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^a343.17.05^2VINITI
П 31

Петушков, В. Н.
Биолюминесцентный анализ ферментных систем [Текст] : научное издание / В. Н. Петушков // Молекул. механизмы клеточ. гомеостаза. Матер. шк., Красноярск, 13-22 марта, 1984. - Новосибирск, 1987. - С. 204-216

ГРНТИ

34.17.05

РУБ 343.17.05
Рубрики:
ФЕРМЕНТ
   АКТИВНОСТЬ
   ИЗМЕРЕНИЕ
   БИОЛЮМИНЕСЦЕНЦИЯ
   ЛЮЦИФЕРАЗА
   ПРИМЕНЕНИЕ
   ENZYMES
   В О М Е СЕ СЕ
   С Е А А
: 660036, Красноярск, Академгородок, д. 50, стр. 50

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^a343.15.33^2VINITI
К 78

Кратасюк, В. А.
Использование светящихся бактерий в биолюминесцентном анализе [Текст] : научное издание / В. А. Кратасюк, И. И. Гительзон // Успехи микробиол. - 1987. - N 21. - С. 3-30 . - ISSN 0566-392X

ГРНТИ

34.15.33

РУБ 343.15.33
Рубрики:
БАКТЕРИИ
   СВЕТЯЩИЕСЯ КУЛЬТУРЫ
   СВОЙСТВА
   ИММОБИЛИЗОВАННЫЙ ФЕРМЕНТ
   ПОЛУЧЕНИЕ
   ПРИМЕНЕНИЕ
   БИОЛЮМИНЕСЦЕНТНЫЙ АНАЛИЗ
   ОБЩИЕ ПРИНЦИПЫ
   ОБЗОРЫ
   БИБЛ 161
   IMMOBILIZED ENZYMES
   В О М Е СЕ СЕ

Доп.точки доступа:
Гительзон, И.И.

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^a314.27.17.05.05^2VINITI
Б 81

Бондарь, В. С.
Получение высокоочищенной глутатионредуктазы из гидроидных полипов Obelia longissima и изучение некоторых ее физико-химических свойств [Текст] : научное издание / В. С. Бондарь, Е. С. Высоцкий, В. Н. Летунов ; Академия наук СССР. Сибирское отделение. Институт биофизики, АН СССР. СО. Ин-т биофиз. // Препр. - 1990. - N 123. - С. 1-32

ГРНТИ

31.27.17

РУБ 314.27.17.05.05
Рубрики:
ГЛУТАТИОНРЕДУКТАЗА
   ВЫСОКООЧИЩЕННАЯ
   ПОЛУЧЕНИЕ
   СВОЙСТВА
   ПОЛИПЫ ГИДРОИДНЫЕ
   OBELIA LONGISSIMA
   ENZYMES
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Высоцкий, Е.С.; Летунов, В.Н.; Академия наук СССР. Сибирское отделение. Институт биофизики

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Why does the bioluminescent fungus Armillaria mellea have luminous mycelium but nonluminous fruiting body? / K. V. Purtov [et al.] // Doklad. Biochem. Biophys. - 2017. - Vol. 474, Is. 1. - P217-219, DOI 10.1134/S1607672917030176 . - ISSN 1607-6729
Аннотация: By determining the components involved in the bioluminescence process in luminous and nonluminous organs of the honey fungus Armillaria mellea, we have established causes of partial luminescence of this fungus. The complete set of enzymes and substrates required for bioluminescence is formed only in the mycelium and only under the conditions of free oxygen access. Since the synthesis of luciferin precursor (hispidin) and 3-hydroxyhispidin hydroxylase in the fruiting bodies is blocked, the formation of luciferin—the key component of fungal bioluminescent system—was not observed. That is why the fruiting body of Armillaria mellea is nonluminous despite the presence of luciferase, the enzyme that catalyzes the oxidation of luciferin with a photon emission. © 2017, Pleiades Publishing, Ltd.

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Держатели документа:
Institute of Biophysics, Krasnoyarsk Research Center, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Purtov, K. V.; Petushkov, V. N.; Rodionova, N. S.; Gitelson, J. I.

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The use of glowing wood as a source of luminescent culture of fungus mycelium [Text] / A. P. Puzyr, S. E. Medvedeva, V. S. Bondar // Mycosphere. - 2016. - Vol. 7, Is. 1. - P1-17, DOI 10.5943/mycosphere/7/1/1. - Cited References:22. - The authors are grateful to Prof. A. Frank, Director of North Borneo Biostation, for the opportunity to carry out studies of glowing wood; to Nadezhda N. Kudashova, a senior researcher at the Institute of Biology and Biophysics at the Tomsk University, for identifying the species of nonluminous fungi. This study was supported by grant no. 11.G34.31.0058 (RF Government) and Projects no. 71 (SB RAS). . - ISSN 2077-7000

РУБ Mycology
Рубрики:
BIOLUMINESCENCE CHARACTERISTICS
NEONOTHOPANUS-NAMBI
LIGHT-EMISSION
Кл.слова (ненормированные):
Bioluminescence -- culture of luminous mycelia -- kinetics of luminescent -- reaction -- light emitting wood -- luminous fungus
Аннотация: In studies of fungal bioluminescence, not only fruiting bodies and spores of the fungus, but also samples of luminescent wood, leaf litter or soil may need to be used to derive pure mycelial culture. This study describes an approach to isolating the culture of luminescent fungal mycelium from samples of light-emitting wood found on Borneo Island in November-December 2013. A GelDoc XR Imaging System (Bio-Rad Laboratories, Inc., U.S.) was used for the first time to monitor luminescence and select luminous samples. This study shows that for successful isolation of the culture of luminescent mycelium out of the luminescent wood found in the forest, it is imperative to keep the samples moist (mycelium alive until there is water), while immediate and aseptic delivery of the samples to the laboratory is not a crucial condition (inner layers of wood is "sterile"). Investigation of the growth features of the isolated mycelium in various growing conditions revealed some peculiar properties of its luminescence in comparison with the known luminescent cultures of basidiomycetes. When grown on solid nutrient media, mycelium exhibits low growth rates, long-lasting luminescence (140 days or longer), and emergence and disappearance of local zones with high levels of light emission. Mycelium produced in submerged culture does not emit light, and this effect must be caused by the absence or a very low level of the luminescent reaction substrate in the biomass. The luminescence system isolated from mycelial biomass did not induce luminescent reaction in vitro upon the addition of NADPH (recording intensity is 60 100 URL/sec). We found that enzymes of the luminescence systems isolated from mycelium pellets retained their activity and catalyzed luminescent reaction when a hot extract of the luminous fungus Armillaria sp. (IBSO 2360) was added (near 1900 URL/sec). The same effect was obtained after addition of hot extracts from the fruiting bodies of nonluminous higher fungi Pholiota squarrosa, Cortinarius sp., Hypholoma capnoides and Chroogomphus rutilus (near 3500 URL/sec). The pure culture of luminescent mycelium has been registered in the Culture Collection of IBP SB RAS as IBSO 2371; now it can be used for various in vivo and in vitro studies, including identification of the fungus.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Puzyr, A. P.; Medvedeva, S. E.; Bondar, V. S.; RF Government [11.G34.31.0058]; SB RAS [71]

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The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha / T. G. Volova, G. S. Kalacheva // Mikrobiologiya. - 2005. - Vol. 74, Is. 1. - С. 63-69 . - ISSN 0026-3656
Кл.слова (ненормированные):
?-ketothiolase -- Controlled synthesis -- Poly(hydroxybutyrate-co-hydroxyvalerate) -- Ralstonia eutropha -- Bacteria (microorganisms) -- Cupriavidus necator -- acetoacetyl coenzyme a -- acetyl coenzyme A acyltransferase -- acyl coenzyme A -- acyltransferase -- butyric acid derivative -- carbon dioxide -- fructose -- hydrogen -- poly(3 hydroxybutyrate) co (3 hydroxyvalerate) -- poly(3-hydroxyalkanoic acid) synthase -- poly(3-hydroxybutyrate)-co-(3-hydroxyvalerate) -- polyester -- polyhydroxyalkanoate synthase -- valeric acid derivative -- article -- chemistry -- crystallization -- culture medium -- metabolism -- Wautersia eutropha -- Acetyl-CoA C-Acyltransferase -- Acyl Coenzyme A -- Acyltransferases -- Butyrates -- Carbon Dioxide -- Crystallization -- Culture Media -- Cupriavidus necator -- Fructose -- Hydrogen -- Polyesters -- Valerates
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass, the yield, synthesis rate, and composition of copolymers, the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis (?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase), the maximum tolerable concentration of valerate to the bacterium, and the conditions that govern the incorporation of hydroxyvalerate to copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of these copolymers depend on the molar fraction of 3HV.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.

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The synthesis of hydroxybutyrate and hydroxyvalerate copolymers by the bacterium Ralstonia eutropha / T. G. Volova, G. S. Kalacheva // Microbiology. - 2005. - Vol. 74, Is. 1. - P54-59, DOI 10.1007/s11021-005-0028-5 . - ISSN 0026-2617
Кл.слова (ненормированные):
?-ketothiolase -- Controlled synthesis -- Poly(hydroxybutyrate-co-hydroxyvalerate) -- Ralstonia eutropha -- Bacteria (microorganisms) -- Ralstonia -- Wautersia eutropha
Аннотация: The paper deals with the study of the synthesis of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) copolymers by the bacterium Ralstonia eutropha B-5786 grown under different carbon nutrition conditions (growth on carbon dioxide, fructose, and CO2-valerate and fructose-valerate mixtures). The parameters to be analyzed included the yield of biomass; the yield, synthesis rate, and composition of copolymers; the activity of the key enzymes of polyhydroxyalkanoate (PHA) synthesis ?-ketothiolase, acetoacetyl-CoA reductase, and PHA synthase); the maximum tolerable concentration of valerate to the bacterium; and the conditions that govern the incorporation of hydroxyvalerate into copolymers. This allowed the relationship between cultivation conditions and the proportion of monomers in the copolymers to be deduced. We were able to synthesize a range of 3HB/3HV copolymers and found that the thermal characteristics and the degree of crystallinity of such copolymers depend on the molar fraction of 3HV. В© 2005 Pleiades Publishing, Inc.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.

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The autotrophic synthesis of polyhydroxyalkanoate by alcaligenes eutrophus in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 745-752 . - ISSN 0026-3656
Кл.слова (ненормированные):
CO inhibition -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates (PHAs) -- Synthesis -- alkane -- carbon monoxide -- fatty acid -- hydroxyacid -- lipid -- nitrogen -- polyester -- article -- chemistry -- culture medium -- drug antagonism -- growth, development and aging -- mass spectrometry -- metabolism -- Wautersia eutropha -- Alkanes -- Carbon Monoxide -- Culture Media -- Cupriavidus necator -- Fatty Acids -- Hydroxy Acids -- Lipids -- Mass Spectrometry -- Nitrogen -- Polyesters
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Microbiology. - 2001. - Vol. 70, Is. 6. - P640-646 . - ISSN 0026-2617
Кл.слова (ненормированные):
CO inhibition -- Hydrogen-oxidizing bacteria -- Polyhydroxyalkanoates (PHAs) -- Synthesis
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen. В© 2001 MAIK "Nauka/Interperiodica".

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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10.

The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide [Text] / T. G. Volova, G. S. Kalacheva, O. V. Altukhova // Microbiology. - 2001. - Vol. 70, Is. 6. - P. 640-646, DOI 10.1023/A:1013175413013. - Cited References: 19 . - ISSN 0026-2617

РУБ Microbiology

Кл.слова (ненормированные):
hydrogen-oxidizing bacteria -- polyhydroxyalkanoates (PHAs) -- synthesis -- CO inhibition
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-beta-ketothiolase. acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % beta-hydroxybutyrate and trace amounts of beta-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.

WOS
Держатели документа:
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Kalacheva, G.S.; Altukhova, O.V.

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11.

Study of the biological properties of polyoxyalkanoates in the in vivo chronic experiment / E. I. Shishatskaya, T. G. Volova, T. G. Popova // Meditsinskaya Tekhnika. - 2002. - Is. 4. - С. 29-33 . - ISSN 0025-8075
Кл.слова (ненормированные):
biomaterial -- polymer -- animal -- article -- bioremediation -- comparative study -- female -- metabolism -- rat -- suture -- Wistar rat -- Animals -- Biocompatible Materials -- Biodegradation, Environmental -- Female -- Polymers -- Rats -- Rats, Wistar -- Sutures -- Biocompatibility -- Immunology -- Implants (surgical) -- Materials testing -- Organic polymers -- Polyoxyalkanoates -- Rat models -- Suture threads -- Biomaterials
Аннотация: Chronic experiments on mature Wistar rats studied the biological properties of suture threads from two types of polyoxyalkanoates (POA), such as polyoxybutyrate (POB) and the copolymer of POB and polyoxyvalerate (POV) (POB/POV) as compared to the conventional surgical material (silk and catgut). The physiological and biochemical parameters that reflect the status of the animals, including their behavior, height and development, the weight of the body and viscera, the morphology of peripheral blood, the biochemical parameters of blood, the activity of some enzymes, as well as histological findings of the viscera, and lymphoid tissue responses were assessed. POA was found to produce no negative effect on the physiological, biochemical, and functional characteristics of the animals irrespective of the duration of contact with the internal medium of the body and of the chemical composition of a material.

Scopus
Держатели документа:
Inst. Biofiziki SO RAN, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.; Popova, T.G.

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12.

Structural distinctions of fast and slow bacterial luciferases revealed by phylogenetic analysis [Text] / A. A. Deeva [et al.] // Bioinformatics. - 2016. - Vol. 32, Is. 20. - P3053-3057, DOI 10.1093/bioinformatics/btw386. - Cited References:31. - The reported study was partially funded by RFBR according to the research project No. 16-34-00746 mol_a; by the Ministry of Education and Science of the Russian Federation [project No 1762] and by the state budget allocated to the fundamental research at the Russian Academy of Sciences [project No 01201351504]. . - ISSN 1367-4803. - ISSN 1460-2059

РУБ Biochemical Research Methods + Biotechnology & Applied Microbiology

Аннотация: Motivation: Bacterial luciferases are heterodimeric enzymes that catalyze a chemical reaction, so called bioluminescence, which causes light emission in bacteria. Bioluminescence is vastly used as a reporter system in research tools and commercial developments. However, the details of the mechanisms that stabilize and transform the reaction intermediates as well as differences in the enzymatic kinetics amongst different bacterial luciferases remain to be elucidated. Results: Amino acid sequences alignments for 21 bacterial luciferases (both alpha- and beta-subunits) were analyzed. For alpha-subunit, containing the enzyme active center, 48 polymorphic amino acid positions were identified. According to them, the sequences fell into two distinct groups known as slow and fast based on the decay rate of the bioluminescence reaction. The differences in the enzyme active site induced by structural polymorphism are analyzed.

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Держатели документа:
Siberian Fed Univ, Lab Bioluminescent Biotechnol, Krasnoyarsk, Russia.
Inst Cell Biophys RAS, Mech Cell Genome Functioning Lab, Pushchino, Moscow Region, Russia.
Moscow Inst Phys & Technol, Dolgoprudnyi, Russia.
Inst Biophys SB RAS, Lab Photobiol, Krasnoyarsk, Russia.

Доп.точки доступа:
Deeva, Anna A.; Temlyakova, Evgenia A.; Sorokin, Anatoly A.; Nemtseva, Elena V.; Kratasyuk, Valentina A.; RFBR [16-34-00746 mol_a]; Ministry of Education and Science of the Russian Federation [1762]; state budget allocated to the fundamental research at Russian Academy of Sciences [01201351504]

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13.

Stimulation of luminescence of mycelium of luminous fungus Neonothopanus nambi by ionizing radiation [Text] / T. V. Kobzeva [et al.] // Luminescence. - 2014. - Vol. 29, Is. 7. - P703-710, DOI 10.1002/bio.2656. - Cited References: 29. - The work was supported by the Program of Siberian Branch of Russian Academy of Sciences (project no. 71), Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools (project no. NSh 2272.2012.3), the Russian Foundation for Basic Research (project no. 12-03-33082), and the Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" (grant no. 11.G34.31.0058). . - ISSN 1522-7235. - ISSN 1522-7243

РУБ Biochemistry & Molecular Biology
Рубрики:
BIOLUMINESCENCE
   COMPONENTS
   MECHANISMS
   SYSTEM
Кл.слова (ненормированные):
Higher luminous fungi -- Neonothopanus nambi -- ionizing irradiation -- reactive oxygen species -- lipid peroxidation
Аннотация: The luminescent system of higher luminous fungi is not fully understood and the enzyme/substrate pair of the light emission reaction has not been isolated. It was suggested that luminescence of fungi involves oxidase-type enzymes, and reactive oxygen species are important for fungal light production. Generation of reactive oxygen species can be stimulated by ionizing irradiation, which has not been studied for luminous fungi. We report the effect of X-irradiation on the luminescence of fungus Neonothopanus nambi. Experiments were performed withmyceliumon a home-built setup based on an X-ray tube and monochromator/photomultiplier tube. Application of X-rays does not change the emission spectrum, but after approximately 20 min of continuous irradiation, light production from unsupported mycelium starts growing and increases up to approximately five times. After peaking, its level decreases irrespective of the presence of X-irradiation. After staying at a certain level, light production collapses to zero, which is not related to the drying of the mycelium or thermal impact of radiation. The observed shape of kinetics is characteristic of a multistage and/or chain reaction. The time profile of light production must reflect the current levels of radicals present in the system and/or the activity of enzyme complexes involved in light production. Copyright (C) 2014 John Wiley & Sons, Ltd.

WOS
Держатели документа:
[Kobzeva, Tatiana V.
Melnikov, Anatoly R.
Karogodina, Tatiana Y.
Zikirin, Samat B.
Stass, Dmitri V.
Molin, Yuri N.] Inst Chem Kinet & Combust SB RAS, Novosibirsk 630090, Russia
[Melnikov, Anatoly R.
Zikirin, Samat B.
Stass, Dmitri V.] Novosibirsk State Univ, Novosibirsk 630090, Russia
[Rodicheva, Emma K.
Medvedeva, Svetlana E.
Puzyr, Alexey P.
Bondar, Vladimir S.
Gitelson, Joseph I.] Inst Biophys SB RAS, Krasnoyarsk 660036, Russia
[Rodicheva, Emma K.
Medvedeva, Svetlana E.
Puzyr, Alexey P.
Burov, Andrey A.
Bondar, Vladimir S.
Gitelson, Joseph I.] Siberian Fed Univ, Krasnoyarsk 660041, Russia
[Burov, Andrey A.] Special Design Technol Bur Nauka SB RAS, Krasnoyarsk 660049, Russia
ИБФ СО РАН
СКТБ : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kobzeva, T.V.; Melnikov, A.R.; Karogodina, T.Y.; Zikirin, S.B.; Stass, D.V.; Molin, Y.N.; Rodicheva, E.K.; Medvedeva, S.E.; Puzyr, A.P.; Burov, A.A.; Bondar, V.S.; Gitelson, J.I.; Program of Siberian Branch of Russian Academy of Sciences [71]; Council for Grants of the President of the Russian Federation for Support of Leading Scientific Schools [NSh 2272.2012.3]; Russian Foundation for Basic Research [12-03-33082]; Program of Government of Russian Federation "On the Efforts for Attracting Leading Researchers to Educational Institutions of Russia" [11.G34.31.0058]

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14.

Set of Enzymatic Bioassays for Assessment of Soil Contamination / E. M. Kolosova, O. S. Sutormin, E. N. Esimbekova [et al.] // Dokl. Biol. Sci. - 2019. - Vol. 489, Is. 1. - P165-168, DOI 10.1134/S0012496619060024 . - ISSN 1608-3105
Аннотация: A concept of the comprehensive assessment of soil contamination is proposed. According to it, the conclusion regarding the presence of toxic substances in the analyzed sample is based on the inhibition of enzymatic reactions responsible for various functions of a living organism, such as luminescence, respiration, etc. These functions are taken as test functions in classical bioassays with the use of living objects (luminous bacteria, daphnia, algae, and others). The regularities of the impact of different classes of toxicants on the activity of particular enzymes or coupled oligo-enzyme chains have been established. These enzyme reactions are selected as potential test objects: markers of contamination. Three enzyme systems with the maximal sensitivity to different classes of toxicants have been chosen for the set of enzymatic bioassays: butyrylcholinesterase, NAD(P)H:FMN-oxidoreductase + luciferase, and lactate dehydrogenase + NAD(P)H:FMN-oxidoreductase + luciferase. The possibility to use enzymes instead of living organisms in the bioassay of natural complex systems has been shown.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Kolosova, E. M.; Sutormin, O. S.; Esimbekova, E. N.; Lonshakova-Mukina, V. I.; Kratasyuk, V. A.

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15.

Self-restoration as fundamental property of CES providing their sustainability / I. I. Gitelson, A. G. Degermendzhy, E. K. Rodicheva // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1641-1648, DOI 10.1016/S0273-1177(03)00111-X . - ISSN 0273-1177
Кл.слова (ненормированные):
Biocatalysts -- Ecosystems -- Genes -- Life support systems (spacecraft) -- Radiation damage -- Cell populations -- Space flight -- space shuttle -- article -- biological model -- biomass -- cell division -- Chlorella -- cytology -- growth, development and aging -- microclimate -- radiation exposure -- radiation response -- ultraviolet radiation -- Biomass -- Cell Division -- Chlorella -- Dose-Response Relationship, Radiation -- Ecological Systems, Closed -- Life Support Systems -- Models, Biological -- Ultraviolet Rays
Аннотация: Sustainability is one of the most important criteria and evaluation of human life support systems intended for use during long space flights. The common feature of biological and physicochemical life support systems is that basically they are both catalytic. But there are two fundamental properties distinguishing biological systems: 1) they are auto-catalytic: their catalysts - enzymes of protein nature - are continuously reproduced when the system functions; 2) the program of every process performed by enzymes and the program of their reproduction are inherent in the biological system itself - in the totality of genomes of the species involved in the functioning of the ecosystem. Actually, one cell with the genome capable of the phenotypic realization is enough for the self-restoration of the function performed by the cells of this species in the ecosystem. The continuous microalgal culture of Chlorella vulgaris was taken to investigate quantitatively the process of self-restoration in unicellular algae population. Based on the data obtained, we proposed a mathematical model of the restoration process in a cell population that has suffered an acute radiation damage. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Degermendzhy, A.G.; Rodicheva, E.K.

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16.

Self-restoration as fundamental property of CES providing their sustainability [Text] / I. I. Gitelson, A. G. Degermendzhy, E. K. Rodicheva ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1641-1648, DOI 10.1016/S0273-1177(03)00111-X. - Cited References: 8 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
LIFE-SUPPORT
Аннотация: Sustainability is one of the most important criteria in the creation and evaluation of human life support systems intended for use during long space flights. The common feature of biological and physicochemical life support systems is that basically they are both catalytic. But there are two fundamental properties distinguishing biological systems: 1) they are auto-catalytic: their catalysts - enzymes of protein nature - are continuously reproduced when the system functions; 2) the program of every process performed by enzymes and the program of their reproduction are inherent in the biological system itself - in the totality of genomes of the species involved in the functioning of the ecosystem. Actually, one cell with the genome capable of the phenotypic realization is enough for the self-restoration of the function performed by the cells of this species in the ecosystem. The continuous microalgal culture of Chlorella vulgaris was taken to investigate quantitatively the process of self-restoration in unicellular algae population. Based on the data obtained, we proposed a mathematical model of the restoration process in a cell population that has suffered an acute radiation damage. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Krasnoyarsk State Univ, Inst Biophys, SB RAS, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gitelson, I.I.; Degermendzhy, A.G.; Rodicheva, E.K.; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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17.

Salinity modulates thermotolerance, energy metabolism and stress response in amphipods Gammarus lacustris / K. P. Vereshchagina [et al.] // PeerJ. - 2016. - Vol. 2016, Is. 11, DOI 10.7717/peerj.2657 . - ISSN 2167-8359
Кл.слова (ненормированные):
Adaptation -- Amphipoda -- Gammarus lacustris -- Salinity -- Thermal tolerance
Аннотация: Temperature and salinity are important abiotic factors for aquatic invertebrates. We investigated the influence of different salinity regimes on thermotolerance, energy metabolism and cellular stress defense mechanisms in amphipods Gammarus lacustris Sars from two populations. We exposed amphipods to different thermal scenarios and determined their survival as well as activity of major antioxidant enzymes (peroxidase, catalase, glutathione S-transferase) and parameters of energy metabolism (content of glucose, glycogen, ATP, ADP, AMP and lactate). Amphipods from a freshwater population were more sensitive to the thermal challenge, showing higher mortality during acute and gradual temperature change compared to their counterparts from a saline lake. A more thermotolerant population from a saline lake had high activity of antioxidant enzymes. The energy limitations of the freshwater population (indicated by low baseline glucose levels, downward shift of the critical temperature of aerobic metabolism and inability to maintain steady-state ATP levels during warming) was ob- served, possibly reflecting a trade-off between the energy demands for osmoregulation under the hypo-osmotic condition of a freshwater environment and protection against temperature stress. © 2016 Vereshchagina et al.

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Держатели документа:
Institute of Biology, Irkutsk State University, Irkutsk, Russian Federation
Baikal Research Centre, Irkutsk, Russian Federation
Institute of Biophysics SB RAS, Krasnoyarsk, Russian Federation
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute for Biological Sciences, University of Rostock, Rostock, Germany

Доп.точки доступа:
Vereshchagina, K. P.; Lubyaga, Y. A.; Shatilina, Z.; Bedulina, D.; Gurkov, A.; Axenov-Gribanov, D. V.; Baduev, B.; Kondrateva, E. S.; Gubanov, M.; Zadereev, E.; Sokolova, I.; Timofeyev, M.

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18.

Rapid biosensing tools for cancer biomarkers / R. Ranjan, E. N. Esimbekova, V. A. Kratasyuk // Biosens. Bioelectron. - 2017. - Vol. 87. - P918-930, DOI 10.1016/j.bios.2016.09.061 . - ISSN 0956-5663
Кл.слова (ненормированные):
Biosensor -- Cancer biomarker -- Functional nanomaterials -- Microfluidics -- Point-of-care devices -- Antibodies -- Biosensors -- Diseases -- Electronic properties -- Microfluidics -- Nanostructured materials -- Cancer biomarkers -- Diagnostic systems -- Fluorescent probes -- Functional Nano materials -- Latest development -- Optoelectronic properties -- Point of care -- Rapid bio-sensing -- Biomarkers
Аннотация: The present review critically discusses the latest developments in the field of smart diagnostic systems for cancer biomarkers. A wide coverage of recent biosensing approaches involving aptamers, enzymes, DNA probes, fluorescent probes, interacting proteins and antibodies in vicinity to transducers such as electrochemical, optical and piezoelectric is presented. Recent advanced developments in biosensing approaches for cancer biomarker owes much credit to functionalized nanomaterials due to their unique opto-electronic properties and enhanced surface to volume ratio. Biosensing methods for a plenty of cancer biomarkers has been summarized emphasizing the key principles involved. © 2016 Elsevier B.V.

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Держатели документа:
Laboratory of Bioluminescent Biotechnologies, Department of Biophysics, Institute of Fundamental Biology and Biotechnology, Siberian Federal University, 79 Svobodny prospect, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, Akademgorodok 50/50, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Ranjan, R.; Esimbekova, E. N.; Kratasyuk, V. A.

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19.

Rapid biosensing tools for cancer biomarkers / R. Ranjan, E. N. Esimbekova, V. A. Kratasyuk // Biosens. Bioelectron. - 2017. - Vol. 87. - P918-930, DOI 10.1016/j.bios.2016.09.061. - Cited References:115. - The research was partially supported by the Russian Foundation for Basic Research (Project no. 16-34-60100 and No. 16-06-00439), the state budget allocated to the fundamental research Russian Academy of Sciences (Project no. 01201351504). . - ISSN 0956-5663. - ISSN 1873-4235

РУБ Biophysics + Biotechnology & Applied Microbiology + Chemistry, Analytical
Рубрики:
POINT-OF-CARE
   HIGHLY SENSITIVE DETECTION
   ACOUSTIC-WAVE BIOSENSOR
   DNA
Кл.слова (ненормированные):
Biosensor -- Cancer biomarker -- Functional nanomaterials -- Point-of-care -- devices -- Microfluidics
Аннотация: The present review critically discusses the latest developments in the field of smart diagnostic systems for cancer biomarkers. A wide coverage of recent biosensing approaches involving aptamers, enzymes, DNA probes, fluorescent probes, interacting proteins and antibodies in vicinity to transducers such as electrochemical, optical and piezoelectric is presented. Recent advanced developments in biosensing approaches for cancer biomarker owes much credit to functionalized nanomaterials due to their unique opto-electronic properties and enhanced surface to volume ratio. Biosensing methods for a plenty of cancer biomarkers has been summarized emphasizing the key principles involved.

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Держатели документа:
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Dept Biophys, Lab Bioluminescent Biotechnol, Krasnoyarsk 660041, Russia.
Inst Biophys SB RAS, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Ranjan, Rajeev; Esimbekova, Elena N.; Kratasyuk, Valentina A.; Russian Foundation for Basic Research [16-34-60100, 16-06-00439]; state budget allocated to the fundamental research Russian Academy of Sciences Project [01201351504]

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20.

Protein-based fluorescent bioassay for low-dose gamma radiation exposures / A. S. Petrova [et al.] // Anal. Bioanal. Chem. - 2018, DOI 10.1007/s00216-018-1282-5 . - ISSN 1618-2642
Кл.слова (ненормированные):
Bioassay -- Enzymes -- Fluorescence/luminescence -- Fluorescent protein -- Gamma radiation -- Radiotoxicity -- Efficiency -- Enzymes -- Fluorescence -- Gamma rays -- Proteins -- Proton transfer -- Fluorescence characteristics -- Fluorescence intensities -- Fluorescence spectra -- Fluorescence/luminescence -- Fluorescent protein -- Photochemical process -- Physiological liquids -- Radiotoxicity -- Bioassay
Аннотация: The study suggests an application of a coelenteramide-containing fluorescent protein (CLM-CFP) as a simplest bioassay for gamma radiation exposures. “Discharged obelin,” a product of the bioluminescence reaction of the marine coelenterate Obelia longissima, was used as a representative of the CLM-CFP group. The bioassay is based on a simple enzymatic reaction—photochemical proton transfer in the coelenteramide-apoprotein complex. Components of this reaction differ in fluorescence color, providing, by this, an evaluation of the proton transfer efficiency in the photochemical process. This efficiency depends on the microenvironment of the coelenteramide within the protein complex, and, hence, can evaluate a destructive ability of gamma radiation. The CLM-CFP samples were exposed to gamma radiation (137Cs, 2 mGy/h) for 7 and 16 days at 20 °C and 5 °C, respectively. As a result, two fluorescence characteristics (overall fluorescence intensity and contributions of color components to the fluorescence spectra) were identified as bioassay parameters. Both parameters demonstrated high sensitivity of the CLM-CFP-based bioassay to the low-dose gamma radiation exposure (up to 100 mGy). Higher temperature (20 °C) enhanced the response of CLM-CFP to gamma radiation. This new bioassay can provide fluorescent multicolor assessment of protein destruction in cells and physiological liquids under exposure to low doses of gamma radiation. [Figure not available: see fulltext.]. © 2018, Springer-Verlag GmbH Germany, part of Springer Nature.

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Держатели документа:
Krasnoyarsk State Agrarian University, Mira Avenue 90, Krasnoyarsk, Russian Federation
Siberian Federal University, Svobodnyy Ave 79, Krasnoyarsk, Russian Federation
Institute of Biophysics SB RAS, FRC KSC SB RAS, Krasnoyarsk, Russian Federation
Department of Radiology, University of Pennsylvania, 3401 N Broad St., Philadelphia, PA, United States

Доп.точки доступа:
Petrova, A. S.; Lukonina, A. A.; Dementyev, D. V.; Bolsunovsky, A. Ya. ; Popov, A. V.; Kudryasheva, N. S.

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