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Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786 [Text] / T. G. Volova [et al.] // Appl. Biochem. Microbiol. - 2004. - Vol. 40, Is. 2. - P. 170-177, DOI 10.1023/B:ABIM.0000018921.04863.d5. - Cited References: 27 . - ISSN 0003-6838

РУБ Biotechnology & Applied Microbiology + Microbiology
Рубрики:
POLY-BETA-HYDROXYBUTYRATE
   ORGANISM ALCALIGENES-EUTROPHUS
   ESCHERICHIA-COLI
   PHB
   POLY(3-HYDROXYBUTYRATE)
   BIOSYNTHESIS
   CLONING
   GENES
   CHAIN
   H16
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (beta-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of beta-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Gorbunova, O.V.; Zhila, N.O.

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Biosynthesis of tetrahydrofolate in plants: Crystal structure of 7,8-dihydroneopterin aldolase from Arabidopsis thaliana reveals a novel adolase class [Text] / S. . Bauer [et al.] // J. Mol. Biol. - 2004. - Vol. 339, Is. 4. - P. 967-979, DOI 10.1016/j.jmb.2004.04.034. - Cited References: 66 . - ISSN 0022-2836

РУБ Biochemistry & Molecular Biology
Рубрики:
GTP CYCLOHYDROLASE-I
   GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE
   6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE
   ESCHERICHIA-COLI
   DIHYDRONEOPTERIN ALDOLASE
   FOLIC-ACID
   ENZYMATIC SYNTHESIS
   DIHYDROPTEROATE SYNTHASE
   REACTION-MECHANISM
   3-DIMENSIONAL STRUCTURE
Кл.слова (ненормированные):
tetrahydrofolate biosynthesis -- aldolase classes -- retroaldol reaction -- purin binding -- Schiff base
Аннотация: Dihydroneopterin aldolase (DHNA) catalyses a retroaldol reaction yielding 6-hydroxymethyl-7,8-dihydropterin, a biosynthetic precursor of the vitamin, tetrahydrofolate. The enzyme is a potential target for antimicrobial and anti-parasite chemotherapy. A gene specifying a dihydroneopterin aldolase from Arabidopsis thaliana was expressed in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity and crystallised using polyethylenglycol as the precipitating agent. The crystal structure was solved by X-ray diffraction analysis at 2.2 Angstrom resolution. The enzyme forms a D-4-symmetric homo-octamer. Each polypeptide chain is folded into a single domain comprising an antiparallel four-stranded beta-sheet and two long alpha-helices. Four monomers are arranged in a tetrameric ring, and two of these rings form a hollow cylinder. Well defined purine derivatives are found at all eight topologically equivalent active sites. The subunit fold of the enzyme is related to substructures of dihydroneopterin triphosphate epimerase, GTP cyclohydrolase I, and pyruvoyltetrahydropterin synthase, which are all involved in the biosynthesis of pteridine type cofactors, and to urate oxidase, although some members of that superfamily have no detectable sequence similarity Due to structural and mechanistical differences of DHNA in comparison with class I and class II aldolases, a new aldolase class is proposed. (C) 2004 Elsevier Ltd. All rights reserved.

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Держатели документа:
Max Planck Inst Biochem, Abt Strukturforsch, D-82152 Martinsried, Germany
Tech Univ Munich, Lehrstuhl Organ Chem & Biochem, D-85747 Garching, Germany
Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Bauer, S...; Schott, A.K.; Illarionova, V...; Bacher, A...; Huber, R...; Fischer, M...

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Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha grown under conditions favoring polyhydroxybutyrate biosynthesis [Text] / T. G. Volova, N. A. Voinov // Appl. Biochem. Microbiol. - 2003. - Vol. 39, Is. 2. - P. 166-170, DOI 10.1023/A:1022585912873. - Cited References: 15 . - ISSN 0003-6838

РУБ Biotechnology & Applied Microbiology + Microbiology
Рубрики:
ALCALIGENES
Аннотация: Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha grown on a gas substrate under conditions favoring autotrophic biosynthesis of polyhydroxybutyrate were studied. The following parameters, making it possible to control and optimize the process in industrial situations, were determined: specific rate of substrate consumption, physical properties of the culture medium, and coefficients of heat emission and mass transfer.

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Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660033, Russia
Siberian State Technol Univ, Minist Educ Russian Federat, Krasnoyarsk 660049, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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Evolution and present status of experimental manned ecological systems for long-term human life support - Bios, developed by the institute of biophysics of Russian academy of sciences in Krasnoyarsk (Siberia) / J. I. Gitelson, A. G. Degermendzhy // Proceedings of the International Astronautical Congress, IAC : International Astronautical Federation, IAF, 2015. - Vol. 1: 66th International Astronautical Congress 2015: Space - The Gateway for Mankind's Future, IAC 2015 (12 October 2015 through 16 October 2015, ) Conference code: 122921. - P243-250
Кл.слова (ненормированные):
Arid regions -- Biochemistry -- Biospherics -- Plant shutdowns -- Proteins -- Reconfigurable hardware -- Closed ecological systems -- Corrective actions -- Essential proteins -- Extreme conditions -- Human intelligence -- Long-term experiments -- Physiological effects -- Russian Academy of Sciences -- Ecology
Аннотация: Closed ecological systems are of two-fold interest - as models of the Earth's biosphere explorable in experiments and as a facility for long-term autonomous human life support beyond the Earth. Theoretical analysis and experimental implementation of highly closed manned systems has been the subject of studies at the Institute of Biophysics (Russian Academy of Sciences, Siberian Branch) for many years. BIOS systems of increasing complexity with complete regeneration of atmosphere, water and partially food have been realized. In BIOS-3 experiments the system inhabited by 2-3 researchers for 4-6 months maintained its metabolic equilibrium without any negative physiological effect on the crew, which proves its sustainable condition. Specific for BIOS-3 is internal control by the people inhabiting the system. So, BIOS-3 is the first experimental implementation of V.l. Vernadsky's idea about the noosphere - habitable Biosphere controlled by human intelligence. Contrary to predictions of many environmentalists the closedness of the ecosystem is a factor that does not reduce, but increases its sustainability and makes its use for reliable life support outside the Earth realistic. The system is sustainable owing to permanent feedback between the monitoring of few key parameters of the system and automatic corrective actions on them. Main object of control is photo-biosynthesis regenerating parameters of human habitat disturbed by his vital activities. This principle has been realized in BIOS system and proved its reliability in long-term experiments. A new challenge is specified-optimal increase of trophic closedness of the system by reproduction within it essential proteins (peptides and amino acids), lipids, vitamins and other essential compounds. Alternative lines of attack on this problem by state-of-the-art biotechnological methods, GMO including, are under analysis. Reduced BIOS version - without complete closure - can be a breakthrough instrument to improve the quality of life of people living under extreme conditions on the Earth - in polar latitudes (Arctic, Antarctic), in deserts, in high mountains.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Gitelson, J. I.; Degermendzhy, A. G.

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A study of synthesis and properties of poly-3-hydroxybutyrate/diethylene glycol copolymers / T. Volova [et al.] // Biotechnol. Prog. - 2016. - Vol. 32, Is. 4. - P1017-1028, DOI 10.1002/btpr.2267 . - ISSN 8756-7938
Кл.слова (ненормированные):
biocompatibility -- biosynthesis -- molecular weight -- physicochemical and mechanical properties -- poly(3-hydroxybutyrate)/diethylene glycol copolymers -- Biochemistry -- Biocompatibility -- Biosynthesis -- Cell culture -- Cell membranes -- Cells -- Cytology -- Fatty acids -- Glycols -- Molecular weight -- American Institute of Chemical Engineers -- Biological properties -- Chemical compositions -- Cytoplasmic membrane -- Degree of saturations -- Physico-chemical and mechanical properties -- Physiological effects -- Poly-3-hydroxybutyrate -- Biomechanics
Аннотация: This study investigates synthesis of poly(3-hydroxybutyrate)/diethylene glycol copolymers (P3HB/DEG) by Cupriavidus eutrophus B-10646 cells as related to DEG concentration in the medium and the time when it is added to the culture of cells synthesizing P3HB. The study determines the limits of physiological effect of DEG on C. eutrophus cells, showing that at DEG concentrations above 30 g/L, it inhibits cell growth, decreasing cell concentration and total P3HB/DEG yield and inducing an increase in the degree of saturation of fatty acids in lipids of cell cytoplasmic membrane. A series of copolymers containing different molar fractions of DEG (between 0.13 and 3.0 mol%) have been synthesized and their physicochemical, physical/mechanical, and biological properties have been investigated as related to the chemical composition and proportions of DEG monomers of the polymers. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1017–1028, 2016. © 2016 American Institute of Chemical Engineers

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Держатели документа:
Inst. of Biophysics SB RAS, Akademgorodok 50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Volova, T.; Zhila, N.; Kiselev, E.; Shishatskaya, E.

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The Chemical Basis of Fungal Bioluminescence / K. V. Purtov [et al.] // Angew. Chem. Int. Ed. - 2015. - Vol. 54, Is. 28. - P8124-8128, DOI 10.1002/anie.201501779 . - ISSN 1433-7851
Кл.слова (ненормированные):
bioluminescence -- bioorganic chemistry -- biosynthesis -- luciferin -- natural products -- Biochemistry -- Bioluminescence -- Biosynthesis -- Metabolites -- Phosphorescence -- Biochemical mechanisms -- Bioorganic chemistry -- luciferin -- Natural products -- Plant secondary metabolites -- Structural similarity -- Fungi
Аннотация: Many species of fungi naturally produce light, a phenomenon known as bioluminescence, however, the fungal substrates used in the chemical reactions that produce light have not been reported. We identified the fungal compound luciferin 3-hydroxyhispidin, which is biosynthesized by oxidation of the precursor hispidin, a known fungal and plant secondary metabolite. The fungal luciferin does not share structural similarity with the other eight known luciferins. Furthermore, it was shown that 3-hydroxyhispidin leads to bioluminescence in extracts from four diverse genera of luminous fungi, thus suggesting a common biochemical mechanism for fungal bioluminescence. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

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Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow, Russian Federation
Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Japan
Pirogov Russian National Research Medical University, Ostrovitianov 1, Moscow, Russian Federation

Доп.точки доступа:
Purtov, K.V.; Petushkov, V.N.; Baranov, M.S.; Mineev, K.S.; Rodionova, N.S.; Kaskova, Z.M.; Tsarkova, A.S.; Petunin, A.I.; Bondar, V.S.; Rodicheva, E.K.; Medvedeva, S.E.; Oba, Y.; Arseniev, A.S.; Lukyanov, S.; Gitelson, J.I.; Yampolsky, I.V.

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Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems [Text] / E. E. Maksimova, L. Y. Popova ; ed. a, EE Maksim // SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH : ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P. 1581-1586, DOI 10.1016/S0273-1177(01)00249-6. - Cited References: 17 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
MICROCOSMS
BACTERIA
Аннотация: Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Maksimova, E.E.; Popova, L.Y.; Maksim, a, EE \ed.\

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Cotranslational formation of active photoprotein obelin in a cell-free translation system: Direct ultrahigh sensitive measure of the translation course [Text] / N. G. Berestovskaya [et al.] // Anal. Biochem. - 1999. - Vol. 268, Is. 1. - P. 72-78, DOI 10.1006/abio.1998.3051. - Cited References: 22 . - ISSN 0003-2697

РУБ Biochemical Research Methods + Biochemistry & Molecular Biology + Chemistry, Analytical
Рубрики:
SEQUENCE-ANALYSIS
   MESSENGER-RNA
   CA-2+-ACTIVATED PHOTOPROTEIN
   LIGHT-EMISSION
   AEQUORIN
   CDNA
   CLONING
   EXPRESSION
Аннотация: Translation of apoobelin mRNA in a cell-free wheat germ translation system in the presence of coelenterazine and molecular oxygen results in cotranslational formation of active photoprotein. Active obelin formation is recorded by its luminescence, either direct in the translation mixture in the presence of coelenterazine and calcium ions or in aliquots from the translation mixture. In the second case translation is carried out with coelenterazine and EGTA. Registration of the translation course by luminescence of the synthesized product in both cases allows use of apoobelin mRNA at very low concentrations as an internal marker for immediate measure of protein biosynthesis activity of in vitro translation systems. It is shown that the simultaneous translation of any other mRNA does not affect translation of photoprotein mRNAs under standard conditions. Continuous registration of luminescence in a cuvette of a liquid scintillation counter in photon-counting mode varies the time of signal accumulation in a wide temporal range, thus increasing the numerical values of the recorded signals. Registration of photoprotein luminescence during translation can be used to obtain additional information about the translation process, for example codon reading speed, about protein folding, and about the formation of active proteins on ribosomes. (C) 1999 Academic Press.

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Держатели документа:
Russian Acad Sci, Branch Inst Bioorgan Chem, Pushchino 142292, Russia
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
Tech Univ Berlin, Inst Biochem & Mol Biol, D-10587 Berlin, Germany
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Berestovskaya, N.G.; Shaloiko, L.A.; Gorokhovatsky, A.Y.; Bondar, V.S.; Vysotski, E.S.; Maximov, J.E.; von Doehren, H...; Alakhov, Y.B.

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High-resolution structures of scytalone dehydratase-inhibitor complexes crystallized at physiological pH [Text] / Z. . Wawrzak [et al.] // Proteins. - 1999. - Vol. 35, Is. 4. - P. 425-439, DOI 10.1002/(SICI)1097-0134(19990601)35:4425::AID-PROT63.0.CO;2-1. - Cited References: 33 . - ISSN 0887-3585

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
MAGNAPORTHE-GRISEA
   HEMAGGLUTININ
   GLYCOPROTEIN
   REFINEMENT
   MELANIN
   DISEASE
   SITE
Кл.слова (ненормированные):
structure-based design -- enzyme inhibitors -- X-ray crystallography -- fungicides -- melanin biosynthesis
Аннотация: Scytalone dehydratase is a molecular target of inhibitor design efforts aimed at preventing the fungal disease caused by Magnaporthe grisea. A method for cocrystallization of enzyme with inhibitors at neutral pH has produced several crystal structures of enzyme-inhibitor complexes at resolutions ranging from 1.5 to 2.2 Angstrom Four high resolution structures of different enzyme-inhibitor complexes are described. In contrast to the original X-ray structure of the enzyme, the four new structures have well-defined electron density for the loop region comprising residues 115-119 and a different conformation between residues 154 and 160. The structure of the enzyme complex with an aminoquinazoline inhibitor showed that the inhibitor is in a position to form a hydrogen bond with the amide of the Asn131 side chain and with two water molecules in a fashion similar to the salicylamide inhibitor in the original structure, thus confirming design principles. The aminoquinazoline structure also allows for a more confident assignment of donors and accepters in the hydrogen bonding network, The structures of the enzyme complexes with two dichlorocyclopropane carboxamide inhibitors showed the two chlorine atoms nearly in plane with the amide side chain of Asn131. The positions of Phe53 and Phe158 are significantly altered in the new structures in comparison to the two structures obtained from crystals grown at acidic pH, The multiple structures help define the mobility of active site amino acids critical for catalysis and inhibitor binding. Proteins 1999;35:425-439. (C) 1999 Wiley-Liss, Inc.

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Держатели документа:
Dupont Co, Stine Haskell Res Ctr, Agr Prod, Newark, DE 19714 USA
Dupont Co, Expt Stn, Life Sci, Wilmington, DE USA
Karolinska Inst, Dept Med Biochem & Biophys, Stockholm, Sweden
Russian Acad Sci, Inst Biophys, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Wawrzak, Z...; Sandalova, T...; Steffens, J.J.; Basarab, G.S.; Lundqvist, T...; Lindqvist, Y...; Jordan, D.B.

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10.

Inhibitors of protein biosynthesis can stimulate proliferation of mouse hepatocytes in vitro [Text] / N. A. Setkov, A. V. Eremeev // Biol. Bull. - 2003. - Vol. 30, Is. 3. - P. 212-219, DOI 10.1023/A:1023843409416. - Cited References: 43 . - ISSN 1062-3590

РУБ Biology
Рубрики:
TRANSFORMING-GROWTH-FACTOR
   RAT-LIVER REGENERATION
   FACTOR-BETA
   DNA-SYNTHESIS
   PRIMARY CULTURE
   PARTIAL-HEPATECTOMY
   EPITHELIAL-CELLS
   EXPRESSION
   MECHANISMS
   MODULATION
Аннотация: Hepatocyte proliferation in the liver regenerating after partial hepatectomy ceases when the organ is restored, and the mechanism of this phenomenon is still unclear. In the experiments on fusing hepatocytes from the reoenerated mouse liver (15 days after partial hepatectomy) with NIH 3T3 mouse fibroblasts, we revealed no DNA synthesis in the nuclei of stimulated fibroblasts in heterokaryons (in the presence of hepatocyte nuclei), whereas DNA synthesis in nonfused cells was undisturbed. In this work, our purpose was to find out whether the suppression of DNA synthesis in heterokaryons could be due to the appearance in hepatocytes of some endogenous factors having an inhibitory effect on proliferation. To this end, hepatocytes from the mouse liver regenerated after partial hepatectomy were treated with cycloheximide for 1-4 h and were then fused with stimulated fibroblasts. Such a short-term treatment of hepatocytes with cycloheximide proved to result in the loss of their ability to inhibit DNA synthesis in the nuclei of stimulated or quiescent fibroblasts in heterokaryons, but hepatocytes proper actively proliferated in the medium with a low serum content (0.2%). When the mice with the liver reoenerated after partial hepatectomy were treated with a single sublethal dose of cycloheximide (3 mg/kg), their hepatocytes taken two days after this treatment had no inhibitory effect. Puromycin, another inhibitor of protein synthesis, had the same effect on hepatocytes. These results may be interpreted as evidence that the final stage of liver regeneration after damage is controlled by the factors having a C C negative effect on cell proliferation.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Setkov, N.A.; Eremeev, A.V.

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11.

Synthesis of biomass and utilization of plants wastes in a physical model of biological life-support system [Text] / A. A. Tikhomirov [et al.] // Acta Astronaut. - 2003. - Vol. 53: 53rd Congress of the International-Astronautical-Federation (IAF) (OCT 10, 2002, HOUSTON, TEXAS), Is. 04.10.2013. - P. 249-257, DOI 10.1016/S0094-5765(03)00137-1. - Cited References: 16 . - ISSN 0094-5765

РУБ Engineering, Aerospace

Аннотация: The paper considers problems of biosynthesis of higher plants' biomass and "bioloaical incineration" of plant wastes in a working physical model of biological LSS. The plant wastes are "biologically incinerated" in a special heterotrophic block involving Californian worms, mushrooms and straw. The block processes plant wastes (straw, haulms) to produce soil-like substrate (SLS) on which plants (wheat, radish) are grown. Gas exchange in such a system consists of respiratory gas exchange of SLS and photosynthesis and respiration of plants. Specifics of gas exchange dynamics of high plants - SLS complex has been considered. Relationship between such a gas exchange and PAR irradiance and age of plants has been established. Nitrogen and iron were found to the first to limit plants' growth on SLS when process conditions are deranged. The SLS microflora has been found to have different kinds of ammonifying and denitrifying bacteria which is indicative of intensive transformation of nitrogen-containing compounds. The number of physiological groups of microorganisms in SLS was, on the whole, steady. As a result, organic substances - products of exchange of plants and microorganisms were not accumulated in the medium, but mineralized and assimilated by the biocenosis. Experiments showed that the developed model of a manmade ecosystem realized complete utilization of plant wastes and involved them into the intrasystem turnover. (C) 2003 International Astronautical Federation. Published by Elsevier Science Ltd. All rights reserved.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
Univ B Pascal, Clermont Ferrand, France
Estec, ESA, Environm Control & Life Support Sect, Noordwijk, Netherlands
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Y.A.; Kovalev, V.S.; Gribovskaya, I.V.; Tirranen, L.S.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, C...

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12.

Recycling efficiencies of C,H,O,N,S, and P elements in a biological life support system based on microorganisms and higher plants [Text] / J. B. Gros [et al.] ; ed.: G Horneck, ME Vazquez, Vazquez, ME // SPACE LIFE SCIENCES: MISSIONS TO MARS, RADIATION BIOLOGY, AND PLANTS AS A FOUNDATION FOR LONG-TERM LIFE SUPPORT SYSTEMS IN SPACE. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: F0 1 and F1 3-F2 3 Symposia of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 1. - P. 195-199, DOI 10.1016/S0273-1177(02)00739-1. - Cited References: 10 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: MELiSSA is a microorganism based artificial ecosystem conceived as a tool for understanding the behavior of ecosystems and developing the technology for future Manned Space Missions. MELiSSA is composed of four compartments colonized by the microorganisms required by the function of this ecosystem : breakdown of waste produced by men, regeneration of atmosphere and biosynthesis of edible biomass. This paper reports the mass balance description of a Biological Life Support System composed of the MELiSSA loop and of a Higher Plant Compartment working in parallel with the photosynthetic Spirulina compartment producing edible biomass. The recycling efficiencies of the system are determined and compared for various working conditions of the MELiSSA loop with or without the HPC. (C) 2002 Published by Elsevier Science Ltd on behalf of COSPAR.

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Держатели документа:
Univ Clermont Ferrand, Lab Genie Chim & Biochim, F-63177 Aubiere, France
European Space Agcy, Estec, YVC, NL-2200 AG Noordwijk, Netherlands
RAS, Siberian Branch, Inst Biophys, Krasnoyarsk 630036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gros, J.B.; Poughon, L...; Lasseur, C...; Tikhomirov, A.A.; Horneck, G \ed.\; Vazquez, ME \ed.\

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13.

Biodegradable polymers - Perspectives and applications in agriculture / E. G. Kiselev, N. O. Zhila, T. G. Volova // IOP Conference Series: Earth and Environmental Science : IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст. 012036, DOI 10.1088/1755-1315/689/1/012036
Кл.слова (ненормированные):
Biodegradable polymers -- Ecosystems -- Fungi -- Glycerol -- Monounsaturated fatty acids -- Oilseeds -- Pesticides -- Substrates -- Sunflower oil -- Fenoxaprop-p-ethyl -- Natural materials -- Pesticide formulations -- Poly-3-hydroxybutyrate -- Polyhydroxyalkanoates -- Productive process -- Strategy of constructions -- Various substrates -- Palm oil
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.

Scopus
Держатели документа:
School of Fundamental Biology and Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics, SB, RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kiselev, E. G.; Zhila, N. O.; Volova, T. G.

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14.

Analysis of interactions between proteins and small-molecule drugs by a biosensor based on a graphene field-effect transistor / S. Xu, T. Wang, G. Liu [et al.] // Sens Actuators, B Chem. - 2021. - Vol. 326. - Ст. 128991, DOI 10.1016/j.snb.2020.128991 . - ISSN 0925-4005
Кл.слова (ненормированные):
Binding kinetics -- FET -- Imatinib -- LMW drugs -- Single-crystal graphene -- Biosensors -- Biosynthesis -- Drug interactions -- Graphene -- Graphene transistors -- Proteins -- Single crystals -- Graphene field-effect transistors -- Graphene sheets -- Interaction kinetics -- Linear response -- Low molecular weight drugs -- Real time -- Small-molecule drugs -- Target proteins -- Field effect transistors
Аннотация: We synthesized large-area single-crystal graphene sheets to use them in biosensors based on field-effect transistors (FET) for quantitative analysis of interaction kinetics and affinity between the imatinib drug and its target protein kinase Abl1. The G-FET biosensor showed an excellent performance and recognized imatinib at as low as 15.5 fM. The biosensor also showed a linear response to the logarithm of imatinib concentration in the 0.1 pM-10 ?M range. This graphene-based FET biosensor (G-FET) was also applied to quantify Abl1 Y253 F mutation and Abl1 dependency on Mg2+ to bind to imatinib in real-time. Results demonstrated in this work clearly showed that the novel G-FET biosensors are very promising to analyze interactions between proteins and low molecular weight drugs. © 2020 Elsevier B.V.

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Держатели документа:
Shandong Key Laboratory of Biophysics, Institute of Biophysics, Dezhou University, Dezhou, 253023, China
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, 660036, Russian Federation
Collaborative Innovation Center of Light Manipulations and Applications, Shandong Normal University, Jinan, 250358, China

Доп.точки доступа:
Xu, S.; Wang, T.; Liu, G.; Cao, Z.; Frank, L. A.; Jiang, S.; Zhang, C.; Li, Z.; Krasitskaya, V. V.; Li, Q.; Sha, Y.; Zhang, X.; Liu, H.; Wang, J.

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15.

Biophysics to Ecology / I. I. Gitel'zon // Her. Russ. Acad. Sci. - 2019. - Vol. 89, Is. 6. - P523-534, DOI 10.1134/S1019331619060066. - Cited References:25 . - ISSN 1019-3316. - ISSN 1555-6492

РУБ History & Philosophy Of Science + Multidisciplinary Sciences

Кл.слова (ненормированные):
biophysics -- biotechnology -- biosynthesis -- biopolymers -- bioluminescence -- bioluminescent analysis -- hydrogen biosynthesis -- noosphere -- life support -- closed ecosystem
Аннотация: This report presents some new methodological opportunities that biophysics can offer for solving the fundamental problem of planetary ecology-deciphering the mechanism that maintains the equilibrium state of the biosphere. The Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, where the author of this report works, is developing two complementary directions in ecological biophysics. The first is monitoring the vital activity of natural and constructed ecosystems by optical methods using the example of bioluminescence of the sea and closed ecosystems, and the second is studying the laws of parametric biosynthesis control and creating biotechnological control systems for these processes in order to construct noosphere-like ecosystems, in particular, for human life-support and survival systems in extreme conditions on the Earth and in space.

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Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.
Russian Acad Sci, Krasnoyarsk, Russia.

Доп.точки доступа:
Gitel'zon, I. I.

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16.

   Е071
   Б 63

    Волова, Татьяна Григорьевна.
    Управляемый биосинтез: от параметрически управляемых продуцирующих биосистем до новейших биофизических технологий [Текст] = Controlled biosynthesis: from parametrically controlled producing biosystems to newest biophysical technologies / Т. Г. Волова, Е. И. Шишацкая, Величко В. В. Трифонов С. В. // Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона / И. И. Гительзон, Т. Г. Волова, А. Г. Дегерменджи [и др.] ; ред., авт. предисл. Т. Г. Волова. - Новосибирск : Издательство Сибирского отделения Российской академии наук, 2019. - С. 129-148. - Библиогр.: с. 147-148 . - ISBN 978-5-7692-1650-3

УДК

577

574

ББК Е071я43 + Р252.0я43

Доп.точки доступа:
Гительзон, Иосиф Исаевич; Дегерменджи, Андрей Георгиевич; Дегерменджи, Н. Н.; Шевырногов, Анатолий Петрович; Кратасюк, В. А.; Барцев, Сергей иванович; Болсуновский, Александр Яковлевич; Бондарь, Владимир Антонович; Буров, А. Е.; Величко, В. В.; Гладышев, Михаил Иванович; Есимбекова, Е. Н.; Дементьев, Д. В.; Задереев, Егор Сергеевич; Зотина, Т. А.; Косиненко, Сергей Васильевич; Медведева, С. Е.; Петушков, В. Н.; Печуркин, Николай Савельевич; Прокопкин, И. Г.; Пузырь, А. П.; Пуртов, К. В.; Рогозин, Денис Юрьевич; Родионова, Н. С.; Ронжин, Н. О.; Сомова, Лидия Александровна; Тихомиров, Александр Аполлинариевич; Тихомирова, Наталья Александровна; Трифонов, С. В.; Ушакова, Софья Аврумовна; Франк, Л. А.; Хромечек, Е. Б.; Шишацкая, Е. И.; Шуваев, А. Н.; Шишацкая, Екатерина Игоревна; Трифонов С. В., Величко В. В.; Российская академия наук. Сибирское отделение; Институт биофизики(Красноярск)

Имеются экземпляры в отделах: всего 1 : ИБФ-КФ (1)
Свободны: ИБФ-КФ (1)

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17.

Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by cupriavidus necator B-10646 from mixtures of oleic acid and 3-hydroxyvalerate precursors / N. O. Zhila, G. S. Kalacheva, V. V. Fokht [и др.] // J. Sib. Fed. Univ. - Biol. - 2020. - Vol. 13, Is. 3. - С. 331-341, DOI 10.17516/1997-1389-0320 . - ISSN 1997-1389
Кл.слова (ненормированные):
3-hydroxyvalerate -- Cupriavidus necator -- Fatty acids -- Molecular weight -- oleic acid
Аннотация: Polyhydroxyalkanoates have attracted much attention as biodegradable alternative to petroleum-based synthetic plastics. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] copolymer is one of the best characterized PHA copolymers because of its high commercial potential. However, commercial use of PHAs has been limited by their high price. One approach to reducing the cost of PHA production is to use inexpensive carbon sources (fatty acids, plant oils, etc.). The aim of this work was to study synthesis of P(3HB-co-3HV) by the Cupriavidus necator B-10646 bacterium grown on oleic acid and different biochemical precursors of 3HV. Bacterial cells were grown for 72 h at 30°C and 200 rpm on an incubator shaker. Salts of propionic or valeric acids were used as precursors of 3HV. The content and the composition of the polymer were determined by gas chromatography of fatty acid methyl esters. Lipids and polymer were extracted from biomass using the method of Folch. The addition of potassium propionate and valerate did not inhibit bacterial growth and polymer synthesis, the cell concentration and polymer content reaching 9.3-9.5 g/L and 80-83%, respectively. The addition of potassium valerate or propionate led to the synthesis of (P(3HB-co-3HV)) copolymer containing 21.2 and 14.3 mol% of 3HV, respectively. The number average molecular weight (Mn) of the polymer synthesized by the bacterium on oleic acid alone was 220 kDa; the polydispersity of the polymer was 3.5. The polymer synthesized in the presence of potassium valerate and propionate was characterized by a lower Mn (156-178 kDa) and a higher polydispersity of the polymer (4.4-4.9). The main fatty acids (FA) of intracellular lipids were oleic (33.26% of the total FA) and palmitic acid (27.48% of the total FA). The addition of potassium propionate or valerate did not cause any significant changes in the composition of the FA of intracellular lipids of the strain studied. This study demonstrates the ability of C. necator B-10646 to synthesize P(3HB-co-3HV) from mixtures of oleic acid and 3HV precursors. The data obtained can be used to develop and implement an economically feasible process of the P(3HB-co-3HV) production. © Siberian Federal University. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Zhila, N. O.; Kalacheva, G. S.; Fokht, V. V.; Bubnova, S. S.; Volova, T. G.

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18.

Synthesis and characterization of multicomponent PHAs / E. G. Kiselev, A. D. Vasiliev, T. G. Volova // J. Sib. Fed. Univ. - Biol. - 2021. - Vol. 14, Is. 1. - С. 97-113, DOI 10.17516/1997-1389-0325. - Cited By :1 . - ISSN 1997-1389
Кл.слова (ненормированные):
Biosynthesis -- Copolymers -- Physicochemical properties -- Polyhydroxyalkanoates (PHAs) -- Precursor substrates
Аннотация: Cupriavidus necator B10646 bacterial cells were cultivated in the mode of synthesis of the reserve polyhydroxyalkanoates (PHAs) in the growth medium that contained, in addition to glucose as the main substrate, precursor substrates of the monomers of various monocarboxylic acids - salts of valeric and hexanoic acids, propionate, and ?-butyrolactone. PHA terpolymers and quaterpolymers with different compositions and proportions of monomers were synthesized, and their physicochemical properties were studied. The terpolymers were composed of monomers of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), or 3-hydroxyhexanoate (3HHx) and had the following compositions: P(3HB/3HB/4HHx) and P(3HB/3HV/4HHx). The quaterpolymers had the following composition: P(3HB/3HV/4HB/3HHx). All copolymer samples, regardless of the composition and proportions of monomers, had lower molecular weights and higher polydispersity values compared to the highly crystalline 3-hydroxybutyrate homopolymer, but retained thermal stability properties, with a difference between the melting point and thermal degradation of at least 100-110 °C. The inclusion of 3HV, 4HB, and 3HHx monomers in the C-chain of 3HB caused changes in the crystalline to amorphous phase ratio and a significant decrease in the degree of crystallinity (Cx), which depended on the type of monomers and their contents in the copolymer. The maximum decrease in Cx (9-17 %) was detected in the P(3HB/3HV/4HB) terpolymer and the P(3HB/3HV/4HB/3HHx) quaterpolymer (30-36 %). The study confirms that there is the possibility of synthesizing polymers with various compositions, including new ones, which differ significantly in their basic properties. © Siberian Federal University. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
L.V. Kirenskii Institute of Physics SB RAS, FRC Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kiselev, E. G.; Vasiliev, A. D.; Volova, T. G.

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19.

Natural-based polymers for biomedical applications / T. G. Volova [et al.] // : Apple Academic Press, 2017. - P1-439, DOI 10.1201/9781315366036
Аннотация: This new book presents the authors’ biomedical studies of natural degradable biopolymers (polyhydroxyalkanoates [PHAs]) and discusses the demand for medical-grade materials and modern trends, focusing on the present status and future potential of PHAs. The authors present and summarize their most important results and findings obtained during the last few years in experimental studies and clinical trials of PHAs at the Institute of Biophysics Siberian Branch of Russian Academy of Science. © 2017 by Apple Academic Press, Inc.

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Держатели документа:
Department of Biotechnology, Siberian Federal University, Krasnoyarsk, Russian Federation
Laboratory of Chemoautotrophic Biosynthesis, Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Russian Federation
Department of General Surgery, Krasnoyarsk State Medical School, Krasnoyarsk, Russian Federation
Department of Medical Biology, Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Krasnoyarsk, Russian Federation
Polymer Division, N. M. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russian Federation
Moscow State Academy of Fine Chemical Technology, Russian Federation
Kazan National Research Technological University, Kazan, Russian Federation

Доп.точки доступа:
Volova, T. G.; Vinnik, Y. S.; Shishatskaya, E. I.; Markelova, N. M.; Zaikov, G. E.

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20.

Biosynthesis and properties of P(3HB-co-3HV-co-3H4MV) produced by using the wild-type strain Cupriavidus eutrophus B-10646 / T. Volova [et al.] // Journal of Chemical Technology and Biotechnology. - 2018, DOI 10.1002/jctb.5763 . - Article in press. - ISSN 0268-2575
Кл.слова (ненормированные):
3-hydroxy-4-methylvalerate -- physicochemical properties -- polyhydroxyalkanoates -- synthesis -- Biochemistry -- Chemical industry -- Synthesis (chemical) -- 3-hydroxy-4-methylvalerate -- Crystalline-to-amorphous -- Degree of crystallinity -- Degrees of crystallinity -- Physicochemical property -- Polyhydroxyalkanoates -- Processing properties -- Wild-type strain -- Polymers
Аннотация: BACKGROUND: Polyhydroxyalkanoates (PHA) containing 3-hydroxy-4-methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3 HV-co-3H4MV)] by Cupriavidus eutrophus B-10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS: It was shown that 4-methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L?1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION: The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry

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Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
L.V. Kirenskii Institute of Physics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
Institute of Chemistry and Chemical Technology SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation
International and Inter University Centre of Nanoscience and Nanotechnology, Mahatam Gandhi University, Kottyam, India

Доп.точки доступа:
Volova, T.; Menshikova, O.; Zhila, N.; Vasiliev, A.; Kiselev, E.; Peterson, I.; Shishatskaya, E.; Thomas, S.

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