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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Adamovich V.V., Rogozin D.Yu., Degermendzhi A.G.
Заглавие : Developing the control criterion for a continuous culture of microorganisms
Место публикации : Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 5-16. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): chemostat--control criterion--control factor--microorganism population--sensitivity coefficients--algorithm--bacterium--biological model--biomass--culture medium--ecosystem--growth, development and aging--methodology--microbiological examination--review--algorithms--bacteria--bacteriological techniques--biomass--culture media--ecosystem--models, biological
Аннотация: A short survey and critical analysis of previously proposed criteria for growth control of populations of microorganisms in the chemostat are presented. Based on the analysis of a mathematical model of the steady-state of a microbial population in the chemostat, an adequate control criterion is suggested, along with a method to identify the corresponding regulating factors. The new control criterion is expressed as a product of the factor transformation coefficient and the biomass sensitivity coefficient (SC) with respect to the change of the factor at the chemostat inlet (referred to in the sequel as the biomass SC). The control criterion determines the strength of the control exerted by this or that factor. The method of determination of the regulating factors consists in experimental determination of the real SCs for factors and the biomass and in calculating on this basis the corresponding ideal SCs for constant factor transformation coefficients. The ideal SCs are shown to add up to an integer value, a constraint that we call "quantization" relationships. Such relationships are used to test the completeness of the drawn list of control factors. The proposed method was applied to our own and literature data.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Adamovich V.V., Rogozin D.Yu., Degermendzhi A.G.
Заглавие : Developing the control criterion for a continuous culture of microorganisms
Место публикации : Microbiology. - 2005. - Vol. 74, Is. 1. - С. 1-11. - ISSN 00262617 (ISSN) , DOI 10.1007/s11021-005-0021-z
Ключевые слова (''Своб.индексиров.''): chemostat--control criterion--control factor--microorganism population--sensitivity coefficients
Аннотация: A short survey and a critical analysis of previously proposed criteria for growth control of populations of microorganisms in the chemostat are presented. Based on the analysis of a mathematical model of the steady state of a microbial population in the chemostat, an adequate control criterion is suggested, along with a method to identify the corresponding control factors. The new control criterion is expressed as a product of the factor transformation coefficient and the sensitivity coefficient (SC) of the biomass with respect to the change in the factor at the chemostat inlet (hereinafter, the biomass SC). The control criterion determines the strength of the control exerted by a factor. The method of determining control factors consists in experimental determination of the real SCs for factors and the biomass and calculation on this basis of the corresponding ideal SCs, assuming constant factor transformation coefficients. The ideal SCs are shown to add up to integer values, a constraint that we call quantization relationships. Such relationships are used to test the completeness of the list of control factors. The proposed method was applied to both our own and literature data. В© 2005 Pleiades Publishing, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kiselev E.G., Shishatskaya E.I., Zhila N.O., Boyandin A.N., Syrvacheva D.A., Vinogradova O.N., Kalacheva G.S., Vasiliev A.D., Peterson I.V.
Заглавие : Cell growth and accumulation of polyhydroxyalkanoates from CO2 and H2 of a hydrogen-oxidizing bacterium, Cupriavidus eutrophus B-10646
Место публикации : Bioresource Technology. - 2013. - Vol. 146. - С. 215-222. - ISSN 09608524 (ISSN) , DOI 10.1016/j.biortech.2013.07.070
Ключевые слова (''Своб.индексиров.''): autotrophic synthesis--cupriavidus eutrophus--polyhydroxyalkanoates
Аннотация: Synthesis of polyhydroxyalkanoates (PHAs) by a new strain of Cupriavidus - Cupriavidus eutrophus B-10646 - was investigated under autotrophic growth conditions. Under chemostat, at the specific flow rate D=0.1h-1, on sole carbon substrate (CO2), with nitrogen, sulfur, phosphorus, potassium, and manganese used as growth limiting elements, the highest poly(3-hydroxybutyrate) [P(3HB)] yields were obtained under nitrogen deficiency. In batch autotrophic culture, in the fermenter with oxygen mass transfer coefficient 0.460h-1, P(3HB) yields reached 85% of dry cell weight (DCW) and DCW reached 50g/l. Concentrations of supplementary PHA precursor substrates (valerate, hexanoate, ?-butyrolactone) and culture conditions were varied to produce, for the first time under autotrophic growth conditions, PHA ter- and tetra-polymers with widely varying major fractions of 3-hydroxybutyrate, 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate monomer units. Investigation of the high-purity PHA specimens showed significant differences in their physicochemical and physicomechanical properties. В© 2013 Elsevier Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ganusov V.V., Bril'kov A.V., Pechurkin N.S.
Заглавие : Mathematical modeling of population dynamics of unstable plasmid-containing bacteria during continuous cultivation in a chemostat
Место публикации : Biofizika. - 2000. - Vol. 45, Is. 5. - С. 908-914. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--bioreactor--escherichia coli--fermentation--genetics--growth, development and aging--photobacterium--plasmid--theoretical model--bioreactors--escherichia coli--fermentation--models, theoretical--photobacterium--plasmids
Аннотация: A structural approach to studying the regularities of the population dynamics of unstable recombinant bacterial strains in a chemostat was elaborated. The approach is based on the mathematical modeling of cell distribution in a population with different numbers of plasmid copies. The effect of decreased selective preference of plasmidless variants of the recombinant strain in the chemostat, which is related to a decrease in the number of plasmid copies in cells upon long-term incubation was analyzed. It is shown that the time of half-elimination of plasmids from the bacterial population in the steady state in the chemostat T1/2 does not depend on the maximum number of plasmid copies in cells N but is determined only by the mean time of generation g and the probability of the loss of one plasmid copy tau. The dependence of the preference of bacterial plasmidless variants on the efficiency of expression of genes cloned into plasmids in chemostat was analyzed using the recombinant strain E. coli Z905, whose plasmids pPHL-7 contain cloned genes for the luminescence system of marine luminescing bacteria Photobacterium leiognathi.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Ladygina V.P., Pechurkin N.S., Terskov I.A.
Заглавие : Influence of the abiogenic link on a predator population in the development of a simple biological community in a chemostat
Место публикации : Doklady Biochemistry. - 1976. - Vol. 227, Is. 1-6. - С. 173-175. - ISSN 00124958 (ISSN)
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A.
Заглавие : A new enzymatic technique to estimate the efficiency of microbial degradation of pollutants
Место публикации : Advances in Space Research. - 1997. - Vol. 20, Is. 10. - С. 2049-2052. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): catalase--hydrogen peroxide--aerobic metabolism--article--bacterium--biomass--bioremediation--enzymology--metabolism--methodology--microbiology--sewage--waste management--water management--aerobiosis--bacteria--biodegradation, environmental--biomass--catalase--hydrogen peroxide--sewage--waste management--water microbiology--water purification
Аннотация: Dynamics of active sludge microorganism activity in aerotanks under chemostat conditions has been studied. Dependence of microorganism catalase activity has been found to depend on residual substrate concentration in proportion to the biomass of microorganisms. Experimental data and field observations has formed the basis to develop a technique to evaluate in relative units the amount of the substrate consumed by biocenosis of the active sludge in the air tanks of purification facilities. В© 1997 COSPAR. Published by Elsevier Science Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : POPOVA L.Y., LUTSKAYA N.I., BOGUCHAROV A.A., BRILKOV A.V., PECHURKIN N.S.
Заглавие : POPULATION-STRUCTURE DYNAMICS OF A RECOMBINANT STRAIN OF ESCHERICHIA-COLI IN CONTINUOUS CULTURE
Колич.характеристики :6 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1992. - Vol. 61, Is. 4. - С. 417-422. - ISSN 0026-2617
Примечания : Cited References: 10
Аннотация: We studied the population structure of Escherichia coli strain Z905, carrying the recombinant plasmid of the Ap(r)Lux+ phenotype, in continuous culture in a chemostat. We found that the maintenance of a stable, defined ratio of plasmidless to plasmid-carrying cells in the population depended primarily on the presence in the medium of the selective factor (ampicillin), and also on the nature of the growth-limiting carbon and energy source.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr' A.P., Mogil'Naya O.A., Gurevich Y.L., Babkina E.A.
Заглавие : Colony structure of a consortium of nitrifying bacteria
Место публикации : Mikrobiologiya. - 2001. - Vol. 70, Is. 1. - P98-105. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): colony architectonics--electron microscopy--nitrifying bacteria--article--bacterium--bioreactor--culture medium--electron microscopy--growth, development and aging--nitrogen fixation--ultrastructure--bacteria--bioreactors--culture media--microscopy, electron--nitrogen fixation
Аннотация: Colonies produced by a consortium of nitrifying bacteria were studied using light and electron microscopy. The colonies were obtained by direct plating of inoculum from a two-stage nonsterile chemostat fermentor and by repeatedly passing the microbial community of the fermenter through selective media containing ammonium or nitrite. The colonies studied can be characterized by a specific combination of six types of cells differing in their ultrastructure and spatial location within the colony. The types of cells occurring within a given colony were found to depend on the nitrogen compound present in the medium. As a result of our study, morphological features of colonial bacterial communities were preliminarily identified. The proposed approach can be viewed as a method to describe microbial associations and communities.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : MEZHEVIKINA I.V., PECHURKIN N.S., TERSKOV I.A.
Заглавие : INFLUENCE OF THE ENERGY MAINTENANCE OF ORGANISMS ON THE DYNAMICS OF THE PREDATOR-PREY SYSTEM IN A CHEMOSTAT
Колич.характеристики :3 с
Место публикации : DOKLADY AKADEMII NAUK SSSR: MEZHDUNARODNAYA KNIGA, 1981. - Vol. 257, Is. 6. - P1500-1502. - ISSN 0002-3264
Примечания : Cited References: 9
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10.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Sarangova A.B., Somova L.A.
Заглавие : A new enzymatic technique to estimate the efficiency of microbial degradation of pollutants
Колич.характеристики :4 с
Место публикации : LIFE SCIENCES: LIFE SUPPORT SYSTEMS STUDIES-I. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1997. - Vol. 20: F4.6, F4.8, F4.2 and F4.9 Symposia of COSPAR Scientific Commission F on Life Sciences - Life Support System Studies-I, at the 31st COSPAR Scientific Assembly (JUL 14-SEP 21, 1996, BIRMINGHAM, ENGLAND), Is. 10. - P2049-2052. - ISBN 0273-1177, DOI 10.1016/S0273-1177(97)00940-X. - ISBN 0-08-043307-3
Примечания : Cited References: 4
Аннотация: Dynamics of active sludge microorganism activity in aerotanks under chemostat conditions has been studied. Dependence of microorganism catalase activity has been found to depend on residual substrate concentration in proportion to the biomass of microorganisms. Experimental data and field observations has formed the basis to develop a technique to evaluate in relative units the amount of the substrate consumed by biocenosis of the active sludge in the air tanks of purification facilities. (C) 1997 COSPAR. Published by Elsevier Science Ltd.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr' A.P., Mogil'naya O.A., Gurevich Y.L., Babkina E.A.
Заглавие : Colony structure of a consortium of nitrifying bacteria
Колич.характеристики :7 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 70, Is. 1. - P84-90. - ISSN 0026-2617, DOI 10.1023/A:1004853123326
Примечания : Cited References: 6
Предметные рубрики: COMMUNITY
Ключевые слова (''Своб.индексиров.''): electron microscopy--colony architectonics--nitrifying bacteria
Аннотация: Colonies produced by a consortium of nitrifying bacteria were studied using fight and electron microscopy. The colonies were obtained by direct plating of inoculum from a two-stage nonsterile chemostat fermenter and by repeatedly passing the microbial community of the fermenter through selective media containing ammonium or nitrite. The colonies studied can be characterized by a specific combination of six types of cells differing in their ultrastructure and spatial location within the colony. The types of cells occurring within a given colony were found to depend on the nitrogen compound present in the medium. As a result of our study, morphological features of colonial bacterial communities were identified. The proposed approach can be viewed as a method to describe microbial associations and communities.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pisman T.I., Bogdanova O.N.
Заглавие : A mathematical model of the interactions in the mixed culture of invertebrates and algae in the "producer-consumer" aquatic biotic cycle
Колич.характеристики :9 с
Место публикации : Aquat. Ecol.: KLUWER ACADEMIC PUBL, 2004. - Vol. 38, Is. 3. - P415-423. - ISSN 1386-2588
Примечания : Cited References: 19
Предметные рубрики: RESOURCE COMPETITION
Ключевые слова (''Своб.индексиров.''): competition--metabolite interaction--principle of competitive exclusion
Аннотация: This paper presents a mathematical model of interactions between two herbivorous invertebrates (ciliate Paramecium caudatum and rotifer Brachionus plicatilis) and two planktonic algae (Chlorella vulgaris and Scenedesmus quadricauda) spatially segregated in two compartments of a chemostat - type experimental microcosm system. The model mimics a "producer-consumer" aquatic biotic cycle, describing the dynamics of the mixed culture of ciliates and rotifers, as "consumer" compartment, feeding on the mixed algal culture, as "producer" compartment, under N-limiting conditions. We experimentally found that metabolites of the alga Scenedesmus produce an adverse effect on the reproduction of ciliate Paramecium. Taking this effect into account improved the behavior of the model, the results of which came into qualitative agreement with the experimental results. Both our experimental and modeling approaches demonstrated that, even in conditions of a spatially - segregated "producer-consumer" biotic cycle, species coexistence is impossible either in the mixed algal culture or in the mixed invertebrate culture. Scenedesmus excluded Chlorella, whereas Brachionus excluded Paramecium.
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