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^a343.17.09.11^2VINITI
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Взаимодействие бактериальной люцефаразы с амфифильными молекулами в растворе, на поверхности воды и в пленках Ленгмюра-Блоджетт [Текст] : научное издание / П. И. Белобров [и др.] ; АН СССР. СО. Ин-т физ. // Препр. - 1988. - N 92Б. - С. 1-28

ГРНТИ

34.17.09

РУБ 343.17.09.11
Рубрики:
БИОЛЮМИНЕСЦЕНЦИЯ
   ЛЮЦИФЕРАЗА
   СВОЙСТВА
   БИОСЕНСОРЫ
   BACTERIA LUCIFERASE
   Р ОРЕ Т Е III SOLUTION WATER FILMS
   В О Е О
Аннотация: Исследованы амфифильные свойства бакт. люциферазы в различных условиях и ее взаимодействие с длинноцепоч. алифатич. альдегидом и соответствующей жирной к-той. Методом малоуглового рассеяния рентген. лучей определены радиусы инерции фермента в р-ре и найдены зависимости их от состояния люциферазы. Исследована реология монослоев, полученных из белка, субстрата, продукта и их комбинаций со строго определенными соотношениями на поверхности различных субфаз. Соответствующие 'пи'-А изотермы показывают, что во всех случаях образуются твердые двумерные пленки, сжимаемость к-рых сильно зависит от соотношения компонент. Монослои перенесены на твердую подложку и получены мультислойные пленки на разных носителях. Рентгеновское исследование позволило определить ближний порядок в толстых пленках, к-рый также зависит от соотношения компонент. Метод позволяет конструировать регулярные неоднородности распределения люциферазы в монослое. Результаты электр. микроскопии чистых и смешанных ионо- и бислоев, перенесенных на твердые подложки, свидетельствуют в пользу микроэмульсионной модели амфифильности люциферазы. Получены ленгмюровские пленки с сохранением активности. Это открывает путь к новому поколению люциферазных твердотельных биосенсоров. Библ. 21
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Белобров, П.И.; Воеводин, В.А.; Ерохин, В.В.; Львов, Ю.М.; Петушков, В.Н.; Пузырь, А.П.; Родионова, Н.С.

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ACCUMULATION OF POLYHYDROXYBUTYRATE BY THE AEROBIC CO-OXIDIZING BACTERIUM SELIBERIA-CARBOXYDOHYDROGENA [Text] / T. G. VOLOVA, G. S. KALACHEVA, V. M. KONSTANTINOVA // Microbiology. - 1994. - Vol. 63, Is. 2. - P109-112. - Cited References: 20 . - 4. - ISSN 0026-2617

РУБ Microbiology

Аннотация: The synthesis of polyhydroxybutyrate by the carboxydobacterium Seliberia carboxydohydrogena under various growth conditions was studied. The bacterium was able to accumulate poly-beta-hydroxybutyric acid and a heteropolymer that was a product of copolymerization of hydroxybutyric and hydroxyvaleric acids. Nitrogen and sulfur exerted the strongest effect on polymer accumulation in carboxydobacteria: The maximum polymer concentrations in cells reached 28%. Specimens of polyhydroxybutyrate films were obtained, and their degradability by soil microflora was shown.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
VOLOVA, T.G.; KALACHEVA, G.S.; KONSTANTINOVA, V.M.

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The influence of a monolayer of an aliphatic acid on temperature oscillation in the surface layer of water / M. I. Gladyshev, L. A. Levin, N. N. Sushchik // Izvestiya - Atmospheric and Ocean Physics. - 1996. - Vol. 32, Is. 3. - P378-380 . - ISSN 0001-4338
Кл.слова (ненормированные):
aliphatic acid -- surface films -- temperature -- thermal spots
Аннотация: The influence of a monolayer of a palmitic acid on amplitude-frequency characteristics of temperature oscillations caused by thermal spots of a surface film was experimentally determined in a Langmuir tank. An analysis of the power spectra showed that the temperature oscillations were multimodal. In addition, no consistant differences between the spectrum for a pure surface and the spectrum for a surface covered by an acid monolayer were found. A roughened gain-frequency factor was found that is equal to the ratio of the average period between the thermal spots to the dispersion of the time series of temperature oscillations. The factor significantly differed over a wide range of temperatures for a pure surface and for a surface covered with the monolayer. The experimental results do not confirm the existence of a leading frequency in the temperature oscillations of a cold surface film due to an appearance of thermal spots.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Levin, L.A.; Sushchik, N.N.

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Studies of Biodegradation of Microbial Polyhydroxyalkanoates / T. G. Volova [и др.] // Prikladnaya Biokhimiya i Mikrobiologiya. - 1998. - Vol. 34, Is. 5. - С. 543 . - ISSN 0555-1099
Аннотация: Biodegradation of polyhydroxyalkanoates (PHA) - polymers of hydroxylated fatty acids - differing in chemical composition, crystallinity, melting temperature, and molecular weight was studied in soil and water environments under conditions of varying ambient temperature and pH. Under similar conditions, homogeneous films of polyhydroxybutyrate (PHB) with different initial properties were degraded at virtually equal rates. Hydroxybutyrate-hydroxyvalerate copolymers with a lower crystallinity and different microstructure in comparison to PHB were degraded at a 20 to 30% higher rate on average. The ambient temperature significantly influenced the rate of PHA biodegradation.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Belyaeva, O.G.; Plotnikov, V.F.; Puzyr', A.P.

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Studies of biodegradation of microbial polyhydroxyalkanoates / T. G. Volova [et al.] // Applied Biochemistry and Microbiology. - 1998. - Vol. 34, Is. 5. - P488-492 . - ISSN 0003-6838
Аннотация: Biodegradation of polyhydroxyalkanoates (PHA) - polymers of hydroxylated fatty acids - differing in chemical composition, crystallinity, melting temperature, and molecular weight was studied in soil and water environments under conditions of varying ambient temperature and pH. Under similar conditions, homogeneous films of polyhydroxybutyrate (PHB) with different initial properties were degraded at virtually equal rates. Hydroxybutyrate-hydroxyvalerate copolymers with a lower crystallinity and different microstructure in comparison to PHB were degraded at a 20 to 30% higher rate on average. The ambient temperature significantly influenced the rate of PHA biodegradation.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk, 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Belyaeva, O.G.; Plotnikov, V.F.; Puzyr', A.P.

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Surface properties of nanodiamond films deposited by electrophoresis on Si(100) / E. Maillard-Schaller [et al.] // Diamond and Related Materials. - 1999. - Vol. 8, Is. 2-5. - P805-808 . - ISSN 0925-9635
Кл.слова (ненормированные):
Energy band diagram -- Nanodiamond -- Raman spectroscopy -- Surface characterization -- Band structure -- Electrodeposition -- Electrophoresis -- Hydrogen -- Nanostructured materials -- Nitrogen -- Oxidation -- Oxygen -- Phonons -- Plasma applications -- Silicon wafers -- Surface properties -- Dielectrophoresis -- Negative electron affinity (NEA) -- Phonon confinement effect -- Diamond films
Аннотация: The surface properties of diamond nanoparticles (40-50 A in diameter) have been investigated by X-ray photoelectron spectroscopy (XPS), UV photoelectron spectroscopy (UPS) and Raman spectroscopy. The diamond nanoparticles have been deposited on flat Si(100) substrates by electrophoresis/dielectrophoresis. The as-deposited films are strongly oxidized and present a 1-2% nitrogen content. After treatment at 850 В°C in H2 plasma for 60 min, the oxygen is removed, and the position of the C 1s core-level peak indicates a n-type electronic comportment of the diamond nanoparticles. Raman spectroscopy of the as-deposited film shows a sp3 contribution at 1321 cm-1 and a sp2 contribution around 1620 cm-1. The 12 cm-1 shift of the sp3 contribution with respect to the bulk diamond peak at 1333 cm-1 is attributed to a phonon confinement effect due to the size of the diamond particles. The H2 plasma treatment induces a size decrease of the nanocrystallites confirmed by Raman and scanning electron microscopy (SEM) measurements. UPS spectroscopy shows a negative electron affinity of -0.2 eV of the hydrogenated nanodiamond film.

Scopus
Держатели документа:
Solid State Physics Department, University of Fribourg, 1700, Fribourg, Switzerland
Institute of Christallography, 117333, Moscow, Russian Federation
Institute of Biophysics, 660036, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Maillard-Schaller, E.; Kuettel, O.M.; Diederich, L.; Schlapbach, L.; Zhirnov, V.V.; Belobrov, P.I.

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Characterization of field emission cathodes with different forms of diamond coatings [Text] / V. V. Zhirnov [et al.] // J. Vac. Sci. Technol. B. - 1999. - Vol. 17: 11th International Vacuum Microelectronics Conference (IVMC 98) (JUL 19-23, 1998, ASHEVILLE, NORTH CAROLINA), Is. 2. - P666-669, DOI 10.1116/1.590614. - Cited References: 9 . - 4. - ISSN 1071-1023

РУБ Engineering, Electrical & Electronic + Nanoscience & Nanotechnology + Physics, Applied
Рубрики:
TIPS
   SILICON
   GROWTH
   ARRAYS
   FILMS
Аннотация: The emission of different, diamond coatings on silicon field emitter. arrays were investigated: chemical vapor deposition diamond, high pressure and high temperature synthetic diamond, and shock-synthesized nanodiamond. Practical emission characteristics such as: emission threshold, maximum current, current stability, and reproducibility were tested. The effects of surface modification, the size of diamond crystallites, and hydrogen plasma treatments were also studied. (C) 1999 American Vacuum Society. [S0734-211X(99)06102-8].

Держатели документа:
Inst Crystallog, Moscow 117333, Russia
Univ Fribourg, Dept Phys, CH-1700 Fribourg, Switzerland
Russian Fed Nucl Ctr, Snezhinsk 456770, Russia
Inst Biophys, Krasnoyarsk 660036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zhirnov, V.V.; Kuttel, O.M.; Groning, O...; Alimova, A.N.; Detkov, P.Y.; Belobrov, P.I.; Maillard-Schaller, E...; Schlapbach, L...

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Thermal properties of diamond/carbon composites [Text] / A. . Vlasov [et al.] // Diam. Relat. Mat. - 2000. - Vol. 9: 10th European Conference on Diamond, Diamond-Like Materials, Nitrides and Silicon Carbide (Diamond 1999) (SEP 12-17, 1999, PRAGUE, CZECH REPUBLIC), Is. 03.06.2013. - P. 1104-1109, DOI 10.1016/S0925-9635(99)00256-3. - Cited References: 9 . - ISSN 0925-9635

РУБ Materials Science, Multidisciplinary
Рубрики:
CVD DIAMOND
   CONDUCTIVITY
   FILMS
   RAMAN
Кл.слова (ненормированные):
diamond composites -- laser flash technique -- TEM -- thermal conductivity
Аннотация: The thermal conductivity, k, of diamond/carbon composites with different ratios of sp(2)/sp(3)-bonded carbon is measured by the laser flash technique. The thermal conductivity of nanocomposites containing 6 nm diamond particles falls within the range of k = 0.003-0.017 W/cmK; at room temperature. The thermal conductivity increases while nanopores are gradually filled with pyrolytic carbon (pyrocarbon/diamond mass ratio variation of 0.0-0.5). Transmission electron microscopy data reveal a fairly uniform mixture of two carbon phases, the diamond and matrix having similar grain sizes. Estimates show that the phonon free path is limited by dimensions of carbon matrix layer. Thermal data for coarse-grain (1-2 mu m) composites are also given for comparison. (C) 2000 Elsevier Science S.A. All rights reserved.

WOS
Держатели документа:
Inst Gen Phys, Moscow 117942, Russia
CRIM, St Petersburg, Russia
Inst Crystallog, Moscow, Russia
Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Vlasov, A...; Ralchenko, V...; Gordeev, S...; Zakharov, D...; Vlasov, I...; Karabutov, A...; Belobrov, P...

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Low-field electron emission of diamond/pyrocarbon composites / A. V. Karabutov [et al.] // Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures. - 2001. - Vol. 19: 13th International Vaccum Microelectronics Conference (14 August 2000 through 17 August 2000, Guangzhou, Is. 3. - P965-970, DOI 10.1116/1.1368669 . - ISSN 1071-1023
Кл.слова (ненормированные):
Carbon nanotubes -- Chemical bonds -- Chemical vapor deposition -- Composite materials -- Diamond films -- Electric conductivity -- Electron emission -- Electron energy levels -- Hysteresis -- Interfaces (materials) -- Raman scattering -- Semiconducting diamonds -- Semiconductor quantum wells -- Transmission electron microscopy -- X ray diffraction analysis -- X ray photoelectron spectroscopy -- Pyrocarbon composites -- Nanostructured materials
Аннотация: The properties of field electron emission for diamond/pyrocarbon nanocomposites produced from diamond particles surrounded by a pyrocarbon matrix were studied. Low-threshold emissions at fields of ?1 V/?m with no activation or hysterisis in the current versus voltage (I/V) behaviour were observed for the materials. Scanning tunneling-field emission microscopy was used to study the mechanisms of low-field electron emission from the composites, and a model based on quantum well effect at the diamond/graphite interface was proposed and discussed.

Scopus
Держатели документа:
General Physics Institute, Vavilova str. 38, Moscow 117942, Russian Federation
Central Research Institute of Materials, Paradnaya str. 8, St. Petersburg 191014, Russian Federation
Institute of Biophysics, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Karabutov, A.V.; Frolov, V.D.; Konov, V.I.; Ralchenko, V.G.; Gordeev, S.K.; Belobrov, P.I.

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10.

Low-field electron emission of diamond/pyrocarbon composites [Text] / A. V. Karabutov [et al.] // J. Vac. Sci. Technol. B. - 2001. - Vol. 19: 13th International Vacuum Microlectronics Conference (AUG 13-17, 2000, GUANGZHOU, PEOPLES R CHINA), Is. 3. - P. 965-970, DOI 10.1116/1.1368669. - Cited References: 31 . - ISSN 1071-1023

РУБ Engineering, Electrical & Electronic + Nanoscience & Nanotechnology + Physics, Applied
Рубрики:
CVD DIAMOND FILMS
   SCANNING-TUNNELING-MICROSCOPY
   AMORPHOUS-CARBON
   COLD-CATHODE
Аннотация: Properties of the field electron emission for diamond/pyrocarbon nanocomposites produced from diamond particles surrounded by an sp(2)-bonded pyrocarbon matrix are considered as functions of a size of diamond particles selected in the range of 5 nm - 5 mum, and of an average thickness of the pyrocarbon shell controlled by the pyrocarbon/diamond mass ratio varied from 0 to 0.5. The low-threshold emission at fields of greater than or equal to1 V/mum with ''no activation/no hysteresis'' I-V behavior was observed for these materials using tungsten tip microprobes as well as a fluorescent screen. A specially designed scanning tunneling-field emission microscope was used for simultaneous mapping of field emission intensity, topography, work function, and electrical resistivity to study the mechanisms of the emission from the composites and well-emitting chemical vapor deposition diamond films. It was found that for both of the materials emission centers are associated with interfaces between diamond and sp2-bonded carbon phases. Possible mechanisms of the low-field electron emission for the diamond/graphite composites including local field enhancement are analyzed. A model of the low-field emission based on quantum well effect at the diamond/graphite interface is proposed and discussed. (C) 2001 American Vacuum Society.

WOS
Держатели документа:
Russian Acad Sci, Inst Gen Phys, Moscow 117942, Russia
Cent Res Inst Mat, St Petersburg, Russia
Russian Acad Sci, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Karabutov, A.V.; Frolov, V.D.; Konov, V.I.; Ralchenko, V.G.; Gordeev, S.K.; Belobrov, P.I.

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11.

Application of satellite data for investigation of dynamic processes in inland water bodies: Lake Shira (Khakasia, Siberia), a case study / A. P. Shevyrnogov, A. V. Kartushinsky, G. S. Vysotskaya // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P153-163, DOI 10.1023/A:1015658927683 . - ISSN 1386-2588
Кл.слова (ненормированные):
Modelling -- Phytopigments -- Satellite data -- Satellite equipment -- Software -- Temperature -- AVHRR -- hydrodynamics -- lake -- limnology -- remote sensing -- saline lake -- satellite data -- water temperature -- Russian Federation
Аннотация: This work describes avenues to use satellite information to analyse dynamic processes in aquatic ecosystems. Information for this analysis, was retrieved from AVHRR satellite sensor data. This information consisteds of time series of images of radiation temperature and turbidity. We expect this information will be of great value in analysing inland water bodies. Methods to process satellite information using original software and data processing techniques are proposed. For the investigation of the process and analyses of satellite information Shira Lake (Khakasia, Siberia) was used as a case study. To study the variability of the surface temperature and turbidity of the Lake in summer, the satellite and ground-truth data of the lake was applied. This study represents the first evaluation of the dynamic processes for Lake Shira based on satellite, ground-truth and modelling data. We developed algorithms and software to process satellite images to enable the reconstruction of time dependence of temperature and spectral reflectance of water bodies in the visible range, and to make computer-animated films visualising the spatial and temporal dynamics of the study parameters. The analyses of morphometric, meteorological and hydrological characteristics of Lake Shira have provided a realistic opportunity for processing the satellite information and to develop numerical models of variability of the hydrological regime of the lake. The results obtained demonstrate the feasibility of systematically retrieving the spatial information from the satellite data on the dynamics of the surface water temperature and of the suspended matter in the lake.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shevyrnogov, A.P.; Kartushinsky, A.V.; Vysotskaya, G.S.

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12.

Development and morphological features of biofilms formed by transgenic and wild type strains of Bacillus subtilis [Text] / O. A. Mogilnaya, T. Y. Krylova, L. Y. Popova // Microbiol. Res. - 2003. - Vol. 158, Is. 4. - P. 327-335, DOI 10.1078/0944-5013-00212. - Cited References: 17 . - ISSN 0944-5013

РУБ Microbiology
Рубрики:
ANTIBIOTIC-RESISTANCE
MICROBIAL BIOFILMS
COMMUNITY
Кл.слова (ненормированные):
Bacillus subtilis -- biofilms -- transgenic microorganisms -- electron microscopy
Аннотация: The study addressed the ability of the transgenic strain (TM) B. subtilis 2335/pBMB 105 (Km(r)Inf(+)) to form biofilms on the surface of liquid media of various compositions, inoculated with vegetative cells and spores. The morphological features of these biofilms do not differ from those of the films formed by the recipient strain (WT) B. subtilis 2335 (Km(s)). However, the TM and the natural one differ in the dynamics of biofilm formation and the cellular composition of the films. Biofilms of the TM are formed earlier, develop at a higher rate, but decompose later than the films of the WT. When the medium is inoculated with vegetative cells, sporulation in the biofilms of both strains undergoes glucose repression; no such effect is observed when the medium is inoculated with spores. The TM does not form films when the medium is inoculated with spores and supplemented with glycerin and kanamycin.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Mogilnaya, O.A.; Krylova, T.Y.; Popova, L.Y.

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13.

Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood [Text] / V. I. Sevastianov [et al.] // J. Biomater. Sci.-Polym. Ed. - 2003. - Vol. 14, Is. 10. - P. 1029-1042, DOI 10.1163/156856203769231547. - Cited References: 34 . - ISSN 0920-5063

РУБ Engineering, Biomedical + Materials Science, Biomaterials + Polymer Science
Рубрики:
BIODEGRADABLE POLYESTERS
POLYMERS
Кл.слова (ненормированные):
polyhydroxyalkanoates (PHAs) -- polyhydroxybutyrate (PHB) -- poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) -- endotoxins -- beta-hydroxy acids -- hemocompatibility
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C-16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only beta-OH-C-14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Russian Minist Hlth, Inst Transplantol Artificial Organs, Moscow 123182, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sevastianov, V.I.; Perova, N.V.; Shishatskaya, E.I.; Kalacheva, G.S.; Volova, T.G.

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14.

Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood / V. I. Sevastianov [et al.] // Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - P1029-1042, DOI 10.1163/156856203769231547 . - ISSN 0920-5063
Кл.слова (ненормированные):
?-hydroxy acids -- Endotoxins -- Hemocompatibility -- Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) -- Polyhydroxyalkanoates (PHAs) -- Polyhydroxybutyrate (PHB) -- bacterium lipopolysaccharide -- carbon -- complement -- copolymer -- hydroxyacid -- long chain fatty acid -- poly(3 hydroxybutyric acid) -- polyhydroxyalkanoic acid -- valeric acid derivative -- adult -- article -- biofilm -- biotechnology -- blood analysis -- blood clotting -- blood compatibility -- cell function -- chemical analysis -- chemical composition -- complement activation -- concentration (parameters) -- controlled study -- gas chromatography -- hemostasis -- human -- human cell -- mass spectrometry -- micromorphology -- nonhuman -- priority journal -- purification -- quantitative analysis -- sampling -- synthesis -- thrombocyte adhesion -- Wautersia eutropha -- Biocompatible Materials -- Blood -- Blood Coagulation Tests -- Chromatography, Gas -- Complement Activation -- Cupriavidus necator -- Fatty Acids -- Humans -- Platelet Adhesiveness -- Polyesters -- Surface Properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.

Scopus
Держатели документа:
Inst. of Transplantol. Artif. Organs, Russian Ministry of Health, Shchukinskaya 1, 123182 Moscow, Russian Federation
Inst. of Biophys. of Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sevastianov, V.I.; Perova, N.V.; Shishatskaya, E.I.; Kalacheva, G.S.; Volova, T.G.

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15.

A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures [Text] / E. I. Shishatskaya, T. G. Volova // J. Mater. Sci.-Mater. Med. - 2004. - Vol. 15, Is. 8. - P. 915-923, DOI 10.1023/B:JMSM.0000036280.98763.c1. - Cited References: 34 . - ISSN 0957-4530

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
DEGRADATION
   POLY(3-HYDROXYBUTYRATE)
   POLYESTERS
   POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 60036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.

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16.

A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures [Text] / E. I. Shishatskaya, T. G. Volova // J. Mater. Sci.-Mater. Med. - 2004. - Vol. 15, Is. 8. - P915-923, DOI 10.1023/B:JMSM.0000036280.98763.c1. - Cited References: 34 . - 9. - ISSN 0957-4530

РУБ Engineering, Biomedical + Materials Science, Biomaterials
Рубрики:
DEGRADATION
   POLY(3-HYDROXYBUTYRATE)
   POLYESTERS
   POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.

Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 60036, Russia : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.; Volova, T.G.

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17.

Biofilm formation by bacterial associations under various salinities and copper ion stress [Text] / O. A. Mogilnaya [et al.] // Biofouling. - 2005. - Vol. 21, Is. 05.06.2013. - P. 247-255, DOI 10.1080/08924010500445848. - Cited References: 24 . - ISSN 0892-7014

РУБ Biotechnology & Applied Microbiology + Marine & Freshwater Biology
Рубрики:
HEAVY-METAL RESISTANCE
   BACILLUS-SUBTILIS
   PROTEIN
   RISK
Кл.слова (ненормированные):
binary community -- surface films -- adhesion -- copper -- stress
Аннотация: The study addresses the effect of abiotic (medium salinity and copper ions) and biotic (interactions between populations) factors on the formation of structured communities by binary associations consisting of halotolerant bacteria (Alcaligenes sp. 1-1 or Acinetobacter sp. 1-19) and a wild-type B. subtilis 2335 strain or a transgenic strain. The results showed that 250 mg l(-1) of copper ions inhibit formation of biofilms by monocultures of the tested strains. Binary associations of the strains were more resistant to high concentrations (250 mg l(-1)) of copper ions. At the lowest NaCl concentration (0.05% and 2.5%) and in the presence of copper ions, bacilli seemed to help halotolerant bacteria survive. Under increased salinity and in the presence of copper ions, structured communities developed due to halotolerant bacteria. Coexistence under stressful conditions was beneficial for the both groups of bacteria.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
TORINS Co, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Mogilnaya, O.A.; Lobova, T.I.; Kargatova, T.V.; Popova, L.Y.

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18.

Biomedical investigations of biodegradable PHAs / E. I. Shishatskaya // Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - P65-81, DOI 10.1002/masy.200850909 . - ISSN 1022-1360
Кл.слова (ненормированные):
Biodegradable polymers -- Biomedical investigations -- PHA -- ABS resins -- Biodegradable polymers -- Biopolymers -- Biotechnology -- Bone -- Cell culture -- Endothelial cells -- Fibers -- Functional polymers -- Osteoblasts -- Polymers -- Surgery -- Abdominal surgeries -- Biocompatible -- Biodegradable -- Biomedical -- Biomedical investigations -- Bone defects -- Ectopic bones -- Hepatocytes -- In vitro -- Microparticles -- Oral surgeries -- Osteogenesis -- PHA -- Polyhydroxybutyrate -- Polymer devices -- Ralstonia -- Russian academy of sciences -- Two types -- Polymer films
Аннотация: This work is a review of the results of biomedical studies of polymer devices (films, fibers, microparticles, 30 implants) made from resorbable PHAs synthesized by the bacterium Wautersia (Ralstonia) eutropha 65786, using the technology developed at the Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences. Two types of PHAs - polyhydroxybutyrate (PHB) and a hydroxybutyrate/hydroxyvalerate copolymer (PHB/PHV) - have been proven to be biocompatible in vitro in cultures of fibroblasts, endothelial cells, hepatocytes, and osteoblasts, and in short- and long-duration experiments on animals. Polymer films and membranes have been found to be usable as scaffolds for functioning cells and monofilament suture fibers - for stitching muscular-fascial wounds and in abdominal surgery. Ectopic bone formation assay and experiments with the model of segmental osteotomy showed that 30 PHB and PHB/HA implants can be used for reparative osteogenesis. The paper reports beneficial results of using polymers to repair bone defects in oral surgery. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 50, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodnui Av., 69, Krasnoyarsk, 660148, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Shishatskaya, E.I.

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19.

Biodegradation of polyhydroxyalkanoates (PHAs) in tropical coastal waters and identification of PHA-degrading bacteria / T. G. Volova [et al.] // Polymer Degradation and Stability. - 2010. - Vol. 95, Is. 12. - P2350-2359, DOI 10.1016/j.polymdegradstab.2010.08.023 . - ISSN 0141-3910
Кл.слова (ненормированные):
Biopolymers in marine environment -- Degradation of polymers -- PHA properties -- PHA-degrading microorganisms -- Polyhydroxyalkanoates, PHA -- 16S rRNA -- 3-hydroxybutyric acid -- Amorphous phase -- Bacillus sp -- Biodegradation rate -- Chemical compositions -- Coastal waters -- Degradation rate -- Degrading bacteria -- Degree of crystallinity -- Enterobacter -- Hydroxyvalerate -- Marine environment -- PHA properties -- PHA-degrading microorganisms -- Polydispersity indices -- Polyhydroxyalkanoates -- Polymer chains -- Preparation technique -- Sole carbon source -- South China Sea -- Viet Nam -- Acids -- Bacteriology -- Biodegradable polymers -- Biodegradation -- Biomolecules -- Biopolymers -- Microorganisms -- Organic polymers -- Polydispersity -- Polymer films -- Polymers -- RNA -- Seawater -- Degradation
Аннотация: Biodegradability patterns of two PHAs: a polymer of 3-hydroxybutyric acid (3-PHB) and a copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids (3-PHB/3-PHV) containing 11 mol% of hydroxyvalerate, were studied in the tropical marine environment, in the South China Sea (Nha Trang, Vietnam). No significant differences have been observed between degradation rates of 3-PHB and 3-PHB/3-PHV specimens; it has been found that under study conditions, biodegradation is rather influenced by the shape of the polymer item and the preparation technique than by the chemical composition of the polymer. Biodegradation rates of polymer films in seawater have been found to be higher than those of compacted pellets. As 3-PHB and 3-PHB/3-PHV are degraded and the specimens lose their mass, molecular weight of both polymers is decreased, i.e. polymer chains get destroyed. The polydispersity index of the PHAs grows significantly. However, the degree of crystallinity of both PHAs remains unchanged, i.e. the amorphous phase and the crystalline one are equally disintegrated. PHA-degrading microorganisms were isolated using the clear-zone technique, by inoculating the isolates onto mineral agar that contained PHA as sole carbon source. Based on the 16S rRNA analysis, the PHA-degrading strains were identified as Enterobacter sp. (four strains), Bacillus sp. and Gracilibacillus sp.В© 2010 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
Siberian Federal University, 79 Svobodny av., Krasnoyarsk 660041, Russian Federation
L.V. Kirensky Institute of Physics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russian Federation
A.N. Severtsov Institute of Ecology and Evolution RAS, 33 Leninskij Prospect, Moscow 119071, Russian Federation
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 8 Lavrentiev Ave., Novosibirsk 630090, Russian Federation
Joint Russian-Vietnam Tropical Research and Test Centre, Coastal Branch, 30 Nguyen Thien Thuat, Nha Trang, Viet Nam : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Volova, T.G.; Boyandin, A.N.; Vasiliev, A.D.; Karpov, V.A.; Prudnikova, S.V.; Mishukova, O.V.; Boyarskikh, U.A.; Filipenko, M.L.; Rudnev, V.P.; Ba Xuan, B.; Vit Dung, V.; Gitelson, I.I.

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20.

Surface modification of bioresorbable polymer scaffolds by laser treatment / V. V. Slabko [et al.] // Biophysics. - 2010. - Vol. 55, Is. 2. - P234-238, DOI 10.1134/S0006350910020120 . - ISSN 0006-3509
Кл.слова (ненормированные):
hydrophilicity -- laser irradiation -- microbial biocompatible and biodegradable polymers -- polyhydroxybuturate -- surface properties
Аннотация: The effect of laser irradiation on the properties of the surface of films prepared from a bioresorbable polymer poly(hydroxybuturate) has been studied. To determine the spectral region of the polymer optimal for the effective action of radiation on electron molecular bonds, theoretical investigations have been performed, which have shown that, for modifying the surface of PHB scaffolds, it is expedient to use a vacuum laser at a wavelength of 160 nm. Using laser irradiation at a power from 3 to 30 W, a series of films with modified surface, from roughnesses to perforations, have been obtained. The microstructure and properties of the film surface depending on the mode of irradiation have been examined, and conditions have been found under which the contact marginal angles of film wetting with water can be decreased to 50В° (compared with 76-80В° in starting products). Thus, conditions of laser treatment of PHB scaffolds have been theoretically substantiated and experimentally realized that provide a beneficial effect on the properties of the surface without destroying the structure of the material. В© 2010 Pleiades Publishing, Ltd.

Scopus
Держатели документа:
Siberian Federal University, Krasnoyarsk, Russian Federation
Institute of Biophysics, Russian Academy of Sciences, Siberian Division, Krasnoyarsk 660036, Russian Federation
Siberian State Technological University, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Slabko, V.V.; Volova, T.G.; Krasnov, P.O.; Kuzubov, A.A.; Shishatskaya, E.I.

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