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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Karpowicz M., Feniova I., Gladyshev M. I., Ejsmont-Karabin J., Gorniak A., Zielinski P., Dawidowicz P., Kolmakova A. A., Dzialowski A. R.
Заглавие : The stoichiometric ratios (C:N:P) in a pelagic food web under experimental conditions
Место публикации : Limnologica: Elsevier GmbH, 2019. - Vol. 77. - Ст.125690. - ISSN 00759511 (ISSN) , DOI 10.1016/j.limno.2019.125690
Аннотация: Interactions between phytoplankton and zooplankton affect the overall functioning of lakes. Herbivores are habitually confronted with food of inferior quality, usually a result of low nutrient concentrations in plant material. Large-bodied cladocerans are better competitors for food than small-bodied species but they are more vulnerable to low food quality. Understanding the effects of food quality on zooplankton structure and competition between small - large bodied herbivorous is of considerable interest. We want to find out how differences in C:N:P ratios between phytoplankton and zooplankton communities affect their abundances in a freshwater food web. We want also to assess the role of phytoplankton and zooplankton as sinks of the phosphorus and nitrogen. Therefore, we conducted a 31-day mesocosms experiment with water from a mesotrophic and a eutrophic lake (with natural plankton communities). To simulate changes in the plankton communities large-bodied Daphnia magna and Daphnia pulicaria were added. Samples for zooplankton, phytoplankton and water chemistry were taken every 10 days. Samples for elemental analysis (C:N:P) of seston and zooplankton were collected on the first, and on the final day of the experiment. Our mesocosms experiment showed mismatch in C:P between seston (high) and zooplankton (low), which suggests that most of the phosphorus is incorporated in zooplankton biomass. This evidenced that zooplankton is an effective sink of phosphorus, while nitrogen is accumulated mainly by primary producers. Our results also indicated more stability in stoichiometry with increasing trophic levels of organisms. However, there were significant changes in the zooplankton structure. The increasing dominance of large Daphnia resulted in reduction of C:P ratio in zooplankton. Low food quality (C:P) did not limit the growth of large Daphnia in the experimental conditions, which competed effectively with small planktonic cladocerans and with Rotifera. Over time, inedible algae began to dominate resulting in increase of relative biomass of periphyton grazers, which suggests that plankton community is transformed into littoral system in mesocosms for about 30 days. © 2019
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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Bartsev S., Baturina P.
Заглавие : A heuristic neural network model in the research of properties of evolutionary trajectories
Место публикации : IOP Conference Series: Materials Science and Engineering: Institute of Physics Publishing, 2019. - Vol. 537: International Workshop on Advanced Technologies in Material Science, Mechanical and Automation Engineering - MIP: Engineering-2019 (4 April 2019 through 6 April 2019, ) Conference code: 149243, Is. 4. - , DOI 10.1088/1757-899X/537/4/042001
Аннотация: There is considerable data on molecular evolution, but there remains no approach to systematizing them within the framework of the key problems of biology. To search for the most common properties of evolving systems, the heuristic method has been proposed. Artificial networks of formal neurons were chosen as the heuristic model object. The paper examines the divergent component of evolutionary trajectory formation. As a result of the simulation, the dependence of the potential variability parameter on the position of the fitness function landscape was obtained. The simulation results are in agreement with the real data of molecular evolution experiments. © Published under licence by IOP Publishing Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Inzhevatkin E. V., Baron A. V., Maksimov N. G., Volkova M. B., Puzyr A. P., Bondar V. S.
Заглавие : EPR Spectrometric Estimation of the Distribution of Intravenously Injected Nanodiamonds in Mice
Колич.характеристики :7 с
Коллективы : Russian Foundation for Basic Research [16-04-00999]
Место публикации : Biol. Bull: PLEIADES PUBLISHING INC, 2019. - Vol. 46, Is. 3. - С. 277-283. - ISSN 1062-3590, DOI 10.1134/S1062359019020079. - ISSN 1608-3059(eISSN)
Примечания : Cited References:56. - This work was supported by the Russian Foundation for Basic Research, project no. 16-04-00999.
Предметные рубрики: DRUG-DELIVERY
DETONATION NANODIAMONDS
NANOMATERIALS
PARTICLES
Аннотация: The distribution in mice of intravenously injected modified nanodiamonds (MNDs) obtained by detonation synthesis was studied using electron paramagnetic resonance (EPR) spectrometry. It has been shown that 2.5 h after MND injection into the tail vein of mice, the nanoparticles accumulate mainly in the lungs and liver of animals; much smaller amounts of nanoparticles were found in the kidneys and heart. The presence of MNDs in the samples of blood, spleen, brain, and thigh muscles of mice was not detected within the sensitivity of the method used.
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Вид документа : Статья из журнала
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Автор(ы) : Simoncelli S., Kirillin G., Tolomeev A. P., Grossart H. -P.
Заглавие : A low-cost underwater particle tracking velocimetry system for measuring in situ particle flux and sedimentation rate in low-turbulence environments
Место публикации : Limnol. Oceanogr. Methods: Wiley Blackwell, 2019. - Article in press. - ISSN 15415856 (ISSN) , DOI 10.1002/lom3.10341
Аннотация: We describe a low-cost three-dimensional underwater particle tracking velocimetry system to directly measure particle settling rate and flux in low-turbulence aquatic environments. The system consists of two waterproof cameras that acquire stereoscopic videos of sinking particles at 48 frames s?1 over a tunable sampling volume of about 45 ? 25 ? 24 cm. A dedicated software package has been developed to allow evaluation of particle velocities, concentration and flux, but also of morphometric parameters such as particle area, sinking angle, shape irregularity, and density. Our method offers several advantages over traditional approaches, like sediment trap or expensive in situ camera systems: (1) it does not require beforehand particle collection and handling; (2) it is not subjected to sediment trap biases from turbulence, horizontal advection, or presence of swimmers, that may alter particulate load and flux; (3) the camera system enables faster data processing and flux computation at higher spatial resolution; (4) apart from the particle settling rates, the particle size distribution, and morphology is determined. We tested the camera system in Lake Stechlin (Germany) in low turbulence and mean flow, and analyzed the morphological properties and settling rates of particles to determine their sinking behavior. The particle flux assessed from conventional sediment trap measurements agreed well with that determined by our system. By this, the low-cost approach demonstrated its reliability in low turbulence environments and a strong potential to provide new insights into particulate carbon transport in aquatic systems. Extension of the method to more turbulent and advective conditions is also discussed. © 2019 The Authors. Limnology and Oceanography: Methods published by Wiley Periodicals, Inc. on behalf of Association for the Sciences of Limnology and Oceanography.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feniova I., Sakharova E., Karpowicz M., Gladyshev M. I., Sushchik N. N., Dawidowicz P., Gorelysheva Z., Gorniak A., Stroinov Y., Dzialowski A.
Заглавие : Direct and indirect impacts of fish on crustacean zooplankton in experimental mesocosms
Место публикации : Water: MDPI AG, 2019. - Vol. 11, Is. 10. - Ст.2090. - ISSN 20734441 (ISSN) , DOI 10.3390/w11102090
Аннотация: Understanding the factors that regulate phytoplankton and zooplankton is an important goal of aquatic ecologists; however, much remains unknown because of complex interactions between phytoplankton, zooplankton, and fish. Zooplankton, in particular cladocerans, can be regulated by bottom-up factors either via food quantity or food quality in terms of polyunsaturated fatty acids (PUFA) or phosphorus (P) contents in phytoplankton. Fish can recycle nutrients and in turn change the PUFA and P contents of algal resources, thus modifying bottom-up regulation. Furthermore, fish can change phytoplankton structure through consumption of cladocerans which selectively graze phytoplankton. We conducted a mesocosm (300 L) experiment to determine how trophic state and fish affected crustacean dynamics. The mesocosms were filled with water containing natural plankton from the eutrophic Lake Jorzec and mesotrophic Lake Majcz (Northeastern Poland), and we manipulated fish presence/absence. We also conducted a complementary life-table experiment to determine how trophic state and fish nonconsumptively affected demographic parameters of the dominant cladocerans in the mesocosms. Small and large cladoceran species responded differently to food quantity and quality. Small-bodied Ceriodaphnia were regulated mainly by resource concentrations (i.e., food quantity), while large species were limited by PUFAs (i.e., food quality). Fish likely increased food quality in terms of PUFA, primarily eicosapentaenoic acids (EPA), thus providing conditions for more successful development of Daphnia than in the fish-free treatments. Phosphorus in the seston was likely limiting for zooplankton. However, food quality in terms of phosphorus was likely less important than PUFA because zooplankton can accumulate nutrients in their body. © 2019 by the authors.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zotina T. A., Trofimova E. A., Dementyev D. V.
Заглавие : Time-dependent trends of artificial radionuclides in biota of the Yenisei River (Siberia, Russia)
Место публикации : J. Environ. Radioact.: Elsevier Ltd, 2019. - Vol. 208-209. - Ст.106028. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2019.106028
Аннотация: We investigated time-dependent trends of artificial radionuclides in aquatic moss, zoobenthos (amphipods and caddisfly larvae), and three abundant wild fish species (Northern pike, Arctic grayling, and Siberian dace) inhabiting the Yenisei River in the vicinity of the radioactive discharge site in 2007–2015, in a period before and after the shutdown of the last nuclear reactor plant at the Mining-and-Chemical Combine (MCC), which occurred in 2010. From our research, we learned that concentrations of short-lived radionuclides, whose discharges to the Yenisei either stopped or declined after the shutdown of the reactor plant at the MCC (24Na, 46Sc,51Cr, 54Mn, 58Co, 59Fe, 60Co, 65Zn, 103Ru, 141,144Ce, 152,154Eu, 239Np), decreased in biota samples as well. The ecological half-life (EHL) of 65Zn (0.4–0.7 y) was similar to the physical half-life of this isotope, the EHLs of 60Co (1.2–2.1 y) and 152Eu (1.8 y) were shorter than the physical half-lives of these isotopes. Concentration of 137Cs did not decrease significantly in biota of the Yenisei after the shutdown of the last reactor plant because the discharges of this radionuclide to the Yenisei continued at the same level. On a longer-term scale (since 1973 and since 1991), concentration of 137Cs in fish muscle had significantly decreased, following the decrease in annual discharges of this radionuclide to the Yenisei, and the EHL of 137Cs was estimated as 6.5–12.8 y. Statistically significant correlation with annual discharges of 137Cs was revealed for the concentration of this radionuclide in grayling (whole bodies and muscle); dace (muscle), and amphipods. Despite their ability to accumulate high concentrations of 137Cs, aquatic moss and caddisfly larvae (analyzed together with their stony casings) were not sensitive to interannual fluctuations in the releases of this radionuclide to the Yenisei. Among the analyzed fish species of the Yenisei, the highest activity concentration of 137Cs was revealed in pike (body and muscle), indicating biomagnification of this radionuclide in the top level of the trophic chain. © 2019 Elsevier Ltd
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bartsev S. I., Pochekutov A. A.
Заглавие : An elementary multistage discrete model of soil organic matter transformations with a continuous scale of stability
Место публикации : Ecol. Model.: Elsevier B.V., 2019. - Vol. 393. - С. 61-65. - ISSN 03043800 (ISSN) , DOI 10.1016/j.ecolmodel.2018.12.012
Ключевые слова (''Своб.индексиров.''): kinetics of soil organic matter transformations--model of soil organic matter transformations--soil organic matter--biogeochemistry--biological materials--decay (organic)--organic compounds--soils--continuous scale--discrete modeling--elementary model--law of mass action--multistage process--realistic model--soil organic matters--transformation process--mathematical transformations--biotransformation--chemical alteration--decomposition--numerical model--reaction kinetics--soil organic matter
Аннотация: The proposed elementary mathematical model of formation and decomposition of soil organic matter (SOM) is based on using equations of chemical kinetics to describe the multistage process of SOM transformation. The model both describes each step of transformation in accordance with the law of mass action and postulates the trend of increasing stability of the matter towards further transformation, which is common for all steps. Analysis of the model demonstrates that it is extremely difficult to construct a realistic model of SOM dynamics by assembling elementary models of the type presented in this study into the full description of SOM transformation processes. © 2018 Elsevier B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Markova, Svetlana V., Larionova, Marina D., Vysotski, Eugene S.
Заглавие : Shining Light on the Secreted Luciferases of Marine Copepods: Current Knowledge and Applications.
Место публикации : Photochemistry and photobiology. - 2018. - ISSN 1751-1097, DOI 10.1111/php.13077
Аннотация: Copepod luciferases - a family of small secretory proteins of 18.4-24.3 kDa, including a signal peptide, are responsible for bright secreted bioluminescence of some marine copepods. The copepod luciferases use coelenterazine as a substrate to produce blue light in a simple oxidation reaction without any additional cofactors. They do not share sequence or structural similarity with other identified bioluminescent proteins including coelenterazine-dependent Renilla and Oplophorus luciferases. The small size, strong luminescence activity and high stability, including thermostability, make secreted copepod luciferases very attractive candidates as reporter proteins which are particularly useful for nondisruptive reporter assays and for high-throughput format. The most known and extensively investigated representatives of this family are the first cloned GpLuc and MLuc luciferases from copepods Gaussia princeps and Metridia longa, respectively. Immediately after cloning these homologous luciferases were successfully applied as bioluminescent reporters in vivo and in vitro, and since then the scope of their applications continues to grow. This review is an attempt to systemize and critically evaluate the data scattered through numerous articles regarding the main structural features of copepod luciferases, their luminescent and physicochemical properties. We also review the main trends of their application as bioluminescent reporters in cell and molecular biology. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kotlobay A. A., Sarkisyan K. S., Mokrushina Y. A., Marcet-Houben M., Serebrovskaya E. O., Markina N. M., Somermeyer L. G., Gorokhovatsky A. Y., Vvedensky A., Purtov K. V., Petushkov V. N., Rodionova N. S., Chepurnyh T. V., Fakhranurova L. I., Guglya E. B., Ziganshin R., Tsarkova A. S., Kaskova Z. M., Shender V., Abakumov M., Abakumova T. O., Povolotskaya I. S., Eroshkin F. M., Zaraisky A. G., Mishin A. S., Dolgov S. V., Mitiouchkina T. Y., Kopantzev E. P., Waldenmaier H. E., Oliveira A. G., Oba Y., Barsova E., Bogdanova E. A., Gabaldon T., Stevani C. V., Lukyanov S., Smirnov I. V., Gitelson J. I., Kondrashov F. A., Yampolsky I. V.
Заглавие : Genetically encodable bioluminescent system from fungi
Место публикации : Proc. Natl. Acad. Sci. U. S. A.: National Academy of Sciences, 2018. - Vol. 115, Is. 50. - С. 12728-12732. - ISSN 00278424 (ISSN) , DOI 10.1073/pnas.1803615115
Ключевые слова (''Своб.индексиров.''): bioluminescence--fungal luciferase--fungal luciferin biosynthesis
Аннотация: Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually oriented functions from attracting mates to scaring off predators. Half a dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which is found only in bacteria. Here, we report identification of the fungal luciferase and three other key enzymes that together form the biosynthetic cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite. Introduction of the identified genes into the genome of the yeast Pichia pastoris along with caffeic acid biosynthesis genes resulted in a strain that is autoluminescent in standard media. We analyzed evolution of the enzymes of the luciferin biosynthesis cycle and found that fungal bioluminescence emerged through a series of events that included two independent gene duplications. The retention of the duplicated enzymes of the luciferin pathway in nonluminescent fungi shows that the gene duplication was followed by functional sequence divergence of enzymes of at least one gene in the biosynthetic pathway and suggests that the evolution of fungal bioluminescence proceeded through several closely related stepping stone nonluminescent biochemical reactions with adaptive roles. The availability of a complete eukaryotic luciferin biosynthesis pathway provides several applications in biomedicine and bioengineering. © 2018 National Academy of Sciences. All Rights Reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kirillova M. A., Ranjan R., Esimbekova E. N., Kratasyuk V. A.
Заглавие : Role of Hsp90 and ATP in modulating apyrase activity and firefly luciferase kinetics
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Vol. 131. - С. 691-696. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.03.110
Ключевые слова (''Своб.индексиров.''): bioluminescence--heat shock protein 90--high-throughput screening--adenosine triphosphate--apyrase--bovine serum albumin--firefly luciferase--heat shock protein 90--stabilizing agent--article--bioluminescence--clinical study--conformation--controlled study--denaturation--enzyme activity--enzyme kinetics--high throughput screening--incubation time--nonhuman--protein protein interaction--protein refolding--temperature--thermal denaturation--time
Аннотация: The present manuscript describes a novel bioassay consisting of apyrase and heat shock protein 90 (Hsp90) without additional co-chaperone supplementation; intended for high-throughput screening of anti-cancer drugs and prognosis of stress. In this regard, Hsp90 and adenosine 5?-triphosphate (ATP) mediated firefly luciferase (FLuc) kinetics was investigated using apyrase and FLuc as client proteins. Bioluminescent assay containing Hsp90, ATP, and apyrase led to complete loss of luminescence at 50 °C which indicates the protective role of Hsp90 against thermal denaturation. Similarly, the assay sample comprising Hsp90, ATP, and FLuc showed 2 fold increments in luminescence than their counterparts. Introduction of bovine serum albumin (BSA) to the pre-incubated assay mixture led to an initial rise in the luminescence (28%) in comparison to the sample containing Hsp90, ATP and FLuc. Therefore, FLuc based HTS assays are not suitable for clinical samples which may contain stabilizing agents. However, thermally denatured FLuc and apyrase could not regain their active conformation even when Hsp90 and ATP were introduced in the assay system. This observation justifies the role of Hsp90 to be protective rather than a reparation agent when acts without co-chaperones. © 2019
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shershneva A. M., Murueva A. V., Zhila N. O., Volova T. G.
Заглавие : Antifungal activity of P3HB microparticles containing tebuconazole
Место публикации : J. Environ. Sci. Health Part B Pestic. Food Contamin. Agric. Wastes: Taylor and Francis Inc., 2019. - Article in press. - ISSN 03601234 (ISSN) , DOI 10.1080/03601234.2018.1550299
Ключевые слова (''Своб.индексиров.''): antifungal activity--encapsulation--microparticles--poly-3-hydroxybutyrate--tebuconazole--agricultural chemicals--emulsification--encapsulation--fungicides--anti-fungal activity--average diameter--encapsulation efficiency--fusarium moniliforme--micro-particles--phytopathogenic fungi--poly-3-hydroxybutyrate--tebuconazole--fungi
Аннотация: In this study, tebuconazole (TEB)-loaded poly-3-hydroxybutyrate (P3HB)-based microparticles were developed and comprehensively characterized. TEB-loaded microparticles with the initial loading amounts of the fungicide of 10, 25, and 50% of the polymer mass (TEB 10, TEB 25, and TEB 50%) were prepared using emulsion technique. Encapsulation efficiency of TEB varied from 59 to 86%. As the loading amount was increased, the average diameter of microparticles increased too, from 41.3 to 71.7 µm, while zeta potential was not influenced by TEB loading, varying between –32.6 and –35.7 mV. TEB was gradually released from the microparticles to the model medium, and after 60 d, from 25 to 43% of TEB was released depending on the content of the encapsulated fungicide. The data obtained from in vitro TEB release were fitted to different mathematical models. It was shown that the release profiles of TEB could be best explained by the Zero-order, Higuchi, and Hixson–Crowell models. The antifungal activity of the P3HB/TEB microparticles against phytopathogenic fungi Fusarium moniliforme and Fusarium solani was demonstrated by in vitro tests conducted in Petri dishes. Thus, hydrophobic agrochemicals (TEB) can be effectively encapsulated into P3HB microparticles to construct slow-release formulations. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Surmenev R. A., Chernozem R. V., Syromotina D. S., Oehr C., Baumbach T., Krause B., Boyandin A. N., Dvoinina L. M., Volova T. G., Surmeneva M. A.
Заглавие : Low-temperature argon and ammonia plasma treatment of poly-3-hydroxybutyrate films: Surface topography and chemistry changes affect fibroblast cells in vitro
Место публикации : Eur Polym J: Elsevier Ltd, 2019. - Vol. 112. - С. 137-145. - ISSN 00143057 (ISSN) , DOI 10.1016/j.eurpolymj.2018.12.040
Ключевые слова (''Своб.индексиров.''): cell adhesion--dielectric barrier discharge--plasma treatment--surface etching--wetting behaviour--ammonia--argon--cell adhesion--cell culture--chemical modification--contact angle--dielectric materials--electric discharges--fibroblasts--mammals--nitrogen plasma--plasma applications--surface roughness--surface treatment--temperature--topography--ammonia plasma treatment--dielectric barrier discharges--different proportions--plasma treatment--poly-3-hydroxybutyrate--poly3-hydroxybutyrate (phb)--surface etching--wetting behaviour--wetting
Аннотация: Poly-3-hydroxybutyrate (PHB) films were plasma-treated using pure NH3, pure Ar or mixtures of the two different proportions (20%, 30%, 40%, 50%, 70% NH3 in Ar). Surface chemistry and surface topography changes of PHB films were observed after plasma processing in all plasma regimes. The XPS results confirmed the absence of chemical modification in the case of pure Ar plasma treatment. Nitrogen-containing groups (e.g., N–C[dbnd]O) were detected on the surfaces of P3HB films treated with NH3-containing plasma. The surfaces of the untreated P3HB films were hydrophobic, and plasma treatment turned the surfaces hydrophilic, irrespective of the treatment. A significant decrease in the contact angle and an increase in the free surface energy were observed. An insignificant surface ageing effect was observed when P3HB samples were exposed to air for 10 days. In NIH 3T3 mice fibroblast cells, cell adhesion was significantly improved after plasma treatment in an Ar atmosphere, which is likely related to the fact that there was a surface ? potential of 88.6 mV at neutral pH, causing a cleavage of the polymer chains and an increase in surface roughness. © 2018 Elsevier Ltd
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Rozhko T. V., Kolesnik O. V., Badun G. A., Stom D. I., Kudryasheva N. S.
Заглавие : Humic substances mitigate the impact of tritium on luminous marine bacteria. Involvement of reactive oxygen species
Место публикации : International Journal of Molecular Sciences: MDPI AG, 2020. - Vol. 21, Is. 18. - Ст.6783. - С. 1-12. - ISSN 16616596 (ISSN), DOI 10.3390/ijms21186783
Аннотация: The paper studies the combined effects of beta-emitting radionuclide tritium and Humic Substances (HS) on the marine unicellular microorganism—luminous bacteria—under conditions of low-dose radiation exposures (0.04 Gy). Tritium was used as a component of tritiated water. Bacterial luminescence intensity was considered as a tested physiological parameter. The bioluminescence response of the marine bacteria to tritium corresponded to the “hormesis” model: it included stages of bioluminescence inhibition and activation, as well as the absence of the effect. HS were shown to decrease the inhibition and activation effects of tritium, similar to those of americium-241, alpha-emitting radionuclide, studied earlier. Correlations between the bioluminescence intensity and the content of Reactive Oxygen Species (ROS) were found in the radioactive bacterial suspensions. The results demonstrate an important role of HS in natural processes in the regions of low radioactive contamination: HS can mitigate radiotoxic effects and adaptive response of microorganisms to low-dose radioactive exposures. The involvement of ROS in these processes was demonstrated. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Semyonov, Denis A., Eltsov, Ilia, V, Nechipurenko, Yury D.
Заглавие : A New Bias Site for Epigenetic Modifications: How Non-Canonical GC Base Pairs Favor Mechanochemical Cleavage of DNA
Колич.характеристики :8 с
Коллективы : program of the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology; Program of Fundamental Research for State Academies for years 2013-2020 [01201363818]; Russian Ministry of Science and High Education [05.604.21.0234, RFMEFI60419X0234]
Место публикации : Bioessays: WILEY, 2020. - Vol.: 42, Issue: 11. - Ст.2000051. - ISSN 0265-9247, DOI 10.1002/bies.202000051. - ISSN 1521-1878(eISSN)
Примечания : Cited References:55. - The authors thank Dr. Dmitry M. Graifer, Prof. Vladimir A. Reznikov, and Dr. Anastasia Anashkina, for useful discussion. The authors thank the Prof. D. R. Forsdyke whose advice on improving the style of the manuscript was very useful. This work was supported by the program of the Presidium of the Russian Academy of Sciences for Molecular and Cellular Biology and the Program of Fundamental Research for State Academies for years 2013-2020, project no. 01201363818. The work was supported by the Russian Ministry of Science and High Education (grant #05.604.21.0234, unique number RFMEFI60419X0234)
Предметные рубрики: NUCLEIC-ACIDS
CYTOSINE
MUTAGENESIS
N-4-METHOXYCYTOSINE
Аннотация: Properties of non-canonical GC base pairs and their relations with mechanochemical cleavage of DNA are analyzed. A hypothesis of the involvement of the transient GC wobble base pairs both in the mechanisms of the mechanochemical cleavage of DNA and epigenetic mechanisms involving of 5-methylcytosine, is proposed. The hypothesis explains the increase in the frequency of the breaks of the sugar-phosphate backbone of DNA after cytosines, the asymmetric character of these breaks, and an increase in break frequency in CpG after cytosine methylation. As an alternative hypothesis, probable implication of GC(+)Hoogsteen base pairs is considered, which now exemplify the best-studied non-canonical GC base pairs in the DNA double helix. Also see the video abstract here https://youtu.be/EUunVWL0ptw.
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https://onlinelibrary.wiley.com/doi/epdf/10.1002/bies.202000051
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15.

Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Bartsev S., Baturina P.
Заглавие : A heuristic neural network model in the research of properties of evolutionary trajectories
Колич.характеристики :7 с
Место публикации : INTERNATIONAL WORKSHOP ADVANCED TECHNOLOGIES IN MATERIAL SCIENCE,: IOP PUBLISHING LTD, 2019. - Vol. 537: International Workshop on Advanced Technologies in Material Science, (APR 04-06, 2019, Krasnoyarsk, RUSSIA). - Ст.042001. - (IOP Conference Series-Materials Science and Engineering). - , DOI 10.1088/1757-899X/537/4/042001
Примечания : Cited References:21
Предметные рубрики: SEQUENCE SPACE
Аннотация: There is considerable data on molecular evolution, but there remains no approach to systematizing them within the framework of the key problems of biology. To search for the most common properties of evolving systems, the heuristic method has been proposed. Artificial networks of formal neurons were chosen as the heuristic model object. The paper examines the divergent component of evolutionary trajectory formation. As a result of the simulation, the dependence of the potential variability parameter on the position of the fitness function landscape was obtained. The simulation results are in agreement with the real data of molecular evolution experiments.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Fefilova, Elena, Dubovskaya, Olga, Frolova, Larisa, Abramova, Ekaterina, Kononova, Olga, Nigamatzyanova, Gulnara, Zuev, Ivan, Kochanova, Elena
Заглавие : Biogeographic patterns of planktonic and meiobenthic fauna diversity in inland waters of the Russian Arctic
Колич.характеристики :17 с
Коллективы : Federal Tasks of Department of Animals Ecology of the Institute of Biology of Komi Science Centre of the Ural Branch of the Russian Academy of Sciences [AAAA-A17-117112850235-2]; Institute of Biophysics of Federal Research Center "Krasnoyarsk Science Center" of Siberian Branch of Russian Academy of Sciences [51.1.1]; Siberian Federal University [FSRZ-2020-0006]
Место публикации : Freshw. Biol.: WILEY, 2020. - Article in press. - ISSN 0046-5070, DOI 10.1111/fwb.13624. - ISSN 1365-2427(eISSN)
Примечания : Cited References:63. - We would like to thank A. Kotov, N. Korovchinsky, A. Sinev, E. Bekker, N. Smirnov (all from Severtsov Institute of Ecology and Evolution of RAS) for their assistance in Cladocera identification. We are very grateful to Jennifer Lento (University of New Brunswick, Canada) for helping us obtain elevation, temperature, and precipitation data from World Climate and ArcticDEM (NGA-NSF). We are also grateful to Willem Goedkoop for helpful comments on an earlier version of the manuscript. The study was performed in part as Federal Tasks of Department of Animals Ecology of the Institute of Biology of Komi Science Centre of the Ural Branch of the Russian Academy of Sciences (AAAA-A17-117112850235-2), and also of Institute of Biophysics of Federal Research Center "Krasnoyarsk Science Center" of Siberian Branch of Russian Academy of Sciences (project No. 51.1.1) and the Siberian Federal University (project No. FSRZ-2020-0006). Monitoring investigations in the Lena River Delta were conducted under the framework of Russian-German, "Lena" expeditions (Alfred Wegener Institute, Potsdam, Germany) with logistic and technical support of Scientific Research Station "Samoylov Island" (Trofimuk Institute of Petroleum-Gas, Geology and Geophysics SB RAS, Novosibirsk). We are grateful to three anonymous reviewers, Guest Editor, Dr Joseph Culp, and the Chief Editor, Prof. Belinda Robson for their useful comments to improve the manuscript.
Предметные рубрики: GLOBAL DIVERSITY
CRUSTACEAN ZOOPLANKTON
CLADOCERA
ANOMOPODA
Аннотация: Broad-scale assessment of biodiversity is needed for detection of future changes across substantial regions of the Arctic. Presently, there are large data and information gaps in species composition and richness of the freshwater planktonic and meiobenthos communities of the Russian Arctic. Analysis of these data is very important for identifying the spatial distribution and temporal changes in species richness and diversity of rotifers, cladocerans, and copepods in the continental Russian Arctic. We investigated biogeographic patterns of freshwater plankton and meiobenthos fromc. 67 degrees to 73 degrees N by analysing data over the period 1960-2017. These data include information on the composition of rotifers, cladocerans, and copepods obtained from planktonic and meiobenthic samples, as well as from subfossil remains in bottom sediments of seven regions from the Kola Peninsula in the west, to the Indigirka River Basin (east Siberia) in the east. Total richness included 175 species comprised of 49 rotifer genera, 81 species from 40 cladoceran genera, and 101 species from 42 genera of calanoid, cyclopoid, and harpacticoid copepods. Longitudinal trends in rotifer and micro-crustacean diversity were revealed by change in species composition from Europe to eastern Siberia. The most common and widespread species were 19 ubiquitous taxa that includedKellicottia longispina(Rotifera),Chydorus sphaericuss. lat. (Cladocera),Heterocope borealis,Acanthocyclops vernalis, andMoraria duthiei(Copepoda). The highest number of rare species was recorded in the well-studied region of the Bolshezemelskaya tundra and in the Putorana Plateau. The total number of copepod and rotifer species in both Arctic lakes and ponds tended to increase with latitude. Relative species richness of copepods was positively associated with waterbody area, elevation, and precipitation, while relative species richness of cladocerans was positively related to temperature. This result is consistent with known thermophilic characteristics of cladocerans and the cold tolerance properties of copepods, with the former being dominant in shallow, warmer waterbodies of some western regions, and the latter being dominant in large cold lakes and waterbodies of eastern regions. Rotifers showed a negative association with these factors. Alpha- and beta-diversity of zooplankton in the Russian Arctic were strongly related to waterbody type. Lake zooplankton communities were more diverse than those in pond and pool systems. Moreover, the highest beta-diversity values were observed in regions that showed a greater breadth in latitude and highly heterogeneous environmental conditions and waterbody types (Bolshezemelskaya tundra and Putorana Plateau). Redistribution of freshwater micro-fauna caused by human activities occurred in the 1990s and 2000s. As a result of climate warming, a few cladoceran species appear to have extended their range northward. Nevertheless, the rotifer and micro-crustacean fauna composition and diversity of the majority of Arctic regions generally remain temporally conservative, and spatial differences in composition and species richness are chiefly associated with the differences between the warmer European and colder east Siberian climates.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya V. V., Bashmakova E. E., Frank L. A.
Заглавие : Coelenterazine-dependent luciferases as a powerful analytical tool for research and biomedical applications
Место публикации : Int. J. Mol. Sci.: MDPI AG, 2020. - Vol. 21, Is. 20. - Ст.7465. - С. 1-31. - ISSN 16616596 (ISSN), DOI 10.3390/ijms21207465
Аннотация: The functioning of bioluminescent systems in most of the known marine organisms is based on the oxidation reaction of the same substrate—coelenterazine (CTZ), catalyzed by luciferase. Despite the diversity in structures and the functioning mechanisms, these enzymes can be united into a common group called CTZ-dependent luciferases. Among these, there are two sharply different types of the system organization—Ca2+-regulated photoproteins and luciferases themselves that function in accordance with the classical enzyme–substrate kinetics. Along with deep and comprehensive fundamental research on these systems, approaches and methods of their practical use as highly sensitive reporters in analytics have been developed. The research aiming at the creation of artificial luciferases and synthetic CTZ analogues with new unique properties has led to the development of new experimental analytical methods based on them. The commercial availability of many ready-to-use assay systems based on CTZ-dependent luciferases is also important when choosing them by first-time-users. The development of analytical methods based on these bioluminescent systems is currently booming. The bioluminescent systems under consideration were successfully applied in various biological research areas, which confirms them to be a powerful analytical tool. In this review, we consider the main directions, results, and achievements in research involving these luciferases. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Scopus
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Fefilova E., Dubovskaya O., Frolova L., Abramova E., Kononova O., Nigamatzyanova G., Zuev I., Kochanova E.
Заглавие : Biogeographic patterns of planktonic and meiobenthic fauna diversity in inland waters of the Russian Arctic
Место публикации : Freshw. Biol.: Blackwell Publishing Ltd, 2020. - Article in press. - ISSN 00465070 (ISSN), DOI 10.1111/fwb.13624
Аннотация: Broad-scale assessment of biodiversity is needed for detection of future changes across substantial regions of the Arctic. Presently, there are large data and information gaps in species composition and richness of the freshwater planktonic and meiobenthos communities of the Russian Arctic. Analysis of these data is very important for identifying the spatial distribution and temporal changes in species richness and diversity of rotifers, cladocerans, and copepods in the continental Russian Arctic. We investigated biogeographic patterns of freshwater plankton and meiobenthos from c. 67° to 73°N by analysing data over the period 1960–2017. These data include information on the composition of rotifers, cladocerans, and copepods obtained from planktonic and meiobenthic samples, as well as from subfossil remains in bottom sediments of seven regions from the Kola Peninsula in the west, to the Indigirka River Basin (east Siberia) in the east. Total richness included 175 species comprised of 49 rotifer genera, 81 species from 40 cladoceran genera, and 101 species from 42 genera of calanoid, cyclopoid, and harpacticoid copepods. Longitudinal trends in rotifer and micro-crustacean diversity were revealed by change in species composition from Europe to eastern Siberia. The most common and widespread species were 19 ubiquitous taxa that included Kellicottia longispina (Rotifera), Chydorus sphaericus s. lat. (Cladocera), Heterocope borealis, Acanthocyclops vernalis, and Moraria duthiei (Copepoda). The highest number of rare species was recorded in the well-studied region of the Bolshezemelskaya tundra and in the Putorana Plateau. The total number of copepod and rotifer species in both Arctic lakes and ponds tended to increase with latitude. Relative species richness of copepods was positively associated with waterbody area, elevation, and precipitation, while relative species richness of cladocerans was positively related to temperature. This result is consistent with known thermophilic characteristics of cladocerans and the cold tolerance properties of copepods, with the former being dominant in shallow, warmer waterbodies of some western regions, and the latter being dominant in large cold lakes and waterbodies of eastern regions. Rotifers showed a negative association with these factors. Alpha- and ?-diversity of zooplankton in the Russian Arctic were strongly related to waterbody type. Lake zooplankton communities were more diverse than those in pond and pool systems. Moreover, the highest ?-diversity values were observed in regions that showed a greater breadth in latitude and highly heterogeneous environmental conditions and waterbody types (Bolshezemelskaya tundra and Putorana Plateau). Redistribution of freshwater micro-fauna caused by human activities occurred in the 1990s and 2000s. As a result of climate warming, a few cladoceran species appear to have extended their range northward. Nevertheless, the rotifer and micro-crustacean fauna composition and diversity of the majority of Arctic regions generally remain temporally conservative, and spatial differences in composition and species richness are chiefly associated with the differences between the warmer European and colder east Siberian climates. © 2020 John Wiley & Sons Ltd.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pande S., Ranjan R., Shuvaev A. N., Malinovskaya N. A., Ryazanova M., Salmina A. B., Kolenchukova O. A., Kratasyuk V. A.
Заглавие : Dietary buckwheat enhances sirtuin1 without calorie restriction
Место публикации : J. Cereal Sci.: Academic Press, 2020. - Vol. 94. - Ст.103004. - ISSN 07335210 (ISSN), DOI 10.1016/j.jcs.2020.103004
Аннотация: In the present investigation, the role of dietary intervention in male Wistar rats (n = 8, 3 groups) was studied to observe absolute sirtuin 1 (SIRT1) levels (expressed as ng mg?1 total protein) in serum, stomach, liver, and kidney. Dietary buckwheat at 30% (w/w) level of incorporation in the standard diet (Buckwheat Enriched Diet, BED) improved SIRT1 with values 0.933 ± 0.05, 210 ± 7, 63.26 ± 4, and 69.89 ± 3 in serum, stomach, liver, and kidney respectively when compared to the respective control values of 0.536 ± 0.03, 156 ± 23.3, 31.07 ± 2 and 47.11 ± 4. Moreover, BED though isocaloric to CR diet, led to weight gain (g) by 63.11 ± 3.8, ca. 10%, and 40% higher than control (56.27 ± 5.6) and CR (45.05 ± 4.1) diet groups. A marked rise in Feed Efficiency Ratio (FER) by ca. 37% while a 30% decrease in Feed Conversion Ratio (FCR) was observed for the BED group which supports unexpected weight gain in rats post-dietary intervention. The results justify the superior nutritional profile of buckwheat laden with essential nutrients, essential proteins, and bioactives. In contrast, Calorie Restriction (CR) resulted in a decline of the total protein content in circulation by 19%, while reduction of total protein in stomach, liver, and kidney was estimated to be 95%, 35.2%, and 27% respectively though SIRT1 values were comparatively the highest in all the samples studied. A 30-fold enhancement of SIRT1 in stomach post CR is presumed to counter enhanced stress in gastric tissues. Therefore, mild to moderate expression of SIRT1 may confer beneficial effects such as delayed aging and stress resistance but exceedingly high SIRT1 may evoke increased oxidative stress. © 2020 Elsevier Ltd
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakova A. A., Kolmakov, V., I
Заглавие : Amino Acid Composition of Green Microalgae and Diatoms, Cyanobacteria, and Zooplankton (Review)
Колич.характеристики :10 с
Коллективы : State task of the basic research program of the Russian Federation [VI.51.S]
Место публикации : Inland Water Biol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2019. - Vol. 12, Is. 4. - С. 452-461. - ISSN 1995-0829, DOI 10.1134/S1995082919040060. - ISSN 1995-0837(eISSN)
Примечания : Cited References:72. - State task of the basic research program of the Russian Federation, topic number VI.51.S.
Предметные рубрики: BIOCHEMICAL-COMPOSITION
PROTEIN-CONTENT
CRUSTACEAN ZOOPLANKTON
Аннотация: We have reviewed foreign and domestic literature devoted to the study of the amino acid (AA) composition of aquatic organisms representing major groups of producers (green microalgae and diatoms, and cyanobacteria) and primary consumers (zooplankton). Based on published data, we estimate the composition of essential and nonessential AAs of microalgae, cyanobacteria, and zooplankton and determine their differences. It is concluded that the AA composition of major groups of plankton is heterogeneous. The role of AAs as a limiting factor for the development of herbivorous zooplankton is discussed. The prospects and the need for further study of AA composition in order to develop a complete theory of functioning of aquatic ecosystems have been demonstrated.
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