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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Бондарь В.С., Высоцкий Е.С., Гамалей И.А., Каулин А.Б.
Заглавие : Получение, свойства и применение кальцийчувствительного фотопротеина из гидроида Obelia longissima : научное издание
Место публикации : Цитология. - 1991. - Т. 33, N 6. - С. 50-59. - ISSN 0041-3771
ГРНТИ : 31.23.27
Предметные рубрики: ФОСФОПРОТЕИНЫ
КАЛЬЦИЙ ЧУВСТВИТЕЛЬНЫЙ
ВЫДЕЛЕНИЕ
ГИДРОЛИЗ
OBELIA LONGISSIMA
OBELIN
PHOSPHOMOTEIN
CHROMATOGRAPHY
Аннотация: С помощью Ca{2}{+} - активируемого протеина белина измеряли конц-ию свободного ионизированного кальция в макрофагах (МФ) в покое и при действии различных стимуляторов. Использовали обелин, выделенный из гидроида Obelia longissima и очищенный с использованием гельфильтрации на Сефадексе, ИОХ на полисиле, гидрофобной хр-фии на фенил-сефарозе, повторной гель-фильтрации на Сефадексе. Нагрузку МФ фотопротеином проводили модифицированным методом "осмотического лизиса пиносом". Фоновое значение конц-ии цитоплазматич. кальция [Ca{2}{+}][j] в покоящихся МФ, зарегистрированное с помощью обелина, соответствует 0,5-0,7 мкМ. Сыворотка крупного рогатого скота (20%) стимулирует периодич. импульсное повышение [Ca{2}{+}][j] до 1,3 мкМ с быстрым падением практически до уровня фона, наблюдаемое в течение не менее 30 мин, NaF (20 мМ), добавленный к суспензии МФ, вызывает быстрое однократное увеличение [Ca{2}{+}][j] до 1,4 мкМ. АТФ (30-60 мкМ) стимулирует повышение [Ca{2}{+}][j] в МФ до 1,5 мкМ. цАМФ (20 мкМ) повышает конц-ию свободного внутриклеточного кальция до 1,5 мкМ с быстрым падением и возвращением за 30 с к уровню фона, при этом ЭГТА (2 мМ) не снимает стимулирующего эффекта цАМФ на МФ. При изменении внутриклеточного рН МФ в щелочную сторону эффект цАМФ качественно сохраняется, однако повышение [Ca{2}{+}][j] менее выражено. Закисление цитоплазмы вызывает качественное и количеств. изменение кальциевого ответа МФ на добавку цАМФ: [Ca{2}{+}][j] не повышается более чем до 1,3 мкМ, время достижения макс. уровня [Ca{2}{+}][j] и возвращения к исходному фону увеличено. Рассматриваются возможные мех-мы действия различных стимуляторов на МФ, сопровождающиеся повышением уровня внутриклеточного свободного кальция. Библ. 38. Россия, Ин-т биофизики СОРАН, Красноярск.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : BONDAR V.S., TROFIMOV K.P., VYSOTSKII E.S.
Заглавие : PHYSICOCHEMICAL PROPERTIES OF A PHOTOPROTEIN FROM THE HYDROID POLYP OBELIA-LONGISSIMA
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1992. - Vol. 57, Is. 10. - С. 1020-1027. - 8. - ISSN 0006-2979
Примечания : Cited References: 36
Предметные рубрики: CALCIUM-ACTIVATED PHOTOPROTEINS
CTENOPHORES MNEMIOPSIS SP
BEROE-OVATA
AEQUORIN
CA-2+
INDICATORS
PROTEIN
BINDING
PURIFICATION
EXTRACTION
Ключевые слова (''Своб.индексиров.''): bioluminescence--ca2+-activated photoprotein--obelin--chromatography--calcium
Аннотация: The photoprotein obelin was isolated and purified to homogeneity (as indicated by sodium dodecyl sulfate polyacrylamide gel electrophoresis) from hydroids of Obelia longissima by gel filtration on Sephadex G-75 fine, ion exchange chromatography on Polysil CA-300 (10 mum), hydrophobic chromatography on Phenyl-Sepharose CL-4B, gel filtration on Sephacryl S-200 superfine, ion exchange chromatography on a Mono Q column at pH 7.0, chromatofocusing on a Mono P column (pH gradient 6.0-4.0), and ion exchange chromatography on a Mono Q column at pH 5.5, 8.8, and 7.0. The molecular weight of the native protein was 30 kD, and that measured in the presence of SDS was 19.8 kD. The specific activity of obelin is 4.9.10(15) quanta/mg protein, pseudo-first-order constant of bioluminescence decay 4 sec-1, and quantum yield 0.16 The range of measurable Ca2+ concentrations is 10(-7) to 10(-5) M. The luminescence spectrum of obelin peaks at 469 nm, and the fluorescence emission maximum of the discharged protein is at 455 nm. The optimum pH for luminescence is between 9.0 and 10.5. The molecular ionization constants are pK1 6.8 and pK2 12.2, and the ionization constants for the active site are pK1 9.1 and pK2 10.2
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J.
Заглавие : The yellow bioluminescence bacterium, Vibrio fischeri Y1, contains a bioluminescence active riboflavin protein in addition to the yellow fluorescence FMN protein
Место публикации : Biochemical and Biophysical Research Communications. - 1995. - Vol. 211, Is. 3. - С. 774-779. - ISSN 0006291X (ISSN) , DOI 10.1006/bbrc.1995.1880
Ключевые слова (''Своб.индексиров.''): riboflavin--article--bioluminescence--fluorescence--nonhuman--priority journal--protein analysis--protein synthesis--vibrio--vibrionaceae--bacterial proteins--chromatography, gel--chromatography, thin layer--flavin mononucleotide--flavoproteins--luminescence--riboflavin--spectrometry, fluorescence--support, u.s. gov't, p.h.s.--vibrio--bacteria (microorganisms)--photobacterium--vibrio--vibrio fischeri
Аннотация: The yellow bioluminescence Y1 strain of Vibrio fischeri can produce a 22 kDa protein with either FMN or riboflavin as a bound fluorophore. Both forms are active for shifting the bioluminescence spectral maximum. The fluorescence spectral distribution of the two proteins differs slightly and the in vivo emission appears to be an equal mixture of the two. The bioluminescence activity of the riboflavin Y1 protein contrasts with the inactivity of the related Photobacterium type.
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Kalachova G.S., Guseynova V.E.
Заглавие : Rapid assay of fatty acid composition using a portable high-performance liquid chromatograph for monitoring aquatic ecosystems
Место публикации : Journal of Chromatography A. - 1995. - Vol. 695, Is. 2. - С. 223-228. - ISSN 00219673 (ISSN) , DOI 10.1016/0021-9673(94)01090-2
Ключевые слова (''Своб.индексиров.''): fatty acid--alga--article--culture medium--ecology--high performance liquid chromatography--instrument--methodology--priority journal--ultraviolet spectrophotometry--water analysis
Аннотация: The chromatographic conditions presented allowed the separation of the nitrophenacyl derivatives of standards of eleven free fatty acids (FFA) using a portable high-performance chromatograph, suitable for use aboard a research vessel. A statistically significant linear correlation between UV absorbance and amount of the analytes injected was obtained. The method was tested on FFA from algae cultural media. The method can be used for the ecological monitoring of natural waters.
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tyulkova N.A., Sandalova T.P.
Заглавие : Comparative study of temperature effects on bacterial luciferases
Колич.характеристики :10 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1996. - Vol. 61, Is. 2. - P205-214. - ISSN 0006-2979
Примечания : Cited References: 23
Предметные рубрики: BIOLUMINESCENCE
Ключевые слова (''Своб.индексиров.''): bacterial luciferase--temperature--activation energy
Аннотация: Effects of temperature on bioluminescent patterns of luciferases from luminescent bacteria Vibrio harveyi, Vibrio fischeri, Photobacterium leiognathi, and Photobacterium phosphoreum were studied. The highest luminescence level was observed at 15-25 degrees C for the luciferase from P. phosphoreum, at 20-30 degrees C for the V. fischeri and P. leiognathi enzymes, and at 30-37 degrees C for the enzyme from V. harveyi. All the luciferases were significantly stabilized at increased salt concentrations, at low pH values, or in the presence of dithiothreitol (DTT) and EDTA. The addition of DTT and EDTA affected the reversible stage of enzyme inactivation, while salts reduced the rate of the irreversible stage. A peak corresponding to aggregated protein was detected by gel chromatography of irreversibly inactivated luciferase. Activation energies were calculated for each luciferase in bioluminescent reactions with decanal, dodecanal, tetradecanal, and without aldehydes. The activation energy of the reaction with tetradecanal was much lower than those with the other aldehydes. The temperature dependence of the lifetime of the long-lived reaction intermediate showed that in the 10-30 degrees C interval all the luciferases, except for the enzyme from V. harveyi, have only one active form.
WOS
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Frank L.A., Illarionova V.A., Vysotski E.S.
Заглавие : Use of proZZ-obelin fusion protein in bioluminescent immunoassay
Место публикации : Biochem. Biophys. Res. Commun.: ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS, 1996. - Vol. 219, Is. 2. - С. 475-479. - 5. - ISSN 0006-291X, DOI 10.1006/bbrc.1996.0258
Примечания : Cited References: 21
Предметные рубрики: ESCHERICHIA-COLI
EXPRESSION
AEQUORIN
PURIFICATION
SYSTEM
Аннотация: Obelin is a photoprotein that emits light by Ca2+-binding. To develop a bioluminescent immunoassay based on the light emission property of obelin, we have expressed the apoobelin fusion protein with ZZ-domain of S. aureus protein A in E. coil by recombinant DNA techniques. The pro2Z-obelin expressed was purified by one-step affinity chromatography on IgG-Agarose. The purified proZZ-obelin has both the luminescent activity of obelin and the IgG-binding ability of ZZ-domain. The specific activity of fusion protein was 8.5 x 10(15) photons per mg of protein. (C) 1996 Academic Press, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sevastianov V.I., Perova N.V., Shishatskaya E.I., Kalacheva G.S., Volova T.G.
Заглавие : Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood
Колич.характеристики :14 с
Место публикации : J. Biomater. Sci.-Polym. Ed.: VSP BV, 2003. - Vol. 14, Is. 10. - P1029-1042. - ISSN 0920-5063, DOI 10.1163/156856203769231547
Примечания : Cited References: 34
Предметные рубрики: BIODEGRADABLE POLYESTERS
POLYMERS
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates (phas)--polyhydroxybutyrate (phb)--poly(hydroxybutyrate-co-hydroxyvalerate) (phbv)--endotoxins--beta-hydroxy acids--hemocompatibility
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C-16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only beta-OH-C-14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.
WOS
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Rodionova N.S., Bondar' V.S.
Заглавие : Study of the luminescence system of the soil enchytraeid Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae)
Место публикации : Doklady Biochemistry and Biophysics. - 2003. - Vol. 391, Is. 1-6. - С. 204-207. - ISSN 16076729 (ISSN) , DOI 10.1023/A:1025105323648
Ключевые слова (''Своб.индексиров.''): adsorption chromatography--animal cell--annelid worm--article--luminescence--nonhuman--purification--animal--drug antagonism--isolation and purification--metabolism--physiology--soil--animalia--annelida--clitellata--enchytraeidae--edetic acid--luciferase--magnesium--animals--edetic acid--luciferases--luminescent measurements--magnesium--oligochaeta--soil
Scopus
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sevastianov V.I., Perova N.V., Shishatskaya E.I., Kalacheva G.S., Volova T.G.
Заглавие : Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - С. 1029-1042. - ISSN 09205063 (ISSN) , DOI 10.1163/156856203769231547
Ключевые слова (''Своб.индексиров.''): ?-hydroxy acids--endotoxins--hemocompatibility--poly(hydroxybutyrate-co-hydroxyvalerate) (phbv)--polyhydroxyalkanoates (phas)--polyhydroxybutyrate (phb)--bacterium lipopolysaccharide--carbon--complement--copolymer--hydroxyacid--long chain fatty acid--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--valeric acid derivative--adult--article--biofilm--biotechnology--blood analysis--blood clotting--blood compatibility--cell function--chemical analysis--chemical composition--complement activation--concentration (parameters)--controlled study--gas chromatography--hemostasis--human--human cell--mass spectrometry--micromorphology--nonhuman--priority journal--purification--quantitative analysis--sampling--synthesis--thrombocyte adhesion--wautersia eutropha--biocompatible materials--blood--blood coagulation tests--chromatography, gas--complement activation--cupriavidus necator--fatty acids--humans--platelet adhesiveness--polyesters--surface properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.
Scopus
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Makhutova O.N., Sushchik N.N., Kalacheva G.S.
Заглавие : The value of fatty acid composition of tricylglycerols and polar lipids of seston at analysis of food spectra of microzooplancton in small reservoir Buguch
Место публикации : Doklady Akademii Nauk. - 2004. - Vol. 395, Is. 4. - С. 562-565. - ISSN 08695652 (ISSN)
Ключевые слова (''Своб.индексиров.''): algae--bacteria--chromatographic analysis--chromatography--organic compounds--food spectra--microzooplancton--polar lipids--microorganisms
Аннотация: Food spectrum of microzooplancton in fresh-water ecological systems is lowly studied area. However, these organisms play very important role in transfer of substance and energy in water reservoirs. Traditional method of analysis of food in the microzooplancton does not makes it possible to determine real assimilation of organic substance. Therefore, biochemical methods have been developed which are based on analysis of compositions of fatty acids of organic substance. This leads to determining of the food assimilation. Fatty-acid compositions of lipids allow specialists to determine specific groups of organisms such as bacteria, microalgae etc. Data of the analysis are presented.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondareva L.G., Kalyakina O.P., Bolsunovskii A.Ya.
Заглавие : Effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system as studied by dual-column ion chromatography and ?-ray spectrometry
Место публикации : Journal of Analytical Chemistry. - 2006. - Vol. 61, Is. 4. - С. 354-358. - ISSN 10619348 (ISSN) , DOI 10.1134/S1061934806040101
Ключевые слова (''Своб.индексиров.''): absorption--chromatographic analysis--complexation--gamma ray spectrometers--radioisotopes--?-ray spectrometry--absorption-release processes--dual-column ion chromatography--humic acid--organic acids
Аннотация: The effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system was studied by dual-column ion chromatography and ?-ray spectrometry. With the use of ion chromatography, it was found that processes related to the absorption of SO 42- and Cl- anions by a solid phase with the release of NO 3- , PO 43- , and F- to a liquid phase competed in the test systems as the concentration of water-soluble organic carbon (WSOC) was increased. Only the test anions were released in the systems without the introduction of an additional amount of WSOC as humic acid. With the use of ?-ray spectrometry, it was found that the release of 60Co, 152Eu, and 241Am radionuclides to the liquid phase in the systems with added humic acid began much earlier than in the system without the addition of humic acid. In this case, the amount of released radionuclides was greater than the amount of radioisotopes released in the system without the addition of humic acid: ?25% 241Am, ?3% 152Eu, and ?0.8% 60Co in the system with added humic acid or 0.8% 152Eu and <0.1% 60Co in the system without the addition of humic acid. The 241Am radionuclide was not determined in the system without the addition of humic acid. An increase in the concentration of WSOC in the experimental system of bottom sediments-Yenisei river water initiated the release of 60Co, 152Eu, and 241Am anthropogenic radionuclides from bottom sediments because of the formation of soluble complexes capable of migration. An increase in the concentration of WSOC had almost no effect on the release of 40K and 137Cs radionuclides. В© Pleiades Publishing, Inc., 2006.
Scopus
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondareva L.G., Kalyakina O.P., Bolsunovskii A.Y.
Заглавие : Effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system as studied by dual-column ion chromatography and gamma-ray spectrometry
Место публикации : J. Anal. Chem.: MAIK NAUKA/INTERPERIODICA, 2006. - Vol. 61, Is. 4. - С. 354-358. - 5. - ISSN 1061-9348, DOI 10.1134/S1061934806040101
Примечания : Cited References: 11
Аннотация: The effect of humic acid on absorption-release processes in the bottom sediments-Yenisei river water system was studied by dual-column ion chromatography and gamma-ray spectrometry. With the use of ion chromatography, it was found that processes related to the absorption of SO42- and Cl- anions by a solid phase with the release of NO3-, PO43-, and F- to a liquid phase competed in the test systems as the concentration of water-soluble organic carbon (WSOC) was increased. Only the test anions were released in the systems without the introduction of an additional amount of WSOC as humic acid. With the use of gamma-ray spectrometry, it was found that the release of Co-60, Eu-152, and Am-241 radionuclides to the liquid phase in the systems with added humic acid began much earlier than in the system without the addition of humic acid. In this case, the amount of released radionuclides was greater than the amount of radioisotopes released in the system without the addition of humic acid: similar to 25% Am-241, similar to 3% Eu-152, and similar to 0.8% Co-60 in the system with added humic acid or 0.8% Eu-152 and 0.1% Co-60 in the system without the addition of humic acid. The Am-241 radionuclide was not determined in the system without the addition of humic acid. An increase in the concentration of WSOC in the experimental system of bottom sediments-Yenisei river water initiated the release of Co-60, Eu-152, and Am-241 anthropogenic radionuclides from bottom sediments because of the formation of soluble complexes capable of migration. An increase in the concentration of WSOC had almost no effect on the release of K-40 and Cs-137 radionuclides.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Rodionova N.S.
Заглавие : Purification and partial spectral characterization of a novel luciferin from the luminous enchytraeid Fridericia heliota
Место публикации : Journal of Photochemistry and Photobiology B: Biology. - 2007. - Vol. 87, Is. 2. - С. 130-136. - ISSN 10111344 (ISSN) , DOI 10.1016/j.jphotobiol.2007.03.006
Ключевые слова (''Своб.индексиров.''): atp--bioluminescence--earthworms--enchytraeid--luciferase--luciferin--adenosine triphosphate--luciferase--luciferin--animal cell--anion exchange chromatography--annelid worm--article--controlled study--earthworm--firefly--luminance--luminescence--nonhuman--priority journal--protein analysis--protein purification--radiation absorption--reversed phase liquid chromatography--ultraviolet radiation--adenosine triphosphate--animals--luciferases--luminescent agents--oligochaeta--spectrum analysis--enchytraeidae--fridericia heliota
Аннотация: A homogeneous luciferin preparation has been obtained from the luminous soil enchytraeid Fridericia heliota, which has an ATP-dependent luminescent system. A procedure for luciferin purification without losing fractions of active luciferase has been developed. The luciferin specific activity is 4000 times increased; its UV absorption spectrum maximum is 294 nm with a local minimum at 262 nm. The luciferin of the enchytraeid F. heliota is significantly different from firefly luciferin, whose luminescent reaction also requires ATP, and it also appears to have no similarities to other known luciferins. В© 2007 Elsevier B.V. All rights reserved.
Scopus
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Purtov K.V., Puzyr A.P., Bondar V.S.
Заглавие : Nanodiamond sorbents: New carriers for column chromatography of proteins
Колич.характеристики :3 с
Место публикации : Dokl. Biochem. Biophys.: SPRINGER, 2008. - Vol. 419, Is. 1. - С. 72-74. - ISSN 1607-6729, DOI 10.1134/S1607672908020075
Примечания : Cited References: 12
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Frank L.A., Borisova V.V., Vereshchagina T.A., Fomenko E.V., Anshits A.G., Gitelson I.I.
Заглавие : Affine magnetic sorbents supported on coal ash microspheres for recombinant protein isolation
Место публикации : Applied Biochemistry and Microbiology. - 2009. - Vol. 45, Is. 2. - С. 215-220. - ISSN 00036838 (ISSN) , DOI 10.1134/S0003683809020173
Ключевые слова (''Своб.индексиров.''): clytia gregaria--obelia longissima
Аннотация: The results of the development and utilization of an affine magnetic sorbent with Ni2+ ions immobilized on coal ash microspheres are reported. The applicability of the material in the isolation of Histag proteins is demonstrated by examples of the recombinant green fluorescent protein from Clytia gregaria and the Ca2+ regulated photoprotein obelin from Obelia longissima. The specific sorption capacity of the sorbent was 2-7 mg/cm3 for medium-size proteins (20-30 kDa). The particles are suitable for chromatography with the presence of chaotropic agents and EDTA. They are easy to manipulate as isolation of a target protein takes 30-35 min. On the one hand, the elevated affinity of the sorbent to proteins rich in native histidines may result in a high degree of irreversible sorption; on the other hand, it allows isolation of such proteins without the introduction of artificial polyhistidine fragments. В© 2009 Pleiades Publishing, Ltd.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kalacheva G.S., Sushchik N.N., Gladyshev M.I., Makhutova O.N.
Заглавие : Seasonal dynamics of fatty acids in the lipids of water moss Fontinalis antipyretica from the Yenisei River
Место публикации : Russian Journal of Plant Physiology. - 2009. - Vol. 56, Is. 6. - С. 795-807. - ISSN 10214437 (ISSN) , DOI 10.1134/S1021443709060090
Ключевые слова (''Своб.индексиров.''): acetylenic acids--fatty acids--fontinalis antipyretica--seasonal dynamics--bryophyta--fontinalis antipyretica
Аннотация: Identification of lipid fatty acids (FA) and studying of their seasonal dynamics in water moss Fontinalis antipyretica from the Yenisei River were carried out by means of gas chromatography-mass spectrometry. FA composition of water moss was notable for a relatively low level of saturated acids and predominance of polyunsaturated acids (PUFA) with double bonds (accounting for more than 30% of total FA) and polyunsaturated acids with double and triple bonds (acetylenic acids, accounting for more than 40% of total FA). Among PUFA, ?- and ?-linolenic (18:3?3 and 18:3?6), arachidonic (20:4?6), and eicosapentaenoic (20:5?3) acids prevailed. Relative content of PUFA from ?3-family was the greatest in spring, and the level of PUFA from ?6-group was essentially the same throughout all the seasons. In the biomass of water moss, we identified seven acetylenic acids; among them octadeca-9,12-dien-6-ynoic (6a,9,12-18:3), octadeca-9,12,15-trien-6-ynoic (6a,9,12,15-18:4), and eicosa-11,14-dien-8-ynoic (8a,11,14-20:3) acids were predominant. For the first time, in the lipids of water moss we identified an acetylenic eicosa-11,14,17-trien-8-ynoic acid (8a,11,14,15-20:4). Relative content of acetylenic acids in the total FA was great throughout the entire period of investigation with the peak accumulation in summer. Owing to a steadily high level in the biomass of water moss and the lack of other producers of these acids in the ecosystem, acetylenic FA are highly specific biochemical markers useful for the investigation of trophic interactions between higher aquatic plants and zoobenthos. В© Pleiades Publishing, Ltd., 2009.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Makhutova O.N., Kalachova G.S.
Заглавие : Content of essential polyunsaturated fatty acids in three canned fish species
Место публикации : International Journal of Food Sciences and Nutrition. - 2009. - Vol. 60, Is. 3. - С. 224-230. - ISSN 09637486 (ISSN) , DOI 10.1080/09637480701664761
Ключевые слова (''Своб.индексиров.''): canned fish--essential polyunsaturated fatty acids--herring--saury--sprat--docosahexaenoic acid--essential fatty acid--icosapentaenoic acid--linoleic acid--linolenic acid--oleic acid--polyunsaturated fatty acid--article--baltic sprat--canned food--controlled study--fatty acid analysis--fish--food analysis--nonhuman--pacific herring--pacific saury--russian federation--species difference--animals--fatty acids, unsaturated--fish products--fishes--food handling--food, preserved--gas chromatography-mass spectrometry--russia--clupea harengus--clupea pallasi--clupeidae--cololabis saira--sprattus sprattus--sprattus sprattus balticus
Аннотация: Three canned fish species - Pacific saury (Cololabis saira), Pacific herring (Clupea harengus) and Baltic sprat (Sprattus sprattus) - most common and popular in Russia, were analyzed for fatty acids. Special attention was paid to long-chain essential polyunsaturated fatty acids: eicosapentaenoic acid (20:5?3) and docosahexaenoic acid (22:6?3). Sums of eicosapentaenoic acid and docosahexaenoic acid in saury, herring and sprat were, on average, 2.42, 1.80 and 1.43 g/100 g product, respectively. Contents of these essential acids in all the canned fish species were found to be very high compared with many other fish reported in the available literature. All the canned fish appeared to be highly valuable products for human nutrition concerning the content of eicosapentaenoic and docosahexaenoic acids.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E., Goreva A., Kalacheva G., Volova T.
Заглавие : Biocompatibility and resorption of intravenously administered polymer microparticles in tissues of internal organs of laboratory animals
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2011. - Vol. 22, Is. 16. - С. 2185-2203. - ISSN 09205063 (ISSN) , DOI 10.1163/092050610X537138
Ключевые слова (''Своб.индексиров.''): 14c products of polymer resorption--intravenous administration--microparticles--resorbable polyhydroxybutyrate--tissues of internal organs--3-hydroxybutyric acid--sup14/supc products of polymer resorption--adverse effect--drug-delivery systems--growth and development--high molecular weight--initial molecular weight--initial values--internal organs--intravenous administration--laboratory animals--local response--matrix--mean diameter--micro-particles--polymer degradation--polymer microparticles--preparation process--radioactivity level--residual content--resorbable--sustained-release--tail veins--wistar rat--animals--biocompatibility--degradation--histology--molecular weight--morphology--radiation--radioactivity--rats--tissue--poly(3 hydroxybutyric acid)--animal experiment--animal tissue--article--biocompatibility--concentration process--controlled study--degradation--drug delivery system--female--gel permeation chromatography--heart--kidney parenchyma--liver--lung parenchyma--molecular weight--nonhuman--organ culture--priority journal--radioactivity--rat--spleen--animals--animals, laboratory--biocompatible materials--drug delivery systems--female--infusions, intravenous--materials testing--microspheres--molecular weight--particle size--polymers--rats--rats, wistar--tissue distribution--animalia--rattus--rattus norvegicus
Аннотация: Specimens of 14C-labeled polymer of 3-hydroxybutyric acid, P(3-HB), with different initial molecular weights, were used to prepare microparticles, whose morphology was not influenced by the M w of the polymer. During the particle preparation process, P(3-HB) molecular weight decreased by 15-20%. Sterile microparticles (mean diameter 2.4 ?m) were injected into the tail veins of Wistar rats (5 mg/rat). The effects of the particles administered to rats were studied based on the general response of animals and local response of internal organ tissues and blood morphology; no adverse effects on growth and development of the animals or unfavorable changes in the structure of the tissues of internal organs were observed. Measurements of radioactivity in tissues showed that 14C concentrations are different in different organs, changing during the course of the experiment. The main targets for 14C-labeled microparticles were tissues of the liver, spleen, and kidneys. Comparison of radioactivity levels and residual contents of high-molecular-weight matrix in tissues suggested that the most rapid metabolism and degradation of P(3-HB) occurred in the liver and spleen. Gel-permeation chromatography showed that at 3 h after the microparticles were injected into the bloodstream, polymer degradation started in all examined organs, except the lungs; at 12 weeks, the M w of the polymer matrix was as low as 20-30% of its initial value. The presence of high-molecular-weight (undegraded) polymer in the tissues at 12 weeks after administration of the particles suggests that P(3-HB) is degraded in tissues of internal organs slowly and, hence, P(3-HB)-based microparticles can be used as sustained-release drug-delivery systems. В© 2011 VSP.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Semenchenko V.P., Dubovskaya O.P., Fefilova E.B., Makhutova O.N., Buseva Z.F., Sushchik N.N., Razlutskij V.I., Lepskaya E.V., Baturina M.A., Kalachova G.S., Kononova O.N.
Заглавие : Effect of temperature on contents of essential highly unsaturated fatty acids in freshwater zooplankton
Место публикации : Limnologica. - 2011. - Vol. 41, Is. 4. - С. 339-347. - ISSN 00759511 (ISSN) , DOI 10.1016/j.limno.2011.03.001
Ключевые слова (''Своб.индексиров.''): cladocerans--copepods--essential polyunsaturated fatty acids--freshwater zooplankton--water temperature--biomass--climate effect--community structure--fatty acid--freshwater ecosystem--gas chromatography--lake ecosystem--multivariate analysis--seston--taxonomy--temperature effect--trophic level--zooplankton--animalia--copepoda--crustacea
Аннотация: In 11 lakes from cold and warm regions we studied the content of highly unsaturated fatty acids (HUFA) in seston (130?m) and crustacean zooplankton using gas chromatography-mass spectrometry. An increase of temperature correlated with a decrease of HUFA content in zooplankton. A multivariate canonical correlation analyses revealed, that the decrease of HUFA content was related with a decrease of per cent of copepods in zooplankton communities, which are known to have higher HUFA levels in their biomass, than cladocerans. This means that temperature primarily affected the HUFA levels indirectly, via changing of taxonomic structure of zooplankton community, while the homeoviscous adaptation of zooplankton individuals had comparatively lower importance. As found, water temperature was better predictor of HUFA contents of zooplankton, than the fatty acid composition of seston. Thus, it can be predicted, that a probable climate warming will decrease the content of the essential HUFA in freshwater zooplankton with possible negative consequences for animals of higher trophic levels. В© 2011 Elsevier GmbH.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Marques S.M., Petushkov V.N., Rodionova N.S., Esteves Da Silva J.C.G.
Заглавие : LC-MS and microscale NMR analysis of luciferin-related compounds from the bioluminescent earthworm Fridericia heliota
Место публикации : Journal of Photochemistry and Photobiology B: Biology. - 2011. - Vol. 102, Is. 3. - С. 218-223. - ISSN 10111344 (ISSN) , DOI 10.1016/j.jphotobiol.2010.12.006
Ключевые слова (''Своб.индексиров.''): bioluminescence--earthworm--fridericia heliota--luciferin--microscale nmr--rp-hplc-ms--alkene--alkyl group--benzothiazole--carboxylic acid--hydroxyl group--luciferin--pterin--absorption--article--bioluminescence--earthworm--fridericia heliota--isomer--molecular weight--nonhuman--nuclear magnetic resonance--priority journal--reversed phase high performance liquid chromatography--ultraviolet radiation--animals--chromatography, high pressure liquid--chromatography, reverse-phase--firefly luciferin--luminescent agents--magnetic resonance spectroscopy--mass spectrometry--oligochaeta--fridericia heliota
Аннотация: This paper presents the main results of RP-HPLC-MS and microscale NMR analysis performed on Accompanying similar to Luciferin (AsLn(x)), compounds present in extracts of the bioluminescent earthworm Fridericia heliota that display similarities with Fridericia's luciferin, the substrate of the bioluminescent reaction. Three isomers of AsLn were discovered, AsLn(1), AsLn(2) and AsLn(3), all of which present a molecular weight of 529 Da. Their UV-Vis absorption spectra show maxima at 235 nm for AsLn(1), 238 and 295 nm for AsLn(2) and 241 and 295 nm for AsLn(3). MS n fragmentation patterns suggest the existence of carboxylic acid and hydroxyl moieties, and possibly chemical groups found in other luciferins like pterin or benzothiazole. The major isomer, AsLn(2), presents an aromatic ring and alkene and alkyl moieties. These luciferin-like compounds can be used as models that could give further insights into the structure of this newly discovered luciferin. В© 2010 Elsevier Inc. All rights reserved.
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