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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Voinova O.N., Kalacheva G.S., Grodnitskaya I.D.
Заглавие : The prospects of the use of resorbable polyesters for designing safe pesticides
Место публикации : Doklady Biological Sciences. - 2008. - Vol. 419, Is. 1. - С. 100-103. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496608020099
Ключевые слова (''Своб.индексиров.''): pesticide--polyester--article--chemistry--drug design--metabolism--microbiology--drug design--pesticides--polyesters--soil microbiology
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson J.I., V B., Grigoriev A.I., Lisovsky G.M., Manukovsky N.S., Sinyak Y.u.E., Ushakova S.A.
Заглавие : Biological-physical-chemical aspects of a human life support system for a lunar base
Место публикации : Acta Astronautica. - 1995. - Vol. 37, Is. C. - С. 385-394. - ISSN 00945765 (ISSN)
Ключевые слова (''Своб.индексиров.''): animal--aquaculture--article--biomass--construction work and architectural phenomena--cyprinodontiformes--filtration--growth, development and aging--human--microbiology--microclimate--moon--nutritional value--photoperiodicity--plant--space flight--standard--tilapia--waste management--water management--wheat--animals--aquaculture--biomass--cyprinodontiformes--ecological systems, closed--facility design and construction--filtration--humans--life support systems--moon--nutritive value--photoperiod--plants, edible--space flight--tilapia--triticum--waste management--water microbiology--water purification
Аннотация: To create a life support system based on biological and physical-chemical processes is the optimum solution providing full-valued condidtions for existence and efficient work of people at a lunar base. Long-standing experinece in experimental research or closed ecosystems and their components allows us to suggest a realistic functional structure of the lunar base and to estimate qualitatively its parameters. The original restrictions are as follows: 1) the basic source of energy to support the biological processes has to be the solar radiation; 2) the initial amount of basic biological elelments forming the turnover of substances (C, O, H, P, K, N) has to be delivered from Earth; 3). Moon materials are not to be used in the biological turnover inside the base; 4) the base is to supply the crew fully with atmosphere and water, and with 90% (A scenario) or 40% (B scenario) of food. Experimental data about the plant productivity under the "Moon" rhythm of light and darkness allow us to suggest that the A scenario requires per one human: plant area - 40 m2 irradiated during the lunar day by 250-300 W/m2 PAR producing 1250 g of dry biomass a terrestrial day; a heterotrophic component of "biological incineration" of inedible plant biomass (800 g/day) including the aquaculture of fish to produce animal products and contaminating the environment less than birds and mammals, and the culture of edible mushrooms; a component of physical-chemical correction for the LSS envi ronment including the subsystems of: deep oxidation of organic impurities in the atmosphere and of water, organic wastes of human activity and that biological components (420 g/day) Co2 concentration in "Moon" nights, damping O2 in "Moon" days, etc. The stock of presotred or delivered from Earth substances (food additions, seeds, etc.) to be involved in biological turnover is to be about 50 kg/year per man. Increase of the mass of prestored substances per man up to 220 kg/year would reduce twice the plant area and consumed amount of radiant energy to exclude the components of "biological incineration" and physical-chemical destruction of organic wastes. В© 1995.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Salisbury F.B., Gitelson J.I., Lisovsky G.M.
Заглавие : Bios-3: Siberian experiments in bioregenerative life support
Место публикации : BioScience. - 1997. - Vol. 47, Is. 9. - С. 575-585. - ISSN 00063568 (ISSN)
Ключевые слова (''Своб.индексиров.''): agriculture--chlorella--construction work and architectural phenomena--crop--energy metabolism--evaluation--growth, development and aging--human--metabolism--methodology--microbiology--microclimate--nasa discipline life support systems--non-nasa center--photon--review--russian federation--space flight--nasa discipline life support systems--non-nasa center--agriculture--chlorella--crops, agricultural--ecological systems, closed--energy metabolism--environment, controlled--environmental microbiology--evaluation studies--facility design and construction--humans--life support systems--photons--siberia--space flight--space simulation
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bartsev S.I., Gitelson J.I., Lisovsky G.M., Mezhevikin V.V., Okhonin V.A.
Заглавие : Perspectives of different type biological life support systems (BLSS) usage in space missions
Место публикации : Acta Astronautica. - 1996. - Vol. 39, Is. 8. - С. 617-622. - ISSN 00945765 (ISSN) , DOI 10.1016/S0094-5765(97)00012-X
Ключевые слова (''Своб.индексиров.''): article--bacterium--comparative study--construction work and architectural phenomena--environmental planning--green alga--human--instrumentation--mathematics--microclimate--moon--plant--quality of life--space flight--standard--weightlessness--algae, green--bacteria--ecological systems, closed--environment design--environment, controlled--facility design and construction--humans--life support systems--mathematics--moon--plants--quality of life--space flight--weightlessness--biology--life support systems (spacecraft)--spreadsheets--biological life support systems (blss)--lunar missions
Аннотация: In the paper an attempt is made to combine three important criteria of LSS comparison: minimum mass, maximum safety and maximum quality of life. Well-known types of BLSS were considered: with higher plant, higher plants and mushrooms, microalgae, and hydrogen-oxidizing bacteria. These BLSSs were compared in terms of "integrated" mass for the case of a vegetarian diet and a "normal" one (with animal proteins and fats). It was shown that the BLSS with higher plants and incineration of wastes becomes the best when the exploitation period is more than 1 yr. The dependence of higher plants' LSS structure on operation time was found. Comparison of BLSSs in terms of integral reliability (this criterion includes mass and quality of life criteria) for a lunar base scenario showed that BLSSs with higher plants are advantageous in reliability and comfort. This comparison was made for achieved level of technology of closing and for perspective one. В© 1997 Elsevier Science Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson J.I. U - Okladnikov YuN
Заглавие : Man as a component of a closed ecological life support system
Место публикации : Life support & biosphere science : international journal of earth space. - 1994. - Vol. 1, Is. 2. - С. 73-81. - ISSN 10699422 (ISSN)
Ключевые слова (''Своб.индексиров.''): carbon dioxide--oxygen--article--blood analysis--blood cell count--breathing--construction work and architectural phenomena--crop--diet--growth, development and aging--human--metabolism--microclimate--physiology--russian federation--space flight--standard--waste management--water supply--blood cell count--blood chemical analysis--carbon dioxide--crops, agricultural--diet--ecological systems, closed--environment, controlled--facility design and construction--humans--life support systems--oxygen--respiration--russia--space flight--waste management--water supply
Аннотация: Material support of all manned space flights so far has been provided from a prestored stock of substances or replenished from the Earth's biosphere. Exploration of space will, however, become real only when man is able to break away from Earth completely, when he will be accompanied by a system providing everything necessary to sustain full-valued life for an unlimited time. The only known system to date meeting this requirement is the Earth's biosphere. To break away from his cradle, as K.E. Tsiolkovsky called Earth, it is necessary to devise a life support system functionally similar to the natural biosphere. This need not be similar in structure to the vast diversity of trophic relationships available on Earth, but requires the solution of a multitude of various problems of an ecological, physiological, engineering and social-psychological nature. Human life-support systems based on biological regeneration of environments in small volumes have been studied at the Institute of Biophysics (Siberian Branch of the Russian Academy of Sciences) over many years. This work has resulted in the design of Bios-3, a biologically-based self-sustained human life support system.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson J.I.
Заглавие : Biological life-support systems for Mars mission
Место публикации : Advances in Space Research. - 1992. - Vol. 12, Is. 5. - С. 167-192. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): air conditioning--alcaligenes--article--astronomy--biomass--chlorella--classification--comparative study--equipment design--human--instrumentation--microclimate--plant--solar energy--space flight--waste management--water management--air conditioning--alcaligenes--biomass--chlorella--ecological systems, closed--equipment design--humans--life support systems--mars--plants--solar energy--space flight--spacecraft--waste management--water purification
Аннотация: Mars mission like the Lunar base is the first venture to maintain human life beyond earth biosphere. So far, all manned space missions including the longest ones used stocked reserves and can not be considered egress from biosphere. В© 1991.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kuznetsov A.M., Rodicheva E.K., Medvedeva S.E.
Заглавие : Biotesting of Effluent and River Water by Lyophilized Luminous Bacteria Biotest
Место публикации : Field Analytical Chemistry and Technology. - 1998. - Vol. 2, Is. 5. - С. 267-275. - ISSN 1086900X (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescence--effluent--luminous bacteria biotest--toxicity--water quality
Аннотация: Waters of the Yenisei River, certain rivers and lakes of the Altai Territory, and effluents of some industrial factories in Krasnoyarsk were studied by luminous bacteria biotest Microbiosensor B17 677F. The lyophilized luminous bacteria Photobacterium phosphoreum from the IBSO collection were used to design this biotest. The bioluminescent test is based on bioluminescence quenching resulting from the action of water samples on luminous bacteria. The test results indicated locations and zones of impaired water quality. The heaviest pollution of water in the Yenisei River was recorded in the zones 0-116 km downstream from Krasnoyarsk (Krasnoyarsk and satellite towns). The effluents of most factories were found to be toxic. Underground and surface waters of some areas of the Altai Territory had different toxicity levels; there were deviations from the norm in most water samples taken from the different lakes and rivers. The data from this study show that the luminous bacteria biotest is simple and convenient, and that the results obtained are within acceptable levels of accuracy for the evaluation of the toxicity of effluent and river water. It takes no more than 30 min to do the biotest. It can be used in ecological monitoring like the Microtox toxicity test. В© 1998 John Wiley & Sons, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S.V., Boyandin A.N., Kalacheva G.S., Sinskey A.J.
Заглавие : Degradable Polyhydroxyalkanoates as Herbicide Carriers
Коллективы : Government of the Russian Federation [11.G34.31.0013]
Место публикации : J. Polym. Environ.: SPRINGER/PLENUM PUBLISHERS, 2013. - Vol. 21, Is. 3. - С. 675-682. - 8. - ISSN 1566-2543, DOI 10.1007/s10924-012-0561-z
Примечания : Cited References: 31. - We thank Dr. Christopher Brigham for critical review of the manuscript prior to submission. The study was supported by the project initiated by the Government of the Russian Federation for governmental support of scientific research conducted under the guidance of leading scientists at Russian institutions of higher learning (Agreement No. 11.G34.31.0013).
Предметные рубрики: CONTROLLED-RELEASE
SOIL
BIODEGRADATION
POLYESTERS
PESTICIDE
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
MICROSPHERES
BACTERIA
Ключевые слова (''Своб.индексиров.''): herbicide--haloxyfop-p-methyl--zellek super--sustained-release formulations--polyhydroxyalkanoates--plant growth suppression
Аннотация: The biodegradable polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) has been used to design experimental sustained-release formulations of the herbicide Zellek Super in the form of films and microgranules. The kinetics of polymer degradation and the dynamics of herbicide release show that the rate and extent of herbicide release from the polymer matrix into the soil depends on the geometry of the carrier and the proportion of the pesticide loaded into it (polymer/pesticide mass ratio). Experiments with the creeping bentgrass (Agrostis stolonifera L.) show that the formulations of the herbicide Zellek Super constructed as microgranules and films can be successfully used to suppress the growth of grasses. This study is the first to demonstrate that biodegradable polyhydroxyalkanoates can be used effectively to construct environmentally friendly sustained-release PHA-herbicide systems that can be placed into the soil together with seeds.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Puzyr' A.P., Pozdnyakova I.O., Bondar' V.S.
Заглавие : Design of a luminescent biochip with nanodiamonds and bacterial luciferase
Колич.характеристики :3 с
Место публикации : Phys. Solid State: AMER INST PHYSICS, 2004. - Vol. 46, Is. 4. - С. 761-763. - ISSN 1063-7834, DOI 10.1134/1.1711469
Примечания : Cited References: 10
Аннотация: An "aluminum oxide film-adhesive layer-nanodiamond-luciferase" supramolecular structure is prepared on a flat plate. It is demonstrated that, in this structure, the enzyme retains the catalytic activity. The structure prepared can be treated as a luminescent biochip prototype for use in bioluminescent analysis. (C) 2004 MAIK "Nauka / Interperiodica".
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bezrukikh A., Esimbekova E., Nemtseva E., Kratasyuk V., Shimomura O.
Заглавие : Gelatin and starch as stabilizers of the coupled enzyme system of luminous bacteria NADH:FMN-oxidoreductase-luciferase
Место публикации :. - 2014. - ISSN 16182642 (ISSN) , DOI 10.1007/s00216-014-7987-1
Ключевые слова (''Своб.индексиров.''): bacterial luciferase--bioluminescence--gelatin--nadh:fmn-oxidoreductase--stabilization of enzymes--starch
Аннотация: We have studied the effects of a gel-like environment on the characteristics of enzyme preparations based on the coupled enzyme system of luminous bacteria, NADH:FMN-oxidoreductase-luciferase, to design a stable immobilizing reagent for bioluminescent analysis. Natural polymers, gelatin and starch, were used to create a viscous, structured microenvironment. The stability of the coupled enzyme system to such physical and chemical environmental factors as temperature, pH, and ionic strength in gelatin and starch-containing media was examined. It was shown that both gelatin and starch have a stabilizing effect on the enzymes of luminous bacteria under specific conditions. In particular, the enzymes' activity is increased twofold in the presence of 1 and 5 % of gelatin at 20 °C and 25 °C, respectively (temperatures lower than the gel point). Also, the acceptable pH range of the coupled enzyme system expands into the alkaline region and becomes 6.8-8.1. Stabilization at low ionic strength (0.01-0.06 mol L-1) is observed. At the same time, microenvironments based on either gelatin or starch do not change the enzymes' thermal inactivation rate constants in the temperature range from 25 to 43 °C. Finally, gelatin and starch are suitable for development of a reagent for immobilization of enzymes which would be stable and resistant to physical and chemical environmental conditions. © 2014 Springer-Verlag Berlin Heidelberg.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bezrukikh A..., Esimbekova E..., Nemtseva E..., Kratasyuk V..., Shimomura O...
Заглавие : Gelatin and starch as stabilizers of the coupled enzyme system of luminous bacteria NADH:FMN-oxidoreductase-luciferase
Колич.характеристики :5 с
Коллективы : Government of Russian Federation [11.G34.31.0058]; Russian Academy of Sciences [6.8]; Ministry of Education and Science [1762]; Siberian Federal University [1762]
Место публикации : Anal. Bioanal. Chem.: SPRINGER HEIDELBERG, 2014. - Vol. 406, Is. 23. - С. 5743-5747. - ISSN 1618-2642, DOI 10.1007/s00216-014-7987-1. - ISSN 1618-2650
Примечания : Cited References: 14. - The work was supported by the Program of the Government of Russian Federation "Measures to attract leading scientists to Russian educational institutions" (grant no. 11.G34.31.0058), the Russian Academy of Sciences (program "Molecular and Cell Biology", grant no. 6.8), and the state contract between the Ministry of Education and Science and Siberian Federal University, no. 1762.
Предметные рубрики: IMMOBILIZATION
CHEMISTRY
Ключевые слова (''Своб.индексиров.''): bacterial luciferase--nadh:fmn-oxidoreductase--bioluminescence--stabilization of enzymes--gelatin--starch
Аннотация: We have studied the effects of a gel-like environment on the characteristics of enzyme preparations based on the coupled enzyme system of luminous bacteria, NADH:FMN-oxidoreductase-luciferase, to design a stable immobilizing reagent for bioluminescent analysis. Natural polymers, gelatin and starch, were used to create a viscous, structured microenvironment. The stability of the coupled enzyme system to such physical and chemical environmental factors as temperature, pH, and ionic strength in gelatin and starch-containing media was examined. It was shown that both gelatin and starch have a stabilizing effect on the enzymes of luminous bacteria under specific conditions. In particular, the enzymes' activity is increased twofold in the presence of 1 and 5 % of gelatin at 20 A degrees C and 25 A degrees C, respectively (temperatures lower than the gel point). Also, the acceptable pH range of the coupled enzyme system expands into the alkaline region and becomes 6.8-8.1. Stabilization at low ionic strength (0.01-0.06 mol L-1) is observed. At the same time, microenvironments based on either gelatin or starch do not change the enzymes' thermal inactivation rate constants in the temperature range from 25 to 43 A degrees C. Finally, gelatin and starch are suitable for development of a reagent for immobilization of enzymes which would be stable and resistant to physical and chemical environmental conditions.
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12.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : BARTSEV S.I., MEZHEVIKIN V.V., OKHONIN V.A.
Заглавие : BIOS-4 AS AN EMBODIMENT OF CELSS DEVELOPMENT CONCEPTION
Колич.характеристики :4 с
Место публикации : PHYSICAL, CHEMICAL, BIOCHEMICAL AND BIOLOGICAL TECHNIQUES AND PROCESSES. Ser. ADVANCES IN SPACE RESEARCH-SERIES: PERGAMON PRESS LTD, 1996. - Vol. 18: F2.2, F2.3, F2.5, F4.3, F4.4, F4.9, F4.10 Meetings of COSPAR Scientific Commission F, at the 13th COSPAR Scientific Assembly (JUL 11-21, 1994, HAMBURG, GERMANY), Is. 01.02.2013. - P201-204. - ISBN 0273-1177, DOI 10.1016/0273-1177(95)00809-S. - ISBN 0-08-042664-6
Примечания : Cited References: 3
Аннотация: Any attempt to create LSS for practical applications must take into account the possibility of catastrophic consequences if the problem of LSS reliability and stability is not solved. An integrated conception of CELSS studies development as a possible way to increase its reliability is considered. The BIOS-4 facility project is developed in the context of the conception. Three principles of highly effective experimental CELSS facility design are proposed. Some details of BIOS-4 design and its exploitation features are presented.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Wawrzak Z..., Sandalova T..., Steffens J.J., Basarab G.S., Lundqvist T..., Lindqvist Y..., Jordan D.B.
Заглавие : High-resolution structures of scytalone dehydratase-inhibitor complexes crystallized at physiological pH
Колич.характеристики :15 с
Место публикации : Proteins: WILEY-LISS, 1999. - Vol. 35, Is. 4. - P425-439. - ISSN 0887-3585, DOI 10.1002/(SICI)1097-0134(19990601)35:4425::AID-PROT63.0.CO;2-1
Примечания : Cited References: 33
Предметные рубрики: MAGNAPORTHE-GRISEA
HEMAGGLUTININ
GLYCOPROTEIN
REFINEMENT
MELANIN
DISEASE
SITE
Ключевые слова (''Своб.индексиров.''): structure-based design--enzyme inhibitors--x-ray crystallography--fungicides--melanin biosynthesis
Аннотация: Scytalone dehydratase is a molecular target of inhibitor design efforts aimed at preventing the fungal disease caused by Magnaporthe grisea. A method for cocrystallization of enzyme with inhibitors at neutral pH has produced several crystal structures of enzyme-inhibitor complexes at resolutions ranging from 1.5 to 2.2 Angstrom Four high resolution structures of different enzyme-inhibitor complexes are described. In contrast to the original X-ray structure of the enzyme, the four new structures have well-defined electron density for the loop region comprising residues 115-119 and a different conformation between residues 154 and 160. The structure of the enzyme complex with an aminoquinazoline inhibitor showed that the inhibitor is in a position to form a hydrogen bond with the amide of the Asn131 side chain and with two water molecules in a fashion similar to the salicylamide inhibitor in the original structure, thus confirming design principles. The aminoquinazoline structure also allows for a more confident assignment of donors and accepters in the hydrogen bonding network, The structures of the enzyme complexes with two dichlorocyclopropane carboxamide inhibitors showed the two chlorine atoms nearly in plane with the amide side chain of Asn131. The positions of Phe53 and Phe158 are significantly altered in the new structures in comparison to the two structures obtained from crystals grown at acidic pH, The multiple structures help define the mobility of active site amino acids critical for catalysis and inhibitor binding. Proteins 1999;35:425-439. (C) 1999 Wiley-Liss, Inc.
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14.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Bartsev S.I., Okhonin V.A.
Заглавие : Potentialities of theoretical and experimental prediction of life support systems reliability
Колич.характеристики :6 с
Место публикации : LIFE SCIENCES: ARTIFICIAL ECOSYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1999. - Vol. 24: F4 3 and F4 4 Symposia of COSPAR Scientific Commission F Held at 31st COSPAR Scientific Assembly (JUL 14-21, 1996, BIRMINGHAM, ENGLAND), Is. 3. - P407-412. - ISBN 0273-1177, DOI 10.1016/S0273-1177(99)00491-3
Примечания : Cited References: 13
Аннотация: To develop and design Life Support Systems it is necessary to evaluate their reliability. However direct experiments take much time, are very expensive, and therefore are practically impossible. Promising way is to use approximate estimates of reliability, which need essentially fewer amounts of experimental data. Two types of estimates of Life Support System reliability - additive and multiplicative ones are considered in the paper. Additive estimate is based on the assumption that total system failure probability is low and therefore it can be considered as the sum of failure probability of separate units. Additive approach allows obtaining near lower-bounded estimate of failure probability. Multiplicative estimate allows evaluating the possibility of system catastrophe due to simultaneous effect of several factors when each of them separately is not dangerous. Evaluation shows that the possible error of reliability forecast increases with the increasing of number of external factors faster than exponential function. An illustration of the ecological similarity approach as promising tool for providing estimation of full-scale system reliability by means the set of small similar experimental models. (C) 1999 COSPAR. Published by Elsevier Science Ltd.
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15.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Bartsev S.I., Mezhevikin V.V., Okhonin V.A.
Заглавие : Systematic approach to life support system analyses and integration
Колич.характеристики :10 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P1823-1832. - ISBN 0273-1177, DOI 10.1016/S0273-1177(03)00081-4
Примечания : Cited References: 25
Предметные рубрики: RELIABILITY
SPACE
Аннотация: This paper is devoted to the consideration of possible viewpoint on CELSS development and design. If the aim to create practically applicable CELSS is accepted then the task to optimize the process of CELSS research and development in terms of minimum cost, hours, maximum applicability, scientific contribution, etc. becomes actual. Requirements of applicability and scientific significance are synergetic since understanding of general properties of CELSS gives an ability to create CELSS for different applications. To accomplish the task three main groups of parameters have to be optimized: i) configuration and operating parameters of developing CELSS itself, ii) organizational management of research and development of CELSS; iii) features of an area where CELSS is planned to be used (space missions, terrestrial applications, or biosphere investigation) and where requirements to CELSS characteristic come from. Given paper is a brief review presented some attempts to arrange mentioned above into some set of formalized and interacting criteria, and some progression of research stages derived from these criteria. (C) 2003 Published by Elsevier Science Ltd on behalf of COSPAR.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogil'naya O.A., Krivomazova E.S., Kargatova T.V., Lobova T.I., Popova L.Y.
Заглавие : Formation of structured communities by natural and transgenic naphthalene-degrading bacteria
Колич.характеристики :6 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 2005. - Vol. 41, Is. 1. - P63-68. - ISSN 0003-6838, DOI 10.1007/s10438-005-0012-x
Примечания : Cited References: 18
Предметные рубрики: GENETICALLY-ENGINEERED MICROORGANISM
POLYCYCLIC AROMATIC-HYDROCARBONS
BIOFILM FORMATION
DEGRADATION
Аннотация: This study concerns the formation of structured communities by monocultures and binary associations of Pseudomonas fluorescens transgenic strains and natural heterotrophic bacterial species in naphthalene-containing media with various osmotic pressures. It was shown that cells of P. fluorescens strain 5RL, harboring a recombinant construct in the chromosome, were more resistant to the combined action of the stress factors under study than P. fluorescens 82/pUTK21, harboring a recombinant construct within a plasmid. Natural P. fluorescens 1 strains, particularly Vibrio sp. 14, were more viable at high osmotic pressures and naphthalene concentrations. Experiments with the combined introduction of transgenic and natural bacterial strains at high osmotic pressures demonstrated the stable coexistence of bacterial associations in biofilms, independent of naphthalene concentration. Strains considered for introduction into the environment for bioremediation should be assessed with regard to their susceptibility to the combined effect of anthropogenic and natural stress factors. The design of bacterial associations for the same purpose should take into account the effect of factors important for their survival in polluted areas.
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17.

Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Esimbekova, Elena, Kratasyuk, Valentina, Shimomura, Osamu
Заглавие : Application of Enzyme Bioluminescence in Ecology
Колич.характеристики :43 с
Место публикации : Adv. Biochem. Eng. Biotechnol.: SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - С. 67-109. - (Advances in Biochemical Engineering-Biotechnology). - , DOI 10.1007/978-3-662-43385-0_3
Примечания : Cited References:85
Предметные рубрики: BACTERIAL LUCIFERASE
IN-VITRO
PYRETHROID INSECTICIDES
FRESH-WATER
Ключевые слова (''Своб.индексиров.''): bioluminescence--ecological monitoring--enzymatic assay--immobilization--integral water toxicity--luciferase
Аннотация: This review examines the general principles of bioluminescent enzymatic toxicity bioassays and describes the applications of these methods and the implementation in commercial biosensors. Bioluminescent enzyme system technology (BEST) has been proposed in the bacterial coupled enzyme system, wherein NADH: FMN-oxidoreductase-luciferase substitutes for living organisms. BEST was introduced to facilitate and accelerate the development of cost-competitive enzymatic systems for use in biosensors for medical, environmental, and industrial applications. For widespread use of BEST, the multicomponent reagent "Enzymolum'' has been developed, which contains the bacterial luciferase, NADH: FMN-oxidoreductase, and their substrates, co-immobilized in starch or gelatin gel. Enzymolum is the central part of Portable Laboratory for Toxicity Detection (PLTD), which consists of a biodetector module, a sampling module, a sample preparation module, and a reagent module. PLTD instantly signals chemical-biological hazards and allows us to detect a wide range of toxic substances. Enzymolum can be integrated as a biological module into the portable biodetector-biosensor originally constructed for personal use. Based on the example of Enzymolum and the algorithm for creating new enzyme biotests with tailored characteristics, a new approach was demonstrated in biotechnological design and construction. The examples of biotechnological design of various bioluminescent methods for ecological monitoring were provided. Possible applications of enzyme bioassays are seen in the examples for medical diagnostics, assessment of the effect of physical load on sportsmen, analysis of food additives, and in practical courses for higher educational institutions and schools. The advantages of enzymatic assays are their rapidity (the period of time required does not exceed 3-5 min), high sensitivity, simplicity and safety of procedure, and possibility of automation of ecological monitoring; the required luminometer is easily available.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Puzyr A.P., Burov A.E., Medvedeva S.E., Rodicheva E.K., Kobzeva T.V., Melnikov A.R., Karogodina T.Y., Zikirin S.B., Stass D.V., Molin, Yu. N., Gitelson J.I.
Заглавие : Effect of ionizing radiation on the luminescence of mycelium of luminous fungus Neonothopanus nambi
Колич.характеристики :4 с
Коллективы : Support Program for Interdisciplinary Projects of the Siberian Branch of the Russian Academy of Sciences [71], Program of the President of the Russian Federation for Support of Leading Scientific Schools [NSh-5744.2014.3]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 460, Is. 1. - С. 30-33. - ISSN 1607-6729, DOI 10.1134/S1607672915010093. - ISSN 1608-3091(eISSN)
Примечания : Cited References:15. - This work was supported in part by the Support Program for Interdisciplinary Projects of the Siberian Branch of the Russian Academy of Sciences (project no. 71) and the Program of the President of the Russian Federation for Support of Leading Scientific Schools (project no. NSh-5744.2014.3).
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Esimbekova, E. N., Lonshakova-Mukina, V. I., Bezrukikh, A. E., Kratasyuk, V. A.
Заглавие : Design of multicomponent reagents for enzymatic assays
Колич.характеристики :4 с
Коллективы : Program of the Russian Academy of Sciences "Molecular and Cell Biology" [6.8], Ministry of Education and Science of the Russian Federation; Siberian Federal University [1762]
Место публикации : Dokl. Biochem. Biophys.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2015. - Vol. 461, Is. 1. - С. 102-105. - ISSN 1607-6729, DOI 10.1134/S1607672915020106. - ISSN 1608-3091(eISSN)
Примечания : Cited References:8. - The work was supported by the Program of the Russian Academy of Sciences "Molecular and Cell Biology" (project no. 6.8) as well as within the state contract between the Ministry of Education and Science of the Russian Federation and Siberian Federal University (project 1762).
Предметные рубрики: BIOLUMINESCENT
WATER
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T. G., Tarasevich A. A., Golubev A. I., Boyandin A. N., Shumilova A. A., Nikolaeva E. D., Shishatskaya E. I.
Заглавие : Laser processing of polymer constructs from poly(3-hydroxybutyrate)
Место публикации : J. Biomater. Sci. Polym. Ed. - 2015. - Vol. 26, Is. 16. - С. 1210-1228. - ISSN 09205063 (ISSN) , DOI 10.1080/09205063.2015.1082810
Ключевые слова (''Своб.индексиров.''): biocompatibility--biopolymers--laser processing--poly(3-hydroxybutyrate)--polymer materials--biocompatibility--biomechanics--biopolymers--bone--cell culture--pulsed lasers--scaffolds (biology)--stem cells--3t3 mouse fibroblasts--bone marrow--bone regeneration--laser process--mesenchymal stem cell--poly-3-hydroxybutyrate--polymer materials--pulsed mode--laser materials processing
Аннотация: CO2 laser radiation was used to process poly(3-hydroxybutyrate) constructs - films and 3D pressed plates. Laser processing increased the biocompatibility of unperforated films treated with moderate uniform radiation, as estimated by the number and degree of adhesion of NIH 3T3 mouse fibroblast cells. The biocompatibility of perforated films modified in the pulsed mode did not change significantly. At the same time, pulsed laser processing of the 3D plates produced perforated scaffolds with improved mechanical properties and high biocompatibility with bone marrow-derived multipotent, mesenchymal stem cells, which show great promise for bone regeneration. © 2015 Taylor & Francis.
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