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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Кратасюк В.А., Гительзон И.И.
Заглавие : Использование светящихся бактерий в биолюминесцентном анализе : научное издание
Место публикации : Успехи микробиол. - Москва, 1987. - N 21. - С. 3-30. - ISSN 0566-392X
ГРНТИ : 34.15.33
Предметные рубрики: БАКТЕРИИ
СВЕТЯЩИЕСЯ КУЛЬТУРЫ
СВОЙСТВА
ИММОБИЛИЗОВАННЫЙ ФЕРМЕНТ
ПОЛУЧЕНИЕ
ПРИМЕНЕНИЕ
БИОЛЮМИНЕСЦЕНТНЫЙ АНАЛИЗ
ОБЩИЕ ПРИНЦИПЫ
ОБЗОРЫ
БИБЛ 161
IMMOBILIZED ENZYMES
В О М Е СЕ СЕ
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Петушков В.Н.
Заглавие : Биолюминесцентный анализ ферментных систем : научное издание
Место публикации : Молекул. механизмы клеточ. гомеостаза. Матер. шк., Красноярск, 13-22 марта, 1984. - Новосибирск, 1987. - С. 204-216
ГРНТИ : 34.17.05
Предметные рубрики: ФЕРМЕНТ
АКТИВНОСТЬ
ИЗМЕРЕНИЕ
БИОЛЮМИНЕСЦЕНЦИЯ
ЛЮЦИФЕРАЗА
ПРИМЕНЕНИЕ
ENZYMES
В О М Е СЕ СЕ
С Е А А
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GITELZON I.I., SANDALOVA T.P.
Заглавие : PROSPECTS FOR APPLICATION OF BIOLUMINESCENCE METHOD IN MEDICINE
Колич.характеристики :5 с
Место публикации : VESTNIK AKADEMII MEDITSINSKIKH NAUK SSSR: IZD VO MEDITSINA, 1990. - Is. 9. - С. 31-35. - ISSN 0002-3027
Примечания : Cited References: 41
Предметные рубрики: AMINO-ACID SEQUENCE
NUCLEOTIDE-SEQUENCE
VIBRIO-HARVEYI
BACTERIAL LUCIFERASE
FIREFLY LUCIFERASE
SUBUNIT
CELLS
GENE
PHOTOPROTEINS
EXPRESSION
Аннотация: Major advances in the development and application of the bioluminescent analysis to detect certain biologically active substances are discussed. The main merit of the method lies in its high sensitivity and specificity along with its simplicity and rapid performance. The available methodologies allow for detection of substances of varying nature: Ca2+, ATP, FMN, NAD(P), long-chain aldehydes, ATP- and NAD(P)-dependent enzymes and their substrates, many xenobiotics and antibiotics, and mutagens. The bioluminescence methodologies may be widely applied in clinical laboratory diagnosis.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Бондарь В.С., Высоцкий Е.С., Летунов В.Н.
Заглавие : Получение высокоочищенной глутатионредуктазы из гидроидных полипов Obelia longissima и изучение некоторых ее физико-химических свойств : научное издание
Коллективы : Академия наук СССР. Сибирское отделение. Институт биофизики
Место публикации : Препр. - 1990. - N 123. - С. 1-32
ГРНТИ : 31.27.17
Предметные рубрики: ГЛУТАТИОНРЕДУКТАЗА
ВЫСОКООЧИЩЕННАЯ
ПОЛУЧЕНИЕ
СВОЙСТВА
ПОЛИПЫ ГИДРОИДНЫЕ
OBELIA LONGISSIMA
ENZYMES
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : SANDALOVA T.P., TYULKOVA N.A.
Заглавие : INACTIVATION OF BACTERIAL LUCIFERASES BY N-ETHYLMALEIMIDE
Колич.характеристики :7 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1992. - Vol. 57, Is. 6. - P552-558. - ISSN 0006-2979
Примечания : Cited References: 21
Предметные рубрики: AMINO-ACID SEQUENCE
NUCLEOTIDE-SEQUENCE
REACTIVE SULFHYDRYL
PHOTOBACTERIUM-LEIOGNATHI
VIBRIO-HARVEYI
BIOLUMINESCENCE
SUBUNIT
REGION
GENE
Ключевые слова (''Своб.индексиров.''): luciferase--n-ethylmaleimide
Аннотация: The kinetics of inactivation of luciferases from four species of luminescent bacteria by the thiol reagent N-ethylmaleimide were investigated The dependencies of inactivation on ionic strength differed among the enzymes. Increasing the molarity of the buffer increased the rate of inactivation of all luciferases except that of Vibrio harveyi. Modification of Photobacterium phosphoreum luciferase decreased the maximal intensity of bioluminescence, whereas modification of Photobacterium leiognathi and Vibrio fischeri luciferases in high ionic strength buffers decreased the maximal intensity of bioluminescence and changed the luminescence decay rate constant. High ionic strength apparently alters the conformational states of the luciferases.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : KRATASYUK V.A., ABAKUMOVA V.V., KIM N.B.
Заглавие : A GEL MODEL FOR THE FUNCTIONING OF LUCIFERASE IN THE CELL
Колич.характеристики :5 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1994. - Vol. 59, Is. 7. - P761-765. - ISSN 0006-2979
Примечания : Cited References: 11
Предметные рубрики: BIOLUMINESCENT
Ключевые слова (''Своб.индексиров.''): bioluminescence--luciferase--nadh, fmn-oxidoreductase--immobilization
Аннотация: A gel model for the functioning of luciferase in cells has been constructed using bacterial NADH:FMN-oxidoreductase and luciferase immobilized in starch gel disks. The characteristics of the immobilized luciferase depend on the duration of drying, the amount and concentration of the gel, the nature of the support used for drying, and the properties of the initial enzyme preparation. Functionally important enzyme groups remain intact in the immobilized preparation, and luciferase retains its high specificity with respect to aldehydes. The gel microenvironment appears to be optimal for luciferase, judging from its high activity and increased stability. Conditions allowing repeated use of the preparation have been found. The approach permits co-immobilization of luciferase with other enzymes and their substrates. The error in bioluminescence measurements using the disks is 5-10%. A procedure for stabilization of the immobilized luciferase during repeated use has been devised.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Tyulkova N.A., Sandalova T.P.
Заглавие : Comparative study of temperature effects on bacterial luciferases
Колич.характеристики :10 с
Место публикации : Biochem.-Moscow: PLENUM PUBL CORP, 1996. - Vol. 61, Is. 2. - P205-214. - ISSN 0006-2979
Примечания : Cited References: 23
Предметные рубрики: BIOLUMINESCENCE
Ключевые слова (''Своб.индексиров.''): bacterial luciferase--temperature--activation energy
Аннотация: Effects of temperature on bioluminescent patterns of luciferases from luminescent bacteria Vibrio harveyi, Vibrio fischeri, Photobacterium leiognathi, and Photobacterium phosphoreum were studied. The highest luminescence level was observed at 15-25 degrees C for the luciferase from P. phosphoreum, at 20-30 degrees C for the V. fischeri and P. leiognathi enzymes, and at 30-37 degrees C for the enzyme from V. harveyi. All the luciferases were significantly stabilized at increased salt concentrations, at low pH values, or in the presence of dithiothreitol (DTT) and EDTA. The addition of DTT and EDTA affected the reversible stage of enzyme inactivation, while salts reduced the rate of the irreversible stage. A peak corresponding to aggregated protein was detected by gel chromatography of irreversibly inactivated luciferase. Activation energies were calculated for each luciferase in bioluminescent reactions with decanal, dodecanal, tetradecanal, and without aldehydes. The activation energy of the reaction with tetradecanal was much lower than those with the other aldehydes. The temperature dependence of the lifetime of the long-lived reaction intermediate showed that in the 10-30 degrees C interval all the luciferases, except for the enzyme from V. harveyi, have only one active form.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bondar V.S., Boersma M.G., Golovlev E.L., Vervoort J..., Van Berkel WJH, Finkelstein Z.I., Solyanikova I.P., Golovleva L.A., Rietjens IMCM
Заглавие : (19)F NMR study on the biodegradation of fluorophenols by various Rhodococcus species
Колич.характеристики :12 с
Место публикации : Biodegradation: SPRINGER, 1998. - Vol. 9: NMR in Environmental Sciences Symposium (OCT 24, 1997, EDE, NETHERLANDS), Is. 6. - С. 475-486. - ISSN 0923-9820, DOI 10.1023/A:1008391906885
Примечания : Cited References: 37
Предметные рубрики: NUCLEAR-MAGNETIC-RESONANCE
PSEUDOMONAS-PUTIDA MT-2
PHENOL HYDROXYLASE
BACTERIAL-DEGRADATION
3-OXOADIPATE PATHWAY
FLUOROBENZOIC ACID
CONVERSION
METABOLISM
CYCLOISOMERASES
2-CHLORO-CIS,CIS-MUCONATE
Ключевые слова (''Своб.индексиров.''): biodegradation--fluorophenols--(19)f nmr--oxidative defluorination--rhodococcus species
Аннотация: Of all NMR observable isotopes (19)F is the one perhaps most convenient for studies on biodegradation of environmental pollutants. The reasons underlying this potential of (19)F NMR are discussed and illustrated on the basis of a study on the biodegradation of fluorophenols by four Rhodococcus strains. The results indicate marked differences between the biodegradation pathways of fluorophenols among the various Rhodococcus species. This holds not only for the level and nature of the fluorinated biodegradation pathway intermediates that accumulate, but also for the regioselectivity of the initial hydroxylation step. Several of the Rhodococcus species contain a phenol hydroxylase that catalyses the oxidative defluorination of ortho-fluorinated di- and trifluorophenols. Furthermore, it is illustrated how the (19)F NMR technique can be used as a tool in the process of identification of an accumulated unknown metabolite, in this case most likely 5-fluoromaleylacetate. Altogether, the (19)F NMR technique proved valid to obtain detailed information on the microbial biodegradation pathways of fluorinated organics, but also to provide information on the specificity of enzymes generally considered unstable and, for this reason, not much studied so far.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N..., Kratasyuk V..., Esimbekova E..., Vetrova E..., Nemtseva E..., Kudinova I...
Заглавие : Development of bioluminescent bioindicators for analysis of environmental pollution
Колич.характеристики :4 с
Место публикации : Field Anal. Chem. Technol.: JOHN WILEY & SONS INC, 1998. - Vol. 2, Is. 5. - P277-280. - ISSN 1086-900X, DOI 10.1002/(SICI)1520-6521(1998)2:5277::AID-FACT43.0.CO;2-P
Примечания : Cited References: 17
Ключевые слова (''Своб.индексиров.''): biotest--bioluminescence--enzymes--environmental monitoring
Аннотация: The influence of several suites of pollutants (metallic salts, quinones, and phenols) on bacterial bioluminescence in vivo and in vitro (five test systems) was investigated. The sensitivity of bioluminescence to the different pollutants was evaluated, and inhibition constants were measured. The data obtained were shown to correlate with the physical and chemical characteristics of the substances and the structure of the bioluminescent systems. It has been found that three bioluminescent tests (water-soluble enzyme systems, immobilized enzyme systems, and bioluminescent bacteria) show higher sensitivity to pollutants and cover all types of widespread contamination. These tests were chosen as a set of bioluminescent assays for the detection of pollutants. (C) 1998 John Wiley & Sons, Inc.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kratasyuk V.A., Kudinova I.Y.
Заглавие : Practical enzymology course based on bioluminescence
Колич.характеристики :4 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 1999. - Vol. 14: 10th International Symposium on Bioluminescence and Chemiluminescence (1998, BOLOGNA, ITALY), Is. 4. - P189-192. - ISSN 1522-7235, DOI 10.1002/(SICI)1522-7243(199907/08)14:4189::AID-BIO5273.0.CO;2-E
Примечания : Cited References: 7
Ключевые слова (''Своб.индексиров.''): enzyme--science education--luciferase--bioluminescence
Аннотация: We describe our experience with laboratory courses in enzymology based on the phenomenon of bioluminescence. The soluble and immobilized enzymes of luminous bacteria are used and the practical enzymological course consists of four main courses: (1) training in measuring the activities of soluble and immobilized enzymes; (2) the investigation of kinetic characteristics (kinetic constants) and enzyme-substrate and enzyme-inhibitor interactions in the bacterial bioluminescent reaction; (3) The testing of physico-chemical characteristics of enzymes (pH, temperature, ion strength, etc.); (4) the effect of inhibitors on enzymes. Training is possible in groups of about ten persons. Our practice work has been introduced in the biological, pedagogical and physical departments of Krasnoyarsk State University. Students of the pedagogical department have created a popular and interesting series of laboratory works for high school children aged 14-17 years. Copyright (C) 1999 John Wiley & Sons, Ltd.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kudryasheva N.S., Esimbekova E.N., Kudinova I.Y., Kratasyuk V.A., Stom D.I.
Заглавие : Effects of quinones on NADH-dependent enzymatic bioluminescent systems
Колич.характеристики :5 с
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA, 2000. - Vol. 36, Is. 4. - P409-413. - ISSN 0003-6838, DOI 10.1007/BF02738052
Примечания : Cited References: 13
Аннотация: The effects of a number of quinones on the bioluminescence characteristics of a three-component enzymatic system containing alcohol dehydrogenase, bacterial luciferase, and NADH-FMN oxidoreductase were studied to find the most sensitive kinetic parameters of the system intended to be used in biological testing. Both direct and back reactions catalyzed by alcohol dehydrogenase were studied in the presence and in the absence of quinones. The kinetic parameters of the bioluminescent system were found to depend on the redox potentials and concentrations of quinones. The quinone-induced effects were shown to be associated with changes in the NAD(+)/NADH ratio in the chain of NADH-dependent enzymes, The three-enzyme system based on alcohol dehydrogenase is suggested as a bioluminescence test for ecological monitoring of waste water.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sukovataya I.E., Tyulkova N.A.
Заглавие : Kinetic analysis of bacterial water-organic media
Колич.характеристики :3 с
Место публикации : Luminescence: JOHN WILEY & SONS LTD, 2001. - Vol. 16: 11th International Bioluminescence and Chemiluminescence Symposium (SEP 06-10, 2000, PACIFIC GROVE, CALIFORNIA), Is. 4. - P271-273. - ISSN 1522-7235, DOI 10.1002/bio.649.abs
Примечания : Cited References: 10
Ключевые слова (''Своб.индексиров.''): bacterial luciferase--organic solvents--michaelis constant
Аннотация: The interaction of luciferases from two types of luminous bacteria, Photobacterium leiognathi and Vibrio harveyi, with their substrates [the photorecovered. FMNH2 and long-chain aldehydes-decanal (C-10), dodecanal (C-12) and tetradecanal (C-14)] in water-organic media was analysed using kinetic graphical methods. Moderate concentrations of organic solvents have been demonstrated to activate the bioluminescence, while higher concentrations inhibit it. The interactions of these effectors with luciferases show different types of kinetics, which depend on concentrations of solvents, kinds of enzymes and substrates. The apparent value of the Michaelis constant, K-m for C-14 of both luciferases and for C-10 of luciferase V. harveyi is enhanced with increasing concentration of the organic solvent, but K-m for C-12 and C-10 of luciferase P. leiognathi decreases. Obviously, at the specific binding of aldehydes with luciferases in the first case, hydrophobic interactions are realized, but in second, the electrostatic interactions are realized. The series of changes in parameters of bioluminescence reaction catalysed by different luciferases is obviously determined by their structural peculiarities. Copyright (C) 2001 John Wiley & Sons, Ltd.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide
Колич.характеристики :7 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 2001. - Vol. 70, Is. 6. - P640-646. - ISSN 0026-2617, DOI 10.1023/A:1013175413013
Примечания : Cited References: 19
Ключевые слова (''Своб.индексиров.''): hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis--co inhibition
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-beta-ketothiolase. acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % beta-hydroxybutyrate and trace amounts of beta-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by Alcaligenes eutrophus in the presence of carbon monoxide
Место публикации : Microbiology. - 2001. - Vol. 70, Is. 6. - С. 640-646. - ISSN 00262617 (ISSN)
Ключевые слова (''Своб.индексиров.''): co inhibition--hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen. В© 2001 MAIK "Nauka/Interperiodica".
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : The autotrophic synthesis of polyhydroxyalkanoate by alcaligenes eutrophus in the presence of carbon monoxide
Место публикации : Mikrobiologiya. - 2001. - Vol. 70, Is. 6. - С. 745-752. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): co inhibition--hydrogen-oxidizing bacteria--polyhydroxyalkanoates (phas)--synthesis--alkane--carbon monoxide--fatty acid--hydroxyacid--lipid--nitrogen--polyester--article--chemistry--culture medium--drug antagonism--growth, development and aging--mass spectrometry--metabolism--wautersia eutropha--alkanes--carbon monoxide--culture media--cupriavidus necator--fatty acids--hydroxy acids--lipids--mass spectrometry--nitrogen--polyesters
Аннотация: The CO-resistant strain B5786 of the hydrogen-oxidizing bacterium Alcaligenes eutrophus was found to be able to synthesize polyhydroxyalkanoates (PHAs) under the conditions of growth limitation by nitrogen deficiency (the factor that promotes PHA synthesis) and growth inhibition by carbon monoxide. The gas mixtures that contained from 5 to 20 vol % CO did not inhibit the key enzymes of PHA synthesis-?-ketothiolase, acetoacetyl-CoA reductase, hydroxybutyrate dehydrogenase, and PHA synthase. In the presence of CO, cells accumulated up to 70-75 wt % PHA (with respect to the dry biomass) without any noticeable increase in the consumption of the gas substrate. Chromatographic-mass spectrometric analysis showed that the PHA synthesized by A. eutrophus is a copolymer containing more than 99 mol % ?-hydroxybutyrate and trace amounts of ?-hydroxyvalerate. The PHA synthesized under the conditions described did not differ from that synthesized by A. eutrophus cells from electrolytic hydrogen.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide
Место публикации : Applied Microbiology and Biotechnology. - 2002. - Vol. 58, Is. 5. - С. 675-678. - ISSN 01757598 (ISSN) , DOI 10.1007/s00253-002-0941-8
Ключевые слова (''Своб.индексиров.''): 3 hydroxybutyric acid--acetoacetyl coenzyme a reductase--acetyl coenzyme a acyltransferase--beta hydroxyvalerate--butyrate dehydrogenase--carbon monoxide--electrolyte--hydrogen--oxidoreductase--poly(3 hydroxybutyric acid)--poly(3 hydroxybutyric acid)synthase--polyhydroxyalkanoic acid--polymer--unclassified drug--valeric acid--bacterium--article--autotrophy--bacterial growth--bacterial strain--biomass production--controlled study--crystallization--enzyme activity--molecular weight--nonhuman--synthesis--temperature--wautersia eutropha--carbon monoxide--culture media--cupriavidus necator--fatty acids--lipids--polyesters--bacteria (microorganisms)--negibacteria--ralstonia--wautersia eutropha
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxy-alkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO (v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (?-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly ?-hydroxybutyrate (more than 99 mol%) with trace amounts of ?-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide
Колич.характеристики :4 с
Место публикации : Appl. Microbiol. Biotechnol.: SPRINGER-VERLAG, 2002. - Vol. 58, Is. 5. - P675-678. - ISSN 0175-7598, DOI 10.1007/s00253-002-0941-8
Примечания : Cited References: 35
Предметные рубрики: PSEUDOMONAS-OLEOVORANS
ALCALIGENES-EUTROPHUS
COAL
CULTIVATION
METABOLISM
SUBSTRATE
CULTURE
CO2
H-2
O-2
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxyalkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (beta-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly beta-hydroxybutyrate (more than 99 mol%) with trace amounts of beta-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Popova T.G.
Заглавие : Study of the biological properties of polyoxyalkanoates in the in vivo chronic experiment
Место публикации : Meditsinskaya Tekhnika. - 2002. - Is. 4. - С. 29-33. - ISSN 00258075 (ISSN)
Ключевые слова (''Своб.индексиров.''): biomaterial--polymer--animal--article--bioremediation--comparative study--female--metabolism--rat--suture--wistar rat--animals--biocompatible materials--biodegradation, environmental--female--polymers--rats--rats, wistar--sutures--biocompatibility--immunology--implants (surgical)--materials testing--organic polymers--polyoxyalkanoates--rat models--suture threads--biomaterials
Аннотация: Chronic experiments on mature Wistar rats studied the biological properties of suture threads from two types of polyoxyalkanoates (POA), such as polyoxybutyrate (POB) and the copolymer of POB and polyoxyvalerate (POV) (POB/POV) as compared to the conventional surgical material (silk and catgut). The physiological and biochemical parameters that reflect the status of the animals, including their behavior, height and development, the weight of the body and viscera, the morphology of peripheral blood, the biochemical parameters of blood, the activity of some enzymes, as well as histological findings of the viscera, and lymphoid tissue responses were assessed. POA was found to produce no negative effect on the physiological, biochemical, and functional characteristics of the animals irrespective of the duration of contact with the internal medium of the body and of the chemical composition of a material.
Scopus
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gitelson I.I., Degermendzhy A.G., Rodicheva E.K.
Заглавие : Self-restoration as fundamental property of CES providing their sustainability
Место публикации : Advances in Space Research. - 2003. - Vol. 31, Is. 7. - С. 1641-1648. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(03)00111-X
Ключевые слова (''Своб.индексиров.''): biocatalysts--ecosystems--genes--life support systems (spacecraft)--radiation damage--cell populations--space flight--space shuttle--article--biological model--biomass--cell division--chlorella--cytology--growth, development and aging--microclimate--radiation exposure--radiation response--ultraviolet radiation--biomass--cell division--chlorella--dose-response relationship, radiation--ecological systems, closed--life support systems--models, biological--ultraviolet rays
Аннотация: Sustainability is one of the most important criteria and evaluation of human life support systems intended for use during long space flights. The common feature of biological and physicochemical life support systems is that basically they are both catalytic. But there are two fundamental properties distinguishing biological systems: 1) they are auto-catalytic: their catalysts - enzymes of protein nature - are continuously reproduced when the system functions; 2) the program of every process performed by enzymes and the program of their reproduction are inherent in the biological system itself - in the totality of genomes of the species involved in the functioning of the ecosystem. Actually, one cell with the genome capable of the phenotypic realization is enough for the self-restoration of the function performed by the cells of this species in the ecosystem. The continuous microalgal culture of Chlorella vulgaris was taken to investigate quantitatively the process of self-restoration in unicellular algae population. Based on the data obtained, we proposed a mathematical model of the restoration process in a cell population that has suffered an acute radiation damage. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
Scopus
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20.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Gitelson I.I., Degermendzhy A.G., Rodicheva E.K.
Заглавие : Self-restoration as fundamental property of CES providing their sustainability
Колич.характеристики :8 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P1641-1648. - ISBN 0273-1177, DOI 10.1016/S0273-1177(03)00111-X
Примечания : Cited References: 8
Предметные рубрики: LIFE-SUPPORT
Аннотация: Sustainability is one of the most important criteria in the creation and evaluation of human life support systems intended for use during long space flights. The common feature of biological and physicochemical life support systems is that basically they are both catalytic. But there are two fundamental properties distinguishing biological systems: 1) they are auto-catalytic: their catalysts - enzymes of protein nature - are continuously reproduced when the system functions; 2) the program of every process performed by enzymes and the program of their reproduction are inherent in the biological system itself - in the totality of genomes of the species involved in the functioning of the ecosystem. Actually, one cell with the genome capable of the phenotypic realization is enough for the self-restoration of the function performed by the cells of this species in the ecosystem. The continuous microalgal culture of Chlorella vulgaris was taken to investigate quantitatively the process of self-restoration in unicellular algae population. Based on the data obtained, we proposed a mathematical model of the restoration process in a cell population that has suffered an acute radiation damage. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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