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1.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kozhevnikov I.V., Volova T.G., Hai T., Steinbuchel A.
Заглавие : Cloning and molecular organization of the polyhydroxyalkanoic acid synthase gene (phaC) of Ralstonia eutropha strain B5786
Место публикации : Applied Biochemistry and Microbiology. - 2010. - Vol. 46, Is. 2. - С. 140-147. - ISSN 00036838 (ISSN) , DOI 10.1134/S0003683810020031
Ключевые слова (''Своб.индексиров.''): aeromonas punctata--cupriavidus necator--ectothiorhodospira shaposhnikovii--pseudomonas--pseudomonas sp. 61-3--rhodococcus--rhodococcus ruber--rhodospirillum rubrum--thiococcus pfennigii
Аннотация: Class I polyhydroxyalkanoic acid (PHA) synthase gene (phaC) of Ralstonia eutropha strain B5786 was cloned and characterized. R. eutropha B5786 features the ability to synthesize multicomponent PHAs with short- and medium-chain-length monomers from simple carbohydrate substrate. A correlation was made between the molecular structure of PHA synthase and substrate specificity and the ability of strain-producers to accumulate PHAs of this or that structure. A strong similarity of PHA synthase of R. eutropha strain B5786 with PHA synthase of R. eutropha strain H16, which, as opposed to strain B5786, enables to incorporate medium chain length PHAs if hexanoate is used as carbon source, exhibited 99%. A correlation between the structure of PHA synthase of B5786 strain with synthases of microorganisms which synthesize short and medium chain length PHAs similarly to B5786 strain, showed an identity level from 26 to 41% (homology with synthase of Rhodospirillum rubrum makes 41%, Ectothiorhodospira shaposhnikovii makes 26%, Aeromonas punctata makes 40%, Thiococcus pfennigii makes 28%, Rhodococcus ruber makes 38%, and with PhaCl and PhaC2 synthases of Pseudomonas sp. 61-3 makes 34 and 37%, respectively). This allows for speaking about the absence of a direct connection between the molecular organization of PHA synthases and their functional abilities, namely, the ability to synthesize PHAs of a particular composition. В© 2010 Pleiades Publishing, Ltd.
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2.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Plotnikov V.F., Shishatskaya E.I., Mironov P.V., Vasil'ev A.D.
Заглавие : Physicochemical properties of two-component polyhydroxyalkanoates, 3-hydroxybutyrate-3-hydroxyvalerate copolymers
Место публикации : Biophysics. - 2004. - Vol. 49, Is. 6. - С. 934-942. - ISSN 00063509 (ISSN)
Ключевые слова (''Своб.индексиров.''): hydroxybutyrate-hydroxyvalerate copolymers--physicochemical properties--polyhydroxyalkanoates--structure--bacteria (microorganisms)--cupriavidus necator
Аннотация: A series of two-component polyhydroxyalkanoates composed of hydroxybutyrate-hydroxyvalerate copolymers with different monomer ratio was obtained with the use of bacteria Ralstonia eutropha B5786. The properties of the polyhydroxyalkanoates in comparison with the homopolymer of hydroxybutyric acid were studied by X-ray diffraction analysis, IR spectroscopy, differential thermal analysis, and viscometry. The ratio of crystalline to amorphous phase in the copolymers tends to unity with increasing hydroxyvalerate content. This is accompanied by a decrease in the degree of crystallinity of the copolymers from 70-80 to 45-50%, the dependence is virtually linear within the range, of hydroxyvalerate mole fraction from several to 25-30 mol%. Thermal characteristics, melting temperature (Tm) and decomposition temperature (Td), of the polyhydroxyalkanoate copolymers are lower than those for polyhydroxybutyrate, whose Tm and Td are 168-170 and 260-265В°C, respectively. Both parameters decrease to 150-160 and 200-220В°C, respectively, when the hydroxyvalerate mole fraction is raised. No distinct correlation between polymer composition and molecular weight has been revealed. Copyright В© 2004 by MAIK "Nauka/Interperiodica".
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3.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Shishatskaya E., Sevastianov V., Efremov S., Mogilnaya O.
Заглавие : Results of biomedical investigations of PHB and PHB/PHV fibers
Место публикации : Biochemical Engineering Journal. - 2003. - Vol. 16, Is. 2. - С. 125-133. - ISSN 1369703X (ISSN) , DOI 10.1016/S1369-703X(03)00038-X
Ключевые слова (''Своб.индексиров.''): biocompatibility--fibers--implants--poly-3-hydroxybutyrate (phb)--poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (phb/phv)--polyhydroxyalkanoates (phas)--tissue reaction--bacteriology--calcification (biochemistry)--copolymers--flammability--tissue--tissue response--biomedical engineering--acid phosphatase--poly(3 hydroxybutyric acid)--poly(3 hydroxybutyric acid) co poly(3 hydroxyvaleric acid)--polyhydroxyalkanoic acid--suture material--unclassified drug--animal experiment--article--bacterial metabolism--biochemistry--biocompatibility--biomedicine--calcification--catgut--correlation analysis--dose time effect relation--enzyme activity--fascia--fiber--foreign body--functional assessment--giant cell--implantation--in vivo study--inflammation--intermethod comparison--macrophage--materials testing--multinuclear cell--muscle injury--necrosis--nonhuman--parameter--phagocytosis--physiology--postoperative period--priority journal--process monitoring--ralstonia eutropha b 5786--scar formation--silk--strength--suture--synthesis--tissue reaction--wautersia eutropha--wound healing--animalia--ralstonia--wautersia eutropha
Аннотация: The paper presents the results of biomedical investigations of sutures made of polyhydroxyalkanoates (PHAs) of two types (poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV)), synthesized by the bacteria Ralstonia eutropha B 5786 in the experiment on test animals in vivo, in comparison with silk and catgut sutures. PHB and PHB/PHV (PHBV) implants produced no adverse effect on physiological, biochemical and functional parameters of the animals during the post-surgery period. The tested PHA sutures featured the necessary strength throughout the healing period of the muscle-fascial cuts. The reaction of tissues to the implantation of PHB and PHB/PHV fibers fitted into the usual scheme characteristic of the wound process and of the reaction to a foreign-body invasion. This reaction and the reaction of tissues to silk had the similar nature and period of inflammation, but it was much less pronounced than the reaction to catgut. The tissue response to the implantation of PHAs consisted in a short-duration (up to 2 weeks) post-traumatic inflammation and the formation of a fibrous capsule less than 200 ?m thick during weeks 4-8, which in 4-6 months was reduced to 40-60 ?m in the course of reverse development. There were no adverse changes, such as suppurative inflammation, necrosis, calcification, and malignization of the cicatrice, at the site of implantation of PHA filaments, unlike in the cases with silk and catgut. In the case of PHA implantation there was a typical prolonged (throughout the post-surgery monitoring period) pronounced macrophagal stage with a large number of macrophages present. The macrophages were of the phagocytic type and multinucleate giant foreign cells, with a high activity of acid phosphomonoesterase that correlated with the activity of the enzyme in blood. Throughout the period of monitoring no differences in the tissue response to the implantation of the polymer filaments of two PHA types were recorded. В© 2003 Elsevier Science B.V. All rights reserved.
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4.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Semenchenko V.P., Dubovskaya O.P., Fefilova E.B., Makhutova O.N., Buseva Z.F., Sushchik N.N., Razlutskij V.I., Lepskaya E.V., Baturina M.A., Kalachova G.S., Kononova O.N.
Заглавие : Effect of temperature on contents of essential highly unsaturated fatty acids in freshwater zooplankton
Место публикации : Limnologica. - 2011. - Vol. 41, Is. 4. - С. 339-347. - ISSN 00759511 (ISSN) , DOI 10.1016/j.limno.2011.03.001
Ключевые слова (''Своб.индексиров.''): cladocerans--copepods--essential polyunsaturated fatty acids--freshwater zooplankton--water temperature--biomass--climate effect--community structure--fatty acid--freshwater ecosystem--gas chromatography--lake ecosystem--multivariate analysis--seston--taxonomy--temperature effect--trophic level--zooplankton--animalia--copepoda--crustacea
Аннотация: In 11 lakes from cold and warm regions we studied the content of highly unsaturated fatty acids (HUFA) in seston (130?m) and crustacean zooplankton using gas chromatography-mass spectrometry. An increase of temperature correlated with a decrease of HUFA content in zooplankton. A multivariate canonical correlation analyses revealed, that the decrease of HUFA content was related with a decrease of per cent of copepods in zooplankton communities, which are known to have higher HUFA levels in their biomass, than cladocerans. This means that temperature primarily affected the HUFA levels indirectly, via changing of taxonomic structure of zooplankton community, while the homeoviscous adaptation of zooplankton individuals had comparatively lower importance. As found, water temperature was better predictor of HUFA contents of zooplankton, than the fatty acid composition of seston. Thus, it can be predicted, that a probable climate warming will decrease the content of the essential HUFA in freshwater zooplankton with possible negative consequences for animals of higher trophic levels. В© 2011 Elsevier GmbH.
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5.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Makhutova O.N., Dubovskaya O.P., Kravchuk E.S., Kalachova G.S., Khromechek E.B.
Заглавие : Correlations between fatty acid composition of seston and zooplankton and effects of environmental parameters in a eutrophic Siberian reservoir
Место публикации : Limnologica. - 2010. - Vol. 40, Is. 4. - С. 343-357. - ISSN 00759511 (ISSN) , DOI 10.1016/j.limno.2009.12.004
Ключевые слова (''Своб.индексиров.''): eicosapentaenoic acid--fatty acids--phytoplankton--temperature--zooplankton--canonical analysis--correlation--eutrophic environment--fatty acid--multivariate analysis--phytoplankton--seasonal variation--seston--taxonomy--temperature effect--water temperature--zooplankton--algae--bacillariophyta--ciliophora--cyanobacteria--cyclopoida--rotifera
Аннотация: During two sampling seasons we analyzed on weekly basis fatty acid (FA) composition of seston fraction 130?m and zooplankton fraction 130?m, and compared them using a multivariate canonical correlation analysis (CCA). Besides, we evaluated a possible impact of water temperature and inorganic nutrients on FA composition of the seston and the zooplankton.In spite of significant differences in percentages of several individual FAs, we found very strong canonical correlation (cross-correlation, 1-week lag) between FA composition of the seston and the zooplankton. The most important factor, providing the overall canonical cross-correlation between FA profiles of the seston and the zooplankton fractions was eicosapentaenoic acid (20:5?3, EPA). FA composition of the zooplankton fraction had comparatively poor correlations with taxonomic composition of the zooplankton. Thus, seasonal variations of FA composition of the zooplankton were determined primarily by seasonal changes in FA composition of the seston, rather than by taxonomic differences of FA profiles between rotifers, cyclopoids and cladocerans. FA composition of the seston was strongly affected by its taxonomic composition, namely by that of phytoplankton. According to CCA, the highest factor loadings pertained to diatoms interacting with their marker acids, including EPA, and cyanobacteria and greens, interacting with their marker acids. Ciliates and small rotifers composed considerable and sometimes major part of the seston biomass, but according to CCA their contributions to seasonal variations of the total FA profile of the seston were insignificant. This finding indirectly support the conclusion of the other authors, that the main source of FAs presented in ciliates and rotifers must be sought in algae and that they do not modify FA composition of food consumed, apart from repackaging it.Water temperature was the principal environmental parameter which drove the overall variations of FA composition. Factor loadings for the inorganic nutrients were comparatively negligible. The main contribution in the seasonal variation of FA composition of the seston was given by negative interaction between water temperature and percentage of EPA in the seston. В© 2009 Elsevier GmbH.
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6.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Ivanova E.A., Kravchuk E.S.
Заглавие : Seasonal distribution and fatty acid composition of littoral microalgae in the Yenisei River
Место публикации : Journal of Applied Phycology. - 2010. - Vol. 22, Is. 1. - С. 11-24. - ISSN 09218971 (ISSN) , DOI 10.1007/s10811-009-9418-9
Ключевые слова (''Своб.индексиров.''): fatty acids--riverine microalgae--taxa composition--temperature adaptations--algae--bacillariophyta--chlorophyta--cyanobacteria
Аннотация: We studied fatty acid (FA) composition of littoral microalgae in the fast-flowing oligotrophic river, the Yenisei, Siberia, monthly for 3 years. Seasonal dynamics of species composition had similar patterns in all the studied years. In springs, a pronounced dominance of filamentous green algae occurred, in summer and autumn diatoms were abundant, and in late autumn and winter epilithic biofilms consisted primarily of cyanobacteria and detritus. In general, FA composition of the algal periphytic community was dominated by 16:0, 16:1?7, 20:5?3, 14:0, and 18:3?3 throughout the studied period. Several groups of FAs, which had peculiar seasonal dynamics, were differentiated by statistical analysis based on a method of correlation graphs. The seasonal changes in FA composition could be partly explained by the seasonal succession of species composition of the community. Besides, we found that populations of both diatom and green algae grown in summer at a higher water temperature were lower in polyunsaturated fatty acids than those in spring, at a lower temperature. Hence, we suppose that the regular seasonal dynamics of FA composition of the studied littoral microalgae was driven both by changes in species composition and by temperature adaptations of the algal populations. The highest content of essential polyunsaturated FAs, eicosapentaenoic and docosahexaenoic acids, in the spring "psychrophilic" populations of diatoms could make them of the higher nutritive value for zoobenthic primary consumers. В© Springer Science+Business Media B.V. 2009.
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7.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Anishchenko O.V., Gladyshev M.I., Kravchuk E.S., Ivanova E.A., Gribovskaya I.V., Sushchik N.N.
Заглавие : Seasonal variations of metal concentrations in periphyton and taxonomic composition of the algal community at a Yenisei River littoral site
Место публикации : Central European Journal of Biology. - 2010. - Vol. 5, Is. 1. - С. 125-134. - ISSN 1895104X (ISSN) , DOI 10.2478/s11535-009-0060-y
Ключевые слова (''Своб.индексиров.''): heavy metals--periphyton--seasonal dynamics--algae--chlorophyta--cyanobacteria--ulothrix zonata
Аннотация: The concentrations of metals K, Na, Ca, Mg, Fe, Mn, Zn, Cu, Ni, Pb, Co and Cr, in the water and periphyton (epilithic algal communities) were studied at a site in the middle stream of the Yenisei River (Siberia, Russia) during three years using monthly sampling frequencies. Despite considerable seasonal variations in aquatic concentrations of some metals, there was no correlation between metal contents in the water and in periphyton. Seasonal concentration variations of some metals in periphyton were related to the species (taxonomic) composition of periphytic microalgae and cyanobacteria. Enhanced levels of Ni and Co in periphyton in late autumn, winter, and early spring were likely caused by the predominance of cyanobacteria in the periphytic community, and annual maximum levels of K in periphyton in late spring and early summer were attributed to the domination of Chlorophyta, primarily Ulothrix zonata. В© Versita Warsaw and Springer-Verlag Berlin Heidelberg.
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8.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakov V.I., Anishchenko O.V., Ivanova E.A., Gladyshev M.I., Sushchik N.N.
Заглавие : Estimation of periphytic microalgae gross primary production with DCMU-fluorescence method in Yenisei River (Siberia, Russia)
Место публикации : Journal of Applied Phycology. - 2008. - Vol. 20, Is. 3. - С. 289-297. - ISSN 09218971 (ISSN) , DOI 10.1007/s10811-007-9246-8
Ключевые слова (''Своб.индексиров.''): chlorophyll fluorescence--gross primary production--periphytic microalgae
Аннотация: Periphyton (epilithon) gross primary production (GPP) was estimated using the DCMU-fluorescence method in the Yenisei River. In the unshaded littoral zone, chlorophyll a concentration (Chl a) and GPP value varied from 0.83 to 973.74 mg m-2and 2-304,425 O2 m-2 day -1 (0.64-95 133 mg C m-2 day-1), respectively. Positive significant correlation (r=0.8) between daily GPP and periphyton Chl a was found. Average ratio GPP:Chl a for periphyton was 36.36 mg C mg Chl a m -2 day-1. The obtained GPP values for the Yenisei River have a high significant correlation with values predicted by a conventional empirical model for stream periphyton. We concluded that the DCMU-fluorescence method can be successfully used for measuring of gross primary production of stream phytoperiphyton at least as another useful tool for such studies. В© 2007 Springer Science+Business Media B.V.
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9.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Dubovskaya O.P., Makhutova O.N., Kalachova G.S.
Заглавие : Influence of sestonic elemental and essential fatty acid contents in a eutrophic reservoir in Siberia on population growth of Daphnia (longispina group)
Место публикации : Journal of Plankton Research. - 2006. - Vol. 28, Is. 10. - С. 907-917. - ISSN 01427873 (ISSN) , DOI 10.1093/plankt/fbl028
Ключевые слова (''Своб.индексиров.''): eutrophic environment--fatty acid--multiple regression--particulate organic nitrogen--plankton--population growth--reservoir--seston--eurasia--siberia--daphnia
Аннотация: Dependence of specific integral population growth rate or specific production rate of populations with random size and age structure on elemental and essential fatty acid composition of natural seston was studied in a laboratory flow-through system using a multiple regression. Under a comparatively high content of particulate phosphorus (P), the best single predictor of the growth appeared to be content of eicosapentaenoic acid (EPA, 20:5?3), as it was found earlier for somatic growth of even-aged populations of Daphnia. Regression equation with two independent variables, EPA and particulate nitrogen (N), gave a significantly better fit for the population growth as the dependent variable than the equation with EPA as the single independent variable. Interestingly, there was no significant correlation between N as the single variable and the growth. The essential ?3 fatty acid and N appeared to be complementary indicators of the food quality, which probably limited different constituents (reproductive and somatic) of the integral population growth under the high content of particulate P. В© The Author 2006. Published by Oxford University Press. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kravchuk E.S., Ivanova E.A., Gladyshev M.I.
Заглавие : Seasonal dynamics of akinetes of Anabaena flos-aquae in bottom sediments and water column of small Siberian reservoir
Место публикации : Aquatic Ecology. - 2006. - Vol. 40, Is. 3. - С. 325-336. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-006-9031-9
Ключевые слова (''Своб.индексиров.''): akinetes--cyanobacteria--cyanobacterial blooms--resting stages--abundance--alga--intertidal environment--open water--population dynamics--reservoir--sediment--water column--eurasia--siberia--anabaena--anabaena flos-aquae--cyanobacteria
Аннотация: Seasonal dynamics of Anabaena flos-aquae (Lyngb.) Breb., including vegetative cells, akinetes and akinete envelopes, in bottom sediments and water column at both littoral and deeper central stations of a small Siberian reservoir was studied. Two types of akinetes were observed: in the first half of summer Anabaena formed akinetes, which served for vegetative reproduction and germinated in water column soon after differentiation, while in the second half of summer the akinetes produced served as a resting stages, which were deposited to bottom sediments. Canonical correlation analyses revealed that decrease of water temperature was the main environmental factor that stimulated the akinete formation. In contrast to the general opinion, concentration of inorganic phosphorus slightly, but positively influenced the akinete formation. Thus, akinetes formed in response to the temperature decrease, needs a certain level of this nutrient. At littoral and open-water stations abundance and seasonal dynamics of akinetes in water column and their sinking pattern were very similar. However, seasonal dynamics of abundance of akinetes in sediments in these two reservoir locations differed: whereas the abundance of akinetes in open water increased permanently during the summer, that in the littoral decreased soon after their sedimentation. The cause for decrease in abundance of akinetes in bottom sediments in winter is unknown. В© Springer 2006.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Makhutova O.N., Kalachova G.S., Kravchuk E.S., Ivanova E.A.
Заглавие : Associating particulate essential fatty acids of the ?3 family with phytoplankton species composition in a Siberian reservoir
Место публикации : Freshwater Biology. - 2004. - Vol. 49, Is. 9. - С. 1206-1219. - ISSN 00465070 (ISSN) , DOI 10.1111/j.1365-2427.2004.01263.x
Ключевые слова (''Своб.индексиров.''): cyanobacteria--diatoms--fatty acids--food quality--species composition--cyanobacterium--diatom--fatty acid--food quality--food web--growing season--phytoplankton--reservoir--eurasia--siberia--anabaena--anabaena flos-aquae--aphanizomenon--aphanizomenon flos-aquae--bacillariophyta--cyanobacteria--cyclotella--dinophyceae--flos--microcystis--microcystis aeruginosa--planktothrix--planktothrix agardhii--stephanodiscus
Аннотация: 1. We studied variation in the composition of fatty acids in the seston of a small freshwater reservoir with changes in phytoplankton composition during four growth seasons. We focused on the dynamics of the ?3 fatty acids because of their potential importance for zooplankton nutrition. 2. Total diatoms were related to the 20:5?3 fatty acid (eicosapentaenoic, EPA) content in seston. Among two dominant diatom genera, Cyclotella was not associated with EPA content. In contrast, there was a significant correlation between Stephanodiscus and the percentage contribution and content of EPA throughout the study. Hence, freshwater diatoms can differ strongly in content of the essential EPA. 3. We considered abundant cyanobacteria as a potential source of 18:3?3 fatty acid (linolenic, ALA) to aquatic food webs. Among four dominant cyanobacteria species, two (Anabaena flos-aquae and Planktothrix agardhii) showed significant correlation with the ALA content of the seston, while the other two (Aphanizomenon flos-aquae and Microcystis aeruginosa) did not. 4. Dinophyta had a relatively high level of 22:6?3 (docosahexaenoic, DHA) for freshwater species and can be also a source of EPA to aquatic food webs. 5. Our results show that various species of diatoms as well as cyanobacteria can be of contrasting nutritional value for zooplankton because of their different content of the essential PUFAs. Diatoms, which are low in EPA, could not be considered as a valuable food, while some field populations of cyanobacteria might be valuable sources of essential ALA.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Kalachova G.S., Kravchuk E.S., Dubovskaya O.P., Ivanova E.A.
Заглавие : Particulate fatty acids in two small Siberian reservoirs dominated by different groups of phytoplankton
Место публикации : Freshwater Biology. - 2003. - Vol. 48, Is. 3. - С. 394-403. - ISSN 00465070 (ISSN) , DOI 10.1046/j.1365-2427.2003.01009.x
Ключевые слова (''Своб.индексиров.''): cyanobacteria--daphnids--diatoms--fatty acids--alga--cyanobacterium--fatty acid--periodicity--phytoplankton--reservoir--seston--algae--bacillariophyta--chlorophyta--cyanobacteria--daphnia--dinophyceae--euglenida--eukaryota
Аннотация: 1. We studied the composition of fatty acids (FAs) in the seston from two small freshwater reservoirs (Bugach and Lesnoi) with distinct periodicity of domination by cyanobacteria and eukaryotic algae during the growth season. 2. The diatoms in the both reservoirs were characterised by a high content of 14:0 and C16 unsaturated acids, whereas that of the essential FA 20:5?3 [eicosapentanoic acid (EPA)] was low. The correlation between this polyunsaturated FA (PUFA) and diatom biomass was not significant in either reservoir. The percentage of 20:5?3 in seston significantly correlated with the biomass of euglenophyta in Bugach and dinophyta in Lesnoi. Hence the diatoms, usually referred as a valuable food for zooplankton, were not an important source of the essential PUFA in these systems. 3. The dominant cyanobacteria in Bugach, and the green algae in Lesnoi, both contained the same marker acids: 18:3?3 and 18:2?6. Hence, a discrimination between these two phytoplanktonic groups on the basis of FA biomarkers may be difficult in some cases. 4. We found no significant correlation between the content of 20:5?3 in seston and the biomass of the dominant daphniids in either reservoir. This is contrary to expectations, based on the literature, that EPA is generally important. Rather, the biomass of the two dominant Daphnia species in Bugach correlated strongly with the content of 18:3?3 in the seston. The cyanobacteria were a probable source of this ?3 FA for Daphnia. We conclude that EPA is not always important for Daphnia populations although, in such cases, some other PUFA (e.g. 18:3?3) might be related to their growth.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Gribovskaya I.V., Moskvicheva A.V., Muchkina E.Y., Chuprov S.M., Ivanova E.A.
Заглавие : Content of metals in compartments of ecosystem of a Siberian pond
Место публикации : Archives of Environmental Contamination and Toxicology. - 2001. - Vol. 41, Is. 2. - С. 157-162. - ISSN 00904341 (ISSN) , DOI 10.1007/s002440010233
Ключевые слова (''Своб.индексиров.''): aluminum--cadmium--calcium--chromium--copper--heavy metal--iron--lead--magnesium--manganese--nickel--potassium--sodium--zinc--aquatic ecosystem--biological uptake--heavy metal--pond--article--bioaccumulation--ecosystem--fish--nonhuman--pond--priority journal--russian federation--sediment--soil pollution--water contamination--animals--ecosystem--environmental monitoring--fishes--geologic sediments--invertebrates--metals, heavy--plants--water pollutants--russian federation
Аннотация: During three field seasons (June-September) of 1997-99 contents of Na, K, Ca, Mg, Fe, Mn, Zn, Cu, Al, Cr, Ni, Cd, and Pb were determined in compartments of ecosystem (surrounding soils, bottom sediments, water, zoobenthos, macrophytes, and fish) of a fish and recreation pond situated at the edge of Krasnoyarsk City (Siberia, Russia). Contents of most parts of metals in soils, water, and macrophytes significantly correlated with each other. As concluded, their contents were determined by natural, general, geochemical peculiarities of the region. Heavy metals, contents of which were higher than federal upper limits of concentration, were revealed. In muscles of fish with different feeding spectra - crucian and perch - concentrations of some metals differed significantly; correlation graphs for metals also had different structures. Comparison of our data with those on diverse aquatic ecosystems of Siberia, Europe, North America, and China published in the last decade was carried out. It was concluded that a distribution of heavy metals in the compartments of an aquatic ecosystem presently have to be determined for each particular water body until general regularities are discovered.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gros J.B., Lasseur Ch., Tikhomirov A.A., Manukovsky N.S., Kovalev V.S., Ushakova S.A., Zolotukhin I.G., Tirranen L.S., Karnachuk R.A., Dorofeev V.Yu.
Заглавие : Testing soil-like substrate for growing plants in bioregenerative life support systems
Место публикации : Advances in Space Research. - 2005. - Vol. 36, Is. 7. - С. 1312-1318. - ISSN 02731177 (ISSN) , DOI 10.1016/j.asr.2005.05.079
Ключевые слова (''Своб.индексиров.''): life support system--matter recycling--plants--soil-like substrate--biodiversity--correlation methods--growth kinetics--hormones--plants (botany)--recycling--soils--bioregeneration--life support system--matter recycling--soil-like substrate--space research
Аннотация: We studied soil-like substrate (SLS) as a potential candidate for plant cultivation in bioregenerative life support systems (BLSS). The SLS was obtained by successive conversion of wheat straw by oyster mushrooms and worms. Mature SLS contained 9.5% humic acids and 4.9% fulvic acids. First, it was shown that wheat, bean and cucumber yields as well as radish yields when cultivated on mature SLS were comparable to yields obtained on a neutral substrate (expanded clay aggregate) under hydroponics. Second, the possibility of increasing wheat and radish yields on the SLS was assessed at three levels of light intensity: 690, 920 and 1150 ?mol m-2 s-1 of photosynthetically active radiation (PAR). The highest wheat yield was obtained at 920 ?mol m-2 s-1, while radish yield increased steadily with increasing light intensity. Third, long-term SLS fertility was tested in a BLSS model with mineral and organic matter recycling. Eight cycles of wheat and 13 cycles of radish cultivation were carried out on the SLS in the experimental system. Correlation coefficients between SLS nitrogen content and total wheat biomass and grain yield were 0.92 and 0.97, respectively, and correlation coefficients between nitrogen content and total radish biomass and edible root yield were 0.88 and 0.87, respectively. Changes in hormone content (auxins, gibberellins, cytokinins and abscisic acid) in the SLS during matter recycling did not reduce plant productivity. Quantitative and species compositions of the SLS and irrigation water microflora were also investigated. Microbial community analysis of the SLS showed bacteria from Bacillus, Pseudomonas, Proteus, Nocardia, Mycobacterium, Arthrobacter and Enterobacter genera, and fungi from Trichoderma, Penicillium, Fusarium, Aspergillus, Mucor, Botrytis, and Cladosporium genera. В© 2005 COSPAR. Published by Elsevier Ltd. All rights reserved.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Kalachova G.S., Guseynova V.E.
Заглавие : Rapid assay of fatty acid composition using a portable high-performance liquid chromatograph for monitoring aquatic ecosystems
Место публикации : Journal of Chromatography A. - 1995. - Vol. 695, Is. 2. - С. 223-228. - ISSN 00219673 (ISSN) , DOI 10.1016/0021-9673(94)01090-2
Ключевые слова (''Своб.индексиров.''): fatty acid--alga--article--culture medium--ecology--high performance liquid chromatography--instrument--methodology--priority journal--ultraviolet spectrophotometry--water analysis
Аннотация: The chromatographic conditions presented allowed the separation of the nitrophenacyl derivatives of standards of eleven free fatty acids (FFA) using a portable high-performance chromatograph, suitable for use aboard a research vessel. A statistically significant linear correlation between UV absorbance and amount of the analytes injected was obtained. The method was tested on FFA from algae cultural media. The method can be used for the ecological monitoring of natural waters.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Berzhanzkaya L.Y., Gitel'zon I.I., Fish A.M.
Заглавие : Bioluminescence of bacteria: a rhythmic process
Место публикации : BIOPHYSICS. - 1973. - Vol. 18, Is. 2. - С. 293-301. - ISSN 00063509 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacterium--computer--in vitro study--methodology--microorganism--theoretical study
Аннотация: New results obtained in the detection of rhythms in the low frequency range in the light signal of a small number of photo bacteria are reported. The luminescence of a small number of bacteria was recorded with an apparatus the sensitivity of which was 12 quanta/pulse. To check the possible rhythms in the luminescence of the bacteria, correlation analysis was made of the interpulse intervals of the detection of the signal with a computer. This revealed a definite rhythm in the bacterial luminescence the fundamental frequency of which lies in the region 8 c/s. Profound modulation of a bioluminescent signal at this and multiple frequencies was demonstrated. The harmonic components found in the spectrum of the signal refute the existing view of the continuous character of bacterial luminescence.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tolomeev A.P., Dubovskaya O.P., Sushchik N.N., Makhutova O.N., Kalacheva G.S.
Заглавие : Differentiated influence of quality of seston (Content of C, N, P, and polyunsaturated fatty acids) on rate of somatic and generative growth of Daphnia
Место публикации : Contemporary Problems of Ecology. - 2012. - Vol. 5, Is. 4. - С. 450-458. - ISSN 19954255 (ISSN) , DOI 10.1134/S1995425512040130
Ключевые слова (''Своб.индексиров.''): c: n: p--daphnia--fatty acids--food quality--generative growth--somatic growth--carbon--crustacean--data set--fatty acid--food quality--growth rate--nitrogen--phosphorus--reservoir--seston--bugach reservoir--krasnoyarsk [russian federation]--russian federation--animalia--daphnia
Аннотация: The data set obtained in 19 experiments aimed at studying the growth of Daphnia of the longispina group on natural seston of the Bugach water reservoir (Krasnoyarsk) was used to analyze the relationship between the parameters of somatic and generative growth of the studied animals and the amount and quality of food. Depending on the quality of the seston, two models of the development of Daphnia were proposed. It was demonstrated that, despite the existing positive correlation between somatic and generative growth, a noticeable fraction of the variations of the specific rate of generative production is determined by external factors of the environment, such as the relative content of N and ?-linolenic acid (N: C and ALA: C) in seston. В© 2012 Pleiades Publishing, Ltd.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Isaev A.S., Soukhovolsky V.G., Khlebopros R.G.
Заглавие : Model approaches to description of critical phenomena in forest ecosystems
Место публикации : Contemporary Problems of Ecology. - 2011. - Vol. 4, Is. 7. - С. 699-705. - ISSN 19954255 (ISSN) , DOI 10.1134/S1995425511070010
Ключевые слова (''Своб.индексиров.''): critical phenomena--ecosystem--modeling--phase transitions--population--population dynamics--data set--ecosystem modeling--forest ecosystem--forest fire--insect--optimization--population dynamics--succession--hexapoda
Аннотация: Methodological bases of optimization modeling approach are considered in regard to describing critical phenomena in forest ecosystems. Models of insects' outbreaks, forest successions, forest fires as second order phase transitions are proposed. It is shown that there is fair correlation between model calculations and observations data. В© 2011 Pleiades Publishing, Ltd.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sid'ko A.F., Vasil'yev V.A.
Заглавие : Spectral brightness of inland waters and its correlation with phytoplankton content and hydrooptical characteristics
Место публикации : Hydrobiological Journal. - 1988. - Vol. 24, Is. 5. - С. 64-71. - ISSN 00188166 (ISSN)
Ключевые слова (''Своб.индексиров.''): cyanobacteria--diatom--light attenuation--pyrrhophyte--spectral brightness--ussr, dnieper--ussr, krasnoyarsk reservoir--algae--bacillariophyta--chlorophyta--cyanobacteria--dinophyceae--uncultured cyanobacterium
Аннотация: Spectral brightness coefficients are correlated with indices of vertical attenuation of horizontal irradiance, water transparency, phytoplankton chlorophyll content, and phytoplankton distribution. Data were obtained from Krasnoyarsk Reservoir and the Dnieper reservoirs. When diatoms and pyrrhophytes predominate, the maximum spectral brightness is in the vicinity of 580 nm, but when blue-green algae predominate the maxima are in the vicinity of 550 and 720 nm. -from Authors
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sid'ko A.F.
Заглавие : Remote assay for chlorophyll photosynthetic potential of crops on the example of wheat
Место публикации : Biol. Bull: MAIK NAUKA/INTERPERIODICA, 2004. - Vol. 31, Is. 5. - С. 450-456. - 7. - ISSN 1062-3590, DOI 10.1023/B:BIBU.0000043769.80086.02
Примечания : Cited References: 20
Аннотация: An optical remote assay for biological production of crops in the field during the vegetation period is proposed. Our calculations demonstrate a good correlation between the S(t) value and crop yield (in the range from 0.85 to 0.90); the higher is S(t) for the vegetation period, the higher is the crop yield. S(t) value can be used to calculate the increase in dry and wet biomass for various crops during the whole vegetation period with an error of less than 10-12%. The absolute error of crop yield for 110 cultivars of wheat, oats, and barley during the experimental period was +/-3-3.5 quintal/ha with the mean crop yield of 14-38 quintal/ha.
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