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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tomilin, Felix N., Rogova, Anastasia V., Burakova, Ludmila P., Tchaikovskaya, Olga N., Avramov, Pavel V., Fedorov, Dmitri G., Vysotski, Eugene S.
Заглавие : Unusual shift in the visible absorption spectrum of an active ctenophore photoprotein elucidated by time-dependent density functional theory
Колич.характеристики :12 с
Коллективы : RFBRRussian Foundation for Basic Research (RFBR) [20-04-00085]; NSFCNational Natural Science Foundation of China (NSFC) [19-54-53004]; Russian Ministry of Science and EducationMinistry of Education and Science, Russian Federation [0721-2020-0033]
Место публикации : Photochem. Photobiol. Sci.: SPRINGERNATURE, 2021. - Vol. 20, Is. 4. - С. 559-570. - ISSN 1474-905X, DOI 10.1007/s43630-021-00039-5. - ISSN 1474-9092(eISSN)
Примечания : Cited References:61. - The ab initio quantum chemical calculations were funded by RFBR and NSFC as the research project No. 19-54-53004 and RFBR research project No. 20-04-00085. The development of structural atomistic model of berovin without calcium ions generated by the I-TASSER server was funded by project 0721-2020-0033 of the Russian Ministry of Science and Education.
Аннотация: Active hydromedusan and ctenophore Ca2+-regulated photoproteins form complexes consisting of apoprotein and strongly non-covalently bound 2-hydroperoxycoelenterazine (an oxygenated intermediate of coelenterazine). Whereas the absorption maximum of hydromedusan photoproteins is at 460-470 nm, ctenophore photoproteins absorb at 437 nm. Finding out a physical reason for this blue shift is the main objective of this work, and, to achieve it, the whole structure of the protein-substrate complex was optimized using a linear scaling quantum-mechanical method. Electronic excitations pertinent to the spectra of the 2-hydroperoxy adduct of coelenterazine were simulated with time-dependent density functional theory. The dihedral angle of 60 degrees of the 6-(p-hydroxy)-phenyl group relative to the imidazopyrazinone core of 2-hydroperoxycoelenterazine molecule was found to be the key factor determining the absorption of ctenophore photoproteins at 437 nm. The residues relevant to binding of the substrate and its adopting the particular rotation were also identified.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinova O.N., Kalacheva G.S., Grodnitskaya I.D.
Заглавие : The prospects of the use of resorbable polyesters for designing safe pesticides
Место публикации : Doklady Biological Sciences. - 2008. - Vol. 419, Is. 1. - С. 100-103. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496608020099
Ключевые слова (''Своб.индексиров.''): pesticide--polyester--article--chemistry--drug design--metabolism--microbiology--drug design--pesticides--polyesters--soil microbiology
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzir’ A. P., Minakov A. V., Burov A. E., Zharkov S. M., Maksimov N. G., Pryazhnikov M. I.
Заглавие : The effect of silver ions electrolytically introduced into colloidal nanodiamond solution on its viscosity and thermal conductivity
Место публикации : Colloid J.: Maik Nauka Publishing / Springer SBM, 2017. - Vol. 79, Is. 2. - С. 258-263. - ISSN 1061933X (ISSN) , DOI 10.1134/S1061933X17020119
Ключевые слова (''Своб.индексиров.''): dispersions--ions--metal ions--nanodiamonds--nanoparticles--silver--viscosity--a-stable--detonation nanodiamond--diamond nano-particles--effect of silvers--silver concentration--silver ions--thermal conductivity
Аннотация: Experimental data have been presented on the influence of silver on the viscosity and thermal conductivity of a dispersion of diamond nanoparticles. A stable dispersion (5 wt %) of detonation nanodiamond particles has been used in the experiments. Silver ions have been introduced electrolytically into the dispersion of diamond nanoparticles. Silver concentration was not higher than 0.05 wt %. It has been shown that the introduction of silver ions significantly affects the thermal conductivity and viscosity of the dispersion. © 2017, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vinnik I. S., Markelova N. M., Solov'Eva N. S., Shishatskaia E. I., Kuznetsov M. N., Zuev A. P.
Заглавие : The current dressings for wound care in the treatment of purulent wounds
Место публикации : Nov. Khir. - 2015. - Vol. 23, Is. 5. - С. 552-558. - ISSN 19937512 (ISSN) , DOI 10.18484/23050047.2015.5.552
Ключевые слова (''Своб.индексиров.''): biocompatible materials--healing and regeneration--purulent wound--treatment of wounds--wound covering--wound dressing--wound process
Аннотация: Currently, the patients with purulent wounds account for about 40% of the patients of surgical profilt. Since the treatment of septic wounds under the bandage remains the main method, as it is the most convenient and economically beneficial, but not effective enough, the development of new advanced coatings possessing simultaneously by multiple properties, is considered to be relevant. In the process of wound healing a physician faces with many problems including the issue of the selection option of a wound dressing, as they differ significantly in their design and properties. A disadvantage of many wound dressings for wound care is the adherence to wound. This results in the regenerating tissue injury and the dressings become sore. Many of the positive properties of dressings reduced as a result of damage caused by daily dressings. In recent years the biocompatible materials for injuries and wounds contribute to more effective healing and regeneration. In this regard, the paper presents the main types of current wound dressings used in the treatment of purulent wounds, their properties, indications and methods of applicatio.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya, Vasilisa V., Goncharova, Natalia S., Biriukov, Vladislav V., Bashmakova, Eugenia E., Kabilov, Marsel R., Baykov, Ivan K., Sokolov, Aleksey E., Frank, Ludmila A.
Заглавие : The Ca2+-Regulated Photoprotein Obelin as a Tool for SELEX Monitoring and DNA Aptamer Affinity Evaluation
Колич.характеристики :6 с
Коллективы : Russian Foundation for Basic Research (RFBR)Russian Foundation for Basic Research (RFBR) [18-38-00531]
Место публикации : Photochem. Photobiol.: WILEY, 2020. - Article in press. - ISSN 0031-8655, DOI 10.1111/php.13274. - ISSN 1751-1097(eISSN)
Примечания : Cited References:25. - This work has been supported by the Russian Foundation for Basic Research (RFBR) under the grant no 18-38-00531.
Предметные рубрики: CARDIAC TROPONIN-I
BIOLUMINESCENCE
IMMUNOASSAY
APTASENSOR
DIAGNOSIS
Аннотация: Bioluminescent solid-phase analysis was proposed to monitor the selection process and to determine binding characteristics of the aptamer-target complexes during design and development of the specific aptamers. The assay involves Ca2+-regulated photoprotein obelin as a simple, sensitive and fast reporter. Applicability and the prospects of the approach were exemplified by identification of DNA aptamers to cardiac troponin I, a highly specific early biomarker for acute myocardial infarction. Two structurally different aptamers specific to various epitopes of troponin I were obtained and then tested in a model bioluminescent assay.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Bartsev S.I., Mezhevikin V.V., Okhonin V.A.
Заглавие : Systematic approach to life support system analyses and integration
Колич.характеристики :10 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P1823-1832. - ISBN 0273-1177, DOI 10.1016/S0273-1177(03)00081-4
Примечания : Cited References: 25
Предметные рубрики: RELIABILITY
SPACE
Аннотация: This paper is devoted to the consideration of possible viewpoint on CELSS development and design. If the aim to create practically applicable CELSS is accepted then the task to optimize the process of CELSS research and development in terms of minimum cost, hours, maximum applicability, scientific contribution, etc. becomes actual. Requirements of applicability and scientific significance are synergetic since understanding of general properties of CELSS gives an ability to create CELSS for different applications. To accomplish the task three main groups of parameters have to be optimized: i) configuration and operating parameters of developing CELSS itself, ii) organizational management of research and development of CELSS; iii) features of an area where CELSS is planned to be used (space missions, terrestrial applications, or biosphere investigation) and where requirements to CELSS characteristic come from. Given paper is a brief review presented some attempts to arrange mentioned above into some set of formalized and interacting criteria, and some progression of research stages derived from these criteria. (C) 2003 Published by Elsevier Science Ltd on behalf of COSPAR.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Davydova, Anna, Krasitskaya, Vasilisa, Vorobjev, Pavel, Timoshenko, Valentina, Tupikin, Alexey, Kabilov, Marsel, Frank, Ludmila, Venyaminova, Alya, Vorobyeva, Mariya
Заглавие : Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays
Колич.характеристики :7 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-10296]; Russian State funded budget projects [AAAA-A17-117020210021-7, AAAA-A19-119031890015-0]
Место публикации : RSC Adv.: ROYAL SOC CHEMISTRY, 2020. - Vol. 10, Is. 54. - С. 32393-32399. - ISSN 2046-2069(eISSN), DOI 10.1039/d0ra05117a
Примечания : Cited References:33. - The work was supported by the Russian Science Foundation (grant #16-14-10296), Russian State funded budget projects #AAAA-A17-117020210021-7 to ICBFM SB RAS and #AAAA-A19-119031890015-0 to IBP SB RAS.
Предметные рубрики: DNA APTAMER
RNA APTAMER
OBELIN
PURIFICATION
EXPRESSION
SEQUENCES
Аннотация: We report a novel bioluminescent aptasensor, which consists of 2 '-F-RNA aptamer modules joined into a bi-specific aptamer construct. One aptamer module binds the analyte, then after structural rearrangement the second module recruits non-covalently Ca2+-dependent photoprotein obelin from the solution, thus providing a bioluminescent signal. This concept allows using free protein as a reporter, which brings such advantages as no need for aptamer-protein conjugation, a possibility of thermal re-folding of aptamer component with no harm to a protein, and simpler detection protocol. We developed the new 2 '-F-RNA aptamer for obelin, and proposed the strategy for engineering structure-switching bi-modular aptamer constructs which bind the analyte and the obelin in a sequential manner. With the use of hemoglobin as a model analyte, we showed the feasibility of utilizing the aptasensor in a fast and straightforward bioluminescent microplate assay. With a proper design of a secondary structure, this strategy of aptasensor engineering might be further extended to bi-specific aptamer-based bioluminescent sensors for other analytes of interest.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Beregovaya K. A., Myshkina N. M., Chepurnykh, T., V, Kotlobay A. A., Purtov, K., V, Petushkov V. N., Rodionova N. S., Yampolsky, I., V
Заглавие : Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi
Колич.характеристики :4 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-00052P]; President grant for leading scientific schoolsLeading Scientific Schools Program [NSh-2605.2020.4]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2021. - Vol. 496, Is. 1. - С. 14-17. - ISSN 1607-6729, DOI 10.1134/S1607672921010026. - ISSN 1608-3091(eISSN)
Примечания : Cited References:12. - This work was supported by the grant from the Russian Science Foundation no. 16-14-00052P, alanine screening was performed by the President grant for leading scientific schools NSh-2605.2020.4.
Аннотация: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Beregovaya K. A., Myshkina N. M., Chepurnykh T. V., Kotlobay A. A., Purtov K. V., Petushkov V. N., Rodionova N. S., Yampolsky I. V.
Заглавие : Rational Design and Mutagenesis of Fungal Luciferase from Neonothopanus nambi
Место публикации : Doklad. Biochem. Biophys.: Pleiades journals, 2021. - Vol. 496, Is. 1. - С. 14-17. - ISSN 16076729 (ISSN), DOI 10.1134/S1607672921010026
Аннотация: Abstract: The recently described bioluminescent system from fungi has great potential for developing highly efficient tools for biomedical research. Luciferase enzyme is one of the most crucial components of this system. The luciferase from Neonothopanus nambi fungus belongs to the novel still undescribed protein family. The structure data for this protein is almost absent. A detailed study of the N. nambi luciferase properties is necessary for the improvement of analytical methods based on the fungal bioluminescent system. Here we present the positions of key amino acid residues and their effect on enzyme function described using bioinformatic and experimental approaches. These results are useful for further fungal luciferase structure determination. © 2021, Pleiades Publishing, Ltd.
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10.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Bartsev S.I., Okhonin V.A.
Заглавие : Potentialities of theoretical and experimental prediction of life support systems reliability
Колич.характеристики :6 с
Место публикации : LIFE SCIENCES: ARTIFICIAL ECOSYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1999. - Vol. 24: F4 3 and F4 4 Symposia of COSPAR Scientific Commission F Held at 31st COSPAR Scientific Assembly (JUL 14-21, 1996, BIRMINGHAM, ENGLAND), Is. 3. - P407-412. - ISBN 0273-1177, DOI 10.1016/S0273-1177(99)00491-3
Примечания : Cited References: 13
Аннотация: To develop and design Life Support Systems it is necessary to evaluate their reliability. However direct experiments take much time, are very expensive, and therefore are practically impossible. Promising way is to use approximate estimates of reliability, which need essentially fewer amounts of experimental data. Two types of estimates of Life Support System reliability - additive and multiplicative ones are considered in the paper. Additive estimate is based on the assumption that total system failure probability is low and therefore it can be considered as the sum of failure probability of separate units. Additive approach allows obtaining near lower-bounded estimate of failure probability. Multiplicative estimate allows evaluating the possibility of system catastrophe due to simultaneous effect of several factors when each of them separately is not dangerous. Evaluation shows that the possible error of reliability forecast increases with the increasing of number of external factors faster than exponential function. An illustration of the ecological similarity approach as promising tool for providing estimation of full-scale system reliability by means the set of small similar experimental models. (C) 1999 COSPAR. Published by Elsevier Science Ltd.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bartsev S.I., Gitelson J.I., Lisovsky G.M., Mezhevikin V.V., Okhonin V.A.
Заглавие : Perspectives of different type biological life support systems (BLSS) usage in space missions
Место публикации : Acta Astronautica. - 1996. - Vol. 39, Is. 8. - С. 617-622. - ISSN 00945765 (ISSN) , DOI 10.1016/S0094-5765(97)00012-X
Ключевые слова (''Своб.индексиров.''): article--bacterium--comparative study--construction work and architectural phenomena--environmental planning--green alga--human--instrumentation--mathematics--microclimate--moon--plant--quality of life--space flight--standard--weightlessness--algae, green--bacteria--ecological systems, closed--environment design--environment, controlled--facility design and construction--humans--life support systems--mathematics--moon--plants--quality of life--space flight--weightlessness--biology--life support systems (spacecraft)--spreadsheets--biological life support systems (blss)--lunar missions
Аннотация: In the paper an attempt is made to combine three important criteria of LSS comparison: minimum mass, maximum safety and maximum quality of life. Well-known types of BLSS were considered: with higher plant, higher plants and mushrooms, microalgae, and hydrogen-oxidizing bacteria. These BLSSs were compared in terms of "integrated" mass for the case of a vegetarian diet and a "normal" one (with animal proteins and fats). It was shown that the BLSS with higher plants and incineration of wastes becomes the best when the exploitation period is more than 1 yr. The dependence of higher plants' LSS structure on operation time was found. Comparison of BLSSs in terms of integral reliability (this criterion includes mass and quality of life criteria) for a lunar base scenario showed that BLSSs with higher plants are advantageous in reliability and comfort. This comparison was made for achieved level of technology of closing and for perspective one. В© 1997 Elsevier Science Ltd.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhou, Yali, Daver, Henrik, Trapkov, Boris, Wu, Lijie, Wu, Meng, Harpsoe, Kasper, Gentry, Patrick R., Liu, Kaiwen, Larionova, Marina, Liu, Junlin, Chen, N.a., Brauner-Osborne, Hans, Gloriam, David E., Hua, Tian, Liu, Zhi-Jie
Заглавие : Molecular insights into ligand recognition and G protein coupling of the neuromodulatory orphan receptor GPR139
Колич.характеристики :4 с
Коллективы : CAS Strategic Priority Research Program [XDB37030104]; National Science Fund for Distinguished Young ScholarsNational Natural Science Foundation of China (NSFC)National Science Fund for Distinguished Young Scholars [32022038]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31930060, 31870744]; Shanghai Rising-Star Program [20QA1406500]; Lundbeck FoundationLundbeckfonden [R355-2020-949, R163-2013-16327]; Novo Nordisk FoundationNovo Nordisk FoundationNovocure Limited [NNF18OC0031226]; Independent Research Fund Denmark | Natural Sciences [8021-00173B]; Carlsberg FoundationCarlsberg Foundation [CF20-0248]
Место публикации : Cell Res.: SPRINGERNATURE, 2021. - Article in press. - ISSN 1001-0602, DOI 10.1038/s41422-021-00591-w. - ISSN 1748-7838(eISSN)
Примечания : Cited References:16. - This work was supported by the CAS Strategic Priority Research Program XDB37030104 (Z.-J.L.), the National Science Fund for Distinguished Young Scholars 32022038 (T.H.), the National Natural Science Foundation of China grants 31930060 (Z.-J.L.) and 31870744 (T.H.), and the Shanghai Rising-Star Program 20QA1406500 (T. H.), the Lundbeck Foundation R163-2013-16327 (D.E.G.), the Novo Nordisk Foundation NNF18OC0031226 (D.E.G.) and Independent Research Fund Denmark | Natural Sciences 8021-00173B (D.E.G.), the Lundbeck Foundation R355-2020-949 (B.T.) and the Carlsberg Foundation CF20-0248 (H.B.-O.). D.E.G. is a member of the Integrative Structural Biology at the University of Copenhagen (ISBUC). The cryo-EM data were collected at the Bio-Electron Microscopy Facility, ShanghaiTech University, with the assistance of Q.-Q. Sun, D.-D. Liu, Z.-H. Zhang and Y.-H. Liu. We thank the Assay Core, the assistance of F.-F. Zhou and Q.-W. Tan and the Cell Expression, Cloning and Purification Core Facilities of iHuman Institute for their support.
Предметные рубрики: DISCOVERY
PEPTIDES
ALPHA
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kovalev V. S., Grandl W., Manukovsky N. S., Tikhomirov A. A., Bock C.
Заглавие : Modeling a lunar base mushroom farm
Колич.характеристики :6 с
Место публикации : Life Sci. Space Res.: ELSEVIER, 2022. - Vol. 33. - С. 1-6. - ISSN 2214-5524, DOI 10.1016/j.lssr.2021.12.005. - ISSN 2214-5532(eISSN)
Примечания : Cited References:31
Предметные рубрики: SUPPORT
CULTIVATION
Аннотация: To calculate the equivalent system mass of mushrooms, a conceptual configuration of a mushroom farm as part of a bioregenerative life support system on an inhabited lunar base was designed. The mushroom farm consists of two connected modules. Each module is a double-shell rigid pipe-in-pipe aluminum structure. The first module is used to prepare and sterilize the substrate, while the mushrooms are sown and grown in the second module. Planned productivity of the mushroom farm is 28 kg of fresh mushrooms per one process cycle lasting 66 days for 14 consumers. Mushroom production can be increased using additional modules. The calculated equivalent system masses of the mushroom farm and the mushrooms produced therein is 88,432 kg and 31,550 kg per 1 kg of dry mushrooms in one process cycle, respectively. At that, the biggest contributor to the equivalent system mass of mushrooms is the total pressurized volume of the farm - 68%. The results obtained may be a prerequisite for performing trade-off studies between different configurations of mushroom farm and calculating a space diet using the equivalent system mass of mushrooms.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson J.I. U - Okladnikov YuN
Заглавие : Man as a component of a closed ecological life support system
Место публикации : Life support & biosphere science : international journal of earth space. - 1994. - Vol. 1, Is. 2. - С. 73-81. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon dioxide--oxygen--article--blood analysis--blood cell count--breathing--construction work and architectural phenomena--crop--diet--growth, development and aging--human--metabolism--microclimate--physiology--russian federation--space flight--standard--waste management--water supply--blood cell count--blood chemical analysis--carbon dioxide--crops, agricultural--diet--ecological systems, closed--environment, controlled--facility design and construction--humans--life support systems--oxygen--respiration--russia--space flight--waste management--water supply
Аннотация: Material support of all manned space flights so far has been provided from a prestored stock of substances or replenished from the Earth's biosphere. Exploration of space will, however, become real only when man is able to break away from Earth completely, when he will be accompanied by a system providing everything necessary to sustain full-valued life for an unlimited time. The only known system to date meeting this requirement is the Earth's biosphere. To break away from his cradle, as K.E. Tsiolkovsky called Earth, it is necessary to devise a life support system functionally similar to the natural biosphere. This need not be similar in structure to the vast diversity of trophic relationships available on Earth, but requires the solution of a multitude of various problems of an ecological, physiological, engineering and social-psychological nature. Human life-support systems based on biological regeneration of environments in small volumes have been studied at the Institute of Biophysics (Siberian Branch of the Russian Academy of Sciences) over many years. This work has resulted in the design of Bios-3, a biologically-based self-sustained human life support system.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Golubev A. I., Nemtsev I. V., Lukyanenko A. V., Dudaev A. E., Shishatskaya E. I.
Заглавие : Laser processing of polymer films fabricated from phas differing in their monomer composition
Место публикации : Polym.: MDPI AG, 2021. - Vol. 13, Is. 10. - Ст.1553. - ISSN 20734360 (ISSN), DOI 10.3390/polym13101553
Аннотация: The study reports results of using a CO2-laser in continuous wave (3 W; 2 m/s) and quasi-pulsed (13.5 W; 1 m/s) modes to treat films prepared by solvent casting technique from four types of polyhydroxyalkanoates (PHAs), namely poly-3-hydroxybutyrate and three copolymers of 3-hydroxybutyrate: with 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate (each second monomer constituting about 30 mol.%). The PHAs differed in their thermal and molecular weight properties and degree of crystallinity. Pristine films differed in porosity, hydrophilicity, and roughness parameters. The two modes of laser treatment altered these parameters and biocompatibility in diverse ways. Films of P(3HB) had water contact angle and surface energy of 92? and 30.8 mN/m, respectively, and average roughness of 144 nm. The water contact angle of copolymer films decreased to 80–56? and surface energy and roughness increased to 41–57 mN/m and 172–290 nm, respectively. Treatment in either mode resulted in different modifications of the films, depending on their composition and irradiation mode. Laser-treated P(3HB) films exhibited a decrease in water contact angle, which was more considerable after the treatment in the quasi-pulsed mode. Roughness parameters were changed by the treatment in both modes. Continuous wave line-by-line irradiation caused formation of sintered grooves on the film surface, which exhibited some change in water contact angle (76–80? ) and reduced roughness parameters (to 40–45 mN/m) for most films. Treatment in the quasi-pulsed raster mode resulted in the formation of pits with no pronounced sintered regions on the film surface, a more considerably decreased water contact angle (to 67–76? ), and increased roughness of most specimens. Colorimetric assay for assessing cell metabolic activity (MTT) in NIH 3T3 mouse fibroblast culture showed that the number of fibroblasts on the films treated in the continuous wave mode was somewhat lower; treatment in quasi-pulsed radiation mode caused an increase in the number of viable cells by a factor of 1.26 to 1.76, depending on PHA composition. This is an important result, offering an opportunity of targeted surface modification of PHA products aimed at preventing or facilitating cell attachment. © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova, Tatiana G., Golubev, Alexey, I, Nemtsev, Ivan, V, Lukyanenko, Anna, V, Dudaev, Alexey E., Shishatskaya, Ekaterina, I
Заглавие : Laser Processing of Polymer Films Fabricated from PHAs Differing in Their Monomer Composition
Колич.характеристики :24 с
Коллективы : Russian Foundation for Basic Research/Regional State Autonomous Institution "Krasnoyarsk Regional Fund for the Support of Scientific and ScientificTechnical Activities" foundations [19-43-240012]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]
Место публикации : Polymers: MDPI, 2021. - Vol. 13, Is. 10. - Ст.1553. - ISSN 2073-4360(eISSN), DOI 10.3390/polym13101553
Примечания : Cited References:87. - This work was supported by the Russian Foundation for Basic Research/Regional State Autonomous Institution "Krasnoyarsk Regional Fund for the Support of Scientific and ScientificTechnical Activities" foundations under Grant number 19-43-240012 (laser treatment and films properties) and by the State Assignment of the Ministry of Science and Higher Education of the Russian Federation No. FSRZ-2020-0006 (polymer synthesis).
Предметные рубрики: CHEMOMECHANICAL PROPERTIES
PHYSICOCHEMICAL PROPERTIES
SURFACE
Аннотация: The study reports results of using a CO2-laser in continuous wave (3 W; 2 m/s) and quasi-pulsed (13.5 W; 1 m/s) modes to treat films prepared by solvent casting technique from four types of polyhydroxyalkanoates (PHAs), namely poly-3-hydroxybutyrate and three copolymers of 3-hydroxybutyrate: with 4-hydroxybutyrate, 3-hydroxyvalerate, and 3-hydroxyhexanoate (each second monomer constituting about 30 mol.%). The PHAs differed in their thermal and molecular weight properties and degree of crystallinity. Pristine films differed in porosity, hydrophilicity, and roughness parameters. The two modes of laser treatment altered these parameters and biocompatibility in diverse ways. Films of P(3HB) had water contact angle and surface energy of 92 degrees and 30.8 mN/m, respectively, and average roughness of 144 nm. The water contact angle of copolymer films decreased to 80-56 degrees and surface energy and roughness increased to 41-57 mN/m and 172-290 nm, respectively. Treatment in either mode resulted in different modifications of the films, depending on their composition and irradiation mode. Laser-treated P(3HB) films exhibited a decrease in water contact angle, which was more considerable after the treatment in the quasi-pulsed mode. Roughness parameters were changed by the treatment in both modes. Continuous wave line-by-line irradiation caused formation of sintered grooves on the film surface, which exhibited some change in water contact angle (76-80 degrees) and reduced roughness parameters (to 40-45 mN/m) for most films. Treatment in the quasi-pulsed raster mode resulted in the formation of pits with no pronounced sintered regions on the film surface, a more considerably decreased water contact angle (to 67-76 degrees), and increased roughness of most specimens. Colorimetric assay for assessing cell metabolic activity (MTT) in NIH 3T3 mouse fibroblast culture showed that the number of fibroblasts on the films treated in the continuous wave mode was somewhat lower; treatment in quasi-pulsed radiation mode caused an increase in the number of viable cells by a factor of 1.26 to 1.76, depending on PHA composition. This is an important result, offering an opportunity of targeted surface modification of PHA products aimed at preventing or facilitating cell attachment.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Tarasevich A. A., Golubev A. I., Boyandin A. N., Shumilova A. A., Nikolaeva E. D., Shishatskaya E. I.
Заглавие : Laser processing of polymer constructs from poly(3-hydroxybutyrate)
Место публикации : J. Biomater. Sci. Polym. Ed. - 2015. - Vol. 26, Is. 16. - С. 1210-1228. - ISSN 09205063 (ISSN) , DOI 10.1080/09205063.2015.1082810
Ключевые слова (''Своб.индексиров.''): biocompatibility--biopolymers--laser processing--poly(3-hydroxybutyrate)--polymer materials--biocompatibility--biomechanics--biopolymers--bone--cell culture--pulsed lasers--scaffolds (biology)--stem cells--3t3 mouse fibroblasts--bone marrow--bone regeneration--laser process--mesenchymal stem cell--poly-3-hydroxybutyrate--polymer materials--pulsed mode--laser materials processing
Аннотация: CO2 laser radiation was used to process poly(3-hydroxybutyrate) constructs - films and 3D pressed plates. Laser processing increased the biocompatibility of unperforated films treated with moderate uniform radiation, as estimated by the number and degree of adhesion of NIH 3T3 mouse fibroblast cells. The biocompatibility of perforated films modified in the pulsed mode did not change significantly. At the same time, pulsed laser processing of the 3D plates produced perforated scaffolds with improved mechanical properties and high biocompatibility with bone marrow-derived multipotent, mesenchymal stem cells, which show great promise for bone regeneration. © 2015 Taylor & Francis.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Wawrzak Z..., Sandalova T..., Steffens J.J., Basarab G.S., Lundqvist T..., Lindqvist Y..., Jordan D.B.
Заглавие : High-resolution structures of scytalone dehydratase-inhibitor complexes crystallized at physiological pH
Колич.характеристики :15 с
Место публикации : Proteins: WILEY-LISS, 1999. - Vol. 35, Is. 4. - P425-439. - ISSN 0887-3585, DOI 10.1002/(SICI)1097-0134(19990601)35:4425::AID-PROT63.0.CO;2-1
Примечания : Cited References: 33
Предметные рубрики: MAGNAPORTHE-GRISEA
HEMAGGLUTININ
GLYCOPROTEIN
REFINEMENT
MELANIN
DISEASE
SITE
Ключевые слова (''Своб.индексиров.''): structure-based design--enzyme inhibitors--x-ray crystallography--fungicides--melanin biosynthesis
Аннотация: Scytalone dehydratase is a molecular target of inhibitor design efforts aimed at preventing the fungal disease caused by Magnaporthe grisea. A method for cocrystallization of enzyme with inhibitors at neutral pH has produced several crystal structures of enzyme-inhibitor complexes at resolutions ranging from 1.5 to 2.2 Angstrom Four high resolution structures of different enzyme-inhibitor complexes are described. In contrast to the original X-ray structure of the enzyme, the four new structures have well-defined electron density for the loop region comprising residues 115-119 and a different conformation between residues 154 and 160. The structure of the enzyme complex with an aminoquinazoline inhibitor showed that the inhibitor is in a position to form a hydrogen bond with the amide of the Asn131 side chain and with two water molecules in a fashion similar to the salicylamide inhibitor in the original structure, thus confirming design principles. The aminoquinazoline structure also allows for a more confident assignment of donors and accepters in the hydrogen bonding network, The structures of the enzyme complexes with two dichlorocyclopropane carboxamide inhibitors showed the two chlorine atoms nearly in plane with the amide side chain of Asn131. The positions of Phe53 and Phe158 are significantly altered in the new structures in comparison to the two structures obtained from crystals grown at acidic pH, The multiple structures help define the mobility of active site amino acids critical for catalysis and inhibitor binding. Proteins 1999;35:425-439. (C) 1999 Wiley-Liss, Inc.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pilla R. M., Mette E. M., Williamson C. E., Adamovich B. V., Adrian R., Anneville O., Balseiro E., Ban S., Chandra S., Colom-Montero W., Devlin S. P., Dix M. A., Dokulil M. T., Feldsine N. A., Feuchtmayr H., Fogarty N. K., Gaiser E. E., Girdner S. F., Gonzalez M. J., Hambright K. D., Hamilton D. P., Havens K., Hessen D. O., Hetzenauer H., Higgins S. N., Huttula T. H., Huuskonen H., Isles P. D.F., Joehnk K. D., Keller W. B., Klug J., Knoll L. B., Korhonen J., Korovchinsky N. M., Koster O., Kraemer B. M., Leavitt P. R., Leoni B., Lepori F., Lepskaya E. V., Lottig N. R., Luger M. S., Maberly S. C., MacIntyre S., McBride C., McIntyre P., Melles S. J., Modenutti B., Muller-Navarra D. C., Pacholski L., Paterson A. M., Pierson D. C., Pislegina H. V., Plisnier P. -D., Richardson D. C., Rimmer A., Rogora M., Rogozin D. Y., Rusak J. A., Rusanovskaya O. O., Sadro S., Salmaso N., Saros J. E., Sarvala J., Saulnier-Talbot E., Schindler D. E., Shimaraeva S. V., Silow E. A., Sitoki L. M., Sommaruga R., Straile D., Strock K. E., Swain H., Tallant J. M., Thiery W., Timofeyev M. A., Tolomeev A. P., Tominaga K., Vanni M. J., Verburg P., Vinebrooke R. D., Wanzenbock J., Weathers K., Weyhenmeyer G. A., Zadereev E. S., Zhukova T. V.
Заглавие : Global data set of long-term summertime vertical temperature profiles in 153 lakes
Место публикации : Sci. Data: Nature Research, 2021. - Vol. 8, Is. 1. - Ст.200. - ISSN 20524463 (ISSN), DOI 10.1038/s41597-021-00983-y
Аннотация: Climate change and other anthropogenic stressors have led to long-term changes in the thermal structure, including surface temperatures, deepwater temperatures, and vertical thermal gradients, in many lakes around the world. Though many studies highlight warming of surface water temperatures in lakes worldwide, less is known about long-term trends in full vertical thermal structure and deepwater temperatures, which have been changing less consistently in both direction and magnitude. Here, we present a globally-expansive data set of summertime in-situ vertical temperature profiles from 153 lakes, with one time series beginning as early as 1894. We also compiled lake geographic, morphometric, and water quality variables that can influence vertical thermal structure through a variety of potential mechanisms in these lakes. These long-term time series of vertical temperature profiles and corresponding lake characteristics serve as valuable data to help understand changes and drivers of lake thermal structure in a time of rapid global and ecological change. © 2021, The Author(s).
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pilla, Rachel M., Mette, Elizabeth M., Williamson, Craig E., Adamovich, Boris V., Adrian, Rita, Anneville, Orlane, Balseiro, Esteban, Ban, Syuhei, Chandra, Sudeep, Colom-Montero, William, Devlin, Shawn P., Dix, Margaret A., Dokulil, Martin T., Feldsine, Natalie A., Feuchtmayr, Heidrun, Fogarty, Natalie K., Gaiser, Evelyn E., Girdner, Scott F., Gonzalez, Maria J., Hambright, K. David, Hamilton, David P., Havens, Karl, Hessen, Dag O., Hetzenauer, Harald, Higgins, Scott N., Huttula, Timo H., Huuskonen, Hannu, Isles, Peter D. F., Joehnk, Klaus D., Keller, Wendel Bill, Klug, Jen, Knoll, Lesley B., Korhonen, Johanna, Korovchinsky, Nikolai M., Koster, Oliver, Kraemer, Benjamin M., Leavitt, Peter R., Leoni, Barbara, Lepori, Fabio, Lepskaya, Ekaterina V., Lottig, Noah R., Luger, Martin S., Maberly, Stephen C., MacIntyre, Sally, McBride, Chris, McIntyre, Peter, Melles, Stephanie J., Modenutti, Beatriz, Muller-Navarra L., Pacholski, Laura, Paterson, Andrew M., Pierson, Don C., Pislegina, Helen V., Plisnier, Pierre-Denis, Richardson, David C., Rimmer, Alon, Rogora, Michela, Rogozin, Denis Y., Rusak, James A., Rusanovskaya, Olga O., Sadro, Steve, Salmaso, Nico, Saros, Jasmine E., Sarvala, Jouko, Saulnier-Talbot, Emilie, Schindler, Daniel E., Shimaraeva, Svetlana V., Silow, Eugene A., Sitoki, Lewis M., Sommaruga, Ruben, Straile, Dietmar, Strock, Kristin E., Swain, Hilary, Tallant, Jason M., Thiery, Wim, Timofeyev, Maxim A., Tolomeev, Alexander P., Tominaga, Koji, Vanni, Michael J., Verburg, Piet, Vinebrooke, Rolf D., Wanzenbock, Josef, Weathers, Kathleen, Weyhenmeyer, Gesa A., Zadereev, Egor S., Zhukova, Tatyana V.
Заглавие : Global data set of long-term summertime vertical temperature profiles in 153 lakes
Колич.характеристики :12 с
Коллективы : Belarus Republican Foundation for Fundamental Research; AnaEE-France; SILA (Syndicat Mixte du Lac d'Annecy); Castle Lake Environmental Research and Education Program, University of Nevada at Reno; EC project "Response of European Freshwater Lakes [ENV4-CT97-0453]; EC-project "Climate Impacts on European Lakes" [EVK1-CT-2002-00121]; Austrian Climate and Energy Fund [K09ACK00046]; NSFNational Science Foundation (NSF) [DEB 1950170]; Crater Lake National Park Long-Term Limnological Monitoring Program; Ministry of Business, Innovation and Employment: Enhancing the Health and Resilience of New Zealand lakes [UOWX1503]; National Capital Authority; ACT, Australia [GB/NERC/BAS/AEDC/00063]; Belgian Science Policy OfficeBelgian Federal Science Policy Office; North Temperate Lakes LTER NTL-LTER [1440297]; NSERC CanadaNatural Sciences and Engineering Research Council of Canada (NSERC); Canada Research Chairs, Canada Foundation for InnovationCanada Foundation for InnovationCanada Research Chairs; University of Regina; Commissione Internazionale per la protezione delle acque italo-svizzere; Natural Environment Research CouncilUK Research & Innovation (UKRI)Natural Environment Research Council (NERC) [NE/R016429/1]; U.S. NSF Arctic LTER [DEB1637459, LTER_EU_IT_008]; Canton of Zurich [18-44-06201, 20-64-46003]; Russian Ministry of Higher Education and Research [FZZE-2020-0026, FZZE-2020-0023]; National Science Foundation Long Term Research in Environmental Biology program [DEB-1242626]; National Park Service (the Inventory and Monitoring Program); Acadia National Park monitoring program; Gordon and Betty Moore FoundationGordon and Betty Moore Foundation; Andrew Mellon Foundation; US National Science FoundationNational Science Foundation (NSF) [9318452, 9726877, 0235755, 0743192, 1255159]; Institut fur Seenforschung, Langenargen (Internationale Gewasserschutzkommission fur den Bodensee -IGKB); University of Michigan Biological StationUniversity of Michigan System; Belgian Science Policy Office (BELSPO)Belgian Federal Science Policy Office [CD/AR/02 A]; Waikato Regional Council; NIWA; Swedish Research CouncilSwedish Research CouncilEuropean Commission [2017-00635, NSF DEB 1754276]; Lacawac Sanctuary and Biological Field Station; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [19-04-00362 A, 19-05-00428]
Место публикации : Sci. Data: NATURE PORTFOLIO, 2021. - Vol. 8, Is. 1. - Ст.200. - ISSN 2052-4463(eISSN), DOI 10.1038/s41597-021-00983-y
Примечания : Cited References:45. - This work was conceived at the Global Lake Ecological Observatory Network (GLEON), and benefited from continued participation and travel support from GLEON. This manuscript is dedicated to the late Karl Havens and Alon Rimmer, who provided data for this manuscript. Funding and support for this work came from the following sources: the Belarus Republican Foundation for Fundamental Research; the IGB Long-term Ecological Research Programme; SOERE OLA, AnaEE-France, INRA Thonon les Bains, SILA (Syndicat Mixte du Lac d'Annecy), CISALB (Comite Intercommunautaire pour l'Assainissement du Lac du Bourget), and CIPEL (Commission Internationale pour la protection des eaux du Leman); Shiga Prefectural Fisheries Experiment Station (SPFES); Castle Lake Environmental Research and Education Program, University of Nevada at Reno and UC Davis; the Flathead Lake Monitoring program funded through a consortium of state and private funds, and thank the generous citizens of Flathead Lake for their continued support of lake monitoring; the Institute for water ecology, fish biology and lake research and the Institute for Limnology of the Austrian Academy of Sciences (until 2011), and acknowledge the sampling efforts by many individuals over the long period of investigation, especially H. Gassner, M. Luger, H. Ficker, and R. Kurmayer; the EC project "Response of European Freshwater Lakes to Environmental and Climatic Change" (REFLECT, ENV4-CT97-0453), the EC-project "Climate Impacts on European Lakes" (CLIME, EVK1-CT-2002-00121), the project "Risk Analysis of Direct and Indirect Climate effects on deep Austrian Lake Ecosystems" (RADICAL) funded by the Austrian Climate and Energy Fund (No. K09ACK00046) -Austrian Climate Research Programme (ACRP, http://www.klimafonds.gv.at); O. Garcia and E. Bocel for data analysis and management; D. Cabrera, M.W. Dix, G. Ochaeta, S. van Tuylen, M. Orozco, E. Symonds for sampling efforts; NSF grant No. 0947096 to E. Rejmankova, U.S. PeaceCorps and Ministerio de Ambiente y Recursos Naturales of Guatemala; H. Swain, L. Battoe, K. Main, N. Deyrup (Archbold Biological Station), the Florida Lakewatch program, E. Gaiser (Florida International University); the Crater Lake National Park Long-Term Limnological Monitoring Program; the City of Tulsa (R. West and A. Johnson), the Grand River Dam Authority (R. M. Zamor), W.M. Matthews and US ACE (T. Clyde), and the Oklahoma Water Resources Board; Bay of Plenty Regional Council; Ministry of Business, Innovation and Employment: Enhancing the Health and Resilience of New Zealand lakes (UOWX1503); the field and laboratory staff of the South Florida Water Management District for collecting and analyzing the samples; the Norwegian Water Resources and Energy Directorate (NVE), by courtesy of A. S. Kvambekk; the Lake Champlain Long-term Monitoring program (VT DEC and NY DEC); the National Capital Authority, ACT, Australia; Ontario Ministry of Environment, Conservation and Parks; FirstLight Power Resources and Friends of the Lake, especially G. Bollard and R. White; the Finnish Environment Institute SYKE database (Hertta) and S. Mitikka; N. Spinelli and the Lake Wallenpaupack Watershed Management District; Lakes Heywood, Moss, and Sombre: Long-Term Monitoring of Signy Lake Chemistry by BAS 1963-2004. Ref: GB/NERC/BAS/AEDC/00063, and dataset supplied by the Polar Data Centre under Open Government License (c) NERC-BAS, Lake Nkugute: Beadle (1966), CLANIMAE project funded by the Belgian Science Policy Office; Dr. L.; Garibaldi; NSF awards #1418698 and North Temperate Lakes LTER NTL-LTER #1440297; NSERC Canada, Canada Research Chairs, Canada Foundation for Innovation, Province of Saskatchewan, University of Regina, and Queen's University Belfast; Commissione Internazionale per la protezione delle acque italo-svizzere, Ufficio della protezione delle acque e dell'approvvigionamento idrico del Canton Ticino; KamchatNIRO scientists; Natural Environment Research Council award number NE/R016429/1 as part of the UK-SCaPE programme delivering National Capability; U.S. NSF Arctic LTER DEB1637459; Belgian Science Policy (Choltic, Climlake, Climfish); Ontario Ministry of Natural Resources' Harkness Laboratory of Fisheries Research, especially T. Middel; Max-Planck-Institute for Limnology Plon; staff at Erken Laboratory; Mohonk Preserve and D. Smiley; Lake Sunapee Protective Association; KLL database; International Commission for the Protection of Swiss-Italian Waters (CIPAIS) and the LTER (Long Term Ecological Research) Italian network, site "Southern Alpine lakes", LTER_EU_IT_008; staff and students at MECP's Dorset Environmental Science Centre; the LTER (Long-Term Ecological Research) Italian network, site "Southern Alpine lakes", IT08-005-A (http://www.lteritalia.it), with the support of the ARPA Veneto; Prof. L. Chapman, McGill University (Montreal, Quebec, Canada); Amt fur Abfall, Wasser, Energie und Luft (AWEL) of the Canton of Zurich; grants of RSCF project #18-44-06201 and #20-64-46003, of Russian Ministry of Higher Education and Research (projects. FZZE-2020-0026;. FZZE-2020-0023), and of Foundation for support of applied ecological studies "Lake Baikal" (https://baikalfoundation.ru/project/tochka-1/); National Science Foundation Long Term Research in Environmental Biology program (DEB-1242626); the National Park Service (the Inventory and Monitoring Program as well as the Air Resources Division) and Acadia National Park and the Acadia National Park monitoring program; Gordon and Betty Moore Foundation, the Andrew Mellon Foundation, the US National Science Foundation and the Bristol Bay salmon processors; J. Franzoi, G. Larsen, and S. Morales, and the LTSER platform Tyrolean Alps, which belongs to the national and international long-term ecological research network (LTER-Austria, LTER Europe and ILTER); Institut fur Seenforschung, Langenargen (Internationale Gewasserschutzkommission fur den Bodensee -IGKB); University of Michigan Biological Station (A. Schubel) and Cooperative Institute for Great Lakes Research (R. Miller); the Belgian Science Policy Office (BELSPO) is acknowledged for supporting research on Lake Kivu through the research project EAGLES (CD/AR/02 A); US National Science Foundation awards 9318452, 9726877, 0235755, 0743192 and 1255159; West Coast Regional Council, the Bay of Plenty Regional Council, and Waikato Regional Council, and NIWA; D. Schindler (funding and data access) and B. Parker (logistical support and data management); Swedish Infrastructure for Ecosystem Science (SITES) and the Swedish Research Council under the grant no 2017-00635; NSF DEB 1754276 and NSF DEB 1950170, the Ohio Eminent Scholar in Ecosystem Ecology fund, and Lacawac Sanctuary and Biological Field Station; Russian Foundation for Basic Research, grant. 19-04-00362 A and. 19-05-00428.
Предметные рубрики: CLIMATE-CHANGE
THERMAL STRATIFICATION
OXYGEN DEPLETION
FISH
Аннотация: Climate change and other anthropogenic stressors have led to long-term changes in the thermal structure, including surface temperatures, deepwater temperatures, and vertical thermal gradients, in many lakes around the world. Though many studies highlight warming of surface water temperatures in lakes worldwide, less is known about long-term trends in full vertical thermal structure and deepwater temperatures, which have been changing less consistently in both direction and magnitude. Here, we present a globally-expansive data set of summertime in-situ vertical temperature profiles from 153 lakes, with one time series beginning as early as 1894. We also compiled lake geographic, morphometric, and water quality variables that can influence vertical thermal structure through a variety of potential mechanisms in these lakes. These long-term time series of vertical temperature profiles and corresponding lake characteristics serve as valuable data to help understand changes and drivers of lake thermal structure in a time of rapid global and ecological change.
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