biological components in saline, fishless meromictic lakes Shira and Shunet (Siberia, Russia) is quite different mainly because both mean depth and maximum depth of lakes differ as well as their salinity levels differ. The chemocline of the Lake Shira, as in many meromictic lakes, is inhabited by bacterial community consisting of purple sulphur and heterotrophic bacteria. As the depth of the chemocline is variable, the bacterial community does not attain high densities. The mixolimnion in Lake Shira, which is thermally stratified in summer, also creates different habitat for various species. The distribution of phytoplankton is non-uniform with its biomass peak in the metalimnion. The distribution of zooplankton is also heterogeneous with rotifers and juvenile copepods inhabiting the warmer epilimnion and older copepods found in the cold but oxic hypolimnion. The amphipod Gammarus lacustris which can be assigned to the higher trophic link in the fishless lake's ecosystem, such as Lake Shira, is also distributed non-uniformly, with its peak density generally observed in the thermocline region. The chemocline in Lake Shunet is located at the depth of 5 m, and unlike in Lake Shira, due to a sharp salinity gradient between the mixolimnion and monimolimnion, this depth is very stable. The mixolimnion in Lake Shunet is relatively shallow and the chemocline is inhabited by (1) an extremely dense bacterial community; (2) a population of Cryptomonas sp.; and (3) ciliate community comprising several species. As the mixolimnion of Lake Shunet is not thermally stratified for long period, the phytoplankton and zooplankton populations are not vertically stratified. The gammarids, however, tend to concentrate in a narrow layer located 1-2 m above the chemocline. We believe that in addition to vertical inhomogeneities of both physicochemical parameters, biological and physical factors also play a role in maintaining these inhomogeneities. We conclude that the stratified distributions of the major food web components will have several implications for ecosystem structure and dynamics. Trophic interactions as well as mass and energy flows can be significantly impacted by such heterogeneous distributions. Species spatially separated even by relatively short distances, say a few centimetres will not directly compete. Importantly, we demonstrate that not only bacteria, phytoflagellates and ciliate tend to concentrate in thin layers but also larger-sized species such Gammarus (amphipods) can also under certain environmental conditions have stratified distribution with maxima in relatively thin layer. As the vertical structure of the lake ecosystem is rather complex in such stratified lakes as ours, the strategy of research, including sampling techniques, should consider potentially variable and non-homogeneous distributions. В© 2010 The Author(s).
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Kudenko Y.A., Ushakova S.A., Tirranen L.S., Gribovskaya I.A., Gros J.-B., Lasseur C.
Заглавие : Use of human wastes oxidized to different degrees in cultivation of higher plants on the soil-like substrate intended for closed ecosystems
Место публикации : Advances in Space Research. - 2010. - Vol. 46, Is. 6. - С. 744-750. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2010.02.024
Ключевые слова (''Своб.индексиров.''): life support systems--microflora--mineralized human wastes--phototrophic unit--wet incineration--alternating electromagnetic field--bioregenerative life support systems--degree of oxidations--denitrifying microorganisms--growth and development--higher plants--human waste--life support systems--mass exchange--microbiotas--microflora--microscopic fungi--mineralized human wastes--nutrient solution--oxidation level--phytopathogenic bacteria--plant productivity--soil-like substrate--wheat plants--biomolecules--electromagnetic fields--irrigation--magnetic field effects--metabolism--metabolites--oxidation--plants (botany)--soils--solvent extraction--wastes--waste incineration
Аннотация: To close mass exchange loops in bioregenerative life support systems more efficiently, researchers of the Institute of Biophysics SB RAS (Krasnoyarsk, Russia) have developed a procedure of wet combustion of human wastes and inedible parts of plants using H2O2 in alternating electromagnetic field. Human wastes pretreated in this way can be used as nutrient solutions to grow plants in the phototrophic unit of the LSS. The purpose of this study was to explore the possibilities of using human wastes oxidized to different degrees to grow plants cultivated on the soil-like substrate (SLS). The treated human wastes were analyzed to test their sterility. Then we investigated the effects produced by human wastes oxidized to different degrees on growth and development of wheat plants and on the composition of microflora in the SLS. The irrigation solution contained water, substances extracted from the substrate, and certain amounts of the mineralized human wastes. The experiments showed that the human wastes oxidized using reduced amounts of 30% H2O2: 1 ml/g of feces and 0.25 ml/ml of urine were still sterile. The experiments with wheat plants grown on the SLS and irrigated by the solution containing treated human wastes in the amount simulating 1/6 of the daily diet of a human showed that the degree of oxidation of human wastes did not significantly affect plant productivity. On the other hand, the composition of the microbiota of irrigation solutions was affected by the oxidation level of the added metabolites. In the solutions supplemented with partially oxidized metabolites yeast-like microscopic fungi were 20 times more abundant than in the solutions containing fully oxidized metabolites. Moreover, in the solutions containing incompletely oxidized human wastes the amounts of phytopathogenic bacteria and denitrifying microorganisms were larger. Thus, insufficiently oxidized sterile human wastes added to the irrigation solutions significantly affect the composition of the microbiological component of these solutions, which can ultimately unbalance the system as a whole. В© 2010 COSPAR. Published by Elsevier Ltd. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Degermendzhy A.G., Gulati R.D.
Заглавие : Understanding the mechanisms of blooming of phytoplankton in Lake Shira, a saline lake in Siberia (the Republic of Khakasia)
Место публикации : Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - С. 331-340. - ISSN 13862588 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon budget--cyanobacteria--heterotrophic bacteria--hydrogen sulphide--mathematical models of stratification--meromictic lakes--microbial loop--stratification--trophic scheme--algal bloom--ecosystem modeling--limiting factor--nutrient availability--phytoplankton--saline lake--trophic interaction--russian federation--algae--bacteria (microorganisms)--cyanobacteria--lyngbya--lyngbya contorta
Аннотация: The paper summarises the results of a three-year research study (European Union Grant: INTAS 97-0519) aimed at investigating the planktonic populations and trophic organization of the Lake Shira ecosystem - a saline lake in Khakasia, Siberia. The lake exhibits a stable summer-autumn stratification of the chemical-biological components. The mechanisms responsible for the 'blooming' of phytoplankton in the deeper layers were investigated in greater detail, using data from both field and laboratory experiments. The spectra of nutrition were examined to estimate the relationships between the specific growth rates of the hydrobionts and the influence of the limiting factors: light, nutrients. The observed heterotrophic capability of a metalimnetic phytoplankton population might help explain the development in the deeper waters of Lyngbya contorta. The scheme of trophic interactions was put up, based on the assessment of the carbon pools and carbon flows in the pelagic zone of the lake. A mathematical model of the vertical structure of the lake's plankton populations was constructed, using the ecosystem description and data of vertical turbulent diffusion. The role of light and nutrient limitations and grazing mortality in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with the field observations, e.g. for the different patterns of the peaks, and for the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. Based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been theoretically discriminated for the first time. It has also been ascertained that irrespective of the depth the allochthonous substances limiting bacterial growth, the bacteria are uniformly distributed over depth and can serve as an indicator of the presence of limitation (the effect of autostabilisation in space). Of indisputable interest to limnology are the specific methods developed for understanding the functioning of Lake Shira ecosystem. These include the autostabilisation of the limiting factors, the on-the-spot fluorescent method of determining the three classes of microalgae, the algal mixotrophy and the planktonic population interactions and feedbacks, and development of a more sensitive, bioluminescent method for mapping the nonhomogeneities. Owing to a balanced combination of classical approaches (field observations, in situ data on production-decomposition) and the more recent ones (satellite monitoring, biophysical methods of estimating interactions of populations, mathematical models based on the field and experimental data), many of the structural-function relationships in the ecosystem can now be explained, and the models can provide 'mutual control and mutual agreement' between the data collected using different approaches.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., van Stokkum IHM, Gobets B..., van Mourik F..., Lee J..., van Grondelle R..., Visser AJWG
Заглавие : Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria
Колич.характеристики :6 с
Место публикации : J. Phys. Chem. B: AMER CHEMICAL SOC, 2003. - Vol. 107, Is. 39. - P10934-10939. - ISSN 1520-6106, DOI 10.1021/jp034266e
Примечания : Cited References: 52
Предметные рубрики: TIME-RESOLVED FLUORESCENCE
VIBRIO-FISCHERI Y1
FEMTOSECOND SOLVATION DYNAMICS
FLAVIN ADENINE-DINUCLEOTIDE
PHOTOBACTERIUM-LEIOGNATHI
BIOLOGICAL WATER
SOLVENT DYNAMICS
DIELECTRIC-RELAXATION
MOLECULAR-DYNAMICS
TRYPTOPHAN
Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by similar to7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Van Stokkum I.H.M., Gobets B., Van Mourik F., Lee J., Van Grondelle R., Visser A.J.W.G.
Заглавие : Ultrafast fluorescence relaxation spectroscopy of 6,7-dimethyl-(8-ribityl)-lumazine and riboflavin, free and bound to antenna proteins from bioluminescent bacteria
Место публикации : Journal of Physical Chemistry B. - 2003. - Vol. 107, Is. 39. - С. 10934-10939. - ISSN 15206106 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacteria--bioluminescence--chemical relaxation--chromophores--dielectric properties--proteins--solvents--bioluminescent bacteria--dimethyl ribityl lumazine--photobacterium leiognathi--riboflavin--ultrafast fluorescence relaxation spectroscopy--fluorescence
Аннотация: The solvation dynamics of interesting bioluminescent chromophores have been determined, using subpicosecond and wavelength-resolved fluorescence spectroscopy, in combination with global analysis of the multidimensional data sets. The systems investigated comprise the free ligands 6,7-dimethyl-(8-ribityl)-lumazine (lumazine) and riboflavin in an aqueous buffer and both ligands when noncovalently bound to two bacterial bioluminescent antenna proteins: lumazine protein (from Photobacterium leiognathi) and the blue fluorescent protein (from Vibrio fischeri Y1). Fluorescence spectral relaxation of the free ligands is complete within a few picoseconds. Subsequently, the fluorescence intensity increases by ?7% on a time scale of 15-30 ps. Fluorescence spectral relaxation of the protein-bound ligands is largely complete within 1 ps but reveals a small red shift with a minor, but distinctly longer, relaxation time than that of the free ligands, which is tentatively assigned to the relaxation of protein-bound water in the vicinity of the excited chromophore.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Brilkov A.V., Loginov I.A., Morozova E.V., Pechurkin N.S.
Заглавие : Trends in microevolution of microbial populations in open systems
Место публикации : Doklady Biochemistry and Biophysics. - 2005. - Vol. 404, Is. 1-6. - С. 349-352. - ISSN 16076729 (ISSN) , DOI 10.1007/s10628-005-0111-x
Ключевые слова (''Своб.индексиров.''): article--bacterial phenomena and functions--bacterium--biological model--culture technique--escherichia coli--evolution--genetics--growth, development and aging--mathematics--methodology--mutation--nanotechnology--ph--physiology--population dynamics--time--bacteria--bacterial physiology--cell culture techniques--escherichia coli--evolution--hydrogen-ion concentration--mathematics--models, biological--mutation--nanotechnology--population dynamics--time factors
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Medvedeva S.E.
Заглавие : Transfer of xenobiotics through cell membranes of luminous bacteria
Место публикации : Luminescence. - 1999. - Vol. 14, Is. 5. - С. 267-270. - ISSN 15227235 (ISSN)
Ключевые слова (''Своб.индексиров.''): luminous bacteria--toxicant--ultrastructure--bacterial dna--edetic acid--toluene--xenobiotic agent--article--cell membrane--dna damage--drug effect--luminescence--metabolism--photobacterium--sensitivity and specificity--transport at the cellular level--ultrastructure--vibrio--biological transport--cell membrane--dna damage--dna, bacterial--edetic acid--luminescence--photobacterium--sensitivity and specificity--toluene--vibrio--xenobiotics
Аннотация: The influence of some chemical substances on luminous bacteria was studied to elucidate the interrelation between the xenobiotics action on bacterial luminescence and cell ultrastructure. Such substances as quinones, phenols, chlorides of heavy metals (in concentrations of substances inhibiting luminescence by 50%) resulted in damaging effects upon bacteria: a lot of cells had damage of membranes due to changes in their permeability. It was found that the high concentration of EDTA and toluene decreased the luminescence and caused the condensation of DNA-fibrils and the cell damage after long-term and short-term action. The low concentration of EDTA and toluene did not decrease the bacterial luminescence; the noticeable damage of cell membranes did not take place during short-term treatment. However, the long action of these substances changed the membrane permeability resulting in increased sensitivity of bacterial luminescence to some toxic substances. Copyright В© 1999 John Wiley & Sons, Ltd.
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15.

16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rogozin D.Yu., Pimenov N.V., Kosolapov D.B., Chan'kovskaya Yu.V., Degermendzhy A.G.
Заглавие : Thin-layer vertical distribution of purple sulfur bacteria in the chemoclines of meromictic lakes Shira and Shunet (Khakasia, Southern Siberia)
Место публикации : Doklady Akademii Nauk. - 2005. - Vol. 400, Is. 3. - С. 426-429. - ISSN 08695652 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacteria--bathymetry--biochemistry--geochemistry--physical chemistry--water bacteriology--bathometers--vertical distribution--lakes
Аннотация: Vertical inhomogeneity of microbial population, biogeochemical processes and physicochemical characteristics of chemocline was revealed using specially created stratification bathometer (sampler) consisting of syringes horizontally placed one over another on a carrying frame.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kirensky L.V., Gitelson I.I., Terskov I.A., Kovrov B.G., Lisovsky G.M., Okladnikov Y.N.
Заглавие : Theoretical and experimental decisions in the creation of an artificial ecosystem for human life support in space.
Место публикации : Life sciences and space research. - 1971. - Vol. 9. - С. 75-80. - ISSN 00759422 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--bacterium--biotechnology--chlorella--energy metabolism--human--instrumentation--man machine interaction--metabolism--microclimate--space flight--weightlessness--bacteria--biotechnology--chlorella--ecological systems, closed--energy metabolism--environment, controlled--humans--life support systems--man-machine systems--space flight--weightlessness
Аннотация: All of man's former space flights were not real ventures into space in the biological sense, as his life was supported with unregenerated earth supplies. The coming stage of space exploration requires man's long existence in the cosmos and on the other planets. This stage of man's activity outside the earth become possible only by creating small man-made ecosystems, permitting the support of his metabolism by the recycling of substances of the terrestrial biosphere. Creation of such systems is a new scientific and technical task. Man-made ecosystems are a new product of man's activity, which have no complete analogy, either in nature, or in technology. Stochastic mechanisms, which stabilize biogeocenosis, cannot be effective in small ecosystems. A technique of parametric control over biosynthesis made it possible to calculate, and put to practice, an ecosystem for man with a cyclic regeneration of the atmosphere, water and, partially, food. The specific bio-technological properties of small man-made ecosystems are being analysed. The possibility of their application for man's excursions into space and for the settlement of other planets is being considered.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J.
Заглавие : The yellow bioluminescence bacterium, Vibrio fischeri Y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence FMN protein
Место публикации : Biochemical and Biophysical Research Communications. - 1995. - Vol. 211, Is. 3. - С. 774-779. - ISSN 0006291X (ISSN) , DOI 10.1006/bbrc.1995.1880
Ключевые слова (''Своб.индексиров.''): riboflavin--article--bioluminescence--fluorescence--nonhuman--priority journal--protein analysis--protein synthesis--vibrio--vibrionaceae--bacterial proteins--chromatography, gel--chromatography, thin layer--flavin mononucleotide--flavoproteins--luminescence--riboflavin--spectrometry, fluorescence--support, u.s. gov't, p.h.s.--vibrio--bacteria (microorganisms)--photobacterium--vibrio--vibrio fischeri
Аннотация: The yellow bioluminescence Y1 strain of Vibrio fischeri can produce a 22 kDa protein with either FMN or riboflavin as a bound fluorophore. Both forms are active for shifting the bioluminescence spectral maximum. The fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. The bioluminescence activity of the riboflavin Y1 protein contrasts with the inactivity of the related Photobacterium type.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vinogradov M.E., Gitelzon I.I., Sorokin Yu.I.
Заглавие : The vertical structure of a pelagic community in the tropical ocean
Место публикации : Marine Biology. - 1970. - Vol. 6, Is. 3. - С. 187-194. - ISSN 00253162 (ISSN) , DOI 10.1007/BF00347226
Аннотация: The vertical structure of the ecosystem in the euphotic zone of the western Equatorial Pacific has been studied. The studies were based on a continuous sounding of the bioluminescence field, with simultaneous, vertically aimed sampling made with the aid of a 5l water bottle and plankton nets. The bioluminescence field has a two-maxima structure with a more pronounced and permanent lower maximum found in the oligotrophic regions as deep as 60 to 100 m. In the narrow (10 m) layer of maximum bioluminescence, the concentration of zooplankton, as well as the concentration, activity and production of bacteria and phytoplankton, are several times higher than those in immediately adjacent waters at greater or lesser depth. At the same time, the concentration of nutrient salts in that layer diminishes sharply and approaches zero in the overlying water. Analysis of the data obtained shows that the production processes above the lower maximum layer are dependent not only on the turbulent influx of nutrient salts from the underlying layers, but also on the horizontal supply from the zone of upwelling. A pelagic community, in its different time aspects from the moment of water ascending until its sinking in the convergence zone, is suggested as a common system for modelling. В© 1970 Springer-Verlag.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Makhutova O.N., Sushchik N.N., Kalacheva G.S.
Заглавие : The value of fatty acid composition of tricylglycerols and polar lipids of seston at analysis of food spectra of microzooplancton in small reservoir Buguch
Место публикации : Doklady Akademii Nauk. - 2004. - Vol. 395, Is. 4. - С. 562-565. - ISSN 08695652 (ISSN)
Ключевые слова (''Своб.индексиров.''): algae--bacteria--chromatographic analysis--chromatography--organic compounds--food spectra--microzooplancton--polar lipids--microorganisms
Аннотация: Food spectrum of microzooplancton in fresh-water ecological systems is lowly studied area. However, these organisms play very important role in transfer of substance and energy in water reservoirs. Traditional method of analysis of food in the microzooplancton does not makes it possible to determine real assimilation of organic substance. Therefore, biochemical methods have been developed which are based on analysis of compositions of fatty acids of organic substance. This leads to determining of the food assimilation. Fatty-acid compositions of lipids allow specialists to determine specific groups of organisms such as bacteria, microalgae etc. Data of the analysis are presented.
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