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Theoretical and experimental decisions in the creation of an artificial ecosystem for human life support in space. / L. V. Kirensky [et al.] // Life sciences and space research. - 1971. - Vol. 9. - P75-80 . - ISSN 0075-9422
Кл.слова (ненормированные):
article -- bacterium -- biotechnology -- Chlorella -- energy metabolism -- human -- instrumentation -- man machine interaction -- metabolism -- microclimate -- space flight -- weightlessness -- Bacteria -- Biotechnology -- Chlorella -- Ecological Systems, Closed -- Energy Metabolism -- Environment, Controlled -- Humans -- Life Support Systems -- Man-Machine Systems -- Space Flight -- Weightlessness
Аннотация: All of man's former space flights were not real ventures into space in the biological sense, as his life was supported with unregenerated earth supplies. The coming stage of space exploration requires man's long existence in the cosmos and on the other planets. This stage of man's activity outside the earth become possible only by creating small man-made ecosystems, permitting the support of his metabolism by the recycling of substances of the terrestrial biosphere. Creation of such systems is a new scientific and technical task. Man-made ecosystems are a new product of man's activity, which have no complete analogy, either in nature, or in technology. Stochastic mechanisms, which stabilize biogeocenosis, cannot be effective in small ecosystems. A technique of parametric control over biosynthesis made it possible to calculate, and put to practice, an ecosystem for man with a cyclic regeneration of the atmosphere, water and, partially, food. The specific bio-technological properties of small man-made ecosystems are being analysed. The possibility of their application for man's excursions into space and for the settlement of other planets is being considered.

Scopus
Держатели документа:
Institute of Physics, Akademgorodok, Krasnoyarsk, USSR. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kirensky, L.V.; Gitelson, I.I.; Terskov, I.A.; Kovrov, B.G.; Lisovsky, G.M.; Okladnikov, Y.N.

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Device for the investigation of maximum growth rate of bacteria / I. I. Gitelson, A. M. Kuznetsov, E. K. Rodicheva // Biotechnology Bioengineering Symposium. - 1974. - Vol. 4, Is. II. - P857 . - ISSN 0572-6565
Кл.слова (ненормированные):
bacterial growth -- bacterium -- berol 185 -- control system -- growth -- microorganism -- theoretical study -- vibrionaceae

Scopus
Держатели документа:
Siberian Branch USSR Acad. Sci., Krasnoyarsk, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Kuznetsov, A.M.; Rodicheva, E.K.

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^a343.31.33^2VINITI
П 31

Печуркин, Н. С.
Популяционные аспекты биотехнологии [Текст] : научное издание / Н. С. Печуркин, А. В. Брильков // Abh. Akad. Wiss. DDR Abt. Math., Naturwiss., Techn. - 1982. - N 2. - С. 197-211 . - ISSN 0138-1059

ГРНТИ

34.31.33

РУБ 343.31.33
Рубрики:
БИОТЕХНОЛОГИЯ
   ПОПУЛЯЦИОННЫЕ АСПЕКТЫ
   МИКРОБИОЛОГИЧЕСКИЕ ПРОЦЕССЫ
   СТАБИЛЬНОСТЬ
   ДЛИТЕЛЬНАЯ ФЕРМЕНТАЦИЯ
   ОБЗОРЫ
   BIOTECHNOLOGY
   MICROBIOLOGICAL PROCESSES
   STABILITY
   REVIEW
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Брильков, А.В.

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Effect of carbon monoxide on metabolism and ultrastructure of carboxydobacteria / T. G. Volova [et al.] // World Journal of Microbiology and Biotechnology. - 1993. - Vol. 9, Is. 2. - P160-163, DOI 10.1007/BF00327827 . - ISSN 0959-3993
Кл.слова (ненормированные):
Carbon monoxide -- carboxydobacteria -- cytochromes -- hydrogenase -- Seliberia carboxydohydrogena
Аннотация: Growth of Seliberia carboxydohydrogena was inhibited by CO at 10 to 40% (v/v), resulting in increased substrate utilization and enhanced synthesis of cytochromes and cyclopropane and saturated fatty acids. The bacteria showed increased formation of new membrane structures, with pronounced folding of their cell walls. В© 1993 Rapid Communications of Oxford Ltd.

Scopus
Держатели документа:
Siberian Branch, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Guseinov, O.A.; Kalacheva, G.S.; Medvedeva, S.E.; Puzyr, A.A.

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MOLECULAR-STRUCTURE AND PHYSICOCHEMICAL PROPERTIES STUDY OF POLYHYDROXYBUTYRATE [Текст] / O. V. FALALEEV [и др.] // Dokl. Akad. Nauk. - 1994. - Vol. 337, Is. 6. - С. 813-817. - Cited References: 13 . - 5. - ISSN 0869-5652

РУБ Multidisciplinary Sciences
Рубрики:
POLY(BETA-HYDROXYBUTYRATE)
BIOTECHNOLOGY

Держатели документа:
LV KIRENSKII INST PHYS,KRASNOYARSK,RUSSIA : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
FALALEEV, O.V.; VOLOVA, T.G.; ZEER, E.P.; VASILEV, A.D.

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Cell-free bioluminescent screening of translation inhibitors [Text] / A. Y. Gorokhovatsky [et al.] // Biotechnol. Appl. Biochem. - 1998. - Vol. 27. - P259-263. - Cited References: 21 . - ISSN 0885-4513

РУБ Biochemistry & Molecular Biology + Biotechnology & Applied Microbiology
Рубрики:
DIPHTHERIA-TOXIN
   MESSENGER-RNA
   SYSTEM
   PROTEINS
Аннотация: The possibility of creating new screening methods with a cell-free translation system has been demonstrated with a quantitative determination of diphtheria toxin and some antibiotics (puromycin, kanamycin and tetracycline) as examples. The approach proposed follows from the ability of various substances to inhibit protein synthesis. We used a wheat-germ cell-free translation system stabilized by freeze-drying in the presence of trehalose with the mRNA of the Ca2+-activated photoprotein obelin as a reporter template. This freeze-dried cell-free translation system allows prolonged storage of the detecting system before it is required, increases the reproducibility of the results and simplifies the application procedure. The obelin mRNA extends the sensitivity of the method owing to the high sensitivity of detection of the synthesized protein.

Держатели документа:
Russian Acad Sci, Branch Inst Bioorgan Chem, Pushchino 142292, Moscow Region, Russia
RAS, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Tech Univ Berlin, Inst Biochem & Mol Biol, D-10587 Berlin, Germany
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gorokhovatsky, A.Y.; Shaloiko, L.A.; Bondar, V.S.; Vysotski, E.S.; Maximov, E.E.; von Doehren, H...; Alakhov, Y.B.

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Study of the molecular structure of polyhydroxybutyrate, a termoplastic and degradable biopolymer [Текст] / T. G. Volova [и др.] // Biofizika. - 2000. - Vol. 45, Is. 3. - С. 445-451. - Cited References: 16 . - 7. - ISSN 0006-3029

РУБ Biophysics
Рубрики:
POLY(BETA-HYDROXYBUTYRATE)
BIOTECHNOLOGY
Кл.слова (ненормированные):
polyhydroxybutyrate -- crystalline and amorphous phases -- molecular structure
Аннотация: The molecular structure of polyhydroxybutyrate from hydrogen-oxidizing bacteria Alcaligenes eutrophus was studied by X-ray diffraction analysis. It was shown that the degree of crystallinity of various samples depends little on the conditions of their preparation and is equal to 0.62-0.76. The molecular structure of solid samples and solution of polyhydroxybutyrate in chloroform were studied by the NMR and EPR methods. The conclusion is made that the molecular structure of polyhydroxybutyrate does not depend on the features of the strain and conditions of carbon nutrition of microorganisms producing polyhydroxybutyrate. Defects induced by gamma -radiation in polyhydroxybutyrate were studied. Free radicals were isolated, and their structure was decoded.

Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Russian Acad Sci, LV Kirensky Phys Inst, Siberian Branch, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Vasiliev, A.D.; Zeer, E.P.; Petrakovskaya, E.A.; Falaleev, O.V.

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Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide [Text] / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Appl. Microbiol. Biotechnol. - 2002. - Vol. 58, Is. 5. - P. 675-678, DOI 10.1007/s00253-002-0941-8. - Cited References: 35 . - ISSN 0175-7598

РУБ Biotechnology & Applied Microbiology
Рубрики:
PSEUDOMONAS-OLEOVORANS
   ALCALIGENES-EUTROPHUS
   COAL
   CULTIVATION
   METABOLISM
   SUBSTRATE
   CULTURE
   CO2
   H-2
   O-2
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxyalkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (beta-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly beta-hydroxybutyrate (more than 99 mol%) with trace amounts of beta-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Applied Microbiology and Biotechnology. - 2002. - Vol. 58, Is. 5. - P675-678, DOI 10.1007/s00253-002-0941-8 . - ISSN 0175-7598
Кл.слова (ненормированные):
3 hydroxybutyric acid -- acetoacetyl coenzyme a reductase -- acetyl coenzyme A acyltransferase -- beta hydroxyvalerate -- butyrate dehydrogenase -- carbon monoxide -- electrolyte -- hydrogen -- oxidoreductase -- poly(3 hydroxybutyric acid) -- poly(3 hydroxybutyric acid)synthase -- polyhydroxyalkanoic acid -- polymer -- unclassified drug -- valeric acid -- bacterium -- article -- autotrophy -- bacterial growth -- bacterial strain -- biomass production -- controlled study -- crystallization -- enzyme activity -- molecular weight -- nonhuman -- synthesis -- temperature -- Wautersia eutropha -- Carbon Monoxide -- Culture Media -- Cupriavidus necator -- Fatty Acids -- Lipids -- Polyesters -- Bacteria (microorganisms) -- Negibacteria -- Ralstonia -- Wautersia eutropha
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxy-alkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO (v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (?-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly ?-hydroxybutyrate (more than 99 mol%) with trace amounts of ?-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Br. Russian Academy of Sci., 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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10.

Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood / V. I. Sevastianov [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - P1029-1042, DOI 10.1163/156856203769231547 . - ISSN 0920-5063
Кл.слова (ненормированные):
?-hydroxy acids -- Endotoxins -- Hemocompatibility -- Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (PHB) -- bacterium lipopolysaccharide -- carbon -- complement -- copolymer -- hydroxyacid -- long chain fatty acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- valeric acid derivative -- adult -- article -- biofilm -- biotechnology -- blood analysis -- blood clotting -- blood compatibility -- cell function -- chemical analysis -- chemical composition -- complement activation -- concentration (parameters) -- controlled study -- gas chromatography -- hemostasis -- human -- human cell -- mass spectrometry -- micromorphology -- nonhuman -- priority journal -- purification -- quantitative analysis -- sampling -- synthesis -- thrombocyte adhesion -- Wautersia eutropha -- Biocompatible Materials -- Blood -- Blood Coagulation Tests -- Chromatography, Gas -- Complement Activation -- Cupriavidus necator -- Fatty Acids -- Humans -- Platelet Adhesiveness -- Polyesters -- Surface Properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

Scopus
Держатели документа:
Inst. of Transplantol. Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Inst. of Biophys. of Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sevastianov, V.I.; Perova, N.V.; Shishatskaya, E.I.; Kalacheva, G.S.; Volova, T.G.

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11.

A closed ecological system as a means of providing high quality of life in an antarctic station and as a model of a life-support system for the martian mission / J. I. Gitelson [et al.] // International Astronautical Federation - 55th International Astronautical Congress 2004. - 2004. - Vol. 3: International Astronautical Federation - 55th International Astronautical Congress 2004 (4 October 2004 through 8 October 2004, Vancouver) Conference code: 69653. - P1428-1435
Кл.слова (ненормированные):
Biotechnology -- Environmental impact -- Martian surface analysis -- Photosynthesis -- Psychophysiology -- Water pollution -- Antarctic station -- Closing technology -- Ecological life support system -- Environmental pollution -- Ecosystems
Аннотация: This closing technology developed for experimental BIOS-3 facility seems to be highly expedient for providing normal life conditions in high-latitude settlements and first of all for life-support of Antarctic stations. Obvious advantages of ecological life-support system based on photosynthesis, in comparison with used now in Antarctic Continent, are: 1) highly effective cascade usage of energy for performance of all functions of life-support; 2) minimization of environmental pollution due to processing human excretions and polluted water; 3) providing crew with fresh vegetable food all-the-year-round; 4) contact with alive plants is powerful source of positive emotions and effective means of psychological relaxation; 5) significant reduction of the transport charges on delivery of foodstuff to Antarctic Continent. First of all, the usage of closed ecosystem can be recommended for high-mountainous station "Concordia". Similar ecosystems can be used in northern high-latitude settlements - Russian North, Canadian Northern territories, Alaska. Finally, the experience of closed ecological life-support system operation in Antarctic Continent can serve as the best range for modeling some aspects of Martian mission - its ecological, biotechnological and psychophysiological problems - since there are no natural conditions on the Earth closer to Mars with respect to severity of a place.

Scopus
Держатели документа:
Institute of Biophysics, SB, RAS, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, J.I.; Bartsev, S.I.; Okhonin, V.A.; Mezhevikin, V.V.

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12.

A database on natural and transgenic luminous microorganisms: BiolumBase / S. E. Medvedeva [et al.] // Microbiology. - 2005. - Vol. 74, Is. 2. - P236-243, DOI 10.1007/s11021-005-0057-0 . - ISSN 0026-2617
Кл.слова (ненормированные):
Bioluminescence -- Database -- Natural and transgenic luminous microorganisms -- Bacteria (microorganisms)
Аннотация: The database BiolumBase was designed for the collection and systematization of available information on microorganisms containing bioluminescent systems; it includes two sections: natural and transgenic luminous microorganisms. By now, logic schemes of these sections have been developed, classification of the objects has been performed, ways of presentation of characteristics and structure of fields for input of information have been elaborated, and the necessary program modules have been developed. The database is filled on the basis of published data and our own experimental results; subsequent linkage of the database to the Internet is envisaged. Users will be able to obtain not only catalogues of strains but also information concerning the properties and functions of the known species of luminous bacteria, the structure, regulatory mechanisms, and application of bioluminescent systems and genetically engineered constructions with lux genes, as well as to find references and to search strains by using any set of attributes. The database will provide information that is of interest for the development of microbial ecology and biotechnology, in particular, for the prediction of biological hazard from the application of transgenic strains. В© 2005 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.; Boyandin, A.N.; Lankin, Yu.P.; Kotov, D.A.; Kargatova, T.V.; Rodicheva, E.K.; Popova, L.Yu.

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13.

Database on natural and transgenic luminous microorganisms: "BIOLUMBASE" / S. E. Medvedeva [и др.] // Mikrobiologiya. - 2005. - Vol. 74, Is. 2. - С. 278-286 . - ISSN 0026-3656
Кл.слова (ненормированные):
Bioluminescence -- Database -- Natural and transgenic luminous microorganisms -- photoprotein -- article -- bacterial gene -- bacterium -- biotechnology -- chemistry -- ecology -- factual database -- genetics -- luminescence -- transgene -- Bacteria -- Biotechnology -- Databases, Factual -- Ecology -- Genes, Bacterial -- Luminescence -- Luminescent Proteins -- Transgenes -- Bacteria (microorganisms)
Аннотация: The database "BiolumBase" is designed for the selection and systematization of available world information on microorganisms containing bioluminescent systems; it includes two sections: "natural" and "transgenic" luminous microorganisms. At present, logic schemes of divisions, classification of the objects, presentation of characteristics, and the inputs of relative information, as well as the necessary program modules including links to the database, are developed. The database is constructed on the basis of published data and our own experimental results; the subsequent linkage of the database to the Internet is envisaged. Users will be able to obtain not only the catalogues of strains but also information concerning the properties and functions of the known species of luminous bacteria, the structure, regulatory mechanisms, and application of bioluminescent systems and genetically engineered constructions with live genes, as well as to find references and to search strains by using any set of attributes. The database will provide information that is of interest for the development of microbial ecology and biotechnology, in particular, for the prediction of biological hazard from the application of transgenic strains.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.; Boyandin, A.N.; Lankin, Yu.P.; Kotov, D.A.; Kargatova, T.V.; Rodicheva, E.K.; Popova, L.Yu.

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14.

BIOLUMBASE - The database of natural and transgenic bioluminescent organisms / S. E. Medvedeva [et al.] // Luminescence. - 2005. - Vol. 20, Is. 2. - P90-96, DOI 10.1002/bio.809 . - ISSN 1522-7235
Кл.слова (ненормированные):
Bioluminescence -- Database -- Luminous bacteria -- lux gene -- Marine -- article -- bacterial strain -- bacterium culture -- bacterium isolation -- bioluminescence -- data base -- gene construct -- medical information -- transgenics -- wide area network -- Bacteria -- Bacterial Proteins -- Databases, Factual -- Ecology -- Luminescence -- Luminescent Proteins -- Marine Biology -- Organisms, Genetically Modified -- Photobacterium -- Transgenes
Аннотация: The Institute of Biophysics SB RAS hosts and maintains a specialized collection of luminous bacteria (CCIBSO 836) containing over 700 strains isolated in various regions of the world's oceans. The culture collection is a source of lux genes and biologically active substances. The wide application of bioluminescence in medicine and ecology has given importance to analys-ing information on the structure and functioning of bioluminescence systems in natural and transgenic microorganisms, as well as on their features that are closely interrelated with bioluminescence. The aims of our BIOLUMBASE database are: gathering information on microorganisms with lux genes, their analysis and free access, and distribution of this data throughout the global network. The database includes two sections, natural and transgenic luminous microorganisms, and is updated by our own experimental results, the published literature and internet resources. For the future, a publicly available internet site for BIOLUMBASE is planned. This will list the strains and provide comprehensive information on the properties and functions of luminous bacteria, the mechanisms of regulation of bioluminescence systems, constructs with lux genes, and applications of bioluminescence in microbiology, ecology, medicine and biotechnology. It is noteworthy that this database will also be useful for evaluation of biological hazards of transgenic strains. Users will be able to carry out bibliographic and strain searches starting from any feature of interest. Copyright В© 2005 John Wiley & Sons, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.; Boyandin, A.; Lankin, Y.; Kotov, D.; Rodicheva, E.; Popova, L.

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15.

Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562 / T. G. Volova [et al.] // Applied Microbiology and Biotechnology. - 2006. - Vol. 73, Is. 3. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
Biochemistry -- Carbon -- Genetic engineering -- Glucose -- Hydrogen -- Polyesters -- Cultural properties -- Glucose-utilizing mutant strain -- Nitrogen deficiency -- Polyhydroxyalkanoate (PHA) -- Bacteria -- 3 hydroxybutyric acid -- carbon dioxide -- fructose -- glucose -- hydrogen -- monomer -- polyhydroxyalkanoic acid -- sugar -- bacterium -- carbon -- comparative study -- energy -- ester -- experimental study -- glucose -- hydrogen -- physicochemical property -- article -- bacterial strain -- bacterium culture -- bacterium mutant -- biomass -- chemical analysis -- Cupriavidus necator -- energy consumption -- genetic analysis -- nonhuman -- synthesis -- Bacteria (microorganisms) -- Cupriavidus necator
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO2, fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is ?-hydroxybutyric acid (more than 99 mol %); the identified minor components are ?-hydroxyvaleric acid (0.25-0.72 mol %) and ?-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose. В© Springer-Verlag 2006.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation
Krasnoyarsk State University, Svobodny av. 79, 660041 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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16.

Physiological-biochemical properties and the ability to synthesize polyhydroxyalkanoates of the glucose-utilizing strain of the hydrogen bacterium Ralstonia eutropha B8562. / T. G. Volova [et al.] // Applied microbiology and biotechnology. - 2006. - Vol. 73, Is. 2. - P429-433, DOI 10.1007/s00253-006-0460-0 . - ISSN 0175-7598
Кл.слова (ненормированные):
beta hydroxyvaleric acid -- beta-hydroxyvaleric acid -- carbon dioxide -- ester -- fatty acid -- fructose -- glucose -- hexanoic acid derivative -- hydrogen -- hydroxyacid -- oxygen -- polymer -- ribosome RNA -- valeric acid derivative -- article -- biotechnology -- chemistry -- metabolism -- methodology -- Wautersia eutropha -- Biotechnology -- Carbon Dioxide -- Cupriavidus necator -- Esters -- Fatty Acids -- Fructose -- Glucose -- Hexanoic Acids -- Hydrogen -- Hydroxy Acids -- Oxygen -- Pentanoic Acids -- Polymers -- RNA, Ribosomal
Аннотация: Physiological-biochemical, genetic, and cultural properties of the glucose-utilizing mutant strain Ralstonia eutropha B8562 have been compared with those of its parent strain R. eutropha B5786. It has been shown that growth characteristics of the strain cultured on glucose as the sole carbon and energy source are comparable with those of the parent strain. Strain B8562 is characterized by high polyhydroxyalkanoate (PHA) yields on different carbon sources (CO(2), fructose, and glucose). PHA accumulation in the strain batch cultured on glucose under nitrogen deficiency reaches 90 %. The major monomer in the PHA is beta-hydroxybutyric acid (more than 99 mol %); the identified minor components are beta-hydroxyvaleric acid (0.25-0.72 mol %) and beta-hydroxyhexanoic acid (0.08-1.5 mol %). The strain is a promising PHA producer on available sugar-containing media with glucose.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Trusova, M.Y.; Kalacheva, G.S.; Kozhevnicov, I.V.

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17.

In vitro justification of using endobiliary stents made of polyhydroxyalkanoates / N. M. Markelova [et al.] // Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - P82-91, DOI 10.1002/masy.200850910 . - ISSN 1022-1360
Кл.слова (ненормированные):
Bioresorbable materials -- Endobiliary stents -- Mechanical jaundice (MJ) -- PHAs -- ABS resins -- Biopolymers -- Biotechnology -- Body fluids -- Experiments -- Free radical polymerization -- Liver -- Polymers -- Prosthetics -- Silicon -- Surgery -- Turbulent flow -- Abdominal cavities -- Bile ducts -- Biliary -- Biological properties -- Bioresorbable materials -- Blood analyses -- Endobiliary stents -- Endoprosthesis -- Experimental groups -- Experimental models -- In vitro -- Inflammatory reactions -- Macroscopic changes -- Mechanical jaundice (MJ) -- Minimally invasive -- Negative controls -- PHAs -- Polyhydroxyalkanoates -- Positive control groups -- Radical surgeries -- Reconstructive surgeries -- Suture materials -- Biological materials
Аннотация: Treatment of patients with mechanical jaundice (MJ) has been one of the topical problems of current medicine. The fraction of patients having MJ of oncologic character that can undergo radical surgery does not exceed 25-30%. A way out of such situation is to use minimally invasive endobiliary interventions, the main type of which is endoprosthesis replacement of bile duct lumens. The aim of the work was to study the biological properties of the experimental models of polymer stents made of PHA for endobiliary prosthetics and to investigate the biological properties of PHA suture material for forming of biliary-enteric anastomoses (cholecystoduodenostomy). Experiments were performed on 20 adult mongrel dogs, weighing 10-12 kg. The animals were divided to three groups: the negative control group (intact animals); the positive control group (the animals with implanted endobiliary silicon stents); and the experimental group (the animals with the PHA stents). The animals were monitored for 100 days. The clinical blood analysis was made before the operation, on day 7, 30, 60 and 100 days. During autopsy the presence of exudate, commissural process in the free abdominal cavity and subhepatic spatium, the appearance of choledoch where the prosthesis was located, as well as the appearance of cholecystoenterostomy, liver and duodenum. We did not found any signs of inflammation, cicatrical changes were estimated in the free abdominal cavity and subhepatic spatium. All implanted PHA stents were at their initial places of implantation. After the end of the experiment inflammatory reaction and anastomositis were absent. Macroscopic changes of liver and duodenum were also not detected. Liver function did not have any pathological deviations. These positive results give grounds to conclude that application of PHA as endobiliary stents in reconstructive surgery of bile passages and as suture material is a promising technology. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.

Scopus
Держатели документа:
Krasnoyarsk State Medical Academy, Partizana Zheleznyaka Street, 1, 660022, Krasnoyarsk, Russian Federation
Institute of Biophysics, SB, RAS, Akademgorodok, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Markelova, N.M.; Shishatskaya, E.I.; Vinnik, Y.S.; Cherdantsev, D.V.; Beletskiy, I.I.; Kuznetsov, M.N.; Zykova, L.D.

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18.

Biomedical investigations of biodegradable PHAs / E. I. Shishatskaya // Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - P65-81, DOI 10.1002/masy.200850909 . - ISSN 1022-1360
Кл.слова (ненормированные):
Biodegradable polymers -- Biomedical investigations -- PHA -- ABS resins -- Biodegradable polymers -- Biopolymers -- Biotechnology -- Bone -- Cell culture -- Endothelial cells -- Fibers -- Functional polymers -- Osteoblasts -- Polymers -- Surgery -- Abdominal surgeries -- Biocompatible -- Biodegradable -- Biomedical -- Biomedical investigations -- Bone defects -- Ectopic bones -- Hepatocytes -- In vitro -- Microparticles -- Oral surgeries -- Osteogenesis -- PHA -- Polyhydroxybutyrate -- Polymer devices -- Ralstonia -- Russian academy of sciences -- Two types -- Polymer films
Аннотация: This work is a review of the results of biomedical studies of polymer devices (films, fibers, microparticles, 30 implants) made from resorbable PHAs synthesized by the bacterium Wautersia (Ralstonia) eutropha 65786, using the technology developed at the Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences. Two types of PHAs - polyhydroxybutyrate (PHB) and a hydroxybutyrate/hydroxyvalerate copolymer (PHB/PHV) - have been proven to be biocompatible in vitro in cultures of fibroblasts, endothelial cells, hepatocytes, and osteoblasts, and in short- and long-duration experiments on animals. Polymer films and membranes have been found to be usable as scaffolds for functioning cells and monofilament suture fibers - for stitching muscular-fascial wounds and in abdominal surgery. Ectopic bone formation assay and experiments with the model of segmental osteotomy showed that 30 PHB and PHB/HA implants can be used for reparative osteogenesis. The paper reports beneficial results of using polymers to repair bone defects in oral surgery. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodnui Av., 69, Krasnoyarsk, 660148, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.

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19.

Database of natural luminescent bacteria / S. E. Medvedeva, D. A. Kotov, E. K. Rodicheva // Contemporary Problems of Ecology. - 2008. - Vol. 1, Is. 1. - P120-126, DOI 10.1007/s12259-008-1014-0 . - ISSN 1995-4255
Аннотация: The database of luminescent bacteria stored in the IBSO collection is one of the metasections of BIOLUMBASE. A logical schema of the metasection "Natural luminescent organisms", classification of entities, and methods of attribute presentation have been developed. The database of luminescent bacteria maintained in the IBSO collection is being widened by findings of the collection staff as well as by information from scientific literature. The expectant contents of the database will be useful for resolving various problems of microbial ecology and biotechnology which deal with luminescent bacteria, luminescent system derived from them, and lux-genes cloned to other organisms. A potential user would be able not only to access cataloged data on strains but also to get information on properties, functions, use, and bibliography and to perform an attribute-match search of a strain. В© 2008 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of the RAS, Akademgorodok 50, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, S.E.; Kotov, D.A.; Rodicheva, E.K.

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20.

Luminous bacteria as producers of polyhydroxyalkanoates / A. Boyandin [et al.] // Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - P17-22, DOI 10.1002/masy.200850904 . - ISSN 1022-1360
Кл.слова (ненормированные):
Luminous bacteria -- Polyhydroxyalkanoates -- Polyhydroxybutyrate -- ABS resins -- Acids -- Bacteriology -- Batch cell culture -- Biological materials -- Biomass -- Biopolymers -- Biotechnology -- Cell culture -- Esters -- Hydrocarbons -- Organic compounds -- Polymers -- Renewable energy resources -- Supramolecular chemistry -- Batch cultures -- Dry cells -- Luminous bacteria -- Micro-organisms -- Photobacterium leiognathi -- Photobacterium phosphoreum -- Polyhydroxyalkanoates -- Polyhydroxybutyrate -- Polymer yields -- Vibrio fischeri -- Bioluminescence
Аннотация: The study addresses the ability of luminous bacteria of different taxa (Photobacterium leiognathi, Photobacterium phosphoreum, Vibrio harveyi, Vibrio fischeri) to synthesize polyesters of hydrocarbon acids (polyhydroxyalkanoates, PHAs) as storage macromolecules. The screened strains widely varied in their PHA productivity. Conditions for attaining high polymer yields (including two- and three-component polymers) in batch culture have been determined. The attained polymer yields reached 40-70% of dry cell biomass. The results suggest a conclusion that luminous microorganisms can be considered as producers of multi-component PHAs. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.

Scopus
Держатели документа:
Institute of Biophysics, SB, RAS, Akademgorodok 50/ 50, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodnyi Av. 79, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Boyandin, A.; Kalacheva, G.S.; Medvedeva, S.; Rodicheva, E.; Volova, T.G.

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