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Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Esimbekova, Elena, Kratasyuk, Valentina, Shimomura, Osamu
Заглавие : Application of Enzyme Bioluminescence in Ecology
Колич.характеристики :43 с
Место публикации : Adv. Biochem. Eng. Biotechnol.: SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - С. 67-109. - (Advances in Biochemical Engineering-Biotechnology). - , DOI 10.1007/978-3-662-43385-0_3
Примечания : Cited References:85
Предметные рубрики: BACTERIAL LUCIFERASE
IN-VITRO
PYRETHROID INSECTICIDES
FRESH-WATER
Ключевые слова (''Своб.индексиров.''): bioluminescence--ecological monitoring--enzymatic assay--immobilization--integral water toxicity--luciferase
Аннотация: This review examines the general principles of bioluminescent enzymatic toxicity bioassays and describes the applications of these methods and the implementation in commercial biosensors. Bioluminescent enzyme system technology (BEST) has been proposed in the bacterial coupled enzyme system, wherein NADH: FMN-oxidoreductase-luciferase substitutes for living organisms. BEST was introduced to facilitate and accelerate the development of cost-competitive enzymatic systems for use in biosensors for medical, environmental, and industrial applications. For widespread use of BEST, the multicomponent reagent "Enzymolum'' has been developed, which contains the bacterial luciferase, NADH: FMN-oxidoreductase, and their substrates, co-immobilized in starch or gelatin gel. Enzymolum is the central part of Portable Laboratory for Toxicity Detection (PLTD), which consists of a biodetector module, a sampling module, a sample preparation module, and a reagent module. PLTD instantly signals chemical-biological hazards and allows us to detect a wide range of toxic substances. Enzymolum can be integrated as a biological module into the portable biodetector-biosensor originally constructed for personal use. Based on the example of Enzymolum and the algorithm for creating new enzyme biotests with tailored characteristics, a new approach was demonstrated in biotechnological design and construction. The examples of biotechnological design of various bioluminescent methods for ecological monitoring were provided. Possible applications of enzyme bioassays are seen in the examples for medical diagnostics, assessment of the effect of physical load on sportsmen, analysis of food additives, and in practical courses for higher educational institutions and schools. The advantages of enzymatic assays are their rapidity (the period of time required does not exceed 3-5 min), high sensitivity, simplicity and safety of procedure, and possibility of automation of ecological monitoring; the required luminometer is easily available.
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Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Frank, Ludmila A., Krasitskaya, Vasilisa V.
Заглавие : Application of Enzyme Bioluminescence for Medical Diagnostics
Колич.характеристики :23 с
Место публикации : Adv. Biochem. Eng. Biotechnol.: SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - С. 175-197. - (Advances in Biochemical Engineering-Biotechnology). - , DOI 10.1007/978-3-662-43385-0_6
Примечания : Cited References:63
Предметные рубрики: RESONANCE ENERGY-TRANSFER
POLYMERASE-CHAIN-REACTION
LUCIFERASE
Ключевые слова (''Своб.индексиров.''): bioluminescence--ca2+-regulated photoprotein--diagnostics--immunoassay--luciferase--nucleic acid hybridization assay
Аннотация: Nowadays luciferases are effectively used as analytical instruments in a great variety of research fields. Of special interest are the studies dealing with elaboration of novel analytical systems for the purposes of medical diagnostics. The ever-expanding spectrum of clinically important analytes accounts for the increasing demand for new techniques for their detection. In this chapter we have made an attempt to summarize the results on applications of luciferases as reporters in binding assays including immunoassay, nucleic acid hybridization assay, and so on. The data over the last 15 years have been analyzed and clearly show that luciferase-based assays, due to extremely high sensitivity, low cost, and the lack of need for skilled personnel, hold much promise for clinical diagnostics.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk, Valentina A., Stepanova, Lyudmila, V, Ranjan, Rajeev, Sutormin, Oleg S., Pande, Shubhra, Zhukova, Galina, V, Miller, Olga M., Maznyak, Natalya, V, Kolenchukova, Oksana A.
Заглавие : A noninvasive and qualitative bioluminescent assay for express diagnostics of athletes' responses to physical exertion
Колич.характеристики :7 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]; Krasnoyarsk Regional Foundation of Science [KF-537]
Место публикации : Luminescence: WILEY, 2020. - Article in press. - ISSN 1522-7235, DOI 10.1002/bio.3954. - ISSN 1522-7243(eISSN)
Примечания : Cited References:33. - The Ministry of Science and Higher Education of the Russian Federation, Grant/Award Number: FSRZ-2020-0006; Krasnoyarsk Regional Foundation of Science, Grant/Award Number: KF-537
Предметные рубрики: SALIVARY BIOMARKERS
EXERCISE
Аннотация: Upcoming professional sports authorities seek rapid noninvasive biosensing tools for regular monitoring of athletes' physiological states. The analysis of saliva through luminescence-based biosensors has been perceived as a suitable candidate for such purposes. The present study reports a qualitative bioluminescence assay based on a coupled enzyme system that consists of bacterial luciferase (BLuc) and nicotinamide adenine dinucleotide (NADH):flavin mononucleotide (FMN) oxidoreductase (Red), BLuc-Red, for the express diagnostics of athletes' stress levels before and after physical exertion. The volunteers who participated in the study were grouped as freestyle wrestlers and students who adapted to different levels of physical activities. Under physical exertion modelling conditions, the influence of participant saliva on BLuc-Red catalyzed light emission was investigated. Results showed a significant increase in residual luminescence (I-exp, mean maximum bioluminescence intensity of the experimental measurement (I-exp); I-c, luminescence intensity in control; I-exp/I-c, %) values for participants in the wrestler group while a decrease in the student group (P 0.05). Such contrasting residual luminescence values in both groups were found to be dependent on the catalase activity of saliva. The proposed bioluminescence assay can be utilized as a potential nonspecific biosensing tool for determining the physical state of athletes under high loads.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shikhov V. N., Nesterenko T. V., Tikhomirov A. A.
Заглавие : Effect of light intensity on chlorophyll fluorescence in wheat leaves: Application of PAM-fluorometry
Место публикации : Russ. J. Plant Physiol. - 2016. - Vol. 63, Is. 3. - С. 417-422. - ISSN 10214437 (ISSN) , DOI 10.1134/S1021443716030134
Ключевые слова (''Своб.индексиров.''): actinic light intensity--induction of chlorophyll fluorescence--leaf ontogeny--triticum aestivum
Аннотация: Application of pulse-amplitude-modulation (PAM) fluorometers for measuring slow stages of chlorophyll fluorescence induction (CFI) is considered. With an example of Triticum aestuvum L. plants grown under continuous illumination at a photon flux density of 600 μmol/(m2 s) photosynthetically active radiation (PAR), the CFI curves were analyzed with leaves of various ages as a function of actinic light intensity. The fluorometer PAM-2100 was applied for measurements of CFI curves. The characteristic peaks of CFI curves in wheat leaves were most conspicuous and had the largest amplitudes at 600–800 μmol/(m2 s) PAR, which corresponds to the middle range of actinic light intensities employed in PAM-2100 fluorometers. In plants exposed to favorable and stressful conditions, the developmental stages may proceed at different rates; thus, the comparison of fluorescence parameters for leaves of equal calendar age but having different physiological states may provide ambiguous data. Therefore, the feasibility of recording CFI curves of different types is quite important for rapid diagnostics of the age and state of plant leaves, as well as for adequate physiological conclusions. © 2016, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Baron A.V., Puzyr A.P., Baron I.I., Bondar V.S.
Заглавие : Effects of Modified Detonation Nanodiamonds on the Biochemical Composition of Human Blood
Колич.характеристики :4 с
Место публикации : Bull. Exp. Biol. Med.: SPRINGER, 2013. - Vol. 154, Is. 6. - С. 781-784. - ISSN 0007-4888, DOI 10.1007/s10517-013-2055-y
Примечания : Cited References: 14
Предметные рубрики: PARTICLES
Ключевые слова (''Своб.индексиров.''): modified nanodiamonds--blood serum
Аннотация: In vitro experiments showed that protein and non-protein components of human blood serum could be absorbed on the surface of modified nanodiamonds obtained by detonation synthesis. The prospects of using nanodiamond as a new absorbent for hemodialysis, plasmapheresis, and laboratory diagnostics are discussed.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shevyrnogov A.P., Sidko A.F.
Заглавие : Remote diagnostics of vegetation covers by spectral brightness of plants
Место публикации : Advances in Space Research. - 1995. - Vol. 16, Is. 10. - С. 185-188. - ISSN 02731177 (ISSN)
Ключевые слова (''Своб.индексиров.''): plant--spectral brightness--vegetation
Аннотация: Optical remote methods are able to solve numerous scientific and practical problems associated with development of ecological monitoring systems, monitoring of plant cenoses' condition, forests and agricultural crops, in particular. Investigation of vegetation covers (VC) by optical remote methods is based on comprehensive examination and analysis of interaction mechanisms of incident and reflected solar light with plants, their dependence on geometric and pigment structure. В© 1995.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Anishchenko O.V., Komakov V.I., Gladyshev M.I.
Заглавие : Research of the meteorological factors influence on the fluorescence measurements of subsurface phytoplankton in blooming reservoir
Место публикации : Doklady Akademii Nauk. - 2004. - Vol. 397, Is. 1. - С. 124-127. - ISSN 08695652 (ISSN)
Ключевые слова (''Своб.индексиров.''): bacteria--fluorescence--meteorological problems--precipitation (meteorology)--reservoirs (water)--solar radiation--wind effects--meteorological effects--algae
Аннотация: The reaction of algae and cyano-bacteria photosynthetic apparatus on atmospheric sediments, incident solar radiation and wind velocity was estimated by measuring chlorophyll fluorescence using three-beam fluorescent diagnostics. As shown, wind in the spring positively affects the chlorophyll concentration but causes negative effect on phytoplankton photosynthetic activity in other seasons. Under conditions of high-intensity solar irradiation confervoid and colonial cyanobacteria, possessing gas vacuoles, have an advantage over plankton eukaryotic algae. Other response differences weren't observed.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Frank L.A., Petunin A.I., Vysotski E.S.
Заглавие : Bioluminescent immunoassay of thyrotropin and thyroxine using obelin as a label
Колич.характеристики :7 с
Место публикации : Anal. Biochem.: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2004. - Vol. 325, Is. 2. - С. 240-246. - ISSN 0003-2697, DOI 10.1016/j.ab.2003.11.003
Примечания : Cited References: 16
Предметные рубрики: AEQUORIN
PURIFICATION
PROTEIN
CDNA
Ключевые слова (''Своб.индексиров.''): obelin--human thyrotropin--thyroxine--immunoassay--bioluminescence
Аннотация: Solid-phase bioluminescent immunoassay of thyroid hormones, human thyrotropin (hTSH) and two forms of thyroxine (T4), whose determinations are vitally important for diagnostics of thyroid diseases and the efficiency of treatment, is described. The recombinant obelin, a Ca2+-regulated photoprotein originally derived from the luminous marine hydroid Obelia longissima, is employed as a bioluminescent label. To produce obelin conjugates with anti-hTSH, anti-T4 immunoglobulins (IgG), and T4, additional SH groups are introduced into the obelin molecule using Traut's reagent (2-iminothiolane) and then obelin possessing extra SH groups is conjugated with succinimidyl 4-(N-maleimidomethyl)-cyclohexane-1-carboxylate-activated IgGs or T4. The total yield of obelin conjugates determined by luminescent activity is 60-65% after all chemical and purification procedures. The obtained conjugates are stable to lyophilization and in solution for at least 9 months at 4 degreesC, with loss of activity not exceeding 10%. The application of obelin conjugates for determination of the hTSH, total T4, and free T4 in standard, control, and patient sera displays high sensitivity and reproducibility of results. The results of bioluminescent immunoassays are closely comparable to those obtained by the radioimmunoassay method (R = 0.95-0.99). (C) 2003 Elsevier Inc. All rights reserved.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : SHEVYRNOGOV A.P., SIDKO A.F.
Заглавие : REMOTE DIAGNOSTICS OF VEGETATION COVERS BY SPECTRAL BRIGHTNESS OF PLANTS
Место публикации : SATELLITE MONITORING OF THE EARTH'S SURFACE AND ATMOSPHERE. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1995. - Vol. 16: A1 Symposium of COSPAR Scientific Commission A on Satellite Monitoring of the Earths Surface and Atmosphere, at the 13th COSPAR Scientific Assembly (JUL 11-21, 1994, HAMBURG, GERMANY), Is. 10. - С. 185-188. - 4. - ISBN 0273-1177, DOI 10.1016/0273-1177(95)00401-Y. - ISBN 0-08-042635-2
Примечания : Cited References: 5
Аннотация: Optical remote methods are able to solve numerous scientific and practical problems associated with development of ecological monitoring systems, monitoring of plant cenoses' condition, forests and agricultural crops, in particular. Investigation of vegetation covers (VC) by optical remote methods is based on comprehensive examination and analysis of interaction mechanisms of incident and reflected solar light with plants, their dependence on geometric and pigment structure.
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