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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Tikhomirov A. A., Ushakova S. A., Tikhomirova N. A., Trifonov S. V., Kalacheva G. S.
Заглавие : Converting gaseous pollutants toxic to plants and humans into environmentally friendly compounds in artificial ecosystems
Место публикации : IOP Conference Series: Materials Science and Engineering: Institute of Physics Publishing, 2018. - Vol. 450: 9th International Multidisciplinary Scientific and Research Conference on Modern Issues in Science and Technology Workshop in Advanced Technologies in Aerospace, Mechanical and Automation Engineering, MISTAerospace 2018 (20 October 2018 through 28 October 2018, ) Conference code: 143027, Is. 6. - , DOI 10.1088/1757-899X/450/6/062004
Ключевые слова (''Своб.индексиров.''): ecosystems--environmental management--fog--artificial ecosystems--experimental conditions--gaseous pollutants--human waste--liquid products--plant growth--toxic effect--wheat plants--pollution
Аннотация: The present study describes detection of potential gaseous pollutants that can produce a toxic effect on plants and humans in the system with wheat plants cultivated on solutions containing liquid products of mineralization of human waste and fish waste. These gaseous pollutants do not inhibit plant growth and development under the experimental conditions, but they may accumulate in closed ecosystems functioning for extended periods of time. Ways to convert gaseous pollutants into environmentally friendly compounds have been proposed. © 2018 Institute of Physics Publishing. All rights reserved.
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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Tikhomirov A. A., Trifonov S. V., Morozov E. A.
Заглавие : Developing the technology of physicochemical processing of organic waste in closed life support systems for space applications
Место публикации : IOP Conference Series: Materials Science and Engineering: Institute of Physics Publishing, 2018. - Vol. 450: 9th International Multidisciplinary Scientific and Research Conference on Modern Issues in Science and Technology Workshop in Advanced Technologies in Aerospace, Mechanical and Automation Engineering, MISTAerospace 2018 (20 October 2018 through 28 October 2018, ) Conference code: 143027, Is. 6. - , DOI 10.1088/1757-899X/450/6/062017
Ключевые слова (''Своб.индексиров.''): electric fields--environmental management--fighter aircraft--life support systems (spacecraft)--mass transfer--space applications--structural design--wastes--alternating current--combustion method--individual components--mineral nutrients--optimal parameter--physicochemical methods--technical implementation--waste processing--waste incineration
Аннотация: Closed life support systems for space applications need a technology of processing organic waste produced in the system that would enable incorporating the recycled waste into the mass transfer of the system. Researchers of the Institute of Biophysics SB RAS have developed a method of waste processing that meets these requirements: a physicochemical method of organic waste oxidation in the hydrogen peroxide aqueous solution under application of an alternating current electric field - wet combustion. The mineralized solution produced by this method can be used as a mineral nutrient supplement for higher plants in the life support system. The present study describes technical implementation of the wet combustion method and reports results of developing this method in the last few years. The study addresses problems associated with the design and positions of individual components and different configurations of the wet combustion reactor, showing the way to automate operation of the reactor and reporting optimal parameters of the current applied to the electrodes, which reduce time and power consumption by waste processing. © 2018 Institute of Physics Publishing. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kotlobay A. A., Sarkisyan K. S., Mokrushina Y. A., Marcet-Houben M., Serebrovskaya E. O., Markina N. M., Somermeyer L. G., Gorokhovatsky A. Y., Vvedensky A., Purtov K. V., Petushkov V. N., Rodionova N. S., Chepurnyh T. V., Fakhranurova L. I., Guglya E. B., Ziganshin R., Tsarkova A. S., Kaskova Z. M., Shender V., Abakumov M., Abakumova T. O., Povolotskaya I. S., Eroshkin F. M., Zaraisky A. G., Mishin A. S., Dolgov S. V., Mitiouchkina T. Y., Kopantzev E. P., Waldenmaier H. E., Oliveira A. G., Oba Y., Barsova E., Bogdanova E. A., Gabaldon T., Stevani C. V., Lukyanov S., Smirnov I. V., Gitelson J. I., Kondrashov F. A., Yampolsky I. V.
Заглавие : Genetically encodable bioluminescent system from fungi
Место публикации : Proc. Natl. Acad. Sci. U. S. A.: National Academy of Sciences, 2018. - Vol. 115, Is. 50. - С. 12728-12732. - ISSN 00278424 (ISSN) , DOI 10.1073/pnas.1803615115
Ключевые слова (''Своб.индексиров.''): bioluminescence--fungal luciferase--fungal luciferin biosynthesis
Аннотация: Bioluminescence is found across the entire tree of life, conferring a spectacular set of visually oriented functions from attracting mates to scaring off predators. Half a dozen different luciferins, molecules that emit light when enzymatically oxidized, are known. However, just one biochemical pathway for luciferin biosynthesis has been described in full, which is found only in bacteria. Here, we report identification of the fungal luciferase and three other key enzymes that together form the biosynthetic cycle of the fungal luciferin from caffeic acid, a simple and widespread metabolite. Introduction of the identified genes into the genome of the yeast Pichia pastoris along with caffeic acid biosynthesis genes resulted in a strain that is autoluminescent in standard media. We analyzed evolution of the enzymes of the luciferin biosynthesis cycle and found that fungal bioluminescence emerged through a series of events that included two independent gene duplications. The retention of the duplicated enzymes of the luciferin pathway in nonluminescent fungi shows that the gene duplication was followed by functional sequence divergence of enzymes of at least one gene in the biosynthetic pathway and suggests that the evolution of fungal bioluminescence proceeded through several closely related stepping stone nonluminescent biochemical reactions with adaptive roles. The availability of a complete eukaryotic luciferin biosynthesis pathway provides several applications in biomedicine and bioengineering. © 2018 National Academy of Sciences. All Rights Reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shershneva A. M., Murueva A. V., Zhila N. O., Volova T. G.
Заглавие : Antifungal activity of P3HB microparticles containing tebuconazole
Место публикации : J. Environ. Sci. Health Part B Pestic. Food Contamin. Agric. Wastes: Taylor and Francis Inc., 2019. - Article in press. - ISSN 03601234 (ISSN) , DOI 10.1080/03601234.2018.1550299
Ключевые слова (''Своб.индексиров.''): antifungal activity--encapsulation--microparticles--poly-3-hydroxybutyrate--tebuconazole--agricultural chemicals--emulsification--encapsulation--fungicides--anti-fungal activity--average diameter--encapsulation efficiency--fusarium moniliforme--micro-particles--phytopathogenic fungi--poly-3-hydroxybutyrate--tebuconazole--fungi
Аннотация: In this study, tebuconazole (TEB)-loaded poly-3-hydroxybutyrate (P3HB)-based microparticles were developed and comprehensively characterized. TEB-loaded microparticles with the initial loading amounts of the fungicide of 10, 25, and 50% of the polymer mass (TEB 10, TEB 25, and TEB 50%) were prepared using emulsion technique. Encapsulation efficiency of TEB varied from 59 to 86%. As the loading amount was increased, the average diameter of microparticles increased too, from 41.3 to 71.7 µm, while zeta potential was not influenced by TEB loading, varying between –32.6 and –35.7 mV. TEB was gradually released from the microparticles to the model medium, and after 60 d, from 25 to 43% of TEB was released depending on the content of the encapsulated fungicide. The data obtained from in vitro TEB release were fitted to different mathematical models. It was shown that the release profiles of TEB could be best explained by the Zero-order, Higuchi, and Hixson–Crowell models. The antifungal activity of the P3HB/TEB microparticles against phytopathogenic fungi Fusarium moniliforme and Fusarium solani was demonstrated by in vitro tests conducted in Petri dishes. Thus, hydrophobic agrochemicals (TEB) can be effectively encapsulated into P3HB microparticles to construct slow-release formulations. © 2019, © 2019 Informa UK Limited, trading as Taylor & Francis Group.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Karpowicz M., Feniova I., Gladyshev M. I., Ejsmont-Karabin J., Gorniak A., Zielinski P., Dawidowicz P., Kolmakova A. A., Dzialowski A. R.
Заглавие : The stoichiometric ratios (C:N:P) in a pelagic food web under experimental conditions
Место публикации : Limnologica: Elsevier GmbH, 2019. - Vol. 77. - Ст.125690. - ISSN 00759511 (ISSN) , DOI 10.1016/j.limno.2019.125690
Аннотация: Interactions between phytoplankton and zooplankton affect the overall functioning of lakes. Herbivores are habitually confronted with food of inferior quality, usually a result of low nutrient concentrations in plant material. Large-bodied cladocerans are better competitors for food than small-bodied species but they are more vulnerable to low food quality. Understanding the effects of food quality on zooplankton structure and competition between small - large bodied herbivorous is of considerable interest. We want to find out how differences in C:N:P ratios between phytoplankton and zooplankton communities affect their abundances in a freshwater food web. We want also to assess the role of phytoplankton and zooplankton as sinks of the phosphorus and nitrogen. Therefore, we conducted a 31-day mesocosms experiment with water from a mesotrophic and a eutrophic lake (with natural plankton communities). To simulate changes in the plankton communities large-bodied Daphnia magna and Daphnia pulicaria were added. Samples for zooplankton, phytoplankton and water chemistry were taken every 10 days. Samples for elemental analysis (C:N:P) of seston and zooplankton were collected on the first, and on the final day of the experiment. Our mesocosms experiment showed mismatch in C:P between seston (high) and zooplankton (low), which suggests that most of the phosphorus is incorporated in zooplankton biomass. This evidenced that zooplankton is an effective sink of phosphorus, while nitrogen is accumulated mainly by primary producers. Our results also indicated more stability in stoichiometry with increasing trophic levels of organisms. However, there were significant changes in the zooplankton structure. The increasing dominance of large Daphnia resulted in reduction of C:P ratio in zooplankton. Low food quality (C:P) did not limit the growth of large Daphnia in the experimental conditions, which competed effectively with small planktonic cladocerans and with Rotifera. Over time, inedible algae began to dominate resulting in increase of relative biomass of periphyton grazers, which suggests that plankton community is transformed into littoral system in mesocosms for about 30 days. © 2019
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A., Melgunov M.
Заглавие : Radioactive particles in the Yenisei River floodplain (Russia): Characterization, leaching and potential effects in the environment
Место публикации : J. Environ. Radioact.: Elsevier Ltd, 2019. - Vol. 208-209. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2019.105991
Аннотация: The operation of the Mining-and-Chemical Combine (MCC), the largest producer of weapons-grade plutonium in Russia, has resulted in radioactive contamination of the Yenisei River floodplain. Investigations carried out in Novosibirsk and Krasnoyarsk institutes have shown that the floodplain of the Yenisei downstream of the MCC is contaminated by radioactive particles (RP) of various types and activities. Analytical characterization of the RP showed that most of them were fuel particles, which were carried into the Yenisei after incidents at the MCC reactors. The plutonium and caesium isotope ratios (238Pu/239,240Pu; 137Cs/134Cs) vary substantially between the particles, indicating different source terms and time intervals when the RP were formed. In addition to fuel RP, there were particles that contained activation radionuclides. The experiment on dissolution of RP using the model solution (the simulated stomach fluid) showed different cumulative extractions of radionuclides from the particles: 60Co and 137Cs extractions were the lowest, the extracted fractions of europium and americium isotopes were the largest, and plutonium occupied an intermediate position. High concentrations of radionuclides in RP are sources of exposure of organisms in aquatic and terrestrial ecosystems to low radiation doses. The plant bioassays of the effects of ?-radiation from RP showed the effect of low doses of ?-radiation on growth parameters of aquatic plant Elodea canadensis growing in the Yenisei River floodplain. The presence of RP from different sources in the Yenisei River floodplain makes this region a unique site for studying environmental effects of the particles. © 2019 Elsevier Ltd
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky A., Dementyev D., Trofimova E., Iniatkina E., Kladko Y., Petrichenkov M.
Заглавие : Chromosomal aberrations and micronuclei induced in onion (Allium cepa) by gamma-radiation
Место публикации : J. Environ. Radioact.: Elsevier Ltd, 2019. - Vol. 207. - С. 1-6. - ISSN 0265931X (ISSN) , DOI 10.1016/j.jenvrad.2019.05.014
Аннотация: The Allium-test is commonly used to assess genotoxicity of chemical and physical factors. In the present study, the roots of germinating onion (Allium cepa) were exposed to 0.02–13 Gy of ?-radiation. The dose dependencies of the frequency of chromosomal aberrations and micronuclei were nonlinear. An increase in the frequency of chromosomal aberrations in germinating seed root cells was first found under exposure to low doses of ?-radiation (0.05 and 0.1 Gy). Micronuclei inductions at low doses of radiation were not significantly different from the control. Our study suggests that germinating onion seed roots are a sensitive bioassay material for assessing the genotoxic effects of low-dose ?-radiation. © 2019 Elsevier Ltd
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N. N., Zuev I. V., Kalachova G. S., Ageev A. V., Gladyshev M. I.
Заглавие : Content of highly unsaturated fatty acids in fish from rivers of contrasting temperature
Колич.характеристики :10 с
Коллективы : Council on Grants from the President of the Russian Federation for Support of Leading Scientific Schools [NSh-9249.2016.5]; Russian Federal Tasks of Fundamental Research [51.1.1]; Federal Tasks of Ministry of Education and Science of the Russian Federation for Siberian Federal University [6.1504.2017/4.6]
Место публикации : River Res. Appl.: WILEY, 2018. - Vol. 34, Is. 6. - С. 565-574. - ISSN 1535-1459, DOI 10.1002/rra.3286. - ISSN 1535-1467(eISSN)
Примечания : Cited References:73. - Council on Grants from the President of the Russian Federation for Support of Leading Scientific Schools, Grant/Award Number: NSh-9249.2016.5; Russian Federal Tasks of Fundamental Research, Grant/Award Number: 51.1.1; Federal Tasks of Ministry of Education and Science of the Russian Federation for Siberian Federal University, Grant/Award Number: 6.1504.2017/4.6
Предметные рубрики: TROUT ONCORHYNCHUS-MYKISS
SALMON SALMO-SALAR
FRESH-WATER
LONG-CHAIN
Ключевые слова (''Своб.индексиров.''): benthivorous fish--climate warming--polyunsaturated fatty acids--river--water temperature
Аннотация: Contents of highly unsaturated long-chain fatty acids of omega-3 family (HUFA); eicosapentaenoic acid (EPA; 20:5n-3); and docosahexaenoic acid (DHA; 22:6n-3) are the principal indicators of the nutritive quality of fish for humans. Effects of environmental factors, first of all water temperature, on EPA and DHA contents in fish tissue are not currently completely understood. To reveal the putative effect of water temperature, fatty acid composition and contents were studied for 6 fish species, inhabiting the cold waters of the Yenisei River (Siberia, Russia) downstream of a dam (hypolimnetic release from reservoir) and its adjacent warm water tributaries (the Mana River and the Kacha River). It was hypothesized that (a) fish species from the cold river would have higher HUFA contents than fish from the warm rivers and (b) temperature would be negatively correlated with HUFA content in fish species. Using gas chromatography-mass spectrometry, distinct species-specific fatty acid profiles were observed, whereas contents of the essential fatty acids, EPA and DHA, in fish species from the cold Yenisei River were in general similar to that from warm tributaries. Thus, in contrast to the first hypothesis, phylogenetic factors overweighed the effect of water temperature and food (benthic invertebrate) composition, on fatty acid composition and HUFA content in fish. For the second hypothesis, for the 2 species inhabiting both cold and warm rivers, only one had higher EPA and DHA content at lower temperatures. Consequently, the response of EPA and DHA content in fish tissue to temperature variations may be species-specific.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev, Michail I., Sushchik, Nadezhda N., Tolomeev, Alexander P., Dgebuadze, Yury Yu
Заглавие : Meta-analysis of factors associated with omega-3 fatty acid contents of wild fish
Колич.характеристики :23 с
Коллективы : Russian Science Foundation [16-14-10001]
Место публикации : Rev. Fish. Biol. Fish.: SPRINGER, 2018. - Vol. 28, Is. 2. - С. 277-299. - ISSN 0960-3166, DOI 10.1007/s11160-017-9511-0. - ISSN 1573-5184(eISSN)
Примечания : Cited References:138. - The work was supported by a Russian Science Foundation Grant (No. 16-14-10001).
Предметные рубрики: FATTY-ACID-COMPOSITION
DIETARY DOCOSAHEXAENOIC ACID
LONG-CHAIN
Ключевые слова (''Своб.индексиров.''): docosahexaenoic acid--ecomorphological factors--eicosapentaenoic acid--nutritive value--phylogenetic factors
Аннотация: Fish are recognized as the main source of physiologically important omega-3 long-chain polyunsaturated fatty acids, namely, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), for human nutrition. However, muscle tissue contents of these fatty acids in diverse fish species, i.e., their nutritive value for humans, varied within two orders of magnitude. We reviewed contents of EPA and DHA, measured by similar methods using an internal standard during chromatography as mg per g of wet mass in 172 fish species belonging to 16 orders, to evaluate probable variations in phylogenetic and ecological drivers. EPA + DHA content varied from 25.6 mg g(-1) of wet mass (Sardinops sagax) to 0.12 mg g(-1) (Gymnura spp.). Multidimensional redundancy analysis revealed that among phylogenetic, ecomorphological and abiotic environmental factors, the highest proportion of variation contribution belonged to the shared contribution of sets of phylogenetic and ecomorphological factors. Specifically, the highest values of EPA + DHA content were characteristic of fish belonging to the orders Clupeiformes or Salmoniformes, were pelagic fast swimmers, ate zooplankton and inhabited marine waters or migrated from fresh to marine waters (anadromous migrations). High EPA and DHA content in muscle tissues of the above species appeared to be a metabolic adaptation for fast continuous swimming. In contrast to common beliefs, our meta-analysis did not support the significant influence of higher trophic levels (piscivory) and cold environments (homeoviscous adaptation) on EPA and DHA content in fish. However, many causes of high and low levels of physiologically important fatty acids in certain fish species remained unexplained and require evaluation in future studies.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Mogilnaya O. A., Ronzhin N. O., Bondar V. S.
Заглавие : Estimating levels of light emission and extracellular peroxidase activity of mycelium of luminous fungus Neonothopanus nambi treated with ?-glucosidase
Место публикации : Curr. Res. Environ. Appl. Mycol. J. Fungal: Beijing Academy of Agriculture and Forestry Sciences, Institute of Plant and Environment Protection, 2018. - Vol. 8, Is. 1. - С. 75-85. - ISSN 22292225 (ISSN) , DOI 10.5943/cream/8/1/6
Ключевые слова (''Своб.индексиров.''): basidiomycetes--cell wall--luminescence--polysaccharide sheath
Аннотация: The present study estimates the level of extracellular peroxidase activity and light emission intensity of mycelium of luminescent basidiomycete Neonothopanus nambi treated with ?-glucosidase. A hypothesis has been proposed that treatment with ?-glucosidase may trigger biochemical mechanisms of activation of ROS (primarily hydrogen peroxide) generation in N. nambi mycelium. The results obtained indicate that the enzyme causes partial disintegration of the slimy sheath of fungal hyphae and intracellular matrix, which leads to release of the extracellular peroxidases to the incubation medium. Mycelial cells treated with the enzyme reach the peak of their luminescence sooner. It has been assumed that partial loss of extracellular peroxidases, as important enzymes of antioxidant defense, may be compensated for by an increase in the level of light emission by the fungus. © 2018 Beijing Academy of Agriculture and Forestry Sciences.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Salmela, Anniina, Kokkonen, Eero, Kulmala, Ilpo, Veijalainen, Anna-Maria, van Houdt, Rob, Leys, Natalie, Berthier, Audrey, Viacheslav, Ilyin, Kharin, Sergey, Morozova, Julia, Tikhomirov, Alexander, Pasanen, Pertti
Заглавие : Production and characterization of bioaerosols for model validation in spacecraft environment
Колич.характеристики :12 с
Коллективы : European Union [263076]
Место публикации : J. Environ. Sci.: SCIENCE PRESS, 2018. - Vol. 69. - С. 227-238. - ISSN 1001-0742, DOI 10.1016/j.jes.2017.10.016. - ISSN 1878-7320(eISSN)
Примечания : Cited References:28. - The research leading to these results has received funding from the European Union Seventh Framework Programme (FP/2007-2013) under grant agreement number 263076 within the BIOSMHARS Project (BIO contamination Specific Modeling in Habitats Related to Space).
Предметные рубрики: INDOOR AIR
MICROBIAL-CONTAMINATION
BUILDING-MATERIALS
FUNGAL
Ключевые слова (''Своб.индексиров.''): bioaerosol--modeling--cfd--spacecraft--fungi--bacteria
Аннотация: This study aimed to evaluate the suitability of two bioaerosol generation systems (dry and wet generation) for the aerosolization of microorganisms isolated from the International Space Station, and to calibrate the produced bioaerosols to fulfill the requirements of computational fluid dynamics model (CFD) validation. Concentration, stability, size distribution, agglomeration of generated bioaerosol and deposition of bioaerosols were analyzed. In addition, the dispersion of non-viable particles in the air was studied. Experiments proved that wet generation from microbial suspensions could be used for the production of well-calibrated and stabile bioaerosols for model validation. For the simulation of the natural release of fungal spores, a dry generation method should be used. This study showed that the used CFD model simulated the spread of non-viable particles fairly well. The mathematical deposition model by Lai and Nazaroff could be used to estimate the deposition velocities of bioaerosols on surfaces, although it somewhat underestimated the measured deposition velocities. (c) 2017 The Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. Published by Elsevier B.V.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakova O. V., Gladyshev M. I., Fonvielle J. A., Ganzert L., Hornick T., Grossart H. -P.
Заглавие : Effects of zooplankton carcasses degradation on freshwater bacterial community composition and implications for carbon cycling
Место публикации : Environ. Microbiol.: Blackwell Publishing Ltd, 2018. - Article in press. - ISSN 14622912 (ISSN) , DOI 10.1111/1462-2920.14418
Аннотация: Non-predatory mortality of zooplankton provides an abundant, yet, little studied source of high quality labile organic matter (LOM) in aquatic ecosystems. Using laboratory microcosms, we followed the decomposition of organic carbon of fresh 13C-labelled Daphnia carcasses by natural bacterioplankton. The experimental setup comprised blank microcosms, that is, artificial lake water without any organic matter additions (B), and microcosms either amended with natural humic matter (H), fresh Daphnia carcasses (D) or both, that is, humic matter and Daphnia carcasses (HD). Most of the carcass carbon was consumed and respired by the bacterial community within 15 days of incubation. A shift in the bacterial community composition shaped by labile carcass carbon and by humic matter was observed. Nevertheless, we did not observe a quantitative change in humic matter degradation by heterotrophic bacteria in the presence of LOM derived from carcasses. However, carcasses were the main factor driving the bacterial community composition suggesting that the presence of large quantities of dead zooplankton might affect the carbon cycling in aquatic ecosystems. Our results imply that organic matter derived from zooplankton carcasses is efficiently remineralized by a highly specific bacterial community, but does not interfere with the bacterial turnover of more refractory humic matter. © 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Oskina N., Lopatina T., Anishchenko O., Zadereev E.
Заглавие : High Resistance of Resting Eggs of Cladoceran Moina macrocopa to the Effect of Heavy Metals
Место публикации : Bull. Environ. Contam. Toxicol.: Springer New York LLC, 2018. - Article in press. - ISSN 00074861 (ISSN) , DOI 10.1007/s00128-018-2473-7
Ключевые слова (''Своб.индексиров.''): hatching success--heavy metals--life table parameters--resistance--resting eggs--copper--electric resistance--aquatic habitats--critical concentration--environmental pollutions--hatching success--high resistance--lethal concentration--life table--resting eggs--heavy metals
Аннотация: The research aimed to determine critical concentrations of heavy metals at which survival of resting eggs of the cladoceran Moina macrocopa is negatively affected. Resting eggs’ viability was not affected over a 30-days exposure towards copper, cadmium, zinc or nickel at concentrations up to 60–70 g/L. When resting eggs were exposed to sediment contaminated with heavy metals for 8 months, the hatching success was affected at 30 g copper/kg. Thus, resting eggs of Cladocera can tolerate heavy metals at concentrations that far exceed lethal concentrations of heavy metals to active life stage and exceed low or moderate levels of environmental pollution. Follow up investigation of life table parameters of hatchlings from resting eggs exposed to heavy metals demonstrated that neither lifespan nor fecundity of hatchlings differ from control animals. These results demonstrate that zooplankton may rapidly recover from resting egg bank once aquatic habitat becomes unpolluted. © 2018, Springer Science+Business Media, LLC, part of Springer Nature.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Denisov I., Lukyanenko K., Yakimov A., Kukhtevich I., Esimbekova E., Belobrov P.
Заглавие : Disposable luciferase-based microfluidic chip for rapid assay of water pollution
Место публикации : Lumin. - 2018. - Vol. 33, Is. 6. - С. 1054-1061. - ISSN 15227235 (ISSN) , DOI 10.1002/bio.3508
Ключевые слова (''Своб.индексиров.''): bioassay--lab-on-a-chip--luciferase--microfluidics--solvent bonding
Аннотация: In the present study, we demonstrate the use of a disposable luciferase-based microfluidic bioassay chip for environmental monitoring and methods for fabrication. The designed microfluidic system includes a chamber with immobilized enzymes of bioluminescent bacteria Photobacterium leiognathi and Vibrio fischeri and their substrates, which dissolve after the introduction of the water sample and thus activate bioluminescent reactions. Limits of detection for copper (II) sulfate, 1,3-dihydroxybenzene and 1,4-benzoquinone for the proposed microfluidic biosensor measured 3 ?M, 15 mM, and 2 ?M respectively, and these values are higher or close to the level of conventional environmental biosensors based on lyophilized bacteria. Approaches for entrapment of enzymes on poly(methyl methacrylate) (PMMA) plates using a gelatin scaffold and solvent bonding of PMMA chip plates under room temperature were suggested. The proposed microfluidic system may be used with some available luminometers and future portable luminescence readers. © 2018 John Wiley & Sons, Ltd.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dementyev D., Bolsunovsky A.
Заглавие : A long-term study of radionuclide concentrations in mushrooms in the 30-km zone around the Mining-and-Chemical Combine (Russia)
Место публикации : Isot. Environ. Health Stud.: Taylor and Francis Ltd., 2020. - Article in press. - ISSN 10256016 (ISSN), DOI 10.1080/10256016.2020.1718124
Аннотация: 137Cs concentrations were measured in mushrooms in an area affected by radioactive discharges of the Mining-and-Chemical Combine (MCC) (Rosatom, Russia) in 2002–2017. The sources of radionuclides in the study sites were global fallouts and waterborne and airborne radioactive discharges of the MCC. The mushroom species Suillus granulatus and S. luteus showed the highest 137Cs concentrations (140–7100 Bq kg?1) for this area. Over the entire monitoring period, no significant change in 137Cs concentration was observed in the Suillus spp. samples collected from the sites with the aerial deposition of radionuclides. In the floodplain site with the radionuclide deposition from water and air, a significant decrease in the average 137Cs concentration was observed in the period between 2004 and 2017: a three-fold decrease in Suillus spp. and a nine-fold decrease in Lactarius deliciosus. The effective half-lives of 137Cs in fruiting bodies of the mushrooms L. deliciosus and Suillus spp. in this site were 3.6 ± 0.6 and 9.2 ± 2.7 years, respectively. © 2020, © 2020 Informa UK Limited, trading as Taylor & Francis Group.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Dementyev, Dmitry, Bolsunovsky, Alexander
Заглавие : A long-term study of radionuclide concentrations in mushrooms in the 30-km zone around the Mining-and-Chemical Combine (Russia)
Колич.характеристики :10 с
Место публикации : Isot. Environ. Health Stud.: TAYLOR & FRANCIS LTD. - ISSN 1025-6016, DOI 10.1080/10256016.2020.1718124. - ISSN 1477-2639(eISSN)
Примечания : Cited References:25
Предметные рубрики: CS-137
FOREST
RADIOCESIUM
FUNGI
ACCUMULATION
ACCIDENT
Аннотация: Cs-137 concentrations were measured in mushrooms in an area affected by radioactive discharges of the Mining-and-Chemical Combine (MCC) (Rosatom, Russia) in 2002-2017. The sources of radionuclides in the study sites were global fallouts and waterborne and airborne radioactive discharges of the MCC. The mushroom species Suillus granulatus and S. luteus showed the highest Cs-137 concentrations (140-7100 Bq kg(-1)) for this area. Over the entire monitoring period, no significant change in Cs-137 concentration was observed in the Suillus spp. samples collected from the sites with the aerial deposition of radionuclides. In the floodplain site with the radionuclide deposition from water and air, a significant decrease in the average Cs-137 concentration was observed in the period between 2004 and 2017: a three-fold decrease in Suillus spp. and a nine-fold decrease in Lactarius deliciosus. The effective half-lives of Cs-137 in fruiting bodies of the mushrooms L. deliciosus and Suillus spp. in this site were 3.6 +/- 0.6 and 9.2 +/- 2.7 years, respectively.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bolsunovsky, Alexander, Dementyev, Dmitry, Trofimova, Elena
Заглавие : Biomonitoring of radioactive contamination of the Yenisei River using aquatic plants
Колич.характеристики :12 с
Коллективы : Krasnoyarsk Regional Fund of Science - Russian Foundation for Basic Research [18-44-240001]
Место публикации : J. Environ. Radioact.: ELSEVIER SCI LTD, 2020. - Vol. 211. - Ст.106100. - ISSN 0265-931X, DOI 10.1016/j.jenvrad.2019.106100. - ISSN 1879-1700(eISSN)
Примечания : Cited References:39. - The authors are grateful to researchers T. Zotina, M. Medvedeva, A. Zueva, E. Iniatkina, and Yu. Kladko of the Institute of Biophysics SB RAS (Krasnoyarsk, Russia) for their assistance in collecting plant samples and conducting cytogenetic analysis. The study was partly supported by grant of the Krasnoyarsk Regional Fund of Science - Russian Foundation for Basic Research No. 18-44-240001.
Предметные рубрики: GAMMA-EMITTING RADIONUCLIDES
ELODEA-CANADENSIS
NONHUMAN BIOTA
Аннотация: The Yenisei River is contaminated by artificial radionuclides released by one of the Russian facilities producing weapons-grade plutonium (the Mining-and-Chemical Combine, MCC), which has been in operation for 60 years. The paper presents results of long-term monitoring of radionuclide concentrations in six aquatic plant species collected from the 1400 km Yenisei River stretch downstream of the city of Krasnoyarsk. Before the last MCC reactor was shut down (in 2010), up to 30 artificial radionuclides were detected in the plant biomass, and 2 and 5 years after the reactor shutdown, 11 and 3-5 radionuclides, respectively, were detected. The highest concentrations of radionuclides were recorded in Fontinalis antipyretica. The aquatic plant Elodea canadensis, which commonly occurs in the Yenisei River, was used in the cytogenetic study. High frequencies of chromosomal aberrations (up to 33%) were revealed in cells of Elodea canadensis roots from the Yenisei region affected by the MCC radioactive discharge, at dose rates of 45-72 mu Gy/d, while in the cells of the plant roots from the reference areas, the frequency of chromosomal aberrations was 5-7%, at dose rates below 2 mu Gy/d. The higher frequencies of chromosomal aberrations in Elodea canadensis collected from the parts of the River with increased concentrations of artificial radionuclides are associated with the radiation factor. The aquatic plant Elodea canadensis can be recommended as a bio-indicator for radioactively contaminated aquatic ecosystems.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feio, Maria Joao, Filipe, Ana Filipa, Garcia-Raventos, Aina, Ardura, Alba, Calapez, Ana Raquel, Pujante, Ana Maria, Mortagua, Andreia, Murria, Cesc, Diaz-de-Quijano, Daniel, Martins, Filipa M. S., Duarte, Sofia, Bariain, Marta Sainz, Cordeiro, Rita, Rivera, Sinziana F., Vaisanen, Leif O. S., Fonseca, Amelia, Goncalves, Vitor, Garcia-Vazquez, Eva, Rodriguez, David Vieites, Ivanova, Elena A., Costa, Filipe O., Barquin, Jose, Rojo, Veronica, Vierna, Joaquin, Fais, Maria, Suarez, Marcos, Nieminen, Marko, Hammers-Wirtz, Monica, Kolmakova, Olesia, V, Trusova, Maria Y., Beja, Pedro, Gonzalez, Raquel, Planes, Serge, Almeida, Salome F. P.
Заглавие : Advances in the use of molecular tools in ecological and biodiversity assessment of aquatic ecosystems
Колич.характеристики :22 с
Коллективы : MARE strategic program [UID/MAR/04292/2013]; GeoBioTec strategic program [UID/GEO/04035/2019]; Fundo Regional da Ciencia e Tecnologia (FRCT) [M3.1.a/F/017/2011]; FRESHING Project "Next-generation biomonitoring: freshwater bioassessment and species conservation improved with metagenomics" - Portuguese Foundation for Science and Technology (FCT); COMPETE [PTDC/AAG-MAA/2261/2014 -POCI-01-0145-FEDER-356 016824]; FCTPortuguese Foundation for Science and Technology [SFRH/BD/104703/2014, SFRH/BD/113547/2015, SFRH/BPD/109842/2015]; FCT-PhD programme FLUVIO [PD\BD\52510\2014]; Eurostars project [BIOWAT-KIT_E!11892]; Fundacio Aigues de Barcelona
Место публикации : Limnetica: ASOC ESPAN LIMNOL-MISLATA, 2020. - Vol. 39: 19th Congress of the Iberian-Association-of-Limnology (AIL) (JUN 24-29, 2018, Coimbra, PORTUGAL), Is. 1. - С. 419-440. - ISSN 0213-8409, DOI 10.23818/limn.39.27. - ISSN 1989-1806(eISSN)
Примечания : Cited References:92. - We are grateful to all participants of the special session "The use of molecular tools in ecological and biodiversity assessment of aquatic ecosystems" for the productive discussions during the AIL 2018 meeting (XIX Iberian Association of Limnology meeting in Coimbra (Portugal, June 2018). M.J. Feio is supported by MARE strategic program (UID/MAR/04292/2013); SFP Almeida is supported by GeoBioTec strategic program UID/GEO/04035/2019. R. Cordeiro was supported by a Ph.D. Grant (M3.1.a/F/017/2011) from Fundo Regional da Ciencia e Tecnologia (FRCT); A.F. Filipe and A. Garcia-Raventos were supported by FRESHING Project "Next-generation biomonitoring: freshwater bioassessment and species conservation improved with metagenomics" funded by the Portuguese Foundation for Science and Technology (FCT) and COMPETE (PTDC/AAG-MAA/2261/2014 -POCI-01-0145-FEDER-356 016824); F.M.S. Martins was supported by a FCT PhD grant (SFRH/BD/104703/2014); A.R. Calapez was supported by a grant from the FCT-PhD programme FLUVIO (PD\BD\52510\2014); A.M. Pujante acknowledges the BIOWAT-KIT_E!11892 Eurostars project; Maria Fais and Sofia Duarte were supported, respectively, by a PhD (SFRH/BD/113547/2015) and a post-doc fellowship (SFRH/BPD/109842/2015), from FCT; and C. Murria acknowledges the Fundacio Aigues de Barcelona for funding his research.
Предметные рубрики: BARCODE REFERENCE LIBRARY
METABARCODING APPROACH
RAPID ASSESSMENT
Аннотация: Conservation and sustainable management of aquatic ecosystems is a priority in environmental programs worldwide. However, these aims are highly dependent on the efficiency, accuracy and cost of existent methods for the detection of keystone species and monitoring of biological communities. Rapid advances in eDNA, barcoding and metabarcoding promoted by high-throughput sequencing technologies are generating millions of sequences in a fast way, with a promising cost reduction, and overcoming some difficulties of the traditional taxonomic approaches. This paper provides an updated broad perspective of the current developments in this dynamic field presented in the special session (SS) "The use of molecular tools in ecological and biodiversity assessment of aquatic ecosystems" of the XIX Congress of the Iberian Association of Limnology (AIL2018), held in Coimbra, Portugal. Developments presented are mainly focused on the Iberian Peninsula (Portugal and Spain, including Atlantic Macaronesian islands) but include studies in France, Germany, Finland, Russia (Siberia) and South America. The networks within which these researchers are involved are yet even broader, profiting from existing molecular facilities, and traditional taxonomic expertise, which can be viewed as a characteristic of this new research area. It was evident in the SS that the use of molecular tools is widespread, being used to study a diversity of aquatic systems, from rivers' headwaters to estuaries and coastal lagoons, and volcanic, mountain and frozen lakes to hot springs. The organisms targeted are likewise varied and include fish, macroinvertebrates, meiofauna, microalgae such as diatoms and dinoflagellates, other protists, fungi, and bacteria (cyanobacteria and other). Some studies address the whole biodiversity (i.e., all species present independently of the taxonomic group) from environmental samples of water, biofilms and preservative solution from field samples (e.g., ethanol from macroinvertebrate samples). Great advances were acknowledged in the special session, namely in the use of metabarcoding for detecting hidden biodiversity, juvenile stages, low-abundance species, non-indigenous species and toxicity potential, and ultimately for ecological monitoring of diatoms and invertebrates. Yet, several drawbacks were highlighted and need further work, which include: taxonomic gaps in the reference databases (including gaps at species level and on intraspecific variability) or absence of public databases (e.g. for meiofauna), still high sequencing costs, the need of a substantial bioinformatics effort, difficulties in establishing the amount of environmental sample necessary for a good DNA extraction and the need for testing different genetic markers to obtain accurate results.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Polyakova G., Pashenova N., Senashova V., Podolyak N., Kudryasheva N.
Заглавие : Pine stands as bioindicators: Justification for air toxicity monitoring in an industrial metropolis
Место публикации : Environ. - MDPI: MDPI AG, 2020. - Vol. 7, Is. 4. - Ст.28. - ISSN 20763298 (ISSN), DOI 10.3390/environments7040028
Аннотация: Five permanent sample plots (SPs; 200-250 trees per plot) were established in middleaged high-grade suburban pine stands near the industrial city of Krasnoyarsk, Siberia, Russia. Needle damage, inventory parameters of the stands, and the defense response of the stem phloem were evaluated annually for the years 2002-2019 and attributed to acute or chronic toxic exposures (creeping fire or industrial pollutants, respectively). The results form a basis for using trees as bioindicators. A newly elaborated stem lesion test was formed from a hypothesis on the upward sugar transport for the regeneration of an injured crown, based on Eschrich‘s model of bidirectional sugar transport in the phloem. The formation of a phloem lesion was induced by inoculation of the stem with a mycelial extract of the ophiostomatoid fungus Ceratocystis laricicola. The lesion length and its shift relative to the inoculation hole were measured. An increase in the length of needles at early stages of stand weakening by pollutants was found to correspond to the hormesis model (Selye‘s adaptation syndrome). A possibility of assessing the chronology of pollutant toxicity and the duration of the recovery period after creeping fire was shown. © 2020 by the authors.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Polyakova, Galina, Pashenova, Natalia, Senashova, Vera, Podolyak, Natalia, Kudryasheva, Nadezhda
Заглавие : Pine Stands as Bioindicators: Justification for Air Toxicity Monitoring in an Industrial Metropolis
Колич.характеристики :13 с
Коллективы : Russian Academy of SciencesRussian Academy of Sciences [0356-2018-0742, 0356-2017-0017]; RFBRRussian Foundation for Basic Research (RFBR) [15-04-06575]; RFBR-Krasnoyarsk Regional Foundation [18-44-240004, 18-44-242002]
Место публикации : Environments: MDPI, 2020. - Vol. 7, Is. 4. - Ст.28. - ISSN 2076-3298(eISSN), DOI 10.3390/environments7040028
Примечания : Cited References:35. - This work was supported by the state budget allocated to the fundamental research at the Russian Academy of Sciences, projects 0356-2018-0742 and 0356-2017-0017, and by RFBR grant 15-04-06575, RFBR-Krasnoyarsk Regional Foundation N 18-44-240004, grant 18-44-242002.
Предметные рубрики: HORMESIS
Аннотация: Five permanent sample plots (SPs; 200-250 trees per plot) were established in middle-aged high-grade suburban pine stands near the industrial city of Krasnoyarsk, Siberia, Russia. Needle damage, inventory parameters of the stands, and the defense response of the stem phloem were evaluated annually for the years 2002-2019 and attributed to acute or chronic toxic exposures (creeping fire or industrial pollutants, respectively). The results form a basis for using trees as bioindicators. A newly elaborated stem lesion test was formed from a hypothesis on the upward sugar transport for the regeneration of an injured crown, based on Eschrich's model of bidirectional sugar transport in the phloem. The formation of a phloem lesion was induced by inoculation of the stem with a mycelial extract of the ophiostomatoid fungus Ceratocystis laricicola. The lesion length and its shift relative to the inoculation hole were measured. An increase in the length of needles at early stages of stand weakening by pollutants was found to correspond to the hormesis model (Selye's adaptation syndrome). A possibility of assessing the chronology of pollutant toxicity and the duration of the recovery period after creeping fire was shown.
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