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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kovalev V. S., Grandl W., Manukovsky N. S., Tikhomirov A. A., Bock C.
Заглавие : Modeling a lunar base mushroom farm
Колич.характеристики :6 с
Место публикации : Life Sci. Space Res.: ELSEVIER, 2022. - Vol. 33. - С. 1-6. - ISSN 2214-5524, DOI 10.1016/j.lssr.2021.12.005. - ISSN 2214-5532(eISSN)
Примечания : Cited References:31
Предметные рубрики: SUPPORT
CULTIVATION
Аннотация: To calculate the equivalent system mass of mushrooms, a conceptual configuration of a mushroom farm as part of a bioregenerative life support system on an inhabited lunar base was designed. The mushroom farm consists of two connected modules. Each module is a double-shell rigid pipe-in-pipe aluminum structure. The first module is used to prepare and sterilize the substrate, while the mushrooms are sown and grown in the second module. Planned productivity of the mushroom farm is 28 kg of fresh mushrooms per one process cycle lasting 66 days for 14 consumers. Mushroom production can be increased using additional modules. The calculated equivalent system masses of the mushroom farm and the mushrooms produced therein is 88,432 kg and 31,550 kg per 1 kg of dry mushrooms in one process cycle, respectively. At that, the biggest contributor to the equivalent system mass of mushrooms is the total pressurized volume of the farm - 68%. The results obtained may be a prerequisite for performing trade-off studies between different configurations of mushroom farm and calculating a space diet using the equivalent system mass of mushrooms.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Golubev A. I., Nemtsev I. V., Lukyanenko A. V., Dudaev A. E., Shishatskaya E. I.
Заглавие : Laser processing of polymer films fabricated from phas differing in their monomer composition
Место публикации : Polym.: MDPI AG, 2021. - Vol. 13, Is. 10. - Ст.1553. - ISSN 20734360 (ISSN), DOI 10.3390/polym13101553
Аннотация: The study reports results of using a CO2-laser in continuous wave (3 W; 2 m/s) and quasi-pulsed (13.5 W; 1 m/s) modes to treat films prepared by solvent casting technique from four types of polyhydroxyalkanoates (PHAs), namely poly-3-hydroxybutyrate and three copolymers of 3-hydroxybutyrate: with 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate (each second monomer constituting about 30 mol.%). The PHAs differed in their thermal and molecular weight properties and degree of crystallinity. Pristine films differed in porosity, hydrophilicity, and roughness parameters. The two modes of laser treatment altered these parameters and biocompatibility in diverse ways. Films of P(3HB) had water contact angle and surface energy of 92? and 30.8 mN/m, respectively, and average roughness of 144 nm. The water contact angle of copolymer films decreased to 80–56? and surface energy and roughness increased to 41–57 mN/m and 172–290 nm, respectively. Treatment in either mode resulted in different modifications of the films, depending on their composition and irradiation mode. Laser-treated P(3HB) films exhibited a decrease in water contact angle, which was more considerable after the treatment in the quasi-pulsed mode. Roughness parameters were changed by the treatment in both modes. Continuous wave line-by-line irradiation caused formation of sintered grooves on the film surface, which exhibited some change in water contact angle (76–80? ) and reduced roughness parameters (to 40–45 mN/m) for most films. Treatment in the quasi-pulsed raster mode resulted in the formation of pits with no pronounced sintered regions on the film surface, a more considerably decreased water contact angle (to 67–76? ), and increased roughness of most specimens. Colorimetric assay for assessing cell metabolic activity (MTT) in NIH 3T3 mouse fibroblast culture showed that the number of fibroblasts on the films treated in the continuous wave mode was somewhat lower; treatment in quasi-pulsed radiation mode caused an increase in the number of viable cells by a factor of 1.26 to 1.76, depending on PHA composition. This is an important result, offering an opportunity of targeted surface modification of PHA products aimed at preventing or facilitating cell attachment. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana G., Golubev, Alexey, I, Nemtsev, Ivan, V, Lukyanenko, Anna, V, Dudaev, Alexey E., Shishatskaya, Ekaterina, I
Заглавие : Laser Processing of Polymer Films Fabricated from PHAs Differing in Their Monomer Composition
Колич.характеристики :24 с
Коллективы : Russian Foundation for Basic Research/Regional State Autonomous Institution "Krasnoyarsk Regional Fund for the Support of Scientific and ScientificTechnical Activities" foundations [19-43-240012]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Polymers: MDPI, 2021. - Vol. 13, Is. 10. - Ст.1553. - ISSN 2073-4360(eISSN), DOI 10.3390/polym13101553
Примечания : Cited References:87. - This work was supported by the Russian Foundation for Basic Research/Regional State Autonomous Institution "Krasnoyarsk Regional Fund for the Support of Scientific and ScientificTechnical Activities" foundations under Grant number 19-43-240012 (laser treatment and films properties) and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (polymer synthesis).
Предметные рубрики: CHEMOMECHANICAL PROPERTIES
PHYSICOCHEMICAL PROPERTIES
SURFACE
Аннотация: The study reports results of using a CO2-laser in continuous wave (3 W; 2 m/s) and quasi-pulsed (13.5 W; 1 m/s) modes to treat films prepared by solvent casting technique from four types of polyhydroxyalkanoates (PHAs), namely poly-3-hydroxybutyrate and three copolymers of 3-hydroxybutyrate: with 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate (each second monomer constituting about 30 mol.%). The PHAs differed in their thermal and molecular weight properties and degree of crystallinity. Pristine films differed in porosity, hydrophilicity, and roughness parameters. The two modes of laser treatment altered these parameters and biocompatibility in diverse ways. Films of P(3HB) had water contact angle and surface energy of 92 degrees and 30.8 mN/m, respectively, and average roughness of 144 nm. The water contact angle of copolymer films decreased to 80-56 degrees and surface energy and roughness increased to 41-57 mN/m and 172-290 nm, respectively. Treatment in either mode resulted in different modifications of the films, depending on their composition and irradiation mode. Laser-treated P(3HB) films exhibited a decrease in water contact angle, which was more considerable after the treatment in the quasi-pulsed mode. Roughness parameters were changed by the treatment in both modes. Continuous wave line-by-line irradiation caused formation of sintered grooves on the film surface, which exhibited some change in water contact angle (76-80 degrees) and reduced roughness parameters (to 40-45 mN/m) for most films. Treatment in the quasi-pulsed raster mode resulted in the formation of pits with no pronounced sintered regions on the film surface, a more considerably decreased water contact angle (to 67-76 degrees), and increased roughness of most specimens. Colorimetric assay for assessing cell metabolic activity (MTT) in NIH 3T3 mouse fibroblast culture showed that the number of fibroblasts on the films treated in the continuous wave mode was somewhat lower; treatment in quasi-pulsed radiation mode caused an increase in the number of viable cells by a factor of 1.26 to 1.76, depending on PHA composition. This is an important result, offering an opportunity of targeted surface modification of PHA products aimed at preventing or facilitating cell attachment.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tomilin, Felix N., Rogova, Anastasia V., Burakova, Ludmila P., Tchaikovskaya, Olga N., Avramov, Pavel V., Fedorov, Dmitri G., Vysotski, Eugene S.
Заглавие : Unusual shift in the visible absorption spectrum of an active ctenophore photoprotein elucidated by time-dependent density functional theory
Колич.характеристики :12 с
Коллективы : RFBRRussian Foundation for Basic Research (RFBR) [20-04-00085]; NSFCNational Natural Science Foundation of China (NSFC) [19-54-53004]; Russian Ministry of Science and EducationMinistry of Education and Science, Russian Federation [0721-2020-0033]
Место публикации : Photochem. Photobiol. Sci.: SPRINGERNATURE, 2021. - Vol. 20, Is. 4. - С. 559-570. - ISSN 1474-905X, DOI 10.1007/s43630-021-00039-5. - ISSN 1474-9092(eISSN)
Примечания : Cited References:61. - The ab initio quantum chemical calculations were funded by RFBR and NSFC as the research project No. 19-54-53004 and RFBR research project No. 20-04-00085. The development of structural atomistic model of berovin without calcium ions generated by the I-TASSER server was funded by project 0721-2020-0033 of the Russian Ministry of Science and Education.
Аннотация: Active hydromedusan and ctenophore Ca2+-regulated photoproteins form complexes consisting of apoprotein and strongly non-covalently bound 2-hydroperoxycoelenterazine (an oxygenated intermediate of coelenterazine). Whereas the absorption maximum of hydromedusan photoproteins is at 460-470 nm, ctenophore photoproteins absorb at 437 nm. Finding out a physical reason for this blue shift is the main objective of this work, and, to achieve it, the whole structure of the protein-substrate complex was optimized using a linear scaling quantum-mechanical method. Electronic excitations pertinent to the spectra of the 2-hydroperoxy adduct of coelenterazine were simulated with time-dependent density functional theory. The dihedral angle of 60 degrees of the 6-(p-hydroxy)-phenyl group relative to the imidazopyrazinone core of 2-hydroperoxycoelenterazine molecule was found to be the key factor determining the absorption of ctenophore photoproteins at 437 nm. The residues relevant to binding of the substrate and its adopting the particular rotation were also identified.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Beregovaya K. A., Myshkina N. M., Chepurnykh T. V., Kotlobay A. A., Purtov K. V., Petushkov V. N., Rodionova N. S., Yampolsky I. V.
Заглавие : Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi
Место публикации : Doklad. Biochem. Biophys.: Pleiades journals, 2021. - Vol. 496, Is. 1. - С. 14-17. - ISSN 16076729 (ISSN), DOI 10.1134/S1607672921010026
Аннотация: Abstract: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination. © 2021, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Beregovaya K. A., Myshkina N. M., Chepurnykh, T., V, Kotlobay A. A., Purtov, K., V, Petushkov V. N., Rodionova N. S., Yampolsky, I., V
Заглавие : Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi
Колич.характеристики :4 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-00052P]; President grant for leading scientific schoolsLeading Scientific Schools Program [NSh-2605.2020.4]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 496, Is. 1. - С. 14-17. - ISSN 1607-6729, DOI 10.1134/S1607672921010026. - ISSN 1608-3091(eISSN)
Примечания : Cited References:12. - This work was supported by the grant from the Russian Science Foundation no. 16-14-00052P, alanine screening was performed by the President grant for leading scientific schools NSh-2605.2020.4.
Аннотация: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pilla, Rachel M., Mette, Elizabeth M., Williamson, Craig E., Adamovich, Boris V., Adrian, Rita, Anneville, Orlane, Balseiro, Esteban, Ban, Syuhei, Chandra, Sudeep, Colom-Montero, William, Devlin, Shawn P., Dix, Margaret A., Dokulil, Martin T., Feldsine, Natalie A., Feuchtmayr, Heidrun, Fogarty, Natalie K., Gaiser, Evelyn E., Girdner, Scott F., Gonzalez, Maria J., Hambright, K. David, Hamilton, David P., Havens, Karl, Hessen, Dag O., Hetzenauer, Harald, Higgins, Scott N., Huttula, Timo H., Huuskonen, Hannu, Isles, Peter D. F., Joehnk, Klaus D., Keller, Wendel Bill, Klug, Jen, Knoll, Lesley B., Korhonen, Johanna, Korovchinsky, Nikolai M., Koster, Oliver, Kraemer, Benjamin M., Leavitt, Peter R., Leoni, Barbara, Lepori, Fabio, Lepskaya, Ekaterina V., Lottig, Noah R., Luger, Martin S., Maberly, Stephen C., MacIntyre, Sally, McBride, Chris, McIntyre, Peter, Melles, Stephanie J., Modenutti, Beatriz, Muller-Navarra L., Pacholski, Laura, Paterson, Andrew M., Pierson, Don C., Pislegina, Helen V., Plisnier, Pierre-Denis, Richardson, David C., Rimmer, Alon, Rogora, Michela, Rogozin, Denis Y., Rusak, James A., Rusanovskaya, Olga O., Sadro, Steve, Salmaso, Nico, Saros, Jasmine E., Sarvala, Jouko, Saulnier-Talbot, Emilie, Schindler, Daniel E., Shimaraeva, Svetlana V., Silow, Eugene A., Sitoki, Lewis M., Sommaruga, Ruben, Straile, Dietmar, Strock, Kristin E., Swain, Hilary, Tallant, Jason M., Thiery, Wim, Timofeyev, Maxim A., Tolomeev, Alexander P., Tominaga, Koji, Vanni, Michael J., Verburg, Piet, Vinebrooke, Rolf D., Wanzenbock, Josef, Weathers, Kathleen, Weyhenmeyer, Gesa A., Zadereev, Egor S., Zhukova, Tatyana V.
Заглавие : Global data set of long-term summertime vertical temperature profiles in 153 lakes
Колич.характеристики :12 с
Коллективы : Belarus Republican Foundation for Fundamental Research; AnaEE-France; SILA (Syndicat Mixte du Lac d'Annecy); Castle Lake Environmental Research and Education Program, University of Nevada at Reno; EC project "Response of European Freshwater Lakes [ENV4-CT97-0453]; EC-project "Climate Impacts on European Lakes" [EVK1-CT-2002-00121]; Austrian Climate and Energy Fund [K09ACK00046]; NSFNational Science Foundation (NSF) [DEB 1950170]; Crater Lake National Park Long-Term Limnological Monitoring Program; Ministry of Business, Innovation and Employment: Enhancing the Health and Resilience of New Zealand lakes [UOWX1503]; National Capital Authority; ACT, Australia [GB/NERC/BAS/AEDC/00063]; Belgian Science Policy OfficeBelgian Federal Science Policy Office; North Temperate Lakes LTER NTL-LTER [1440297]; NSERC CanadaNatural Sciences and Engineering Research Council of Canada (NSERC); Canada Research Chairs, Canada Foundation for InnovationCanada Foundation for InnovationCanada Research Chairs; University of Regina; Commissione Internazionale per la protezione delle acque italo-svizzere; Natural Environment Research CouncilUK Research & Innovation (UKRI)Natural Environment Research Council (NERC) [NE/R016429/1]; U.S. NSF Arctic LTER [DEB1637459, LTER_EU_IT_008]; Canton of Zurich [18-44-06201, 20-64-46003]; Russian Ministry of Higher Education and Research [FZZE-2020-0026, FZZE-2020-0023]; National Science Foundation Long Term Research in Environmental Biology program [DEB-1242626]; National Park Service (the Inventory and Monitoring Program); Acadia National Park monitoring program; Gordon and Betty Moore FoundationGordon and Betty Moore Foundation; Andrew Mellon Foundation; US National Science FoundationNational Science Foundation (NSF) [9318452, 9726877, 0235755, 0743192, 1255159]; Institut fur Seenforschung, Langenargen (Internationale Gewasserschutzkommission fur den Bodensee -IGKB); University of Michigan Biological StationUniversity of Michigan System; Belgian Science Policy Office (BELSPO)Belgian Federal Science Policy Office [CD/AR/02 A]; Waikato Regional Council; NIWA; Swedish Research CouncilSwedish Research CouncilEuropean Commission [2017-00635, NSF DEB 1754276]; Lacawac Sanctuary and Biological Field Station; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-04-00362 A, 19-05-00428]
Место публикации : Sci. Data: NATURE PORTFOLIO, 2021. - Vol. 8, Is. 1. - Ст.200. - ISSN 2052-4463(eISSN), DOI 10.1038/s41597-021-00983-y
Примечания : Cited References:45. - This work was conceived at the Global Lake Ecological Observatory Network (GLEON), and benefited from continued participation and travel support from GLEON. This manuscript is dedicated to the late Karl Havens and Alon Rimmer, who provided data for this manuscript. Funding and support for this work came from the following sources: the Belarus Republican Foundation for Fundamental Research; the IGB Long-term Ecological Research Programme; SOERE OLA, AnaEE-France, INRA Thonon les Bains, SILA (Syndicat Mixte du Lac d'Annecy), CISALB (Comite Intercommunautaire pour l'Assainissement du Lac du Bourget), and CIPEL (Commission Internationale pour la protection des eaux du Leman); Shiga Prefectural Fisheries Experiment Station (SPFES); Castle Lake Environmental Research and Education Program, University of Nevada at Reno and UC Davis; the Flathead Lake Monitoring program funded through a consortium of state and private funds, and thank the generous citizens of Flathead Lake for their continued support of lake monitoring; the Institute for water ecology, fish biology and lake research and the Institute for Limnology of the Austrian Academy of Sciences (until 2011), and acknowledge the sampling efforts by many individuals over the long period of investigation, especially H. Gassner, M. Luger, H. Ficker, and R. Kurmayer; the EC project "Response of European Freshwater Lakes to Environmental and Climatic Change" (REFLECT, ENV4-CT97-0453), the EC-project "Climate Impacts on European Lakes" (CLIME, EVK1-CT-2002-00121), the project "Risk Analysis of Direct and Indirect Climate effects on deep Austrian Lake Ecosystems" (RADICAL) funded by the Austrian Climate and Energy Fund (No. K09ACK00046) -Austrian Climate Research Programme (ACRP, http://www.klimafonds.gv.at); O. Garcia and E. Bocel for data analysis and management; D. Cabrera, M.W. Dix, G. Ochaeta, S. van Tuylen, M. Orozco, E. Symonds for sampling efforts; NSF grant No. 0947096 to E. Rejmankova, U.S. PeaceCorps and Ministerio de Ambiente y Recursos Naturales of Guatemala; H. Swain, L. Battoe, K. Main, N. Deyrup (Archbold Biological Station), the Florida Lakewatch program, E. Gaiser (Florida International University); the Crater Lake National Park Long-Term Limnological Monitoring Program; the City of Tulsa (R. West and A. Johnson), the Grand River Dam Authority (R. M. Zamor), W.M. Matthews and US ACE (T. Clyde), and the Oklahoma Water Resources Board; Bay of Plenty Regional Council; Ministry of Business, Innovation and Employment: Enhancing the Health and Resilience of New Zealand lakes (UOWX1503); the field and laboratory staff of the South Florida Water Management District for collecting and analyzing the samples; the Norwegian Water Resources and Energy Directorate (NVE), by courtesy of A. S. Kvambekk; the Lake Champlain Long-term Monitoring program (VT DEC and NY DEC); the National Capital Authority, ACT, Australia; Ontario Ministry of Environment, Conservation and Parks; FirstLight Power Resources and Friends of the Lake, especially G. Bollard and R. White; the Finnish Environment Institute SYKE database (Hertta) and S. Mitikka; N. Spinelli and the Lake Wallenpaupack Watershed Management District; Lakes Heywood, Moss, and Sombre: Long-Term Monitoring of Signy Lake Chemistry by BAS 1963-2004. Ref: GB/NERC/BAS/AEDC/00063, and dataset supplied by the Polar Data Centre under Open Government License (c) NERC-BAS, Lake Nkugute: Beadle (1966), CLANIMAE project funded by the Belgian Science Policy Office; Dr. L.; Garibaldi; NSF awards #1418698 and North Temperate Lakes LTER NTL-LTER #1440297; NSERC Canada, Canada Research Chairs, Canada Foundation for Innovation, Province of Saskatchewan, University of Regina, and Queen's University Belfast; Commissione Internazionale per la protezione delle acque italo-svizzere, Ufficio della protezione delle acque e dell'approvvigionamento idrico del Canton Ticino; KamchatNIRO scientists; Natural Environment Research Council award number NE/R016429/1 as part of the UK-SCaPE programme delivering National Capability; U.S. NSF Arctic LTER DEB1637459; Belgian Science Policy (Choltic, Climlake, Climfish); Ontario Ministry of Natural Resources' Harkness Laboratory of Fisheries Research, especially T. Middel; Max-Planck-Institute for Limnology Plon; staff at Erken Laboratory; Mohonk Preserve and D. Smiley; Lake Sunapee Protective Association; KLL database; International Commission for the Protection of Swiss-Italian Waters (CIPAIS) and the LTER (Long Term Ecological Research) Italian network, site "Southern Alpine lakes", LTER_EU_IT_008; staff and students at MECP's Dorset Environmental Science Centre; the LTER (Long-Term Ecological Research) Italian network, site "Southern Alpine lakes", IT08-005-A (http://www.lteritalia.it), with the support of the ARPA Veneto; Prof. L. Chapman, McGill University (Montreal, Quebec, Canada); Amt fur Abfall, Wasser, Energie und Luft (AWEL) of the Canton of Zurich; grants of RSCF project #18-44-06201 and #20-64-46003, of Russian Ministry of Higher Education and Research (projects. FZZE-2020-0026;. FZZE-2020-0023), and of Foundation for support of applied ecological studies "Lake Baikal" (https://baikalfoundation.ru/project/tochka-1/); National Science Foundation Long Term Research in Environmental Biology program (DEB-1242626); the National Park Service (the Inventory and Monitoring Program as well as the Air Resources Division) and Acadia National Park and the Acadia National Park monitoring program; Gordon and Betty Moore Foundation, the Andrew Mellon Foundation, the US National Science Foundation and the Bristol Bay salmon processors; J. Franzoi, G. Larsen, and S. Morales, and the LTSER platform Tyrolean Alps, which belongs to the national and international long-term ecological research network (LTER-Austria, LTER Europe and ILTER); Institut fur Seenforschung, Langenargen (Internationale Gewasserschutzkommission fur den Bodensee -IGKB); University of Michigan Biological Station (A. Schubel) and Cooperative Institute for Great Lakes Research (R. Miller); the Belgian Science Policy Office (BELSPO) is acknowledged for supporting research on Lake Kivu through the research project EAGLES (CD/AR/02 A); US National Science Foundation awards 9318452, 9726877, 0235755, 0743192 and 1255159; West Coast Regional Council, the Bay of Plenty Regional Council, and Waikato Regional Council, and NIWA; D. Schindler (funding and data access) and B. Parker (logistical support and data management); Swedish Infrastructure for Ecosystem Science (SITES) and the Swedish Research Council under the grant no 2017-00635; NSF DEB 1754276 and NSF DEB 1950170, the Ohio Eminent Scholar in Ecosystem Ecology fund, and Lacawac Sanctuary and Biological Field Station; Russian Foundation for Basic Research, grant. 19-04-00362 A and. 19-05-00428.
Предметные рубрики: CLIMATE-CHANGE
THERMAL STRATIFICATION
OXYGEN DEPLETION
FISH
Аннотация: Climate change and other anthropogenic stressors have led to long-term changes in the thermal structure, including surface temperatures, deepwater temperatures, and vertical thermal gradients, in many lakes around the world. Though many studies highlight warming of surface water temperatures in lakes worldwide, less is known about long-term trends in full vertical thermal structure and deepwater temperatures, which have been changing less consistently in both direction and magnitude. Here, we present a globally-expansive data set of summertime in-situ vertical temperature profiles from 153 lakes, with one time series beginning as early as 1894. We also compiled lake geographic, morphometric, and water quality variables that can influence vertical thermal structure through a variety of potential mechanisms in these lakes. These long-term time series of vertical temperature profiles and corresponding lake characteristics serve as valuable data to help understand changes and drivers of lake thermal structure in a time of rapid global and ecological change.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhou, Yali, Daver, Henrik, Trapkov, Boris, Wu, Lijie, Wu, Meng, Harpsoe, Kasper, Gentry, Patrick R., Liu, Kaiwen, Larionova, Marina, Liu, Junlin, Chen, N.a., Brauner-Osborne, Hans, Gloriam, David E., Hua, Tian, Liu, Zhi-Jie
Заглавие : Molecular insights into ligand recognition and G protein coupling of the neuromodulatory orphan receptor GPR139
Колич.характеристики :4 с
Коллективы : CAS Strategic Priority Research Program [XDB37030104]; National Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars [32022038]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930060, 31870744]; Shanghai Rising-Star Program [20QA1406500]; Lundbeck FoundationLundbeckfonden [R355-2020-949, R163-2013-16327]; Novo Nordisk FoundationNovo Nordisk FoundationNovocure Limited [NNF18OC0031226]; Independent Research Fund Denmark | Natural Sciences [8021-00173B]; Carlsberg FoundationCarlsberg Foundation [CF20-0248]
Место публикации : Cell Res.: SPRINGERNATURE, 2021. - Article in press. - ISSN 1001-0602, DOI 10.1038/s41422-021-00591-w. - ISSN 1748-7838(eISSN)
Примечания : Cited References:16. - This work was supported by the CAS Strategic Priority Research Program XDB37030104 (Z.-J.L.), the National Science Fund for Distinguished Young Scholars 32022038 (T.H.), the National Natural Science Foundation of China grants 31930060 (Z.-J.L.) and 31870744 (T.H.), and the Shanghai Rising-Star Program 20QA1406500 (T. H.), the Lundbeck Foundation R163-2013-16327 (D.E.G.), the Novo Nordisk Foundation NNF18OC0031226 (D.E.G.) and Independent Research Fund Denmark | Natural Sciences 8021-00173B (D.E.G.), the Lundbeck Foundation R355-2020-949 (B.T.) and the Carlsberg Foundation CF20-0248 (H.B.-O.). D.E.G. is a member of the Integrative Structural Biology at the University of Copenhagen (ISBUC). The cryo-EM data were collected at the Bio-Electron Microscopy Facility, ShanghaiTech University, with the assistance of Q.-Q. Sun, D.-D. Liu, Z.-H. Zhang and Y.-H. Liu. We thank the Assay Core, the assistance of F.-F. Zhou and Q.-W. Tan and the Cell Expression, Cloning and Purification Core Facilities of iHuman Institute for their support.
Предметные рубрики: DISCOVERY
PEPTIDES
ALPHA
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pilla R. M., Mette E. M., Williamson C. E., Adamovich B. V., Adrian R., Anneville O., Balseiro E., Ban S., Chandra S., Colom-Montero W., Devlin S. P., Dix M. A., Dokulil M. T., Feldsine N. A., Feuchtmayr H., Fogarty N. K., Gaiser E. E., Girdner S. F., Gonzalez M. J., Hambright K. D., Hamilton D. P., Havens K., Hessen D. O., Hetzenauer H., Higgins S. N., Huttula T. H., Huuskonen H., Isles P. D.F., Joehnk K. D., Keller W. B., Klug J., Knoll L. B., Korhonen J., Korovchinsky N. M., Koster O., Kraemer B. M., Leavitt P. R., Leoni B., Lepori F., Lepskaya E. V., Lottig N. R., Luger M. S., Maberly S. C., MacIntyre S., McBride C., McIntyre P., Melles S. J., Modenutti B., Muller-Navarra D. C., Pacholski L., Paterson A. M., Pierson D. C., Pislegina H. V., Plisnier P. -D., Richardson D. C., Rimmer A., Rogora M., Rogozin D. Y., Rusak J. A., Rusanovskaya O. O., Sadro S., Salmaso N., Saros J. E., Sarvala J., Saulnier-Talbot E., Schindler D. E., Shimaraeva S. V., Silow E. A., Sitoki L. M., Sommaruga R., Straile D., Strock K. E., Swain H., Tallant J. M., Thiery W., Timofeyev M. A., Tolomeev A. P., Tominaga K., Vanni M. J., Verburg P., Vinebrooke R. D., Wanzenbock J., Weathers K., Weyhenmeyer G. A., Zadereev E. S., Zhukova T. V.
Заглавие : Global data set of long-term summertime vertical temperature profiles in 153 lakes
Место публикации : Sci. Data: Nature Research, 2021. - Vol. 8, Is. 1. - Ст.200. - ISSN 20524463 (ISSN), DOI 10.1038/s41597-021-00983-y
Аннотация: Climate change and other anthropogenic stressors have led to long-term changes in the thermal structure, including surface temperatures, deepwater temperatures, and vertical thermal gradients, in many lakes around the world. Though many studies highlight warming of surface water temperatures in lakes worldwide, less is known about long-term trends in full vertical thermal structure and deepwater temperatures, which have been changing less consistently in both direction and magnitude. Here, we present a globally-expansive data set of summertime in-situ vertical temperature profiles from 153 lakes, with one time series beginning as early as 1894. We also compiled lake geographic, morphometric, and water quality variables that can influence vertical thermal structure through a variety of potential mechanisms in these lakes. These long-term time series of vertical temperature profiles and corresponding lake characteristics serve as valuable data to help understand changes and drivers of lake thermal structure in a time of rapid global and ecological change. © 2021, The Author(s).
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Esimbekova E. N., Kalyabina V. P., Kopylova K. V., Torgashina I. G., Kratasyuk V. A.
Заглавие : Design of bioluminescent biosensors for assessing contamination of complex matrices
Место публикации : Talanta: Elsevier B.V., 2021. - Vol. 233. - Ст.122509. - ISSN 00399140 (ISSN), DOI 10.1016/j.talanta.2021.122509
Примечания : Cited By :1
Аннотация: The presence of potentially toxic xenobiotics in complex matrices has become rather the rule than the exception. Therefore, there is a need for highly sensitive inexpensive techniques for analyzing environmental and food matrices for toxicants. Enzymes are selectively sensitive to various toxic compounds, and, thus, they can be used as the basis for detection of contaminants in complex matrices. There are, however, a number of difficulties associated with the analysis of complex matrices using enzyme assays, including the necessity to take into account properties and effects of the natural components of the test media for accurate interpretation of results. The present study describes the six-stage procedure for designing new enzyme sensors intended for assessing the quality of complex matrices. This procedure should be followed both to achieve the highest possible sensitivity of the biosensor to potentially toxic substances and to minimize the effect of the uncontaminated components of complex mixtures on the activity of the biosensor. The proposed strategy has been tested in designing a bioluminescent biosensor for integrated rapid assessment of the safety of fruits and vegetables. The biosensor is based on the coupled enzyme system NAD(P)H:FMN-oxidoreductase and luciferase as the biorecognition element. The study describes methods and techniques for attaining the desired result in each stage. The proposed six-stage procedure for designing bioluminescent enzyme biosensors can be used to design the enzymatic biosensors based on other enzymes. © 2021 Elsevier B.V.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Esimbekova, Elena N., Kalyabina, Valeriya P., Kopylova, Kseniya, V, Torgashina, Irina G., Kratasyuk, Valentina A.
Заглавие : Design of bioluminescent biosensors for assessing contamination of complex matrices
Колич.характеристики :9 с
Коллективы : Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR); Government of Krasnoyarsk Territory; Krasnoyarsk Regional Fund of Science [20-44-242001]; Ministry of Science and Higher Education of Russian Federation [FSRZ-2020-0006]
Место публикации : Talanta: ELSEVIER, 2021. - Vol. 233. - Ст.122509. - ISSN 0039-9140, DOI 10.1016/j.talanta.2021.122509. - ISSN 1873-3573(eISSN)
Примечания : Cited References:87. - The reported study was funded by Russian Foundation for Basic Research, Government of Krasnoyarsk Territory, Krasnoyarsk Regional Fund of Science, to the research project No. 20-44-242001 and Ministry of Science and Higher Education of Russian Federation No. FSRZ-2020-0006.
Предметные рубрики: SAMPLE PREPARATION
PESTICIDES
FOOD
BIOMOLECULES
SENSITIVITY
Аннотация: The presence of potentially toxic xenobiotics in complex matrices has become rather the rule than the exception. Therefore, there is a need for highly sensitive inexpensive techniques for analyzing environmental and food matrices for toxicants. Enzymes are selectively sensitive to various toxic compounds, and, thus, they can be used as the basis for detection of contaminants in complex matrices. There are, however, a number of difficulties associated with the analysis of complex matrices using enzyme assays, including the necessity to take into account properties and effects of the natural components of the test media for accurate interpretation of results. The present study describes the six-stage procedure for designing new enzyme sensors intended for assessing the quality of complex matrices. This procedure should be followed both to achieve the highest possible sensitivity of the biosensor to potentially toxic substances and to minimize the effect of the uncontaminated components of complex mixtures on the activity of the biosensor. The proposed strategy has been tested in designing a bioluminescent biosensor for integrated rapid assessment of the safety of fruits and vegetables. The biosensor is based on the coupled enzyme system NAD(P)H:FMN-oxidoreductase and luciferase as the biorecognition element. The study describes methods and techniques for attaining the desired result in each stage. The proposed six-stage procedure for designing bioluminescent enzyme biosensors can be used to design the enzymatic biosensors based on other enzymes.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Davydova, Anna, Krasitskaya, Vasilisa, Vorobjev, Pavel, Timoshenko, Valentina, Tupikin, Alexey, Kabilov, Marsel, Frank, Ludmila, Venyaminova, Alya, Vorobyeva, Mariya
Заглавие : Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays
Колич.характеристики :7 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-10296]; Russian State funded budget projects [AAAA-A17-117020210021-7, AAAA-A19-119031890015-0]
Место публикации : RSC Adv.: ROYAL SOC CHEMISTRY, 2020. - Vol. 10, Is. 54. - С. 32393-32399. - ISSN 2046-2069(eISSN), DOI 10.1039/d0ra05117a
Примечания : Cited References:33. - The work was supported by the Russian Science Foundation (grant #16-14-10296), Russian State funded budget projects #AAAA-A17-117020210021-7 to ICBFM SB RAS and #AAAA-A19-119031890015-0 to IBP SB RAS.
Предметные рубрики: DNA APTAMER
RNA APTAMER
OBELIN
PURIFICATION
EXPRESSION
SEQUENCES
Аннотация: We report a novel bioluminescent aptasensor, which consists of 2 '-F-RNA aptamer modules joined into a bi-specific aptamer construct. One aptamer module binds the analyte, then after structural rearrangement the second module recruits non-covalently Ca2+-dependent photoprotein obelin from the solution, thus providing a bioluminescent signal. This concept allows using free protein as a reporter, which brings such advantages as no need for aptamer-protein conjugation, a possibility of thermal re-folding of aptamer component with no harm to a protein, and simpler detection protocol. We developed the new 2 '-F-RNA aptamer for obelin, and proposed the strategy for engineering structure-switching bi-modular aptamer constructs which bind the analyte and the obelin in a sequential manner. With the use of hemoglobin as a model analyte, we showed the feasibility of utilizing the aptasensor in a fast and straightforward bioluminescent microplate assay. With a proper design of a secondary structure, this strategy of aptasensor engineering might be further extended to bi-specific aptamer-based bioluminescent sensors for other analytes of interest.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya, Vasilisa V., Goncharova, Natalia S., Biriukov, Vladislav V., Bashmakova, Eugenia E., Kabilov, Marsel R., Baykov, Ivan K., Sokolov, Aleksey E., Frank, Ludmila A.
Заглавие : The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation
Колич.характеристики :6 с
Коллективы : Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [18-38-00531]
Место публикации : Photochem. Photobiol.: WILEY, 2020. - Article in press. - ISSN 0031-8655, DOI 10.1111/php.13274. - ISSN 1751-1097(eISSN)
Примечания : Cited References:25. - This work has been supported by the Russian Foundation for Basic Research (RFBR) under the grant no 18-38-00531.
Предметные рубрики: CARDIAC TROPONIN-I
BIOLUMINESCENCE
IMMUNOASSAY
APTASENSOR
DIAGNOSIS
Аннотация: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Efremov A. A., Zykova I. D., Korosteleva N. S.
Заглавие : Antimicrobial and antiradical activity of individual fractions of essential oil from seeds of heracleum dissectum ledeb. Of Siberian Region
Место публикации : Khimiya Rastitel'nogo Syr'ya: Altai State University, 2020. - Is. 2. - С. 79-85. - ISSN 10295151 (ISSN), DOI 10.14258/JCPRM.2020027029
Аннотация: By the method of exhaustive hydroponically obtained essential oil from beans of Heracleum dissectum Ledeb., growing in the Krasnoyarsk region. Separate fractions of oil were obtained: the first after 45 minutes from the beginning of distillation, the second – after 2 hours, the third-after 5 hours, the fourth fraction was collected after the end of hydro-distillation. The component composition of both whole essential oil and its separate fractions was studied. The main components are octyl acetate (60.0%), octyl-2-methylpropanoate (10.2%), n-hexyl-2-methylbutanoate (9.0%). The main amount of octyl acetate (64.7%) is concentrated in the first fraction of the oil. The antimicrobial activity of various fractions of essential oil of borscht dissected against strains of opportunistic microorganisms: Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus 209p, MRSA, Proteus vulgaris. It was found that, depending on the duration of isolation, the antimicrobial activity of essential oil fractions in relation to Staphylococcus aureus 209p, MRSA and Pseudomonas aeruginosa decreases, and in relation to Escherichia coli, Klebsiella pneumoniae and Proteus vulgaris increases. The most pronounced inhibitory effect of the third and fourth fractions of essential oil against Klebsiella pneumonia. The antiradical activity of all studied samples of borscht essential oil dissected in reaction with stable free 2,2-diphenyl-1-picrylhydrazyl radical was established. The first fraction showed minimal antiradical activity (15.1%), the fourth – maximum (49.2%). © 2020 Altai State University. All rights reserved.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pilla, Rachel M., Williamson, Craig E., Adamovich, Boris V., Adrian, Rita, Anneville, Orlane, Chandra, Sudeep, Colom-Montero, William, Devlin, Shawn P., Dix, Margaret A., Dokulil, Martin T., Gaiser, Evelyn E., Girdner, Scott F., Hambright, K. David, Hamilton, David P., Havens, Karl, Hessen, Dag O., Higgins, Scott N., Huttula, Timo H., Huuskonen, Hannu, Isles, Peter D. F., Joehnk, Klaus D., Jones, Ian D., Keller, Wendel Bill, Knoll, Lesley B., Korhonen, Johanna, Kraemer, Benjamin M., Leavitt, Peter R., Lepori, Fabio, Luger, Martin S., Maberly, Stephen C., Melack, John M., Melles, Stephanie J., Muller-Navarra D. C., Pierson, Don C., Pislegina, Helen V., Plisnier, Pierre-Denis, Richardson, David C., Rimmer, Alon, Rogora, Michela, Rusak, James A., Sadro, Steven, Salmaso, Nico, Saros, Jasmine E., Saulnier-Talbot, Emilie, Schindler, Daniel E., Schmid, Martin, Shimaraeva, Svetlana V., Silow, Eugene A., Sitoki, Lewis M., Sommaruga, Ruben, Straile, Dietmar, Strock, Kristin E., Thiery, Wim, Timofeyev, Maxim A., Verburg, Piet, Vinebrooke, Rolf D., Weyhenmeyer, Gesa A., Zadereev, Egor
Заглавие : Deeper waters are changing less consistently than surface waters in a global analysis of 102 lakes
Колич.характеристики :15 с
Коллективы : Belarus Republican Foundation for Fundamental Research; IGB Long-Term Research; European CommissionEuropean CommissionEuropean Commission Joint Research Centre; DFGGerman Research Foundation (DFG) [AD 91/22-1]; OLA-IS; AnaEE-France; INRAE of Thonon-les-Bains; CIPEL; SILA; CISALB; Universidad del Valle de Guatemala; Archbold Biological Station; Oklahoma Department of Wildlife Conservation; Oklahoma Water Resources Board; Grand River Dam Authority; US Army Corps of EngineersUnited States Department of Defense; City of Tulsa; Ministry of Business, Innovation, and EmploymentNew Zealand Ministry of Business, Innovation and Employment (MBIE) [UOW X1503]; Natural Environment Research Council of the UKNERC Natural Environment Research Council; IGB's International Postdoctoral Fellowship; NSERCNatural Sciences and Engineering Research Council of Canada; Canada Foundation for InnovationCanada Foundation for InnovationCGIAR; Canada Research ChairsCanada Research ChairsCGIAR; Province of Saskatchewan; University of Regina; Queen's University Belfast; Natural Environment Research CouncilNERC Natural Environment Research Council; US-NSFNational Science Foundation (NSF); California Air Resources Board; NASANational Aeronautics & Space Administration (NASA); US National Park Service; Ministry of Higher Education and ResearchMinistry of Higher Education & Scientific Research (MHESR) [FZZE-2020-0026, FZZE-2020-0023]; RSCFRussian Science Foundation (RSF) [20-64-46003]; US National Science Foundation Long Term Research in Environmental Biology program [DEB-1242626]; Environmental Agency of Verona; US National Science FoundationNational Science Foundation (NSF); Gordon and Betty Moore FoundationGordon and Betty Moore Foundation; Mellon Foundation; University of WashingtonUniversity of Washington; KMFRI; LVEMP; University of Innsbruck; OeAD; IFSInternational Foundation for Science; LVFO-EU; Waikato Regional Council; Bay of Plenty Regional Council; Swedish Environmental Protection Agency; Swedish Infrastructure for Ecosystem Sciences; US National Science FoundationNational Science Foundation (NSF) [DEB-1754276, DEB-1950170]
Место публикации : Sci Rep: NATURE RESEARCH, 2020. - Vol. 10, Is. 1. - Ст.20514. - ISSN 2045-2322, DOI 10.1038/s41598-020-76873-x
Примечания : Cited References:87. - This work was conceived at the Global Lake Ecological Observatory Network (GLEON), and benefited from continued participation and travel support from GLEON. This manuscript is dedicated to the late Alon Rimmer and Karl Havens, who provided data and contributed to earlier versions of this manuscript. Funding in support of this work came from the following sources: Belarus Republican Foundation for Fundamental Research; IGB Long-Term Research; the European Commission within the MANTEL project; the DFG within the LimnoScenES project (AD 91/22-1); OLA-IS, AnaEE-France, INRAE of Thonon-les-Bains, CIPEL, SILA, CISALB; Universidad del Valle de Guatemala; Archbold Biological Station; the Oklahoma Department of Wildlife Conservation, the Oklahoma Water Resources Board, the Grand River Dam Authority, the US Army Corps of Engineers, and the City of Tulsa; the Ministry of Business, Innovation, and Employment (UOW X1503); the Natural Environment Research Council of the UK; the IGB's International Postdoctoral Fellowship; NSERC, Canada Foundation for Innovation, Canada Research Chairs, Province of Saskatchewan; University of Regina; Queen's University Belfast; Natural Environment Research Council; US-NSF, California Air Resources Board, NASA, and US National Park Service; the Ministry of Higher Education and Research (projects No FZZE-2020-0026; No FZZE-2020-0023) and RSCF 20-64-46003; US National Science Foundation Long Term Research in Environmental Biology program (DEB-1242626); the Environmental Agency of Verona; US National Science Foundation, the Gordon and Betty Moore Foundation, the Mellon Foundation, and the University of Washington; KMFRI, LVEMP, University of Innsbruck, OeAD, IFS, and LVFO-EU; Waikato Regional Council and Bay of Plenty Regional Council; Swedish Environmental Protection Agency and the Swedish Infrastructure for Ecosystem Sciences; US National Science Foundation grants DEB-1754276 and DEB-1950170. We thank J. Klug, P. McIntyre, H. Swain, K. Tominaga, A. Voutilainen, and L. Winslow for their feedback on early drafts that substantially improved this manuscript. Additional detailed acknowledgements can be found in the Supplementary Information online.
Предметные рубрики: DISSOLVED ORGANIC-CARBON
LONG-TERM CHANGES
CLIMATE-CHANGE
OXYGEN
Аннотация: Globally, lake surface water temperatures have warmed rapidly relative to air temperatures, but changes in deepwater temperatures and vertical thermal structure are still largely unknown. We have compiled the most comprehensive data set to date of long-term (1970-2009) summertime vertical temperature profiles in lakes across the world to examine trends and drivers of whole-lake vertical thermal structure. We found significant increases in surface water temperatures across lakes at an average rate of+0.37 degrees C decade(-1), comparable to changes reported previously for other lakes, and similarly consistent trends of increasing water column stability (+0.08 kg m(-3) decade(-1)). In contrast, however, deepwater temperature trends showed little change on average (+0.06 degrees C decade(-1)), but had high variability across lakes, with trends in individual lakes ranging from -0.68 degrees C decade(-1) to+0.65 degrees C decade(-1). The variability in deepwater temperature trends was not explained by trends in either surface water temperatures or thermal stability within lakes, and only 8.4% was explained by lake thermal region or local lake characteristics in a random forest analysis. These findings suggest that external drivers beyond our tested lake characteristics are important in explaining long-term trends in thermal structure, such as local to regional climate patterns or additional external anthropogenic influences.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pilla R. M., Williamson C. E., Adamovich B. V., Adrian R., Anneville O., Chandra S., Colom-Montero W., Devlin S. P., Dix M. A., Dokulil M. T., Gaiser E. E., Girdner S. F., Hambright K. D., Hamilton D. P., Havens K., Hessen D. O., Higgins S. N., Huttula T. H., Huuskonen H., Isles P. D.F., Joehnk K. D., Jones I. D., Keller W. B., Knoll L. B., Korhonen J., Kraemer B. M., Leavitt P. R., Lepori F., Luger M. S., Maberly S. C., Melack J. M., Melles S. J., Muller-Navarra D. C., Pierson D. C., Pislegina H. V., Plisnier P. -D., Richardson D. C., Rimmer A., Rogora M., Rusak J. A., Sadro S., Salmaso N., Saros J. E., Saulnier-Talbot E., Schindler D. E., Schmid M., Shimaraeva S. V., Silow E. A., Sitoki L. M., Sommaruga R., Straile D., Strock K. E., Thiery W., Timofeyev M. A., Verburg P., Vinebrooke R. D., Weyhenmeyer G. A., Zadereev E.
Заглавие : Deeper waters are changing less consistently than surface waters in a global analysis of 102 lakes
Место публикации : Sci. Rep.: Nature Research, 2020. - Vol. 10, Is. 1. - Ст.20514. - ISSN 20452322 (ISSN), DOI 10.1038/s41598-020-76873-x
Аннотация: Globally, lake surface water temperatures have warmed rapidly relative to air temperatures, but changes in deepwater temperatures and vertical thermal structure are still largely unknown. We have compiled the most comprehensive data set to date of long-term (1970–2009) summertime vertical temperature profiles in lakes across the world to examine trends and drivers of whole-lake vertical thermal structure. We found significant increases in surface water temperatures across lakes at an average rate of + 0.37 °C decade?1, comparable to changes reported previously for other lakes, and similarly consistent trends of increasing water column stability (+ 0.08 kg m?3 decade?1). In contrast, however, deepwater temperature trends showed little change on average (+ 0.06 °C decade?1), but had high variability across lakes, with trends in individual lakes ranging from ? 0.68 °C decade?1 to + 0.65 °C decade?1. The variability in deepwater temperature trends was not explained by trends in either surface water temperatures or thermal stability within lakes, and only 8.4% was explained by lake thermal region or local lake characteristics in a random forest analysis. These findings suggest that external drivers beyond our tested lake characteristics are important in explaining long-term trends in thermal structure, such as local to regional climate patterns or additional external anthropogenic influences. © 2020, The Author(s).
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Eremeeva, Elena V., Vysotski, Eugene S.
Заглавие : Exploring Bioluminescence Function of the Ca2+-regulated Photoproteins with Site-directed Mutagenesis
Колич.характеристики :16 с
Коллективы : Russian Foundation for Basic Research [17-04-00764]; Russian Academy of Sciences [0356-2017-0017]
Место публикации : Photochem. Photobiol.: WILEY, 2019. - Vol. 95, Is. 1. - С. 8-23. - ISSN 0031-8655, DOI 10.1111/php.12945. - ISSN 1751-1097(eISSN)
Примечания : Cited References:88. - This work was supported by grant 17-04-00764 of Russian Foundation for Basic Research and the state budgetallocated to the fundamental research at the Russian Academy of Sciences (project 0356-2017-0017).
Предметные рубрики: CALCIUM-BINDING PHOTOPROTEIN
GREEN-FLUORESCENT PROTEIN
JELLYFISH
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--aequorin--obelin--clytin--mitrocomin--ef-hand protein
Аннотация: Site-directed mutagenesis is a powerful tool to investigate the structure-function relationship of proteins and a function of certain amino acid residues in catalytic conversion of substrates during enzymatic reactions. Hence, it is not surprising that this approach was repeatedly applied to elucidate the role of certain amino acid residues in various aspects of photoprotein bioluminescence, mostly for aequorin and obelin, and to design mutant photoproteins with altered properties (modified calcium affinity, faster or slower bioluminescence kinetics, different emission color) which would either allow the development of novel bioluminescent assays or improvement of characteristics of the already existing ones. This information, however, is scattered over different articles. In this review, we systematize the findings that were made using site-directed mutagenesis studies regarding the impact of various amino acid residues on bioluminescence of hydromedusan Ca2+-regulated photoproteins. All key residues that have been identified are pinpointed, and their influence on different aspects of photoprotein functioning such as active photoprotein complex formation, bioluminescence reaction, calcium response and light emitter formation is discussed.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vyshedko A. M., Stepanova L. V., Kolenchukova O. A., Kratasyuk V. A.
Заглавие : Benefits of biophysical tests of saliva for athletic functionality rating tests
Место публикации : Teor. Prakt. Fiz. Kult.: Teoriya i praktika fizicheskoy kul'tury i sporta, 2019. - Vol. 2019, Is. 7. - С. 65-67. - ISSN 00403601 (ISSN)
Аннотация: Modern physical training systems give a high priority to the training process individualizing elements that may be efficient enough only when supported by objective tests to rate the bodily responses to physical workloads. Moreover, success in modern professional sports is impossible unless supported by the newest medical/ biological findings and technologies in the health rating and forecast domains, with a special role played by the modern biophysical research of the cellular- and molecular-level biological processes. The study was designed to assess benefits of biophysical analysis of saliva for the athletic functionality rating tests. Sampled for the purposes of the study were athletes of different skill levels (from Class I to Honorary Masters of Sport) versus the untrained students, with the saliva sampled prior to and after physical trainings by bioluminescence and chemoluminescence test methods. The test data were processed by Statistica 10 (made by StatSoft Inc., the US) toolkit to produce median values (Me) and inter-quartile distribution (C25-C75 percentile) rates. The Mann-Whitney nonparametric U-test of the null hypothesis was used to rate differences of the independent variables, with the statistical difference rated meaningful at p0.05. The bioluminescence tests found the higher ferment activity of saliva in the athletes’ versus untrained students, with the ferment activity in the bioluminescence tests also tested to grow after physical workloads in the highly-skilled athletes and fall in the untrained students. The study found the saliva antioxidant system functional intensity being athletic skills dependent. Therefore, the study has demonstrated benefits of the biophysical (bioluminescence and chemoluminescence) tests of saliva for the athletic functionality rating tests in the physical workload design and management initiatives. © 2019, Teoriya i praktika fizicheskoy kul'tury i sporta. All rights reserved.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Yu M., Benjamin M. M., Srinivasan S., Morin E. E., Shishatskaya E. I., Schwendeman S. P., Schwendeman A.
Заглавие : Battle of GLP-1 delivery technologies
Место публикации : Adv. Drug Deliv. Rev.: Elsevier B.V., 2018. - ISSN 0169409X (ISSN) , DOI 10.1016/j.addr.2018.07.009
Ключевые слова (''Своб.индексиров.''): albumin fusion--exenatide--fatty acid conjugate--fc fusion--glp-1 receptor agonist--half-life--peptide delivery--pharmacokinetics
Аннотация: Glucagon-like peptide-1 receptor agonists (GLP-1 RAs) belong to an important therapeutic class for treatment of type 2 diabetes. Six GLP-1 RAs, each utilizing a unique drug delivery strategy, are now approved by the Food and Drug Administration (FDA) and additional, novel GLP-1 RAs are still under development, making for a crowded marketplace and fierce competition among the manufacturers of these products. As rapid elimination is a major challenge for clinical application of GLP-1 RAs, various half-life extension strategies have been successfully employed including sequential modification, attachment of fatty-acid to peptide, fusion with human serum albumin, fusion with the fragment crystallizable (Fc) region of a monoclonal antibody, sustained drug delivery systems, and PEGylation. In this review, we discuss the scientific rationale of the various half-life extension strategies used for GLP-1 RA development. By analyzing and comparing different approved GLP-1 RAs and those in development, we focus on assessing how half-life extending strategies impact the pharmacokinetics, pharmacodynamics, safety, patient usability and ultimately, the commercial success of GLP-1 RA products. We also anticipate future GLP-1 RA development trends. Since similar drug delivery strategies are also applied for developing other therapeutic peptides, we expect this case study of GLP-1 RAs will provide generalizable concepts for the rational design of therapeutic peptides products with extended duration of action. © 2018 Elsevier B.V.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bashmakova E. E., Krasitskaya V. V., Bondar A. A., Eremina E. N., Slepov E. V., Zukov R. A., Frank L. A.
Заглавие : Bioluminescent SNP genotyping technique: Development and application for detection of melanocortin 1 receptor gene polymorphisms
Место публикации : Talanta: Elsevier B.V., 2018. - Vol. 189. - С. 111-115. - ISSN 00399140 (ISSN) , DOI 10.1016/j.talanta.2018.06.057
Ключевые слова (''Своб.индексиров.''): ca2+-regulated photoprotein obelin--genotyping--melanocortin 1 receptor gene--single nucleotide polymorphisms (snp)--bioluminescence--clinical research--curricula--diagnosis--genes--oncology--biomedical research--clinical characteristics--development and applications--genotyping--healthy individuals--photoproteins--receptor genes--single-nucleotide polymorphisms--dermatology
Аннотация: SNP genotyping based on the reaction of specific primer extension with the following bioluminescent detection of its products was shown to be potentially applicable for biomedical exploration. The paper describes its elaboration and first application in extensive biomedical research concerning MC1R gene variants’ frequency and associations with clinical characteristics in melanoma patients of Eastern Siberia (Krasnoyarsk region, Russia). Polymorphisms rs 1805007 (R151C), rs 1805008 (R160W), and rs 1805009 (D294H) were detected in 174 DNA samples from patients with histologically proved diagnosis of cutaneous melanoma and in 200 samples from healthy individuals. All the results on bioluminescent SNP genotyping were confirmed by Sanger sequencing. Some features characteristic of the population were found, i.e. melanoma is mostly associated with R160W or R151C while variant D294H is extremely rare; simultaneous carriage of any two investigated variants is also strongly associated with melanoma; R151C is associated with ulceration and consequently the disease course is more aggressive, etc. The design of the technique allows fast evaluation of any known diagnostically important SNP frequencies and associations across population. © 2018 Elsevier B.V.
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