Библиотека института биофизики СО РАН

Главная

Базы данных

Труды сотрудников ИБФ СО РАН - результаты поиска

Вид поиска

Каталог книг и продолжающихся изданий библиотеки Института биофизики СО РАН

Иностранные журналы библиотеки Института биофизики СО РАН

Отечественные журналы библиотеки Института биофизики СО РАН

Каталог диссертаций ИБФ СО РАН

Труды сотрудников ИБФ СО РАН

Область поиска

в найденном

Формат представления найденных документов:
полный	информационный	краткий

Отсортировать найденные документы по:
автору	заглавию	году издания	типу документа

Поисковый запрос: (<.>K=Films<.>)

Общее количество найденных документов : 72
Показаны документы с 1 по 20

1-20 21-40 41-60 61-72

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Prudnikova S.V., Karpov V.A., Ivonin V.N., D? N.L., Nguy?n T.H., Le T.M.H., Filichev N.L., Levin A.L., Filipenko M.L., Volova T.G., Gitelson I.I.
Заглавие : Microbial degradation of polyhydroxyalkanoates in tropical soils
Место публикации : International Biodeterioration and Biodegradation. - 2013. - Vol. 83. - С. 77-84. - ISSN 09648305 (ISSN) , DOI 10.1016/j.ibiod.2013.04.014
Ключевые слова (''Своб.индексиров.''): biopolymer properties--biopolymers--pha degrading microorganisms--polyhydroxyalkanoates (phas)--soil biodegradation--3-hydroxybutyric acid--degree of crystallinity--hydroxyalkanoic acids--microbial characteristics--microbial degradation--polyhydroxyalkanoates--polymer molecular mass--soil biodegradations--bacteriology--biomolecules--biopolymers--microorganisms--soils--tropics--biodegradation--biodegradation--chemical composition--microbial activity--microbial community--molecular analysis--organic compound--polymer--soil degradation--soil microorganism--tropical soil
Аннотация: The integrated study addressing biodegradation of microbial linear polyesters of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) in tropical conditions by microbial communities of Vietnamese soils was performed in locations close to Hanoi and Nha Trang, which differed in their weather conditions and microbial communities. It shows that PHA degradation in tropical soils is influenced by polymer chemical composition, specimen shape, and microbial characteristics. The homopolymer of 3-hydroxybutyric acid is degraded at higher rates than the copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids. The average rates of mass loss were 0.04-0.33% per day for films and 0.02-0.18% for compact pellets. PHA degradation was accompanied by a decrease in the polymer molecular mass and, usually, an increase in the degree of crystallinity, suggesting preferential degradation of the amorphous phase. Under the study conditions, representatives of the bacterial genera Burkholderia, Bacillus, Cupriavidus, Mycobacterium, and Nocardiopsis and such micromycetes as Acremonium, Gongronella, Paecilomyces, and Penicillium, Trichoderma have been identified as major PHA degraders. В© 2013 Elsevier Ltd.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Rudnev V.P., Ivonin V.N., Prudnikova S.V., Korobikhina K.I., Filipenko M.L., Volova T.G., Sinskey A.J.
Заглавие : Biodegradation of polyhydroxyalkanoate films in natural environments
Место публикации : Macromolecular Symposia. - 2012. - Vol. 320, Is. 1. - С. 38-42. - ISSN 10221360 (ISSN) , DOI 10.1002/masy.201251004
Ключевые слова (''Своб.индексиров.''): biodegradation--biopolymers--microbial degradation--polyhydroxyalkanoates--acinetobacters--acremonium--alcaligenes--burkholderia--degradation rate--degrading activities--degrading bacteria--enterobacter--hydroxyvalerate--mass loss--microbial degradation--micromycetes--natural environments--paecilomyces--poly-3-hydroxybutyrate--poly-hydroxyalkanoate--polyhydroxyalkanoates--siberia--south china sea--stenotrophomonas--trichoderma--tropical soils--viet nam--xanthomonas--bacteria--bacteriology--biopolymers--degradation--seawater--soils--tropics--biodegradation
Аннотация: Biodegradation of film specimens from polyhydroxyalkanoates (PHAs) of two types - poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-3- hydroxyvalerate (PHBV) - was analysed in different environments: tropical sea waters of the South China Sea (Nha Trang, Vietnam) and soils in the environs of Hanoi (Vietnam), Nha Trang (Vietnam) and Krasnoyarsk (Siberia, Russia). In seawater, the mass loss of the specimens of both types was almost equal. However, in tropical soils, PHB degraded quicker than PHBV. In the Siberian soil, the degradation rate of the PHBV was generally higher than that of PHBV. Analysis of molecular mass of PHA specimens showed its decreasing during biodegradation. In the tropical sea conditions, PHA degrading microorganisms were represented by bacteria of Enterobacter, Bacillus and Gracilibacillus genera. Among PHA degrading bacteria, Burkholderia, Alcaligenes, Bacillus, Mycobacterium and Streptomyces genera were identified in Vietnamese soils, and Variovorax, Stenotrophomonas, Acinetobacter, Pseudomonas, Bacillus and Xanthomonas genera in Siberian soils. Micromycetes of Gongronella, Paecilomyces, Penicillium and Trichoderma genera exhibited PHA degrading activity in Vietnamese soils, and Paecilomyces, Penicillium, Acremonium, Verticillium and Zygosporium genera - in Siberian soils. Copyright В© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Boyandin A.N., Vasil'ev A.D., Karpov V.A., Kozhevnikov I.V., Prudnikova S.V., Rudnev V.P., Xuan B.B., Dung V.V., Gitel'zon I.I.
Заглавие : Biodegradation of polyhydroxyalkanoates (PHAs) in the South China Sea and identification of PHA-degrading bacteria
Место публикации : Microbiology. - 2011. - Vol. 80, Is. 2. - С. 252-260. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261711020184
Ключевые слова (''Своб.индексиров.''): biodegradation in marine environments--microbial pha degraders--pha--polyhydroxyalkanoates--bacillus (bacterium)--bacillus sp.--bacteria (microorganisms)--enterobacter--enterobacter cloacae--gracilibacillus--prospect hill virus
Аннотация: The biodegradation patterns of two types of PHA, a 3-hydroxybutyrate (3-PHB) polymer and a 3-hydroxybutyrate and 3-hydroxyvalerate (3-PHB/3-PHV) copolymer, were studied in tropical marine environments (Dam Bay, South China Sea, Nha Trang, Vietnam). No reliable differences in the degradation of 3-PHB and 3-PHB/3-PHV were revealed. It was shown that the degradation process depended mainly on the shape of a polymer product and its production method: the degradation of polymer films was found to be more active than that of molded solids. A decrease in the molecular mass of both types of PHA was detected in the course of the degradation of PHA samples. However, the degree of PHA crystallinity did not change; that is, the levels of degradation of both the amorphous and crystalline phases of PHA were almost the same. Among microbial PHA degraders, three bacterial strains, Bacillus sp. IBP-V002, Enterobacter cloacae sp. IBP-V001, and Gracilibacillus sp. IBP-V003, were identified based on the results of morphological, biochemical, and molecular phylogenetic analyses. The ability of the representatives of the genera Gracilibacillus and Enterobacter to degrade PHA was revealed for the first time. В© 2011 Pleiades Publishing, Ltd.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Boyandin A.N., Vasiliev A.D., Karpov V.A., Prudnikova S.V., Mishukova O.V., Boyarskikh U.A., Filipenko M.L., Rudnev V.P., Ba Xuan B., Vit Dung V., Gitelson I.I.
Заглавие : Biodegradation of polyhydroxyalkanoates (PHAs) in tropical coastal waters and identification of PHA-degrading bacteria
Место публикации : Polymer Degradation and Stability. - 2010. - Vol. 95, Is. 12. - С. 2350-2359. - ISSN 01413910 (ISSN) , DOI 10.1016/j.polymdegradstab.2010.08.023
Ключевые слова (''Своб.индексиров.''): biopolymers in marine environment--degradation of polymers--pha properties--pha-degrading microorganisms--polyhydroxyalkanoates, pha--16s rrna--3-hydroxybutyric acid--amorphous phase--bacillus sp--biodegradation rate--chemical compositions--coastal waters--degradation rate--degrading bacteria--degree of crystallinity--enterobacter--hydroxyvalerate--marine environment--pha properties--pha-degrading microorganisms--polydispersity indices--polyhydroxyalkanoates--polymer chains--preparation technique--sole carbon source--south china sea--viet nam--acids--bacteriology--biodegradable polymers--biodegradation--biomolecules--biopolymers--microorganisms--organic polymers--polydispersity--polymer films--polymers--rna--seawater--degradation
Аннотация: Biodegradability patterns of two PHAs: a polymer of 3-hydroxybutyric acid (3-PHB) and a copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids (3-PHB/3-PHV) containing 11 mol% of hydroxyvalerate, were studied in the tropical marine environment, in the South China Sea (Nha Trang, Vietnam). No significant differences have been observed between degradation rates of 3-PHB and 3-PHB/3-PHV specimens; it has been found that under study conditions, biodegradation is rather influenced by the shape of the polymer item and the preparation technique than by the chemical composition of the polymer. Biodegradation rates of polymer films in seawater have been found to be higher than those of compacted pellets. As 3-PHB and 3-PHB/3-PHV are degraded and the specimens lose their mass, molecular weight of both polymers is decreased, i.e. polymer chains get destroyed. The polydispersity index of the PHAs grows significantly. However, the degree of crystallinity of both PHAs remains unchanged, i.e. the amorphous phase and the crystalline one are equally disintegrated. PHA-degrading microorganisms were isolated using the clear-zone technique, by inoculating the isolates onto mineral agar that contained PHA as sole carbon source. Based on the 16S rRNA analysis, the PHA-degrading strains were identified as Enterobacter sp. (four strains), Bacillus sp. and Gracilibacillus sp.В© 2010 Elsevier Ltd. All rights reserved.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Slabko V.V., Volova T.G., Krasnov P.O., Kuzubov A.A., Shishatskaya E.I.
Заглавие : Surface modification of bioresorbable polymer scaffolds by laser treatment
Место публикации : Biophysics. - 2010. - Vol. 55, Is. 2. - С. 234-238. - ISSN 00063509 (ISSN) , DOI 10.1134/S0006350910020120
Ключевые слова (''Своб.индексиров.''): hydrophilicity--laser irradiation--microbial biocompatible and biodegradable polymers--polyhydroxybuturate--surface properties
Аннотация: The effect of laser irradiation on the properties of the surface of films prepared from a bioresorbable polymer poly(hydroxybuturate) has been studied. To determine the spectral region of the polymer optimal for the effective action of radiation on electron molecular bonds, theoretical investigations have been performed, which have shown that, for modifying the surface of PHB scaffolds, it is expedient to use a vacuum laser at a wavelength of 160 nm. Using laser irradiation at a power from 3 to 30 W, a series of films with modified surface, from roughnesses to perforations, have been obtained. The microstructure and properties of the film surface depending on the mode of irradiation have been examined, and conditions have been found under which the contact marginal angles of film wetting with water can be decreased to 50В° (compared with 76-80В° in starting products). Thus, conditions of laser treatment of PHB scaffolds have been theoretically substantiated and experimentally realized that provide a beneficial effect on the properties of the surface without destroying the structure of the material. В© 2010 Pleiades Publishing, Ltd.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sevastianov V.I., Perova N.V., Shishatskaya E.I., Kalacheva G.S., Volova T.G.
Заглавие : Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2003. - Vol. 14, Is. 10. - С. 1029-1042. - ISSN 09205063 (ISSN) , DOI 10.1163/156856203769231547
Ключевые слова (''Своб.индексиров.''): ?-hydroxy acids--endotoxins--hemocompatibility--poly(hydroxybutyrate-co-hydroxyvalerate) (phbv)--polyhydroxyalkanoates (phas)--polyhydroxybutyrate (phb)--bacterium lipopolysaccharide--carbon--complement--copolymer--hydroxyacid--long chain fatty acid--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--valeric acid derivative--adult--article--biofilm--biotechnology--blood analysis--blood clotting--blood compatibility--cell function--chemical analysis--chemical composition--complement activation--concentration (parameters)--controlled study--gas chromatography--hemostasis--human--human cell--mass spectrometry--micromorphology--nonhuman--priority journal--purification--quantitative analysis--sampling--synthesis--thrombocyte adhesion--wautersia eutropha--biocompatible materials--blood--blood coagulation tests--chromatography, gas--complement activation--cupriavidus necator--fatty acids--humans--platelet adhesiveness--polyesters--surface properties
Аннотация: Samples of olyhydroxyalkanoates (PHAs), polyhydroxybutyrate (PHB) and copolymers poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) with 4 and 18 mol% hydroxyvalerate, synthesized by the bacteria Ralstonia eutropha B5786, were investigated. PHA films in contact with blood did not activate the hemostasis system at the level of cell response, but they did activate the coagulation system and the complement reaction. To detect biologically-active components in the PHAs, a detailed analysis of the composition of the polymers was conducted. Gas chromatography-mass spectrometry revealed long-chain fatty acids (FAs) in the tested PHAs. Their total concentration in the polymer ranged from tenths of mol% to 2-3 mol%, depending on the purification method. C16:0 constituted the largest proportion, up to 70%. Of the long-chain hydroxy acids, only ?-OH-C14:0 was detected and it did not exceed 0.06 mol%. The analysis of the hemocompatibility properties of the PHAs purified by a specialized procedure, including the quantitative and morphological estimation of platelets adherent to the surface of polymer films, the plasma recalcification time and complement activation studies, indicated that PHB and PHBV can be used in contact with blood. It has been found out that the lipopolysaccharides of bacteria producing PHAs, which contain mostly long-chain hydroxy acids, can be the factor activating the hemostasis systems. Thus, the technology of PHA purification must satisfy rather stringent specific requirements.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Karabutov A.V., Frolov V.D., Konov V.I., Ralchenko V.G., Gordeev S.K., Belobrov P.I.
Заглавие : Low-field electron emission of diamond/pyrocarbon composites
Место публикации : Journal of Vacuum Science and Technology B: Microelectronics and Nanometer Structures. - 2001. - Vol. 19: 13th International Vaccum Microelectronics Conference (14 August 2000 through 17 August 2000, Guangzhou, Is. 3. - С. 965-970. - ISSN 10711023 (ISSN) , DOI 10.1116/1.1368669
Ключевые слова (''Своб.индексиров.''): carbon nanotubes--chemical bonds--chemical vapor deposition--composite materials--diamond films--electric conductivity--electron emission--electron energy levels--hysteresis--interfaces (materials)--raman scattering--semiconducting diamonds--semiconductor quantum wells--transmission electron microscopy--x ray diffraction analysis--x ray photoelectron spectroscopy--pyrocarbon composites--nanostructured materials
Аннотация: The properties of field electron emission for diamond/pyrocarbon nanocomposites produced from diamond particles surrounded by a pyrocarbon matrix were studied. Low-threshold emissions at fields of ?1 V/?m with no activation or hysterisis in the current versus voltage (I/V) behaviour were observed for the materials. Scanning tunneling-field emission microscopy was used to study the mechanisms of low-field electron emission from the composites, and a model based on quantum well effect at the diamond/graphite interface was proposed and discussed.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Maillard-Schaller E., Kuettel O.M., Diederich L., Schlapbach L., Zhirnov V.V., Belobrov P.I.
Заглавие : Surface properties of nanodiamond films deposited by electrophoresis on Si(100)
Место публикации : Diamond and Related Materials. - 1999. - Vol. 8, Is. 2-5. - С. 805-808. - ISSN 09259635 (ISSN)
Ключевые слова (''Своб.индексиров.''): energy band diagram--nanodiamond--raman spectroscopy--surface characterization--band structure--electrodeposition--electrophoresis--hydrogen--nanostructured materials--nitrogen--oxidation--oxygen--phonons--plasma applications--silicon wafers--surface properties--dielectrophoresis--negative electron affinity (nea)--phonon confinement effect--diamond films
Аннотация: The surface properties of diamond nanoparticles (40-50 A in diameter) have been investigated by X-ray photoelectron spectroscopy (XPS), UV photoelectron spectroscopy (UPS) and Raman spectroscopy. The diamond nanoparticles have been deposited on flat Si(100) substrates by electrophoresis/dielectrophoresis. The as-deposited films are strongly oxidized and present a 1-2% nitrogen content. After treatment at 850 В°C in H2 plasma for 60 min, the oxygen is removed, and the position of the C 1s core-level peak indicates a n-type electronic comportment of the diamond nanoparticles. Raman spectroscopy of the as-deposited film shows a sp3 contribution at 1321 cm-1 and a sp2 contribution around 1620 cm-1. The 12 cm-1 shift of the sp3 contribution with respect to the bulk diamond peak at 1333 cm-1 is attributed to a phonon confinement effect due to the size of the diamond particles. The H2 plasma treatment induces a size decrease of the nanocrystallites confirmed by Raman and scanning electron microscopy (SEM) measurements. UPS spectroscopy shows a negative electron affinity of -0.2 eV of the hydrogenated nanodiamond film.
Scopus
Найти похожие

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shevyrnogov A.P., Kartushinsky A.V., Vysotskaya G.S.
Заглавие : Application of satellite data for investigation of dynamic processes in inland water bodies: Lake Shira (Khakasia, Siberia), a case study
Место публикации : Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - С. 153-163. - ISSN 13862588 (ISSN) , DOI 10.1023/A:1015658927683
Ключевые слова (''Своб.индексиров.''): modelling--phytopigments--satellite data--satellite equipment--software--temperature--avhrr--hydrodynamics--lake--limnology--remote sensing--saline lake--satellite data--water temperature--russian federation
Аннотация: This work describes avenues to use satellite information to analyse dynamic processes in aquatic ecosystems. Information for this analysis, was retrieved from AVHRR satellite sensor data. This information consisteds of time series of images of radiation temperature and turbidity. We expect this information will be of great value in analysing inland water bodies. Methods to process satellite information using original software and data processing techniques are proposed. For the investigation of the process and analyses of satellite information Shira Lake (Khakasia, Siberia) was used as a case study. To study the variability of the surface temperature and turbidity of the Lake in summer, the satellite and ground-truth data of the lake was applied. This study represents the first evaluation of the dynamic processes for Lake Shira based on satellite, ground-truth and modelling data. We developed algorithms and software to process satellite images to enable the reconstruction of time dependence of temperature and spectral reflectance of water bodies in the visible range, and to make computer-animated films visualising the spatial and temporal dynamics of the study parameters. The analyses of morphometric, meteorological and hydrological characteristics of Lake Shira have provided a realistic opportunity for processing the satellite information and to develop numerical models of variability of the hydrological regime of the lake. The results obtained demonstrate the feasibility of systematically retrieving the spatial information from the satellite data on the dynamics of the surface water temperature and of the suspended matter in the lake.
Scopus
Найти похожие

10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Levin L.A., Sushchik N.N.
Заглавие : The influence of a monolayer of an aliphatic acid on temperature oscillation in the surface layer of water
Место публикации : Izvestiya - Atmospheric and Ocean Physics. - 1996. - Vol. 32, Is. 3. - С. 378-380. - ISSN 00014338 (ISSN)
Ключевые слова (''Своб.индексиров.''): aliphatic acid--surface films--temperature--thermal spots
Аннотация: The influence of a monolayer of a palmitic acid on amplitude-frequency characteristics of temperature oscillations caused by thermal spots of a surface film was experimentally determined in a Langmuir tank. An analysis of the power spectra showed that the temperature oscillations were multimodal. In addition, no consistant differences between the spectrum for a pure surface and the spectrum for a surface covered by an acid monolayer were found. A roughened gain-frequency factor was found that is equal to the ratio of the average period between the thermal spots to the dispersion of the time series of temperature oscillations. The factor significantly differed over a wide range of temperatures for a pure surface and for a surface covered with the monolayer. The experimental results do not confirm the existence of a leading frequency in the temperature oscillations of a cold surface film due to an appearance of thermal spots.
Scopus
Найти похожие

11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Belyaeva O.G., Plotnikov V.F., Puzyr' A.P.
Заглавие : Studies of Biodegradation of Microbial Polyhydroxyalkanoates
Место публикации : Prikladnaya Biokhimiya i Mikrobiologiya. - 1998. - Vol. 34, Is. 5. - С. 543. - ISSN 05551099 (ISSN)
Аннотация: Biodegradation of polyhydroxyalkanoates (PHA) - polymers of hydroxylated fatty acids - differing in chemical composition, crystallinity, melting temperature, and molecular weight was studied in soil and water environments under conditions of varying ambient temperature and pH. Under similar conditions, homogeneous films of polyhydroxybutyrate (PHB) with different initial properties were degraded at virtually equal rates. Hydroxybutyrate-hydroxyvalerate copolymers with a lower crystallinity and different microstructure in comparison to PHB were degraded at a 20 to 30% higher rate on average. The ambient temperature significantly influenced the rate of PHA biodegradation.
Scopus
Найти похожие

12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Belyaeva O.G., Plotnikov V.F., Puzyr' A.P.
Заглавие : Studies of biodegradation of microbial polyhydroxyalkanoates
Место публикации : Applied Biochemistry and Microbiology. - 1998. - Vol. 34, Is. 5. - С. 488-492. - ISSN 00036838 (ISSN)
Аннотация: Biodegradation of polyhydroxyalkanoates (PHA) - polymers of hydroxylated fatty acids - differing in chemical composition, crystallinity, melting temperature, and molecular weight was studied in soil and water environments under conditions of varying ambient temperature and pH. Under similar conditions, homogeneous films of polyhydroxybutyrate (PHB) with different initial properties were degraded at virtually equal rates. Hydroxybutyrate-hydroxyvalerate copolymers with a lower crystallinity and different microstructure in comparison to PHB were degraded at a 20 to 30% higher rate on average. The ambient temperature significantly influenced the rate of PHA biodegradation.
Scopus
Найти похожие

13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I.
Заглавие : Biomedical investigations of biodegradable PHAs
Место публикации : Macromolecular Symposia. - 2008. - Vol. 269, Is. 1. - С. 65-81. - ISSN 10221360 (ISSN) , DOI 10.1002/masy.200850909
Ключевые слова (''Своб.индексиров.''): biodegradable polymers--biomedical investigations--pha--abs resins--biodegradable polymers--biopolymers--biotechnology--bone--cell culture--endothelial cells--fibers--functional polymers--osteoblasts--polymers--surgery--abdominal surgeries--biocompatible--biodegradable--biomedical--biomedical investigations--bone defects--ectopic bones--hepatocytes--in vitro--microparticles--oral surgeries--osteogenesis--pha--polyhydroxybutyrate--polymer devices--ralstonia--russian academy of sciences--two types--polymer films
Аннотация: This work is a review of the results of biomedical studies of polymer devices (films, fibers, microparticles, 30 implants) made from resorbable PHAs synthesized by the bacterium Wautersia (Ralstonia) eutropha 65786, using the technology developed at the Institute of Biophysics of the Siberian Branch of the Russian Academy of Sciences. Two types of PHAs - polyhydroxybutyrate (PHB) and a hydroxybutyrate/hydroxyvalerate copolymer (PHB/PHV) - have been proven to be biocompatible in vitro in cultures of fibroblasts, endothelial cells, hepatocytes, and osteoblasts, and in short- and long-duration experiments on animals. Polymer films and membranes have been found to be usable as scaffolds for functioning cells and monofilament suture fibers - for stitching muscular-fascial wounds and in abdominal surgery. Ectopic bone formation assay and experiments with the model of segmental osteotomy showed that 30 PHB and PHB/HA implants can be used for reparative osteogenesis. The paper reports beneficial results of using polymers to repair bone defects in oral surgery. Copyright В© 2008 WILEY-VCH Verlag GmbH & Co. KGaA.
Scopus
Найти похожие

14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Nikolaeva E.D., Goncharov D.B., Shishatskaya E.I.
Заглавие : Effects of H 2O 2-plasma processing on properties of cellular scaffolds made of В«BioplastotanВ» resorbing polyesters
Место публикации : Cellular Transplantation and Tissue Engineering. - 2011. - Vol. 6, Is. 2. - С. 65-69. - ISSN 1815445X (ISSN)
Ключевые слова (''Своб.индексиров.''): adhesion--bioplastotan--cell viability--h 2o 2-plasma processing--resorbing polyesters--scaffold
Аннотация: Produced from В«BioplastotanВ» resorbing polyesters (linear polyesters of hydroxyl derivatives alkanoic acids) scaffolds for cell culturing such as films, pressed 3-D forms and nonwoven fabric from ultrathin fibers are characterized. Two types of polymers - a homopolymer of the 3-hydroxybutyric acid and a copolymer formed by monomers of the 3-hydroxybutyric and 3-hydroxyvalerianic acids are studied. Surface properties of developed polymer scaffolds, sterilized with autoclaving and H 2O 2-plasma processing are compared. It is shown that plasma has beneficial effects resulting in decrease of the watering contact angle and increase of surface hydrophilic properties. Positive effects of H 2O 2-plasma processing of scaffold surface on culturing cell adhesion and viability compared with autoclaving sterilization is demonstrated on NIH 3T3 line fibroblast culturing.
Scopus
Найти похожие

15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A.N., Prudnikova S.V., Karpov V.A., Ivonin V.N., Do N.L., Nguyen T.H., Le TMH, Filichev N.L., Levin A.L., Filipenko M.L., Volova T.G., Gitelson I.I.
Заглавие : Microbial degradation of polyhydroxyalkanoates in tropical soils
Место публикации : Int. Biodeterior. Biodegrad.: ELSEVIER SCI LTD, 2013. - Vol. 83. - С. 77-84. - 8. - ISSN 0964-8305, DOI 10.1016/j.ibiod.2013.04.014
Примечания : Cited References: 74. - This study was financially supported by Project "Biotechnologies of novel biomaterials" (Agreement No. 11.G34.31.0013) in accordance with Resolution No. 220 of the Government of the Russian Federation of April 9, 2010, "On measures designed to attract leading scientists to the Russian institutions of higher learning".
Предметные рубрики: POLY-BETA-HYDROXYBUTYRATE
CHAIN-LENGTH POLYHYDROXYALKANOATES
POLY(3-HYDROXYBUTYRATE) DEPOLYMERASE
COASTAL WATERS
PHA FILMS
BIODEGRADATION
BACTERIA
IDENTIFICATION
POLYESTERS
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates (phas)--biopolymers--biopolymer properties--soil biodegradation--pha degrading microorganisms
Аннотация: The integrated study addressing biodegradation of microbial linear polyesters of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) in tropical conditions by microbial communities of Vietnamese soils was performed in locations close to Hanoi and Nha Trang, which differed in their weather conditions and microbial communities. It shows that PHA degradation in tropical soils is influenced by polymer chemical composition, specimen shape, and microbial characteristics. The homopolymer of 3-hydroxybutyric acid is degraded at higher rates than the copolymer of 3-hydroxybutyric and 3-hydroxyvaleric acids. The average rates of mass loss were 0.04-0.33% per day for films and 0.02-0.18% for compact pellets. PHA degradation was accompanied by a decrease in the polymer molecular mass and, usually, an increase in the degree of crystallinity, suggesting preferential degradation of the amorphous phase. Under the study conditions, representatives of the bacterial genera Burkholderia, Bacillus, Cupriavidus, Mycobacterium, and Nocardiopsis and such micromycetes as Acremonium, Gongronella, Paecilomyces, and Penicillium, Trichoderma have been identified as major PHA degraders. (C) 2013 Elsevier Ltd. All rights reserved.
Найти похожие

16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G.
Заглавие : A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures
Место публикации : J. Mater. Sci.-Mater. Med.: KLUWER ACADEMIC PUBL, 2004. - Vol. 15, Is. 8. - С. 915-923. - 9. - ISSN 0957-4530, DOI 10.1023/B:JMSM.0000036280.98763.c1
Примечания : Cited References: 34
Предметные рубрики: DEGRADATION
POLY(3-HYDROXYBUTYRATE)
POLYESTERS
POLYMERS
Аннотация: The paper describes the production and investigation of flexible films made of high-purity polyhydroxyalkanoates (PHAs) - polyhydroxybutyrate [poly-(3HB)] and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate [poly(3Hl3-co-3HV)], containing 4-30 mol % hydroxyvalerate. Poly(3HB-co-3HV) films have a more porous structure than poly-(3HB) films, which are more compact, but their surface properties, such as wettability and surface and interface energies, are the same. Sterilisation of the PHA films by conventional methods (heat treatment and gamma-irradiation) did not impair their strength. Cells cultured on PHA films exhibited high levels of cell adhesion. Cell morphology, protein synthesis and DNA synthesis were estimated by extent of H-3-thymidine incorporation into the animal cell cultures of various origins (fibroblasts, endothelium cells, and isolated hepatocytes) in direct contact with PHAs. The investigation showed that this material can be used to make matrices for in vitro proliferous cells. The investigated properties of poly-(3HB) and poly(3HB-co-3HV) films proved to be fundamentally similar. (C) 2004 Kluwer Academic Publishers.
Найти похожие

17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : VOLOVA T.G., KALACHEVA G.S., KONSTANTINOVA V.M.
Заглавие : ACCUMULATION OF POLYHYDROXYBUTYRATE BY THE AEROBIC CO-OXIDIZING BACTERIUM SELIBERIA-CARBOXYDOHYDROGENA
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1994. - Vol. 63, Is. 2. - С. 109-112. - 4. - ISSN 0026-2617
Примечания : Cited References: 20
Аннотация: The synthesis of polyhydroxybutyrate by the carboxydobacterium Seliberia carboxydohydrogena under various growth conditions was studied. The bacterium was able to accumulate poly-beta-hydroxybutyric acid and a heteropolymer that was a product of copolymerization of hydroxybutyric and hydroxyvaleric acids. Nitrogen and sulfur exerted the strongest effect on polymer accumulation in carboxydobacteria: The maximum polymer concentrations in cells reached 28%. Specimens of polyhydroxybutyrate films were obtained, and their degradability by soil microflora was shown.
Найти похожие

18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhirnov V.V., Kuttel O.M., Groning O..., Alimova A.N., Detkov P.Y., Belobrov P.I., Maillard-Schaller E..., Schlapbach L...
Заглавие : Characterization of field emission cathodes with different forms of diamond coatings
Место публикации : J. Vac. Sci. Technol. B: AMER INST PHYSICS, 1999. - Vol. 17: 11th International Vacuum Microelectronics Conference (IVMC 98) (JUL 19-23, 1998, ASHEVILLE, NORTH CAROLINA), Is. 2. - С. 666-669. - 4. - ISSN 1071-1023, DOI 10.1116/1.590614
Примечания : Cited References: 9
Предметные рубрики: TIPS
SILICON
GROWTH
ARRAYS
FILMS
Аннотация: The emission of different, diamond coatings on silicon field emitter. arrays were investigated: chemical vapor deposition diamond, high pressure and high temperature synthetic diamond, and shock-synthesized nanodiamond. Practical emission characteristics such as: emission threshold, maximum current, current stability, and reproducibility were tested. The effects of surface modification, the size of diamond crystallites, and hydrogen plasma treatments were also studied. (C) 1999 American Vacuum Society. [S0734-211X(99)06102-8].
Найти похожие

19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Prudnikova S.V., Boyandin A.N., Kalacheva G.S., Sinskey A.J.
Заглавие : Degradable Polyhydroxyalkanoates as Herbicide Carriers
Коллективы : Government of the Russian Federation [11.G34.31.0013]
Место публикации : J. Polym. Environ.: SPRINGER/PLENUM PUBLISHERS, 2013. - Vol. 21, Is. 3. - С. 675-682. - 8. - ISSN 1566-2543, DOI 10.1007/s10924-012-0561-z
Примечания : Cited References: 31. - We thank Dr. Christopher Brigham for critical review of the manuscript prior to submission. The study was supported by the project initiated by the Government of the Russian Federation for governmental support of scientific research conducted under the guidance of leading scientists at Russian institutions of higher learning (Agreement No. 11.G34.31.0013).
Предметные рубрики: CONTROLLED-RELEASE
SOIL
BIODEGRADATION
POLYESTERS
PESTICIDE
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
MICROSPHERES
BACTERIA
Ключевые слова (''Своб.индексиров.''): herbicide--haloxyfop-p-methyl--zellek super--sustained-release formulations--polyhydroxyalkanoates--plant growth suppression
Аннотация: The biodegradable polymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate) has been used to design experimental sustained-release formulations of the herbicide Zellek Super in the form of films and microgranules. The kinetics of polymer degradation and the dynamics of herbicide release show that the rate and extent of herbicide release from the polymer matrix into the soil depends on the geometry of the carrier and the proportion of the pesticide loaded into it (polymer/pesticide mass ratio). Experiments with the creeping bentgrass (Agrostis stolonifera L.) show that the formulations of the herbicide Zellek Super constructed as microgranules and films can be successfully used to suppress the growth of grasses. This study is the first to demonstrate that biodegradable polyhydroxyalkanoates can be used effectively to construct environmentally friendly sustained-release PHA-herbicide systems that can be placed into the soil together with seeds.
Найти похожие

20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Boyandin A. N., Prudnikova S.V., Filipenko M.L., Khrapov E.A., Vasiliev A. D., Volova, Tatiana G.
Заглавие : Biodegradation of polyhydroxyalkanoates by soil microbial communities of different structures and detection of PHA degrading microorganisms
Коллективы : Government of the Russian Federation [11.G34.31.0013]; Presidium of the Siberian Branch of Russian Academy of Sciences [96]
Место публикации : Appl. Biochem. Microbiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2012. - Vol. 48, Is. 1. - С. 28-36. - 9. - ISSN 0003-6838, DOI 10.1134/S0003683812010024
Примечания : Cited References: 39. - The work was supported by the project initiated by the Government of the Russian Federation for governmental support of scientific research conducted under the guidance of leading scientists at Russian institutions of higher learning (Agreement No. 11.G34.31.0013) and the Program of Integrated Research of the Presidium of the Siberian Branch of Russian Academy of Sciences (project no. 96).
Предметные рубрики: POLY-BETA-HYDROXYBUTYRATE
CHAIN-LENGTH POLYHYDROXYALKANOATES
DEGRADATION
FILMS
POLY(3-HYDROXYBUTYRATE-CO-3-HYDROXYVALERATE)
BACTERIA
ACID
Аннотация: Biodegradation of microbial linear polymers of hydroxyalkanoic acids (polyhydroxyalkanoates, PHAs) by soil microbial communities of different structures has been studied during two field seasons in different weather conditions. This process was shown to be influenced by the polymer chemical composition, temperature, humidity, and the microbial soil component. The PHA degradation was accompanied by a decrease in the polymer molecular weight and an increase in the degree of crystallinity, indicating the preferential destruction of the amorphous phase compared to the crystalline one. The quantity of the true PHA destructors developing at the surface of the polymer samples was lower than the quantity of accompanying bacteria. The dominant PHA degrading microorganisms under the test conditions were identified as bacteria of the genera Variovorax, Stenotrophomonas, Acinetobacter, Pseudomonas, Bacillus, and Xanthomonas and as micromycetes from Penicillium, Paecilomyces, Acremonium, Verticillium, and Zygosporium.
WOS,
SCOPUS,
Полный текст на сайте издателя
Найти похожие