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Вид документа : Статья из журнала
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Автор(ы) : Esimbekova E. N., Asanova A. A., Deeva A. A., Kratasyuk V. A.
Заглавие : Inhibition effect of food preservatives on endoproteinases
Место публикации : Food Chem.: Elsevier Ltd, 2017. - Vol. 235. - С. 294-297. - ISSN 03088146 (ISSN) , DOI 10.1016/j.foodchem.2017.05.059
Ключевые слова (''Своб.индексиров.''): endoproteinases--food additives--pancreatic disease--pancreatic enzymes--benzoic acid--enzyme activity--enzymes--food additives--food preservatives--potassium sorbate--sodium--acceptable daily intakes--decay constants--endoproteinases--human metabolisms--inhibition effect--light intensity--protein digestion--sodium benzoate--sorbic acid
Аннотация: The present manuscript proposes a novel approach to assess the impact of food additives on human metabolism by analysing their effect on biomarker enzyme activity. Alterations in the activity of pancreatic enzymes, such as chymotrypsin and trypsin, which are affected by the most common food preservatives, sodium benzoate (E211), potassium sorbate (E202) and sorbic acid (E200), have been evaluated. The proteinase activity was analysed with a bioluminescent method using the light intensity decay constant. Our study revealed that the preservatives reduce proteinase activity by 50% (EC50) at a much lower concentration than their acceptable daily intake (ADI). Thus, sodium benzoate and sorbic acid have an inhibition effect on chymotrypsin at concentrations 14 times lower and 70 times lower than their ADI and this increases with exposure time. Food preservative consumption impacts negatively on protein digestion, which is especially dangerous for patients with pancreatitis. © 2017 Elsevier Ltd
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