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A bioluminescent assay for detecting melanocortin-1 receptor (MC1R) gene polymorphisms R160W, R151C, and D294H / E. E. Bashmakova [et al.] // Mol. Biol. - 2015. - Vol. 49, Is. 6. - P852-857, DOI 10.1134/S0026893315050039 . - ISSN 0026-8933
Кл.слова (ненормированные):
bioluminescent assay -- MC1R receptor -- melanoma -- single nucleotide polymorphisms
Аннотация: Several polymorphisms in the melanocortin-1 receptor gene (MC1R) have been associated with melanoma risk. In particular, rs1805007, rs1805008, and rs1805009 mutations, which result in R151C, R160W, and D294H amino acid substitutions, respectively, and are associated with the phenotype of red-hair mutations, have also been connected with melanoma and nonmelanoma skin cancer risks. This work describes a method of detecting these polymorphisms using primer extension with subsequent dual bioluminescent assay. Model plasmids carrying polymorphic MC1R fragments, as well as several clinical DNA samples, were tested using the proposed technique. The results agreed well with those obtained by Sanger sequencing. © 2015, Pleiades Publishing, Inc.

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Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Blokhin Cancer Research Center, Moscow, Russian Federation
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russian Federation
Voino-Yasenetskii Krasnoyarsk State Medical University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Bashmakova, E. E.; Krasitskaya, V. V.; Bondar, A. A.; Kozlova, A. V.; Ruksha, T. G.; Frank, L. A.
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A heuristic neural network model in the research of properties of evolutionary trajectories / S. Bartsev, P. Baturina // INTERNATIONAL WORKSHOP ADVANCED TECHNOLOGIES IN MATERIAL SCIENCE, : IOP PUBLISHING LTD, 2019. - Vol. 537: International Workshop on Advanced Technologies in Material Science, (APR 04-06, 2019, Krasnoyarsk, RUSSIA). - Ст. 042001. - (IOP Conference Series-Materials Science and Engineering), DOI 10.1088/1757-899X/537/4/042001. - Cited References:21 . -

РУБ Engineering, Mechanical + Materials Science, Multidisciplinary
Рубрики:
SEQUENCE SPACE
Аннотация: There is considerable data on molecular evolution, but there remains no approach to systematizing them within the framework of the key problems of biology. To search for the most common properties of evolving systems, the heuristic method has been proposed. Artificial networks of formal neurons were chosen as the heuristic model object. The paper examines the divergent component of evolutionary trajectory formation. As a result of the simulation, the dependence of the potential variability parameter on the position of the fitness function landscape was obtained. The simulation results are in agreement with the real data of molecular evolution experiments.

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Держатели документа:
RAS, SB, Inst Biophys, Fed Res Ctr,Krasnoyarsk Sci Ctr, 50 Akad Gorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, 79 Svobodny Pr, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Bartsev, S.; Baturina, P.

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A heuristic neural network model in the research of properties of evolutionary trajectories / S. Bartsev, P. Baturina // IOP Conference Series: Materials Science and Engineering : Institute of Physics Publishing, 2019. - Vol. 537: International Workshop on Advanced Technologies in Material Science, Mechanical and Automation Engineering - MIP: Engineering-2019 (4 April 2019 through 6 April 2019, ) Conference code: 149243, Is. 4, DOI 10.1088/1757-899X/537/4/042001
Кл.слова (ненормированные):
Molecular biology -- Artificial networks -- Common property -- Evolving systems -- Fitness functions -- Heuristic model -- Molecular evolution -- Neural network model -- Trajectory formation -- Heuristic methods
Аннотация: There is considerable data on molecular evolution, but there remains no approach to systematizing them within the framework of the key problems of biology. To search for the most common properties of evolving systems, the heuristic method has been proposed. Artificial networks of formal neurons were chosen as the heuristic model object. The paper examines the divergent component of evolutionary trajectory formation. As a result of the simulation, the dependence of the potential variability parameter on the position of the fitness function landscape was obtained. The simulation results are in agreement with the real data of molecular evolution experiments. © Published under licence by IOP Publishing Ltd.

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center, Krasnoyarsk Scientific Center SB RAS, 50, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, 79 Svobodny pr., Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Bartsev, S.; Baturina, P.

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A low-cost underwater particle tracking velocimetry system for measuring in situ particle flux and sedimentation rate in low-turbulence environments / S. Simoncelli [et al.] // Limnol. Oceanogr. Methods. - 2019, DOI 10.1002/lom3.10341 . - Article in press. - ISSN 1541-5856
Аннотация: We describe a low-cost three-dimensional underwater particle tracking velocimetry system to directly measure particle settling rate and flux in low-turbulence aquatic environments. The system consists of two waterproof cameras that acquire stereoscopic videos of sinking particles at 48 frames s?1 over a tunable sampling volume of about 45 ? 25 ? 24 cm. A dedicated software package has been developed to allow evaluation of particle velocities, concentration and flux, but also of morphometric parameters such as particle area, sinking angle, shape irregularity, and density. Our method offers several advantages over traditional approaches, like sediment trap or expensive in situ camera systems: (1) it does not require beforehand particle collection and handling; (2) it is not subjected to sediment trap biases from turbulence, horizontal advection, or presence of swimmers, that may alter particulate load and flux; (3) the camera system enables faster data processing and flux computation at higher spatial resolution; (4) apart from the particle settling rates, the particle size distribution, and morphology is determined. We tested the camera system in Lake Stechlin (Germany) in low turbulence and mean flow, and analyzed the morphological properties and settling rates of particles to determine their sinking behavior. The particle flux assessed from conventional sediment trap measurements agreed well with that determined by our system. By this, the low-cost approach demonstrated its reliability in low turbulence environments and a strong potential to provide new insights into particulate carbon transport in aquatic systems. Extension of the method to more turbulent and advective conditions is also discussed. © 2019 The Authors. Limnology and Oceanography: Methods published by Wiley Periodicals, Inc. on behalf of Association for the Sciences of Limnology and Oceanography.

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Держатели документа:
Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Berlin, Germany
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Potsdam University, Institute for Biochemistry and Biology, Potsdam, Germany

Доп.точки доступа:
Simoncelli, S.; Kirillin, G.; Tolomeev, A. P.; Grossart, H. -P.

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A New Bias Site for Epigenetic Modifications: How Non-Canonical GC Base Pairs Favor Mechanochemical Cleavage of DNA / D. A. Semyonov, I. V. Eltsov, Y. D. Nechipurenko // Bioessays. - 2020. - Vol.: 42, Issue : 11. - Ст. 2000051, DOI 10.1002/bies.202000051. - Cited References:55. - The authors thank Dr. Dmitry M. Graifer, Prof. Vladimir A. Reznikov, and Dr. Anastasia Anashkina, for useful discussion. The authors thank the Prof. D. R. Forsdyke whose advice on improving the style of the manuscript was very useful. This work was supported by the program of the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology and the Program of Fundamental Research for State Academies for years 2013-2020, project no. 01201363818. The work was supported by the Russian Ministry of Science and High Education (grant #05.604.21.0234, unique number RFMEFI60419X0234) . - ISSN 0265-9247. - ISSN 1521-1878

РУБ Biochemistry & Molecular Biology + Biology
Рубрики:
NUCLEIC-ACIDS
   CYTOSINE
   MUTAGENESIS
   N-4-METHOXYCYTOSINE
Кл.слова (ненормированные):
5-methylcytosines -- mechanochemical cleavage of DNAs -- molecular basis of -- epigenetics -- tautomers -- transient base pairs in DNAs -- wobble base pairs
Аннотация: Properties of non-canonical GC base pairs and their relations with mechanochemical cleavage of DNA are analyzed. A hypothesis of the involvement of the transient GC wobble base pairs both in the mechanisms of the mechanochemical cleavage of DNA and epigenetic mechanisms involving of 5-methylcytosine, is proposed. The hypothesis explains the increase in the frequency of the breaks of the sugar-phosphate backbone of DNA after cytosines, the asymmetric character of these breaks, and an increase in break frequency in CpG after cytosine methylation. As an alternative hypothesis, probable implication of GC(+)Hoogsteen base pairs is considered, which now exemplify the best-studied non-canonical GC base pairs in the DNA double helix. Also see the video abstract here https://youtu.be/EUunVWL0ptw.

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Файл:  доступ к статье - 0
https://onlinelibrary.wiley.com/doi/epdf/10.1002/bies.202000051
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Akademgorodok 50, Krasnoyarsk 660036, Russia.
Novosibirsk State Univ, Novosibirsk, Russia.
Russian Acad Sci, Engelhardt Inst Mol Biol, Moscow, Russia.

Доп.точки доступа:
Semyonov, Denis A.; Eltsov, Ilia, V; Nechipurenko, Yury D.; program of the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology; Program of Fundamental Research for State Academies for years 2013-2020 [01201363818]; Russian Ministry of Science and High Education [05.604.21.0234, RFMEFI60419X0234]

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A study of forest vegetation dynamics in the south of the Krasnoyarskii Krai in spring / M. Chernetskiy [et al.] // Advances in Space Research. - 2011. - Vol. 48, Is. 5. - P819-825, DOI 10.1016/j.asr.2011.04.032 . - ISSN 0273-1177
Кл.слова (ненормированные):
EVI -- Forestry -- MODIS -- NDVI -- Remote sensing -- Vegetation phenology -- Accurate measurement -- Annual time series -- Carbon exchange -- Data series -- Dynamic state -- Enhanced vegetation index -- EVI -- Forest vegetation -- Global scale -- Growth dynamics -- Interannual variability -- Moderate resolution imaging spectroradiometer -- MODIS -- NDVI -- Normalized difference vegetation index -- Principal components analysis -- Remote sensing applications -- Remote sensing data -- Satellite data -- Spatial structure -- Spring season -- Terrestrial ecosystems -- Vegetation dynamics -- Vegetation phenology -- Biology -- Climate models -- Dynamics -- Ecosystems -- Estimation -- Forestry -- Monitoring -- Principal component analysis -- Radiometers -- Remote sensing -- Satellite imagery -- Timber -- Time series -- User interfaces -- Vegetation -- Carbon -- Ecosystems -- Forests -- Image Analysis -- Plants -- Remote Sensing -- Time Series Analysis
Аннотация: Remote sensing applications have greatly enhanced ability to monitor and manage in the areas of forestry. Accurate measurements of regional and global scale vegetation dynamics (phenology) are required to improve models and understanding of inter-annual variability in terrestrial ecosystem carbon exchange and climate-biosphere interactions. Study of vegetation phenology is required for understanding of variability in ecosystem. In this paper, monitoring of vegetation dynamics using time series of satellite data is presented. Vegetation variability (vegetation rate) in different topoclimatic areas is investigated. Original software using IDL interactive language for processing of satellite long-term data series was developed. To investigate growth dynamics vegetation rate inferred from remote sensing was used. All estimations based on annual time series of Moderate Resolution Imaging Spectroradiometer (MODIS) imagery. Vegetation rate for Enhanced Vegetation Index (EVI) and Normalized Difference Vegetation Index (NDVI) was calculated using MODIS data. The time series covers spring seasons of each of 9 years, from 2000 to 2008. Comparison of EVI and NDVI derived growth rates has shown that NDVI derived rates reveal spatial structure better. Using long-term data of vegetation rates variance was estimated that helps to reveal areas with anomalous growth rate. Such estimation shows sensitivity degree of different areas to different topoclimatic conditions. Woods of heights depend on spatial topoclimatic variability unlike woods of lowlands. Principal components analysis shows vegetation with different rate conditions. Also it reveals vegetation of same type in areas with different conditions. It was demonstrated that using of methods for estimating the dynamic state of vegetation based on remote sensing data enables successful monitoring of vegetation phenology. В© 2011 COSPAR. Published by Elsevier Ltd. All rights reserved.

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Держатели документа:
Institute of Biophysics of SB RAS, Akademgorodok 50/50, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Chernetskiy, M.; Pasko, I.; Shevyrnogov, A.; Slyusar, N.; Khodyayev, A.

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A study of the properties and efficacy of microparticles based on P(3HB) and P(3HB/3HV) loaded with herbicides / R. Vijayamma, H. J. Maria, S. Thomas [et al.] // J. Appl. Polym. Sci. - 2021, DOI 10.1002/app.51756 . - Article in press. - ISSN 0021-8995
Кл.слова (ненормированные):
biodegradable -- drug delivery systems -- microparticles -- Agricultural robots -- Controlled drug delivery -- Solubility -- Targeted drug delivery -- Weed control -- 3-Hydroxybutyrate -- Average diameter -- Biodegradable -- Drug-delivery systems -- Human impact -- Metribuzin -- Micro particles -- Poly-3-hydroxybutyrate -- Polyhydroxyalkanoates -- Property -- Herbicides
Аннотация: The wide use of pesticides in agriculture has caused uncontrolled distribution of these chemicals in the environment, calling for the development and investigation of new environmentally friendly formulations, which would reduce human impact on nature. In the present study, the metribuzin (MET), tribenuron-methyl (TBM), and fenoxaprop-P-ethyl (FPE) herbicides were encapsulated in microparticles of degradable microbial polymers – polyhydroxyalkanoates (PHAs) – of two types – poly-3-hydroxybutyrate [P(3HB)] and poly(3-hydroxybutyrate-co-3-hydroxyvalerate [P(3HB/3HV)]. The use of P(3HB) resulted in higher yields of microparticles (63% to 79%) and larger sizes of the particles, whose average diameter was 0.60 ± 0.06–0.75 ± 0.11 ?m, while the average diameter of copolymer particles varied between 0.43 ± 0.12 and 0.55 ± 0.05 ?m. Encapsulation efficiency was rather determined by the type of herbicide and its solubility, varying from 24.7% to 48.2%. In vitro herbicide release from microparticles to water was affected by herbicide solubility and PHA chemical composition. The readily soluble MET showed the highest release rate, and over 30 days, 64% and 78% of the encapsulated amounts were released from P(3HB) and P(3HB/3HV) microparticles, respectively. High herbicidal activity of microparticles loaded with metribuzin and tribenuron-methyl was demonstrated in the laboratory stands of the Elsholtzia ciliata weed plant. © 2021 Wiley Periodicals LLC.

Scopus
Держатели документа:
Institute of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
International and Inter University Centre for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, India
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Krasnoyarsk Regional Center of Research Equipment of Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
L.V. Kirensky Institute of Physics, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Scientific Laboratory, Reshetnev Siberian State University of Science and Technology, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Vijayamma, R.; Maria, H. J.; Thomas, S.; Shishatskaya, E. I.; Kiselev, E. G.; Nemtsev, I. V.; Sukhanova, A. A.; Volova, T. G.

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A test system for tick-borne encephalitis virus detection based on bioluminescent immunoassay / A. N. Kudryavtsev, L. P. Burakova, K. A. Barinova, L. A. Frank // J. Sib. Fed. Univ. - Biol. - 2020. - Vol. 13, Is. 3. - С. 310-321, DOI 10.17516/1997-1389-0296 . - ISSN 1997-1389
Кл.слова (ненормированные):
Bioluminescent microassay -- Hybrid protein 14D5a-Rm7 -- Tick-borne encephalitis virus (TBEV)
Аннотация: The tick-borne encephalitis virus (TBEV) is the causative agent of one of the most severe human neuroinfections. The infection transmitted by ixodid ticks is spread throughout the forest and forest-steppe zones of the temperate climatic belt of the Eurasian continent, including the Siberian region of the Russian Federation. Despite the availability of commercial analytical systems for the detection of TBEV, the task of developing approaches to a quick and reliable analysis that can be performed routinely, particularly in environmental studies, remains topical. A solid-phase bioluminescent immunoassay for determining the tick-borne encephalitis virus (TBEV) in ticks was developed. The assay is based on the hybrid protein consisting of a modified thermostable version of Renilla muelleri luciferase and a single-chain mini-antibody to protein E. This unique protein had been obtained and investigated by the authors earlier. The current study describes the expression of the hybrid protein in two different strains of recombinant E. coli cells. The optimal conditions for obtaining a highly purified protein were found. The bioluminescent reaction of the luciferase domain was triggered with the help of the stable natural form of the substrate, a Ca-dependent coelenterazine-binding protein, the recombinant variant of which was obtained by the authors. The conditions for production and storage of the immunoassay components (the hybrid protein, the stable form of the luciferase substrate, and activated microplates) were determined. Using the developed test system, more than 900 tick samples were analyzed for TBEV. In terms of sensitivity (89.5%) and specificity (98.9%), the proposed method is not inferior to colorimetric detection and is much simpler and faster than the latter. © Siberian Federal University. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kudryavtsev, A. N.; Burakova, L. P.; Barinova, K. A.; Frank, L. A.

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All Ca2+-binding loops of light-sensitive ctenophore photoprotein berovin bind magnesium ions: The spatial structure of Mg2 +-loaded apo-berovin / L. P. Burakova [et al.] // J. Photochem. Photobiol. B Biol. - 2016. - Vol. 154. - P57-66, DOI 10.1016/j.jphotobiol.2015.11.012 . - ISSN 1011-1344
Кл.слова (ненормированные):
Aequorin -- Bioluminescence -- Calcium -- Coelenterazine -- Obelin
Аннотация: Light-sensitive photoprotein berovin accounts for a bright bioluminescence of ctenophore Beroe abyssicola. Berovin is functionally identical to the well-studied Ca2+-regulated photoproteins of jellyfish, however in contrast to those it is extremely sensitive to the visible light. Berovin contains three EF-hand Ca2+-binding sites and consequently belongs to a large family of the EF-hand Ca2+-binding proteins. Here we report the spatial structure of apo-berovin with bound Mg2+ determined at 1.75 A. The magnesium ion is found in each functional EF-hand loop of a photoprotein and coordinated by oxygen atoms donated by the side-chain groups of aspartate, carbonyl groups of the peptide backbone, or hydroxyl group of serine with characteristic oxygen-Mg2+ distances. As oxygen supplied by the side-chain of the twelfth residue of all Ca2+-binding loops participates in the magnesium ion coordination, it was suggested that Ca2+-binding loops of berovin belong to the mixed Ca2+/Mg2+ rather than Ca2+-specific type. In addition, we report an effect of physiological concentration of Mg2+ on bioluminescence of berovin (sensitivity to Ca2+, rapid-mixed kinetics, light-sensitivity, thermostability, and apo-berovin conversion into active protein). The different impact of physiological concentration of Mg2+ on berovin bioluminescence as compared to hydromedusan photoproteins was attributed to different affinities of the Ca2 +-binding sites of these photoproteins to Mg2+. © 2015 Elsevier B.V. All rights reserved.

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Держатели документа:
Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, China
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Akademgorodok 50, Krasnoyarsk, Russian Federation
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, 15 Datun Road, Beijing, China
IHuman Institute, ShanghaiTech University, 99 Haike Road, Shanghai, China

Доп.точки доступа:
Burakova, L. P.; Natashin, P. V.; Malikova, N. P.; Niu, F.; Pu, M.; Vysotski, E. S.; Liu, Z.-J.
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10.

Amino acid composition of epilithic biofilm and benthic animals in a large Siberian river / A. A. Kolmakova [et al.] // Freshwater Biology. - 2013. - Vol. 58, Is. 10. - P2180-2195, DOI 10.1111/fwb.12200 . - ISSN 0046-5070
Кл.слова (ненормированные):
Amino acids -- Epilithic microalgae and cyanobacteria -- Nutritive quality -- River ecosystem -- Zoobenthos
Аннотация: We studied amino acid (AA) composition of epilithic biofilms and zoobenthos near the shore at a middle section of the Yenisei River (Siberia, Russia). We hypothesised that there was an imbalance between the composition and content of amino acids in the biofilm and its consumers, the zoobenthos, as well as between those in the zoobenthos and fish. Based on monthly sampling from 2007 to 2010, there was seasonal variation in AA profiles in the epilithic biofilms, probably caused by the succession of microalgal and cyanobacterial species. Overall, there was an imbalance in the percentage of the essential amino acids (lysine and histidine) between benthic animals and their food (the epilithic biofilm), which suggests that benthic animals may be limited by food quality. Moreover, the zoobenthos had a significantly higher content of AA, relative to carbon, than the biofilm. Based on sampling in 2012, there was an imbalance between the AA profiles of zoobenthos and that of their main consumer, the Siberian grayling (Thymallus arcticus), particularly in the percentages of two essential amino acids, lysine and leucine. In terms of overall content of essential amino acids, the nutritional value to fish of gammarids, which have recently invaded the river, was significantly lower than that of indigenous taxa, trichopteran and chironomid larvae. В© 2013 John Wiley & Sons Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Science, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kolmakova, A.A.; Gladyshev, M.I.; Kalachova, G.S.; Kravchuk, E.S.; Ivanova, E.A.; Sushchik, N.N.

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11.

Amino Acid Composition of Green Microalgae and Diatoms, Cyanobacteria, and Zooplankton (Review) / A. A. Kolmakova, V. I. Kolmakov // Inland Water Biol. - 2019. - Vol. 12, Is. 4. - P452-461, DOI 10.1134/S1995082919040060 . - ISSN 1995-0829
Кл.слова (ненормированные):
amino acids -- aquatic ecosystem -- Cyanobacteria -- microalgae -- zooplankton -- Bacillariophyta -- Cyanobacteria
Аннотация: Abstract: We have reviewed foreign and domestic literature devoted to the study of the amino acid (AA) composition of aquatic organisms representing major groups of producers (green microalgae and diatoms, and cyanobacteria) and primary consumers (zooplankton). Based on published data, we estimate the composition of essential and nonessential AAs of microalgae, cyanobacteria, and zooplankton and determine their differences. It is concluded that the AA composition of major groups of plankton is heterogeneous. The role of AAs as a limiting factor for the development of herbivorous zooplankton is discussed. The prospects and the need for further study of AA composition in order to develop a complete theory of functioning of aquatic ecosystems have been demonstrated. © 2019, Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Kolmakova, A. A.; Kolmakov, V. I.

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12.

Amino Acid Composition of Green Microalgae and Diatoms, Cyanobacteria, and Zooplankton (Review) / A. A. Kolmakova, V. I. Kolmakov // Inland Water Biol. - 2019. - Vol. 12, Is. 4. - P452-461, DOI 10.1134/S1995082919040060. - Cited References:72. - State task of the basic research program of the Russian Federation, topic number VI.51.S. . - ISSN 1995-0829. - ISSN 1995-0837

РУБ Marine & Freshwater Biology
Рубрики:
BIOCHEMICAL-COMPOSITION
PROTEIN-CONTENT
CRUSTACEAN ZOOPLANKTON
Кл.слова (ненормированные):
amino acids -- microalgae -- Cyanobacteria -- zooplankton -- aquatic ecosystem
Аннотация: We have reviewed foreign and domestic literature devoted to the study of the amino acid (AA) composition of aquatic organisms representing major groups of producers (green microalgae and diatoms, and cyanobacteria) and primary consumers (zooplankton). Based on published data, we estimate the composition of essential and nonessential AAs of microalgae, cyanobacteria, and zooplankton and determine their differences. It is concluded that the AA composition of major groups of plankton is heterogeneous. The role of AAs as a limiting factor for the development of herbivorous zooplankton is discussed. The prospects and the need for further study of AA composition in order to develop a complete theory of functioning of aquatic ecosystems have been demonstrated.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Kolmakova, A. A.; Kolmakov, V., I; State task of the basic research program of the Russian Federation [VI.51.S]

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13.

Amino acids in prospective feeds for fish aquaculture: A review of experimental data / V. I. Kolmakov, A. A. Kolmakova // J. Sib. Fed. Univ. - Biol. - 2020. - Vol. 13, Is. 4. - С. 424-442, DOI 10.17516/1997-1389-0332 . - ISSN 1997-1389
Кл.слова (ненормированные):
Amino acids -- Aquaculture -- Diet -- Fish feed -- Fishmeal
Аннотация: Total dependence of aquaculture on imports of feeds can significantly compromise Russia's food supply security; hence the task of designing new domestic feeds is of primary importance. A major challenge of world aquaculture is providing fish with a balanced diet, in particular, with a well-balanced composition of amino acids. In this review, we analyze international publications on the composition of amino acids in fish feed over the last two years. We summarize the data on the amino acid composition of prospective food sources for fish aquaculture: agricultural crops, insects, annelids, higher crustaceans, animal waste, phytoplankton, zooplankton, macrophytes, and microorganisms. We performed a comparative analysis of the composition of amino acids in promising aquafeed sources and fish flour traditionally used for fish feeding. Based on the results, we conclude that each of the examined food sources can be used as a component of fish nutrition and offers realistic prospects for production of alternative feeds. To ensure high quality of fish aquaculture products, the priority should be given to development of technologies which allow to control amino acid feed composition during the production process with respect to fish species-specific needs. It is hoped that this review will be useful to researchers and practitioners involved in designing and producing new domestic aquafeed for fish. © Siberian Federal University. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kolmakov, V. I.; Kolmakova, A. A.

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14.

An alternative approach to solar system exploration providing safety of human mission to Mars [Text] / J. I. Gitelson [et al.] ; ed.: G Horneck, ME Vazquez, Vazquez, ME // SPACE LIFE SCIENCES: MISSIONS TO MARS, RADIATION BIOLOGY, AND PLANTS AS A FOUNDATION FOR LONG-TERM LIFE SUPPORT SYSTEMS IN SPACE. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: F0 1 and F1 3-F2 3 Symposia of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 1. - P. 17-24, DOI 10.1016/S0273-1177(02)00657-9. - Cited References: 8 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
SPACE
Аннотация: For systematic human Mars exploration, meeting crew safety requirements, it seems perspective to assemble into a spacecraft: an electrical rocket, a well-shielded long-term life support system, and a manipulator-robots operating in combined "presence effect" and "master-slave" mode. The electrical spacecraft would carry humans to the orbit of Mars, providing short distance (and low signal time delay) between operator and robot-manipulators, which are landed on the surface of the planet. Long-term hybrid biological and physical/chemical LSS could provide environment supporting human health and well being. Robot-manipulators operating in "presence effect" and "master-slave" mode exclude necessity of human landing on Martian surface decreasing the level of risk for crew. Since crewmen would not have direct contact with the Martian environment then the problem of mutual biological protection is essentially reduced. Lightweight robot-manipulators, without heavy life support systems and without the necessity of returning to the mother vessel, could be sent as scouts to different places on the planet surface, scanning the most interesting for exobiological research site. Some approximate estimations of electric spacecraft, long-term hybrid LSS, radiation protection and mission parameters are conducted and discussed. (C) 2002 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, J.I.; Bartsev, S.I.; Mezhevikin, V.V.; Okhonin, V.A.; Horneck, G \ed.\; Vazquez, ME \ed.\

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15.

An elementary multistage discrete model of soil organic matter transformations with a continuous scale of stability / S. I. Bartsev, A. A. Pochekutov // Ecol. Model. - 2019. - Vol. 393. - P61-65, DOI 10.1016/j.ecolmodel.2018.12.012 . - ISSN 0304-3800
Кл.слова (ненормированные):
Kinetics of soil organic matter transformations -- Model of soil organic matter transformations -- Soil organic matter -- Biogeochemistry -- Biological materials -- Decay (organic) -- Organic compounds -- Soils -- Continuous scale -- Discrete modeling -- Elementary model -- Law of mass action -- Multistage process -- Realistic model -- Soil organic matters -- Transformation process -- Mathematical transformations -- biotransformation -- chemical alteration -- decomposition -- numerical model -- reaction kinetics -- soil organic matter
Аннотация: The proposed elementary mathematical model of formation and decomposition of soil organic matter (SOM) is based on using equations of chemical kinetics to describe the multistage process of SOM transformation. The model both describes each step of transformation in accordance with the law of mass action and postulates the trend of increasing stability of the matter towards further transformation, which is common for all steps. Analysis of the model demonstrates that it is extremely difficult to construct a realistic model of SOM dynamics by assembling elementary models of the type presented in this study into the full description of SOM transformation processes. © 2018 Elsevier B.V.

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Institute of Fundamental Biology and Biotechnology of Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Bartsev, S. I.; Pochekutov, A. A.

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16.

Antifungal activity of P3HB microparticles containing tebuconazole / A. M. Shershneva [et al.] // J. Environ. Sci. Health Part B Pestic. Food Contamin. Agric. Wastes. - 2019, DOI 10.1080/03601234.2018.1550299 . - Article in press. - ISSN 0360-1234
Кл.слова (ненормированные):
Antifungal activity -- encapsulation -- microparticles -- poly-3-hydroxybutyrate -- tebuconazole -- Agricultural chemicals -- Emulsification -- Encapsulation -- Fungicides -- Anti-fungal activity -- Average diameter -- Encapsulation efficiency -- Fusarium moniliforme -- Micro-particles -- Phytopathogenic fungi -- Poly-3-hydroxybutyrate -- Tebuconazole -- Fungi
Аннотация: In this study, tebuconazole (TEB)-loaded poly-3-hydroxybutyrate (P3HB)-based microparticles were developed and comprehensively characterized. TEB-loaded microparticles with the initial loading amounts of the fungicide of 10, 25, and 50% of the polymer mass (TEB 10, TEB 25, and TEB 50%) were prepared using emulsion technique. Encapsulation efficiency of TEB varied from 59 to 86%. As the loading amount was increased, the average diameter of microparticles increased too, from 41.3 to 71.7 µm, while zeta potential was not influenced by TEB loading, varying between –32.6 and –35.7 mV. TEB was gradually released from the microparticles to the model medium, and after 60 d, from 25 to 43% of TEB was released depending on the content of the encapsulated fungicide. The data obtained from in vitro TEB release were fitted to different mathematical models. It was shown that the release profiles of TEB could be best explained by the Zero-order, Higuchi, and Hixson–Crowell models. The antifungal activity of the P3HB/TEB microparticles against phytopathogenic fungi Fusarium moniliforme and Fusarium solani was demonstrated by in vitro tests conducted in Petri dishes. Thus, hydrophobic agrochemicals (TEB) can be effectively encapsulated into P3HB microparticles to construct slow-release formulations. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.

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Держатели документа:
Siberian Federal University, Institute of Fundamental Biology and Biotechnology, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Shershneva, A. M.; Murueva, A. V.; Zhila, N. O.; Volova, T. G.

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17.

Associating particulate essential fatty acids of the ?3 family with phytoplankton species composition in a Siberian reservoir / N. N. Sushchik [et al.] // Freshwater Biology. - 2004. - Vol. 49, Is. 9. - P1206-1219, DOI 10.1111/j.1365-2427.2004.01263.x . - ISSN 0046-5070
Кл.слова (ненормированные):
Cyanobacteria -- Diatoms -- Fatty acids -- Food quality -- Species composition -- cyanobacterium -- diatom -- fatty acid -- food quality -- food web -- growing season -- phytoplankton -- reservoir -- Eurasia -- Siberia -- Anabaena -- Anabaena flos-aquae -- Aphanizomenon -- Aphanizomenon flos-aquae -- Bacillariophyta -- Cyanobacteria -- Cyclotella -- Dinophyceae -- Flos -- Microcystis -- Microcystis aeruginosa -- Planktothrix -- Planktothrix agardhii -- Stephanodiscus
Аннотация: 1. We studied variation in the composition of fatty acids in the seston of a small freshwater reservoir with changes in phytoplankton composition during four growth seasons. We focused on the dynamics of the ?3 fatty acids because of their potential importance for zooplankton nutrition. 2. Total diatoms were related to the 20:5?3 fatty acid (eicosapentaenoic, EPA) content in seston. Among two dominant diatom genera, Cyclotella was not associated with EPA content. In contrast, there was a significant correlation between Stephanodiscus and the percentage contribution and content of EPA throughout the study. Hence, freshwater diatoms can differ strongly in content of the essential EPA. 3. We considered abundant cyanobacteria as a potential source of 18:3?3 fatty acid (linolenic, ALA) to aquatic food webs. Among four dominant cyanobacteria species, two (Anabaena flos-aquae and Planktothrix agardhii) showed significant correlation with the ALA content of the seston, while the other two (Aphanizomenon flos-aquae and Microcystis aeruginosa) did not. 4. Dinophyta had a relatively high level of 22:6?3 (docosahexaenoic, DHA) for freshwater species and can be also a source of EPA to aquatic food webs. 5. Our results show that various species of diatoms as well as cyanobacteria can be of contrasting nutritional value for zooplankton because of their different content of the essential PUFAs. Diatoms, which are low in EPA, could not be considered as a valuable food, while some field populations of cyanobacteria might be valuable sources of essential ALA.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Krasnoyarsk State Agric. University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sushchik, N.N.; Gladyshev, M.I.; Makhutova, O.N.; Kalachova, G.S.; Kravchuk, E.S.; Ivanova, E.A.

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18.

Associating particulate essential fatty acids of the omega 3 family with phytoplankton species composition in a Siberian reservoir [Text] / N. N. Sushchik [et al.] // Freshw. Biol. - 2004. - Vol. 49, Is. 9. - P. 1206-1219, DOI 10.1111/j.1365-2427.2004.01263.x. - Cited References: 48 . - ISSN 0046-5070

РУБ Marine & Freshwater Biology
Рубрики:
ENCLOSED EXPERIMENTAL ECOSYSTEM
   FOOD QUALITY
   BENTHIC INVERTEBRATES
   GREEN-ALGAE
   CYANOBACTERIA
   DAPHNIA
   MICROALGAE
   ZOOPLANKTON
   LAKE
   APHANIZOMENON
Кл.слова (ненормированные):
cyanobacteria -- diatoms -- fatty acids -- food quality -- species composition
Аннотация: 1. We studied variation in the composition of fatty acids in the seston of a small freshwater reservoir with changes in phytoplankton composition during four growth seasons. We focused on the dynamics of the omega3 fatty acids because of their potential importance for zooplankton nutrition. 2. Total diatoms were related to the 20:5omega3 fatty acid (eicosapentaenoic, EPA) content in seston. Among two dominant diatom genera, Cyclotella was not associated with EPA content. In contrast, there was a significant correlation between Stephanodiscus and the percentage contribution and content of EPA throughout the study. Hence, freshwater diatoms can differ strongly in content of the essential EPA. 3. We considered abundant cyanobacteria as a potential source of 18:3omega3 fatty acid (linolenic, ALA) to aquatic food webs. Among four dominant cyanobacteria species, two (Anabaena flos-aquae and Planktothrix agardhii) showed significant correlation with the ALA content of the seston, while the other two (Aphanizomenon flos-aquae and Microcystis aeruginosa) did not. 4. Dinophyta had a relatively high level of 22:6omega3 (docosahexaenoic, DHA) for freshwater species and can be also a source of EPA to aquatic food webs. 5. Our results show that various species of diatoms as well as cyanobacteria can be of contrasting nutritional value for zooplankton because of their different content of the essential PUFAs. Diatoms, which are low in EPA, could not be considered as a valuable food, while some field populations of cyanobacteria might be valuable sources of essential ALA.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Krasnoyarsk State Agr Univ, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sushchik, N.N.; Gladyshev, M.I.; Makhutova, O.N.; Kalachova, G.S.; Kravchuk, E.S.; Ivanova, E.A.

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19.

Author Correction: Plants with genetically encoded autoluminescence (Nature Biotechnology, (2020), 10.1038/s41587-020-0500-9) / T. Mitiouchkina, A. S. Mishin, L. G. Somermeyer [et al.] // Nat. Biotechnol. - 2020, DOI 10.1038/s41587-020-0578-0 . - Article in press. - ISSN 1087-0156
Аннотация: In the version of this article initially published online, a sentence was missing from the Acknowledgements section: The authors would like to acknowledge the work of Genomics Core Facility of the Skolkovo Institute of Science and Technology, which performed the sequencing and bioinformatic analysis. The error has been corrected in the HTML and PDF versions of the article. © 2020, The Author(s), under exclusive licence to Springer Nature America, Inc.

Scopus
Держатели документа:
Planta LLC, Moscow, Russian Federation
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation
Institute of Science and Technology Austria, Klosterneuburg, Austria
Pirogov Russian National Research Medical University, Moscow, Russian Federation
Institute of Biophysics, Krasnoyarsk Science Center of the Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Aivok LLC, Zelenograd, Moscow, Russian Federation
Botanical Garden of Lomonosov Moscow State University, Moscow, Russian Federation
Synthetic Biology Group, MRC London Institute of Medical Sciences, London, United Kingdom
Institute of Clinical Sciences, Faculty of Medicine and Imperial College Centre for Synthetic Biology, Imperial College London, London, United Kingdom

Доп.точки доступа:
Mitiouchkina, T.; Mishin, A. S.; Somermeyer, L. G.; Markina, N. M.; Chepurnyh, T. V.; Guglya, E. B.; Karataeva, T. A.; Palkina, K. A.; Shakhova, E. S.; Fakhranurova, L. I.; Chekova, S. V.; Tsarkova, A. S.; Golubev, Y. V.; Negrebetsky, V. V.; Dolgushin, S. A.; Shalaev, P. V.; Shlykov, D.; Melnik, O. A.; Shipunova, V. O.; Deyev, S. M.; Bubyrev, A. I.; Pushin, A. S.; Choob, V. V.; Dolgov, S. V.; Kondrashov, F. A.; Yampolsky, I. V.; Sarkisyan, K. S.

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20.

Biodegradable polymers - Perspectives and applications in agriculture / E. G. Kiselev, N. O. Zhila, T. G. Volova // IOP Conference Series: Earth and Environmental Science : IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст. 012036, DOI 10.1088/1755-1315/689/1/012036
Кл.слова (ненормированные):
Biodegradable polymers -- Ecosystems -- Fungi -- Glycerol -- Monounsaturated fatty acids -- Oilseeds -- Pesticides -- Substrates -- Sunflower oil -- Fenoxaprop-p-ethyl -- Natural materials -- Pesticide formulations -- Poly-3-hydroxybutyrate -- Polyhydroxyalkanoates -- Productive process -- Strategy of constructions -- Various substrates -- Palm oil
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.

Scopus
Держатели документа:
School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics, SB, RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kiselev, E. G.; Zhila, N. O.; Volova, T. G.

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