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Study of Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing / T. G. Volova, N. A. Voinov // Prikladnaia biokhimiia i mikrobiologiia. - 2004. - Vol. 40, Is. 3. - С. 296-300 . - ISSN 0555-1099
Кл.слова (ненормированные):
carbon monoxide -- coal -- polyester -- antibiotic resistance -- article -- culture medium -- enzyme specificity -- gas -- growth, development and aging -- metabolism -- microbiology -- Wautersia eutropha -- Carbon Monoxide -- Coal -- Culture Media -- Cupriavidus necator -- Drug Resistance, Microbial -- Gases -- Polyesters -- Substrate Specificity
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange have been studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of the substrate utilization (up to 90%).

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division of the Russian Academy of Sciences, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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Study of a Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing [Text] / T. G. Volova, N. A. Voinov // Appl. Biochem. Microbiol. - 2004. - Vol. 40, Is. 3. - P. 249-252, DOI 10.1023/B:ABIM.0000025946.47013.03. - Cited References: 18 . - ISSN 0003-6838

РУБ Biotechnology & Applied Microbiology + Microbiology

Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange were studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of substrate utilization (up to 90%).

WOS
Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
Siberian State Technol Univ, Minist Educ Russian Federat, Krasnoyarsk 660049, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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Study of a Ralstonia eutropha culture producing polyhydroxyalkanoates on products of coal processing / T. G. Volova, N. A. Voinov // Applied Biochemistry and Microbiology. - 2004. - Vol. 40, Is. 3. - P249-252, DOI 10.1023/B:ABIM.0000025946.47013.03 . - ISSN 0003-6838
Кл.слова (ненормированные):
alkanoic acid -- carbon monoxide -- coal -- hydrogen -- lignite -- algorithm -- article -- bacterial strain -- bacterium culture -- coal gasification -- controlled study -- gas -- gas exchange -- mathematical computing -- nonhuman -- Ralstonia eutropha -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: Kinetic indices of growth, polyhydroxyalkanoate (PHA) accumulation, and gas exchange were studied in a culture of the carbon monoxide-resistant hydrogen strain Ralstonia eutropha B-5786 grown on a gaseous substrate (GS) obtained by lignite gasification. The GS was shown to be suitable for PHA production. To increase the degree of GS consumption, various modes of gas supply to the culture were tested. Based on the results, an algorithm was developed for calculating and controlling gas-exchange parameters in the PHA-accumulating culture of Ralstonia eutropha, grown on a new GS allowing high polymer yields (up to 75%) and degrees of substrate utilization (up to 90%).

Scopus
Держатели документа:
Institute of Biophysics, Siberian Div. Russ. Acad. of Sci., Krasnoyarsk, 660036, Russian Federation
Siberian State Technol. University, Min. of Educ. of the Russ. Fed., Krasnoyarsk, 660049, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Voinov, N.A.

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Ni2+-zeolite/ferrosphere and Ni2+-silica/ferrosphere beads for magnetic affinity separation of histidine-tagged proteins / T. A. Vereshchagina [et al.] // Dalton Trans. - 2016. - Vol. 45, Is. 4. - P1582-1592, DOI 10.1039/c5dt03827h . - ISSN 1477-9226
Кл.слова (ненормированные):
Alkalinity -- Amino acids -- Chemical modification -- Fly ash -- Hydrothermal synthesis -- Ion exchange -- Magnetic separation -- Magnetism -- Proteins -- Silica -- Zeolites -- Conventional methods -- Core shell structure -- Green fluorescent protein -- Histidine-tagged proteins -- Hydrothermal treatments -- Magnetic affinity -- Mesoporous Silica -- Sorption capacities -- Nickel
Аннотация: Magnetic Ni2+-zeolite/ferrosphere and Ni2+-silica/ferrosphere beads (Ni-ferrosphere beads - NFB) of a core-shell structure were synthesized starting from coal fly ash ferrospheres having diameters in the range of 0.063-0.050 mm. The strategy of NFB fabrication is an oriented chemical modification of the outer surface preserving the magnetic core of parent beads with the formation of micro-mesoporous coverings. Two routes of ferrosphere modification were realized, such as (i) hydrothermal treatment in an alkaline medium resulting in a NaP zeolite layer and (ii) synthesis of micro-mesoporous silica on the glass surface using conventional methods. Immobilization of Ni2+ ions in the siliceous porous shell of the magnetic beads was carried out via (i) the ion exchange of Na+ for Ni2+ in the zeolite layer or (ii) deposition of NiO clusters in the zeolite and silica pores. The final NFB were tested for affinity in magnetic separation of the histidine-tagged green fluorescent protein (GFP) directly from a cell lysate. Results pointed to the high affinity of the magnetic beads towards the protein in the presence of 10 mM EDTA. The sorption capacity of the ferrosphere-based Ni-beads with respect to GFP was in the range 1.5-5.7 mg cm-3. © The Royal Society of Chemistry.

Scopus,
WOS
Держатели документа:
Institute of Chemistry and Chemical Technology SB RAS, 50/24 Akademgorodok, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodnyi Avenue, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Vereshchagina, T. A.; Fedorchak, M. A.; Sharonova, O. M.; Fomenko, E. V.; Shishkina, N. N.; Zhizhaev, A. M.; Kudryavtsev, A. N.; Frank, L. A.; Anshits, A. G.

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Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide [Text] / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Appl. Microbiol. Biotechnol. - 2002. - Vol. 58, Is. 5. - P. 675-678, DOI 10.1007/s00253-002-0941-8. - Cited References: 35 . - ISSN 0175-7598

РУБ Biotechnology & Applied Microbiology
Рубрики:
PSEUDOMONAS-OLEOVORANS
   ALCALIGENES-EUTROPHUS
   COAL
   CULTIVATION
   METABOLISM
   SUBSTRATE
   CULTURE
   CO2
   H-2
   O-2
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxyalkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (beta-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly beta-hydroxybutyrate (more than 99 mol%) with trace amounts of beta-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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Affine magnetic sorbents supported on coal ash microspheres for recombinant protein isolation / L. A. Frank [et al.] // Applied Biochemistry and Microbiology. - 2009. - Vol. 45, Is. 2. - P215-220, DOI 10.1134/S0003683809020173 . - ISSN 0003-6838
Кл.слова (ненормированные):
Clytia gregaria -- Obelia longissima
Аннотация: The results of the development and utilization of an affine magnetic sorbent with Ni2+ ions immobilized on coal ash microspheres are reported. The applicability of the material in the isolation of Histag proteins is demonstrated by examples of the recombinant green fluorescent protein from Clytia gregaria and the Ca2+ regulated photoprotein obelin from Obelia longissima. The specific sorption capacity of the sorbent was 2-7 mg/cm3 for medium-size proteins (20-30 kDa). The particles are suitable for chromatography with the presence of chaotropic agents and EDTA. They are easy to manipulate as isolation of a target protein takes 30-35 min. On the one hand, the elevated affinity of the sorbent to proteins rich in native histidines may result in a high degree of irreversible sorption; on the other hand, it allows isolation of such proteins without the introduction of artificial polyhistidine fragments. В© 2009 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation
Institute of Chemistry and Chemical Technology, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660049, Russian Federation
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Frank, L.A.; Borisova, V.V.; Vereshchagina, T.A.; Fomenko, E.V.; Anshits, A.G.; Gitelson, I.I.

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