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1.


   
    Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays / A. Davydova, V. Krasitskaya, P. Vorobjev [et al.] // RSC Adv. - 2020. - Vol. 10, Is. 54. - P32393-32399, DOI 10.1039/d0ra05117a. - Cited References:33. - The work was supported by the Russian Science Foundation (grant #16-14-10296), Russian State funded budget projects #AAAA-A17-117020210021-7 to ICBFM SB RAS and #AAAA-A19-119031890015-0 to IBP SB RAS. . - ISSN 2046-2069
РУБ Chemistry, Multidisciplinary
Рубрики:
DNA APTAMER
   RNA APTAMER
   OBELIN
   PURIFICATION
   EXPRESSION
   SEQUENCES
Аннотация: We report a novel bioluminescent aptasensor, which consists of 2 '-F-RNA aptamer modules joined into a bi-specific aptamer construct. One aptamer module binds the analyte, then after structural rearrangement the second module recruits non-covalently Ca2+-dependent photoprotein obelin from the solution, thus providing a bioluminescent signal. This concept allows using free protein as a reporter, which brings such advantages as no need for aptamer-protein conjugation, a possibility of thermal re-folding of aptamer component with no harm to a protein, and simpler detection protocol. We developed the new 2 '-F-RNA aptamer for obelin, and proposed the strategy for engineering structure-switching bi-modular aptamer constructs which bind the analyte and the obelin in a sequential manner. With the use of hemoglobin as a model analyte, we showed the feasibility of utilizing the aptasensor in a fast and straightforward bioluminescent microplate assay. With a proper design of a secondary structure, this strategy of aptasensor engineering might be further extended to bi-specific aptamer-based bioluminescent sensors for other analytes of interest.

WOS
Держатели документа:
SB RAS, Inst Chem Biol & Fundamental Med, Novosibirsk 630090, Russia.
SB RAS, Inst Biophys, Fed Res Ctr, Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.
Novosibirsk State Univ, Pimgova St 2, Novosibirsk 630090, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Davydova, Anna; Krasitskaya, Vasilisa; Vorobjev, Pavel; Timoshenko, Valentina; Tupikin, Alexey; Kabilov, Marsel; Frank, Ludmila; Venyaminova, Alya; Vorobyeva, Mariya; Russian Science FoundationRussian Science Foundation (RSF) [16-14-10296]; Russian State funded budget projects [AAAA-A17-117020210021-7, AAAA-A19-119031890015-0]

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2.


   
    The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation / V. V. Krasitskaya, N. S. Goncharova, V. V. Biriukov [et al.] // Photochem. Photobiol. - 2020, DOI 10.1111/php.13274. - Cited References:25. - This work has been supported by the Russian Foundation for Basic Research (RFBR) under the grant no 18-38-00531. . - Article in press. - ISSN 0031-8655. - ISSN 1751-1097
РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
CARDIAC TROPONIN-I
   BIOLUMINESCENCE
   IMMUNOASSAY
   APTASENSOR
   DIAGNOSIS
Аннотация: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.

WOS
Держатели документа:
Fed Res Ctr KSC SB RAS, Inst Biophys SB RAS, Krasnoyarsk, Russia.
Siberian Fed Univ, Krasnoyarsk, Russia.
Inst Chem Biol & Fundamental Med SB RAS, Novosibirsk, Russia.
Fed Res Ctr KSC SB RAS, Kirensky Inst Phys, Krasnoyarsk, Russia.

Доп.точки доступа:
Krasitskaya, Vasilisa V.; Goncharova, Natalia S.; Biriukov, Vladislav V.; Bashmakova, Eugenia E.; Kabilov, Marsel R.; Baykov, Ivan K.; Sokolov, Aleksey E.; Frank, Ludmila A.; Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [18-38-00531]

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3.


   
    Antimicrobial and antiradical activity of individual fractions of essential oil from seeds of heracleum dissectum ledeb. Of Siberian Region / A. A. Efremov, I. D. Zykova, N. S. Korosteleva // Khimiya Rastitel'nogo Syr'ya. - 2020. - Is. 2. - С. 79-85, DOI 10.14258/JCPRM.2020027029 . - ISSN 1029-5151
   Перевод заглавия: АНТИМИКРОБНАЯ И АНТИРАДИКАЛЬНАЯ АКТИВНОСТЬ ОТДЕЛЬНЫХ ФРАКЦИЙ ЭФИРНОГО МАСЛА ПЛОДОВ HERACLEUM DISSECTUM LEDEB. СИБИРСКОГО РЕГИОНА
Кл.слова (ненормированные):
2 -- 2-diphenyl-1-picrylhydrazyl -- Antimicrobial activity -- Antiradical activity -- Beans -- Essential oil -- Heracleum dissectum Ledeb
Аннотация: By the method of exhaustive hydroponically obtained essential oil from beans of Heracleum dissectum Ledeb., growing in the Krasnoyarsk region. Separate fractions of oil were obtained: the first after 45 minutes from the beginning of distillation, the second – after 2 hours, the third-after 5 hours, the fourth fraction was collected after the end of hydro-distillation. The component composition of both whole essential oil and its separate fractions was studied. The main components are octyl acetate (60.0%), octyl-2-methylpropanoate (10.2%), n-hexyl-2-methylbutanoate (9.0%). The main amount of octyl acetate (64.7%) is concentrated in the first fraction of the oil. The antimicrobial activity of various fractions of essential oil of borscht dissected against strains of opportunistic microorganisms: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus 209p, MRSA, Proteus vulgaris. It was found that, depending on the duration of isolation, the antimicrobial activity of essential oil fractions in relation to Staphylococcus aureus 209p, MRSA and Pseudomonas aeruginosa decreases, and in relation to Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris increases. The most pronounced inhibitory effect of the third and fourth fractions of essential oil against Klebsiella pneumonia. The antiradical activity of all studied samples of borscht essential oil dissected in reaction with stable free 2,2-diphenyl-1-picrylhydrazyl radical was established. The first fraction showed minimal antiradical activity (15.1%), the fourth – maximum (49.2%). © 2020 Altai State University. All rights reserved.

Scopus
Держатели документа:
Siberian Federal University, pr. Svobodnyy, 79, Krasnoyarsk, 660049, Russian Federation
Special Design and Technology Bureau “Science”, Federal Research Center of the KSC SB RAS, Akademgorodok, 50/45, Krasnoyarsk, 660036, Russian Federation
Institute of Biophysics, Federal Research Center, KSC SB RAS, Akademgorodok, 50/50, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Efremov, A. A.; Zykova, I. D.; Korosteleva, N. S.

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4.


   
    Deeper waters are changing less consistently than surface waters in a global analysis of 102 lakes / R. M. Pilla, C. E. Williamson, B. V. Adamovich [et al.] // Sci Rep. - 2020. - Vol. 10, Is. 1. - Ст. 20514, DOI 10.1038/s41598-020-76873-x. - Cited References:87. - This work was conceived at the Global Lake Ecological Observatory Network (GLEON), and benefited from continued participation and travel support from GLEON. This manuscript is dedicated to the late Alon Rimmer and Karl Havens, who provided data and contributed to earlier versions of this manuscript. Funding in support of this work came from the following sources: Belarus Republican Foundation for Fundamental Research; IGB Long-Term Research; the European Commission within the MANTEL project; the DFG within the LimnoScenES project (AD 91/22-1); OLA-IS, AnaEE-France, INRAE of Thonon-les-Bains, CIPEL, SILA, CISALB; Universidad del Valle de Guatemala; Archbold Biological Station; the Oklahoma Department of Wildlife Conservation, the Oklahoma Water Resources Board, the Grand River Dam Authority, the US Army Corps of Engineers, and the City of Tulsa; the Ministry of Business, Innovation, and Employment (UOW X1503); the Natural Environment Research Council of the UK; the IGB's International Postdoctoral Fellowship; NSERC, Canada Foundation for Innovation, Canada Research Chairs, Province of Saskatchewan; University of Regina; Queen's University Belfast; Natural Environment Research Council; US-NSF, California Air Resources Board, NASA, and US National Park Service; the Ministry of Higher Education and Research (projects No FZZE-2020-0026; No FZZE-2020-0023) and RSCF 20-64-46003; US National Science Foundation Long Term Research in Environmental Biology program (DEB-1242626); the Environmental Agency of Verona; US National Science Foundation, the Gordon and Betty Moore Foundation, the Mellon Foundation, and the University of Washington; KMFRI, LVEMP, University of Innsbruck, OeAD, IFS, and LVFO-EU; Waikato Regional Council and Bay of Plenty Regional Council; Swedish Environmental Protection Agency and the Swedish Infrastructure for Ecosystem Sciences; US National Science Foundation grants DEB-1754276 and DEB-1950170. We thank J. Klug, P. McIntyre, H. Swain, K. Tominaga, A. Voutilainen, and L. Winslow for their feedback on early drafts that substantially improved this manuscript. Additional detailed acknowledgements can be found in the Supplementary Information online. . - ISSN 2045-2322
РУБ Multidisciplinary Sciences
Рубрики:
DISSOLVED ORGANIC-CARBON
   LONG-TERM CHANGES
   CLIMATE-CHANGE
   OXYGEN
Аннотация: Globally, lake surface water temperatures have warmed rapidly relative to air temperatures, but changes in deepwater temperatures and vertical thermal structure are still largely unknown. We have compiled the most comprehensive data set to date of long-term (1970-2009) summertime vertical temperature profiles in lakes across the world to examine trends and drivers of whole-lake vertical thermal structure. We found significant increases in surface water temperatures across lakes at an average rate of+0.37 degrees C decade(-1), comparable to changes reported previously for other lakes, and similarly consistent trends of increasing water column stability (+0.08 kg m(-3) decade(-1)). In contrast, however, deepwater temperature trends showed little change on average (+0.06 degrees C decade(-1)), but had high variability across lakes, with trends in individual lakes ranging from -0.68 degrees C decade(-1) to+0.65 degrees C decade(-1). The variability in deepwater temperature trends was not explained by trends in either surface water temperatures or thermal stability within lakes, and only 8.4% was explained by lake thermal region or local lake characteristics in a random forest analysis. These findings suggest that external drivers beyond our tested lake characteristics are important in explaining long-term trends in thermal structure, such as local to regional climate patterns or additional external anthropogenic influences.

WOS
Держатели документа:
Miami Univ, Dept Biol, Oxford, OH 45056 USA.
Belarusian State Univ, Fac Biol, Minsk, BELARUS.
Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Ecosyst Res, Berlin, Germany.
Free Univ Berlin, Berlin, Germany.
INRAE, CARRTEL, Thonon Les Bains, France.
Univ Nevada, Global Water Ctr, Reno, NV 89557 USA.
Uppsala Univ, Dept Ecol & Genet Limnol, Uppsala, Sweden.
Univ Montana, Flathead Lake Biol Stn, Polson, MT 59860 USA.
Univ Valle Guatemala, Inst Investigacones, Guatemala City, Guatemala.
Univ Innsbruck, Res Dept Limnol Mondsee, Mondsee, Austria.
Florida Int Univ, Dept Biol Sci, Miami, FL 33199 USA.
Natl Pk Serv, Crater Lake Natl Pk, Crater Lake, OR USA.
Univ Oklahoma, Dept Biol, Plankton Ecol & Limnol Lab, Norman, OK 73019 USA.
Univ Oklahoma, Geog Ecol Grp, Norman, OK 73019 USA.
Griffith Univ, Australian Rivers Inst, Nathan, Qld, Australia.
Univ Florida, Florida Sea Grant & UF IFAS, Gainesville, FL USA.
Univ Oslo, Dept Biosci, Oslo, Norway.
IISD Expt Lake Area Inc, Winnipeg, MB, Canada.
Finnish Environm Inst SYKE, Freshwater Ctr, Helsinki, Finland.
Univ Eastern Finland, Dept Environm & Biol Sci, Joensuu, Finland.
Eawag Swiss Fed Inst Aquat Sci & Technol, Dept Aquat Ecol, Dubendorf, Switzerland.
CSIRO, Land & Water, Canberra, ACT, Australia.
Univ Stirling, Biol & Environm Sci, Stirling, Scotland.
Laurentian Univ, Cooperat Freshwater Ecol Unit, Ramsey Lake Rd, Sudbury, ON, Canada.
Univ Minnesota, Itasca Biol Stn & Labs, Lake Itasca, MN USA.
Univ Regina, Inst Environm Change & Soc, Regina, SK, Canada.
Queens Univ Belfast, Inst Global Food Secur, Belfast, Antrim, North Ireland.
Univ Appl Sci & Arts Southern Switzerland, Dept Environm Construct & Design, Canobbio, Switzerland.
Fed Agcy Water Management, Mondsee, Austria.
UK Ctr Ecol & Hydrol, Lake Ecosyst Grp, Lancaster, England.
Univ Calif Santa Barbara, Bren Sch Environm Sci & Management, Santa Barbara, CA 93106 USA.
Ryerson Univ, Dept Chem & Biol, Toronto, ON, Canada.
Univ Hamburg, Dept Biol, Hamburg, Germany.
Irkutsk State Univ, Inst Biol, Irkutsk, Russia.
Univ Liege, Liege, Belgium.
SUNY Coll New Paltz, Dept Biol, New Paltz, NY 12561 USA.
Israel Oceanog & Limnol Res, Kinneret Limnol Lab, Migdal, Israel.
CNR Water Res Inst, Verbania, Italy.
Ontario Minist Environm Conservat & Parks, Dorset Environm Sci Ctr, Dorset, ON, Canada.
Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA.
Fdn Edmund Mach FEM, Dept Sustainable Agroecosyst & Bioreso, Res & Innovat Ctr, San Michele All Adige, Italy.
Univ Maine, Climate Change Inst, Orono, ME USA.
Univ Laval, Ctr Etud Nord, Quebec City, PQ, Canada.
Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA.
Eawag Swiss Fed Inst Aquat Sci & Technol, Surface Waters Res & Management, Kastanienbaum, Switzerland.
Tech Univ Kenya, Dept Geosci & Environm, Nairobi, Kenya.
Univ Innsbruck, Dept Ecol, Innsbruck, Austria.
Univ Konstanz, Limnol Inst, Constance, Germany.
Dickinson Coll, Dept Environm Sci, Carlisle, PA 17013 USA.
Vrije Univ Brussel, Dept Hydrol & Hydraul Engn, Brussels, Belgium.
Eidgenoss Tech Hsch Zurich, Inst Atmospher & Climate Sci, Zurich, Switzerland.
Natl Inst Water & Atmospher Res, Hamilton, New Zealand.
Univ Alberta, Dept Biol Sci, Edmonton, AB, Canada.
Russian Acad Sci, Krasnoyarsk Sci Ctr, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia.

Доп.точки доступа:
Pilla, Rachel M.; Williamson, Craig E.; Adamovich, Boris V.; Adrian, Rita; Anneville, Orlane; Chandra, Sudeep; Colom-Montero, William; Devlin, Shawn P.; Dix, Margaret A.; Dokulil, Martin T.; Gaiser, Evelyn E.; Girdner, Scott F.; Hambright, K. David; Hamilton, David P.; Havens, Karl; Hessen, Dag O.; Higgins, Scott N.; Huttula, Timo H.; Huuskonen, Hannu; Isles, Peter D. F.; Joehnk, Klaus D.; Jones, Ian D.; Keller, Wendel Bill; Knoll, Lesley B.; Korhonen, Johanna; Kraemer, Benjamin M.; Leavitt, Peter R.; Lepori, Fabio; Luger, Martin S.; Maberly, Stephen C.; Melack, John M.; Melles, Stephanie J.; Muller-Navarra, D. C.; Pierson, Don C.; Pislegina, Helen V.; Plisnier, Pierre-Denis; Richardson, David C.; Rimmer, Alon; Rogora, Michela; Rusak, James A.; Sadro, Steven; Salmaso, Nico; Saros, Jasmine E.; Saulnier-Talbot, Emilie; Schindler, Daniel E.; Schmid, Martin; Shimaraeva, Svetlana V.; Silow, Eugene A.; Sitoki, Lewis M.; Sommaruga, Ruben; Straile, Dietmar; Strock, Kristin E.; Thiery, Wim; Timofeyev, Maxim A.; Verburg, Piet; Vinebrooke, Rolf D.; Weyhenmeyer, Gesa A.; Zadereev, Egor; Belarus Republican Foundation for Fundamental Research; IGB Long-Term Research; European CommissionEuropean CommissionEuropean Commission Joint Research Centre; DFGGerman Research Foundation (DFG) [AD 91/22-1]; OLA-IS; AnaEE-France; INRAE of Thonon-les-Bains; CIPEL; SILA; CISALB; Universidad del Valle de Guatemala; Archbold Biological Station; Oklahoma Department of Wildlife Conservation; Oklahoma Water Resources Board; Grand River Dam Authority; US Army Corps of EngineersUnited States Department of Defense; City of Tulsa; Ministry of Business, Innovation, and EmploymentNew Zealand Ministry of Business, Innovation and Employment (MBIE) [UOW X1503]; Natural Environment Research Council of the UKNERC Natural Environment Research Council; IGB's International Postdoctoral Fellowship; NSERCNatural Sciences and Engineering Research Council of Canada; Canada Foundation for InnovationCanada Foundation for InnovationCGIAR; Canada Research ChairsCanada Research ChairsCGIAR; Province of Saskatchewan; University of Regina; Queen's University Belfast; Natural Environment Research CouncilNERC Natural Environment Research Council; US-NSFNational Science Foundation (NSF); California Air Resources Board; NASANational Aeronautics & Space Administration (NASA); US National Park Service; Ministry of Higher Education and ResearchMinistry of Higher Education & Scientific Research (MHESR) [FZZE-2020-0026, FZZE-2020-0023]; RSCFRussian Science Foundation (RSF) [20-64-46003]; US National Science Foundation Long Term Research in Environmental Biology program [DEB-1242626]; Environmental Agency of Verona; US National Science FoundationNational Science Foundation (NSF); Gordon and Betty Moore FoundationGordon and Betty Moore Foundation; Mellon Foundation; University of WashingtonUniversity of Washington; KMFRI; LVEMP; University of Innsbruck; OeAD; IFSInternational Foundation for Science; LVFO-EU; Waikato Regional Council; Bay of Plenty Regional Council; Swedish Environmental Protection Agency; Swedish Infrastructure for Ecosystem Sciences; US National Science FoundationNational Science Foundation (NSF) [DEB-1754276, DEB-1950170]

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5.


   
    Deeper waters are changing less consistently than surface waters in a global analysis of 102 lakes / R. M. Pilla, C. E. Williamson, B. V. Adamovich [et al.] // Sci. Rep. - 2020. - Vol. 10, Is. 1. - Ст. 20514, DOI 10.1038/s41598-020-76873-x . - ISSN 2045-2322
Аннотация: Globally, lake surface water temperatures have warmed rapidly relative to air temperatures, but changes in deepwater temperatures and vertical thermal structure are still largely unknown. We have compiled the most comprehensive data set to date of long-term (1970–2009) summertime vertical temperature profiles in lakes across the world to examine trends and drivers of whole-lake vertical thermal structure. We found significant increases in surface water temperatures across lakes at an average rate of + 0.37 °C decade?1, comparable to changes reported previously for other lakes, and similarly consistent trends of increasing water column stability (+ 0.08 kg m?3 decade?1). In contrast, however, deepwater temperature trends showed little change on average (+ 0.06 °C decade?1), but had high variability across lakes, with trends in individual lakes ranging from ? 0.68 °C decade?1 to + 0.65 °C decade?1. The variability in deepwater temperature trends was not explained by trends in either surface water temperatures or thermal stability within lakes, and only 8.4% was explained by lake thermal region or local lake characteristics in a random forest analysis. These findings suggest that external drivers beyond our tested lake characteristics are important in explaining long-term trends in thermal structure, such as local to regional climate patterns or additional external anthropogenic influences. © 2020, The Author(s).

Scopus
Держатели документа:
Department of Biology, Miami University, Oxford, OH, United States
Faculty of Biology, Belarusian State University, Minsk, Belarus
Department of Ecosystems Research, Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Berlin, Germany
Freie Universitat Berlin, Berlin, Germany
CARRTEL, INRAE, Thonon-les-Bains, France
Global Water Center, University of Nevada, Reno, NV, United States
Department of Ecology and Genetics/Limnology, Uppsala University, Uppsala, Sweden
Flathead Lake Biological Station, University of Montana, Polson, MT, United States
Instituto de Investigacones, Universidad del Valle de Guatemala, Guatemala, Guatemala
Research Department for Limnology Mondsee, University of Innsbruck, Mondsee, Austria
Department of Biological Sciences, Florida International University, Miami, FL, United States
Crater Lake National Park, U.S. National Park Service, Crater Lake, OR, United States
Department of Biology, Plankton Ecology and Limnology Lab and Geographical Ecology Group, University of Oklahoma, Norman, OK, United States
Australian Rivers Institute, Griffith University, Nathan, Australia
Florida Sea Grant and UF/IFAS, University of Florida, Gainesville, FL, United States
Department of Biosciences, University of Oslo, Oslo, Norway
IISD Experimental Lake Area Inc, Winnipeg, MB, Canada
Freshwater Center, Finnish Environment Institute SYKE, Helsinki, Finland
Department of Environmental and Biological Sciences, University of Eastern Finland, Joensuu, Finland
Department of Aquatic Ecology, Eawag: Swiss Federal Institute of Aquatic Science and Technology, Dubendorf, Switzerland
Land and Water, CSIRO, Canberra, Australia
Biological and Environmental Sciences, University of Stirling, Stirling, United Kingdom
Cooperative Freshwater Ecology Unit, Laurentian University, Ramsey Lake Road, Sudbury, ON, Canada
Itasca Biological Station and Laboratories, University of Minnesota, Lake Itasca, MN, United States
Institute of Environmental Change and Society, University of Regina, Regina, SK, Canada
Institute for Global Food Security, Queen’s University Belfast, Belfast Co., Antrim, United Kingdom
Department for Environment, Constructions and Design, University of Applied Sciences and Arts of Southern Switzerland, Canobbio, Switzerland
Federal Agency for Water Management AT, Mondsee, Austria
Lake Ecosystems Group, UK Centre for Ecology & Hydrology, Lancaster, United Kingdom
Bren School of Environmental Science and Management, University of California, Santa Barbara, CA, United States
Department of Chemistry and Biology, Ryerson University, Toronto, ON, Canada
Department of Biology, University of Hamburg, Hamburg, Germany
Institute of Biology, Irkutsk State University, Irkutsk, Russian Federation
University of Liege, Liege, Belgium
Department of Biology, SUNY New Paltz, New Paltz, NY, United States
The Kinneret Limnological Laboratory, Israel Oceanographic and Limnological Research, Migdal, Israel
CNR Water Research Institute, Verbania Pallanza, Italy
Dorset Environmental Science Centre, Ontario Ministry of the Environment, Conservation, and Parks, Dorset, ON, Canada
Department of Environmental Science and Policy, University of California Davis, Davis, CA, United States
Department of Sustainable Agro-Ecosystems and Bioresources, Research and Innovation Centre, Fondazione Edmund Mach (FEM), San Michele All’Adige, Italy
Climate Change Institute, University of Maine, Orono, ME, United States
Centre D’Etudes Nordiques, Universite Laval, Quebec, QC, Canada
School of Aquatic and Fishery Sciences, University of Washington, Seattle, WA, United States
Surface Waters-Research and Management, Eawag: Swiss Federal Institute of Aquatic Science and Technology, Kastanienbaum, Switzerland
Department of Geosciences and the Environment, The Technical University of Kenya, Nairobi, Kenya
Department of Ecology, University of Innsbruck, Innsbruck, Austria
Limnological Institute, University of Konstanz, Konstanz, Germany
Department of Environmental Science, Dickinson College, Carlisle, PA, United States
Department of Hydrology and Hydraulic Engineering, Vrije Universiteit Brussel, Brussels, Belgium
Institute for Atmospheric and Climate Science, Eidgenossische Technische Hochschule Zurich, Zurich, Switzerland
National Institute of Water and Atmospheric Research, Hamilton, New Zealand
Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada
Institute of Biophysics, Krasnoyarsk Scientific Center Siberian Branch of the Russian Academy of Sciences, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Pilla, R. M.; Williamson, C. E.; Adamovich, B. V.; Adrian, R.; Anneville, O.; Chandra, S.; Colom-Montero, W.; Devlin, S. P.; Dix, M. A.; Dokulil, M. T.; Gaiser, E. E.; Girdner, S. F.; Hambright, K. D.; Hamilton, D. P.; Havens, K.; Hessen, D. O.; Higgins, S. N.; Huttula, T. H.; Huuskonen, H.; Isles, P. D.F.; Joehnk, K. D.; Jones, I. D.; Keller, W. B.; Knoll, L. B.; Korhonen, J.; Kraemer, B. M.; Leavitt, P. R.; Lepori, F.; Luger, M. S.; Maberly, S. C.; Melack, J. M.; Melles, S. J.; Muller-Navarra, D. C.; Pierson, D. C.; Pislegina, H. V.; Plisnier, P. -D.; Richardson, D. C.; Rimmer, A.; Rogora, M.; Rusak, J. A.; Sadro, S.; Salmaso, N.; Saros, J. E.; Saulnier-Talbot, E.; Schindler, D. E.; Schmid, M.; Shimaraeva, S. V.; Silow, E. A.; Sitoki, L. M.; Sommaruga, R.; Straile, D.; Strock, K. E.; Thiery, W.; Timofeyev, M. A.; Verburg, P.; Vinebrooke, R. D.; Weyhenmeyer, G. A.; Zadereev, E.

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6.


   
    Benefits of biophysical tests of saliva for athletic functionality rating tests / A. M. Vyshedko [и др.] // Teor. Prakt. Fiz. Kult. - 2019. - Vol. 2019, Is. 7. - С. 65-67 . - ISSN 0040-3601
Кл.слова (ненормированные):
Antioxidant activity rate -- Bioluminescence test -- Chemoluminescence test -- Elite sports -- Residual luminescence -- Saliva
Аннотация: Modern physical training systems give a high priority to the training process individualizing elements that may be efficient enough only when supported by objective tests to rate the bodily responses to physical workloads. Moreover, success in modern professional sports is impossible unless supported by the newest medical/ biological findings and technologies in the health rating and forecast domains, with a special role played by the modern biophysical research of the cellular- and molecular-level biological processes. The study was designed to assess benefits of biophysical analysis of saliva for the athletic functionality rating tests. Sampled for the purposes of the study were athletes of different skill levels (from Class I to Honorary Masters of Sport) versus the untrained students, with the saliva sampled prior to and after physical trainings by bioluminescence and chemoluminescence test methods. The test data were processed by Statistica 10 (made by StatSoft Inc., the US) toolkit to produce median values (Me) and inter-quartile distribution (C25-C75 percentile) rates. The Mann-Whitney nonparametric U-test of the null hypothesis was used to rate differences of the independent variables, with the statistical difference rated meaningful at p<0.05. The bioluminescence tests found the higher ferment activity of saliva in the athletes’ versus untrained students, with the ferment activity in the bioluminescence tests also tested to grow after physical workloads in the highly-skilled athletes and fall in the untrained students. The study found the saliva antioxidant system functional intensity being athletic skills dependent. Therefore, the study has demonstrated benefits of the biophysical (bioluminescence and chemoluminescence) tests of saliva for the athletic functionality rating tests in the physical workload design and management initiatives. © 2019, Teoriya i praktika fizicheskoy kul'tury i sporta. All rights reserved.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Research Institute of Medical Problems of the North, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Vyshedko, A. M.; Stepanova, L. V.; Kolenchukova, O. A.; Kratasyuk, V. A.

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7.


   
    Battle of GLP-1 delivery technologies / M. Yu [et al.] // Adv. Drug Deliv. Rev. - 2018, DOI 10.1016/j.addr.2018.07.009 . - ISSN 0169-409X
Кл.слова (ненормированные):
Albumin fusion -- Exenatide -- Fatty acid conjugate -- Fc fusion -- GLP-1 receptor agonist -- Half-life -- Peptide delivery -- Pharmacokinetics
Аннотация: Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) belong to an important therapeutic class for treatment of type 2 diabetes. Six GLP-1 RAs, each utilizing a unique drug delivery strategy, are now approved by the Food and Drug Administration (FDA) and additional, novel GLP-1 RAs are still under development, making for a crowded marketplace and fierce competition among the manufacturers of these products. As rapid elimination is a major challenge for clinical application of GLP-1 RAs, various half-life extension strategies have been successfully employed including sequential modification, attachment of fatty-acid to peptide, fusion with human serum albumin, fusion with the fragment crystallizable (Fc) region of a monoclonal antibody, sustained drug delivery systems, and PEGylation. In this review, we discuss the scientific rationale of the various half-life extension strategies used for GLP-1 RA development. By analyzing and comparing different approved GLP-1 RAs and those in development, we focus on assessing how half-life extending strategies impact the pharmacokinetics, pharmacodynamics, safety, patient usability and ultimately, the commercial success of GLP-1 RA products. We also anticipate future GLP-1 RA development trends. Since similar drug delivery strategies are also applied for developing other therapeutic peptides, we expect this case study of GLP-1 RAs will provide generalizable concepts for the rational design of therapeutic peptides products with extended duration of action. © 2018 Elsevier B.V.

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Держатели документа:
Department of Pharmaceutical Sciences, College of Pharmacy, University of Michigan, 428 Church St, Ann Arbor, MI, United States
Amneal Pharmaceuticals, 50 Horseblock Rd, Brookhaven, NY, United States
Siberian Federal University, 79 Svobodnuy Ave, Krasnoyarsk, Russian Federation
Institute of Biophysics SBRAS, 50 Akademgorodok, Russian Federation
Biointerfaces Institute, NCRC, 2800 Plymouth Rd, Ann Arbor, MI, United States
Department of Biomedical Engineering, 2200 Bonisteel Blvd, Ann Arbor, MI, United States

Доп.точки доступа:
Yu, M.; Benjamin, M. M.; Srinivasan, S.; Morin, E. E.; Shishatskaya, E. I.; Schwendeman, S. P.; Schwendeman, A.

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8.


   
    Bioluminescent SNP genotyping technique: Development and application for detection of melanocortin 1 receptor gene polymorphisms / E. E. Bashmakova [et al.] // Talanta. - 2018. - Vol. 189. - P111-115, DOI 10.1016/j.talanta.2018.06.057 . - ISSN 0039-9140
Кл.слова (ненормированные):
Ca2+-regulated photoprotein obelin -- Genotyping -- Melanocortin 1 receptor gene -- Single nucleotide polymorphisms (SNP) -- Bioluminescence -- Clinical research -- Curricula -- Diagnosis -- Genes -- Oncology -- Biomedical research -- Clinical characteristics -- Development and applications -- Genotyping -- Healthy individuals -- Photoproteins -- Receptor genes -- Single-nucleotide polymorphisms -- Dermatology
Аннотация: SNP genotyping based on the reaction of specific primer extension with the following bioluminescent detection of its products was shown to be potentially applicable for biomedical exploration. The paper describes its elaboration and first application in extensive biomedical research concerning MC1R gene variants’ frequency and associations with clinical characteristics in melanoma patients of Eastern Siberia (Krasnoyarsk region, Russia). Polymorphisms rs 1805007 (R151C), rs 1805008 (R160W), and rs 1805009 (D294H) were detected in 174 DNA samples from patients with histologically proved diagnosis of cutaneous melanoma and in 200 samples from healthy individuals. All the results on bioluminescent SNP genotyping were confirmed by Sanger sequencing. Some features characteristic of the population were found, i.e. melanoma is mostly associated with R160W or R151C while variant D294H is extremely rare; simultaneous carriage of any two investigated variants is also strongly associated with melanoma; R151C is associated with ulceration and consequently the disease course is more aggressive, etc. The design of the technique allows fast evaluation of any known diagnostically important SNP frequencies and associations across population. © 2018 Elsevier B.V.

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Держатели документа:
Siberian Federal University, Svobodny pr. 79, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok 50/50, Krasnoyarsk, Russian Federation
Blokhin Cancer Research Center, Moscow, Russian Academy of Medical Sciences, Kashirskoye Shosse 24, Moscow, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, SB RAS, Novosibirsk Lavrentiev Avenue 8, Novosibirsk, Russian Federation
State Medical University named after V.F. Voyno-Yasenetsky, Partizana Zheleznyaka St. 1, Krasnoyarsk, Russian Federation
Regional Clinical Oncology Center named after A.I. Kryzhanovsky, 1 Smolenskaya Str.16, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Bashmakova, E. E.; Krasitskaya, V. V.; Bondar, A. A.; Eremina, E. N.; Slepov, E. V.; Zukov, R. A.; Frank, L. A.

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9.


   
    Developing the technology of physicochemical processing of organic waste in closed life support systems for space applications / A. A. Tikhomirov, S. V. Trifonov, E. A. Morozov // IOP Conference Series: Materials Science and Engineering : Institute of Physics Publishing, 2018. - Vol. 450: 9th International Multidisciplinary Scientific and Research Conference on Modern Issues in Science and Technology Workshop in Advanced Technologies in Aerospace, Mechanical and Automation Engineering, MISTAerospace 2018 (20 October 2018 through 28 October 2018, ) Conference code: 143027, Is. 6, DOI 10.1088/1757-899X/450/6/062017
Кл.слова (ненормированные):
Electric fields -- Environmental management -- Fighter aircraft -- Life support systems (spacecraft) -- Mass transfer -- Space applications -- Structural design -- Wastes -- Alternating current -- Combustion method -- Individual components -- Mineral nutrients -- Optimal parameter -- Physicochemical methods -- Technical implementation -- Waste processing -- Waste incineration
Аннотация: Closed life support systems for space applications need a technology of processing organic waste produced in the system that would enable incorporating the recycled waste into the mass transfer of the system. Researchers of the Institute of Biophysics SB RAS have developed a method of waste processing that meets these requirements: a physicochemical method of organic waste oxidation in the hydrogen peroxide aqueous solution under application of an alternating current electric field - wet combustion. The mineralized solution produced by this method can be used as a mineral nutrient supplement for higher plants in the life support system. The present study describes technical implementation of the wet combustion method and reports results of developing this method in the last few years. The study addresses problems associated with the design and positions of individual components and different configurations of the wet combustion reactor, showing the way to automate operation of the reactor and reporting optimal parameters of the current applied to the electrodes, which reduce time and power consumption by waste processing. © 2018 Institute of Physics Publishing. All rights reserved.

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center, Krasnoyarsk Science Center SB RAS, Krasnoyarsk, Akademgorodok, 660036, Russian Federation
Reshetnev Siberian State University of Science and Technology, 31 Krasnoyarskiy Rabochiy pr., Krasnoyarsk, 660037, Russian Federation

Доп.точки доступа:
Tikhomirov, A. A.; Trifonov, S. V.; Morozov, E. A.

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10.


   
    Enzymatic bioassay of soil: Sensitivity comparison of mono-, double- And triple-enzyme systems to soil toxicants / O. S. Sutormin [и др.] // Tsitologiya. - 2018. - Vol. 60, Is. 10. - С. 826-829, DOI 10.7868/S0041377118100132 . - ISSN 0041-3771
Кл.слова (ненормированные):
Bacterial luciferase -- Bioluminescent analysis -- Coupled enzyme systems -- Ecological monitoring -- Enzymatic toxicity bioassays -- Lactate dehydrogenase -- NADH:FMN-oxidoreductase -- Soil
Аннотация: In this paper, we have investigated the possibilities of application of enzymatic systems with increasing chain length as a bioassay to evaluate the soil contamination status. The sensitivity of monoenzyme reaction as well as double- and triple-enzyme chains based on NAD(P)H:FMN-oxidoreductase and luciferase of luminous bacteria and lactate dehydrogenase to pesticides and copper ions in water and water extracts from soils were estimated. For this, the toxicological parameter IC 20 reflecting the sensitivity limit of the enzyme system to the to-xicant was used. It was revealed that elongation of the coupled enzyme chain (from mono- to triple-enzyme) increases the sensitivity of the bioassay, in some cases by several orders of magnitude. This pattern can be used as a tool to improve the properties of enzymic bioassays. The effect of extracts from uncontaminated soils of various types on enzymatic systems also differs, which makes possible to design the specialized enzymatic bioassays as well. © 2018 Sankt Peterburg.All rights reserved.

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Держатели документа:
Siberian Federal University, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics Siberian Branch of RAS, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Sutormin, O. S.; Kolosova, E. M.; Nemtseva, A. V.; Iskorneva, I. V.; Lisitsa, A. A.; Matvienko, V. S.; Esimbekova, A. N.; Kratasyuk, V. A.

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11.


   
    Exploring Bioluminescence Function of the Ca2+-regulated Photoproteins with Site-directed Mutagenesis / E. V. Eremeeva, E. S. Vysotski // Photochem. Photobiol. - 2019. - Vol. 95, Is. 1. - P8-23, DOI 10.1111/php.12945. - Cited References:88. - This work was supported by grant 17-04-00764 of Russian Foundation for Basic Research and the state budgetallocated to the fundamental research at the Russian Academy of Sciences (project 0356-2017-0017). . - ISSN 0031-8655. - ISSN 1751-1097
РУБ Biochemistry & Molecular Biology + Biophysics
Рубрики:
CALCIUM-BINDING PHOTOPROTEIN
   GREEN-FLUORESCENT PROTEIN
   JELLYFISH
Кл.слова (ненормированные):
bioluminescence -- coelenterazine -- aequorin -- obelin -- clytin -- mitrocomin -- EF-hand protein
Аннотация: Site-directed mutagenesis is a powerful tool to investigate the structure-function relationship of proteins and a function of certain amino acid residues in catalytic conversion of substrates during enzymatic reactions. Hence, it is not surprising that this approach was repeatedly applied to elucidate the role of certain amino acid residues in various aspects of photoprotein bioluminescence, mostly for aequorin and obelin, and to design mutant photoproteins with altered properties (modified calcium affinity, faster or slower bioluminescence kinetics, different emission color) which would either allow the development of novel bioluminescent assays or improvement of characteristics of the already existing ones. This information, however, is scattered over different articles. In this review, we systematize the findings that were made using site-directed mutagenesis studies regarding the impact of various amino acid residues on bioluminescence of hydromedusan Ca2+-regulated photoproteins. All key residues that have been identified are pinpointed, and their influence on different aspects of photoprotein functioning such as active photoprotein complex formation, bioluminescence reaction, calcium response and light emitter formation is discussed.

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Держатели документа:
RAS, SB, Inst Biophys, Fed Res Ctr,Krasnoyarsk Sci Ctr,Photobiol Lab, Krasnoyarsk, Russia.

Доп.точки доступа:
Eremeeva, Elena V.; Vysotski, Eugene S.; Russian Foundation for Basic Research [17-04-00764]; Russian Academy of Sciences [0356-2017-0017]

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12.


   
    Global data set of long-term summertime vertical temperature profiles in 153 lakes / R. M. Pilla, E. M. Mette, C. E. Williamson [et al.] // Sci. Data. - 2021. - Vol. 8, Is. 1. - Ст. 200, DOI 10.1038/s41597-021-00983-y . - ISSN 2052-4463
Аннотация: Climate change and other anthropogenic stressors have led to long-term changes in the thermal structure, including surface temperatures, deepwater temperatures, and vertical thermal gradients, in many lakes around the world. Though many studies highlight warming of surface water temperatures in lakes worldwide, less is known about long-term trends in full vertical thermal structure and deepwater temperatures, which have been changing less consistently in both direction and magnitude. Here, we present a globally-expansive data set of summertime in-situ vertical temperature profiles from 153 lakes, with one time series beginning as early as 1894. We also compiled lake geographic, morphometric, and water quality variables that can influence vertical thermal structure through a variety of potential mechanisms in these lakes. These long-term time series of vertical temperature profiles and corresponding lake characteristics serve as valuable data to help understand changes and drivers of lake thermal structure in a time of rapid global and ecological change. © 2021, The Author(s).

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Держатели документа:
Miami University, Department of Biology, Oxford, OH, United States
Belarusian State University, Faculty of Biology, Minsk, Belarus
Leibniz-Institute of Freshwater Ecology and Inland Fisheries, Department of Ecosystem Research, Berlin, Germany
INRAE, University of Savoie Mont-Blanc, CARRTEL, Thonon-les-Bains, France
University of Comahue: INIBIOMA, CONICET, Neuquen, Argentina
University of Shiga Prefecture, Hikone, Shiga, Japan
University of Nevada, Reno, Global Water Center, Reno, NV, United States
Uppsala University, Department of Ecology and Genetics/Limnology, Uppsala, Sweden
University of Montana, Flathead Lake Biological Station, Polson, Montana, United States
Universidad del Valle de Guatemala Centro de Estudios Atitlan, Guatemala, Guatemala
University of Innsbruck, Research Department for Limnology Mondsee, Mondsee, Austria
Mohonk Preserve, Daniel Smiley Research Center, New Paltz, NY, United States
UK Centre for Ecology & Hydrology, Lake Ecosystems Group, Lancaster, United Kingdom
Seqwater, Ipswich, QLD, Australia
Florida International University, Department of Biological Sciences and Institute of Environment, Miami, FL, United States
U.S. National Park Service, Crater Lake National Park, Crater Lake, OR, United States
University of Oklahoma, Department of Biology, Norman, OK, United States
Griffith University, Australian Rivers Institute, Nathan, Australia
University of Florida, Gainesville, FL, United States
University of Oslo, Department of Biosciences, Oslo, Norway
LUBW Landesanstalt fur Umwelt, Messungen und Naturschutz Baden-Wurttemberg, Institut fur Seenforschung, Langenargen, Germany
IISD Experimental Lake Area Inc., Winnipeg, MB, Canada
FAO, BELSPO, Brussels, Belgium
University of Eastern Finland, Department of Environmental and Biological Sciences, Joensuu, Finland
Swiss Federal Institute of Aquatic Science and Technology, Department of Aquatic Ecology, Dubendorf, Switzerland
CSIRO, Land and Water, Canberra, Australia
Laurentian University, Cooperative Freshwater Ecology Unit, Sudbury, Ontario, Canada
Fairfield University, Biology Department, Fairfield, CT, United States
University of Minnesota, Itasca Biological Station and Laboratories, Lake Itasca, MN, United States
Finnish Environment Institute SYKE, Freshwater Center, Helsinki, Finland
A.N. Severtsov Institute of Ecology and Evolution of The Russian Academy of Sciences, Laboratory of Ecology of Water Communities and Invasions, Moscow, Russian Federation
Zurich Water Supply, City of Zurich, Zurich, Switzerland
University of Regina, Institute of Environmental Change and Society, Regina, SK, Canada
Milano-Bicocca University, Milan, Italy
University of Applied Sciences and Arts of Southern Switzerland, Department for Environment, Constructions and Design, Canobbio, Switzerland
Kamchatka Research Institute of Fisheries & Oceanography, now Kamchatka Branch of Russian Federal Research Institute of Fisheries and Oceanography, Petropavlovsk-Kamchatsky, Russian Federation
University of Wisconsin, Center for Limnology, Boulder Junction, WI, United States
Federal Agency for Water Management, Institute for Aquatic Ecology and Fisheries Management, Mondsee, Austria
University of California Santa Barbara, Department of Ecology, Evolution and Marine Biology, Santa Barbara, California, United States
University of Waikato, Environmental Research Institute, Hamilton, New Zealand
Ryerson University, Department of Chemistry and Biology, Toronto, ON, Canada
University of Hamburg, Department of Biology, Hamburg, Germany
Dominion Diamond Mines, Environment Department, Calgary, AB, Canada
Ontario Ministry of the Environment, Conservation and Parks, Dorset Environmental Science Centre, Dorset, ON, Canada
Irkutsk State University, Institute of Biology, Irkutsk, Russian Federation
University of Liege, Chemical Oceanography Unit, Institut de Physique (B5A), Liege, Belgium
SUNY New Paltz, Biology Department, New Paltz, NY, United States
The Kinneret Limnological Laboratory, Israel Oceanographic and Limnological Research, Migdal, Israel
CNR Water Research institute, Verbania, Verbania, Pallanza, Italy
Krasnoyarsk Scientific Center SB RAS, Institute of Biophysics, Krasnoyarsk, Russian Federation
University of California Davis, Department of Environmental Science and Policy, Davis, CA, United States
Fondazione Edmund Mach, Research and Innovation Centre, San Michele all’Adige, Italy
University of Maine, Climate Change Institute, Orono, ME, United States
University of Turku, Turku, Finland
Universite Laval, Departments of Biology and Geography, Quebec, Canada
University of Washington, School of Aquatic and Fishery Sciences, Seattle, WA, United States
The Technical University of Kenya, Department of Geosciences and the Environment, Nairobi, Kenya
University of Innsbruck, Department of Ecology, Innsbruck, Austria
University of Konstanz, Limnological Institute, Konstanz, Germany
Dickinson College, Department of Environmental Science, Carlisle, PA, United States
Archbold Biological Station, Venus, FL, United States
University of Michigan, Biological Station, Pellston, MI, United States
Vrije Universiteit Brussel, Department of Hydrology and Hydraulic Engineering, Brussels, Belgium
ETH Zurich, Institute for Atmospheric and Climate Science, Zurich, Switzerland
National Institute of Water & Atmospheric Research, Hamilton, New Zealand
University of Alberta, Department of Biological Sciences, Edmonton, AB, Canada
Cary Institute of Ecosystem Studies, Millbrook, NY, United States

Доп.точки доступа:
Pilla, R. M.; Mette, E. M.; Williamson, C. E.; Adamovich, B. V.; Adrian, R.; Anneville, O.; Balseiro, E.; Ban, S.; Chandra, S.; Colom-Montero, W.; Devlin, S. P.; Dix, M. A.; Dokulil, M. T.; Feldsine, N. A.; Feuchtmayr, H.; Fogarty, N. K.; Gaiser, E. E.; Girdner, S. F.; Gonzalez, M. J.; Hambright, K. D.; Hamilton, D. P.; Havens, K.; Hessen, D. O.; Hetzenauer, H.; Higgins, S. N.; Huttula, T. H.; Huuskonen, H.; Isles, P. D.F.; Joehnk, K. D.; Keller, W. B.; Klug, J.; Knoll, L. B.; Korhonen, J.; Korovchinsky, N. M.; Koster, O.; Kraemer, B. M.; Leavitt, P. R.; Leoni, B.; Lepori, F.; Lepskaya, E. V.; Lottig, N. R.; Luger, M. S.; Maberly, S. C.; MacIntyre, S.; McBride, C.; McIntyre, P.; Melles, S. J.; Modenutti, B.; Muller-Navarra, D. C.; Pacholski, L.; Paterson, A. M.; Pierson, D. C.; Pislegina, H. V.; Plisnier, P. -D.; Richardson, D. C.; Rimmer, A.; Rogora, M.; Rogozin, D. Y.; Rusak, J. A.; Rusanovskaya, O. O.; Sadro, S.; Salmaso, N.; Saros, J. E.; Sarvala, J.; Saulnier-Talbot, E.; Schindler, D. E.; Shimaraeva, S. V.; Silow, E. A.; Sitoki, L. M.; Sommaruga, R.; Straile, D.; Strock, K. E.; Swain, H.; Tallant, J. M.; Thiery, W.; Timofeyev, M. A.; Tolomeev, A. P.; Tominaga, K.; Vanni, M. J.; Verburg, P.; Vinebrooke, R. D.; Wanzenbock, J.; Weathers, K.; Weyhenmeyer, G. A.; Zadereev, E. S.; Zhukova, T. V.

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13.


   
    Design of bioluminescent biosensors for assessing contamination of complex matrices / E. N. Esimbekova, V. P. Kalyabina, K. V. Kopylova [et al.] // Talanta. - 2021. - Vol. 233. - Ст. 122509, DOI 10.1016/j.talanta.2021.122509. - Cited References:87. - The reported study was funded by Russian Foundation for Basic Research, Government of Krasnoyarsk Territory, Krasnoyarsk Regional Fund of Science, to the research project No. 20-44-242001 and Ministry of Science and Higher Education of Russian Federation No. FSRZ-2020-0006. . - ISSN 0039-9140. - ISSN 1873-3573
РУБ Chemistry, Analytical
Рубрики:
SAMPLE PREPARATION
   PESTICIDES
   FOOD
   BIOMOLECULES
   SENSITIVITY
Кл.слова (ненормированные):
Bioluminescent biosensor -- Enzyme inhibition-based assay -- Complex -- matrices -- Pesticides -- Heavy metals
Аннотация: The presence of potentially toxic xenobiotics in complex matrices has become rather the rule than the exception. Therefore, there is a need for highly sensitive inexpensive techniques for analyzing environmental and food matrices for toxicants. Enzymes are selectively sensitive to various toxic compounds, and, thus, they can be used as the basis for detection of contaminants in complex matrices. There are, however, a number of difficulties associated with the analysis of complex matrices using enzyme assays, including the necessity to take into account properties and effects of the natural components of the test media for accurate interpretation of results. The present study describes the six-stage procedure for designing new enzyme sensors intended for assessing the quality of complex matrices. This procedure should be followed both to achieve the highest possible sensitivity of the biosensor to potentially toxic substances and to minimize the effect of the uncontaminated components of complex mixtures on the activity of the biosensor. The proposed strategy has been tested in designing a bioluminescent biosensor for integrated rapid assessment of the safety of fruits and vegetables. The biosensor is based on the coupled enzyme system NAD(P)H:FMN-oxidoreductase and luciferase as the biorecognition element. The study describes methods and techniques for attaining the desired result in each stage. The proposed six-stage procedure for designing bioluminescent enzyme biosensors can be used to design the enzymatic biosensors based on other enzymes.

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Держатели документа:
Siberian Fed Univ, 79 Svobodny Prospect, Krasnoyarsk 660041, Russia.
Inst Biophys SB RAS, 50-50 Akademgorodok, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Esimbekova, Elena N.; Kalyabina, Valeriya P.; Kopylova, Kseniya, V; Torgashina, Irina G.; Kratasyuk, Valentina A.; Kopylova, Kseniya; Esimbekova, Elena; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR); Government of Krasnoyarsk Territory; Krasnoyarsk Regional Fund of Science [20-44-242001]; Ministry of Science and Higher Education of Russian Federation [FSRZ-2020-0006]

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14.


   
    Global data set of long-term summertime vertical temperature profiles in 153 lakes / R. M. Pilla, E. M. Mette, C. E. Williamson [et al.] // Sci. Data. - 2021. - Vol. 8, Is. 1. - Ст. 200, DOI 10.1038/s41597-021-00983-y. - Cited References:45. - This work was conceived at the Global Lake Ecological Observatory Network (GLEON), and benefited from continued participation and travel support from GLEON. This manuscript is dedicated to the late Karl Havens and Alon Rimmer, who provided data for this manuscript. Funding and support for this work came from the following sources: the Belarus Republican Foundation for Fundamental Research; the IGB Long-term Ecological Research Programme; SOERE OLA, AnaEE-France, INRA Thonon les Bains, SILA (Syndicat Mixte du Lac d'Annecy), CISALB (Comite Intercommunautaire pour l'Assainissement du Lac du Bourget), and CIPEL (Commission Internationale pour la protection des eaux du Leman); Shiga Prefectural Fisheries Experiment Station (SPFES); Castle Lake Environmental Research and Education Program, University of Nevada at Reno and UC Davis; the Flathead Lake Monitoring program funded through a consortium of state and private funds, and thank the generous citizens of Flathead Lake for their continued support of lake monitoring; the Institute for water ecology, fish biology and lake research and the Institute for Limnology of the Austrian Academy of Sciences (until 2011), and acknowledge the sampling efforts by many individuals over the long period of investigation, especially H. Gassner, M. Luger, H. Ficker, and R. Kurmayer; the EC project "Response of European Freshwater Lakes to Environmental and Climatic Change" (REFLECT, ENV4-CT97-0453), the EC-project "Climate Impacts on European Lakes" (CLIME, EVK1-CT-2002-00121), the project "Risk Analysis of Direct and Indirect Climate effects on deep Austrian Lake Ecosystems" (RADICAL) funded by the Austrian Climate and Energy Fund (No. K09ACK00046) -Austrian Climate Research Programme (ACRP, http://www.klimafonds.gv.at); O. Garcia and E. Bocel for data analysis and management; D. Cabrera, M.W. Dix, G. Ochaeta, S. van Tuylen, M. Orozco, E. Symonds for sampling efforts; NSF grant No. 0947096 to E. Rejmankova, U.S. PeaceCorps and Ministerio de Ambiente y Recursos Naturales of Guatemala; H. Swain, L. Battoe, K. Main, N. Deyrup (Archbold Biological Station), the Florida Lakewatch program, E. Gaiser (Florida International University); the Crater Lake National Park Long-Term Limnological Monitoring Program; the City of Tulsa (R. West and A. Johnson), the Grand River Dam Authority (R. M. Zamor), W.M. Matthews and US ACE (T. Clyde), and the Oklahoma Water Resources Board; Bay of Plenty Regional Council; Ministry of Business, Innovation and Employment: Enhancing the Health and Resilience of New Zealand lakes (UOWX1503); the field and laboratory staff of the South Florida Water Management District for collecting and analyzing the samples; the Norwegian Water Resources and Energy Directorate (NVE), by courtesy of A. S. Kvambekk; the Lake Champlain Long-term Monitoring program (VT DEC and NY DEC); the National Capital Authority, ACT, Australia; Ontario Ministry of Environment, Conservation and Parks; FirstLight Power Resources and Friends of the Lake, especially G. Bollard and R. White; the Finnish Environment Institute SYKE database (Hertta) and S. Mitikka; N. Spinelli and the Lake Wallenpaupack Watershed Management District; Lakes Heywood, Moss, and Sombre: Long-Term Monitoring of Signy Lake Chemistry by BAS 1963-2004. Ref: GB/NERC/BAS/AEDC/00063, and dataset supplied by the Polar Data Centre under Open Government License (c) NERC-BAS, Lake Nkugute: Beadle (1966), CLANIMAE project funded by the Belgian Science Policy Office; Dr. L.; Garibaldi; NSF awards #1418698 and North Temperate Lakes LTER NTL-LTER #1440297; NSERC Canada, Canada Research Chairs, Canada Foundation for Innovation, Province of Saskatchewan, University of Regina, and Queen's University Belfast; Commissione Internazionale per la protezione delle acque italo-svizzere, Ufficio della protezione delle acque e dell'approvvigionamento idrico del Canton Ticino; KamchatNIRO scientists; Natural Environment Research Council award number NE/R016429/1 as part of the UK-SCaPE programme delivering National Capability; U.S. NSF Arctic LTER DEB1637459; Belgian Science Policy (Choltic, Climlake, Climfish); Ontario Ministry of Natural Resources' Harkness Laboratory of Fisheries Research, especially T. Middel; Max-Planck-Institute for Limnology Plon; staff at Erken Laboratory; Mohonk Preserve and D. Smiley; Lake Sunapee Protective Association; KLL database; International Commission for the Protection of Swiss-Italian Waters (CIPAIS) and the LTER (Long Term Ecological Research) Italian network, site "Southern Alpine lakes", LTER_EU_IT_008; staff and students at MECP's Dorset Environmental Science Centre; the LTER (Long-Term Ecological Research) Italian network, site "Southern Alpine lakes", IT08-005-A (http://www.lteritalia.it), with the support of the ARPA Veneto; Prof. L. Chapman, McGill University (Montreal, Quebec, Canada); Amt fur Abfall, Wasser, Energie und Luft (AWEL) of the Canton of Zurich; grants of RSCF project #18-44-06201 and #20-64-46003, of Russian Ministry of Higher Education and Research (projects. FZZE-2020-0026;. FZZE-2020-0023), and of Foundation for support of applied ecological studies "Lake Baikal" (https://baikalfoundation.ru/project/tochka-1/); National Science Foundation Long Term Research in Environmental Biology program (DEB-1242626); the National Park Service (the Inventory and Monitoring Program as well as the Air Resources Division) and Acadia National Park and the Acadia National Park monitoring program; Gordon and Betty Moore Foundation, the Andrew Mellon Foundation, the US National Science Foundation and the Bristol Bay salmon processors; J. Franzoi, G. Larsen, and S. Morales, and the LTSER platform Tyrolean Alps, which belongs to the national and international long-term ecological research network (LTER-Austria, LTER Europe and ILTER); Institut fur Seenforschung, Langenargen (Internationale Gewasserschutzkommission fur den Bodensee -IGKB); University of Michigan Biological Station (A. Schubel) and Cooperative Institute for Great Lakes Research (R. Miller); the Belgian Science Policy Office (BELSPO) is acknowledged for supporting research on Lake Kivu through the research project EAGLES (CD/AR/02 A); US National Science Foundation awards 9318452, 9726877, 0235755, 0743192 and 1255159; West Coast Regional Council, the Bay of Plenty Regional Council, and Waikato Regional Council, and NIWA; D. Schindler (funding and data access) and B. Parker (logistical support and data management); Swedish Infrastructure for Ecosystem Science (SITES) and the Swedish Research Council under the grant no 2017-00635; NSF DEB 1754276 and NSF DEB 1950170, the Ohio Eminent Scholar in Ecosystem Ecology fund, and Lacawac Sanctuary and Biological Field Station; Russian Foundation for Basic Research, grant. 19-04-00362 A and. 19-05-00428. . - ISSN 2052-4463
РУБ Multidisciplinary Sciences
Рубрики:
CLIMATE-CHANGE
   THERMAL STRATIFICATION
   OXYGEN DEPLETION
   FISH
Аннотация: Climate change and other anthropogenic stressors have led to long-term changes in the thermal structure, including surface temperatures, deepwater temperatures, and vertical thermal gradients, in many lakes around the world. Though many studies highlight warming of surface water temperatures in lakes worldwide, less is known about long-term trends in full vertical thermal structure and deepwater temperatures, which have been changing less consistently in both direction and magnitude. Here, we present a globally-expansive data set of summertime in-situ vertical temperature profiles from 153 lakes, with one time series beginning as early as 1894. We also compiled lake geographic, morphometric, and water quality variables that can influence vertical thermal structure through a variety of potential mechanisms in these lakes. These long-term time series of vertical temperature profiles and corresponding lake characteristics serve as valuable data to help understand changes and drivers of lake thermal structure in a time of rapid global and ecological change.

WOS
Держатели документа:
Miami Univ, Dept Biol, Oxford, OH 45056 USA.
Belarusian State Univ, Fac Biol, Minsk, BELARUS.
Leibniz Inst Freshwater Ecol & Inland Fisheries, Dept Ecosyst Res, Berlin, Germany.
Univ Savoie Mont Blanc, INRAE, CARRTEL, Thonon Les Bains, France.
Univ Comahue INIBIOMA, CONICET, Neuquen, Argentina.
Univ Shiga Prefecture, Shiga, Japan.
Univ Nevada, Global Water Ctr, Reno, NV 89557 USA.
Uppsala Univ, Dept Ecol & Genet Limnol, Uppsala, Sweden.
Univ Montana, Flathead Lake Biol Stn, Polson, MT 59860 USA.
Univ Valle Guatemala, Ctr Estudios Atitlan, Guatemala City, Guatemala.
Univ Innsbruck, Res Dept Limnol Mondsee, Mondsee, Austria.
Daniel Smiley Res Ctr, Mohonk Preserve, New Paltz, NY USA.
Lake Ecosyst Grp, UK Ctr Ecol & Hydrol, Lancaster, England.
Seqwater, Ipswich, Qld, Australia.
Florida Int Univ, Dept Biol Sci, Miami, FL 33199 USA.
Inst Environm, Miami, FL USA.
Natl Pk Serv, Crater Lake Natl Pk, Crater Lake, OR USA.
Univ Oklahoma, Dept Biol, Norman, OK 73019 USA.
Griffith Univ, Australian Rivers Inst, Nathan, Qld, Australia.
Univ Florida, Gainesville, FL USA.
Univ Oslo, Dept Biosci, Oslo, Norway.
Inst Seenforschung, LUBW Landesanstalt Umwelt Messungen & Naturschutz, Langenargen, Germany.
IISD Expt Lake Area Inc, Winnipeg, MB, Canada.
BELSPO, FAO, Brussels, Belgium.
Univ Eastern Finland, Dept Environm & Biol Sci, Joensuu, Finland.
Swiss Fed Inst Aquat Sci & Technol, Dept Aquat Ecol, Dubendorf, Switzerland.
CSIRO, Land & Water, Canberra, ACT, Australia.
Laurentian Univ, Cooperat Freshwater Ecol Unit, Sudbury, ON, Canada.
Fairfield Univ, Dept Biol, Fairfield, CT 06430 USA.
Univ Minnesota, Itasca Biol Stn & Labs, Lake Itasca, MN USA.
Finnish Environm Inst SYKE, Freshwater Ctr, Helsinki, Finland.
Russian Acad Sci, Lab Ecol Water Communities & Invas, AN Severtsov Inst Ecol & Evolut, Moscow, Russia.
Zurich Water Supply, Zurich, Switzerland.
Univ Regina, Inst Environm Change & Soc, Regina, SK, Canada.
Milano Bicocca Univ, Milan, Italy.
Univ Appl Sci & Arts Southern Switzerland, Dept Environm Construct & Design, Canobbio, Switzerland.
Russian Fed Res Inst Fisheries & Oceanog, Kamchatka Res Inst Fisheries & Oceanog, Kamchatka Branch, Petropavlovsk Kamchatski, Russia.
Univ Wisconsin, Ctr Limnol, Boulder Jct, WI USA.
Inst Aquat Ecol & Fisheries Management, Fed Agcy Water Management, Mondsee, Austria.
Univ Calif Santa Barbara, Dept Ecol Evolut & Marine Biol, Santa Barbara, CA 93106 USA.
Univ Waikato, Environm Res Inst, Hamilton, New Zealand.
Ryerson Univ, Dept Biol & Chem, Toronto, ON, Canada.
Univ Hamburg, Dept Biol, Hamburg, Germany.
Dominion Diamond Mines, Environm Dept, Calgary, AB, Canada.
Ontario Minist Environm Conservat & Pk, Dorset Environm Sci Ctr, Dorset, ON, Canada.
Irkutsk State Univ, Inst Biol, Irkutsk, Russia.
Univ Liege, Inst Phys B5A, Chem Oceanog Unit, Liege, Belgium.
SUNY Coll New Paltz, Dept Biol, New Paltz, NY USA.
Israel Oceanog & Limnol Res, Kinneret Limnol Lab, Migdal, Israel.
CNR Water Res Inst, Verbania, Pallanza, Italy.
RAS, Inst Biophys, Krasnoyarsk Sci Ctr, SB, Krasnoyarsk, Russia.
Univ Calif Davis, Dept Environm Sci & Policy, Davis, CA 95616 USA.
Fdn Edmund Mach, Res & Innovat Ctr, San Michele All Adige, Italy.
Univ Maine, Climate Change Inst, Orono, ME USA.
Univ Turku, Turku, Finland.
Univ Laval, Dept Biol, Quebec City, PQ, Canada.
Univ Laval, Dept Geog, Quebec City, PQ, Canada.
Univ Washington, Sch Aquat & Fishery Sci, Seattle, WA 98195 USA.
Tech Univ Kenya, Dept Geosci & Environm, Nairobi, Kenya.
Univ Innsbruck, Dept Ecol, Innsbruck, Austria.
Univ Konstanz, Limnol Inst, Constance, Germany.
Dickinson Coll, Dept Environm Sci, Carlisle, PA 17013 USA.
Archbold Biol Stn, Venus, FL USA.
Univ Michigan, Biol Stn, Pellston, MI USA.
Vrije Univ Brussel, Dept Hydrol & Hydraul Engn, Brussels, Belgium.
Swiss Fed Inst Technol, Inst Atmospher & Climate Sci, Zurich, Switzerland.
Natl Inst Water & Atmospher Res, Hamilton, New Zealand.
Univ Alberta, Dept Biol Sci, Edmonton, AB, Canada.
Cary Inst Ecosyst Studies, Millbrook, NY USA.

Доп.точки доступа:
Pilla, Rachel M.; Mette, Elizabeth M.; Williamson, Craig E.; Adamovich, Boris V.; Adrian, Rita; Anneville, Orlane; Balseiro, Esteban; Ban, Syuhei; Chandra, Sudeep; Colom-Montero, William; Devlin, Shawn P.; Dix, Margaret A.; Dokulil, Martin T.; Feldsine, Natalie A.; Feuchtmayr, Heidrun; Fogarty, Natalie K.; Gaiser, Evelyn E.; Girdner, Scott F.; Gonzalez, Maria J.; Hambright, K. David; Hamilton, David P.; Havens, Karl; Hessen, Dag O.; Hetzenauer, Harald; Higgins, Scott N.; Huttula, Timo H.; Huuskonen, Hannu; Isles, Peter D. F.; Joehnk, Klaus D.; Keller, Wendel Bill; Klug, Jen; Knoll, Lesley B.; Korhonen, Johanna; Korovchinsky, Nikolai M.; Koster, Oliver; Kraemer, Benjamin M.; Leavitt, Peter R.; Leoni, Barbara; Lepori, Fabio; Lepskaya, Ekaterina V.; Lottig, Noah R.; Luger, Martin S.; Maberly, Stephen C.; MacIntyre, Sally; McBride, Chris; McIntyre, Peter; Melles, Stephanie J.; Modenutti, Beatriz; Muller-Navarra, L.; Pacholski, Laura; Paterson, Andrew M.; Pierson, Don C.; Pislegina, Helen V.; Plisnier, Pierre-Denis; Richardson, David C.; Rimmer, Alon; Rogora, Michela; Rogozin, Denis Y.; Rusak, James A.; Rusanovskaya, Olga O.; Sadro, Steve; Salmaso, Nico; Saros, Jasmine E.; Sarvala, Jouko; Saulnier-Talbot, Emilie; Schindler, Daniel E.; Shimaraeva, Svetlana V.; Silow, Eugene A.; Sitoki, Lewis M.; Sommaruga, Ruben; Straile, Dietmar; Strock, Kristin E.; Swain, Hilary; Tallant, Jason M.; Thiery, Wim; Timofeyev, Maxim A.; Tolomeev, Alexander P.; Tominaga, Koji; Vanni, Michael J.; Verburg, Piet; Vinebrooke, Rolf D.; Wanzenbock, Josef; Weathers, Kathleen; Weyhenmeyer, Gesa A.; Zadereev, Egor S.; Zhukova, Tatyana V.; Johnk, Klaus; Belarus Republican Foundation for Fundamental Research; AnaEE-France; SILA (Syndicat Mixte du Lac d'Annecy); Castle Lake Environmental Research and Education Program, University of Nevada at Reno; EC project "Response of European Freshwater Lakes [ENV4-CT97-0453]; EC-project "Climate Impacts on European Lakes" [EVK1-CT-2002-00121]; Austrian Climate and Energy Fund [K09ACK00046]; NSFNational Science Foundation (NSF) [DEB 1950170]; Crater Lake National Park Long-Term Limnological Monitoring Program; Ministry of Business, Innovation and Employment: Enhancing the Health and Resilience of New Zealand lakes [UOWX1503]; National Capital Authority; ACT, Australia [GB/NERC/BAS/AEDC/00063]; Belgian Science Policy OfficeBelgian Federal Science Policy Office; North Temperate Lakes LTER NTL-LTER [1440297]; NSERC CanadaNatural Sciences and Engineering Research Council of Canada (NSERC); Canada Research Chairs, Canada Foundation for InnovationCanada Foundation for InnovationCanada Research Chairs; University of Regina; Commissione Internazionale per la protezione delle acque italo-svizzere; Natural Environment Research CouncilUK Research & Innovation (UKRI)Natural Environment Research Council (NERC) [NE/R016429/1]; U.S. NSF Arctic LTER [DEB1637459, LTER_EU_IT_008]; Canton of Zurich [18-44-06201, 20-64-46003]; Russian Ministry of Higher Education and Research [FZZE-2020-0026, FZZE-2020-0023]; National Science Foundation Long Term Research in Environmental Biology program [DEB-1242626]; National Park Service (the Inventory and Monitoring Program); Acadia National Park monitoring program; Gordon and Betty Moore FoundationGordon and Betty Moore Foundation; Andrew Mellon Foundation; US National Science FoundationNational Science Foundation (NSF) [9318452, 9726877, 0235755, 0743192, 1255159]; Institut fur Seenforschung, Langenargen (Internationale Gewasserschutzkommission fur den Bodensee -IGKB); University of Michigan Biological StationUniversity of Michigan System; Belgian Science Policy Office (BELSPO)Belgian Federal Science Policy Office [CD/AR/02 A]; Waikato Regional Council; NIWA; Swedish Research CouncilSwedish Research CouncilEuropean Commission [2017-00635, NSF DEB 1754276]; Lacawac Sanctuary and Biological Field Station; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-04-00362 A, 19-05-00428]

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15.


   
    Modeling a lunar base mushroom farm / V. S. Kovalev, W. Grandl, N. S. Manukovsky [et al.] // Life Sci. Space Res. - 2022. - Vol. 33. - P1-6, DOI 10.1016/j.lssr.2021.12.005. - Cited References:31 . - ISSN 2214-5524. - ISSN 2214-5532
РУБ Astronomy & Astrophysics + Biology + Multidisciplinary Sciences
Рубрики:
SUPPORT
   CULTIVATION
Кл.слова (ненормированные):
Mushroom -- Module -- Structure -- Design -- Calculation
Аннотация: To calculate the equivalent system mass of mushrooms, a conceptual configuration of a mushroom farm as part of a bioregenerative life support system on an inhabited lunar base was designed. The mushroom farm consists of two connected modules. Each module is a double-shell rigid pipe-in-pipe aluminum structure. The first module is used to prepare and sterilize the substrate, while the mushrooms are sown and grown in the second module. Planned productivity of the mushroom farm is 28 kg of fresh mushrooms per one process cycle lasting 66 days for 14 consumers. Mushroom production can be increased using additional modules. The calculated equivalent system masses of the mushroom farm and the mushrooms produced therein is 88,432 kg and 31,550 kg per 1 kg of dry mushrooms in one process cycle, respectively. At that, the biggest contributor to the equivalent system mass of mushrooms is the total pressurized volume of the farm - 68%. The results obtained may be a prerequisite for performing trade-off studies between different configurations of mushroom farm and calculating a space diet using the equivalent system mass of mushrooms.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Moscow, Russia.
Reshetnev Siberian State Univ Sci & Technol, Krasnoyarsk, Russia.

Доп.точки доступа:
Kovalev, V. S.; Grandl, W.; Manukovsky, N. S.; Tikhomirov, A. A.; Bock, C.

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16.


   
    Molecular insights into ligand recognition and G protein coupling of the neuromodulatory orphan receptor GPR139 / Y. L. Zhou, H. Daver, B. Trapkov [et al.] // Cell Res. - 2021, DOI 10.1038/s41422-021-00591-w. - Cited References:16. - This work was supported by the CAS Strategic Priority Research Program XDB37030104 (Z.-J.L.), the National Science Fund for Distinguished Young Scholars 32022038 (T.H.), the National Natural Science Foundation of China grants 31930060 (Z.-J.L.) and 31870744 (T.H.), and the Shanghai Rising-Star Program 20QA1406500 (T. H.), the Lundbeck Foundation R163-2013-16327 (D.E.G.), the Novo Nordisk Foundation NNF18OC0031226 (D.E.G.) and Independent Research Fund Denmark | Natural Sciences 8021-00173B (D.E.G.), the Lundbeck Foundation R355-2020-949 (B.T.) and the Carlsberg Foundation CF20-0248 (H.B.-O.). D.E.G. is a member of the Integrative Structural Biology at the University of Copenhagen (ISBUC). The cryo-EM data were collected at the Bio-Electron Microscopy Facility, ShanghaiTech University, with the assistance of Q.-Q. Sun, D.-D. Liu, Z.-H. Zhang and Y.-H. Liu. We thank the Assay Core, the assistance of F.-F. Zhou and Q.-W. Tan and the Cell Expression, Cloning and Purification Core Facilities of iHuman Institute for their support. . - Article in press. - ISSN 1001-0602. - ISSN 1748-7838
РУБ Cell Biology
Рубрики:
DISCOVERY
   PEPTIDES
   ALPHA

WOS
Держатели документа:
ShanghaiTech Univ, iHuman Inst, Shanghai, Peoples R China.
ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China.
Univ Chinese Acad Sci, Beijing, Peoples R China.
Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, CAS Ctr Excellence Mol Cell Sci, Shanghai, Peoples R China.
Univ Copenhagen, Dept Drug Design & Pharmacol, Univ Pk 2, Copenhagen, Denmark.
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Photobiol Lab, Akad Gorodok 50-50, Krasnoyarsk, Russia.

Доп.точки доступа:
Zhou, Yali; Daver, Henrik; Trapkov, Boris; Wu, Lijie; Wu, Meng; Harpsoe, Kasper; Gentry, Patrick R.; Liu, Kaiwen; Larionova, Marina; Liu, Junlin; Chen, N.a.; Brauner-Osborne, Hans; Gloriam, David E.; Hua, Tian; Liu, Zhi-Jie; CAS Strategic Priority Research Program [XDB37030104]; National Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars [32022038]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930060, 31870744]; Shanghai Rising-Star Program [20QA1406500]; Lundbeck FoundationLundbeckfonden [R355-2020-949, R163-2013-16327]; Novo Nordisk FoundationNovo Nordisk FoundationNovocure Limited [NNF18OC0031226]; Independent Research Fund Denmark | Natural Sciences [8021-00173B]; Carlsberg FoundationCarlsberg Foundation [CF20-0248]

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17.


   
    Design of bioluminescent biosensors for assessing contamination of complex matrices / E. N. Esimbekova, V. P. Kalyabina, K. V. Kopylova [et al.] // Talanta. - 2021. - Vol. 233. - Ст. 122509, DOI 10.1016/j.talanta.2021.122509. - Cited By :1 . - ISSN 0039-9140
Кл.слова (ненормированные):
Bioluminescent biosensor -- Complex matrices -- Enzyme inhibition-based assay -- Heavy metals -- Pesticides
Аннотация: The presence of potentially toxic xenobiotics in complex matrices has become rather the rule than the exception. Therefore, there is a need for highly sensitive inexpensive techniques for analyzing environmental and food matrices for toxicants. Enzymes are selectively sensitive to various toxic compounds, and, thus, they can be used as the basis for detection of contaminants in complex matrices. There are, however, a number of difficulties associated with the analysis of complex matrices using enzyme assays, including the necessity to take into account properties and effects of the natural components of the test media for accurate interpretation of results. The present study describes the six-stage procedure for designing new enzyme sensors intended for assessing the quality of complex matrices. This procedure should be followed both to achieve the highest possible sensitivity of the biosensor to potentially toxic substances and to minimize the effect of the uncontaminated components of complex mixtures on the activity of the biosensor. The proposed strategy has been tested in designing a bioluminescent biosensor for integrated rapid assessment of the safety of fruits and vegetables. The biosensor is based on the coupled enzyme system NAD(P)H:FMN-oxidoreductase and luciferase as the biorecognition element. The study describes methods and techniques for attaining the desired result in each stage. The proposed six-stage procedure for designing bioluminescent enzyme biosensors can be used to design the enzymatic biosensors based on other enzymes. © 2021 Elsevier B.V.

Scopus
Держатели документа:
Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Esimbekova, E. N.; Kalyabina, V. P.; Kopylova, K. V.; Torgashina, I. G.; Kratasyuk, V. A.

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18.


   
    Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi / K. A. Beregovaya, N. M. Myshkina, T. V. Chepurnykh [et al.] // Dokl. Biochem. Biophys. - 2021. - Vol. 496, Is. 1. - P14-17, DOI 10.1134/S1607672921010026. - Cited References:12. - This work was supported by the grant from the Russian Science Foundation no. 16-14-00052P, alanine screening was performed by the President grant for leading scientific schools NSh-2605.2020.4. . - ISSN 1607-6729. - ISSN 1608-3091
РУБ Biochemistry & Molecular Biology + Biophysics

Кл.слова (ненормированные):
bioluminescence -- luciferase -- Neonothopanus nambi -- rational design
Аннотация: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination.

WOS
Держатели документа:
Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow, Russia.
Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Fed Res Ctr, Krasnoyarsk, Russia.

Доп.точки доступа:
Beregovaya, K. A.; Myshkina, N. M.; Chepurnykh, T., V; Kotlobay, A. A.; Purtov, K., V; Petushkov, V. N.; Rodionova, N. S.; Yampolsky, I., V; Russian Science FoundationRussian Science Foundation (RSF) [16-14-00052P]; President grant for leading scientific schoolsLeading Scientific Schools Program [NSh-2605.2020.4]

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19.


   
    Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi / K. A. Beregovaya, N. M. Myshkina, T. V. Chepurnykh [et al.] // Doklad. Biochem. Biophys. - 2021. - Vol. 496, Is. 1. - P14-17, DOI 10.1134/S1607672921010026 . - ISSN 1607-6729
Кл.слова (ненормированные):
bioluminescence -- luciferase -- Neonothopanus nambi -- rational design
Аннотация: Abstract: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination. © 2021, Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Moscow, Russian Federation
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Beregovaya, K. A.; Myshkina, N. M.; Chepurnykh, T. V.; Kotlobay, A. A.; Purtov, K. V.; Petushkov, V. N.; Rodionova, N. S.; Yampolsky, I. V.

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20.


   
    Unusual shift in the visible absorption spectrum of an active ctenophore photoprotein elucidated by time-dependent density functional theory / F. N. Tomilin, A. V. Rogova, L. P. Burakova [et al.] // Photochem. Photobiol. Sci. - 2021. - Vol. 20, Is. 4. - P559-570, DOI 10.1007/s43630-021-00039-5. - Cited References:61. - The ab initio quantum chemical calculations were funded by RFBR and NSFC as the research project No. 19-54-53004 and RFBR research project No. 20-04-00085. The development of structural atomistic model of berovin without calcium ions generated by the I-TASSER server was funded by project 0721-2020-0033 of the Russian Ministry of Science and Education. . - ISSN 1474-905X. - ISSN 1474-9092
РУБ Biochemistry & Molecular Biology + Biophysics + Chemistry, Physical

Аннотация: Active hydromedusan and ctenophore Ca2+-regulated photoproteins form complexes consisting of apoprotein and strongly non-covalently bound 2-hydroperoxycoelenterazine (an oxygenated intermediate of coelenterazine). Whereas the absorption maximum of hydromedusan photoproteins is at 460-470 nm, ctenophore photoproteins absorb at 437 nm. Finding out a physical reason for this blue shift is the main objective of this work, and, to achieve it, the whole structure of the protein-substrate complex was optimized using a linear scaling quantum-mechanical method. Electronic excitations pertinent to the spectra of the 2-hydroperoxy adduct of coelenterazine were simulated with time-dependent density functional theory. The dihedral angle of 60 degrees of the 6-(p-hydroxy)-phenyl group relative to the imidazopyrazinone core of 2-hydroperoxycoelenterazine molecule was found to be the key factor determining the absorption of ctenophore photoproteins at 437 nm. The residues relevant to binding of the substrate and its adopting the particular rotation were also identified.

WOS
Держатели документа:
Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Kirensky Inst Phys SB RAS, Akademgorodok 50-38, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Svobodny 79 Pr, Krasnoyarsk 660041, Russia.
Natl Res Tomsk State Univ, Lenin Ave 36, Tomsk 634050, Russia.
Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Photobiol Lab, Inst Biophys SB RAS, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.
Kyungpook Natl Univ, 80 Daehakro, Daegu 41566, South Korea.
Natl Inst Adv Ind Sci & Technol, Res Ctr Computat Design Adv Funct Mat CD FMat, Cent 2,Umezono 1-1-1, Tsukuba, Ibaraki 3058568, Japan.

Доп.точки доступа:
Tomilin, Felix N.; Rogova, Anastasia V.; Burakova, Ludmila P.; Tchaikovskaya, Olga N.; Avramov, Pavel V.; Fedorov, Dmitri G.; Vysotski, Eugene S.; Burakova, Lyudmila; Vysotski, Eugene; Anastasia, Rogova; Tomilin, Felix; RFBRRussian Foundation for Basic Research (RFBR) [20-04-00085]; NSFCNational Natural Science Foundation of China (NSFC) [19-54-53004]; Russian Ministry of Science and EducationMinistry of Education and Science, Russian Federation [0721-2020-0033]

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