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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Berzhanzkaya L.Y., Gitel'zon I.I., Fish A.M.
Заглавие : Bioluminescence of bacteria: a rhythmic process
Место публикации : BIOPHYSICS. - 1973. - Vol. 18, Is. 2. - С. 293-301. - ISSN 00063509 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterium--computer--in vitro study--methodology--microorganism--theoretical study
Аннотация: New results obtained in the detection of rhythms in the low frequency range in the light signal of a small number of photo bacteria are reported. The luminescence of a small number of bacteria was recorded with an apparatus the sensitivity of which was 12 quanta/pulse. To check the possible rhythms in the luminescence of the bacteria, correlation analysis was made of the interpulse intervals of the detection of the signal with a computer. This revealed a definite rhythm in the bacterial luminescence the fundamental frequency of which lies in the region 8 c/s. Profound modulation of a bioluminescent signal at this and multiple frequencies was demonstrated. The harmonic components found in the spectrum of the signal refute the existing view of the continuous character of bacterial luminescence.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sid'ko A.F., Vasil'yev V.A.
Заглавие : Spectral brightness of inland waters and its correlation with phytoplankton content and hydrooptical characteristics
Место публикации : Hydrobiological Journal. - 1988. - Vol. 24, Is. 5. - С. 64-71. - ISSN 00188166 (ISSN)
Ключевые слова (''Своб.индексиров.''): cyanobacteria--diatom--light attenuation--pyrrhophyte--spectral brightness--ussr, dnieper--ussr, krasnoyarsk reservoir--algae--bacillariophyta--chlorophyta--cyanobacteria--dinophyceae--uncultured cyanobacterium
Аннотация: Spectral brightness coefficients are correlated with indices of vertical attenuation of horizontal irradiance, water transparency, phytoplankton chlorophyll content, and phytoplankton distribution. Data were obtained from Krasnoyarsk Reservoir and the Dnieper reservoirs. When diatoms and pyrrhophytes predominate, the maximum spectral brightness is in the vicinity of 580 nm, but when blue-green algae predominate the maxima are in the vicinity of 550 and 720 nm. -from Authors
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : NESTERENKO T.V., SIDKO F.Y.
Заглавие : QUANTITATIVE DESCRIPTION OF SLOW INDUCTION OF CHLOROPHYLL FLUORESCENCE DURING ONTOGENY OF HIGHER-PLANT LEAVES
Колич.характеристики :6 с
Место публикации : RUSSIAN PLANT PHYSIOLOGY: PLENUM PUBL CORP, 1993. - Vol. 40, Is. 1. - P5-10. - ISSN 1070-3292
Примечания : Cited References: 23
Ключевые слова (''Своб.индексиров.''): cucumis-sativus--leaves--ontogeny--chlorophyll fluorescence
Аннотация: Additional integral and kinetic parameters of induction curves have been introduced on the basis of analysis of currently employed parameters of slow induction of fluorescence (SIF) for a more complete quantitative description of SIF changes during ontogenesis of higher plant leaves. High sensitivity of the additional parameters to the age state of leaf tissue as compared with previously employed parameters is demonstrated on the example of cucumber leaves (Cucumis sativus L.). Analysis of the relationship between the photosynthetic rate and SIF parameters of separate cucumber leaf disks revealed the presence of a high positive correlation between the photosynthetic rate and the greatest possible average decline in fluorescence intensity during the inductive period for mature regions of the leaf and regions starting to senesce (the correlation coeffcient attained values of 0.95), which indicates the possibility of using the given SIF parameter in further elaboration of methods for estimation of chloroplast photosynthetic activity at the level of the higher plant leaf.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GORBAN A.N., MIRKES E.M., POPOVA T.G., SADOVSKY M.G.
Заглавие : THE COMPARATIVE REDUNDANCY OF GENES OF VARIOUS ORGANISMS AND VIRUSES
Колич.характеристики :7 с
Место публикации : Genetika: MEZHDUNARODNAYA KNIGA, 1993. - Vol. 29, Is. 9. - P1413-1419. - ISSN 0016-6758
Примечания : Cited References: 20
Предметные рубрики: CODON
Аннотация: This paper is devoted to the comparative stude of redundancy of genetic texts of various organisms and viruses. To determine the tedundance of a gene, we have introduced the strict measure for that latter. The measure for a text's redundance is the length of restriction of Frequence/Correlation Dictionary of a given genetic text. Frequence/Correlation Dictionary is the ser of all subsequences belang to a given genetic text, accompanied by the frequencies of their occurrence. The restriction length is defined as that one, for which all the subsequences (of that length) are unique. We have found, that genes of human viruses are less redundant, in comparison to those of human genes. Other aspects of a comparative redundance invastigations of the genes are discussed. The problem of the determination of truet intron could be treated by this methodology, as well, as the evolution of genome.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gorban' A.N., Mirkes Ye.M., Popova T.G., Sadovskii M.G.
Заглавие : A new approach to the study of the statistical properties of gene sequences
Место публикации : Biophysics. - 1993. - Vol. 38, Is. 5. - P783-787. - ISSN 00063509 (ISSN)
Аннотация: An essentially new method is proposed for studying the statistical properties of gene sequences (g.s.) based on construction of a frequency-correlation dictionary (f.c.d.) for the g.s. being investigated (the f.c.d. is the set of all subsequences (words) with a length from 1 to N together with the frequencies of their encounter in the g.s. studied). It is shown that a measure of the redundancy of the g.s. is the characteristic length of the word d* starting from which all the words in the g.s. are unique, i.e. have the frequency 1. В© 1994.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GORBAN A.N., POPOVA T.G., SADOVSKII M.G.
Заглавие : REDUNDANCY OF GENETIC TEXTS
Колич.характеристики :8 с
Место публикации : Mol. Biol.: PLENUM PUBL CORP, 1994. - Vol. 28, Is. 2. - P206-213. - ISSN 0026-8933
Примечания : Cited References: 14
Ключевые слова (''Своб.индексиров.''): gene--correlation--redundancy
Аннотация: A new method is proposed for measuring and comparing the redundancy of genetic texts. It is based on compiling for a given text a frequency/correlation dictionary encompassing all the words (subsequences) of length from 1 to N encountered in the text (N is text length); the measure of redundancy is the minimal length at which all words are unique. Preliminary results are reported for different genetic texts.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GORBAN A.N., POPOVA T.G., SADOVSKY M.G.
Заглавие : CORRELATION APPROACH TO COMPARING NUCLEOTIDE-SEQUENCES
Колич.характеристики :11 с
Место публикации : Zhurnal Obshchei Biol.: MEZHDUNARODNAYA KNIGA, 1994. - Vol. 55, Is. 04.05.2013. - P420-430. - ISSN 0044-4596
Примечания : Cited References: 22
Аннотация: A new method of evaluation of similarity between two nucleotide sequences is proposed. It is based on comparing frequency/correlation dictionaries of the sequences under investigation. The dictionary is a set of all strings of various length occurring within the sequences accompanied by their frequencies. The method proposed allows to compare sequences of arbitrary lengths, its advantage is absence of necessity of informal (expert) choice of the best fit. Efficiency of the method is demonstrated by comparison of several human genes and viruses.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : MATVEEV S.V., ILLARIONOV B.A., VYSOTSKI E.S., BONDAR V.S., MARKOVA S.V., ALAKHOV Y.B.
Заглавие : OBELIN MESSENGER-RNA - A NEW TOOL FOR STUDIES OF TRANSLATION IN CELL-FREE SYSTEMS
Колич.характеристики :6 с
Место публикации : Anal. Biochem.: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, 1995. - Vol. 231, Is. 1. - С. 34-39. - ISSN 0003-2697, DOI 10.1006/abio.1995.1499
Примечания : Cited References: 17
Предметные рубрики: MESSENGER-RNA
AEQUORIN
PROTEIN
CLONING
CDNA
Аннотация: Obelin mRNA obtained in vitro with the aid of SP6 RNA polymerase was translated in a wheat germ cell-free system, Only the polypeptide with a molecular mass of about 20 kDa was synthesized. The activation of apoobelin with a synthetic coelenterazine revealed a luminescence activity initiated by calcium. The specific activity was 3.6 +/- 0.4 x 10(15) photons per mg of the in vitro synthesized obelin (k = 6.9 s(-1)). The luminescence of the obelin was in a good correlation with the protein concentration calculated by the incorporation of [C-14]Leu. The determination of the amount of de novo synthesized obelin based on measurement of its luminescence is one-thousand times more sensitive than the approach based on the incorporation of labeled amino acid. Thus, obelin mRNA has some advantages for evaluating the efficiency of cell-free translation when compared with standard methods. (C) 1995 Academic Press, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Kalachova G.S., Guseynova V.E.
Заглавие : Rapid assay of fatty acid composition using a portable high-performance liquid chromatograph for monitoring aquatic ecosystems
Место публикации : Journal of Chromatography A. - 1995. - Vol. 695, Is. 2. - С. 223-228. - ISSN 00219673 (ISSN) , DOI 10.1016/0021-9673(94)01090-2
Ключевые слова (''Своб.индексиров.''): fatty acid--alga--article--culture medium--ecology--high performance liquid chromatography--instrument--methodology--priority journal--ultraviolet spectrophotometry--water analysis
Аннотация: The chromatographic conditions presented allowed the separation of the nitrophenacyl derivatives of standards of eleven free fatty acids (FFA) using a portable high-performance chromatograph, suitable for use aboard a research vessel. A statistically significant linear correlation between UV absorbance and amount of the analytes injected was obtained. The method was tested on FFA from algae cultural media. The method can be used for the ecological monitoring of natural waters.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J.
Заглавие : Properties of recombinant fluorescent proteins from Photobacterium leiognathi and their interaction with luciferase intermediates
Место публикации : Biochemistry. - 1995. - Vol. 34, Is. 10. - С. 3300-3309. - ISSN 00062960 (ISSN)
Ключевые слова (''Своб.индексиров.''): luciferase--recombinant protein--article--ligand binding--nonhuman--priority journal--protein isolation--protein protein interaction--protein stability--vibrionaceae--bacterial proteins--binding sites--carrier proteins--circular dichroism--flavin mononucleotide--fluorescence polarization--genes, bacterial--kinetics--ligands--luciferase--luminescence--molecular sequence data--photobacterium--recombinant proteins--spectrophotometry--support, u.s. gov't, p.h.s.--photobacterium leiognathi--vibrionaceae
Аннотация: Ligand binding and luciferase interaction properties of the recombinant protein corresponding to the lumazine protein gene (EMBL X56534) of Photobacterium leiognathi have been determined by fluorescence dynamics, circular dichroism, gel filtration, and SDS-PAGE. Scatchard analysis of a fluorescence titration shows that the apoprotein possess one binding site, and at 30В°C the KdS (?M) are as follows: 6,7-dimethyl-8-ribityllumazine, 0.26; riboflavin, 0.53; and much more weakly bound FMN, 30. All holoproteins are highly fluorescent and have absorption spectra distinct from each other and from the free ligands. The longest wavelength absorption maxima are, respectively (nm, 2В°C), 420,463, and 458. Ligand binding produces no change in the far-UV circular dichroism; all have mean residual ellipticity at 210 nm of -6500 deg cm2 dmol-1, the same as the native protein. However, in the bioluminescence reaction only the lumazine holoprotein shows a bioluminescence effect. Fluorescence emission anisotropy decay was used to establish that none of these holoproteins complexed with native luciferase and that the lumazine protein alone formed a 1:1 complex with the luciferase hydroxyflavin fluorescent transient and the luciferase peroxyflavin intermediates, revealed by a dominant channel of anisotropy loss, with rotational correlation time of 2.5 ns, and attributed to excitation transfer from the luciferase flavin donor to the acceptor, the lumazine ligand. The complex stability was sufficient to allow its isolation by FPLC gel filtration and verification by SDS-PAGE. These methods also confirmed the absence of interaction of the holoflavoproteins.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shchur L.A., Aponasenko A.D., Lopatin V.N., Filimonov V.S., Shepelevich N.V.
Заглавие : Determination of the bacterial concentration in freshwater reservoirs and streams by fluorometry
Колич.характеристики :3 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1997. - Vol. 66, Is. 6. - P726-728. - ISSN 0026-2617
Примечания : Cited References: 8
Ключевые слова (''Своб.индексиров.''): concentration and biomass of bacterioplankton--fluorescence--regression equation
Аннотация: A simple fluorometric method is proposed for rapid determination of the concentration or biomass of bacterioplankton in various natural freshwater reservoirs and streams directly under field conditions. Fluorescamine, which specifically binds to proteins and amino acids in microbial cells, was used as the fluorescent marker. Regression equations were calculated on the basis of the results obtained. The coefficient of correlation between the fluorescence intensity and the bacterial biomass (cell number) was found to be 0.93 (0.89) for the total body of data obtained in the reservoirs studied.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zavoruev V.V., Zavorueva E.N., Shelegov A.V.
Заглавие : Fluorescence induced by light of 380-540 nm in leaves of cucumber depening on the vegetation time and irradiation regime
Колич.характеристики :8 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2000. - Vol. 45, Is. 4. - P704-711. - ISSN 0006-3029
Примечания : Cited References: 25
Предметные рубрики: CHLOROPHYLL
INDUCTION
RATIO
INCREASE
Ключевые слова (''Своб.индексиров.''): slow fluorescence induction--cucumber leaves--vegetation time spectral conditions
Аннотация: The slow fluorescence induction produced iii cucumber leaves by light in the range of wavelengths 380-540 nm and intensity of 180 W/m(2) was studied. The ratio of fluorescence maxima in the red region (F(734)/F(682)) in young and mature leaves was approximately 2. It is assumed that this value depends on an increase in the contribution of the long-wavelength fluorescence due to the spillover effect. In plants grown under natural conditions, the paramster F(734)/F(682) correlated with the concentrations of chlorophyll and carotenoids. In plants grown hi the light of red and blue regions no such correlation was observed and the F(734)/F(682) remained unchanged. It is concluded that the F(534)/F(682) is affected by the intensity and spectral composition of exciting light used during the growing.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Gribovskaya I.V., Moskvicheva A.V., Muchkina E.Y., Chuprov S.M., Ivanova E.A.
Заглавие : Content of metals in compartments of ecosystem of a Siberian pond
Место публикации : Archives of Environmental Contamination and Toxicology. - 2001. - Vol. 41, Is. 2. - С. 157-162. - ISSN 00904341 (ISSN) , DOI 10.1007/s002440010233
Ключевые слова (''Своб.индексиров.''): aluminum--cadmium--calcium--chromium--copper--heavy metal--iron--lead--magnesium--manganese--nickel--potassium--sodium--zinc--aquatic ecosystem--biological uptake--heavy metal--pond--article--bioaccumulation--ecosystem--fish--nonhuman--pond--priority journal--russian federation--sediment--soil pollution--water contamination--animals--ecosystem--environmental monitoring--fishes--geologic sediments--invertebrates--metals, heavy--plants--water pollutants--russian federation
Аннотация: During three field seasons (June-September) of 1997-99 contents of Na, K, Ca, Mg, Fe, Mn, Zn, Cu, Al, Cr, Ni, Cd, and Pb were determined in compartments of ecosystem (surrounding soils, bottom sediments, water, zoobenthos, macrophytes, and fish) of a fish and recreation pond situated at the edge of Krasnoyarsk City (Siberia, Russia). Contents of most parts of metals in soils, water, and macrophytes significantly correlated with each other. As concluded, their contents were determined by natural, general, geochemical peculiarities of the region. Heavy metals, contents of which were higher than federal upper limits of concentration, were revealed. In muscles of fish with different feeding spectra - crucian and perch - concentrations of some metals differed significantly; correlation graphs for metals also had different structures. Comparison of our data with those on diverse aquatic ecosystems of Siberia, Europe, North America, and China published in the last decade was carried out. It was concluded that a distribution of heavy metals in the compartments of an aquatic ecosystem presently have to be determined for each particular water body until general regularities are discovered.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Gribovskaya I.V., Moskvicheva A.V., Muchkina E.Y., Chuprov S.M., Ivanova E.A.
Заглавие : Content of metals in compartments of ecosystem of a Siberian pond
Колич.характеристики :6 с
Место публикации : Arch. Environ. Contam. Toxicol.: SPRINGER-VERLAG, 2001. - Vol. 41, Is. 2. - P157-162. - ISSN 0090-4341
Примечания : Cited References: 26
Предметные рубрики: CHIRONOMUS-RIPARIUS MEIGEN
NELSON RIVER SYSTEM
AQUATIC MACROPHYTES
LARVAE DIPTERA
HEAVY-METAL
COPPER
LEAD
SEDIMENTS
CADMIUM
MANITOBA
Аннотация: During three field seasons (June-September) of 1997-99 contents of Na, K, Ca, Mg, Fe, Mn, Zn, Cu, Al, Cr, Ni, Cd, and Pb were determined in compartments of ecosystem (surrounding soils, bottom sediments, water, zoobenthos, macrophytes, and fish) of a fish and recreation pond situated at the edge of Krasnoyarsk City (Siberia, Russia). Contents of most parts of metals in soils, water, and macrophytes significantly correlated with each other. As concluded, their contents were determined by natural, general, geochemical peculiarities of the region. Heavy metals, contents of which were higher than federal upper limits of concentration, were revealed. In muscles of fish with different feeding spectra-crucian and perch-concentrations of some metals differed significantly; correlation graphs for metals also had different structures. Comparison of our data with those on diverse aquatic ecosystems of Siberia, Europe, North America, and China published in the last decade was carried out. It was concluded that a distribution of heavy metals in the compartments of an aquatic ecosystem presently have to be determined for each particular water body until general regularities are discovered.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gerasimova M.A., Kudryasheva N.S.
Заглавие : Effects of potassium halides on bacterial bioluminescence
Колич.характеристики :5 с
Место публикации : J. Photochem. Photobiol. B-Biol.: ELSEVIER SCIENCE SA, 2002. - Vol. 66, Is. 3. - P218-222. - ISSN 1011-1344, DOI 10.1016/S1011-1344(02)00240-3
Примечания : Cited References: 20
Предметные рубрики: COMPONENTS
SEWAGE
Ключевые слова (''Своб.индексиров.''): bioluminescence--halides--heavy atom--inhibition--activation
Аннотация: The effects of potassium halides KCl, KBr and KI on NADH:FMN-oxidoreductase-luciferase bioluminescent coupled enzyme system were studied. The influence of salt additions on bioluminescence intensity and bioluminescence light yield was investigated. The inhibition and activation parameters of the salts were calculated using their dependencies on concentration of the salts. The correlation between the inhibition of bioluminescence intensity and the halide mass was demonstrated: the inhibiting ability of the salts increases with the increase of atomic weight of the anions. The inhibition parameters increase and the activation parameters decrease, accordingly. (C) 2002 Elsevier Science B.V. All rights reserved.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Nemtseva E.V., Visser AJWG, van Hoek A...
Заглавие : Interaction of aromatic compounds with Photobacterium leiognathi luciferase: fluorescence anisotropy study
Колич.характеристики :6 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 2003. - Vol. 18, Is. 3. - P156-161. - ISSN 1522-7235, DOI 10.1002/bio.719
Примечания : Cited References: 25
Предметные рубрики: ELECTRON-EXCITED-STATES
BACTERIAL BIOLUMINESCENCE
LUMAZINE PROTEIN
MECHANISM
Ключевые слова (''Своб.индексиров.''): bioluminescence--luciferase--fluorescent compounds--anisotropy decay
Аннотация: The time-resolved and steady-state fluorescence techniques,were employed to elucidate possible interactions of four aromatic compounds (anthracene, POPOP, MSB and 1,4-naphthalendiol) with bacterial luciferase. Fluorescence spectra and fluorescence anisotropy decays of these compounds were studied in ethanol, water-ethanol solutions and in the presence of bacterial luciferase. Shifts of fluorescent spectra and differences in rotational correlation times are interpreted in terms of weak (hydrophobic) interactions of the molecules with the enzyme. These interactions suggest the feasibility of intermolecular energy transfer by an exchange resonance mechanism with a collision-interaction radius as a way of excitation of these compounds in the reaction catalysed by bacterial luciferase. Copyright (C) 2003 John Wiley Sons, Ltd.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Kalachova G.S., Kravchuk E.S., Dubovskaya O.P., Ivanova E.A.
Заглавие : Particulate fatty acids in two small Siberian reservoirs dominated by different groups of phytoplankton
Колич.характеристики :10 с
Место публикации : Freshw. Biol.: BLACKWELL PUBLISHING LTD, 2003. - Vol. 48, Is. 3. - P394-403. - ISSN 0046-5070, DOI 10.1046/j.1365-2427.2003.01009.x
Примечания : Cited References: 44
Предметные рубрики: ENCLOSED EXPERIMENTAL ECOSYSTEM
BLUE-GREEN-ALGAE
DAPHNIA-GALEATA
FOOD QUALITY
MICROALGAE
ZOOPLANKTON
NUTRITION
GROWTH
LAKE
REPRODUCTION
Ключевые слова (''Своб.индексиров.''): cyanobacteria--daphnids--diatoms--fatty acids
Аннотация: 1. We studied the composition of fatty acids (FAs) in the seston from two small freshwater reservoirs (Bugach and Lesnoi) with distinct periodicity of domination by cyanobacteria and eukaryotic algae during the growth season. 2. The diatoms in the both reservoirs were characterised by a high content of 14:0 and C16 unsaturated acids, whereas that of the essential FA 20:5omega3 [eicosapentanoic acid (EPA)] was low. The correlation between this polyunsaturated FA (PUFA) and diatom biomass was not significant in either reservoir. The percentage of 20:5omega3 in seston significantly correlated with the biomass of euglenophyta in Bugach and dinophyta in Lesnoi. Hence the diatoms, usually referred as a valuable food for zooplankton, were not an important source of the essential PUFA in these systems. 3. The dominant cyanobacteria in Bugach, and the green algae in Lesnoi, both contained the same marker acids: 18:3omega3 and 18:2omega6. Hence, a discrimination between these two phytoplanktonic groups on the basis of FA biomarkers may be difficult in some cases. 4. We found no significant correlation between the content of 20:5omega3 in seston and the biomass of the dominant daphniids in either reservoir. This is contrary to expectations, based on the literature, that EPA is generally important. Rather, the biomass of the two dominant Daphnia species in Bugach correlated strongly with the content of 18:3omega3 in the seston. The cyanobacteria were a probable source of this omega3 FA for Daphnia . We conclude that EPA is not always important for Daphnia populations although, in such cases, some other PUFA (e.g. 18:3omega3) might be related to their growth.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Remmel N.N., Kratasyuk V.A., Maznyak O.M., Inzhevatkin E.V., Nefedov V.P.
Заглавие : Bioluminescent analysis of intensity of pathological oxidative processes in cells of perfused rat liver after hyperthermia
Колич.характеристики :3 с
Место публикации : Bull. Exp. Biol. Med.: CONSULTANTS BUREAU, 2003. - Vol. 135, Is. 1. - P43-45. - ISSN 0007-4888, DOI 10.1023/A:1023489727703
Примечания : Cited References: 15
Ключевые слова (''Своб.индексиров.''): bioluminescent analysis--controlled perfusion of isolated liver--hyperthermia--oxidative stress
Аннотация: We studied the possibility of evaluation of the intensity of pathological oxidative processes in rat liver using an integral bioluminescent test with controlled perfusion of the isolated organ. The test revealed a significant correlation between the level of TBA-reactive products and bioluminescence intensity.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Kalachova G.S., Kravchuk E.S., Dubovskaya O.P., Ivanova E.A.
Заглавие : Particulate fatty acids in two small Siberian reservoirs dominated by different groups of phytoplankton
Место публикации : Freshwater Biology. - 2003. - Vol. 48, Is. 3. - С. 394-403. - ISSN 00465070 (ISSN) , DOI 10.1046/j.1365-2427.2003.01009.x
Ключевые слова (''Своб.индексиров.''): cyanobacteria--daphnids--diatoms--fatty acids--alga--cyanobacterium--fatty acid--periodicity--phytoplankton--reservoir--seston--algae--bacillariophyta--chlorophyta--cyanobacteria--daphnia--dinophyceae--euglenida--eukaryota
Аннотация: 1. We studied the composition of fatty acids (FAs) in the seston from two small freshwater reservoirs (Bugach and Lesnoi) with distinct periodicity of domination by cyanobacteria and eukaryotic algae during the growth season. 2. The diatoms in the both reservoirs were characterised by a high content of 14:0 and C16 unsaturated acids, whereas that of the essential FA 20:5?3 [eicosapentanoic acid (EPA)] was low. The correlation between this polyunsaturated FA (PUFA) and diatom biomass was not significant in either reservoir. The percentage of 20:5?3 in seston significantly correlated with the biomass of euglenophyta in Bugach and dinophyta in Lesnoi. Hence the diatoms, usually referred as a valuable food for zooplankton, were not an important source of the essential PUFA in these systems. 3. The dominant cyanobacteria in Bugach, and the green algae in Lesnoi, both contained the same marker acids: 18:3?3 and 18:2?6. Hence, a discrimination between these two phytoplanktonic groups on the basis of FA biomarkers may be difficult in some cases. 4. We found no significant correlation between the content of 20:5?3 in seston and the biomass of the dominant daphniids in either reservoir. This is contrary to expectations, based on the literature, that EPA is generally important. Rather, the biomass of the two dominant Daphnia species in Bugach correlated strongly with the content of 18:3?3 in the seston. The cyanobacteria were a probable source of this ?3 FA for Daphnia. We conclude that EPA is not always important for Daphnia populations although, in such cases, some other PUFA (e.g. 18:3?3) might be related to their growth.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Shishatskaya E., Sevastianov V., Efremov S., Mogilnaya O.
Заглавие : Results of biomedical investigations of PHB and PHB/PHV fibers
Место публикации : Biochemical Engineering Journal. - 2003. - Vol. 16, Is. 2. - С. 125-133. - ISSN 1369703X (ISSN) , DOI 10.1016/S1369-703X(03)00038-X
Ключевые слова (''Своб.индексиров.''): biocompatibility--fibers--implants--poly-3-hydroxybutyrate (phb)--poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (phb/phv)--polyhydroxyalkanoates (phas)--tissue reaction--bacteriology--calcification (biochemistry)--copolymers--flammability--tissue--tissue response--biomedical engineering--acid phosphatase--poly(3 hydroxybutyric acid)--poly(3 hydroxybutyric acid) co poly(3 hydroxyvaleric acid)--polyhydroxyalkanoic acid--suture material--unclassified drug--animal experiment--article--bacterial metabolism--biochemistry--biocompatibility--biomedicine--calcification--catgut--correlation analysis--dose time effect relation--enzyme activity--fascia--fiber--foreign body--functional assessment--giant cell--implantation--in vivo study--inflammation--intermethod comparison--macrophage--materials testing--multinuclear cell--muscle injury--necrosis--nonhuman--parameter--phagocytosis--physiology--postoperative period--priority journal--process monitoring--ralstonia eutropha b 5786--scar formation--silk--strength--suture--synthesis--tissue reaction--wautersia eutropha--wound healing--animalia--ralstonia--wautersia eutropha
Аннотация: The paper presents the results of biomedical investigations of sutures made of polyhydroxyalkanoates (PHAs) of two types (poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV)), synthesized by the bacteria Ralstonia eutropha B 5786 in the experiment on test animals in vivo, in comparison with silk and catgut sutures. PHB and PHB/PHV (PHBV) implants produced no adverse effect on physiological, biochemical and functional parameters of the animals during the post-surgery period. The tested PHA sutures featured the necessary strength throughout the healing period of the muscle-fascial cuts. The reaction of tissues to the implantation of PHB and PHB/PHV fibers fitted into the usual scheme characteristic of the wound process and of the reaction to a foreign-body invasion. This reaction and the reaction of tissues to silk had the similar nature and period of inflammation, but it was much less pronounced than the reaction to catgut. The tissue response to the implantation of PHAs consisted in a short-duration (up to 2 weeks) post-traumatic inflammation and the formation of a fibrous capsule less than 200 ?m thick during weeks 4-8, which in 4-6 months was reduced to 40-60 ?m in the course of reverse development. There were no adverse changes, such as suppurative inflammation, necrosis, calcification, and malignization of the cicatrice, at the site of implantation of PHA filaments, unlike in the cases with silk and catgut. In the case of PHA implantation there was a typical prolonged (throughout the post-surgery monitoring period) pronounced macrophagal stage with a large number of macrophages present. The macrophages were of the phagocytic type and multinucleate giant foreign cells, with a high activity of acid phosphomonoesterase that correlated with the activity of the enzyme in blood. Throughout the period of monitoring no differences in the tissue response to the implantation of the polymer filaments of two PHA types were recorded. В© 2003 Elsevier Science B.V. All rights reserved.
Scopus
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