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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Neubauer, Darshan, Kolmakova, Olesya, Woodhouse, Jason, Taube, Robert, Mangelsdorf, Kai, Gladyshev, Michail, Premke, Katrin, Grossart, Hans-Peter
Заглавие : Zooplankton carcasses stimulate microbial turnover of allochthonous particulate organic matter
Колич.характеристики :16 с
Коллективы : Projekt DEAL
Место публикации : ISME J.: SPRINGERNATURE, 2021. - Article in press. - ISSN 1751-7362, DOI 10.1038/s41396-020-00883-w. - ISSN 1751-7370(eISSN)
Примечания : Cited References:83. - Open Access funding enabled and organized by Projekt DEAL.
Предметные рубрики: FRESH-WATER
SEASONAL-CHANGES
CARBON
LAKE
DECOMPOSITION
DEGRADATION
Аннотация: Carbon turnover in aquatic environments is dependent on biochemical properties of organic matter (OM) and its degradability by the surrounding microbial community. Non-additive interactive effects represent a mechanism where the degradation of biochemically persistent OM is stimulated by the provision of bioavailable OM to the degrading microbial community. Whilst this is well established in terrestrial systems, whether it occurs in aquatic ecosystems remains subject to debate. We hypothesised that OM from zooplankton carcasses can stimulate the degradation of biochemically persistent leaf material, and that this effect is influenced by the daphnia:leaf OM ratio and the complexity of the degrading microbial community. Fresh Daphnia magna carcasses and C-13-labelled maize leaves (Zea mays) were incubated at different ratios (1:1, 1:3 and 1:5) alongside either a complex microbial community (50 mu m) or solely bacteria (0.8 mu m). C-13 stable-isotope measurements of CO2 analyses were combined with phospholipid fatty acids (PLFA) analysis and DNA sequencing to link metabolic activities, biomass and taxonomic composition of the microbial community. Our experiments indicated a significantly higher respiration of leaf-derived C when daphnia-derived OM was most abundant (i.e. daphnia:leaf OM ratio of 1:1). This process was stronger in a complex microbial community, including eukaryotic microorganisms, than a solely bacterial community. We concluded that non-additive interactive effects were a function of increased C-N chemodiversity and microbial complexity, with the highest net respiration to be expected when chemodiversity is high and the degrading community complex. This study indicates that identifying the interactions and processes of OM degradation is one important key for a deeper understanding of aquatic and thus global carbon cycle.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Burakova, Ludmila P., Lyakhovich, Maria S., Mineev, Konstantin S., Petushkov, Valentin N., Zagitova, Renata, I, Tsarkova, Aleksandra S., Kovalchuk, Sergey, I, Yampolsky, Ilia, V, Vysotski, Eugene S., Kaskova, Zinaida M.
Заглавие : Unexpected Coelenterazine Degradation Products of Beroe abyssicola Photoprotein Photoinactivation
Колич.характеристики :4 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [20-04-00085]; Russian Foundation for Basic Research, Krasnoyarsk Territory [20-44-242003]; Krasnoyarsk Regional Fund of Science in part of purification and spectral characterization of native compounds; Russian Science FoundationRussian Science Foundation (RSF) [17-1401169p]; Russian FederationRussian Federation [LS-2605.2020.4]
Место публикации : Org. Lett.: AMER CHEMICAL SOC, 2021. - Vol. 23, Is. 17. - С. 6846-6849. - ISSN 1523-7060, DOI 10.1021/acs.orglett.1c02410. - ISSN 1523-7052(eISSN)
Примечания : Cited References:20. - This work was supported by grant 20-04-00085 of the Russian Foundation for Basic Research, grant 20-44-242003 of the Russian Foundation for Basic Research, Krasnoyarsk Territory, and Krasnoyarsk Regional Fund of Science in part of purification and spectral characterization of native compounds, grant 17-1401169p of the Russian Science Foundation, and the President of Russian Federation grant for Leading Scientific Schools LS-2605.2020.4 in part of structural elucidation of native products and organic synthesis. We thank Konstantin Antonov (IBCh RAS) and Igor Ivanov (IBCh RAS) for the registration of HRMS spectra.
Предметные рубрики: CRYSTAL-STRUCTURE
BIOLUMINESCENCE
OBELIN
RESIDUES
BINDING
Аннотация: Ca2+-regulated photoproteins of ctenophores lose bioluminescence activity when exposed to visible light. Little is known about the chemical nature of chromophore photo-inactivation. Using a total synthesis strategy, we have established the structures of two unusual coelenterazine products, isolated from recombinant berovin of the ctenophore Beroe abyssicola, which are Z/E isomers. We propose that during light irradiation, these derivatives are formed from 2-hydroperoxycoelenterazine via the intermediate 8a-peroxide by a mechanism reminiscent of that previously described for the auto-oxidation of green-fluorescent-protein-like chromophores.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E., Menzyanova N., Zhila N., Prudnikova S., Volova T., Thomas S.
Заглавие : Toxic effects of the fungicide tebuconazole on the root system of fusarium-infected wheat plants
Место публикации : Plant Physiol. Biochem.: Elsevier Masson SAS, 2018. - Vol. 132. - С. 400-407. - ISSN 09819428 (ISSN) , DOI 10.1016/j.plaphy.2018.09.025
Ключевые слова (''Своб.индексиров.''): border cells--carbonylated proteins--free proline--fusarium--malondialdehyde--tebuconazole
Аннотация: The study investigates toxic effects of the fungicide tebuconazole (TEB) on Fusarium-infected wheat (Triticum aestivum) plants based on the morphological characteristics of root apices and changes in the integrated parameters of redox homeostasis, including the contents of free proline and products of peroxidation of proteins (carbonylated proteins, CP) and lipids (malondialdehyde, MDA) in roots. In two-day-old wheat sprouts infected by Fusarium graminearum, the levels of proline, CP, and border cells of root apices are higher than in roots of uninfected sprouts by a factor of 1.4, 8.0, and 3, respectively. The triazole fungicide tebuconazole (TEB) at the concentrations of 0.01, 0.10, and 1.00 ?g ml?1 of medium causes a dose-dependent decrease in the number of border cells. The study of the effects of TEB and fusarium infection on wheat plants in a 30-day experiment shows that the effect of the fungicide TEB on redox homeostasis in wheat roots varies depending on the plant growth stage and is significantly different in ecosystems with soil and plants infected by Fusarium phytopathogens. The study of the morphology of root apices shows that the toxic effects of TEB and fusarium infection are manifested in the destructive changes in root apices and the degradation of the root tip mantle. © 2018 Elsevier Masson SAS
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogilnaya O.A., Efremov S.N.
Заглавие : Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures
Колич.характеристики :10 с
Место публикации : J. Mater. Sci.-Mater. Med.: KLUWER ACADEMIC PUBL, 2004. - Vol. 15, Is. 6. - P719-728. - ISSN 0957-4530, DOI 10.1023/B:JMSM.0000030215.49991.0d
Примечания : Cited References: 34
Предметные рубрики: DEGRADATION
POLYESTERS
POLYMERS
FIBERS
PHB
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types-polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV)-featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 mum thick, which became as thin as 40-60 mum after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner. (C) 2004 Kluwer Academic Publishers.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Thomas S., Shumilova A. A., Kiselev E. G., Baranovsky S. V., Vasiliev A. D., Nemtsev I. V., Kuzmin A. P., Sukovatyi A. G., Avinash R. P., Volova T. G.
Заглавие : Thermal, mechanical and biodegradation studies of biofiller based poly-3-hydroxybutyrate biocomposites
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2019. - Article in press. - ISSN 01418130 (ISSN) , DOI 10.1016/j.ijbiomac.2019.11.112
Аннотация: Biodegradable poly-3-hydroxybutyrate [P(3HB)] and natural fillers - clay, peat, and birch wood flour – were used to prepare powdered composites to form pellets and granules. Pellets were produced by cold pressing of polymer and filler powder whereas granules were produced from the powders wetted with ethanol. Characterization techniques like IR spectroscopy, differential scanning calorimetry, X-ray analysis, mechanical analysis and electron microscopy were employed to study the properties of the initial P(3HB) and fillers and the composites. Analysis of the IR spectra of the composites showed the absence of chemical bonds between the components, i.e. the composites were physical mixtures. Young's moduli of the pellets prepared from initial materials varied considerably, and the highest value was obtained for P(3HB) pellets (350 MPa). Studies of biodegradation of composite pellets and granules in the soil for 35 days showed that the residual mass of the pellets had decreased to 68% for P(3HB); 56.4% for P(3HB)/peat; 67% for P(3HB)/wood flour, and 64% for P(3HB)/clay; granules exhibited a similar mass loss, residual mass of the granules of P(3HB) was 68.4%, P(3HB)/peat 46.4%; P(3HB)/wood flour 77%, and P(3HB)/clay 74%. This shows the significance of the material as an eco-friendly composite without sacrificing its mechanical properties. © 2018
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Thomas, Sabu, Shumilova A. A., Kiselev E. G., Baranovsky, S., V, Vasiliev A. D., Nemtsev, I., V, Kuzmin, Andrei Petrovich, Sukovatyi A. G., Avinash, R. Pai, Volova T. G.
Заглавие : Thermal, mechanical and biodegradation studies of biofiller based poly-3-hydroxybutyrate biocomposites
Колич.характеристики :12 с
Коллективы : Government of the Russian Federation [220]; Project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]
Место публикации : Int. J. Biol. Macromol.: ELSEVIER, 2020. - Vol. 155. - С. 1373-1384. - ISSN 0141-8130, DOI 10.1016/j.ijbiomac.2019.11.112. - ISSN 1879-0003(eISSN)
Примечания : Cited References:38. - This studywas financially supported by Project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328) in accordance with Resolution No 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning".; The surface of the samples was investigated using a scanning electron microscope Hitachi TM-3000 in the Joint Instrument Use Center at the Krasnoyarsk Scientific Center of Siberian Branch of Russian Academy of Sciences.
Предметные рубрики: FORMULATIONS
POLYHYDROXYALKANOATES
POLYHYDROXYBUTYRATE
SOIL
Аннотация: Biodegradable poly-3-hydroxybutyrate [P(3HB)] and natural fillers - clay, peat, and birch wood flour - were used to prepare powdered composites to form pellets and granules. Pellets were produced by cold pressing of polymer and filler powder whereas granules were produced from the powders wetted with ethanol. Characterization techniques like IR spectroscopy, differential scanning calorimetry, X-ray analysis, mechanical analysis and electron microscopy were employed to study the properties of the initial P(3HB) and fillers and the composites. Analysis of the IR spectra of the composites showed the absence of chemical bonds between the components, i.e. the composites were physical mixtures. Young's moduli of the pellets prepared from initial materials varied considerably, and the highest value was obtained for P(3HB) pellets (350 MPa). Studies of biodegradation of composite pellets and granules in the soil for 35 days showed that the residual mass of the pellets had decreased to 68% for P (3HB); 56.4% for P(3HB)/peat; 67% for P(3HB)/wood flour, and 64% for P(3HB)/clay; granules exhibited a similar mass loss, residual mass of the granules of P(3HB) was 68.4%, P(3HB)/peat 46.4%; P(3HB)/wood flour 77%, and P (3HB)/clay 74%. This shows the significance of the material as an eco-friendly composite without sacrificing its mechanical properties. (C) 2019 Published by Elsevier B.V.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Zhila N.O., Shishatskaya E.I., Mironov P.V., Vasil'Ev A.D., Sukovatyi A.G., Sinskey A.J.
Заглавие : The physicochemical properties of polyhydroxyalkanoates with different chemical structures
Место публикации : Polymer Science - Series A. - 2013. - Vol. 55, Is. 7. - С. 427-437. - ISSN 0965545X (ISSN) , DOI 10.1134/S0965545X13070080
Ключевые слова (''Своб.индексиров.''): amorphous and crystalline phasis--degrees of crystallinity--physicochemical property--poly(3-hydroxybutirate)--polyhydroxyalkanoate copolymer--temperature characteristic--thermal degradation temperatures--thermoplastic materials--copolymers--monomers--thermoplastic elastomers--structure (composition)
Аннотация: A set of polyhydroxyalkanoates are synthesized, and a comparative study of their physicochemical properties is performed. The molecular masses and polydispersities of polyhydroxyalkanoates are found to be independent of their chemical structures. It is shown that the temperature characteristics and degrees of crystallinity of polyhydroxyalkanoates are affected by the chemical compositions of the monomers and their quantitative contents in the polymers. The incorporation of 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate units into the chain of poly(3-hydroxybutyrate) decreases its melting point and thermal degradation temperature relative to these parameters of a homogeneous poly(3-hydroxybutyrate) sample (175 В± 5 C and 275 В± 5 C, respectively). The higher the content of the second monomer units in the poly(3-hydroxybutirate) chain, the greater the changes. The degrees of crystallinity of polyhydroxyalkanoate copolymers are generally lower than that of poly(3-hydroxybutyrate) (75 В± 5%). The effect on the ratio of the amorphous and crystalline phases of the copolymer samples becomes more pronounced in the series 3-hydroxy-valerate-3-hydroxyhexanoate-4- hydroxybutyrate. The prepared samples exhibit different properties ranging from rigid thermoplastic materials to engineering elastomers. В© 2013 Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova, Svetlana, Streltsova, Nadezhda, Volova, Tatiana
Заглавие : The effect of the pesticide delivery method on the microbial community of field soil
Колич.характеристики :17 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation, project "Agropreparations of the new generation: a strategy of construction and realization" [074-02-2018-328]
Место публикации : Environ. Sci. Pollut. Res.: SPRINGER HEIDELBERG, 2020. - Article in press. - ISSN 0944-1344, DOI 10.1007/s11356-020-11228-7. - ISSN 1614-7499(eISSN)
Примечания : Cited References:119. - This study was financially supported by the Ministry of Science and Higher Education of the Russian Federation, project "Agropreparations of the new generation: a strategy of construction and realization" (Agreement No 074-02-2018-328).
Предметные рубрики: CONTROLLED-RELEASE
2,4-DICHLOROPHENOXYACETIC ACID
DEGRADATION
Аннотация: The study deals with the effects of herbicides (metribuzin, tribenuron-methyl, fenoxaprop-P-ethyl) and fungicides (tebuconazole, epoxiconazole, azoxystrobin) applied to soil as free pesticides or as slow release formulations embedded in a biodegradable composite matrix on the structure of the soil microbial community. The matrix consisted of a natural biopolymer poly-3-hydroxybutyrate [P(3HB)] and a filler-one of the natural materials (peat, clay, and wood flour). The soil microbial community was characterized, including the major eco-trophic groups of bacteria, dominant taxa of bacteria and fungi, and primary P(3HB)-degrading microorganisms, such asPseudomonas,Bacillus,Pseudarthrobacter,Streptomyces,Penicillium, andTalaromyces. The addition of free pesticides adversely affected the abundance of soil microorganisms; the decrease varied from 1.4 to 56.0 times for different types of pesticides. The slow release pesticide formulations, in contrast to the free pesticides, exerted a much weaker effect on soil microorganisms, no significant inhibition in the abundance of saprotrophic bacteria was observed, partly due to the positive effects of the composite matrix (polymer/natural material), which was a supplementary substrate for microorganisms. The slow release fungicide formulations, like the free fungicides, reduced the total abundance of fungi and inhibited the development of the phytopathogensFusariumandAlternaria. Thus, slow release formulations of pesticides preserve the bioremediation potential of soil microorganisms, which are the main factor of removing xenobiotics from the biosphere.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kiselev E. G., Vasiliev A. D., Volova T. G.
Заглавие : Synthesis and characterization of multicomponent PHAs
Место публикации : J. Sib. Fed. Univ. - Biol.: Siberian Federal University, 2021. - Vol. 14, Is. 1. - С. 97-113. - ISSN 19971389 (ISSN), DOI 10.17516/1997-1389-0325
Примечания : Cited By :1
Аннотация: Cupriavidus necator B10646 bacterial cells were cultivated in the mode of synthesis of the reserve polyhydroxyalkanoates (PHAs) in the growth medium that contained, in addition to glucose as the main substrate, precursor substrates of the monomers of various monocarboxylic acids - salts of valeric and hexanoic acids, propionate, and ?-butyrolactone. PHA terpolymers and quaterpolymers with different compositions and proportions of monomers were synthesized, and their physicochemical properties were studied. The terpolymers were composed of monomers of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), or 3-hydroxyhexanoate (3HHx) and had the following compositions: P(3HB/3HB/4HHx) and P(3HB/3HV/4HHx). The quaterpolymers had the following composition: P(3HB/3HV/4HB/3HHx). All copolymer samples, regardless of the composition and proportions of monomers, had lower molecular weights and higher polydispersity values compared to the highly crystalline 3-hydroxybutyrate homopolymer, but retained thermal stability properties, with a difference between the melting point and thermal degradation of at least 100-110 °C. The inclusion of 3HV, 4HB, and 3HHx monomers in the C-chain of 3HB caused changes in the crystalline to amorphous phase ratio and a significant decrease in the degree of crystallinity (Cx), which depended on the type of monomers and their contents in the copolymer. The maximum decrease in Cx (9-17 %) was detected in the P(3HB/3HV/4HB) terpolymer and the P(3HB/3HV/4HB/3HHx) quaterpolymer (30-36 %). The study confirms that there is the possibility of synthesizing polymers with various compositions, including new ones, which differ significantly in their basic properties. © Siberian Federal University. All rights reserved.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : SHALAEVA E.E., LISOVSKII G.M., TIKHOMIROV A.A.
Заглавие : SPECTRAL COMPOSITION OF LIGHT AND SOME CHARACTERISTICS OF LEAF MESOSTRUCTURE IN DIFFERENT PLANTS
Колич.характеристики :6 с
Место публикации : SOVIET PLANT PHYSIOLOGY: PLENUM PUBL CORP, 1991. - Vol. 38, Is. 1. - P40-45. - ISSN 0038-5719
Примечания : Cited References: 11
Ключевые слова (''Своб.индексиров.''): radiation spectrum--leaf mesostructure--species specificity
Аннотация: Cross sections and macerates were used to study leaf mesostructure in cucumber, sunflower, and tomato plants formed under controlled environmental conditions at an irradiance of 100 W.m-2 in the red and blue regions of the spectrum. White light served as the control. Three types of response to red light were isolated: the first type is a response not associated with harmful action of the light factor (tomato); the second a response associated with reversible changes of mesostructure (sunflower); and the third a response associated with irreversible changes of leaf mesostructure (cucumber). It is concluded that destructive changes during prolonged action of red radiation on the leaf are mainly associated with degradation of palisade parenchyma, whereas spongy parenchyma suffers less and takes upon itself the main functional role in certain species (sunflower). Species specificity was not clarified in the response of leaf mesostructure to radiation in the blue region of the spectrum.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S. V., Vinogradova O. N., Trusova M. Y.
Заглавие : Specific character of bacterial biodegradation of polyhydroxyalkanoates with different chemical structure in soil
Место публикации : Doklad. Biochem. Biophys.: Maik Nauka Publishing / Springer SBM, 2017. - Vol. 473, Is. 1. - С. 94-97. - ISSN 16076729 (ISSN) , DOI 10.1134/S1607672917010185
Аннотация: The study addresses the influence of the physicochemical properties of the reserve cellular macromolecules (polyhydroxyalkanoates, PHA) with different chemical composition on their biodegradation in the agro-transformed field soil of the Siberian region (Krasnoyarsk Territory, Russia). It was shown that the degradation of the PHA samples depends on the degree of polymer crystallinity (Cx). For the first time, it was shown that the range of PHA-degrading microorganisms differs for each of PHA types. The study defines the primary degraders specific to each PHA type and common to all types of examined polymers. © 2017, Pleiades Publishing, Ltd.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Belyaeva O.G., Plotnikov V.F., Puzyr A.P.
Заглавие : Research of biodegradability of microbial polyhydroxyalkanoates
Место публикации : Dokl. Akad. Nauk: MEZHDUNARODNAYA KNIGA, 1996. - Vol. 350, Is. 5. - С. 707-711. - 5. - ISSN 0869-5652
Примечания : Cited References: 10
Предметные рубрики: HYDROLYTIC DEGRADATION
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila, Natalia, Shishatskaya, Ekaterina
Заглавие : Properties of PHA bi-, ter-, and quarter-polymers containing 4-hydroxybutyrate monomer units
Колич.характеристики :8 с
Коллективы : Russian Science Foundation [17-15-01352]
Место публикации : Int. J. Biol. Macromol.: ELSEVIER SCIENCE BV, 2018. - Vol. 111. - С. 1019-1026. - ISSN 0141-8130, DOI 10.1016/j.ijbiomac.2018.01.130. - ISSN 1879-0003(eISSN)
Примечания : Cited References:39. - The research was supported by the Russian Science Foundation (grant No. 17-15-01352).
Предметные рубрики: ALCALIGENES-FAECALIS T1
COMAMONAS-ACIDOVORANS
ENZYMATIC DEGRADATION
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates--4-hydroxybutyrate monomer units--physicochemical--and mechanical properties
Аннотация: The present study investigates physicochemical, mechanical, and biological properties of polyhydroxyalkanoate (PHA) copolymers containing 4-hydroxybutyrate (4HB) synthesized in Cupriavidus eutrophus B10646 culture. In poly(3-hydroxybutyrate/4-hydroxybutyrate) [P(3HB/4HB)]bipolymers, 4HB varied between 10.4 and 75.0 mol%; in poly(3-hydroxybutyrate/3-hydroxyvalerate/4-hydroxybutyrate) terpolymers, 4HB constituted 28.7-55.6 mol%; and in poly(3-hydroxybutyrate/3-hydroxyvalerate/4-hydroxybutyrate/3-hydroxyhexanoate) quaterpolymers, 4HB varied between 9.3 and 13.3 mol%. The degree of crystallinity of P(3HB/4HB) copolymers decreased consistently with an increase in 4HB content, reaching 38%. The incorporation of 3-hydroxyvalerate and 3-hydroxyhexanoate into copolymers enhanced that effect. The effect of 4HB monomer units on temperature properties of copolymers was exhibited as lowering of the melting temperature and crystallization temperature, which improved the processing-related properties of the copolymers. All copolymers containing 4HB showed enhanced elongation at break compared to poly(3-hydroxybutyrate). Polymer films prepared from PHA5 with different chemical composition had similar microstructure and porosity and had no toxic effect on mouse fibroblast NIH 3 T3 cells, proving their high biocompatibility. (C) 2018 Elsevier B.V. All rights reserved.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Kiselev E., Nemtsev I., Lukyanenko А., Sukovatyi A., Kuzmin A., Ryltseva G., Shishatskaya E.
Заглавие : Properties of degradable polyhydroxyalkanoates with different monomer compositions
Место публикации : Int. J. Biol. Macromol.: Elsevier B.V., 2021. - Vol. 182. - С. 98-114. - ISSN 01418130 (ISSN), DOI 10.1016/j.ijbiomac.2021.04.008
Аннотация: Purpose: To synthesize and investigate polyhydroxyalkanoates (PHAs) with different monomer composition and percentages and polymer films prepared from them. Results: Various PHAs: homopolymer poly-3-hydroxybutyrate P(3HB) and 2-, 3-, and 4-component copolymers comprising various combinations of 3-hydroxybutyrate (3HB), 3-hydroxyvalerate (3HV), 4-hydroxybutyrate (4HB), and 3-hydroxyhexanoate (3HHx) monomers were synthesized under specialized conditions. Relationships were found between the monomer composition of PHAs and their molecular-weight and thermal properties and degree of crystallinity. All copolymers had decreased weight average molecular weights, Mw (to 390–600 kDa), and increased values of polydispersity (3.2–4.6) compared to the P(3HB). PHA copolymers showed different thermal behavior: an insignificant decrease in Tmelt and the presence of the second peak in the melting region and changes in parameters of crystallization and glass transition. At the same time, they retained thermostability, and the difference between Tmelt and Tdegr was at least 100–120 °C. Incorporation of 4HB, 3HV, and 3HHx monomer units into the 3-hydroxybutyrate chain caused changes in the amorphous to crystalline ratio and decreased the degree of crystallinity (Cx) to 20–40%. According to the degree to which the monomers reduced crystallinity, they were ranked as follows: 4HB – 3HHx – 3HV. A unique set of films was produced; their surface properties and physical/mechanical properties were studied as dependent on PHA composition; monomers other than 3-hydroxybutyrate were found to enhance hydrophilicity, surface development, and elasticity of polymer films. Conclusion: An innovative set of PHA copolymers was synthesized and solution-cast films were prepared from them; the copolymers and films were investigated as dependent on polymer chemical composition. Results obtained in the present study contribute to the solution of a critical issue of producing degradable polymer materials. © 2021 Elsevier B.V.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Boersma M.G., van Berkel WJH, Finkelstein Z.I., Golovlev E.L., Baskunov B.P., Vervoort J..., Golovleva L.A., Rietjens IMCM
Заглавие : Preferential oxidative dehalogenation upon conversion of 2-halophenols by Rhodococcus opacus 1G
Колич.характеристики :10 с
Место публикации : FEMS Microbiol. Lett.: ELSEVIER SCIENCE BV, 1999. - Vol. 181, Is. 1. - С. 73-82. - ISSN 0378-1097, DOI 10.1111/j.1574-6968.1999.tb08828.x
Примечания : Cited References: 30
Предметные рубрики: PHENOL HYDROXYLASE
POLYCHLORINATED PHENOLS
MICROBIAL-DEGRADATION
TRICHOSPORON-CUTANEUM
METABOLISM
PURIFICATION
REGIOSELECTIVITY
CHLOROAROMATICS
COMPONENT
BENZOATE
Ключевые слова (''Своб.индексиров.''): phenol hydroxylase--oxidative dehalogenation--2-halophenol--f-19 nmr--rhodococcus opacus
Аннотация: The regiospecificity of hydroxylation of C2-halogenated phenols by Rhodococcus opacus 1G was investigated. Oxidative defluorination at the C2 position ortho with respect to the hydroxyl moiety was preferred over hydroxylation at the non fluorinated C6 position for all 2-fluorophenol compounds studied. Initial hydroxylation of 2,3,5-trichlorophenol resulted in the exclusive formation of 3,5-dichlorocatechol. These results indicate that, in contrast to all other phenol ortho-hydroxylases studied so far, phenol hydroxylase from R. opacus 1G is capable of catalyzing preferential oxidative defluorination but also oxidative dechlorination. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhang, Junyu, Shishatskaya, Ekaterina I., Volova, Tatiana G., da Silva, Luiziana Ferreira, Chen, Guo-Qiang
Заглавие : Polyhydroxyalkanoates (PHA) for therapeutic applications
Колич.характеристики :7 с
Коллективы : Ministry of Sciences and Technology [2016YFB0302504]; National Natural Science Foundation of China [31430003, 31600072, 81503079]; Natural Science Foundation of Jiangxi Province [20161BAB215204]; Russian Science Foundation [17-15-01352]; Tsinghua President Fund [2015THZ10]
Место публикации : Mater. Sci. Eng. C-Mater. Biol. Appl.: ELSEVIER SCIENCE BV, 2018. - Vol. 86. - С. 144-150. - ISSN 0928-4931, DOI 10.1016/j.msec.2017.12.035. - ISSN 1873-0191(eISSN)
Примечания : Cited References:105. - This research was financially supported by a grant from Ministry of Sciences and Technology (Grant No. 2016YFB0302504); grants from National Natural Science Foundation of China (Grant No. 31430003, 31600072 and 81503079); a grant from Natural Science Foundation of Jiangxi Province (Grant No. 20161BAB215204); and a grant from Russian Science Foundation (Grant No. 17-15-01352). Tsinghua President Fund also supported this project (Grant No. 2015THZ10).
Предметные рубрики: TISSUE ENGINEERING APPLICATIONS
3-HYDROXYBUTYRATE METHYL-ESTER
Ключевые слова (''Своб.индексиров.''): pha--therapeutics--tissue engineering--implants--drug delivery--biomedicine
Аннотация: As intracellular carbon and energy storage materials, polyhydroxyalkanoates (PHA) are a diverse biopolyesters synthesized by many bacteria. PHA have been produced in large quantity for various application research including medical implants for approximately 30 years. Many studies demonstrated that PHA are promising implant materials due to their diverse and ascendant mechanical, biodegradable and tissue compatible properties. Importantly, common PHA biodegradation products including oligomers and monomers are also not toxic to the cells and tissues. Pharmaceutical applications of some PHA degradation products also have been reported. So far, no study has been reported to have any carcinogenesis result induced by any PHA or their biodegradation products. All results suggest that PHA could be developed into various bio-implant products.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana, Zhila, Natalia, Kiselev, Evgeniy, Prudnikova, Svetlana, Vinogradova, Olga, Nikolaeva, Elena, Shumilova, Anna, Shershneva, Anna, Shishatskaya, Ekaterina
Заглавие : Poly(3-hydroxybutyrate)/metribuzin formulations: characterization, controlled release properties, herbicidal activity, and effect on soil microorganisms
Колич.характеристики :15 с
Коллективы : Russian Science Foundation [14-26-00039]
Место публикации : Environ. Sci. Pollut. Res.: SPRINGER HEIDELBERG, 2016. - Vol. 23, Is. 23. - С. 23936-23950. - ISSN 0944-1344, DOI 10.1007/s11356-016-7636-7. - ISSN 1614-7499(eISSN)
Примечания : Cited References:41. - This study was supported by the Russian Science Foundation (grant no. 14-26-00039).
Предметные рубрики: METRIBUZIN RELEASE
POLYHYDROXYALKANOATES
POLYMER
MATRIX
PESTICIDES
Ключевые слова (''Своб.индексиров.''): metribuzin--degradable poly-3-hydroxybutyrate--slow-release p(3hb)/met--formulations--release kinetics--agrostis stolonifera--setaria--macrocheata
Аннотация: Slow-release formulations of the herbicide metribuzin (MET) embedded in the polymer matrix of degradable poly-3-hydroxybutyrate [P(3HB)] in the form of microparticles, films, microgranules, and pellets were developed and tested. The kinetics of polymer degradation, MET release, and accumulation in soil were studied in laboratory soil microecosystems with higher plants. The study shows that MET release can be controlled by using different techniques of constructing formulations and by varying MET loading. MET accumulation in soil occurs gradually, as the polymer is degraded. The average P(3HB) degradation rates were determined by the geometry of the formulation, reaching 0.17, 0.12, 0.04, and 0.05 mg/day after 60 days for microparticles, films, microgranules, and pellets, respectively. The herbicidal activities of P(3HB)/MET formulations and commercial formulation Sencor Ultra were tested on the Agrostis stolonifera and Setaria macrocheata plants. The parameters used to evaluate the herbicidal activity were plant density and the weight of fresh green biomass measured at days 10, 20, and 30 after sowing. All P(3HB)/MET formulations had pronounced herbicidal activity, which varied depending on MET loading and the stage of the experiment. In the early phases of the experiment, the herbicidal effect of P(3HB)/MET formulations with the lowest MET loading (10 %) was comparable with that of the commercial formulation. The herbicidal effect of P(3HB)/MET formulations with higher MET loadings (25 and 50 %) at later stages of the experiment were stronger than the effect of Sencor Ultra.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Plotnikov V.F., Shishatskaya E.I., Mironov P.V., Vasil'ev A.D.
Заглавие : Physicochemical properties of two-component polyhydroxyalkanoates [Poly(3HB/3HV)]
Колич.характеристики :9 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2004. - Vol. 49, Is. 6. - P1038-1046. - ISSN 0006-3029
Примечания : Cited References: 21
Предметные рубрики: BACTERIAL POLYHYDROXYALKANOATES
POLYESTERS
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates--copolymers of hydroxybutyrate and hydroxyvalerate--structure--physicochemical properties
Аннотация: A series of two-component polyhydroxyalkanoates consisting of hydroxybutyrate and hydroxyvalerate monomer at different ratios were synthesized using the bacterium Ralstonia eutropha B5786. The properties of polyhydroxyalkanoates were compared with those of the homopolymer of hydroxybutyric acid by X-ray structure analysis, IR spectroscopy, differential scanning calorimetry, and viscosimetry. With an increase in the molar fraction of hydroxyvalerate, an equalization of the ratio of the crystalline and amorphous phases in the copolymer was observed. The degree of crystallinity of the polymer decreased from 70-80 to 45-50%; in the range of an increase in the hydroxyvalerate molar fraction from several to 25-30 mol %, the dependence was linear. The temperature characteristics, the melting temperature (T-m), and the degradation temperature (T-d) were lower in polyhydroxyalkanoates than in polyhydroxybutyrate, for which T-m and T-d were 168-170 and 260-265degreesC, respectively. In the copolymer, as the molar fraction of hydroxyvalerate grew, both parameters decreased. In the range of variation of monomer ratio studied, they decreased to 150-160 and 200-220degreesC, respectively. No distinct correlation between the composition of the polymer and its molecular mass was found.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Vinogradova O. N., Zhila N. O., Kiselev E. G., Peterson I. V., Vasil’ev A. D., Sukovatyi A. G., Shishatskaya E. I.
Заглавие : Physicochemical properties of multicomponent polyhydroxyalkanoates: Novel aspects
Место публикации : Polym. Sci. Ser. A: Maik Nauka-Interperiodica Publishing, 2017. - Vol. 59, Is. 1. - С. 98-106. - ISSN 0965545X (ISSN) , DOI 10.1134/S0965545X17010163
Ключевые слова (''Своб.индексиров.''): glycols--medical problems--melting--molecular mass--monomers--polymers--chemical compositions--degree of crystallinity--degrees of crystallinity--multi-component polyhydroxyalkanoates--physicochemical property--polyhydroxyalkanoates--temperature intervals--thermal degradation temperatures--organic polymers
Аннотация: The physicochemical properties such as the degree of crystallinity and temperature and molecularmass characteristics of a number of polyhydroxyalkanoates of various chemical composition synthesized on a complex carbon substrate by bacteria Cupriavidus eutrophus В10646 have been investigated. Two-, three-, and four-component copolymer samples have different sets and ratios of monomers with various lengths of carbon chains: 3-hydroxybutyrate (3HB), 4-hydroxybutyrate (4HB), 3-hydroxyvalerate (3HV), 3-hydroxyhexanoate (3HH), 3-hydroxy-4-methyl valerate (3H4MV), and diethylene glycol (DEG). It has been shown that weight-average molar mass Мw and polydispersity vary in a wide range with no correlation existing with the composition of copolymer polyhydroxyalkanoates and that thermal stability is preserved in the temperature interval between the melting temperature and the thermal degradation temperature from 100 to 120–140°С. The composition and ratio of monomers most notably affect the degree of crystallinity of polyhydroxyalkanoates. Significant differences between the degrees of crystallinity of three- and four-component polyhydroxyalkanoates have been found for the first time. The degree of crystallinity for copolymers P(3HB/3HV/4HB) is 9–22%, and the degree of crystallinity for copolymers P(3HB/3HV/3HH) and P(3HB/3GV/3H4MV) is 41–63%; this value is close to the degree of crystallinity for diblock copolymers P(3HB)/DEG, which is 56–69%. For the four-component copolymers P(3HB/3GV/4HB/3HH), the degree of crystallinity is 30–41%. The values of Мw for the copolymers P(3HB/DEG) are inhomogeneous and the polymers contain fractions uneven with respect to molecular mass: a high-molecular-mass polymer (Мw from 2700 to 4900 kDa) and a low-molecular-mass polymer (Мw = 46–167 kDa). For the copolymers P(3HB)/DEG and P(3HB/3HV/3H4MV), two peaks are observed in the region of melting with the gap between these peaks being 4–20°С. All of the types of copolymer samples, regardless of the monomer ratio, show an increase in elongation at break against the background of a decrease in tensile stress and Young’s modulus, with these effects being pronounced to different extents. On the whole, the properties of multicomponent polyhydroxyalkanoates differ appreciably. © 2017, Pleiades Publishing, Ltd.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Manukovski N.S., Teremova M.I., Gurevich Y.L., Pan'Kova I.M.
Заглавие : Phenol and Naphthalene degradation by mixed culture of microorganisms
Место публикации : Studies in Environmental Science. - 1991. - Vol. 42, Is. C. - P155-163. - ISSN 01661116 (ISSN) , DOI 10.1016/S0166-1116(08)70323-6
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