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Analysis of interactions between proteins and small-molecule drugs by a biosensor based on a graphene field-effect transistor / S. Xu, T. Wang, G. Liu [et al.] // Sens Actuators, B Chem. - 2021. - Vol. 326. - Ст. 128991, DOI 10.1016/j.snb.2020.128991 . - ISSN 0925-4005
Кл.слова (ненормированные):
Binding kinetics -- FET -- Imatinib -- LMW drugs -- Single-crystal graphene -- Biosensors -- Biosynthesis -- Drug interactions -- Graphene -- Graphene transistors -- Proteins -- Single crystals -- Graphene field-effect transistors -- Graphene sheets -- Interaction kinetics -- Linear response -- Low molecular weight drugs -- Real time -- Small-molecule drugs -- Target proteins -- Field effect transistors
Аннотация: We synthesized large-area single-crystal graphene sheets to use them in biosensors based on field-effect transistors (FET) for quantitative analysis of interaction kinetics and affinity between the imatinib drug and its target protein kinase Abl1. The G-FET biosensor showed an excellent performance and recognized imatinib at as low as 15.5 fM. The biosensor also showed a linear response to the logarithm of imatinib concentration in the 0.1 pM-10 ?M range. This graphene-based FET biosensor (G-FET) was also applied to quantify Abl1 Y253 F mutation and Abl1 dependency on Mg2+ to bind to imatinib in real-time. Results demonstrated in this work clearly showed that the novel G-FET biosensors are very promising to analyze interactions between proteins and low molecular weight drugs. © 2020 Elsevier B.V.

Scopus
Держатели документа:
Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation
Collaborative Innovation Center of Light Manipulations and Applications, Shandong Normal University, Jinan, 250358, China

Доп.точки доступа:
Xu, S.; Wang, T.; Liu, G.; Cao, Z.; Frank, L. A.; Jiang, S.; Zhang, C.; Li, Z.; Krasitskaya, V. V.; Li, Q.; Sha, Y.; Zhang, X.; Liu, H.; Wang, J.

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Synthesis and characterization of multicomponent PHAs / E. G. Kiselev, A. D. Vasiliev, T. G. Volova // J. Sib. Fed. Univ. - Biol. - 2021. - Vol. 14, Is. 1. - С. 97-113, DOI 10.17516/1997-1389-0325. - Cited By :1 . - ISSN 1997-1389
Кл.слова (ненормированные):
Biosynthesis -- Copolymers -- Physicochemical properties -- Polyhydroxyalkanoates (PHAs) -- Precursor substrates
Аннотация: Cupriavidus necator B10646 bacterial cells were cultivated in the mode of synthesis of the reserve polyhydroxyalkanoates (PHAs) in the growth medium that contained, in addition to glucose as the main substrate, precursor substrates of the monomers of various monocarboxylic acids - salts of valeric and hexanoic acids, propionate, and ?-butyrolactone. PHA terpolymers and quaterpolymers with different compositions and proportions of monomers were synthesized, and their physicochemical properties were studied. The terpolymers were composed of monomers of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), or 3-hydroxyhexanoate (3HHx) and had the following compositions: P(3HB/3HB/4HHx) and P(3HB/3HV/4HHx). The quaterpolymers had the following composition: P(3HB/3HV/4HB/3HHx). All copolymer samples, regardless of the composition and proportions of monomers, had lower molecular weights and higher polydispersity values compared to the highly crystalline 3-hydroxybutyrate homopolymer, but retained thermal stability properties, with a difference between the melting point and thermal degradation of at least 100-110 °C. The inclusion of 3HV, 4HB, and 3HHx monomers in the C-chain of 3HB caused changes in the crystalline to amorphous phase ratio and a significant decrease in the degree of crystallinity (Cx), which depended on the type of monomers and their contents in the copolymer. The maximum decrease in Cx (9-17 %) was detected in the P(3HB/3HV/4HB) terpolymer and the P(3HB/3HV/4HB/3HHx) quaterpolymer (30-36 %). The study confirms that there is the possibility of synthesizing polymers with various compositions, including new ones, which differ significantly in their basic properties. © Siberian Federal University. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
L.V. Kirenskii Institute of Physics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kiselev, E. G.; Vasiliev, A. D.; Volova, T. G.

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Biodegradable polymers - Perspectives and applications in agriculture / E. G. Kiselev, N. O. Zhila, T. G. Volova // IOP Conference Series: Earth and Environmental Science : IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст. 012036, DOI 10.1088/1755-1315/689/1/012036
Кл.слова (ненормированные):
Biodegradable polymers -- Ecosystems -- Fungi -- Glycerol -- Monounsaturated fatty acids -- Oilseeds -- Pesticides -- Substrates -- Sunflower oil -- Fenoxaprop-p-ethyl -- Natural materials -- Pesticide formulations -- Poly-3-hydroxybutyrate -- Polyhydroxyalkanoates -- Productive process -- Strategy of constructions -- Various substrates -- Palm oil
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.

Scopus
Держатели документа:
School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics, SB, RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kiselev, E. G.; Zhila, N. O.; Volova, T. G.

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Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by cupriavidus necator B-10646 from mixtures of oleic acid and 3-hydroxyvalerate precursors / N. O. Zhila, G. S. Kalacheva, V. V. Fokht [и др.] // J. Sib. Fed. Univ. - Biol. - 2020. - Vol. 13, Is. 3. - С. 331-341, DOI 10.17516/1997-1389-0320 . - ISSN 1997-1389
Кл.слова (ненормированные):
3-hydroxyvalerate -- Cupriavidus necator -- Fatty acids -- Molecular weight -- oleic acid
Аннотация: Polyhydroxyalkanoates have attracted much attention as biodegradable alternative to petroleum-based synthetic plastics. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] copolymer is one of the best characterized PHA copolymers because of its high commercial potential. However, commercial use of PHAs has been limited by their high price. One approach to reducing the cost of PHA production is to use inexpensive carbon sources (fatty acids, plant oils, etc.). The aim of this work was to study synthesis of P(3HB-co-3HV) by the Cupriavidus necator B-10646 bacterium grown on oleic acid and different biochemical precursors of 3HV. Bacterial cells were grown for 72 h at 30°C and 200 rpm on an incubator shaker. Salts of propionic or valeric acids were used as precursors of 3HV. The content and the composition of the polymer were determined by gas chromatography of fatty acid methyl esters. Lipids and polymer were extracted from biomass using the method of Folch. The addition of potassium propionate and valerate did not inhibit bacterial growth and polymer synthesis, the cell concentration and polymer content reaching 9.3-9.5 g/L and 80-83%, respectively. The addition of potassium valerate or propionate led to the synthesis of (P(3HB-co-3HV)) copolymer containing 21.2 and 14.3 mol% of 3HV, respectively. The number average molecular weight (Mn) of the polymer synthesized by the bacterium on oleic acid alone was 220 kDa; the polydispersity of the polymer was 3.5. The polymer synthesized in the presence of potassium valerate and propionate was characterized by a lower Mn (156-178 kDa) and a higher polydispersity of the polymer (4.4-4.9). The main fatty acids (FA) of intracellular lipids were oleic (33.26% of the total FA) and palmitic acid (27.48% of the total FA). The addition of potassium propionate or valerate did not cause any significant changes in the composition of the FA of intracellular lipids of the strain studied. This study demonstrates the ability of C. necator B-10646 to synthesize P(3HB-co-3HV) from mixtures of oleic acid and 3HV precursors. The data obtained can be used to develop and implement an economically feasible process of the P(3HB-co-3HV) production. © Siberian Federal University. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Zhila, N. O.; Kalacheva, G. S.; Fokht, V. V.; Bubnova, S. S.; Volova, T. G.

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Biophysics to Ecology / I. I. Gitel'zon // Her. Russ. Acad. Sci. - 2019. - Vol. 89, Is. 6. - P523-534, DOI 10.1134/S1019331619060066. - Cited References:25 . - ISSN 1019-3316. - ISSN 1555-6492

РУБ History & Philosophy Of Science + Multidisciplinary Sciences

Кл.слова (ненормированные):
biophysics -- biotechnology -- biosynthesis -- biopolymers -- bioluminescence -- bioluminescent analysis -- hydrogen biosynthesis -- noosphere -- life support -- closed ecosystem
Аннотация: This report presents some new methodological opportunities that biophysics can offer for solving the fundamental problem of planetary ecology-deciphering the mechanism that maintains the equilibrium state of the biosphere. The Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, where the author of this report works, is developing two complementary directions in ecological biophysics. The first is monitoring the vital activity of natural and constructed ecosystems by optical methods using the example of bioluminescence of the sea and closed ecosystems, and the second is studying the laws of parametric biosynthesis control and creating biotechnological control systems for these processes in order to construct noosphere-like ecosystems, in particular, for human life-support and survival systems in extreme conditions on the Earth and in space.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.
Russian Acad Sci, Krasnoyarsk, Russia.

Доп.точки доступа:
Gitel'zon, I. I.

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   Е071
   Б 63
Е07 / Б 63-ИБФ-КФ


    Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона [Текст] / И. И. Гительзон, Т. Г. Волова, А. Г. Дегерменджи [и др.] ; ред., авт. предисл. Т. Г. Волова ; Российская академия наук, Сибирское отделение, Институт биофизики (Красноярск). - Новосибирск : Издательство Сибирского отделения Российской академии наук, 2019. - 292, [2] с. : ил., цв. ил. ; 25 см. - Рез. ст. англ. - Библиогр. в конце ст. - 300 экз. - ISBN 978-5-7692-1650-3 : 1635.00 р.
    Содержание:
Гительзон, Иосиф Исаевич. Краткий очерк истории, состояния и перспектив = A short essay on the history, state and prospects of the institute of biophysics FRC KSC SB RAS / И. И. Гительзон. - С .14-23
Медведева, С. Е. Коллекция культур ибсо как база для исследований биолюминесценции й и грибов в ИБФ СО РАН = Culture collection ibso as a basis for research of bioluminescence of bacteria and fungi in IBP SB RAS / С. Е. Медведева. - С .24-39. - Библиогр.: с. 37-39
Гительзон, Иосиф Исаевич. Биолюминесценция Мирового океана = Bioluminescence of the World Ocean / И. И. Гительзон, Л. А. Левин, А. С. Артемкин, Р. Н., Чепилов В. В., Молвинских С.Л., Черепанов О. А., Чугунов Ю. В., Караев Н. Д., Загородний Ю. А., Шевырногов А. П. Утюшев Р. Н. - С .40-60. - Библиогр.: с. 60
Другие авторы: Левин Л. А., Артемкин А. С., Утюшев Р. Н., Чепилов В. В., Молвинских С.Л., Черепанов О. А., Чугунов Ю. В., Караев Н. Д., Загородний Ю. А., Шевырногов А. П.
Кратасюк, В. А. Бактериальная люцифераза в биолюминесцентном анализе = Bacterial luciferase in bioluminescent analysis / В. А. Кратасюк, Е. Н. Есимбекова. - С .61-71. - Библиогр.: с. 70-71
Франк, Л. А. Целентеразин-зависимые биолюминесцентные системы = Coelenterazine-dependent bioluminescent systems / Л. А. Франк. - С .72-87. - Библиогр.: с. 85-87
Кл.слова: люцифераза
Пуртов, К. В. Изучение химического механизма биолюминесценции грибов = The study of the chemical mechanism of bioluminescence of fungi / К. В. Пуртов, В. Н. Петушков, Н. С. Родионова. - С .88-98. - Библиогр.: с. 98
Родионова, Н. С. Исследование биолюминесценции сибирских почвенных олигохет = Study of siberian bioluminescent earthworms / Н. С. Родионова, А. А. Петушков. - С .99-118. - Библиогр.: с. 116-118
Тихомиров, А. А. Экспериментальные модели замкнутых экосистем с расчетной долей человека как перспективное направление исследований по созданию биолого-технической системы жизнеобеспечения = Experimental models of closed ecosystems with the human calculated limits as a perspective direction of research on the creation of BTLSS / А. А. Тихомиров, С. А. Ушакова, Н. А. Тихомирова, С. В., Величко В. В. Трифонов С. В. - С .119-128. - Библиогр.: с. 128
Другие авторы: Ушакова С. А., Тихомирова Н. А., Трифонов С. В., Величко В. В.
Волова, Татьяна Григорьевна. Управляемый биосинтез: от параметрически управляемых продуцирующих биосистем до новейших биофизических технологий = Controlled biosynthesis: from parametrically controlled producing biosystems to newest biophysical technologies / Т. Г. Волова, Е. И. Шишацкая. - С .129-148. - Библиогр.: с. 147-148
Бондарь, Владимир Станиславович. Биомедицинские приложения наноалмазов взрывного синтеза = Biomedical applications of nanodiamonds of explosive synthesis / В. С. Бондарь, А. П. Пузырь, Н. О. Ронжин, А. В., Буров А. Е. Барон А. В. - С .149-165. - Библиогр.: с. 161-165
Другие авторы: Пузырь А. П., Ронжин Н. О., Барон А. В., Буров А. Е.
Болсуновский, Александр Яковлевич. Применение радиоизотопных методов в институте биофизики СО РАН: от клеток крови до экосистем = Use od radioisotope techniques in the Institute of Biophysics SB RAS: from blood cells to ecosystems / А. Я. Болсуновский, С. В. Косиненко, Т. А. Зотина, Д. В. Дементьев. - С .166-179. - Библиогр.: с. 177-179
Другие авторы: Косиненко С. В., Зотина Т. А., Дементьев Д. В.
Шевырногов, Анатолий Петрович. Биосфера - взгляд сверху (экспрессные методы мониторинга биосферы в ИБФ СО РАН – ХХ–ХХI вв.) = biosphere - a view from space (express methods of the biosphere monitoring in the Institute of Biophysics SB RAS – XX–XXI century) / А. П. Шевырногов. - С .180-193. - Библиогр.: с. 193
Гладышев, Михаил Иванович. Жирные кислоты в экологической биофизике водных систем = Fatty acids in ecological biophysics of aquatic ecosystems / М. И. Гладышев. - С .194-209. - Библиогр.: с. 206-209
Рогозин, Денис Юрьевич. Сравнительное исследование устойчивости стратификации и структуры трофической сети в меромиктических озерах Шира и Шунет (Южная Сибирь, Россия) = Comparative study of the stability of stratification and the food web structure in the meromictic lakes Shira and Shunet (South Siberia, Russia) / Д. Ю. Рогозин, Е. С. Задереев, И. Г. Прокопкин [и др.]. - С .210-247. - Библиогр.: с. 243-247
Другие авторы: Задереев Е. С., Прокопкин И. Г., Толомеев А. П., Бархатов Ю. В., Хромечек Е. Б., Дегерменджи Н. Н., Дроботов А. В., Дегерменджи А. Г.
Печуркин, Николай Савельевич. Непрерывный рост интенсивности энерго-вещественных взаимодействий в эволюции геобиосферы Земли = Transparent growth of the energy/matter interactions on Earth in the evolution of geobiosphere / Н. С. Печуркин, А. Н. Шуваев, Л. А. Сомова. - С .248-254
Барцев, Сергей Иванович. Малоразмерные модели биосферы и феноменология изменения глобального климата = Small-scale biosphere models and phenomenology of global climate change / С. И. Барцев, А. Г. Дегерменджи. - С .255-283. - Библиогр.: с. 281-283
Дегерменджи, Андрей Георгиевич. Направления развития биофизики в Красноярске / А. Г. Дегерменджи. - С .284-288

ГРНТИ	31.27	76.03
УДК	577	574	61

ББК Е071я43 + Р252.0я43
Рубрики:
Экологическая биофизика
Медицинская биофизика
Кл.слова (ненормированные):
биолюминесценция -- люцифераза -- целентаразин -- олигохеты -- замкнутые экосистемы -- управляемый биосинтез -- наноалмазы -- радиоизотопные методы -- биосфера -- жирные кислоты -- системы жизнеобеспечения -- меромиктические озера -- геобиосфера -- эволюция -- глобальный климат -- Медицинская биофизика
Аннотация: Сборник посвящен широкому кругу исследований в области экологической биофизики – научного направления на стыке наук – от исследований на молекулярном уровне до вопросов управления большими природными экосистемами. Рассмотрены исторические вехи развития экологического направления биофизики. Основной акцент сборника основан на современных, актуальных достижениях красноярских биофизиков, которым удалось сохранить и развить многоплановые направления, которые были заложены в 50-х гг. ХХ века И. И. Гительзоном. Наряду с обзорными материалами и результатами фундаментальных исследований представлен ряд готовых к внедрению биотехнологий. Книга адресована биофизикам, экологам и химикам, а также преподавателям и студентам биофизических, биологических и экологических кафедр университетов.

Держатели документа:
Библиотека Института биофизики СО РАН : 660036, Академгородок, 50/12

Доп.точки доступа:
Гительзон, Иосиф Исаевич; Волова, Татьяна Григорьевна; Дегерменджи, Андрей Георгиевич; Дегерменджи, Н. Н.; Шевырногов, Анатолий Петрович; Кратасюк, В. А.; Барцев, Сергей иванович; Болсуновский, Александр Яковлевич; Бондарь, Владимир Антонович; Буров, А. Е.; Величко, В. В.; Гладышев, Михаил Иванович; Есимбекова, Е. Н.; Дементьев, Д. В.; Задереев, Егор Сергеевич; Зотина, Т. А.; Косиненко, Сергей Васильевич; Медведева, С. Е.; Петушков, В. Н.; Печуркин, Николай Савельевич; Прокопкин, И. Г.; Пузырь, А. П.; Пуртов, К. В.; Рогозин, Денис Юрьевич; Родионова, Н. С.; Ронжин, Н. О.; Сомова, Лидия Александровна; Тихомиров, Александр Аполлинариевич; Тихомирова, Наталья Александровна; Трифонов, С. В.; Ушакова, Софья Аврумовна; Франк, Л. А.; Хромечек, Е. Б.; Шишацкая, Е. И.; Шуваев, А. Н.; Волова, Татьяна Григорьевна \ред., авт. предисл.\; Утюшев Р. Н., Чепилов В. В., Молвинских С.Л., Черепанов О. А., Чугунов Ю. В., Караев Н. Д., Загородний Ю. А., Шевырногов А. П.; Трифонов С. В., Величко В. В.; Барон А. В., Буров А. Е.; Толомеев А. П., Бархатов Ю. В., Хромечек Е. Б., Дегерменджи Н. Н.; Дроботов А. В.; Дегерменджи А. Г., Андрей Георгиевич; Гительзон, Иосиф Исаевич \о нем\; Российская академия наук. Сибирское отделение; Институт биофизики (Красноярск)
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The effect of the chemical composition and structure of polymer films made from resorbable polyhydroxyalkanoates on blood cell response / E. I. Shishatskaya, N. G. Menzyanova, A. A. Shumilova // Int. J. Biol. Macromol. - 2019. - Vol. 141. - P765-+, DOI 10.1016/j.ijbiomac.2019.09.015. - Cited References:57. - The authors disclosed receipt of the following financial support for the research, authorship, and/or publication of this article: The study is supporting by the Russian Science Foundation, Project No 17-15-01352. . - ISSN 0141-8130. - ISSN 1879-0003

РУБ Biochemistry & Molecular Biology + Chemistry, Applied + Polymer Science
Рубрики:
3-HYDROXYBUTYRATE
   BIOCOMPATIBILITY
   BIOSYNTHESIS
   ADHESION
   STENTS
Кл.слова (ненормированные):
Degradable polyhydroxyalkanoates (PHAs) -- Surface structure and -- properties -- Blood cell response
Аннотация: Four PHA types were synthesized in the culture of Cupriavidus eutrophus B-10646 under special conditions, poly(3 hydroxybutyrate) [P(3HB)] and of copolymers, which contained 3HB monomers and 4 hydroxybutyrate (4HB), 3 hydroxyvalerate (3HV), or 3 hydroxyhexanoate (3HHx). All copolymers had the M-w of about 550-670 kDa, and the homopolymer P(3HB) had a significantly higher M-w - 920 kDa. P(3HB co 4HB) and P(3HB co 3HHx) had the lowest C-x (42 and 49%) while P(3HB co 3HV) and P(3HB) exhibited higher C-x values (76%). Polymer films were prepared from different PHAs. Electron microscopy showed differences in the surface microstructure of the films. Films prepared from the P(3HB) were more hydrophobic and the arithmetic mean surface roughness of 71-75 nm, than the copolymer films, which were hydrophilic (57-60 degrees) and had considerably higher roughness (158-177 nm). Blood parameters (hemoglobin and hemolysis) and response of the cells (erythrocytes, platelets, and monocytes) were studied in experiments with blood directly contacting the surface of the films of PHAs with different compositions. Cultivation of blood cells on polymer films did not cause any adverse effects on adhesion and morphology of all cell types. Results of studying blood cell response suggested that the films made from low-crystallinity copolymers containing 4 hydroxybutyrate and 3 hydroxyhexanoate were the best for contact with blood. (C) 2019 Elsevier B.V. All rights reserved.

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Держатели документа:
Siberian Fed Univ, 79 Svobodnyi Ave, Krasnoyarsk 660041, Russia.
RAS, SB, Krasnoyarsk Sci Ctr, Inst Biophys,Fed Res Ctr, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Shishatskaya, Ekaterina I.; Menzyanova, Natalia G.; Shumilova, Anna A.; Russian Science FoundationRussian Science Foundation (RSF) [17-15-01352]

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Biophysics to Ecology / I. I. Gitel’zon // Her. Russ. Acad. Sci. - 2019. - Vol. 89, Is. 6. - P523-534, DOI 10.1134/S1019331619060066 . - ISSN 1019-3316
Кл.слова (ненормированные):
bioluminescence -- bioluminescent analysis -- biophysics -- biopolymers -- biosynthesis -- biotechnology -- closed ecosystem -- hydrogen biosynthesis -- life support -- noosphere
Аннотация: Abstract: This report presents some new methodological opportunities that biophysics can offer for solving the fundamental problem of planetary ecology—deciphering the mechanism that maintains the equilibrium state of the biosphere. The Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, where the author of this report works, is developing two complementary directions in ecological biophysics. The first is monitoring the vital activity of natural and constructed ecosystems by optical methods using the example of bioluminescence of the sea and closed ecosystems, and the second is studying the laws of parametric biosynthesis control and creating biotechnological control systems for these processes in order to construct noosphere-like ecosystems, in particular, for human life-support and survival systems in extreme conditions on the Earth and in space. © 2019, Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Gitel’zon, I. I.

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   Е071
   Б 63

    Волова, Татьяна Григорьевна.
    Управляемый биосинтез: от параметрически управляемых продуцирующих биосистем до новейших биофизических технологий [Текст] = Controlled biosynthesis: from parametrically controlled producing biosystems to newest biophysical technologies / Т. Г. Волова, Е. И. Шишацкая, Величко В. В. Трифонов С. В. // Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона / И. И. Гительзон, Т. Г. Волова, А. Г. Дегерменджи [и др.] ; ред., авт. предисл. Т. Г. Волова. - Новосибирск : Издательство Сибирского отделения Российской академии наук, 2019. - С. 129-148. - Библиогр.: с. 147-148 . - ISBN 978-5-7692-1650-3

УДК

577

574

ББК Е071я43 + Р252.0я43

Доп.точки доступа:
Гительзон, Иосиф Исаевич; Дегерменджи, Андрей Георгиевич; Дегерменджи, Н. Н.; Шевырногов, Анатолий Петрович; Кратасюк, В. А.; Барцев, Сергей иванович; Болсуновский, Александр Яковлевич; Бондарь, Владимир Антонович; Буров, А. Е.; Величко, В. В.; Гладышев, Михаил Иванович; Есимбекова, Е. Н.; Дементьев, Д. В.; Задереев, Егор Сергеевич; Зотина, Т. А.; Косиненко, Сергей Васильевич; Медведева, С. Е.; Петушков, В. Н.; Печуркин, Николай Савельевич; Прокопкин, И. Г.; Пузырь, А. П.; Пуртов, К. В.; Рогозин, Денис Юрьевич; Родионова, Н. С.; Ронжин, Н. О.; Сомова, Лидия Александровна; Тихомиров, Александр Аполлинариевич; Тихомирова, Наталья Александровна; Трифонов, С. В.; Ушакова, Софья Аврумовна; Франк, Л. А.; Хромечек, Е. Б.; Шишацкая, Е. И.; Шуваев, А. Н.; Шишацкая, Екатерина Игоревна; Трифонов С. В., Величко В. В.; Российская академия наук. Сибирское отделение; Институт биофизики(Красноярск)

Имеются экземпляры в отделах: всего 1 : ИБФ-КФ (1)
Свободны: ИБФ-КФ (1)

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10.

Genetically encodable bioluminescent system from fungi / A. A. Kotlobay [et al.] // Proc. Natl. Acad. Sci. U. S. A. - 2018. - Vol. 115, Is. 50. - P12728-12732, DOI 10.1073/pnas.1803615115 . - ISSN 0027-8424
Кл.слова (ненормированные):
Bioluminescence -- Fungal luciferase -- Fungal luciferin biosynthesis
Аннотация: Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually oriented functions from attracting mates to scaring off predators. Half a dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which is found only in bacteria. Here, we report identification of the fungal luciferase and three other key enzymes that together form the biosynthetic cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite. Introduction of the identified genes into the genome of the yeast Pichia pastoris along with caffeic acid biosynthesis genes resulted in a strain that is autoluminescent in standard media. We analyzed evolution of the enzymes of the luciferin biosynthesis cycle and found that fungal bioluminescence emerged through a series of events that included two independent gene duplications. The retention of the duplicated enzymes of the luciferin pathway in nonluminescent fungi shows that the gene duplication was followed by functional sequence divergence of enzymes of at least one gene in the biosynthetic pathway and suggests that the evolution of fungal bioluminescence proceeded through several closely related stepping stone nonluminescent biochemical reactions with adaptive roles. The availability of a complete eukaryotic luciferin biosynthesis pathway provides several applications in biomedicine and bioengineering. © 2018 National Academy of Sciences. All Rights Reserved.

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Держатели документа:
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russian Federation
Planta LLC, Moscow, 121205, Russian Federation
Institute of Science and Technology Austria, Klosterneuburg, 3400, Austria
Medical Research Council London Institute of Medical Sciences, Imperial College London, London, W12 0NN, United Kingdom
Centre for Genomic Regulation, Barcelona Institute for Science and Technology, Barcelona, 08003, Spain
Universitat Pompeu Fabra, Barcelona, 08003, Spain
Evrogen JSC, Moscow, 117997, Russian Federation
Institute of Biophysics, Federal Research Center Krasnoyarsk Science Center, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Moscow, 142290, Russian Federation
Pirogov Russian National Research Medical University, Moscow, 117997, Russian Federation
Biomedical Nanomaterials, National Research Technological University (MISiS), Moscow, 119049, Russian Federation
Skolkovo Institute of Science and Technology, Moscow, 121205, Russian Federation
Departamento de Bioquimica, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, 05508-000, Brazil
Departamento de Oceanografia Fisica, Quimica e Geologica, Instituto Oceanografico, Universidade de Sao Paulo, Sao Paulo, 05508-120, Brazil
Department of Environmental Biology, Chubu University, Kasugai, 487-8501, Japan
Catalan Institution for Research and Advanced Studies (ICREA), Barcelona, 08010, Spain
Departamento de Quimica Fundamental, Instituto de Quimica, Universidade de Sao Paulo, Sao Paulo, 05508-000, Brazil

Доп.точки доступа:
Kotlobay, A. A.; Sarkisyan, K. S.; Mokrushina, Y. A.; Marcet-Houben, M.; Serebrovskaya, E. O.; Markina, N. M.; Somermeyer, L. G.; Gorokhovatsky, A. Y.; Vvedensky, A.; Purtov, K. V.; Petushkov, V. N.; Rodionova, N. S.; Chepurnyh, T. V.; Fakhranurova, L. I.; Guglya, E. B.; Ziganshin, R.; Tsarkova, A. S.; Kaskova, Z. M.; Shender, V.; Abakumov, M.; Abakumova, T. O.; Povolotskaya, I. S.; Eroshkin, F. M.; Zaraisky, A. G.; Mishin, A. S.; Dolgov, S. V.; Mitiouchkina, T. Y.; Kopantzev, E. P.; Waldenmaier, H. E.; Oliveira, A. G.; Oba, Y.; Barsova, E.; Bogdanova, E. A.; Gabaldon, T.; Stevani, C. V.; Lukyanov, S.; Smirnov, I. V.; Gitelson, J. I.; Kondrashov, F. A.; Yampolsky, I. V.

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11.

Biosynthesis and properties of P(3HB-co-3HV-co-3H4MV) produced by using the wild-type strain Cupriavidus eutrophus B-10646 / T. Volova [et al.] // Journal of Chemical Technology and Biotechnology. - 2018, DOI 10.1002/jctb.5763 . - Article in press. - ISSN 0268-2575
Кл.слова (ненормированные):
3-hydroxy-4-methylvalerate -- physicochemical properties -- polyhydroxyalkanoates -- synthesis -- Biochemistry -- Chemical industry -- Synthesis (chemical) -- 3-hydroxy-4-methylvalerate -- Crystalline-to-amorphous -- Degree of crystallinity -- Degrees of crystallinity -- Physicochemical property -- Polyhydroxyalkanoates -- Processing properties -- Wild-type strain -- Polymers
Аннотация: BACKGROUND: Polyhydroxyalkanoates (PHA) containing 3-hydroxy-4-methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3 HV-co-3H4MV)] by Cupriavidus eutrophus B-10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS: It was shown that 4-methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L?1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION: The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry

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Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
L.V. Kirenskii Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
International and Inter University Centre of Nanoscience and Nanotechnology, Mahatam Gandhi University, Kottyam, India

Доп.точки доступа:
Volova, T.; Menshikova, O.; Zhila, N.; Vasiliev, A.; Kiselev, E.; Peterson, I.; Shishatskaya, E.; Thomas, S.

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12.

Natural-based polymers for biomedical applications / T. G. Volova [et al.] // : Apple Academic Press, 2017. - P1-439, DOI 10.1201/9781315366036
Аннотация: This new book presents the authors’ biomedical studies of natural degradable biopolymers (polyhydroxyalkanoates [PHAs]) and discusses the demand for medical-grade materials and modern trends, focusing on the present status and future potential of PHAs. The authors present and summarize their most important results and findings obtained during the last few years in experimental studies and clinical trials of PHAs at the Institute of Biophysics Siberian Branch of Russian Academy of Science. © 2017 by Apple Academic Press, Inc.

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Держатели документа:
Department of Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Russian Federation
Department of General Surgery, Krasnoyarsk State Medical School, Krasnoyarsk, Russian Federation
Department of Medical Biology, Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Polymer Division, N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russian Federation
Moscow State Academy of Fine Chemical Technology, Russian Federation
Kazan National Research Technological University, Kazan, Russian Federation

Доп.точки доступа:
Volova, T. G.; Vinnik, Y. S.; Shishatskaya, E. I.; Markelova, N. M.; Zaikov, G. E.

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13.

Fungal bioluminescence system: luciferin, luciferase and luciferin biosynthesis / I. Yampolsky // FEBS J. - 2017. - Vol. 284: 42nd Congress of the Federation-of-European-Biochemical-Societies (FEBS) (SEP 10-14, 2017, Jerusalem, ISRAEL). - P189-189. - Cited References:0. - This work was supported by the Russian Science Foundation grant 16-14-00052. . - ISSN 1742-464X. - ISSN 1742-4658

РУБ Biochemistry & Molecular Biology

WOS
Держатели документа:
Russian Acad Sci, Inst Bioorgan Chem, Moscow, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.
Доп.точки доступа:
Yampolsky, I.; Russian Science Foundation [16-14-00052]

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14.

A study of synthesis and properties of poly-3-hydroxybutyrate/diethylene glycol copolymers / T. Volova [et al.] // Biotechnol. Prog. - 2016. - Vol. 32, Is. 4. - P1017-1028, DOI 10.1002/btpr.2267 . - ISSN 8756-7938
Кл.слова (ненормированные):
biocompatibility -- biosynthesis -- molecular weight -- physicochemical and mechanical properties -- poly(3-hydroxybutyrate)/diethylene glycol copolymers -- Biochemistry -- Biocompatibility -- Biosynthesis -- Cell culture -- Cell membranes -- Cells -- Cytology -- Fatty acids -- Glycols -- Molecular weight -- American Institute of Chemical Engineers -- Biological properties -- Chemical compositions -- Cytoplasmic membrane -- Degree of saturations -- Physico-chemical and mechanical properties -- Physiological effects -- Poly-3-hydroxybutyrate -- Biomechanics
Аннотация: This study investigates synthesis of poly(3-hydroxybutyrate)/diethylene glycol copolymers (P3HB/DEG) by Cupriavidus eutrophus B-10646 cells as related to DEG concentration in the medium and the time when it is added to the culture of cells synthesizing P3HB. The study determines the limits of physiological effect of DEG on C. eutrophus cells, showing that at DEG concentrations above 30 g/L, it inhibits cell growth, decreasing cell concentration and total P3HB/DEG yield and inducing an increase in the degree of saturation of fatty acids in lipids of cell cytoplasmic membrane. A series of copolymers containing different molar fractions of DEG (between 0.13 and 3.0 mol%) have been synthesized and their physicochemical, physical/mechanical, and biological properties have been investigated as related to the chemical composition and proportions of DEG monomers of the polymers. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1017–1028, 2016. © 2016 American Institute of Chemical Engineers

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Держатели документа:
Inst. of Biophysics SB RAS, Akademgorodok 50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T.; Zhila, N.; Kiselev, E.; Shishatskaya, E.

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15.

Evolution and present status of experimental manned ecological systems for long-term human life support - Bios, developed by the institute of biophysics of Russian academy of sciences in Krasnoyarsk (Siberia) / J. I. Gitelson, A. G. Degermendzhy // Proceedings of the International Astronautical Congress, IAC : International Astronautical Federation, IAF, 2015. - Vol. 1: 66th International Astronautical Congress 2015: Space - The Gateway for Mankind's Future, IAC 2015 (12 October 2015 through 16 October 2015, ) Conference code: 122921. - P243-250
Кл.слова (ненормированные):
Arid regions -- Biochemistry -- Biospherics -- Plant shutdowns -- Proteins -- Reconfigurable hardware -- Closed ecological systems -- Corrective actions -- Essential proteins -- Extreme conditions -- Human intelligence -- Long-term experiments -- Physiological effects -- Russian Academy of Sciences -- Ecology
Аннотация: Closed ecological systems are of two-fold interest - as models of the Earth's biosphere explorable in experiments and as a facility for long-term autonomous human life support beyond the Earth. Theoretical analysis and experimental implementation of highly closed manned systems has been the subject of studies at the Institute of Biophysics (Russian Academy of Sciences, Siberian Branch) for many years. BIOS systems of increasing complexity with complete regeneration of atmosphere, water and partially food have been realized. In BIOS-3 experiments the system inhabited by 2-3 researchers for 4-6 months maintained its metabolic equilibrium without any negative physiological effect on the crew, which proves its sustainable condition. Specific for BIOS-3 is internal control by the people inhabiting the system. So, BIOS-3 is the first experimental implementation of V.l. Vernadsky's idea about the noosphere - habitable Biosphere controlled by human intelligence. Contrary to predictions of many environmentalists the closedness of the ecosystem is a factor that does not reduce, but increases its sustainability and makes its use for reliable life support outside the Earth realistic. The system is sustainable owing to permanent feedback between the monitoring of few key parameters of the system and automatic corrective actions on them. Main object of control is photo-biosynthesis regenerating parameters of human habitat disturbed by his vital activities. This principle has been realized in BIOS system and proved its reliability in long-term experiments. A new challenge is specified-optimal increase of trophic closedness of the system by reproduction within it essential proteins (peptides and amino acids), lipids, vitamins and other essential compounds. Alternative lines of attack on this problem by state-of-the-art biotechnological methods, GMO including, are under analysis. Reduced BIOS version - without complete closure - can be a breakthrough instrument to improve the quality of life of people living under extreme conditions on the Earth - in polar latitudes (Arctic, Antarctic), in deserts, in high mountains.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Gitelson, J. I.; Degermendzhy, A. G.

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16.

The Chemical Basis of Fungal Bioluminescence / K. V. Purtov [et al.] // Angew. Chem. Int. Ed. - 2015. - Vol. 54, Is. 28. - P8124-8128, DOI 10.1002/anie.201501779 . - ISSN 1433-7851
Кл.слова (ненормированные):
bioluminescence -- bioorganic chemistry -- biosynthesis -- luciferin -- natural products -- Biochemistry -- Bioluminescence -- Biosynthesis -- Metabolites -- Phosphorescence -- Biochemical mechanisms -- Bioorganic chemistry -- luciferin -- Natural products -- Plant secondary metabolites -- Structural similarity -- Fungi
Аннотация: Many species of fungi naturally produce light, a phenomenon known as bioluminescence, however, the fungal substrates used in the chemical reactions that produce light have not been reported. We identified the fungal compound luciferin 3-hydroxyhispidin, which is biosynthesized by oxidation of the precursor hispidin, a known fungal and plant secondary metabolite. The fungal luciferin does not share structural similarity with the other eight known luciferins. Furthermore, it was shown that 3-hydroxyhispidin leads to bioluminescence in extracts from four diverse genera of luminous fungi, thus suggesting a common biochemical mechanism for fungal bioluminescence. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow, Russian Federation
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow, Russian Federation

Доп.точки доступа:
Purtov, K.V.; Petushkov, V.N.; Baranov, M.S.; Mineev, K.S.; Rodionova, N.S.; Kaskova, Z.M.; Tsarkova, A.S.; Petunin, A.I.; Bondar, V.S.; Rodicheva, E.K.; Medvedeva, S.E.; Oba, Y.; Arseniev, A.S.; Lukyanov, S.; Gitelson, J.I.; Yampolsky, I.V.

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17.

AsLn2, a luciferin-related modified tripeptide from the bioluminescent earthworm Fridericia heliota / V. N. Petushkov [et al.] // Tetrahedron Letters. - 2014. - Vol. 55, Is. 2. - P463-465, DOI 10.1016/j.tetlet.2013.11.061 . - ISSN 0040-4039
Кл.слова (ненормированные):
Bioluminescence -- Fridericia heliota -- Luciferin -- Modified peptide
Аннотация: AsLn2, an unusual modified peptide, was isolated from the bioluminescent earthworm Fridericia heliota (Enchytraeidae). Its structure, elucidated by NMR and mass spectrometry, includes residues of tyrosine, CompX (a novel tyrosine modification product, reported in the accompanying paper), and N(omega)-acylated lysine. Chromatography, UV, and 1H NMR data imply a close structural similarity of AsLn2 with F. heliota luciferin. AsLn2 appears to be an intermediate or by-product in F. heliota luciferin biosynthesis. © 2013 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Laboratory of Bioluminescent Biotechnologies, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, pr. Svobodnyi, 79, Krasnoyarsk 660041, Russian Federation
Laboratory of Photobiology, Institute of Biophysics, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117997, Russian Federation
Department of Chemistry and Biochemistry, Faculty of Sciences, University of Porto, Campo Alegre St. 687, Porto 4169-007, Portugal
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Dubinnyi, M.A.; Rodionova, N.S.; Nadezhdin, K.D.; Marques, S.M.; Esteves Da Silva, J.C.G.; Shimomura, O.; Yampolsky, I.V.

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18.

A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs [Text] / T. . Volova [et al.] // PLoS One. - 2014. - Vol. 9, Is. 2. - Ст. e87551, DOI 10.1371/journal.pone.0087551. - Cited References: 64. - This study was financially supported by Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" (Agreement No. 11.G34.31.0013 with Amendment No. 1 of 15 February 2013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. . - ISSN 1932-6203

РУБ Multidisciplinary Sciences
Рубрики:
RALSTONIA-EUTROPHA
   BIODEGRADABLE POLYHYDROXYALKANOATES
   AEROMONAS-HYDROPHILA
   ESCHERICHIA-COLI
   MOLECULAR-WEIGHT
   SURFACE-ENERGY
   NORTH PACIFIC
   TERPOLYESTER
   BIOSYNTHESIS
   POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE-CO-3-HYDROXYHEXANOATE)
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.

WOS
Держатели документа:
[Volova, Tatiana
Kiselev, Evgeniy
Nikolaeva, Elena
Sukovatiy, Aleksey
Shishatskaya, Ekaterina] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
[Volova, Tatiana
Vinogradova, Olga
Shishatskaya, Ekaterina] Siberian Fed Univ, Krasnoyarsk, Russia
[Chistyakov, Anton] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T...; Kiselev, E...; Vinogradova, O...; Nikolaeva, E...; Chistyakov, A...; Sukovatiy, A...; Shishatskaya, E...; Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" [11.G34.31.0013]

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19.

Comparative study of Thermoresistances' cellular mechanisms in representatives of the Gammarus lacustris Sars populations inhabiting Saline Lake Shira (Republic of Khakassia) and a fresh water body in the Cis-Baikal region / Z. M. Shatilina [et al.] // Doklady Biological Sciences. - 2010. - Vol. 434, Is. 1. - P359-362, DOI 10.1134/S0012496610050182 . - ISSN 0012-4966
Кл.слова (ненормированные):
antioxidant -- catalase -- fresh water -- heat shock protein 70 -- reactive oxygen metabolite -- sea water -- Amphipoda -- animal -- article -- biosynthesis -- comparative study -- ecosystem -- heat -- metabolism -- Russian Federation -- Amphipoda -- Animals -- Antioxidants -- Catalase -- Ecosystem -- Fresh Water -- Hot Temperature -- HSP70 Heat-Shock Proteins -- Reactive Oxygen Species -- Seawater -- Siberia -- Gammarus -- Gammarus lacustris

Scopus
Держатели документа:
Institute of Biology, Irkutsk State University, ul. Lenina 3, Irkutsk 664003, Russian Federation
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, pr. Svobodnyi 79, Krasnoyarsk 660041, Russian Federation
Irkutsk State University, ul. Karla Marksa 1, Irkutsk 664003, Russian Federation
Baikal Research Centre, ul. Karla Marksa 5-10, Irkutsk 664003, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shatilina, Z.M.; Gubanov, M.V.; Zadereev, E.S.; Pavlichenko, V.V.; Axenov-Gribanov, D.V.; Sapozhnikova, E.A.; Protopopova, M.V.; Bedulina, D.S.; Timofeyev, M.A.

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20.

Synthesis of reserve polyhydroxyalkanoates by luminescent bacteria / A. N. Boyandin [et al.] // Microbiology. - 2008. - Vol. 77, Is. 3. - P318-323, DOI 10.1134/S0026261708030119 . - ISSN 0026-2617
Кл.слова (ненормированные):
Biosynthesis -- Chemical structure -- Luminescent bacteria -- Polyhydroxyalkanoates (PHA) -- Bacteria (microorganisms) -- Photobacterium leiognathi -- Vibrio harveyi
Аннотация: The ability of marine luminescent bacteria to synthesize polyesters of hydroxycarboxylic acids (polyhydroxyalkanoates, PHA) as reserve macromolecules was studied. Twenty strains from the collection of the luminescent bacteria CCIBSO (WDCM839) of the Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, assigned to different taxa (Photobacterium leiognathi, Ph. phosphoreum, Vibrio harveyi, and V. fischeri) were analyzed. The most productive strains were identified, and the conditions ensuring high polymer yields in batch culture (40-70% of the cell dry mass weight) were determined. The capacity for synthesizing two-and three-component polymers containing hydroxybutyric acid as the main monomer and hydroxyvaleric and hydroxyhexanoic acids was revealed in Ph. leiognathi and V. harveyi strains. The results allow luminescent microorganisms to be regarded as new producers of multicomponent polyhydroxyalkanoates. В© 2008 MAIK Nauka.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.N.; Kalacheva, G.S.; Rodicheva, E.K.; Volova, T.G.

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