Главная
Авторизация
Фамилия
Пароль
 

Базы данных


Труды сотрудников ИБФ СО РАН - результаты поиска

Вид поиска
Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН
Иностранные журналы библиотеки Института биофизики СО РАН
Отечественные журналы библиотеки Института биофизики СО РАН
Каталог диссертаций ИБФ СО РАН
Труды сотрудников ИБФ СО РАН
Область поиска
Формат представления найденных документов:
полныйинформационныйкраткий
Отсортировать найденные документы по:
авторузаглавиюгоду изданиятипу документа
Поисковый запрос: (<.>K=Biomedicine<.>)
Общее количество найденных документов : 9
Показаны документы с 1 по 9
1.


   
    Polyhydroxyalkanoates (PHA) for therapeutic applications / J. Y. Zhang [et al.] // Mater. Sci. Eng. C-Mater. Biol. Appl. - 2018. - Vol. 86. - P144-150, DOI 10.1016/j.msec.2017.12.035. - Cited References:105. - This research was financially supported by a grant from Ministry of Sciences and Technology (Grant No. 2016YFB0302504); grants from National Natural Science Foundation of China (Grant No. 31430003, 31600072 and 81503079); a grant from Natural Science Foundation of Jiangxi Province (Grant No. 20161BAB215204); and a grant from Russian Science Foundation (Grant No. 17-15-01352). Tsinghua President Fund also supported this project (Grant No. 2015THZ10). . - ISSN 0928-4931. - ISSN 1873-0191
РУБ Materials Science, Biomaterials
Рубрики:
TISSUE ENGINEERING APPLICATIONS
   3-HYDROXYBUTYRATE METHYL-ESTER
Кл.слова (ненормированные):
PHA -- Therapeutics -- Tissue engineering -- Implants -- Drug delivery -- Biomedicine
Аннотация: As intracellular carbon and energy storage materials, polyhydroxyalkanoates (PHA) are a diverse biopolyesters synthesized by many bacteria. PHA have been produced in large quantity for various application research including medical implants for approximately 30 years. Many studies demonstrated that PHA are promising implant materials due to their diverse and ascendant mechanical, biodegradable and tissue compatible properties. Importantly, common PHA biodegradation products including oligomers and monomers are also not toxic to the cells and tissues. Pharmaceutical applications of some PHA degradation products also have been reported. So far, no study has been reported to have any carcinogenesis result induced by any PHA or their biodegradation products. All results suggest that PHA could be developed into various bio-implant products.

WOS,
Смотреть статью
Держатели документа:
Nanchang Univ, Inst Life Sci, Lab Fear & Anxiety Disorders, Nanchang 330031, Jiangxi, Peoples R China.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Tsinghua Univ, Sch Life Sci, MOE Key Lab Bioinformat, Beijing 100084, Peoples R China.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, BR-05509900 Sao Paulo, Brazil.

Доп.точки доступа:
Zhang, Junyu; Shishatskaya, Ekaterina I.; Volova, Tatiana G.; da Silva, Luiziana Ferreira; Chen, Guo-Qiang; Ministry of Sciences and Technology [2016YFB0302504]; National Natural Science Foundation of China [31430003, 31600072, 81503079]; Natural Science Foundation of Jiangxi Province [20161BAB215204]; Russian Science Foundation [17-15-01352]; Tsinghua President Fund [2015THZ10]

Найти похожие
2.


   
    Genetically encodable bioluminescent system from fungi / A. A. Kotlobay [et al.] // Proc. Natl. Acad. Sci. U. S. A. - 2018. - Vol. 115, Is. 50. - P12728-12732, DOI 10.1073/pnas.1803615115 . - ISSN 0027-8424
Кл.слова (ненормированные):
Bioluminescence -- Fungal luciferase -- Fungal luciferin biosynthesis
Аннотация: Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually oriented functions from attracting mates to scaring off predators. Half a dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which is found only in bacteria. Here, we report identification of the fungal luciferase and three other key enzymes that together form the biosynthetic cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite. Introduction of the identified genes into the genome of the yeast Pichia pastoris along with caffeic acid biosynthesis genes resulted in a strain that is autoluminescent in standard media. We analyzed evolution of the enzymes of the luciferin biosynthesis cycle and found that fungal bioluminescence emerged through a series of events that included two independent gene duplications. The retention of the duplicated enzymes of the luciferin pathway in nonluminescent fungi shows that the gene duplication was followed by functional sequence divergence of enzymes of at least one gene in the biosynthetic pathway and suggests that the evolution of fungal bioluminescence proceeded through several closely related stepping stone nonluminescent biochemical reactions with adaptive roles. The availability of a complete eukaryotic luciferin biosynthesis pathway provides several applications in biomedicine and bioengineering. © 2018 National Academy of Sciences. All Rights Reserved.

Scopus,
Смотреть статью,
WOS
Держатели документа:
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russian Federation
Planta LLC, Moscow, 121205, Russian Federation
Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria
Medical Research Council London Institute of Medical Sciences, Imperial College London, London, W12 0NN, United Kingdom
Centre for Genomic Regulation, Barcelona Institute for Science and Technology, Barcelona, 08003, Spain
Universitat Pompeu Fabra, Barcelona, 08003, Spain
Evrogen JSC, Moscow, 117997, Russian Federation
Institute of Biophysics, Federal Research Center Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Moscow, 142290, Russian Federation
Pirogov Russian National Research Medical University, Moscow, 117997, Russian Federation
Biomedical Nanomaterials, National Research Technological University (MISiS), Moscow, 119049, Russian Federation
Skolkovo Institute of Science and Technology, Moscow, 121205, Russian Federation
Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, 05508-000, Brazil
Departamento de Oceanografia Fisica, Quimica e Geologica, Instituto Oceanografico, Universidade de Sao Paulo, Sao Paulo, 05508-120, Brazil
Department of Environmental Biology, Chubu University, Kasugai, 487-8501, Japan
Catalan Institution for Research and Advanced Studies (ICREA), Barcelona, 08010, Spain
Departamento de Quimica Fundamental, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, 05508-000, Brazil

Доп.точки доступа:
Kotlobay, A. A.; Sarkisyan, K. S.; Mokrushina, Y. A.; Marcet-Houben, M.; Serebrovskaya, E. O.; Markina, N. M.; Somermeyer, L. G.; Gorokhovatsky, A. Y.; Vvedensky, A.; Purtov, K. V.; Petushkov, V. N.; Rodionova, N. S.; Chepurnyh, T. V.; Fakhranurova, L. I.; Guglya, E. B.; Ziganshin, R.; Tsarkova, A. S.; Kaskova, Z. M.; Shender, V.; Abakumov, M.; Abakumova, T. O.; Povolotskaya, I. S.; Eroshkin, F. M.; Zaraisky, A. G.; Mishin, A. S.; Dolgov, S. V.; Mitiouchkina, T. Y.; Kopantzev, E. P.; Waldenmaier, H. E.; Oliveira, A. G.; Oba, Y.; Barsova, E.; Bogdanova, E. A.; Gabaldon, T.; Stevani, C. V.; Lukyanov, S.; Smirnov, I. V.; Gitelson, J. I.; Kondrashov, F. A.; Yampolsky, I. V.

Найти похожие
3.


   
    Metal-enhanced luminescence: Current trend and future perspectives- A review / R. Ranjan [et al.] // Anal. Chim. Acta. - 2017. - Vol. 971. - P1-13, DOI 10.1016/j.aca.2017.03.051. - Cited References:124. - The research was partially supported by the Russian Foundation for Basic Research [project No 16-34-60100], the state budget allocated to the fundamental research [project No 01201351504] and Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support Fund to the research project and Russian Foundation for Basic Research [project No 16-44-242126]. . - ISSN 0003-2670. - ISSN 1873-4324
РУБ Chemistry, Analytical
Рубрики:
RESONANCE ENERGY-TRANSFER
   PLASMON-COUPLED EMISSION
Кл.слова (ненормированные):
Nanomaterials -- Surface plasmons -- Luminescence enhancement -- Biosensors
Аннотация: Optically enhanced biosensing strategies are prerequisites for developing miniature and highly sensitive multiplexed analytical platforms. Such smart biosensing systems are highly promising for use in the fields of biomedicine and environmental monitoring. Optical signal enhancement during bioassays is attributed to the complex opto-electronic interactions of incoming photonic signals at the nanomaterial interface. Research on the use of metals other than gold and silver for such purposes tends to extend the spectral window to observe luminescence enhancement effects. Such manifold increase in luminescence may be explained by the principles of plasmon coupling, directional emission led high collection efficiency, Rayleigh scattering and related opto-electronic events. The present review begins with a mechanistic description of important phenomena associated with metal-induced luminescence enhancement, particularly focusing on the origin of metal-enhanced luminescence. This review further analyses the hybrid nanostructure capabilities responsible for maintaining unique opto-electronic properties during bio-functionalisation. Current research trends in this area, future scope of this field for designing useful bioassays and concluding remarks are then discussed. (C) 2017 Elsevier B.V. All rights reserved.

WOS,
Смотреть статью
Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biophys, Lab Bioluminescent Biotechnol, 79 Svobodny Prospect, Krasnoyarsk 660041, Russia.
RAS, SB, Krasnoyarsk Sci Ctr, Inst Biophys,Fed Res Ctr, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Ranjan, Rajeev; Esimbekova, Elena N.; Kirillova, Maria A.; Kratasyuk, Valentina A.; Russian Foundation for Basic Research [16-34-60100, 16-44-242126]; Government of Krasnoyarsk Territory, Krasnoyarsk Region Science and Technology Support Fund; [01201351504]

Найти похожие
4.


   
    Creation of artificial luciferases to expand their analytical potential / L. A. Frank // Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - P919-929 . - ISSN 1386-2073
Кл.слова (ненормированные):
Bioimaging -- Bioluminescence -- Luciferase -- Luciferase-based assay -- Luciferin -- Mutagenesis -- Photoprotein -- Reporter assay
Аннотация: Bioluminescent proteins have been intensively used as high sensitive reporters in all kinds of binding assays (immuno-, nucleic acid hybridization assays, etc.) and in bioimaging. But natural luciferases do not always meet the requirements set for them as the assay reporters: thermostabitity, definite bioluminescence spectral and kinetics characteristics, stability to chemical modifications, etc. Luciferases with different appropriate characteristics as well as various luciferin derivatives were obtained using mutagenesis and chemical synthesis. Thanks to rigorous efforts of many researchers bioluminescencebased analytical techniques offer a great potential for solving analytical tasks in the field of biotechnology, biomedicine, pharmacology, etc. © 2015 Bentham Science Publishers.

Scopus
Держатели документа:
Photobiology Laboratory, Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodnii ave., 79, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Frank, L. A.

Найти похожие
5.


   
    Creation of Artificial Luciferases to Expand their Analytical Potential [Text] / L. A. Frank // Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - P919-929, DOI 10.2174/1386207318666150917100011. - Cited References:79. - The work was supported by: the RFBR grant No. 14-08-00902/14; the State budget allocated to the fundamental research at the Russian Academy of Sciences (project No. VI 57.1.1). . - ISSN 1386-2073. - ISSN 1875-5402
РУБ Biochemical Research Methods + Chemistry, Applied + Pharmacology &
Рубрики:
BIOLUMINESCENT REPORTER APPLICATIONS
   COELENTERAZINE-BINDING PROTEIN
Кл.слова (ненормированные):
Luciferase -- luciferin -- photoprotein -- bioluminescence -- mutagenesis -- luciferase-based assay -- bioimaging -- reporter assay
Аннотация: Bioluminescent proteins have been intensively used as high sensitive reporters in all kinds of binding assays (immuno-, nucleic acid hybridization assays, etc.) and in bioimaging. But natural luciferases do not always meet the requirements set for them as the assay reporters: thermostabitity, definite bioluminescence spectral and kinetics characteristics, stability to chemical modifications, etc. Luciferases with different appropriate characteristics as well as various luciferin derivatives were obtained using mutagenesis and chemical synthesis. Thanks to rigorous efforts of many researchers bioluminescence-based analytical techniques offer a great potential for solving analytical tasks in the field of biotechnology, biomedicine, pharmacology, etc.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Photobiol Lab, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Frank, Ludmila A.; RFBR [14-08-00902/14]; [VI 57.1.1]

Найти похожие
6.


   
    Electrospinning of polyhydroxyalkanoate fibrous scaffolds: effects on electrospinning parameters on structure and properties [Text] / T. . Volova [et al.] // J. Biomater. Sci.-Polym. Ed. - 2014. - Vol. 25, Is. 4. - P370-393, DOI 10.1080/09205063.2013.862400. - Cited References: 52. - This study was financially supported by Project 'Biotechnologies of novel biomaterials: innovative biopolymers and devices for biomedicine' (Agreement No. 1 of 15.02.2013 to Agreement No. 11.G34.31.0013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, 'On measures designed to attract leading scientists to the Russian institutions of higher learning' and Grant of the RF President for supporting young Doctors of Sciences No. MD-3112.2012.4. . - ISSN 0920-5063
РУБ Engineering, Biomedical + Materials Science, Biomaterials + Polymer Science
Рубрики:
TISSUE ENGINEERING APPLICATIONS
   FIBER MATS
   POLY 3-HYDROXYBUTYRATE
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
   BIOCOMPATIBILITY
   PROLIFERATION
   FABRICATION
   NANOFIBERS
   COPOLYMERS
   MEMBRANES
Кл.слова (ненормированные):
electrospinning -- polyhydroxyalkanoates -- ultrafine fibers -- physical-mechanical properties -- fibroblast cells
Аннотация: In this study, electrospinning was used to prepare ultrafine fibers from PHAs with different chemical compositions: P(3HB) and copolymers: P(3HB-co-4HB), P(3HB-co-3HV), and P(3HB-co-3HHx). The main process parameters that influence ultrafine fiber diameter and properties (polymer concentration, solution feeding rate, working distance, and applied voltage) have been investigated and their effects evaluated. The study revealed electrospinning parameters for the production of high-quality ultrafine fibers and determined which parameters should be varied to tailor the properties of the products. This study is the first to compare biological and physical-mechanical parameters of PHAs with different chemical compositions as dependent upon the fractions of monomers constituting the polymers and ultrafine fiber orientation. Mechanical strength of aligned ultrafine fibers prepared from different PHAs is higher than that of randomly oriented ones; no significant effect of ultrafine fiber orientation on surface properties has been found. None of the fibrous scaffolds produced by electrospinning from PHAs had any adverse effects on attachment, growth, and viability of NIH 3T3 mouse fibroblast cells, and all of them were found to be suitable for tissue engineering applications.

WOS,
Scopus
Держатели документа:
[Volova, Tatiana
Sukovatyi, Aleksey
Nikolaeva, Elena] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
[Goncharov, Dmitriy
Shishatskaya, Ekaterina] Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660041, Russia
[Shabanov, Alexander] Russian Acad Sci, LV Kirenskii Inst Phys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН
ИФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Goncharov, D...; Sukovatyi, A...; Shabanov, A...; Nikolaeva, E...; Shishatskaya, E...; Project 'Biotechnologies of novel biomaterials: innovative biopolymers and devices for biomedicine' [1, 11.G34.31.0013]; Government of the Russian Federation [220]; RF President for supporting young Doctors of Sciences [MD-3112.2012.4]

Найти похожие
7.


   
    A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs [Text] / T. . Volova [et al.] // PLoS One. - 2014. - Vol. 9, Is. 2. - Ст. e87551, DOI 10.1371/journal.pone.0087551. - Cited References: 64. - This study was financially supported by Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" (Agreement No. 11.G34.31.0013 with Amendment No. 1 of 15 February 2013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - ISSN 1932-6203
РУБ Multidisciplinary Sciences
Рубрики:
RALSTONIA-EUTROPHA
   BIODEGRADABLE POLYHYDROXYALKANOATES
   AEROMONAS-HYDROPHILA
   ESCHERICHIA-COLI
   MOLECULAR-WEIGHT
   SURFACE-ENERGY
   NORTH PACIFIC
   TERPOLYESTER
   BIOSYNTHESIS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE-CO-3-HYDROXYHEXANOATE)
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.

WOS
Держатели документа:
[Volova, Tatiana
Kiselev, Evgeniy
Nikolaeva, Elena
Sukovatiy, Aleksey
Shishatskaya, Ekaterina] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Volova, Tatiana
Vinogradova, Olga
Shishatskaya, Ekaterina] Siberian Fed Univ, Krasnoyarsk, Russia
[Chistyakov, Anton] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Kiselev, E...; Vinogradova, O...; Nikolaeva, E...; Chistyakov, A...; Sukovatiy, A...; Shishatskaya, E...; Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" [11.G34.31.0013]

Найти похожие
8.


   
    A hybrid PHB-hydroxyapatite composite for biomedical application: Production, in vitro and in vivo investigation / E. I. Shishatskaya, I. A. Khlusov, T. G. Volova // Journal of Biomaterials Science, Polymer Edition. - 2006. - Vol. 17, Is. 5. - P481-498, DOI 10.1163/156856206776986242 . - ISSN 0920-5063
Кл.слова (ненормированные):
Biocompatibility -- Hydroxyapatite (HA) -- PHB-hydroxyapatite composite -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (P(3HB)) -- Properties -- Biocompatibility -- Differential thermal analysis -- Electron microscopy -- Free energy -- Interfacial energy -- Physical properties -- Surface properties -- X ray analysis -- Biomedical application -- Physicochemical properties -- Polyhydroxyalkanoate (PHA) -- Polyhydroxybutyrate (PHB) -- Hydroxyapatite -- hydroxyapatite -- poly(3 hydroxybutyric acid) -- polymer -- biomaterial -- hydroxybutyric acid -- adhesion -- animal cell -- animal tissue -- article -- biomedicine -- bone marrow cell -- cell differentiation -- cell growth -- chemical structure -- composite material -- controlled study -- crystallization -- decomposition -- electron microscopy -- in vitro study -- in vivo study -- melting point -- mouse -- nonhuman -- ossification -- osteoblast -- physical chemistry -- priority journal -- rat -- strength -- structure analysis -- surface property -- synthesis -- temperature measurement -- thermal analysis -- tissue engineering -- wettability -- animal -- biomechanics -- bioremediation -- bone prosthesis -- cattle -- cell culture -- chemistry -- cytology -- differential scanning calorimetry -- drug effect -- human -- materials testing -- prostheses and orthoses -- scanning electron microscopy -- standard -- Wistar rat -- Murinae -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Biomechanics -- Bone Substitutes -- Cattle -- Cells, Cultured -- Differential Thermal Analysis -- Durapatite -- Humans -- Hydroxybutyrates -- Materials Testing -- Microscopy, Electron, Scanning -- Osteoblasts -- Prostheses and Implants -- Rats -- Rats, Wistar -- Surface Properties
Аннотация: Samples of a hybrid composite of polyhydroxybutyrate (PHB), a biodegradable polyester, and hydroxyapatite (HA), with different PHB/HA ratios, have been prepared using mechanical-physical method. Electron microscopy, X-ray structure analysis and differential thermal analysis have been used to investigate the structure and physicochemical properties of the composite, depending on the PHB/HA ratio. The properties of the surface of the HA-loaded composite are significantly different from those of the pure polymer. As the HA percentage in the composite increases, free interface energy, the cohesive force, i.e., the strength of the adhesive bond between the composite surface and the water phase, and surface wettability increase. The HA percentage of the composite does not influence its melting temperature, but affects the temperature for the onset of decomposition: as the HA content increases from 0 to 10% (w/w), Td decreases from 260В°C to 225В°C. The degree of crystallinity of PHB/HA increases from 77% to 89% with an increase in the HA fraction from 10% to 50%. Functional properties of the composites have been investigated in vitro and in vivo. The best parameters of growth and differentiation of murine marrow osteoblasts are registered on PHB/HA samples containing 10% and 20% HA. In ectopic bone formation assay it has been proven that the hybrid PHB/HA composites can function as scaffolds and that bone tissue develops on their surface and in pores. В© VSP 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Tomsk State University, Tomsk 634021, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Khlusov, I.A.; Volova, T.G.

Найти похожие
9.


   
    Results of biomedical investigations of PHB and PHB/PHV fibers / T. Volova [et al.] // Biochemical Engineering Journal. - 2003. - Vol. 16, Is. 2. - P125-133, DOI 10.1016/S1369-703X(03)00038-X . - ISSN 1369-703X
Кл.слова (ненормированные):
Biocompatibility -- Fibers -- Implants -- Poly-3-hydroxybutyrate (PHB) -- Poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV) -- Polyhydroxyalkanoates (PHAs) -- Tissue reaction -- Bacteriology -- Calcification (biochemistry) -- Copolymers -- Flammability -- Tissue -- Tissue response -- Biomedical engineering -- acid phosphatase -- poly(3 hydroxybutyric acid) -- poly(3 hydroxybutyric acid) co poly(3 hydroxyvaleric acid) -- polyhydroxyalkanoic acid -- suture material -- unclassified drug -- animal experiment -- article -- bacterial metabolism -- biochemistry -- biocompatibility -- biomedicine -- calcification -- catgut -- correlation analysis -- dose time effect relation -- enzyme activity -- fascia -- fiber -- foreign body -- functional assessment -- giant cell -- implantation -- in vivo study -- inflammation -- intermethod comparison -- macrophage -- materials testing -- multinuclear cell -- muscle injury -- necrosis -- nonhuman -- parameter -- phagocytosis -- physiology -- postoperative period -- priority journal -- process monitoring -- ralstonia eutropha b 5786 -- scar formation -- silk -- strength -- suture -- synthesis -- tissue reaction -- Wautersia eutropha -- wound healing -- Animalia -- Ralstonia -- Wautersia eutropha
Аннотация: The paper presents the results of biomedical investigations of sutures made of polyhydroxyalkanoates (PHAs) of two types (poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV)), synthesized by the bacteria Ralstonia eutropha B 5786 in the experiment on test animals in vivo, in comparison with silk and catgut sutures. PHB and PHB/PHV (PHBV) implants produced no adverse effect on physiological, biochemical and functional parameters of the animals during the post-surgery period. The tested PHA sutures featured the necessary strength throughout the healing period of the muscle-fascial cuts. The reaction of tissues to the implantation of PHB and PHB/PHV fibers fitted into the usual scheme characteristic of the wound process and of the reaction to a foreign-body invasion. This reaction and the reaction of tissues to silk had the similar nature and period of inflammation, but it was much less pronounced than the reaction to catgut. The tissue response to the implantation of PHAs consisted in a short-duration (up to 2 weeks) post-traumatic inflammation and the formation of a fibrous capsule less than 200 ?m thick during weeks 4-8, which in 4-6 months was reduced to 40-60 ?m in the course of reverse development. There were no adverse changes, such as suppurative inflammation, necrosis, calcification, and malignization of the cicatrice, at the site of implantation of PHA filaments, unlike in the cases with silk and catgut. In the case of PHA implantation there was a typical prolonged (throughout the post-surgery monitoring period) pronounced macrophagal stage with a large number of macrophages present. The macrophages were of the phagocytic type and multinucleate giant foreign cells, with a high activity of acid phosphomonoesterase that correlated with the activity of the enzyme in blood. Throughout the period of monitoring no differences in the tissue response to the implantation of the polymer filaments of two PHA types were recorded. В© 2003 Elsevier Science B.V. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 60036, Russian Federation
Inst. Transplantology Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Terr. Pathological Anatomy Bureau, Partisan Zheleznyak St. 1, Krasnoyarsk 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.; Shishatskaya, E.; Sevastianov, V.; Efremov, S.; Mogilnaya, O.

Найти похожие
 
Стандартный
Расширенный
Профессиональный
Распределенный
По словарю
ГРНТИ-навигатор
УДК-навигатор
Тематический навигатор

Другие библиотеки

Библиотека института физики
Центральная научная библиотека КНЦ СО РАН
Библиотека института химии и химический технологии
Библиотека института вычислительного моделирования
Библиотека института леса
Библиотека СФУ
Краевая научная библиотека
© Международная Ассоциация пользователей и разработчиков электронных библиотек и новых информационных технологий
(Ассоциация ЭБНИТ)