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Вид документа : Однотомное издание
Шифр издания : Е071/Б 63
Автор(ы) : Гительзон, Иосиф Исаевич, Волова, Татьяна Григорьевна, Дегерменджи, Андрей Георгиевич, Дегерменджи Н. Н., Шевырногов, Анатолий Петрович, Кратасюк В. А., Барцев, Сергей иванович, Болсуновский, Александр Яковлевич, Бондарь, Владимир Антонович, Буров А. Е., Величко В. В., Гладышев, Михаил Иванович, Есимбекова Е. Н., Дементьев Д. В., Есимбекова Е. Н., Задереев, Егор Сергеевич, Зотина Т. А., Косиненко, Сергей Васильевич, Медведева С. Е., Петушков В. Н., Печуркин, Николай Савельевич, Прокопкин И. Г., Пузырь А. П., Пуртов К. В., Рогозин, Денис Юрьевич, Родионова Н. С., Ронжин Н. О., Сомова, Лидия Александровна, Тихомиров, Александр Аполлинариевич, Тихомирова, Наталья Александровна, Трифонов С. В., Ушакова, Софья Аврумовна, Франк Л. А., Хромечек Е. Б., Шишацкая Е. И., Шуваев А. Н.
Заглавие : Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона
Выходные данные : Новосибирск: Издательство Сибирского отделения Российской академии наук, 2019
Колич.характеристики :292, [2] с.: ил., цв. ил.; 25 см.
Коллективы : Российская академия наук. Сибирское отделение, Институт биофизики (Красноярск)
Примечания : Рез. ст. англ. - Библиогр. в конце ст.
ISBN, Цена 978-5-7692-1650-3: 1635.00 р.
ГРНТИ : 31.27 + 76.03
УДК : 577 + 574 + 61
ББК : Е071я43 + Р252.0я43
Предметные рубрики: Экологическая биофизика
Медицинская биофизика
Ключевые слова (''Своб.индексиров.''): биолюминесценция--люцифераза--целентаразин--олигохеты--замкнутые экосистемы--управляемый биосинтез--наноалмазы--радиоизотопные методы--биосфера--жирные кислоты--системы жизнеобеспечения--меромиктические озера--геобиосфера--эволюция--глобальный климат--медицинская биофизика
Содержание : Краткий очерк истории, состояния и перспектив/ И. И. Гительзон. Коллекция культур ибсо как база для исследований биолюминесценции й и грибов в ИБФ СО РАН/ С. Е. Медведева. Биолюминесценция Мирового океана/ И. И. Гительзон, Л. А. Левин, А. С. Артемкин, Р. Н., Чепилов В. В., Молвинских С.Л., Черепанов О. А., Чугунов Ю. В., Караев Н. Д., Загородний Ю. А., Шевырногов А. П. Утюшев Р. Н. Бактериальная люцифераза в биолюминесцентном анализе/ В. А. Кратасюк, Е. Н. Есимбекова. Целентеразин-зависимые биолюминесцентные системы/ Л. А. Франк. Изучение химического механизма биолюминесценции грибов/ К. В. Пуртов, В. Н. Петушков, Н. С. Родионова. Исследование биолюминесценции сибирских почвенных олигохет/ Н. С. Родионова, А. А. Петушков. Экспериментальные модели замкнутых экосистем с расчетной долей человека как перспективное направление исследований по созданию биолого-технической системы жизнеобеспечения/ А. А. Тихомиров, С. А. Ушакова, Н. А. Тихомирова, С. В., Величко В. В. Трифонов С. В. Управляемый биосинтез: от параметрически управляемых продуцирующих биосистем до новейших биофизических технологий/ Т. Г. Волова, Е. И. Шишацкая. Биомедицинские приложения наноалмазов взрывного синтеза/ В. С. Бондарь, А. П. Пузырь, Н. О. Ронжин, А. В., Буров А. Е. Барон А. В. Применение радиоизотопных методов в институте биофизики СО РАН: от клеток крови до экосистем/ А. Я. Болсуновский, С. В. Косиненко, Т. А. Зотина, Д. В. Дементьев. Биосфера - взгляд сверху (экспрессные методы мониторинга биосферы в ИБФ СО РАН – ХХ–ХХI вв.)/ А. П. Шевырногов. Жирные кислоты в экологической биофизике водных систем/ М. И. Гладышев. Сравнительное исследование устойчивости стратификации и структуры трофической сети в меромиктических озерах Шира и Шунет (Южная Сибирь, Россия)/ Д. Ю. Рогозин, Е. С. Задереев, И. Г. Прокопкин [и др.]. Непрерывный рост интенсивности энерго-вещественных взаимодействий в эволюции геобиосферы Земли/ Н. С. Печуркин, А. Н. Шуваев, Л. А. Сомова. Малоразмерные модели биосферы и феноменология изменения глобального климата/ С. И. Барцев, А. Г. Дегерменджи. Направления развития биофизики в Красноярске/ А. Г. Дегерменджи.
Аннотация: Сборник посвящен широкому кругу исследований в области экологической биофизики – научного направления на стыке наук – от исследований на молекулярном уровне до вопросов управления большими природными экосистемами. Рассмотрены исторические вехи развития экологического направления биофизики. Основной акцент сборника основан на современных, актуальных достижениях красноярских биофизиков, которым удалось сохранить и развить многоплановые направления, которые были заложены в 50-х гг. ХХ века И. И. Гительзоном. Наряду с обзорными материалами и результатами фундаментальных исследований представлен ряд готовых к внедрению биотехнологий. Книга адресована биофизикам, экологам и химикам, а также преподавателям и студентам биофизических, биологических и экологических кафедр университетов.
Экземпляры :ИБФ-КФ(1)
Свободны : ИБФ-КФ(1)
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Gorbunova O.V., Zhila N.O.
Заглавие : Dynamics of activity of the key enzymes of polyhydroxyalkanoate metabolism in Ralstonia eutropha B5786
Колич.характеристики :8 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 2004. - Vol. 40, Is. 2. - P170-177. - ISSN 0003-6838, DOI 10.1023/B:ABIM.0000018921.04863.d5
Примечания : Cited References: 27
Предметные рубрики: POLY-BETA-HYDROXYBUTYRATE
ORGANISM ALCALIGENES-EUTROPHUS
ESCHERICHIA-COLI
PHB
POLY(3-HYDROXYBUTYRATE)
BIOSYNTHESIS
CLONING
GENES
CHAIN
H16
Аннотация: The dynamics of accumulation of polyhydroxybutyrate (PHB) and the activities of key enzymes of PHB metabolism (beta-ketothiolase, acetoacetyl-CoA reductase, PHB synthase, D-hydroxybutyrate dehydrogenase, and PHB depolymerase) in the hydrogen bacterium Ralstonia eutropha B5786 were studied under various conditions of carbon nutrition and substrate availability. The highest activities of beta-ketothiolase, acetoacetyl-CoA reductase, and PHB synthase were recorded during acceleration of PHB synthesis. The activities of enzymes catalyzing PHB depolymerization (PHB depolymerase and D-hydroxybutyrate dehydrogenase) were low, being expressed only upon stimulated endogenous PHB degradation. The change of carbon source (CO2 or fructose) did not affect the time course of the enzyme activity significantly.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bauer S..., Schott A.K., Illarionova V..., Bacher A..., Huber R..., Fischer M...
Заглавие : Biosynthesis of tetrahydrofolate in plants: Crystal structure of 7,8-dihydroneopterin aldolase from Arabidopsis thaliana reveals a novel adolase class
Колич.характеристики :13 с
Место публикации : J. Mol. Biol.: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 2004. - Vol. 339, Is. 4. - P967-979. - ISSN 0022-2836, DOI 10.1016/j.jmb.2004.04.034
Примечания : Cited References: 66
Предметные рубрики: GTP CYCLOHYDROLASE-I
GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE
6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE
ESCHERICHIA-COLI
DIHYDRONEOPTERIN ALDOLASE
FOLIC-ACID
ENZYMATIC SYNTHESIS
DIHYDROPTEROATE SYNTHASE
REACTION-MECHANISM
3-DIMENSIONAL STRUCTURE
Ключевые слова (''Своб.индексиров.''): tetrahydrofolate biosynthesis--aldolase classes--retroaldol reaction--purin binding--schiff base
Аннотация: Dihydroneopterin aldolase (DHNA) catalyses a retroaldol reaction yielding 6-hydroxymethyl-7,8-dihydropterin, a biosynthetic precursor of the vitamin, tetrahydrofolate. The enzyme is a potential target for antimicrobial and anti-parasite chemotherapy. A gene specifying a dihydroneopterin aldolase from Arabidopsis thaliana was expressed in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity and crystallised using polyethylenglycol as the precipitating agent. The crystal structure was solved by X-ray diffraction analysis at 2.2 Angstrom resolution. The enzyme forms a D-4-symmetric homo-octamer. Each polypeptide chain is folded into a single domain comprising an antiparallel four-stranded beta-sheet and two long alpha-helices. Four monomers are arranged in a tetrameric ring, and two of these rings form a hollow cylinder. Well defined purine derivatives are found at all eight topologically equivalent active sites. The subunit fold of the enzyme is related to substructures of dihydroneopterin triphosphate epimerase, GTP cyclohydrolase I, and pyruvoyltetrahydropterin synthase, which are all involved in the biosynthesis of pteridine type cofactors, and to urate oxidase, although some members of that superfamily have no detectable sequence similarity Due to structural and mechanistical differences of DHNA in comparison with class I and class II aldolases, a new aldolase class is proposed. (C) 2004 Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Purtov K.V., Petushkov V.N., Baranov M.S., Mineev K.S., Rodionova N.S., Kaskova Z.M., Tsarkova A.S., Petunin A.I., Bondar V.S., Rodicheva E.K., Medvedeva S.E., Oba Y., Oba Y., Arseniev A.S., Lukyanov S., Gitelson J.I., Yampolsky I.V.
Заглавие : The Chemical Basis of Fungal Bioluminescence
Место публикации : Angew. Chem. Int. Ed. - 2015. - Vol. 54, Is. 28. - С. 8124-8128. - ISSN 14337851 (ISSN) , DOI 10.1002/anie.201501779
Ключевые слова (''Своб.индексиров.''): bioluminescence--bioorganic chemistry--biosynthesis--luciferin--natural products--biochemistry--bioluminescence--biosynthesis--metabolites--phosphorescence--biochemical mechanisms--bioorganic chemistry--luciferin--natural products--plant secondary metabolites--structural similarity--fungi
Аннотация: Many species of fungi naturally produce light, a phenomenon known as bioluminescence, however, the fungal substrates used in the chemical reactions that produce light have not been reported. We identified the fungal compound luciferin 3-hydroxyhispidin, which is biosynthesized by oxidation of the precursor hispidin, a known fungal and plant secondary metabolite. The fungal luciferin does not share structural similarity with the other eight known luciferins. Furthermore, it was shown that 3-hydroxyhispidin leads to bioluminescence in extracts from four diverse genera of luminous fungi, thus suggesting a common biochemical mechanism for fungal bioluminescence. © 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Gitelson J. I., Degermendzhy A. G.
Заглавие : Evolution and present status of experimental manned ecological systems for long-term human life support - Bios, developed by the institute of biophysics of Russian academy of sciences in Krasnoyarsk (Siberia)
Место публикации : Proceedings of the International Astronautical Congress, IAC: International Astronautical Federation, IAF, 2015. - Vol. 1: 66th International Astronautical Congress 2015: Space - The Gateway for Mankind's Future, IAC 2015 (12 October 2015 through 16 October 2015, ) Conference code: 122921. - С. 243-250
Ключевые слова (''Своб.индексиров.''): arid regions--biochemistry--biospherics--plant shutdowns--proteins--reconfigurable hardware--closed ecological systems--corrective actions--essential proteins--extreme conditions--human intelligence--long-term experiments--physiological effects--russian academy of sciences--ecology
Аннотация: Closed ecological systems are of two-fold interest - as models of the Earth's biosphere explorable in experiments and as a facility for long-term autonomous human life support beyond the Earth. Theoretical analysis and experimental implementation of highly closed manned systems has been the subject of studies at the Institute of Biophysics (Russian Academy of Sciences, Siberian Branch) for many years. BIOS systems of increasing complexity with complete regeneration of atmosphere, water and partially food have been realized. In BIOS-3 experiments the system inhabited by 2-3 researchers for 4-6 months maintained its metabolic equilibrium without any negative physiological effect on the crew, which proves its sustainable condition. Specific for BIOS-3 is internal control by the people inhabiting the system. So, BIOS-3 is the first experimental implementation of V.l. Vernadsky's idea about the noosphere - habitable Biosphere controlled by human intelligence. Contrary to predictions of many environmentalists the closedness of the ecosystem is a factor that does not reduce, but increases its sustainability and makes its use for reliable life support outside the Earth realistic. The system is sustainable owing to permanent feedback between the monitoring of few key parameters of the system and automatic corrective actions on them. Main object of control is photo-biosynthesis regenerating parameters of human habitat disturbed by his vital activities. This principle has been realized in BIOS system and proved its reliability in long-term experiments. A new challenge is specified-optimal increase of trophic closedness of the system by reproduction within it essential proteins (peptides and amino acids), lipids, vitamins and other essential compounds. Alternative lines of attack on this problem by state-of-the-art biotechnological methods, GMO including, are under analysis. Reduced BIOS version - without complete closure - can be a breakthrough instrument to improve the quality of life of people living under extreme conditions on the Earth - in polar latitudes (Arctic, Antarctic), in deserts, in high mountains.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Zhila N., Kiselev E., Shishatskaya E.
Заглавие : A study of synthesis and properties of poly-3-hydroxybutyrate/diethylene glycol copolymers
Место публикации : Biotechnol. Prog.: John Wiley and Sons Inc., 2016. - Vol. 32, Is. 4. - С. 1017-1028. - ISSN 87567938 (ISSN) , DOI 10.1002/btpr.2267
Ключевые слова (''Своб.индексиров.''): biocompatibility--biosynthesis--molecular weight--physicochemical and mechanical properties--poly(3-hydroxybutyrate)/diethylene glycol copolymers--biochemistry--biocompatibility--biosynthesis--cell culture--cell membranes--cells--cytology--fatty acids--glycols--molecular weight--american institute of chemical engineers--biological properties--chemical compositions--cytoplasmic membrane--degree of saturations--physico-chemical and mechanical properties--physiological effects--poly-3-hydroxybutyrate--biomechanics
Аннотация: This study investigates synthesis of poly(3-hydroxybutyrate)/diethylene glycol copolymers (P3HB/DEG) by Cupriavidus eutrophus B-10646 cells as related to DEG concentration in the medium and the time when it is added to the culture of cells synthesizing P3HB. The study determines the limits of physiological effect of DEG on C. eutrophus cells, showing that at DEG concentrations above 30 g/L, it inhibits cell growth, decreasing cell concentration and total P3HB/DEG yield and inducing an increase in the degree of saturation of fatty acids in lipids of cell cytoplasmic membrane. A series of copolymers containing different molar fractions of DEG (between 0.13 and 3.0 mol%) have been synthesized and their physicochemical, physical/mechanical, and biological properties have been investigated as related to the chemical composition and proportions of DEG monomers of the polymers. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1017–1028, 2016. © 2016 American Institute of Chemical Engineers
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinov N.A.
Заглавие : Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha grown under conditions favoring polyhydroxybutyrate biosynthesis
Колич.характеристики :5 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 2003. - Vol. 39, Is. 2. - P166-170. - ISSN 0003-6838, DOI 10.1023/A:1022585912873
Примечания : Cited References: 15
Предметные рубрики: ALCALIGENES
Аннотация: Kinetic parameters of a culture of the hydrogen-oxidizing bacterium Ralstonia eutropha grown on a gas substrate under conditions favoring autotrophic biosynthesis of polyhydroxybutyrate were studied. The following parameters, making it possible to control and optimize the process in industrial situations, were determined: specific rate of substrate consumption, physical properties of the culture medium, and coefficients of heat emission and mass transfer.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Maksimova E.E., Popova L.Y.
Заглавие : Expression of cloned genes of transgenic microorganisms introduced into man-made ecosystems
Колич.характеристики :6 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH: ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P1581-1586. - ISBN 0273-1177, DOI 10.1016/S0273-1177(01)00249-6
Примечания : Cited References: 17
Предметные рубрики: MICROCOSMS
BACTERIA
Аннотация: Modeling of transgenic microorganism introduction into small man-made ecosystems can help forecast changes in expression of cloned genes under different conditions of existence. Introduction of the E. coli Z905/pPHL7 strain containing a plasmid with luminescent system genes of luminous bacteria led to changes in cell and colony morphology, reduction in metabolic activity of cells, and, as a result, a lower level of expression of cloned gene. A low concentration of nutrients has been shown to favor greatly the phenotypic change of cells of the recombinant strain. Expression of cloned genes changed due to: a lower concentration of plasmid DNA, a change in regulation of cloned genes, and a change in cells of biosynthesis of substrates needed for expression of luminescent genes. The conducted investigations can provide a basis for the use of marker transgenic microorganisms in closed ecosystems of different types. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Berestovskaya N.G., Shaloiko L.A., Gorokhovatsky A.Y., Bondar V.S., Vysotski E.S., Maximov J.E., von Doehren H..., Alakhov Y.B.
Заглавие : Cotranslational formation of active photoprotein obelin in a cell-free translation system: Direct ultrahigh sensitive measure of the translation course
Колич.характеристики :7 с
Место публикации : Anal. Biochem.: ACADEMIC PRESS INC, 1999. - Vol. 268, Is. 1. - P72-78. - ISSN 0003-2697, DOI 10.1006/abio.1998.3051
Примечания : Cited References: 22
Предметные рубрики: SEQUENCE-ANALYSIS
MESSENGER-RNA
CA-2+-ACTIVATED PHOTOPROTEIN
LIGHT-EMISSION
AEQUORIN
CDNA
CLONING
EXPRESSION
Аннотация: Translation of apoobelin mRNA in a cell-free wheat germ translation system in the presence of coelenterazine and molecular oxygen results in cotranslational formation of active photoprotein. Active obelin formation is recorded by its luminescence, either direct in the translation mixture in the presence of coelenterazine and calcium ions or in aliquots from the translation mixture. In the second case translation is carried out with coelenterazine and EGTA. Registration of the translation course by luminescence of the synthesized product in both cases allows use of apoobelin mRNA at very low concentrations as an internal marker for immediate measure of protein biosynthesis activity of in vitro translation systems. It is shown that the simultaneous translation of any other mRNA does not affect translation of photoprotein mRNAs under standard conditions. Continuous registration of luminescence in a cuvette of a liquid scintillation counter in photon-counting mode varies the time of signal accumulation in a wide temporal range, thus increasing the numerical values of the recorded signals. Registration of photoprotein luminescence during translation can be used to obtain additional information about the translation process, for example codon reading speed, about protein folding, and about the formation of active proteins on ribosomes. (C) 1999 Academic Press.
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10.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Gros J.B., Poughon L..., Lasseur C..., Tikhomirov A.A.
Заглавие : Recycling efficiencies of C,H,O,N,S, and P elements in a biological life support system based on microorganisms and higher plants
Колич.характеристики :5 с
Место публикации : SPACE LIFE SCIENCES: MISSIONS TO MARS, RADIATION BIOLOGY, AND PLANTS AS A FOUNDATION FOR LONG-TERM LIFE SUPPORT SYSTEMS IN SPACE. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: F0 1 and F1 3-F2 3 Symposia of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 1. - P195-199. - ISBN 0273-1177, DOI 10.1016/S0273-1177(02)00739-1
Примечания : Cited References: 10
Аннотация: MELiSSA is a microorganism based artificial ecosystem conceived as a tool for understanding the behavior of ecosystems and developing the technology for future Manned Space Missions. MELiSSA is composed of four compartments colonized by the microorganisms required by the function of this ecosystem : breakdown of waste produced by men, regeneration of atmosphere and biosynthesis of edible biomass. This paper reports the mass balance description of a Biological Life Support System composed of the MELiSSA loop and of a Higher Plant Compartment working in parallel with the photosynthetic Spirulina compartment producing edible biomass. The recycling efficiencies of the system are determined and compared for various working conditions of the MELiSSA loop with or without the HPC. (C) 2002 Published by Elsevier Science Ltd on behalf of COSPAR.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Setkov N.A., Eremeev A.V.
Заглавие : Inhibitors of protein biosynthesis can stimulate proliferation of mouse hepatocytes in vitro
Колич.характеристики :8 с
Место публикации : Biol. Bull: MAIK NAUKA/INTERPERIODICA, 2003. - Vol. 30, Is. 3. - P212-219. - ISSN 1062-3590, DOI 10.1023/A:1023843409416
Примечания : Cited References: 43
Предметные рубрики: TRANSFORMING-GROWTH-FACTOR
RAT-LIVER REGENERATION
FACTOR-BETA
DNA-SYNTHESIS
PRIMARY CULTURE
PARTIAL-HEPATECTOMY
EPITHELIAL-CELLS
EXPRESSION
MECHANISMS
MODULATION
Аннотация: Hepatocyte proliferation in the liver regenerating after partial hepatectomy ceases when the organ is restored, and the mechanism of this phenomenon is still unclear. In the experiments on fusing hepatocytes from the reoenerated mouse liver (15 days after partial hepatectomy) with NIH 3T3 mouse fibroblasts, we revealed no DNA synthesis in the nuclei of stimulated fibroblasts in heterokaryons (in the presence of hepatocyte nuclei), whereas DNA synthesis in nonfused cells was undisturbed. In this work, our purpose was to find out whether the suppression of DNA synthesis in heterokaryons could be due to the appearance in hepatocytes of some endogenous factors having an inhibitory effect on proliferation. To this end, hepatocytes from the mouse liver regenerated after partial hepatectomy were treated with cycloheximide for 1-4 h and were then fused with stimulated fibroblasts. Such a short-term treatment of hepatocytes with cycloheximide proved to result in the loss of their ability to inhibit DNA synthesis in the nuclei of stimulated or quiescent fibroblasts in heterokaryons, but hepatocytes proper actively proliferated in the medium with a low serum content (0.2%). When the mice with the liver reoenerated after partial hepatectomy were treated with a single sublethal dose of cycloheximide (3 mg/kg), their hepatocytes taken two days after this treatment had no inhibitory effect. Puromycin, another inhibitor of protein synthesis, had the same effect on hepatocytes. These results may be interpreted as evidence that the final stage of liver regeneration after damage is controlled by the factors having a C C negative effect on cell proliferation.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tikhomirov A.A., Ushakova S.A., Manukovsky N.S., Lisovsky G.M., Kudenko Y.A., Kovalev V.S., Gribovskaya I.V., Tirranen L.S., Zolotukhin I.G., Gros J.B., Lasseur C...
Заглавие : Synthesis of biomass and utilization of plants wastes in a physical model of biological life-support system
Колич.характеристики :9 с
Место публикации : Acta Astronaut.: PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 53: 53rd Congress of the International-Astronautical-Federation (IAF) (OCT 10, 2002, HOUSTON, TEXAS), Is. 04.10.2013. - P249-257. - ISSN 0094-5765, DOI 10.1016/S0094-5765(03)00137-1
Примечания : Cited References: 16
Аннотация: The paper considers problems of biosynthesis of higher plants' biomass and "bioloaical incineration" of plant wastes in a working physical model of biological LSS. The plant wastes are "biologically incinerated" in a special heterotrophic block involving Californian worms, mushrooms and straw. The block processes plant wastes (straw, haulms) to produce soil-like substrate (SLS) on which plants (wheat, radish) are grown. Gas exchange in such a system consists of respiratory gas exchange of SLS and photosynthesis and respiration of plants. Specifics of gas exchange dynamics of high plants - SLS complex has been considered. Relationship between such a gas exchange and PAR irradiance and age of plants has been established. Nitrogen and iron were found to the first to limit plants' growth on SLS when process conditions are deranged. The SLS microflora has been found to have different kinds of ammonifying and denitrifying bacteria which is indicative of intensive transformation of nitrogen-containing compounds. The number of physiological groups of microorganisms in SLS was, on the whole, steady. As a result, organic substances - products of exchange of plants and microorganisms were not accumulated in the medium, but mineralized and assimilated by the biocenosis. Experiments showed that the developed model of a manmade ecosystem realized complete utilization of plant wastes and involved them into the intrasystem turnover. (C) 2003 International Astronautical Federation. Published by Elsevier Science Ltd. All rights reserved.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Yampolsky I.
Заглавие : Fungal bioluminescence system: luciferin, luciferase and luciferin biosynthesis
Колич.характеристики :1 с
Коллективы : Russian Science Foundation [16-14-00052]
Место публикации : FEBS J.: WILEY, 2017. - Vol. 284: 42nd Congress of the Federation-of-European-Biochemical-Societies (FEBS) (SEP 10-14, 2017, Jerusalem, ISRAEL). - С. 189-189. - ISSN 1742-464X. - ISSN 1742-4658(eISSN)
Примечания : Cited References:0. - This work was supported by the Russian Science Foundation grant 16-14-00052.
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14.

Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Kiselev E. G., Zhila N. O., Volova T. G.
Заглавие : Biodegradable polymers - Perspectives and applications in agriculture
Место публикации : IOP Conference Series: Earth and Environmental Science: IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст.012036. - , DOI 10.1088/1755-1315/689/1/012036
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Xu S., Wang T., Liu G., Cao Z., Frank L. A., Jiang S., Zhang C., Li Z., Krasitskaya V. V., Li Q., Sha Y., Zhang X., Liu H., Wang J.
Заглавие : Analysis of interactions between proteins and small-molecule drugs by a biosensor based on a graphene field-effect transistor
Место публикации : Sens Actuators, B Chem: Elsevier B.V., 2021. - Vol. 326. - Ст.128991. - ISSN 09254005 (ISSN), DOI 10.1016/j.snb.2020.128991
Аннотация: We synthesized large-area single-crystal graphene sheets to use them in biosensors based on field-effect transistors (FET) for quantitative analysis of interaction kinetics and affinity between the imatinib drug and its target protein kinase Abl1. The G-FET biosensor showed an excellent performance and recognized imatinib at as low as 15.5 fM. The biosensor also showed a linear response to the logarithm of imatinib concentration in the 0.1 pM-10 ?M range. This graphene-based FET biosensor (G-FET) was also applied to quantify Abl1 Y253 F mutation and Abl1 dependency on Mg2+ to bind to imatinib in real-time. Results demonstrated in this work clearly showed that the novel G-FET biosensors are very promising to analyze interactions between proteins and low molecular weight drugs. © 2020 Elsevier B.V.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kiselev E. G., Vasiliev A. D., Volova T. G.
Заглавие : Synthesis and characterization of multicomponent PHAs
Место публикации : J. Sib. Fed. Univ. - Biol.: Siberian Federal University, 2021. - Vol. 14, Is. 1. - С. 97-113. - ISSN 19971389 (ISSN), DOI 10.17516/1997-1389-0325
Примечания : Cited By :1
Аннотация: Cupriavidus necator B10646 bacterial cells were cultivated in the mode of synthesis of the reserve polyhydroxyalkanoates (PHAs) in the growth medium that contained, in addition to glucose as the main substrate, precursor substrates of the monomers of various monocarboxylic acids - salts of valeric and hexanoic acids, propionate, and ?-butyrolactone. PHA terpolymers and quaterpolymers with different compositions and proportions of monomers were synthesized, and their physicochemical properties were studied. The terpolymers were composed of monomers of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), or 3-hydroxyhexanoate (3HHx) and had the following compositions: P(3HB/3HB/4HHx) and P(3HB/3HV/4HHx). The quaterpolymers had the following composition: P(3HB/3HV/4HB/3HHx). All copolymer samples, regardless of the composition and proportions of monomers, had lower molecular weights and higher polydispersity values compared to the highly crystalline 3-hydroxybutyrate homopolymer, but retained thermal stability properties, with a difference between the melting point and thermal degradation of at least 100-110 °C. The inclusion of 3HV, 4HB, and 3HHx monomers in the C-chain of 3HB caused changes in the crystalline to amorphous phase ratio and a significant decrease in the degree of crystallinity (Cx), which depended on the type of monomers and their contents in the copolymer. The maximum decrease in Cx (9-17 %) was detected in the P(3HB/3HV/4HB) terpolymer and the P(3HB/3HV/4HB/3HHx) quaterpolymer (30-36 %). The study confirms that there is the possibility of synthesizing polymers with various compositions, including new ones, which differ significantly in their basic properties. © Siberian Federal University. All rights reserved.
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17.

Вид документа : Статья из сборника (однотомник)
Шифр издания : Е071/Б 63
Автор(ы) : Волова, Татьяна Григорьевна, Шишацкая, Екатерина Игоревна, Трифонов С. В., Величко В. В.
Заглавие : Управляемый биосинтез: от параметрически управляемых продуцирующих биосистем до новейших биофизических технологий
Параллельн. заглавия :Controlled biosynthesis: from parametrically controlled producing biosystems to newest biophysical technologies
Коллективы : Российская академия наук, Институт биофизики
Место публикации : Биофизика для экологии и медицины: к 90-летию академика РАН И. И. Гительзона/ И. И. Гительзон, Т. Г. Волова, А. Г. Дегерменджи [и др.] ; ред., авт. предисл. Т. Г. Волова. - Новосибирск: Издательство Сибирского отделения Российской академии наук, 2019. - С. 129-148. - ISBN 978-5-7692-1650-3 (Шифр Е071/Б 63-478048446)
Примечания : Библиогр.: с. 147-148
УДК : 577 + 574 + 61
ББК : Е071я43 + Р252.0я43
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N. O., Kalacheva G. S., Fokht V. V., Bubnova S. S., Volova T. G.
Заглавие : Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by cupriavidus necator B-10646 from mixtures of oleic acid and 3-hydroxyvalerate precursors
Место публикации : J. Sib. Fed. Univ. - Biol.: Siberian Federal University, 2020. - Vol. 13, Is. 3. - С. 331-341. - ISSN 19971389 (ISSN), DOI 10.17516/1997-1389-0320
Аннотация: Polyhydroxyalkanoates have attracted much attention as biodegradable alternative to petroleum-based synthetic plastics. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] copolymer is one of the best characterized PHA copolymers because of its high commercial potential. However, commercial use of PHAs has been limited by their high price. One approach to reducing the cost of PHA production is to use inexpensive carbon sources (fatty acids, plant oils, etc.). The aim of this work was to study synthesis of P(3HB-co-3HV) by the Cupriavidus necator B-10646 bacterium grown on oleic acid and different biochemical precursors of 3HV. Bacterial cells were grown for 72 h at 30°C and 200 rpm on an incubator shaker. Salts of propionic or valeric acids were used as precursors of 3HV. The content and the composition of the polymer were determined by gas chromatography of fatty acid methyl esters. Lipids and polymer were extracted from biomass using the method of Folch. The addition of potassium propionate and valerate did not inhibit bacterial growth and polymer synthesis, the cell concentration and polymer content reaching 9.3-9.5 g/L and 80-83%, respectively. The addition of potassium valerate or propionate led to the synthesis of (P(3HB-co-3HV)) copolymer containing 21.2 and 14.3 mol% of 3HV, respectively. The number average molecular weight (Mn) of the polymer synthesized by the bacterium on oleic acid alone was 220 kDa; the polydispersity of the polymer was 3.5. The polymer synthesized in the presence of potassium valerate and propionate was characterized by a lower Mn (156-178 kDa) and a higher polydispersity of the polymer (4.4-4.9). The main fatty acids (FA) of intracellular lipids were oleic (33.26% of the total FA) and palmitic acid (27.48% of the total FA). The addition of potassium propionate or valerate did not cause any significant changes in the composition of the FA of intracellular lipids of the strain studied. This study demonstrates the ability of C. necator B-10646 to synthesize P(3HB-co-3HV) from mixtures of oleic acid and 3HV precursors. The data obtained can be used to develop and implement an economically feasible process of the P(3HB-co-3HV) production. © Siberian Federal University. All rights reserved.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitel'zon I. I.
Заглавие : Biophysics to Ecology
Колич.характеристики :12 с
Место публикации : Her. Russ. Acad. Sci.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2019. - Vol. 89, Is. 6. - С. 523-534. - ISSN 1019-3316, DOI 10.1134/S1019331619060066. - ISSN 1555-6492(eISSN)
Примечания : Cited References:25
Аннотация: This report presents some new methodological opportunities that biophysics can offer for solving the fundamental problem of planetary ecology-deciphering the mechanism that maintains the equilibrium state of the biosphere. The Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, where the author of this report works, is developing two complementary directions in ecological biophysics. The first is monitoring the vital activity of natural and constructed ecosystems by optical methods using the example of bioluminescence of the sea and closed ecosystems, and the second is studying the laws of parametric biosynthesis control and creating biotechnological control systems for these processes in order to construct noosphere-like ecosystems, in particular, for human life-support and survival systems in extreme conditions on the Earth and in space.
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20.

Вид документа :
Шифр издания :
Автор(ы) : Volova T. G., Vinnik Y. S., Shishatskaya E. I., Markelova N. M., Zaikov G. E.
Заглавие : Natural-based polymers for biomedical applications
Место публикации :: Apple Academic Press, 2017. - С. 1-439. - , DOI 10.1201/9781315366036
Аннотация: This new book presents the authors’ biomedical studies of natural degradable biopolymers (polyhydroxyalkanoates [PHAs]) and discusses the demand for medical-grade materials and modern trends, focusing on the present status and future potential of PHAs. The authors present and summarize their most important results and findings obtained during the last few years in experimental studies and clinical trials of PHAs at the Institute of Biophysics Siberian Branch of Russian Academy of Science. © 2017 by Apple Academic Press, Inc.
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