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^a314.23.27.07.11^2VINITI
П 53

Получение, свойства и применение кальцийчувствительного фотопротеина из гидроида Obelia longissima [Текст] : научное издание / В. С. Бондарь [и др.] // Цитология. - 1991. - Т. 33, N 6. - С. 50-59 . - ISSN 0041-3771

ГРНТИ

31.23.27

РУБ 314.23.27.07.11
Рубрики:
ФОСФОПРОТЕИНЫ
   КАЛЬЦИЙ ЧУВСТВИТЕЛЬНЫЙ
   ВЫДЕЛЕНИЕ
   ГИДРОЛИЗ
   OBELIA LONGISSIMA
   OBELIN
   PHOSPHOMOTEIN
   CHROMATOGRAPHY
Аннотация: С помощью Ca{2}{+} - активируемого протеина белина измеряли конц-ию свободного ионизированного кальция в макрофагах (МФ) в покое и при действии различных стимуляторов. Использовали обелин, выделенный из гидроида Obelia longissima и очищенный с использованием гельфильтрации на Сефадексе, ИОХ на полисиле, гидрофобной хр-фии на фенил-сефарозе, повторной гель-фильтрации на Сефадексе. Нагрузку МФ фотопротеином проводили модифицированным методом "осмотического лизиса пиносом". Фоновое значение конц-ии цитоплазматич. кальция [Ca{2}{+}][j] в покоящихся МФ, зарегистрированное с помощью обелина, соответствует 0,5-0,7 мкМ. Сыворотка крупного рогатого скота (20%) стимулирует периодич. импульсное повышение [Ca{2}{+}][j] до 1,3 мкМ с быстрым падением практически до уровня фона, наблюдаемое в течение не менее 30 мин, NaF (20 мМ), добавленный к суспензии МФ, вызывает быстрое однократное увеличение [Ca{2}{+}][j] до 1,4 мкМ. АТФ (30-60 мкМ) стимулирует повышение [Ca{2}{+}][j] в МФ до 1,5 мкМ. цАМФ (20 мкМ) повышает конц-ию свободного внутриклеточного кальция до 1,5 мкМ с быстрым падением и возвращением за 30 с к уровню фона, при этом ЭГТА (2 мМ) не снимает стимулирующего эффекта цАМФ на МФ. При изменении внутриклеточного рН МФ в щелочную сторону эффект цАМФ качественно сохраняется, однако повышение [Ca{2}{+}][j] менее выражено. Закисление цитоплазмы вызывает качественное и количеств. изменение кальциевого ответа МФ на добавку цАМФ: [Ca{2}{+}][j] не повышается более чем до 1,3 мкМ, время достижения макс. уровня [Ca{2}{+}][j] и возвращения к исходному фону увеличено. Рассматриваются возможные мех-мы действия различных стимуляторов на МФ, сопровождающиеся повышением уровня внутриклеточного свободного кальция. Библ. 38. Россия, Ин-т биофизики СОРАН, Красноярск.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Бондарь, В.С.; Высоцкий, Е.С.; Гамалей, И.А.; Каулин, А.Б.

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Use of proZZ-obelin fusion protein in bioluminescent immunoassay [Text] / L. A. Frank, V. A. Illarionova, E. S. Vysotski // Biochem. Biophys. Res. Commun. - 1996. - Vol. 219, Is. 2. - P475-479, DOI 10.1006/bbrc.1996.0258. - Cited References: 21 . - 5. - ISSN 0006-291X

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
ESCHERICHIA-COLI
   EXPRESSION
   AEQUORIN
   PURIFICATION
   SYSTEM
Аннотация: Obelin is a photoprotein that emits light by Ca2+-binding. To develop a bioluminescent immunoassay based on the light emission property of obelin, we have expressed the apoobelin fusion protein with ZZ-domain of S. aureus protein A in E. coil by recombinant DNA techniques. The pro2Z-obelin expressed was purified by one-step affinity chromatography on IgG-Agarose. The purified proZZ-obelin has both the luminescent activity of obelin and the IgG-binding ability of ZZ-domain. The specific activity of fusion protein was 8.5 x 10(15) photons per mg of protein. (C) 1996 Academic Press, Inc.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Frank, L.A.; Illarionova, V.A.; Vysotski, E.S.

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The yellow bioluminescence bacterium, Vibrio fischeri Y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence FMN protein / V. N. Petushkov, B. G. Gibson, J. Lee // Biochemical and Biophysical Research Communications. - 1995. - Vol. 211, Is. 3. - P774-779, DOI 10.1006/bbrc.1995.1880 . - ISSN 0006-291X
Кл.слова (ненормированные):
riboflavin -- article -- bioluminescence -- fluorescence -- nonhuman -- priority journal -- protein analysis -- protein synthesis -- vibrio -- vibrionaceae -- Bacterial Proteins -- Chromatography, Gel -- Chromatography, Thin Layer -- Flavin Mononucleotide -- Flavoproteins -- Luminescence -- Riboflavin -- Spectrometry, Fluorescence -- Support, U.S. Gov't, P.H.S. -- Vibrio -- Bacteria (microorganisms) -- Photobacterium -- Vibrio -- Vibrio fischeri
Аннотация: The yellow bioluminescence Y1 strain of Vibrio fischeri can produce a 22 kDa protein with either FMN or riboflavin as a bound fluorophore. Both forms are active for shifting the bioluminescence spectral maximum. The fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. The bioluminescence activity of the riboflavin Y1 protein contrasts with the inactivity of the related Photobacterium type.

Scopus
Держатели документа:
Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, United States
Institute of Biophysics, Academy of Sciences of Russia (Siberian Branch), 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Gibson, B.G.; Lee, J.

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The value of fatty acid composition of tricylglycerols and polar lipids of seston at analysis of food spectra of microzooplancton in small reservoir Buguch / O. N. Makhutova, N. N. Sushchik, G. S. Kalacheva // Doklady Akademii Nauk. - 2004. - Vol. 395, Is. 4. - С. 562-565 . - ISSN 0869-5652
Кл.слова (ненормированные):
Algae -- Bacteria -- Chromatographic analysis -- Chromatography -- Organic compounds -- Food spectra -- Microzooplancton -- Polar lipids -- Microorganisms
Аннотация: Food spectrum of microzooplancton in fresh-water ecological systems is lowly studied area. However, these organisms play very important role in transfer of substance and energy in water reservoirs. Traditional method of analysis of food in the microzooplancton does not makes it possible to determine real assimilation of organic substance. Therefore, biochemical methods have been developed which are based on analysis of compositions of fatty acids of organic substance. This leads to determining of the food assimilation. Fatty-acid compositions of lipids allow specialists to determine specific groups of organisms such as bacteria, microalgae etc. Data of the analysis are presented.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Makhutova, O.N.; Sushchik, N.N.; Kalacheva, G.S.

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Study of the luminescence system of the soil enchytraeid Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae) / V. N. Petushkov, N. S. Rodionova, V. S. Bondar' // Doklady Biochemistry and Biophysics. - 2003. - Vol. 391, Is. 1-6. - P204-207, DOI 10.1023/A:1025105323648 . - ISSN 1607-6729
Кл.слова (ненормированные):
adsorption chromatography -- animal cell -- annelid worm -- article -- luminescence -- nonhuman -- purification -- animal -- drug antagonism -- isolation and purification -- metabolism -- physiology -- soil -- Animalia -- Annelida -- Clitellata -- Enchytraeidae -- edetic acid -- luciferase -- magnesium -- Animals -- Edetic Acid -- Luciferases -- Luminescent Measurements -- Magnesium -- Oligochaeta -- Soil

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Rodionova, N.S.; Bondar', V.S.

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Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system / D. Chang, Y. Liu, Y. Chen [et al.] // BMC Vet. Res. - 2020. - Vol. 16, Is. 1. - Ст. 202, DOI 10.1186/s12917-020-02422-3 . - ISSN 1746-6148
Кл.слова (ненормированные):
Baculovirus expression system -- Canine parvovirus -- VP2 protein -- canine parvovirus vaccine -- protein VP2 -- recombinant protein -- unclassified drug -- virus antibody -- virus vaccine -- affinity chromatography -- animal experiment -- antibody titer -- Article -- baculovirus expression system -- Canine parvovirus -- controlled study -- DNA transposition -- enzyme linked immunosorbent assay -- female -- fluorescence microscopy -- gene expression level -- hemagglutination inhibition -- hemagglutination inhibition test -- immunogenicity -- mouse -- nonhuman -- parvovirus infection -- protein expression -- Sf9 cell line -- vaccination -- Western blotting
Аннотация: Background: Canine parvovirus (CPV) is now recognized as a serious threat to the dog breeding industry worldwide. Currently used CPV vaccines all have their specific drawbacks, prompting a search for alternative safe and effective vaccination strategies such as subunit vaccine. VP2 protein is the major antigen targeted for developing CPV subunit vaccine, however, its production in baculovirus expression system remains challenging due to the insufficient yield. Therefore, our study aims to increase the VP2 protein production by using an improved baculovirus expression system and to evaluate the immunogenicity of the purified VP2 protein in mice. Results: The results showed that high-level expression of the full length VP2 protein was achieved using our modified baculovirus expression system. The recombinant virus carrying two copies of VP2 gene showed the highest expression level, with a productivity of 186 mg/L, which is about 1.4-1.6 fold that of the recombinant viruses carrying only one copy. The purified protein reacted with Mouse anti-His tag monoclonal antibody and Rabbit anti-VP2 polyclonal antibody. BALB/c mice were intramuscularly immunized with purified VP2 protein twice at 2 week intervals. After vaccination, VP2 protein could induce the mice produce high level of hemagglutination inhibition antibodies. Conclusions: Full length CPV VP2 protein was expressed at high level and purified efficiently. Moreover, it stimulated mice to produce high level of antibodies with hemmaglutination inhibition properties. The VP2 protein expressed in this study could be used as a putative economic and efficient subunit vaccine against CPV infection. © 2020 The Author(s).

Scopus
Держатели документа:
Henan Provincal Engineering and Technology Center of Health Products for Livestock and Poultry, Key Laboratory of Ecological Security, Collab. Innov. Ctr. of Water Secty. for Water Src. Reg. of Mid-line of S.-to-N. Diversion Proj. of Henan Prov., School of Agricultural Engineering, Nanyang Normal University, Nanyang, 473061, China
Institute of Biophysics, Siberian Branch, Russian Academy of Science, Federal Research Center Krasnoyarsk Science Center SB RAS, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Chang, D.; Liu, Y.; Chen, Y.; Hu, X.; Burov, A.; Puzyr, A.; Bondar, V.; Yao, L.

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Simultaneous Bioluminescent Immunoassay of Serum Total and IgG-Bound Prolactins / A. N. Kudryavtsev [et al.] // Anal. Chem. - 2012. - Vol. 84, Is. 7. - P3119-3124, DOI 10.1021/ac300444w. - Cited References: 10. - This work was supported in part by Grant No. 76 of the Russian Academy of Sciences, Siberian Branch and by the Program of the Government of Russian Federation "Measures to attract leading scientists to Russian educational institutions" (Grant No 11. G34.31.058). . - ISSN 0003-2700

РУБ Chemistry, Analytical
Рубрики:
PHOTOPROTEIN OBELIN
   POLYETHYLENE-GLYCOL
   MACROPROLACTINEMIA
   PRECIPITATION
   VALIDATION
Аннотация: Novel dual-analyte single-well bioluminescence immunoassay (BLIA) for total and IgG-bound prolactins was developed on the base of Ca2+-regulated photoprotein obelin mutants with altered color and kinetics of bioluminescence signal as reporters. The mutants W92F-H22E and Y138F were chemically conjugated with monoclonal mouse anti-hPRL and anti-hIgG immunoglobulins and thus displayed signals from total prolactin and IgG-bounded prolactin (macroprolactin) correspondingly. Bioluminescence of the reporters was simultaneously triggered by a single injection of Ca2+ solution and discriminated via bioluminescent signal spectral and time resolution. The developed microplate-based immunoassay allows detection of two prolactin forms in crude serum without additional manipulations (e.g., gel chromatography or PEG-precipitation). Total prolactin bioluminescence immunoassay in standard, control, and clinical sera offers high sensitivity and reproducibility. The BLIA results show good correlation with those obtained by RIA and immunoassay after gel chromatography.

Держатели документа:
[Kudryavtsev, Alexander N.
Krasitskaya, Vasilisa V.
Frank, Ludmila A.] Russian Acad Sci, Inst Biophys, Photobiol Lab, Siberian Branch, Krasnoyarsk 660036, Russia
[Kudryavtsev, Alexander N.
Krasitskaya, Vasilisa V.
Frank, Ludmila A.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Petunin, Alexei I.] DIAS Ltd, Krasnoyarsk 660036, Russia
[Burakov, Andrey Y.] Krasnoyarsk Reg Hosp 1, Krasnoyarsk 660022, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kudryavtsev, A.N.; Krasitskaya, V.V.; Petunin, A.I.; Burakov, A.Y.; Frank, L.A.

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Seasonal dynamics of fatty acids in the lipids of water moss Fontinalis antipyretica from the Yenisei River / G. S. Kalacheva [et al.] // Russian Journal of Plant Physiology. - 2009. - Vol. 56, Is. 6. - P795-807, DOI 10.1134/S1021443709060090 . - ISSN 1021-4437
Кл.слова (ненормированные):
Acetylenic acids -- Fatty acids -- Fontinalis antipyretica -- Seasonal dynamics -- Bryophyta -- Fontinalis antipyretica
Аннотация: Identification of lipid fatty acids (FA) and studying of their seasonal dynamics in water moss Fontinalis antipyretica from the Yenisei River were carried out by means of gas chromatography-mass spectrometry. FA composition of water moss was notable for a relatively low level of saturated acids and predominance of polyunsaturated acids (PUFA) with double bonds (accounting for more than 30% of total FA) and polyunsaturated acids with double and triple bonds (acetylenic acids, accounting for more than 40% of total FA). Among PUFA, ?- and ?-linolenic (18:3?3 and 18:3?6), arachidonic (20:4?6), and eicosapentaenoic (20:5?3) acids prevailed. Relative content of PUFA from ?3-family was the greatest in spring, and the level of PUFA from ?6-group was essentially the same throughout all the seasons. In the biomass of water moss, we identified seven acetylenic acids; among them octadeca-9,12-dien-6-ynoic (6a,9,12-18:3), octadeca-9,12,15-trien-6-ynoic (6a,9,12,15-18:4), and eicosa-11,14-dien-8-ynoic (8a,11,14-20:3) acids were predominant. For the first time, in the lipids of water moss we identified an acetylenic eicosa-11,14,17-trien-8-ynoic acid (8a,11,14,15-20:4). Relative content of acetylenic acids in the total FA was great throughout the entire period of investigation with the peak accumulation in summer. Owing to a steadily high level in the biomass of water moss and the lack of other producers of these acids in the ecosystem, acetylenic FA are highly specific biochemical markers useful for the investigation of trophic interactions between higher aquatic plants and zoobenthos. В© Pleiades Publishing, Ltd., 2009.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kalacheva, G.S.; Sushchik, N.N.; Gladyshev, M.I.; Makhutova, O.N.

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Rapid assay of fatty acid composition using a portable high-performance liquid chromatograph for monitoring aquatic ecosystems / N. N. Sushchik [et al.] // Journal of Chromatography A. - 1995. - Vol. 695, Is. 2. - P223-228, DOI 10.1016/0021-9673(94)01090-2 . - ISSN 0021-9673
Кл.слова (ненормированные):
fatty acid -- alga -- article -- culture medium -- ecology -- high performance liquid chromatography -- instrument -- methodology -- priority journal -- ultraviolet spectrophotometry -- water analysis
Аннотация: The chromatographic conditions presented allowed the separation of the nitrophenacyl derivatives of standards of eleven free fatty acids (FFA) using a portable high-performance chromatograph, suitable for use aboard a research vessel. A statistically significant linear correlation between UV absorbance and amount of the analytes injected was obtained. The method was tested on FFA from algae cultural media. The method can be used for the ecological monitoring of natural waters.

Scopus
Держатели документа:
Institute Biophysics, Akademgorodok, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sushchik, N.N.; Gladyshev, M.I.; Kalachova, G.S.; Guseynova, V.E.

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10.

Purification and partial spectral characterization of a novel luciferin from the luminous enchytraeid Fridericia heliota / V. N. Petushkov, N. S. Rodionova // Journal of Photochemistry and Photobiology B: Biology. - 2007. - Vol. 87, Is. 2. - P130-136, DOI 10.1016/j.jphotobiol.2007.03.006 . - ISSN 1011-1344
Кл.слова (ненормированные):
ATP -- Bioluminescence -- Earthworms -- Enchytraeid -- Luciferase -- Luciferin -- adenosine triphosphate -- luciferase -- luciferin -- animal cell -- anion exchange chromatography -- annelid worm -- article -- controlled study -- earthworm -- firefly -- luminance -- luminescence -- nonhuman -- priority journal -- protein analysis -- protein purification -- radiation absorption -- reversed phase liquid chromatography -- ultraviolet radiation -- Adenosine Triphosphate -- Animals -- Luciferases -- Luminescent Agents -- Oligochaeta -- Spectrum Analysis -- Enchytraeidae -- Fridericia heliota
Аннотация: A homogeneous luciferin preparation has been obtained from the luminous soil enchytraeid Fridericia heliota, which has an ATP-dependent luminescent system. A procedure for luciferin purification without losing fractions of active luciferase has been developed. The luciferin specific activity is 4000 times increased; its UV absorption spectrum maximum is 294 nm with a local minimum at 262 nm. The luciferin of the enchytraeid F. heliota is significantly different from firefly luciferin, whose luminescent reaction also requires ATP, and it also appears to have no similarities to other known luciferins. В© 2007 Elsevier B.V. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Petushkov, V.N.; Rodionova, N.S.

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11.

Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood / V. I. Sevastianov [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - P1029-1042, DOI 10.1163/156856203769231547 . - ISSN 0920-5063
Кл.слова (ненормированные):
?-hydroxy acids -- Endotoxins -- Hemocompatibility -- Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (PHB) -- bacterium lipopolysaccharide -- carbon -- complement -- copolymer -- hydroxyacid -- long chain fatty acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- valeric acid derivative -- adult -- article -- biofilm -- biotechnology -- blood analysis -- blood clotting -- blood compatibility -- cell function -- chemical analysis -- chemical composition -- complement activation -- concentration (parameters) -- controlled study -- gas chromatography -- hemostasis -- human -- human cell -- mass spectrometry -- micromorphology -- nonhuman -- priority journal -- purification -- quantitative analysis -- sampling -- synthesis -- thrombocyte adhesion -- Wautersia eutropha -- Biocompatible Materials -- Blood -- Blood Coagulation Tests -- Chromatography, Gas -- Complement Activation -- Cupriavidus necator -- Fatty Acids -- Humans -- Platelet Adhesiveness -- Polyesters -- Surface Properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

Scopus
Держатели документа:
Inst. of Transplantol. Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Inst. of Biophys. of Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sevastianov, V.I.; Perova, N.V.; Shishatskaya, E.I.; Kalacheva, G.S.; Volova, T.G.

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12.

Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood [Text] / V. I. Sevastianov [et al.] // J. Biomater. Sci.-Polym. Ed. - 2003. - Vol. 14, Is. 10. - P. 1029-1042, DOI 10.1163/156856203769231547. - Cited References: 34 . - ISSN 0920-5063

РУБ Engineering, Biomedical + Materials Science, Biomaterials + Polymer Science
Рубрики:
BIODEGRADABLE POLYESTERS
POLYMERS
Кл.слова (ненормированные):
polyhydroxyalkanoates (PHAs) -- polyhydroxybutyrate (PHB) -- poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) -- endotoxins -- beta-hydroxy acids -- hemocompatibility
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C-16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only beta-OH-C-14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Russian Minist Hlth, Inst Transplantol Artificial Organs, Moscow 123182, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sevastianov, V.I.; Perova, N.V.; Shishatskaya, E.I.; Kalacheva, G.S.; Volova, T.G.

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13.

PHYSICOCHEMICAL PROPERTIES OF A PHOTOPROTEIN FROM THE HYDROID POLYP OBELIA-LONGISSIMA [Text] / V. S. BONDAR, K. P. TROFIMOV, E. S. VYSOTSKII // Biochem.-Moscow. - 1992. - Vol. 57, Is. 10. - P1020-1027. - Cited References: 36 . - 8. - ISSN 0006-2979

РУБ Biochemistry & Molecular Biology
Рубрики:
CALCIUM-ACTIVATED PHOTOPROTEINS
   CTENOPHORES MNEMIOPSIS SP
   BEROE-OVATA
   AEQUORIN
   CA-2+
   INDICATORS
   PROTEIN
   BINDING
   PURIFICATION
   EXTRACTION
Кл.слова (ненормированные):
BIOLUMINESCENCE -- CA2+-ACTIVATED PHOTOPROTEIN -- OBELIN -- CHROMATOGRAPHY -- CALCIUM
Аннотация: The photoprotein obelin was isolated and purified to homogeneity (as indicated by sodium dodecyl sulfate polyacrylamide gel electrophoresis) from hydroids of Obelia longissima by gel filtration on Sephadex G-75 fine, ion exchange chromatography on Polysil CA-300 (10 mum), hydrophobic chromatography on Phenyl-Sepharose CL-4B, gel filtration on Sephacryl S-200 superfine, ion exchange chromatography on a Mono Q column at pH 7.0, chromatofocusing on a Mono P column (pH gradient 6.0-4.0), and ion exchange chromatography on a Mono Q column at pH 5.5, 8.8, and 7.0. The molecular weight of the native protein was 30 kD, and that measured in the presence of SDS was 19.8 kD. The specific activity of obelin is 4.9.10(15) quanta/mg protein, pseudo-first-order constant of bioluminescence decay 4 sec-1, and quantum yield 0.16 The range of measurable Ca2+ concentrations is 10(-7) to 10(-5) M. The luminescence spectrum of obelin peaks at 469 nm, and the fluorescence emission maximum of the discharged protein is at 455 nm. The optimum pH for luminescence is between 9.0 and 10.5. The molecular ionization constants are pK1 6.8 and pK2 12.2, and the ionization constants for the active site are pK1 9.1 and pK2 10.2
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
BONDAR, V.S.; TROFIMOV, K.P.; VYSOTSKII, E.S.

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14.

Nanodiamond sorbents: New carriers for column chromatography of proteins [Text] / K. V. Purtov, A. P. Puzyr, V. S. Bondar // Dokl. Biochem. Biophys. - 2008. - Vol. 419, Is. 1. - P72-74, DOI 10.1134/S1607672908020075. - Cited References: 12 . - ISSN 1607-6729

РУБ Biochemistry & Molecular Biology + Biophysics

Держатели документа:
[Purtov, K. V.
Puzyr, A. P.
Bondar, V. S.] Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50
Доп.точки доступа:
Purtov, K.V.; Puzyr, A.P.; Bondar, V.S.

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15.

Meta-analysis of factors associated with omega-3 fatty acid contents of wild fish / M. I. Gladyshev [et al.] // Rev. Fish. Biol. Fish. - 2018. - Vol. 28, Is. 2. - P277-299, DOI 10.1007/s11160-017-9511-0. - Cited References:138. - The work was supported by a Russian Science Foundation Grant (No. 16-14-10001). . - ISSN 0960-3166. - ISSN 1573-5184

РУБ Fisheries + Marine & Freshwater Biology
Рубрики:
FATTY-ACID-COMPOSITION
DIETARY DOCOSAHEXAENOIC ACID
LONG-CHAIN
Кл.слова (ненормированные):
Docosahexaenoic acid -- Ecomorphological factors -- Eicosapentaenoic acid -- Nutritive value -- Phylogenetic factors
Аннотация: Fish are recognized as the main source of physiologically important omega-3 long-chain polyunsaturated fatty acids, namely, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), for human nutrition. However, muscle tissue contents of these fatty acids in diverse fish species, i.e., their nutritive value for humans, varied within two orders of magnitude. We reviewed contents of EPA and DHA, measured by similar methods using an internal standard during chromatography as mg per g of wet mass in 172 fish species belonging to 16 orders, to evaluate probable variations in phylogenetic and ecological drivers. EPA + DHA content varied from 25.6 mg g(-1) of wet mass (Sardinops sagax) to 0.12 mg g(-1) (Gymnura spp.). Multidimensional redundancy analysis revealed that among phylogenetic, ecomorphological and abiotic environmental factors, the highest proportion of variation contribution belonged to the shared contribution of sets of phylogenetic and ecomorphological factors. Specifically, the highest values of EPA + DHA content were characteristic of fish belonging to the orders Clupeiformes or Salmoniformes, were pelagic fast swimmers, ate zooplankton and inhabited marine waters or migrated from fresh to marine waters (anadromous migrations). High EPA and DHA content in muscle tissues of the above species appeared to be a metabolic adaptation for fast continuous swimming. In contrast to common beliefs, our meta-analysis did not support the significant influence of higher trophic levels (piscivory) and cold environments (homeoviscous adaptation) on EPA and DHA content in fish. However, many causes of high and low levels of physiologically important fatty acids in certain fish species remained unexplained and require evaluation in future studies.

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Держатели документа:
Russian Acad Sci, Fed Res Ctr, Krasnoyarsk Sci Ctr, Inst Biophys,Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Svobodny Av 79, Krasnoyarsk 660041, Russia.
Russian Acad Sci, AN Severtsov Inst Ecol & Evolut, Leninsky Prospect 33, Moscow 119071, Russia.
Lomonosov Moscow State Univ, Moscow 119899, Russia.

Доп.точки доступа:
Gladyshev, Michail I.; Sushchik, Nadezhda N.; Tolomeev, Alexander P.; Dgebuadze, Yury Yu; Russian Science Foundation [16-14-10001]

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16.

LC-MS and microscale NMR analysis of luciferin-related compounds from the bioluminescent earthworm Fridericia heliota / S. M. Marques [et al.] // Journal of Photochemistry and Photobiology B: Biology. - 2011. - Vol. 102, Is. 3. - P218-223, DOI 10.1016/j.jphotobiol.2010.12.006 . - ISSN 1011-1344
Кл.слова (ненормированные):
Bioluminescence -- Earthworm -- Fridericia heliota -- Luciferin -- Microscale NMR -- RP-HPLC-MS -- alkene -- alkyl group -- benzothiazole -- carboxylic acid -- hydroxyl group -- luciferin -- pterin -- absorption -- article -- bioluminescence -- earthworm -- Fridericia heliota -- isomer -- molecular weight -- nonhuman -- nuclear magnetic resonance -- priority journal -- reversed phase high performance liquid chromatography -- ultraviolet radiation -- Animals -- Chromatography, High Pressure Liquid -- Chromatography, Reverse-Phase -- Firefly Luciferin -- Luminescent Agents -- Magnetic Resonance Spectroscopy -- Mass Spectrometry -- Oligochaeta -- Fridericia heliota
Аннотация: This paper presents the main results of RP-HPLC-MS and microscale NMR analysis performed on Accompanying similar to Luciferin (AsLn(x)), compounds present in extracts of the bioluminescent earthworm Fridericia heliota that display similarities with Fridericia's luciferin, the substrate of the bioluminescent reaction. Three isomers of AsLn were discovered, AsLn(1), AsLn(2) and AsLn(3), all of which present a molecular weight of 529 Da. Their UV-Vis absorption spectra show maxima at 235 nm for AsLn(1), 238 and 295 nm for AsLn(2) and 241 and 295 nm for AsLn(3). MS n fragmentation patterns suggest the existence of carboxylic acid and hydroxyl moieties, and possibly chemical groups found in other luciferins like pterin or benzothiazole. The major isomer, AsLn(2), presents an aromatic ring and alkene and alkyl moieties. These luciferin-like compounds can be used as models that could give further insights into the structure of this newly discovered luciferin. В© 2010 Elsevier Inc. All rights reserved.

Scopus
Держатели документа:
Department of Chemistry and Biochemistry, Faculty of Sciences, Universidade Do Porto, Rua do Campo Alegre 687, 4169-007 Porto, Portugal
Laboratory of Photobiology, Institute of Biophysics, Russian Academy of Sciences, Akademgorodok, 660036 Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Marques, S.M.; Petushkov, V.N.; Rodionova, N.S.; Esteves Da Silva, J.C.G.

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17.

    Isolation, Study and Application of Organosolv Lignins / B. N. Kuznetsov [и др.] // J. Sib. Fed. Univ.-Chem. - 2016. - Vol. 9, Is. 4. - С. 454-482, DOI 10.17516/1998-2836-2016-9-4-454-482. - Cited References:137 . - ISSN 1998-2836. - ISSN 2313-6049
Рубрики:
SIZE-EXCLUSION CHROMATOGRAPHY
   MOLECULAR-WEIGHT DISTRIBUTION
Кл.слова (ненормированные):
organosolv lignin -- isolation -- structure -- catalytic depolymerization -- molecular weight -- application -- liquid hydrocarbons -- aerogels
Аннотация: The analysis of the literature on the methods of soluble organosolv lignins isolation, their physical-chemical study and on the method of their processing to porous aerogels and liquid hydrocarbons was carried out. A review of the literature allowed us to choice of the most important areas of research. For isolation from wood the soluble lignins free from sulfur the methods of catalytic peroxide delignification at mild conditions (temperature <= 100 degrees C, atmospheric pressure) and methods of lignin extraction by supercritical organic solvents were used. Molecular mass and molecular-mass distribution of ethanol-lignin samples isolated from aspenwood and abies-wood were studied by gel-permeation chromatography. Weighted molecular mass of ethanol-lignin from abies wood is 478 Da and that from aspen wood ethanol-lignin - 750 Da. Thus, the studied samples of ethanol-lignin have rather low molecular mass, what should facilitate their further processing to liquid hydrocarbons and aerogels. For the depolymerization of organosolv lignins to liquid hydrocarbons the processes of their catalytic conversion in supercritical alcohols have good prospects for the use. In the processes of lignin thermal conversion alcohols can to extract the products of lignin depolymerization and to alkylate these products, preventing their repolymerization to high molecular mass substances. To obtain a new class of nanoporous materials based on lignin the methods of organic aerogels synthesis from mixtures of lignin with other natural polymers and crosslinking agents were applied. It was found that the structure and properties of porous materials of aerogel type depend not only from the reaction mixture composition but from the method of drying. Drying in subcritical conditions leads to the formation of xerogels, in supercritical conditions - to the formation aerogels and the freezdrying - of cryogels. Obtained porous materials can have very low density (around 0.2 g/cm(3)), high specific surface area (to 500 m(2)/g) and the pore volume near 5 cm(3)/g.

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Держатели документа:
FRC Krasnoyarsk Sci Ctr SB RAS, Inst Chem & Chem Technol SB RAS, 50-24 Akademgorodok, Krasnoyarsk 660036, Russia.
FRC Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.
Univ Lorraine, CNRS, UMR 7198, Inst Jean Lamour,ENSTIB, 27 Rue Philippe Seguin,CS 60036, F-88026 Epinal, France.

Доп.точки доступа:
Kuznetsov, Boris N.; Malyar, Yuriy N.; Kuznetsova, Svetlana A.; Grishechko, Lyudmila I.; Kazachenko, Alexander S.; Levdansky, Alexander V.; Pestunov, Andrey V.; Boyandin, Anatoly N.; Celzard, Alan

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18.

In vitro and transdermal penetration of PHBV micro/nanoparticles [Text] / G. . Eke [et al.] // J. Mater. Sci.-Mater. Med. - 2014. - Vol. 25, Is. 6. - P1471-1481, DOI 10.1007/s10856-014-5169-5. - Cited References: 38. - The study was supported by the Government of the Russian Federation (Decree No. 220 of 09.04.2010) (Agreement No. 11.G34.31.0013) and (Grant No MD-3112.2012.4). We gratefully acknowledge the EC FP7 SKINTREAT project and the State Planning Organization (Turkey) for the grant to establish BIOMATEN. Mr. A. Buyuksungur is acknowledged for his contributions with CLSM. . - ISSN 0957-4530. - ISSN 1573-4838

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
DRUG-DELIVERY
   PLGA NANOPARTICLES
   CELLULAR UPTAKE
   MICROPARTICLES
   POLYHYDROXYALKANOATES
   CYTOTOXICITY
   SIZE
   POLYESTERS
   MECHANISM
   CELLS
Аннотация: The purpose of this study was to develop micro and nano sized drug carriers from poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and study the cell and skin penetration of these particles. PHBV micro/nanospheres were prepared by o/w emulsion method and were stained with a fluorescent dye, Nile Red. The particles were fractionated by centrifugation to produce different sized populations. Topography was studied by SEM and average particle size and its distribution were determined with particle sizer. Cell viability assay (MTT) was carried out using L929 fibroblastic cell line, and particle penetration into the cells were studied. Transdermal permeation of PHBV micro/nanospheres and tissue reaction were studied using a BALB/c mouse model. Skin response was evaluated histologically and amount of PHBV in skin was determined by gas chromatography-mass spectrometry. The average diameters of the PHBV micro/nanosphere batches were found to be 1.9 mu m, 426 and 166 nm. Polydispersity indices showed that the size distribution of micro sized particles was broader than the smaller ones. In vitro studies showed that the cells had a normal growth trend. MTT showed no signs of particle toxicity. The 426 and 166 nm sized PHBV spheres were seen to penetrate the cell membrane. The histological sections revealed no adverse effects. In view of this data nano and micro sized PHBV particles appeared to have potential to serve as topical and transdermal drug delivery carriers for use on aged or damaged skin or in cases of skin diseases such as psoriasis, and may even be used in gene transfer to cells.

WOS
Держатели документа:
[Eke, G.
Hasirci, N.
Hasirci, V.] Middle E Tech Univ, Dept Micro & Nanotechnol, TR-06800 Ankara, Turkey
[Eke, G.
Hasirci, N.
Hasirci, V.] METU Ctr Excellence Biomat & Tissue Engn, BIOMATEN, TR-06800 Ankara, Turkey
[Eke, G.] Ahi Evran Univ, Fac Arts & Sci, Dept Chem, TR-40100 Kirsehir, Turkey
[Kuzmina, A. M.
Shishatskaya, E. I.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Goreva, A. V.
Shishatskaya, E. I.] Inst Biophys SB RAS, Krasnoyarsk 66003, Russia
[Hasirci, N.
Hasirci, V.] Middle E Tech Univ, Dept Biotechnol, TR-06800 Ankara, Turkey
[Hasirci, N.
Hasirci, V.] Middle E Tech Univ, Dept Biomed Engn, TR-06800 Ankara, Turkey
[Hasirci, N.] Middle E Tech Univ, Dept Chem, TR-06800 Ankara, Turkey
[Hasirci, V.] Middle E Tech Univ, Dept Biol Sci, TR-06800 Ankara, Turkey
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Eke, G...; Kuzmina, A.M.; Goreva, A.V.; Shishatskaya, E.I.; Hasirci, N...; Hasirci, V...; Government of the Russian Federation [220, 11.G34.31.0013, MD-3112.2012.4]; EC FP7 SKINTREAT project; State Planning Organization (Turkey)

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19.

Extracellular Oxidases of Basidiomycete Neonothopanus nambi: Isolation and Some Properties / N. O. Ronzhin, O. A. Mogilnaya, K. S. Artemenko [et al.] // Dokl. Biochem. Biophys. - 2020. - Vol. 490, Is. 1. - P38-42, DOI 10.1134/S1607672920010135. - Cited References:15 . - ISSN 1607-6729. - ISSN 1608-3091

РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
PEROXIDASE-ACTIVITY
LIGHT-EMISSION
Кл.слова (ненормированные):
extracellular oxidases -- basidiomycete Neonothopanus nambi -- beta-glucosidase -- gel-filtration chromatography -- veratryl alcohol -- phenol -- FAD
Аннотация: Using the original technique of treating biomass with beta-glucosidase, a pool of extracellular fungal enzymes was obtained for the first time from the mycelium of basidiomycete Neonothopanus nambi. Two protein fractions containing enzymes with oxidase activity were isolated from the extract by gel-filtration chromatography and conventionally called F1 and F2. Enzyme F1 has a native molecular weight of 80-85 kDa and does not contain chromophore components; however, it catalyzes the oxidation of veratryl alcohol with K-m = 0.52 mM. Probably, this enzyme is an alcohol oxidase. Enzyme F2 with a native molecular weight of approximately 60 kDa is a FAD-containing protein. It catalyzes the cooxidation of phenol with 4-aminoantipyrine without the addition of exogenous hydrogen peroxide, which distinguishes it from the known peroxidases. It was assumed that this enzyme may be a mixed-function oxidase. F2 oxidase has K-m value 0.27 mM for phenol. The temperature optimums for oxidases F1 and F2 are 22-35 and 55-70 degrees C, and pH optimums are 6 and 5, respectively.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci, Inst Biophys,Fed Res Ctr, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.

Доп.точки доступа:
Ronzhin, N. O.; Mogilnaya, O. A.; Artemenko, K. S.; Posokhina, E. D.; Bondar, V. S.

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20.

Extracellular Oxidase from the Neonothopanus nambi Fungus as a Promising Enzyme for Analytical Applications / O. Mogilnaya, N. Ronzhin, E. Posokhina, V. Bondar // Protein J. - 2021, DOI 10.1007/s10930-021-10010-z. - Cited References:39. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences, Project No. 0356-2019-0022. . - Article in press. - ISSN 1572-3887. - ISSN 1573-4943

РУБ Biochemistry & Molecular Biology
Рубрики:
ARYL-ALCOHOL OXIDASE
   GLUCOSE-OXIDASE
   PEROXIDASES
   MECHANISM
Кл.слова (ненормированные):
Extracellular oxidase -- Flavoprotein -- Fungi -- Phenol
Аннотация: The extracellular enzyme with oxidase function was extracted from the Neonothopanus nambi luminescent fungus by using mild processing of mycelium with beta-glucosidase and then isolated by gel-filtration chromatography. The extracted enzyme is found to be a FAD-containing protein, catalyzing phenol co-oxidation with 4-aminoantipyrine without addition of H2O2, which distinguishes it from peroxidases. This fact allowed us to assume that this enzyme may be a mixed-function oxidase. According to gel-filtration chromatography and SDS-PAGE, the oxidase has molecular weight of 60 kDa. The enzyme exhibits maximum activity at 55-70 degrees C and pH 5.0. Kinetic parameters K-m and V-max of the oxidase for phenol were 0.21 mM and 0.40 mu M min(-1). We suggest that the extracted enzyme can be useful to develop a simplified biosensor for colorimetric detection of phenol in aqueous media, which does not require using hydrogen peroxide.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Fed Res Ctr,Krasnoyarsk Sci Ctr SB RAS, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Mogilnaya, Olga; Ronzhin, Nikita; Posokhina, Ekaterina; Bondar, Vladimir; [0356-2019-0022]

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