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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Попова Л.Ю., Максимова Е.Е., Репета Т.В., Брильков А.В., Печуркин Н.С.
Заглавие : Экспрессия клонированных генов бактериальной люминесценции у микроорганизмов, выделенных из лабораторных микрокосмов : научное издание
Место публикации : Биотехнология. - 1994. - N 5. - С. 6-10. - ISSN 0234-2758
ГРНТИ : 62.37.99
Предметные рубрики: БИОЛЮМИНЕСЦЕНЦИЯ
ГЕНЫ
ЛЮМИНЕСЦЕНЦИИ
КЛОНИРОВАННЫЕ
ЭКСПРЕССИЯ ГЕНОВ
У МИКРООРГАНИЗМОВ
ИЗ МИКРОКОСМОВ
ЛАБОРАТОРНЫХ
ИЗУЧЕНИЕ
Аннотация: Изучали динамику развития люминесценции у исходного шт. E. coli Z 905, содержащего рекомбинантную плазмиду pPHL 7 (Ap{r}, Lux{+}), и некоторых типичных представителей бактериальной микрофлоры лаб. микрокосмов, захвативших клонированные гены биолюминесценции. Эти представители были определены как Arthrobacter spp., Micrococcus sp. и Cyanobacteria sp. Виды микроорганизмов, захвативших рекомбинантные ДНК, образуют колонии R- и S-типов. При этом колонии S-типа проявляли способность к более высокому уровню люминесценции по сравнению с колониями R-типа. Динамика развития люминесценции у ярких клонов Arthrobacter sp. не отличалась принципиально от динамики люминесценции шт. E. coli Z 905 (pPHL 7). У некоторых фенотипически темных колоний E. coli и некоторых природных микроорганизмов при культивировании в жидкой среде обнаружена слабая способность к люминесценции. Вариации экспрессии генов люминесценции могут быть связаны с различиями в метаболизме. Россия, Ин-т биофизики СО РАН, Красноярск, Академгородок, 660036. Библ. 30
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gaevsky N.A., Kolmakov V.I., Anishchenko O.V., Gorbaneva T.B.
Заглавие : Using DCMU-fluorescence method for the identification of dominant phytoplankton groups
Колич.характеристики :12 с
Место публикации : J. Appl. Phycol.: SPRINGER, 2005. - Vol. 17, Is. 6. - P483-494. - ISSN 0921-8971, DOI 10.1007/s10811-005-2903-x
Примечания : Cited References: 28
Предметные рубрики: IN-VIVO
CHLOROPHYLL FLUORESCENCE
FLUOROMETRIC METHOD
SITU
PHOTOSYNTHESIS
CYANOBACTERIA
ALGAE
EVOLUTION
CULTURES
LAKES
Ключевые слова (''Своб.индексиров.''): algae--chlorophyll--fluorescence--vizualization
Аннотация: For the identification of ecologically significant dominant groups of phytoplanktonic algae a polychromatic DCMU-induced fluorescence method is recommended. A special fluorometer equipped with a system of replaceable filters is used to differentiate three regions of the spectrum (410 +/- 20, 510 +/- 20 and 540 +/- 10 nm) that can excite the basic light-harvesting pigments. Total and differential (for every algal taxon studied) chlorophyll a calculated from the fluorescence signals is in good agreement with biomass estimates from direct cell counts for several different trophic types of aquatic systems. This is made possible by the vizualization of the ratios of fluorescence signal values in their own coordinates: first, to decide whether it is necessary to correct linear equations in order to eliminate negative solutions; second, to determine the possibility of nulling the negative solution if a point is situated close to a side of the triangle; and third, to reduce the number of linear algebraic equations to two if the points are situated along one of the triangle sides or to one if the points are gathered at the apex. The polychromatic DCMU-induced fluorescence method can be used for monitoring natural phytoplankton populations to detect changes in their taxonomic structure.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Degermendzhy A.G., Gulati R.D.
Заглавие : Understanding the mechanisms of blooming of phytoplankton in Lake Shira, a saline lake in Siberia (the Republic of Khakasia)
Место публикации : Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - С. 331-340. - ISSN 13862588 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon budget--cyanobacteria--heterotrophic bacteria--hydrogen sulphide--mathematical models of stratification--meromictic lakes--microbial loop--stratification--trophic scheme--algal bloom--ecosystem modeling--limiting factor--nutrient availability--phytoplankton--saline lake--trophic interaction--russian federation--algae--bacteria (microorganisms)--cyanobacteria--lyngbya--lyngbya contorta
Аннотация: The paper summarises the results of a three-year research study (European Union Grant: INTAS 97-0519) aimed at investigating the planktonic populations and trophic organization of the Lake Shira ecosystem - a saline lake in Khakasia, Siberia. The lake exhibits a stable summer-autumn stratification of the chemical-biological components. The mechanisms responsible for the 'blooming' of phytoplankton in the deeper layers were investigated in greater detail, using data from both field and laboratory experiments. The spectra of nutrition were examined to estimate the relationships between the specific growth rates of the hydrobionts and the influence of the limiting factors: light, nutrients. The observed heterotrophic capability of a metalimnetic phytoplankton population might help explain the development in the deeper waters of Lyngbya contorta. The scheme of trophic interactions was put up, based on the assessment of the carbon pools and carbon flows in the pelagic zone of the lake. A mathematical model of the vertical structure of the lake's plankton populations was constructed, using the ecosystem description and data of vertical turbulent diffusion. The role of light and nutrient limitations and grazing mortality in forming the vertical inhomogeneities, particularly in lowering the depth of the maximal cyanobacterial biomass, has been demonstrated. The theoretical curves for the stratification of chemical and biological parameters have been brought in conformity with the field observations, e.g. for the different patterns of the peaks, and for the biomass maxima of cyanobacteria, purple and green sulphur bacteria, oxygen, and hydrogen sulphide. The calculations revealed that for an adequate assessment of the parameters for the hydrogen sulphide zone it is necessary to introduce flows of allochthonous organic matter. Based on the form of the sulphur distribution curve, the allochthonous input of organic matter and the inflow of hydrogen sulphide from the bottom have been theoretically discriminated for the first time. It has also been ascertained that irrespective of the depth the allochthonous substances limiting bacterial growth, the bacteria are uniformly distributed over depth and can serve as an indicator of the presence of limitation (the effect of autostabilisation in space). Of indisputable interest to limnology are the specific methods developed for understanding the functioning of Lake Shira ecosystem. These include the autostabilisation of the limiting factors, the on-the-spot fluorescent method of determining the three classes of microalgae, the algal mixotrophy and the planktonic population interactions and feedbacks, and development of a more sensitive, bioluminescent method for mapping the nonhomogeneities. Owing to a balanced combination of classical approaches (field observations, in situ data on production-decomposition) and the more recent ones (satellite monitoring, biophysical methods of estimating interactions of populations, mathematical models based on the field and experimental data), many of the structural-function relationships in the ecosystem can now be explained, and the models can provide 'mutual control and mutual agreement' between the data collected using different approaches.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V.A., Esimbekova E.N., Gladyshev M.I., Khromichek E.B., Kuznetsov A.M., Ivanova E.A.
Заглавие : The use of bioluminescent biotests for study of natural and laboratory aquatic ecosystems
Место публикации : Chemosphere. - 2001. - Vol. 42, Is. 8. - С. 909-915. - ISSN 00456535 (ISSN) , DOI 10.1016/S0045-6535(00)00177-6
Ключевые слова (''Своб.индексиров.''): alcohol dehydrogenase--bacterial luciferase--bioluminescence--blooming--pollution--trypsin--water toxicity--alcohol dehydrogenase--benzoquinone--luciferase--trypsin--aquatic ecosystem--bioluminescence--water quality--article--bacterium culture--bioluminescence--blue green alga--ecosystem--pond--seasonal variation--water pollution--water quality--benzoquinones--biological assay--cyanobacteria--ecosystem--environmental monitoring--eutrophication--fmn reductase--indicators and reagents--luminescent measurements--nadh, nadph oxidoreductases--water pollutants--russian federation--algae--bacteria (microorganisms)--chlorophyta--cyanobacteria--uncultured cyanobacterium
Аннотация: A set of bioluminescent tests was developed to monitor water quality in natural and laboratory ecosystems. It consisted of four bioluminescent systems: luminous bacteria, coupled enzyme system NADH:FMN-oxidoreductase-luciferase and triplet enzyme systems with alcohol dehydrogenase and trypsin. The set of biotests was applied for a small forest pond (Siberia, Russia), laboratory microecosystems polluted with benzoquinone and a batch culture of blue-green algae. Thereby effects of natural water compared to those of models of heavy pollution and "bloom" of blue-greens on the bioluminescent tests were revealed. The set of biotests was not affected by a natural seasonal variability of water quality in the unpolluted pond, but responded to the heavy pollution and the "bloom" of blue-greens. The set of biotests could be recommended as the alarm test to control the acute toxicity of natural water bodies. В© 2001 Elsevier Science Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Makhutova O.N., Sushchik N.N., Kalacheva G.S.
Заглавие : The information value of fatty acid composition of triacylglycerols and polar lipids of seston in assaying the food spectrum of microzooplankton in the bugach reservoir.
Место публикации : Doklady biological sciences : proceedings of the Academy of Sciences of the USSR, Biological sciences sections / translated from Russian. - 2004. - Vol. 395. - С. 143-145. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): fatty acid--fresh water--lipid--triacylglycerol--article--bacterium--biomass--chemistry--cyanobacterium--diatom--ecosystem--metabolism--physiology--phytoplankton--russian federation--season--bacteria--biomass--cyanobacteria--diatoms--ecosystem--fatty acids--fresh water--lipids--phytoplankton--russia--seasons--triglycerides
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sid'ko A.F., Vasil'yev V.A.
Заглавие : Spectral brightness of inland waters and its correlation with phytoplankton content and hydrooptical characteristics
Место публикации : Hydrobiological Journal. - 1988. - Vol. 24, Is. 5. - С. 64-71. - ISSN 00188166 (ISSN)
Ключевые слова (''Своб.индексиров.''): cyanobacteria--diatom--light attenuation--pyrrhophyte--spectral brightness--ussr, dnieper--ussr, krasnoyarsk reservoir--algae--bacillariophyta--chlorophyta--cyanobacteria--dinophyceae--uncultured cyanobacterium
Аннотация: Spectral brightness coefficients are correlated with indices of vertical attenuation of horizontal irradiance, water transparency, phytoplankton chlorophyll content, and phytoplankton distribution. Data were obtained from Krasnoyarsk Reservoir and the Dnieper reservoirs. When diatoms and pyrrhophytes predominate, the maximum spectral brightness is in the vicinity of 580 nm, but when blue-green algae predominate the maxima are in the vicinity of 550 and 720 nm. -from Authors
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakov V.I., Gladyshev M.I., Kravchuk E.S., Chuprov S.M., Anishchenko O.V., Ivanova E.A., Trusova M.Yu.
Заглавие : Species-specific stimulation of cyanobacteria by silver carp Hypophthalmichthys molitrix (Val.)
Место публикации : Doklady Biological Sciences. - 2006. - Vol. 408, Is. 1. - С. 223-225. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496606030069
Ключевые слова (''Своб.индексиров.''): animal--article--carp--cyanobacterium--growth, development and aging--microbiology--species difference--animals--carps--cyanobacteria--species specificity--cyanobacteria--cyprinus carpio--hypophthalmichthys molitrix--hypophthalmichthys nobilis
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kravchuk E.S., Ivanova E.A., Gladyshev M.I.
Заглавие : Spatial distribution of resting stages (akinetes) of the cyanobacteria Anabaena flos-aquae in sediments and its influence on pelagic populations
Место публикации : Marine and Freshwater Research. - 2011. - Vol. 62, Is. 5. - С. 450-461. - ISSN 13231650 (ISSN) , DOI 10.1071/MF10256
Ключевые слова (''Своб.индексиров.''): cyanobacterial blooms--harmful algae--reservoir--resting stages--sediment management--water quality--abundance--bioaccumulation--biomass--cyanobacterium--environmental factor--experimental study--green alga--inoculation--littoral environment--macrophyte--pelagic environment--phytoplankton--population structure--qualitative analysis--recruitment (population dynamics)--reservoir--seasonal variation--sediment analysis--silt--spatial distribution--vertical distribution--water quality--siberia--algae--anabaena flos-aquae--cyanobacteria
Аннотация: Recruitment of pelagic populations of cyanobacteria from littoral bottom sediments is assumed to be one of the key factors for the development of harmful blooms. Therefore, it is necessary to obtain more information on the possible factors influencing the spatial distribution of the resting stages in littoral bottom sediments. To test the hypothesis that akinete abundances in littoral bottom sediments are associated with silt and the presence of higher water plants, we sampled spatial and vertical distribution and seasonal dynamics of akinetes of cyanobacterium Anabaena flos-aquae in a small Siberian reservoir. We found that akinetes accumulate in silt at stations located in macrophyte beds. Two experiments were carried out to test if there is relationship between size of inoculum and size of the pelagic population of A. flos-aquae. The proportion of A. flos-aquae in total phytoplankton biomass depended on the initial abundance of akinetes in bottom sediments. The model based on laboratory experiments gave good qualitative description of the field data. These findings can be useful to predict the location of the main akinete accumulation zones in a water body and plan the removal of sediment to prevent the cyanobacterial blooms. В© CSIRO 2011.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakova O.V., Gladyshev M.I., Rozanov A.S., Peltek S.E., Trusova M.Y.
Заглавие : Spatial biodiversity of bacteria along the largest Arctic river determined by next-generation sequencing
Место публикации : FEMS Microbiol. Ecol. - 2014. - Vol. 89, Is. 2. - С. 442-450. - ISSN 15746941 (ISSN) , DOI 10.1111/1574-6941.12355
Ключевые слова (''Своб.индексиров.''): 16s rrna gene--bacterial community--diversity--yenisei river--actinobacteria--bacteria (microorganisms)--cyanobacteria--proteobacteria
Аннотация: The biodiversity of bacterial communities along the Yenisei River at section c. 1800 km was studied using next-generation sequencing of 16S rRNA genes and common biodiversity indices. Overall, 3022 unique operational taxonomic units were identified. Actinobacteria and Proteobacteria were the dominant phyla at all sampling sites. The highest alpha-diversity values were found in the middle section of the studied river. The beta-diversity of bacterial assemblages in the river was related to the surrounding landscape (biome): three distinctly different bacterial assemblages occurred in sections of the river, situated in mountain taiga, plain taiga and in a region of permafrost, covered by forest-tundra and tundra. Tributaries arising from these different landscapes likely contributed substantially to the variations of Yenisei bacterial communities. In contrast to a prediction of the river continuum concept, the proportion of photoautotrophic Cyanobacteria in bacterial assemblages did not increase downstream, but peaked at the middle section. © 2014 Federation of European Microbiological Societies.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakova O.V., Gladyshev M.I., Rozanov A.S., Peltek S.E., Trusova M.Y.
Заглавие : Spatial biodiversity of bacteria along the largest Arctic river determined by next-generation sequencing
Колич.характеристики :9 с
Коллективы : Attracting Leading Scientists to Russian Educational Institutions Program of the Russian Federation [11.G34.31.0014]; Siberian Federal University
Место публикации : FEMS Microbiol. Ecol.: WILEY-BLACKWELL, 2014. - Vol. 89, Is. 2. - С. 442-450. - ISSN 0168-6496, DOI 10.1111/1574-6941.12355. - ISSN 1574-6941
Примечания : Cited References: 36. - This work was supported by the Attracting Leading Scientists to Russian Educational Institutions Program of the Russian Federation, agreement 11.G34.31.0014, and by the project G-1 of Siberian Federal University, carried out according to Federal tasks of the Ministry of Education and Science of Russian Federation.
Предметные рубрики: DISSOLVED ORGANIC-MATTER
INLAND WATERS
CARBON
BACTERIOPLANKTON
COMMUNITY
GREENGENES
ECOSYSTEM
RESERVOIR
PATTERNS
PRIMERS
Ключевые слова (''Своб.индексиров.''): bacterial community--diversity--16s rrna gene--yenisei river
Аннотация: The biodiversity of bacterial communities along the Yenisei River at section c. 1800 km was studied using next-generation sequencing of 16S rRNA genes and common biodiversity indices. Overall, 3022 unique operational taxonomic units were identified. Actinobacteria and Proteobacteria were the dominant phyla at all sampling sites. The highest alpha-diversity values were found in the middle section of the studied river. The beta-diversity of bacterial assemblages in the river was related to the surrounding landscape (biome): three distinctly different bacterial assemblages occurred in sections of the river, situated in mountain taiga, plain taiga and in a region of permafrost, covered by forest-tundra and tundra. Tributaries arising from these different landscapes likely contributed substantially to the variations of Yenisei bacterial communities. In contrast to a prediction of the river continuum concept, the proportion of photoautotrophic Cyanobacteria in bacterial assemblages did not increase downstream, but peaked at the middle section.
WOS
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Kalacheva G.S., Gladyshev M.I.
Заглавие : Secretion of free fatty acids by prokaryotic and eukaryotic algae at optimal, supraoptimal, and suboptimal growth temperatures
Место публикации : Mikrobiologiya. - 2001. - Vol. 70, Is. 5. - С. 629-635. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): free fatty acids--microalgae--secretion--fatty acid--article--chemistry--chlorella--cyanobacterium--cytology--extracellular space--growth, development and aging--intracellular fluid--temperature--chlorella--cyanobacteria--english abstract--extracellular space--fatty acids, nonesterified--intracellular fluid--temperature
Аннотация: Abstract-The paper describes the composition of extracellular free fatty acids (FFAs) and intracellular fatty acids (FAs) in the enrichment cultures of the prokaryotic alga Spirulina platensis and the eukaryotic alga Chlorella vulgaris grown at optimal, supraoptimal, and suboptimal growth temperatures. With increasing growth temperature, the degree of unsaturation of the intracellular FAs of both algae decreased, while that of the extracellular FFAs of 5. platensis increased. The composition of the extracellular FFAs of C. vulgaris practically did not depend on the growth temperature.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Anishchenko O.V., Gladyshev M.I., Kravchuk E.S., Ivanova E.A., Gribovskaya I.V., Sushchik N.N.
Заглавие : Seasonal variations of metal concentrations in periphyton and taxonomic composition of the algal community at a Yenisei River littoral site
Место публикации : Central European Journal of Biology. - 2010. - Vol. 5, Is. 1. - С. 125-134. - ISSN 1895104X (ISSN) , DOI 10.2478/s11535-009-0060-y
Ключевые слова (''Своб.индексиров.''): heavy metals--periphyton--seasonal dynamics--algae--chlorophyta--cyanobacteria--ulothrix zonata
Аннотация: The concentrations of metals K, Na, Ca, Mg, Fe, Mn, Zn, Cu, Ni, Pb, Co and Cr, in the water and periphyton (epilithic algal communities) were studied at a site in the middle stream of the Yenisei River (Siberia, Russia) during three years using monthly sampling frequencies. Despite considerable seasonal variations in aquatic concentrations of some metals, there was no correlation between metal contents in the water and in periphyton. Seasonal concentration variations of some metals in periphyton were related to the species (taxonomic) composition of periphytic microalgae and cyanobacteria. Enhanced levels of Ni and Co in periphyton in late autumn, winter, and early spring were likely caused by the predominance of cyanobacteria in the periphytic community, and annual maximum levels of K in periphyton in late spring and early summer were attributed to the domination of Chlorophyta, primarily Ulothrix zonata. В© Versita Warsaw and Springer-Verlag Berlin Heidelberg.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kalachova G.S., Kolmakova A.A., Gladyshev M.I., Kravchuk E.S., Ivanova E.A.
Заглавие : Seasonal dynamics of amino acids in two small Siberian reservoirs dominated by prokaryotic and eukaryotic phytoplankton
Колич.характеристики :13 с
Место публикации : Aquat. Ecol.: KLUWER ACADEMIC PUBL, 2004. - Vol. 38, Is. 1. - P3-15. - ISSN 1386-2588, DOI 10.1023/B:AECO.0000021044.55658.71
Примечания : Cited References: 37
Предметные рубрики: DISSOLVED ORGANIC-MATTER
PARTICULATE FATTY-ACIDS
FRESH-WATER ECOSYSTEMS
BACTERIAL PRODUCTION
CHEMICAL-COMPOSITION
RATES
MICROALGAE
PROTEINS
RELEASE
DAPHNIA
Ключевые слова (''Своб.индексиров.''): amino acids--phytoplankton--cyanobacteria--blooms
Аннотация: The comparison of the dynamics of phytoplankton biomass and total amino acid composition was made for two water bodies: in one the phytoplankton were dominated by prokaryotes (i.e., there was a bloom of cyanobacteria) and by eukaryotic microalgae in the other. The dynamics of phytoplankton biomass and of total amino acid composition of water were investigated during the vegetation season. It was found that the only factor that significantly changed the percentages of amino acids in water was the bloom of cyanobacteria in the "blooming" water body. During the bloom of cyanobacteria, the absolute and relative content of the Leu-Glu group increased, while the contents of other acids generally dropped. Before and after the bloom, no significant variations in the total amino acid composition were recorded. In the reservoir where eukaryotic microalgae dominated, no significant variations in amino acid composition were recorded during the season.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kalachova G.S., Kolmakova A.A., Gladyshev M.I., Kravchuk E.S., Ivanova E.A.
Заглавие : Seasonal dynamics of amino acids in two small Siberian reservoirs dominated by prokaryotic and eukaryotic phytoplankton
Место публикации : Aquatic Ecology. - 2004. - Vol. 38, Is. 1. - С. 3-15. - ISSN 13862588 (ISSN) , DOI 10.1023/B:AECO.0000021044.55658.71
Ключевые слова (''Своб.индексиров.''): amino acids--blooms--cyanobacteria--phytoplankton--amino acid--biomass--phytoplankton--reservoir--seasonal variation--eurasia--siberia--cyanobacteria--eukaryota--prokaryota
Аннотация: The comparison of the dynamics of phytoplankton biomass and total amino acid composition was made for two water bodies: in one the phytoplankton were dominated by prokaryotes (i.e., there was a bloom of cyanobacteria) and by eukaryotic microalgae in the other. The dynamics of phytoplankton biomass and of total amino acid composition of water were investigated during the vegetation season. It was found that the only factor that significantly changed the percentages of amino acids in water was the bloom of cyanobacteria in the "blooming" water body. During the bloom of cyanobacteria, the absolute and relative content of the Leu-Glu group increased, while the contents of other acids generally dropped. Before and after the bloom, no significant variations in the total amino acid composition were recorded. In the reservoir where eukaryotic microalgae dominated, no significant variations in amino acid composition were recorded during the season.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kravchuk E.S., Ivanova E.A., Gladyshev M.I.
Заглавие : Seasonal dynamics of akinetes of Anabaena flos-aquae in bottom sediments and water column of small Siberian reservoir
Место публикации : Aquatic Ecology. - 2006. - Vol. 40, Is. 3. - С. 325-336. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-006-9031-9
Ключевые слова (''Своб.индексиров.''): akinetes--cyanobacteria--cyanobacterial blooms--resting stages--abundance--alga--intertidal environment--open water--population dynamics--reservoir--sediment--water column--eurasia--siberia--anabaena--anabaena flos-aquae--cyanobacteria
Аннотация: Seasonal dynamics of Anabaena flos-aquae (Lyngb.) Breb., including vegetative cells, akinetes and akinete envelopes, in bottom sediments and water column at both littoral and deeper central stations of a small Siberian reservoir was studied. Two types of akinetes were observed: in the first half of summer Anabaena formed akinetes, which served for vegetative reproduction and germinated in water column soon after differentiation, while in the second half of summer the akinetes produced served as a resting stages, which were deposited to bottom sediments. Canonical correlation analyses revealed that decrease of water temperature was the main environmental factor that stimulated the akinete formation. In contrast to the general opinion, concentration of inorganic phosphorus slightly, but positively influenced the akinete formation. Thus, akinetes formed in response to the temperature decrease, needs a certain level of this nutrient. At littoral and open-water stations abundance and seasonal dynamics of akinetes in water column and their sinking pattern were very similar. However, seasonal dynamics of abundance of akinetes in sediments in these two reservoir locations differed: whereas the abundance of akinetes in open water increased permanently during the summer, that in the littoral decreased soon after their sedimentation. The cause for decrease in abundance of akinetes in bottom sediments in winter is unknown. В© Springer 2006.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Ivanova E.A., Kravchuk E.S.
Заглавие : Seasonal distribution and fatty acid composition of littoral microalgae in the Yenisei River
Место публикации : Journal of Applied Phycology. - 2010. - Vol. 22, Is. 1. - С. 11-24. - ISSN 09218971 (ISSN) , DOI 10.1007/s10811-009-9418-9
Ключевые слова (''Своб.индексиров.''): fatty acids--riverine microalgae--taxa composition--temperature adaptations--algae--bacillariophyta--chlorophyta--cyanobacteria
Аннотация: We studied fatty acid (FA) composition of littoral microalgae in the fast-flowing oligotrophic river, the Yenisei, Siberia, monthly for 3 years. Seasonal dynamics of species composition had similar patterns in all the studied years. In springs, a pronounced dominance of filamentous green algae occurred, in summer and autumn diatoms were abundant, and in late autumn and winter epilithic biofilms consisted primarily of cyanobacteria and detritus. In general, FA composition of the algal periphytic community was dominated by 16:0, 16:1?7, 20:5?3, 14:0, and 18:3?3 throughout the studied period. Several groups of FAs, which had peculiar seasonal dynamics, were differentiated by statistical analysis based on a method of correlation graphs. The seasonal changes in FA composition could be partly explained by the seasonal succession of species composition of the community. Besides, we found that populations of both diatom and green algae grown in summer at a higher water temperature were lower in polyunsaturated fatty acids than those in spring, at a lower temperature. Hence, we suppose that the regular seasonal dynamics of FA composition of the studied littoral microalgae was driven both by changes in species composition and by temperature adaptations of the algal populations. The highest content of essential polyunsaturated FAs, eicosapentaenoic and docosahexaenoic acids, in the spring "psychrophilic" populations of diatoms could make them of the higher nutritive value for zoobenthic primary consumers. В© Springer Science+Business Media B.V. 2009.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Sushchik N.N., Kolmakova A.A., Kalachova G.S., Kravchuk E.S., Ivanova E.A., Makhutova O.N.
Заглавие : Seasonal correlations of elemental and ?3 PUFA composition of seston and dominant phytoplankton species in a eutrophic Siberian Reservoir
Место публикации : Aquatic Ecology. - 2007. - Vol. 41, Is. 1. - С. 9-23. - ISSN 13862588 (ISSN) , DOI 10.1007/s10452-006-9040-8
Ключевые слова (''Своб.индексиров.''): elemental stoichiometry--phytoplankton--polyunsaturated fatty acids--seston--carbon--dominance--eutrophic environment--fatty acid--nitrogen--nutritional requirement--nutritive value--phosphorus--phytoplankton--seasonal variation--seston--zooplankton--eurasia--siberia--anabaena--bacillariophyta--cyanobacteria--gomphosphaeria--peridinium--planktothrix--stephanodiscus
Аннотация: The elemental and fatty acid composition of seston was studied for 3 years, from May to October, in a small Reservoir. Under comparatively low C:P ratio, multivariate canonical analysis revealed no straightforward simple correlations between phosphorus and single ?3 PUFA species, but complex significant interaction between elemental composition (stoichiometry) of seston and total sestonic ?3 PUFA as a whole. Since sestonic C, P and N were found to originate mostly from phytoplankton, the contents of particulate elements and PUFA were attributed to single species in periods of their pronounced dominance. Phytoplankton species of genera of Stephanodiscus, Peridinium, Gomphosphaeria, Planktothrix and Anabaena in periods of their pronounced dominance had relatively constant species-specific elemental and PUFA composition. Phytoplankton species significantly differed in their elemental and PUFA composition, as well as in ratios of C:N, N:P, PUFA:P and partly C:P that indicate food quality for zooplankton. Hence, there were no phytoplankton species of clearly high or low nutritional value. All of phytoplankters, or at least detritus, that originated from them, may meet specific elemental and biochemical requirements of specific groups of zooplankton. Dividing phytoplankton on basis of their elemental and biochemical composition, i.e., nutrition quality, into large taxa (cyanobacteria, diatoms, etc.) appeared to be too coarse for assessing nutritional value for zooplankton. В© Springer Science+Business Media B.V. 2006.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Anishchenko O.V., Komakov V.I., Gladyshev M.I.
Заглавие : Research of the meteorological factors influence on the fluorescence measurements of subsurface phytoplankton in blooming reservoir
Место публикации : Doklady Akademii Nauk. - 2004. - Vol. 397, Is. 1. - С. 124-127. - ISSN 08695652 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacteria--fluorescence--meteorological problems--precipitation (meteorology)--reservoirs (water)--solar radiation--wind effects--meteorological effects--algae
Аннотация: The reaction of algae and cyano-bacteria photosynthetic apparatus on atmospheric sediments, incident solar radiation and wind velocity was estimated by measuring chlorophyll fluorescence using three-beam fluorescent diagnostics. As shown, wind in the spring positively affects the chlorophyll concentration but causes negative effect on phytoplankton photosynthetic activity in other seasons. Under conditions of high-intensity solar irradiation confervoid and colonial cyanobacteria, possessing gas vacuoles, have an advantage over plankton eukaryotic algae. Other response differences weren't observed.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tolomeyev A.P.
Заглавие : Phytoplankton diet of Arctodiaptomus salinus (Copepoda, Calanoida) in Lake Shira (Khakasia)
Место публикации : Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - С. 229-234. - ISSN 13862588 (ISSN) , DOI 10.1023/A:1015618104520
Ключевые слова (''Своб.индексиров.''): arctodiaptomus salinus--food selectivity--phytoplankton--diet--food selection--phytoplankton--saline lake--zooplankton--russian federation--algae--animalia--arctodiaptomus--calanoida--copepoda--cyanobacteria--eukaryota--invertebrata--lyngbya--lyngbya contorta--microcystis--microcystis sp.
Аннотация: A flow-through method was employed to study the algal part of the diet spectrum of the calanoid Arctodiaptomus salinus in the surface layer of the lake and in the phytoplankton biomass maximum zone (10 m deep). Daily consumption rates of this calanoid differed markedly in these layers, being 1 and 11 ?g ind -1 d-1 respectively. The cyanobacterial taxa, Lyngbya contorta and Microcystis sp., accounted for more than a half of the ration. Nevertheless, the negative Ivlev electivity coefficient indicated that Arctodiaptomus prefers none of these two taxa. The low measured uptakes suggest that the energy expenditure is compensated by other resources, presumably microzooplankton and detritus.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Trusova M.Yu., Gladyshev M.I.
Заглавие : Phylogenetic diversity of winter bacterioplankton of eutrophic Siberian reservoirs as revealed by 16S rRNA gene sequences
Место публикации : Microbial Ecology. - 2002. - Vol. 44, Is. 3. - С. 252-259. - ISSN 00953628 (ISSN) , DOI 10.1007/s00248-002-2020-1
Ключевые слова (''Своб.индексиров.''): bacterioplankton--community composition--community dynamics--genetic variation--phylogenetics--reservoir--winter--russian federation--actinobacteria--bacteria (microorganisms)--bacteroides--betaproteobacteria--cyanobacteria--cytophaga--flavobacterium--prokaryota--proteobacteria--bacterial dna--fresh water--rna 16s--animal--article--bacterium--chemistry--dna sequence--genetic variability--genetics--microbiology--molecular genetics--phylogeny--plankton--russian federation--season--animals--bacteria--dna, bacterial--fresh water--molecular sequence data--phylogeny--plankton--rna, ribosomal, 16s--seasons--sequence analysis, dna--siberia--variation (genetics)
Аннотация: Using 16S rRNA gene sequence analyses we investigated the bacterial diversity of winter bacterioplankton of two eutrophic Siberian reservoirs. These reservoirs show similarity in phytoplankton community composition in spring and autumn but tend to differ in summer in exhibiting cyanobacterial bloom. Forty-eight unique partial 16S RNA gene sequences retrieved from two libraries were mostly affiliated with the class Actinobacteria, ? subdivision of the class Proteobacteria, and the phylum Cytophaga-Flavobacterium-Bacteroides. The clone library of the pond exhibiting summer cyanobacterial bloom showed more diversity in sequence composition. A significant number of bacterial 16S rRNA gene clones were closely related to freshwater bacteria previously found in different aquatic ecosystems. This finding confirms the assumption that some bacterial clades are globally distributed.
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