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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T..., Kiselev E..., Vinogradova O..., Nikolaeva E..., Chistyakov A..., Sukovatiy A..., Shishatskaya E...
Заглавие : A Glucose-Utilizing Strain, Cupriavidus euthrophus B-10646: Growth Kinetics, Characterization and Synthesis of Multicomponent PHAs
Колич.характеристики :15 с
Коллективы : Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" [11.G34.31.0013]
Место публикации : PLoS One: PUBLIC LIBRARY SCIENCE, 2014. - Vol. 9, Is. 2. - Ст.e87551. - ISSN 1932-6203, DOI 10.1371/journal.pone.0087551
Примечания : Cited References: 64. - This study was financially supported by Project "Biotechnologies of novel biomaterials: Innovative Biopolymers and Biomedicine Devices" (Agreement No. 11.G34.31.0013 with Amendment No. 1 of 15 February 2013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning." The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Предметные рубрики: RALSTONIA-EUTROPHA
BIODEGRADABLE POLYHYDROXYALKANOATES
AEROMONAS-HYDROPHILA
ESCHERICHIA-COLI
MOLECULAR-WEIGHT
SURFACE-ENERGY
NORTH PACIFIC
TERPOLYESTER
BIOSYNTHESIS
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE-CO-3-HYDROXYHEXANOATE)
Аннотация: This study investigates kinetic and production parameters of a glucose-utilizing bacterial strain, C. eutrophus B-10646, and its ability to synthesize PHA terpolymers. Optimization of a number of parameters of bacterial culture (cell concentration in the inoculum, physiological activity of the inoculum, determined by the initial intracellular polymer content, and glucose concentration in the culture medium during cultivation) provided cell concentrations and PHA yields reaching 110 g/L and 80%, respectively, under two-stage batch culture conditions. Addition of precursor substrates (valerate, hexanoate, propionate, c-butyrolactone) to the culture medium enabled synthesis of PHA terpolymers, P(3HB/3HV/4HB) and P(3HB/3HV/3HHx), with different composition and different molar fractions of 3HB, 3HV, 4HB, and 3HHx. Different types of PHA terpolymers synthesized by C. eutrophus B-10646 were used to prepare films, whose physicochemical and physicalmechanical properties were investigated. The properties of PHA terpolymers were significantly different from those of the P3HB homopolymer: they had much lower degrees of crystallinity and lower melting points and thermal decomposition temperatures, with the difference between these temperatures remaining practically unchanged. Films prepared from all PHA terpolymers had higher mechanical strength and elasticity than P3HB films. In spite of dissimilar surface structures, all films prepared from PHA terpolymers facilitated attachment and proliferation of mouse fibroblast NIH 3T3 cells more effectively than polystyrene and the highly crystalline P3HB.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Semenov D.A., Sudachkova N.E., Khlebopros R.G.
Заглавие : A new scheme of lignin biosynthesis and the mechanism of its regulation of functional properties.
Место публикации : Doklady. Biochemistry and biophysics. - 2002. - Vol. 382. - С. 50-52. - ISSN 16076729 (ISSN)
Ключевые слова (''Своб.индексиров.''): lignin--peroxidase--phenol derivative--polymer--article--biosynthesis--cell wall--chemistry--metabolism--oxidation reduction reaction--physiology--plant--cell wall--lignin--oxidation-reduction--peroxidase--phenols--plants--polymers
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Zhila N., Kiselev E., Shishatskaya E.
Заглавие : A study of synthesis and properties of poly-3-hydroxybutyrate/diethylene glycol copolymers
Место публикации : Biotechnol. Prog.: John Wiley and Sons Inc., 2016. - Vol. 32, Is. 4. - С. 1017-1028. - ISSN 87567938 (ISSN) , DOI 10.1002/btpr.2267
Ключевые слова (''Своб.индексиров.''): biocompatibility--biosynthesis--molecular weight--physicochemical and mechanical properties--poly(3-hydroxybutyrate)/diethylene glycol copolymers--biochemistry--biocompatibility--biosynthesis--cell culture--cell membranes--cells--cytology--fatty acids--glycols--molecular weight--american institute of chemical engineers--biological properties--chemical compositions--cytoplasmic membrane--degree of saturations--physico-chemical and mechanical properties--physiological effects--poly-3-hydroxybutyrate--biomechanics
Аннотация: This study investigates synthesis of poly(3-hydroxybutyrate)/diethylene glycol copolymers (P3HB/DEG) by Cupriavidus eutrophus B-10646 cells as related to DEG concentration in the medium and the time when it is added to the culture of cells synthesizing P3HB. The study determines the limits of physiological effect of DEG on C. eutrophus cells, showing that at DEG concentrations above 30 g/L, it inhibits cell growth, decreasing cell concentration and total P3HB/DEG yield and inducing an increase in the degree of saturation of fatty acids in lipids of cell cytoplasmic membrane. A series of copolymers containing different molar fractions of DEG (between 0.13 and 3.0 mol%) have been synthesized and their physicochemical, physical/mechanical, and biological properties have been investigated as related to the chemical composition and proportions of DEG monomers of the polymers. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1017–1028, 2016. © 2016 American Institute of Chemical Engineers
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Xu S., Wang T., Liu G., Cao Z., Frank L. A., Jiang S., Zhang C., Li Z., Krasitskaya V. V., Li Q., Sha Y., Zhang X., Liu H., Wang J.
Заглавие : Analysis of interactions between proteins and small-molecule drugs by a biosensor based on a graphene field-effect transistor
Место публикации : Sens Actuators, B Chem: Elsevier B.V., 2021. - Vol. 326. - Ст.128991. - ISSN 09254005 (ISSN), DOI 10.1016/j.snb.2020.128991
Аннотация: We synthesized large-area single-crystal graphene sheets to use them in biosensors based on field-effect transistors (FET) for quantitative analysis of interaction kinetics and affinity between the imatinib drug and its target protein kinase Abl1. The G-FET biosensor showed an excellent performance and recognized imatinib at as low as 15.5 fM. The biosensor also showed a linear response to the logarithm of imatinib concentration in the 0.1 pM-10 ?M range. This graphene-based FET biosensor (G-FET) was also applied to quantify Abl1 Y253 F mutation and Abl1 dependency on Mg2+ to bind to imatinib in real-time. Results demonstrated in this work clearly showed that the novel G-FET biosensors are very promising to analyze interactions between proteins and low molecular weight drugs. © 2020 Elsevier B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Dubinnyi M.A., Rodionova N.S., Nadezhdin K.D., Marques S.M., Esteves Da Silva J.C.G., Shimomura O., Yampolsky I.V.
Заглавие : AsLn2, a luciferin-related modified tripeptide from the bioluminescent earthworm Fridericia heliota
Место публикации : Tetrahedron Letters. - 2014. - Vol. 55, Is. 2. - С. 463-465. - ISSN 00404039 (ISSN) , DOI 10.1016/j.tetlet.2013.11.061
Ключевые слова (''Своб.индексиров.''): bioluminescence--fridericia heliota--luciferin--modified peptide
Аннотация: AsLn2, an unusual modified peptide, was isolated from the bioluminescent earthworm Fridericia heliota (Enchytraeidae). Its structure, elucidated by NMR and mass spectrometry, includes residues of tyrosine, CompX (a novel tyrosine modification product, reported in the accompanying paper), and N(omega)-acylated lysine. Chromatography, UV, and 1H NMR data imply a close structural similarity of AsLn2 with F. heliota luciferin. AsLn2 appears to be an intermediate or by-product in F. heliota luciferin biosynthesis. © 2013 Elsevier Ltd. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nesterenko T.V., Tikhomirov A.A., Shikhov V.N., Zavorueva E.N.
Заглавие : Assessment of plant leaf response to long-term exposure at high temperature using the method of thermoinduced chlorophyll fluorescence.
Место публикации : Doklady. Biochemistry and biophysics. - 2001. - Vol. 378. - С. 165-169. - ISSN 16076729 (ISSN)
Ключевые слова (''Своб.индексиров.''): chlorophyll--pigment--article--biosynthesis--chemistry--light--metabolism--plant leaf--plant physiology--radiation exposure--spectrofluorometry--temperature--thermodynamics--time--chlorophyll--light--pigments, biological--plant leaves--plant physiology--spectrometry, fluorescence--temperature--thermodynamics--time factors
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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Kiselev E. G., Zhila N. O., Volova T. G.
Заглавие : Biodegradable polymers - Perspectives and applications in agriculture
Место публикации : IOP Conference Series: Earth and Environmental Science: IOP Publishing Ltd, 2021. - Vol. 689: 2020 International Conference on Germany and Russia: Ecosystems Without Borders, EcoSystConfKlgtu 2020 (5 October 2020 through 10 October 2020, ) Conference code: 167944, Is. 1. - Ст.012036. - , DOI 10.1088/1755-1315/689/1/012036
Аннотация: The paper presents a brief overview of the results of the implementation of the project "Agropreparations of the new generation: a strategy of construction and realization". The first part contains the analysis of the growth of the wild-type strain Cupriavidus necator B-10646 (formerly eutrophus) and the synthesis of polyhydroxyalkanoates by this strain on various substrates: glycerol, palm oil, Siberian oil seed, sunflower seed oils, and oleic acid. On refined glycerin, a highly productive process is implemented when scaling up, allowing to obtain 128 ± 11 g / L PHA. Evaluation of oils has shown that palm oil is the best carbon substrate. The second part presents the results of the development of environmentally friendly slow-release pesticide formulations. They are a degradable matrix of poly-3-hydroxybutyrate mixed with natural materials (peat, clay, wood flour), into which a pesticide (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl, azoxystrobin, epoxiconazole, and tebuconazole) has been. The developed preparations showed high activity against pathogenic fungi and weeds and had a much weaker negative effect on the soil microflora. Studies of the degradation of the developed preparations and the release of pesticides into the soil confirm their effectiveness over a long period of time, up to 90 days. © Published under licence by IOP Publishing Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitel'zon I. I.
Заглавие : Biophysics to Ecology
Колич.характеристики :12 с
Место публикации : Her. Russ. Acad. Sci.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2019. - Vol. 89, Is. 6. - С. 523-534. - ISSN 1019-3316, DOI 10.1134/S1019331619060066. - ISSN 1555-6492(eISSN)
Примечания : Cited References:25
Аннотация: This report presents some new methodological opportunities that biophysics can offer for solving the fundamental problem of planetary ecology-deciphering the mechanism that maintains the equilibrium state of the biosphere. The Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, where the author of this report works, is developing two complementary directions in ecological biophysics. The first is monitoring the vital activity of natural and constructed ecosystems by optical methods using the example of bioluminescence of the sea and closed ecosystems, and the second is studying the laws of parametric biosynthesis control and creating biotechnological control systems for these processes in order to construct noosphere-like ecosystems, in particular, for human life-support and survival systems in extreme conditions on the Earth and in space.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitel’zon I. I.
Заглавие : Biophysics to Ecology
Место публикации : Her. Russ. Acad. Sci.: Pleiades Publishing, 2019. - Vol. 89, Is. 6. - С. 523-534. - ISSN 10193316 (ISSN), DOI 10.1134/S1019331619060066
Аннотация: Abstract: This report presents some new methodological opportunities that biophysics can offer for solving the fundamental problem of planetary ecology—deciphering the mechanism that maintains the equilibrium state of the biosphere. The Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, where the author of this report works, is developing two complementary directions in ecological biophysics. The first is monitoring the vital activity of natural and constructed ecosystems by optical methods using the example of bioluminescence of the sea and closed ecosystems, and the second is studying the laws of parametric biosynthesis control and creating biotechnological control systems for these processes in order to construct noosphere-like ecosystems, in particular, for human life-support and survival systems in extreme conditions on the Earth and in space. © 2019, Pleiades Publishing, Ltd.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Menshikova O., Zhila N., Vasiliev A., Kiselev E., Peterson I., Shishatskaya E., Thomas S.
Заглавие : Biosynthesis and properties of P(3HB-co-3HV-co-3H4MV) produced by using the wild-type strain Cupriavidus eutrophus B-10646
Место публикации : Journal of Chemical Technology and Biotechnology. - 2018. - Article in press. - ISSN 02682575 (ISSN) , DOI 10.1002/jctb.5763
Ключевые слова (''Своб.индексиров.''): 3-hydroxy-4-methylvalerate--physicochemical properties--polyhydroxyalkanoates--synthesis--biochemistry--chemical industry--synthesis (chemical)--3-hydroxy-4-methylvalerate--crystalline-to-amorphous--degree of crystallinity--degrees of crystallinity--physicochemical property--polyhydroxyalkanoates--processing properties--wild-type strain--polymers
Аннотация: BACKGROUND: Polyhydroxyalkanoates (PHA) containing 3-hydroxy-4-methylvalerate (3H4MV) have better processing properties than other PHA types and were not prone to ageing. The main challenge in the production of these polymers is to simultaneously achieve high cell biomass, high polymer content, and high molar fraction of 3H4MV. The aim of this study was to investigate the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxy-4-methylvalerate) [P(3HB-co-3 HV-co-3H4MV)] by Cupriavidus eutrophus B-10646, synthesize polymers with different contents of 3H4MV and investigate their properties. RESULTS: It was shown that 4-methylvalerate (4MV, the precursor substrate) concentration in the culture medium must not be higher than 1 g L?1, and that 4MV must be carefully dosed, with at least 10–12 h intervals between supplementations. A series of polymers with molar fractions of 3H4MV between 2.7 and 11.3 mol% was synthesized. Degrees of crystallinity, molecular weight characteristics, and thermal properties of the polymers have been investigated as dependent on proportions of monomers. CONCLUSION: The content of 3H4MV depends on the concentration of 4MV and conditions of carbon nutrition. The molar fraction of 3H4MV had a strong effect on the crystalline to amorphous region ratio. PHA containing 3H4MV specimens synthesized in this study had a degree of crystallinity of below 50%, which remained unchanged for 2.5 years. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Plotnikov V.F.
Заглавие : Biosynthesis of heteropolymeric polyhydroxyalkanoates by chemolithoautotrophic bacteria
Место публикации : Microbiology. - 1998. - Vol. 67, Is. 4. - С. 420-424. - ISSN 00262617 (ISSN)
Ключевые слова (''Своб.индексиров.''): carboxydobacteria--hydrogen-oxidizing bacteria--polyhydroxyalkanoates
Аннотация: Two chemolithoautotrophs - the hydrogen-oxidizing bacterium Alcaligenes eutrophus and the carboxydobacterium Seliberia carboxydohydrogena - were investigated with respect to their ability to produce heteropolymeric polyhydroxyalkanoates (PHA) during mixotrophic growth on CO2 and medium-chain-length (C6 to C9) alkanoic acids. Hydroxy derivatives of the alkanoic acids were incorporated into PHA synthesized by the bacteria studied in proportions dependent on the time of cultivation. Irrespective of the nature of the acid added to the medium, PHAs were found to contain hydroxybutyrate as the main constituent and also hydroxyvalerate and hydroxyhexanoate, whose maximal incorporation was observed 4 to 6 h after the addition of an alkanoic acid to the medium. During further cultivation, the fractions of hydroxyvalerate and hydroxyhexanoate in PHA decreased, and the fraction of hydroxybutyrate and the total intracellular content of the polymer increased. Three-component polyhydroxyalkanoates with different proportions of hydroxybutyrate, hydroxyvalerate, and hydroxyhexanoate were obtained, and some of their properties were analyzed. В© 1998 MAHK Hayka/Interperiodica Publishing.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Steinbuchel A...
Заглавие : Biosynthesis of multi-component polyhydroxyalkanoates by the bacterium Wautersia eutropha
Место публикации : Macromol. Symp.: WILEY-V C H VERLAG GMBH, 2008. - Vol. 269: 4th European Symposium on Biopolymers (OCT 02-04, 2007, Kusadasi, TURKEY). - С. 1-7. - 7. - ISSN 1022-1360, DOI 10.1002/masy.200850901
Примечания : Cited References: 20
Предметные рубрики: RALSTONIA-EUTROPHA
ALCALIGENES-EUTROPHUS
AEROMONAS-HYDROPHILA
ENHANCED PRODUCTION
PHA SYNTHASE
COPOLYESTERS
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYHEXANOATE)
3-HYDROXYBUTYRATE
ACID
Ключевые слова (''Своб.индексиров.''): autotrophic and mixotrophic growth--multi-component polyhydroxyalkanoates--wautersia eutropha
Аннотация: The study addresses the effect of different conditions of carbon nutrition on synthesis of polyhydroxyalkanoates by the bacterium Wautersia eutropha. In experiments with two wild type strains (H16 and 85786), it has been first found that under mixotrophic growth conditions -CO2+ co-substrate (alkanoic acids) - bacteria can synthesize multi-component PHAs, consisting of short- and medium-chain-length monomers with carbon chains containing 4 to 8 atoms. It has been shown that PHA composition is determined by the type of the co-substrate. Fatty acids with odd number of carbons induce bacteria to synthesize four- and five-component PHAs with hydroxybutyrate, hydroxyvalerate as major monomers and hydroxyhexanoate, hydroxyheptanoate and hydroxyoctanoate as minor, occasionally occurring, ones. Fatty acids with even number of carbons induce synthesis of not only their respective monomers (hydroxyhexanoate and hydroxyoctanoate) but also hydroxyvalerate, making possible synthesis of four-component PHAs, containing hydroxybutyrate and hydroxyhexanoate as major components (up to 18 mol%). A family of short- and medium-chain-length four- and five-component PHAs were synthesized and their physicochemical properties examined.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Steinbuchel A.
Заглавие : Biosynthesis of multi-component polyhydroxyalkanoates by the bacterium Wautersia eutropha
Место публикации : Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - С. 1-7. - ISSN 10221360 (ISSN) , DOI 10.1002/masy.200850901
Ключевые слова (''Своб.индексиров.''): autotrophic and mixotrophic growth--multi-component polyhydroxyalkanoates--wautersia eutropha--bacteriology--biochemical engineering--biochemistry--biopolymers--biotechnology--carboxylic acids--fatty acids--ketones--monomers--polymers--autotrophic and mixotrophic growth--carbon chains--carbon nutritions--even numbers--growth conditions--hydroxybutyrate--hydroxyvalerate--mixotrophic--multi-component polyhydroxyalkanoates--odd numbers--physico-chemical properties--polyhydroxyalkanoates--synthesis of--wautersia eutropha--wild types--acids
Аннотация: The study addresses the effect of different conditions of carbon nutrition on synthesis of polyhydroxyalkanoates by the bacterium Wautersia eutropha. In experiments with two wild type strains (H16 and 65786), it has been first found that under mixotrophic growth conditions - Co2 + co-substrate (alkanoic acids) - bacteria can synthesize multi-component PHAs, consisting of short- and medium-chainlength monomers with carbon chains containing 4 to 8 atoms. It has been shown that PHA composition is determined by the type of the co-substrate. Fatty acids with odd number of carbons induce bacteria to synthesize four- and five-component PHAs with hydroxybutyrate, hydroxyvalerate as major monomers and hydroxyhexanoate, hydroxyheptanoate and hydroxyoctanoate as minor, occasionally occurring, ones. Fatty acids with even number of carbons induce synthesis of not only their respective monomers (hydroxyhexanoate and hydroxyoctanoate) but also hydroxyvalerate, making possible synthesis of four-component PHAs, containing hydroxybutyrate and hydroxyhexanoate as major components (up to 18 mol%). A family of short- and medium-chain-length four- and five-component PHAs were synthesized and their physicochemical properties examined. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Kozhevnikov I.V., Steinbuchel A.
Заглавие : Biosynthesis of multicomponent polyhydroxyalkanoates by Wautersia eutropha
Место публикации : Microbiology. - 2007. - Vol. 76, Is. 6. - С. 704-711. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261707060082
Ключевые слова (''Своб.индексиров.''): autotrophic and mixotrophic growth--multicomponent polyhydroxyalkanoates--wautersia eutropha--bacteria (microorganisms)--cupriavidus necator
Аннотация: The effect of carbon supply on polyhydroxyalkanoate (PHA) synthesis by bacteria Wautersia eutropha was studied. Synthesis of multicomponent PHA composed of short-and long-chain monomers (C4-C8) by two natural strains (H16 and B5786) under mixotrophic conditions (CO2 + alkanoic acids as cosubstrates) was demonstrated for the first time. The PHA composition was shown to be dependent on the cosubstrate type. In the presence of odd fatty acids, four-and five-component polymers were synthesized; hydroxybutyrate, hydroxyvalerate, and hydroxyheptanoate were the major monomers, while hydroxyhexanoate and hydroxyoctanoate were minor. In the presence of even fatty acids, PHA contained not only the corresponding molecules (hydroxyhexanoate and hydroxyoctanoate), but also hydroxyvalerate; synthesis of four-component PHA which contain mainly hydroxybutyrate and hydroxyhexanoate (up to 18 mol %) is therefore possible. A series of four-and five-component PHA was synthesized and their physicochemical characteristics were determined. В© 2007 Pleiades Publishing, Ltd.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N. O., Kalacheva G. S., Fokht V. V., Bubnova S. S., Volova T. G.
Заглавие : Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by cupriavidus necator B-10646 from mixtures of oleic acid and 3-hydroxyvalerate precursors
Место публикации : J. Sib. Fed. Univ. - Biol.: Siberian Federal University, 2020. - Vol. 13, Is. 3. - С. 331-341. - ISSN 19971389 (ISSN), DOI 10.17516/1997-1389-0320
Аннотация: Polyhydroxyalkanoates have attracted much attention as biodegradable alternative to petroleum-based synthetic plastics. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] copolymer is one of the best characterized PHA copolymers because of its high commercial potential. However, commercial use of PHAs has been limited by their high price. One approach to reducing the cost of PHA production is to use inexpensive carbon sources (fatty acids, plant oils, etc.). The aim of this work was to study synthesis of P(3HB-co-3HV) by the Cupriavidus necator B-10646 bacterium grown on oleic acid and different biochemical precursors of 3HV. Bacterial cells were grown for 72 h at 30°C and 200 rpm on an incubator shaker. Salts of propionic or valeric acids were used as precursors of 3HV. The content and the composition of the polymer were determined by gas chromatography of fatty acid methyl esters. Lipids and polymer were extracted from biomass using the method of Folch. The addition of potassium propionate and valerate did not inhibit bacterial growth and polymer synthesis, the cell concentration and polymer content reaching 9.3-9.5 g/L and 80-83%, respectively. The addition of potassium valerate or propionate led to the synthesis of (P(3HB-co-3HV)) copolymer containing 21.2 and 14.3 mol% of 3HV, respectively. The number average molecular weight (Mn) of the polymer synthesized by the bacterium on oleic acid alone was 220 kDa; the polydispersity of the polymer was 3.5. The polymer synthesized in the presence of potassium valerate and propionate was characterized by a lower Mn (156-178 kDa) and a higher polydispersity of the polymer (4.4-4.9). The main fatty acids (FA) of intracellular lipids were oleic (33.26% of the total FA) and palmitic acid (27.48% of the total FA). The addition of potassium propionate or valerate did not cause any significant changes in the composition of the FA of intracellular lipids of the strain studied. This study demonstrates the ability of C. necator B-10646 to synthesize P(3HB-co-3HV) from mixtures of oleic acid and 3HV precursors. The data obtained can be used to develop and implement an economically feasible process of the P(3HB-co-3HV) production. © Siberian Federal University. All rights reserved.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bauer S..., Schott A.K., Illarionova V..., Bacher A..., Huber R..., Fischer M...
Заглавие : Biosynthesis of tetrahydrofolate in plants: Crystal structure of 7,8-dihydroneopterin aldolase from Arabidopsis thaliana reveals a novel adolase class
Колич.характеристики :13 с
Место публикации : J. Mol. Biol.: ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD, 2004. - Vol. 339, Is. 4. - P967-979. - ISSN 0022-2836, DOI 10.1016/j.jmb.2004.04.034
Примечания : Cited References: 66
Предметные рубрики: GTP CYCLOHYDROLASE-I
GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE
6-PYRUVOYL TETRAHYDROPTERIN SYNTHASE
ESCHERICHIA-COLI
DIHYDRONEOPTERIN ALDOLASE
FOLIC-ACID
ENZYMATIC SYNTHESIS
DIHYDROPTEROATE SYNTHASE
REACTION-MECHANISM
3-DIMENSIONAL STRUCTURE
Ключевые слова (''Своб.индексиров.''): tetrahydrofolate biosynthesis--aldolase classes--retroaldol reaction--purin binding--schiff base
Аннотация: Dihydroneopterin aldolase (DHNA) catalyses a retroaldol reaction yielding 6-hydroxymethyl-7,8-dihydropterin, a biosynthetic precursor of the vitamin, tetrahydrofolate. The enzyme is a potential target for antimicrobial and anti-parasite chemotherapy. A gene specifying a dihydroneopterin aldolase from Arabidopsis thaliana was expressed in a recombinant Escherichia coli strain. The recombinant protein was purified to apparent homogeneity and crystallised using polyethylenglycol as the precipitating agent. The crystal structure was solved by X-ray diffraction analysis at 2.2 Angstrom resolution. The enzyme forms a D-4-symmetric homo-octamer. Each polypeptide chain is folded into a single domain comprising an antiparallel four-stranded beta-sheet and two long alpha-helices. Four monomers are arranged in a tetrameric ring, and two of these rings form a hollow cylinder. Well defined purine derivatives are found at all eight topologically equivalent active sites. The subunit fold of the enzyme is related to substructures of dihydroneopterin triphosphate epimerase, GTP cyclohydrolase I, and pyruvoyltetrahydropterin synthase, which are all involved in the biosynthesis of pteridine type cofactors, and to urate oxidase, although some members of that superfamily have no detectable sequence similarity Due to structural and mechanistical differences of DHNA in comparison with class I and class II aldolases, a new aldolase class is proposed. (C) 2004 Elsevier Ltd. All rights reserved.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shatilina Z.M., Gubanov M.V., Zadereev E.S., Pavlichenko V.V., Axenov-Gribanov D.V., Sapozhnikova E.A., Protopopova M.V., Bedulina D.S., Timofeyev M.A.
Заглавие : Comparative study of Thermoresistances' cellular mechanisms in representatives of the Gammarus lacustris Sars populations inhabiting Saline Lake Shira (Republic of Khakassia) and a fresh water body in the Cis-Baikal region
Место публикации : Doklady Biological Sciences. - 2010. - Vol. 434, Is. 1. - С. 359-362. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496610050182
Ключевые слова (''Своб.индексиров.''): antioxidant--catalase--fresh water--heat shock protein 70--reactive oxygen metabolite--sea water--amphipoda--animal--article--biosynthesis--comparative study--ecosystem--heat--metabolism--russian federation--amphipoda--animals--antioxidants--catalase--ecosystem--fresh water--hot temperature--hsp70 heat-shock proteins--reactive oxygen species--seawater--siberia--gammarus--gammarus lacustris
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Berestovskaya N.G., Shaloiko L.A., Gorokhovatsky A.Y., Bondar V.S., Vysotski E.S., Maximov J.E., von Doehren H..., Alakhov Y.B.
Заглавие : Cotranslational formation of active photoprotein obelin in a cell-free translation system: Direct ultrahigh sensitive measure of the translation course
Колич.характеристики :7 с
Место публикации : Anal. Biochem.: ACADEMIC PRESS INC, 1999. - Vol. 268, Is. 1. - С. 72-78. - ISSN 0003-2697, DOI 10.1006/abio.1998.3051
Примечания : Cited References: 22
Предметные рубрики: SEQUENCE-ANALYSIS
MESSENGER-RNA
CA-2+-ACTIVATED PHOTOPROTEIN
LIGHT-EMISSION
AEQUORIN
CDNA
CLONING
EXPRESSION
Аннотация: Translation of apoobelin mRNA in a cell-free wheat germ translation system in the presence of coelenterazine and molecular oxygen results in cotranslational formation of active photoprotein. Active obelin formation is recorded by its luminescence, either direct in the translation mixture in the presence of coelenterazine and calcium ions or in aliquots from the translation mixture. In the second case translation is carried out with coelenterazine and EGTA. Registration of the translation course by luminescence of the synthesized product in both cases allows use of apoobelin mRNA at very low concentrations as an internal marker for immediate measure of protein biosynthesis activity of in vitro translation systems. It is shown that the simultaneous translation of any other mRNA does not affect translation of photoprotein mRNAs under standard conditions. Continuous registration of luminescence in a cuvette of a liquid scintillation counter in photon-counting mode varies the time of signal accumulation in a wide temporal range, thus increasing the numerical values of the recorded signals. Registration of photoprotein luminescence during translation can be used to obtain additional information about the translation process, for example codon reading speed, about protein folding, and about the formation of active proteins on ribosomes. (C) 1999 Academic Press.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Berestovskaya N.G., Shaloiko L.A., Gorokhovatsky A.Y., Bondar V.S., Vysotski E.S., Maximov J.E., von Doehren H..., Alakhov Y.B.
Заглавие : Cotranslational formation of active photoprotein obelin in a cell-free translation system: Direct ultrahigh sensitive measure of the translation course
Колич.характеристики :7 с
Место публикации : Anal. Biochem.: ACADEMIC PRESS INC, 1999. - Vol. 268, Is. 1. - P72-78. - ISSN 0003-2697, DOI 10.1006/abio.1998.3051
Примечания : Cited References: 22
Предметные рубрики: SEQUENCE-ANALYSIS
MESSENGER-RNA
CA-2+-ACTIVATED PHOTOPROTEIN
LIGHT-EMISSION
AEQUORIN
CDNA
CLONING
EXPRESSION
Аннотация: Translation of apoobelin mRNA in a cell-free wheat germ translation system in the presence of coelenterazine and molecular oxygen results in cotranslational formation of active photoprotein. Active obelin formation is recorded by its luminescence, either direct in the translation mixture in the presence of coelenterazine and calcium ions or in aliquots from the translation mixture. In the second case translation is carried out with coelenterazine and EGTA. Registration of the translation course by luminescence of the synthesized product in both cases allows use of apoobelin mRNA at very low concentrations as an internal marker for immediate measure of protein biosynthesis activity of in vitro translation systems. It is shown that the simultaneous translation of any other mRNA does not affect translation of photoprotein mRNAs under standard conditions. Continuous registration of luminescence in a cuvette of a liquid scintillation counter in photon-counting mode varies the time of signal accumulation in a wide temporal range, thus increasing the numerical values of the recorded signals. Registration of photoprotein luminescence during translation can be used to obtain additional information about the translation process, for example codon reading speed, about protein folding, and about the formation of active proteins on ribosomes. (C) 1999 Academic Press.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Eremeev A.V., Gitel'zon I.I., Setkov N.A., Volova T.G.
Заглавие : Cytotoxicity of polyhydroxyalkanoates in animal cell cultures.
Место публикации : Doklady Biological Sciences. - 2000. - Vol. 374, Is. 1-6. - С. 539-542. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): dna--hydroxybutyric acid--poly(3 hydroxybutyrate) co (3 hydroxyvalerate)--poly(3 hydroxybutyric acid)--poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate)--poly-beta-hydroxybutyrate--polyester--rna--thymidine--uridine--animal--article--biosynthesis--cell division--cell strain 3t3--cell survival--cytology--drug effect--liver--liver cell--metabolism--mouse--vascular endothelium--3t3 cells--animals--cell division--cell survival--dna--endothelium, vascular--hepatocytes--hydroxybutyrates--liver--mice--polyesters--rna--thymidine--uridine
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