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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Илларионов Б.А., Протопопова М.В., Киргинов В.А., Мертвецов И.И., Гительзон И.И.
Заглавие : Нуклеотидная последовательность генов 'альфа'- и 'бета'-субъединиц люциферазы Photobacterium leiognathi : научное издание
Место публикации : Биоорган. химия. - 1988. - Т. 14, N 3. - С. 412-415. - ISSN 0132-3423
ГРНТИ : 34.15.25
Предметные рубрики: ГЕН ЛЮЦИФЕРАЗЫ
СУБЪЕДИНИЦА 'АЛЬФА'
СУБЪЕДИНИЦА 'БЕТА'
НУКЛЕОТИДНАЯ ПОСЛЕДОВАТЕЛЬНОСТЬ
ЛЮЦИФЕРАЗА
АМИНОКИСЛОТНАЯ ПОСЛЕДОВАТЕЛЬНОСТЬ
ВЫВЕДЕННАЯ
PHOTOBACTERIUM LEIOGNATHI
БАКТЕРИИ
LUCIFERASE GENE
'АЛЬФА' А 'БЕТА' В Т
С ЕОТ Е Е Е СЕ
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Markova, Svetlana V., Larionova, Marina D., Burakova, Ludmila P., Vysotski, Eugene S.
Заглавие : The smallest natural high-active luciferase: Cloning and characterization of novel 16.5-kDa luciferase from copepod Metridia longa
Колич.характеристики :6 с
Коллективы : Bayer AG (Germany); Russian Science Foundation [14-14-01119]
Место публикации : Biochem. Biophys. Res. Commun.: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2015. - Vol. 457, Is. 1. - С. 77-82. - ISSN 0006-291X, DOI 10.1016/j.bbrc.2014.12.082. - ISSN 1090-2104(eISSN)
Примечания : Cited References:20. - The cloning of cDNA encoding MLuc7 luciferase of M. longa was supported by Bayer AG (Germany); all other studies - by the grant 14-14-01119 of the Russian Science Foundation. We declare that authors have no conflict of interest.
Предметные рубрики: CDNA CLONING
SECRETED LUCIFERASE
ESCHERICHIA-COLI
EXPRESSION
Ключевые слова (''Своб.индексиров.''): bioluminescence--coelenterazine--copepod luciferase--mammalian--expression--real-time imaging
Аннотация: Coelenterazine-dependent copepod luciferases containing natural signal peptide for secretion are a very convenient analytical tool as they enable monitoring of intracellular events with high sensitivity, without destroying cells or tissues. This property is well suited for application in biomedical research and development of cell-based assays for high throughput screening. We report the cloning of cDNA gene encoding a novel secreted non-allelic 16.5-kDa isoform (MLuc7) of Metridia longa luciferase, which, in fact, is the smallest natural luciferase of known for today. Despite the small size, isoform contains 10 conservative Cys residues suggesting the presence of up to 5 S-S bonds. This hampers the efficient production of functionally active recombinant luciferase in bacterial expression systems. With the use of the baculovirus expression system, we produced substantial amounts of the proper folded MLuc7 luciferase with a yield of similar to 3 mg/L of a high purity protein. We demonstrate that MLuc7 produced in insect cells is highly active and extremely thermostable, and is well suited as a secreted reporter when expressed in mammalian cells ensuring higher sensitivity of detection as compared to another Metridia luciferase isoform (MLuc164) which is widely employed in real-time imaging. (C) 2014 Elsevier Inc. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Larionova M. D., Markova S. V., Tikunova N. V., Vysotski E. S.
Заглавие : The smallest isoform of Metridia longa luciferase as a fusion partner for hybrid proteins
Место публикации : Int. J. Mol. Sci.: MDPI AG, 2020. - Vol. 21, Is. 14. - Ст.4971. - С. 1-16. - ISSN 16616596 (ISSN), DOI 10.3390/ijms21144971
Аннотация: Bioluminescent proteins are widely used as reporter molecules in various in vitro and in vivo assays. The smallest isoform of Metridia luciferase (MLuc7) is a highly active, naturally secreted enzyme which, along with other luciferase isoforms, is responsible for the bright bioluminescence of marine copepod Metridia longa. In this study, we report the construction of two variants of a hybrid protein consisting of MLuc7 and 14D5a single-chain antibody to the surface glycoprotein E of tick-borne encephalitis virus as a model fusion partner. We demonstrate that, whereas fusion of a single-chain antibody to either N-or C-terminus of MLuc7 does not affect its bioluminescence properties, the binding site on the single-chain antibody influences its binding capacity. The affinity of 14D5a-MLuc7 hybrid protein (KD = 36.2 nM) where the C-terminus of the single-chain antibody was fused to the N-terminus of MLuc7, appeared to be 2.5-fold higher than that of the reverse, MLuc7-14D5a (KD = 87.6 nM). The detection limit of 14D5a-MLuc7 hybrid protein was estimated to be 45 pg of the recombinant glycoprotein E. Although the smallest isoform of M. longa luciferase was tested as a fusion partner only with a single-chain antibody, it is reasonable to suppose that MLuc7 can also be successfully used as a partner for genetic fusion with other proteins. © 2020 by the authors. Licensee MDPI, Basel, Switzerland.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya V. V., Bashmakova E. E., Kudryavtsev A. N., Vorobjeva M. A., Shatunova E. A., Frank L. A.
Заглавие : The Hybrid Protein ZZ-OL as an Analytical Tool for Biotechnology Research
Колич.характеристики :7 с
Коллективы : Russian FederationRussian Federation [MK-772.2020.4]; Russian Science FoundationRussian Science Foundation (RSF) [16-14-10296]
Место публикации : Russ. J. Bioorg. Chem.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2020. - Vol. 46, Is. 6. - С. 1004-1010. - ISSN 1068-1620, DOI 10.1134/S106816202006014X. - ISSN 1608-330X(eISSN)
Примечания : Cited References:14. - The study was partially supported by a grant of the President of the Russian Federation for Young Scientists, the Candidates of Sciences (project MK-772.2020.4 in the part involving the synthesis and analysis of variants of proteins with melanoma-inhibiting activity) and a grant of the Russian Science Foundation (project no. 16-14-10296 in the part involving the bioluminescence analysis of binding of DNA aptamers to targets).
Аннотация: The gene of the hybrid protein that encodes the double synthetic fragment proZZ of the immunoglobulin-binding domain of protein A of Staphylococcus aureus and apo-obelin joined by a short linker has been cloned. The corresponding hybrid protein has been obtained by expression in Escherichia coli cells. The protein activated with a substrate (coelenterazine) possesses the bioluminescent Ca2+-dependent activity of the photoprotein close to that of recombinant wild-type obelin, and the immunoglobulin-binding ability of protein A. It has been shown that the hybrid can be used as a highly sensitive label to detect antibodies and estimate their affinity and interaction with recombinant proteins, as well as in investigations of other kinds.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GORBAN A.N., MIRKES E.M., POPOVA T.G., SADOVSKY M.G.
Заглавие : THE COMPARATIVE REDUNDANCY OF GENES OF VARIOUS ORGANISMS AND VIRUSES
Колич.характеристики :7 с
Место публикации : Genetika: MEZHDUNARODNAYA KNIGA, 1993. - Vol. 29, Is. 9. - P1413-1419. - ISSN 0016-6758
Примечания : Cited References: 20
Предметные рубрики: CODON
Аннотация: This paper is devoted to the comparative stude of redundancy of genetic texts of various organisms and viruses. To determine the tedundance of a gene, we have introduced the strict measure for that latter. The measure for a text's redundance is the length of restriction of Frequence/Correlation Dictionary of a given genetic text. Frequence/Correlation Dictionary is the ser of all subsequences belang to a given genetic text, accompanied by the frequencies of their occurrence. The restriction length is defined as that one, for which all the subsequences (of that length) are unique. We have found, that genes of human viruses are less redundant, in comparison to those of human genes. Other aspects of a comparative redundance invastigations of the genes are discussed. The problem of the determination of truet intron could be treated by this methodology, as well, as the evolution of genome.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gorban A.N., Mirkes E.M., Popova T.G., Sadovsky M.G.
Заглавие : The comparative redundancy of genes of various organisms and viruses
Место публикации : Genetika. - 1993. - Vol. 29, Is. 9. - P1413-1419. - ISSN 00166758 (ISSN)
Ключевые слова (''Своб.индексиров.''): article--dna content--evolution--genome--nonhuman--restriction mapping--virus--amino acid sequence--comparative study--gene frequency--human--molecular genetics--nucleotide sequence--restriction mapping--virus gene--amino acid sequence--base sequence--comparative study--english abstract--gene frequency--genes, viral--human--molecular sequence data--restriction mapping
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Artamonova V. S., Afanasyev S. A., Bardukov N. V., Golod V. M., Kokodiy S. V., Koulish A. V., Pashkov A. N., Pipoyan S. K., Reshetnikov S. I., Makhrov A. A.
Заглавие : The Center of Origin and Colonization Routes of Noble Salmons of the Genus Salmo (Salmonidae, Actinopterigii)
Место публикации : Doklad. Biochem. Biophys.: Pleiades Publishing, 2020. - Vol. 493, Is. 1. - С. 171-177. - ISSN 16076729 (ISSN), DOI 10.1134/S160767292004002X
Аннотация: Abstract: Genetic diversity and colonization routes of noble salmons were studied using a partial nucleotide sequence of the mitochondrial COI gene. The brown trout S. trutta, which is the most ancient species of the genus, was concluded to originate from the modern southeastern Pontic-Caspian area, which is currently inhabited by members of the subspecies S. trutta oxianus. Migrating westward while the Paratethys was in existence (5–34 million years ago), species of the genus colonized ancient water bodies in the modern Mediterranean basin and formed many isolated populations that survived desiccation of the Mediterranean Sea (5–6 million years ago). The Strait of Gibraltar mediated brown trout migrations to Northern Europe; the subspecies S. trutta trutta belongs to a relatively young phylogenetic lineage of the species. A separate brown trout lineage, currently classified as the subspecies S. trutta labrax, formed most likely in the area of the modern Danube basin, which was a relatively separate part of the Paratethys and was sometimes isolated as the Pannonian Lake. A highly divergent phylogenetic lineage of Atlantic salmon (S. salar) haplotypes originates from a haplotype of the brown trout that inhabited the area of the modern Strait of Gibraltar. © 2020, Pleiades Publishing, Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chang, Dao, Liu, Yangkun, Chen, Yangyang, Hu, Xiaomin, Burov, Andrey, Puzyr, Alexey, Bondar, Vladimir, Yao, Lunguang
Заглавие : Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system
Колич.характеристики :9 с
Коллективы : National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31870917]; program for Innovative Research Team of Science and Technology in University of Henan Province [20IRTSTHN024]; Key Scientific Research Projects of Colleges and Universities in Henan Province of China [18B230008]
Место публикации : BMC Vet. Res.: BMC, 2020. - Vol. 16, Is. 1. - Ст.202. - ISSN 1746-6148(eISSN), DOI 10.1186/s12917-020-02422-3
Примечания : Cited References:30. - This work was financially supported by the National Natural Science Foundation of China (No. 31870917), The program for Innovative Research Team of Science and Technology in University of Henan Province (No. 20IRTSTHN024) and Key Scientific Research Projects of Colleges and Universities in Henan Province of China (No. 18B230008). The funding bodies played no role in the design of the study, the collection, analysis, and interpretation of data and in writing the manuscript.
Предметные рубрики: VIRUS-LIKE PARTICLES
ESCHERICHIA-COLI
GENETIC-ANALYSIS
CPV-VP2
Аннотация: Background Canine parvovirus (CPV) is now recognized as a serious threat to the dog breeding industry worldwide. Currently used CPV vaccines all have their specific drawbacks, prompting a search for alternative safe and effective vaccination strategies such as subunit vaccine. VP2 protein is the major antigen targeted for developing CPV subunit vaccine, however, its production in baculovirus expression system remains challenging due to the insufficient yield. Therefore, our study aims to increase the VP2 protein production by using an improved baculovirus expression system and to evaluate the immunogenicity of the purified VP2 protein in mice. Results The results showed that high-level expression of the full length VP2 protein was achieved using our modified baculovirus expression system. The recombinant virus carrying two copies of VP2 gene showed the highest expression level, with a productivity of 186 mg/L, which is about 1.4-1.6 fold that of the recombinant viruses carrying only one copy. The purified protein reacted with Mouse anti-His tag monoclonal antibody and Rabbit anti-VP2 polyclonal antibody. BALB/c mice were intramuscularly immunized with purified VP2 protein twice at 2 week intervals. After vaccination, VP2 protein could induce the mice produce high level of hemagglutination inhibition antibodies. Conclusions Full length CPV VP2 protein was expressed at high level and purified efficiently. Moreover, it stimulated mice to produce high level of antibodies with hemmaglutination inhibition properties. The VP2 protein expressed in this study could be used as a putative economic and efficient subunit vaccine against CPV infection.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Chang D., Liu Y., Chen Y., Hu X., Burov A., Puzyr A., Bondar V., Yao L.
Заглавие : Study of the immunogenicity of the VP2 protein of canine parvovirus produced using an improved Baculovirus expression system
Место публикации : BMC Vet. Res.: BioMed Central Ltd., 2020. - Vol. 16, Is. 1. - Ст.202. - ISSN 17466148 (ISSN), DOI 10.1186/s12917-020-02422-3
Аннотация: Background: Canine parvovirus (CPV) is now recognized as a serious threat to the dog breeding industry worldwide. Currently used CPV vaccines all have their specific drawbacks, prompting a search for alternative safe and effective vaccination strategies such as subunit vaccine. VP2 protein is the major antigen targeted for developing CPV subunit vaccine, however, its production in baculovirus expression system remains challenging due to the insufficient yield. Therefore, our study aims to increase the VP2 protein production by using an improved baculovirus expression system and to evaluate the immunogenicity of the purified VP2 protein in mice. Results: The results showed that high-level expression of the full length VP2 protein was achieved using our modified baculovirus expression system. The recombinant virus carrying two copies of VP2 gene showed the highest expression level, with a productivity of 186 mg/L, which is about 1.4-1.6 fold that of the recombinant viruses carrying only one copy. The purified protein reacted with Mouse anti-His tag monoclonal antibody and Rabbit anti-VP2 polyclonal antibody. BALB/c mice were intramuscularly immunized with purified VP2 protein twice at 2 week intervals. After vaccination, VP2 protein could induce the mice produce high level of hemagglutination inhibition antibodies. Conclusions: Full length CPV VP2 protein was expressed at high level and purified efficiently. Moreover, it stimulated mice to produce high level of antibodies with hemmaglutination inhibition properties. The VP2 protein expressed in this study could be used as a putative economic and efficient subunit vaccine against CPV infection. © 2020 The Author(s).
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Grodnitskaya I. D., Trusova M. Y., Syrtsov S. N., Koroban N. V.
Заглавие : Structure of microbial communities of peat soils in two bogs in Siberian tundra and forest zones
Место публикации : Microbiology: Maik Nauka Publishing / Springer SBM, 2018. - Vol. 87, Is. 1. - С. 89-102. - ISSN 00262617 (ISSN) , DOI 10.1134/S0026261718010083
Ключевые слова (''Своб.индексиров.''): 16s rrna gene--bacterial diversity--ch4 and co2 emission--cryogenic conditions--methanogenesis--methanotrophy--microbial biomass and chemoorganotroph respiration--oligo-mesotrophic and polygonal bogs--permafrost--subarctic tundra
Аннотация: The structure and functional activity of microbial complexes of a forest oligo-mesotrophic subshrub- grass-moss bog (OMB, Central Evenkiya) and a subshrub-sedge bog in the polygonal tundra (PB, Lena River Delta Samoylovsky Island) was studied. Soil of the forest bog (OMB) differed from that of the polygonal tundra bog (PB) in higher productivity (Corg, Ntotal, P, and K reserves), higher biomass of aerobic chemoorganotrophs (2.0 to 2.6 times), and twice the level of available organic matter. The contribution of microorganisms to the carbon pool was different, with the share of Cmic in Corg 1.4 to 2.5 times higher in PB compared to OMB. Qualitative composition of the methane cycle microorganisms in PB and OMB soils differed significantly. Methanogenic archaea (Euryarchaeota) in the shrub-sedge PB of tundra were more numerous and diverse than in the oligo-mesotrophic bog (OMB) and belonged to six families (Methanomassiliicoccaceae, Methanoregulaceae, Methanobacteriaceae, Methanomicrobiaceaee, Methanosarcinaceae, and Methanotrichaceae), while members of only four families (Methanosarcinacea, Methanobacteriaceae, Methanotrichaceae, and Methanomassiliicoccaceae) were revealed in OMB. In both bogs, methane-oxidizing bacteria belonged to Alphaproteobacteria (II) and Gammaproteobacteria (I). Methanotroph diversity was higher in OMB than in PB. Microbial communities of PB soils had higher potential activity of methanogenesis and methanotrophy compared to those of OMB. Methanogenic and methanotrophic activities in PB were 20 and 2.3 times higher, respectively, than in OMB. © 2018, Pleiades Publishing, Ltd.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakova O.V., Gladyshev M.I., Rozanov A.S., Peltek S.E., Trusova M.Y.
Заглавие : Spatial biodiversity of bacteria along the largest Arctic river determined by next-generation sequencing
Место публикации : FEMS Microbiol. Ecol. - 2014. - Vol. 89, Is. 2. - С. 442-450. - ISSN 15746941 (ISSN) , DOI 10.1111/1574-6941.12355
Ключевые слова (''Своб.индексиров.''): 16s rrna gene--bacterial community--diversity--yenisei river--actinobacteria--bacteria (microorganisms)--cyanobacteria--proteobacteria
Аннотация: The biodiversity of bacterial communities along the Yenisei River at section c. 1800 km was studied using next-generation sequencing of 16S rRNA genes and common biodiversity indices. Overall, 3022 unique operational taxonomic units were identified. Actinobacteria and Proteobacteria were the dominant phyla at all sampling sites. The highest alpha-diversity values were found in the middle section of the studied river. The beta-diversity of bacterial assemblages in the river was related to the surrounding landscape (biome): three distinctly different bacterial assemblages occurred in sections of the river, situated in mountain taiga, plain taiga and in a region of permafrost, covered by forest-tundra and tundra. Tributaries arising from these different landscapes likely contributed substantially to the variations of Yenisei bacterial communities. In contrast to a prediction of the river continuum concept, the proportion of photoautotrophic Cyanobacteria in bacterial assemblages did not increase downstream, but peaked at the middle section. © 2014 Federation of European Microbiological Societies.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakova O.V., Gladyshev M.I., Rozanov A.S., Peltek S.E., Trusova M.Y.
Заглавие : Spatial biodiversity of bacteria along the largest Arctic river determined by next-generation sequencing
Колич.характеристики :9 с
Коллективы : Attracting Leading Scientists to Russian Educational Institutions Program of the Russian Federation [11.G34.31.0014]; Siberian Federal University
Место публикации : FEMS Microbiol. Ecol.: WILEY-BLACKWELL, 2014. - Vol. 89, Is. 2. - С. 442-450. - ISSN 0168-6496, DOI 10.1111/1574-6941.12355. - ISSN 1574-6941
Примечания : Cited References: 36. - This work was supported by the Attracting Leading Scientists to Russian Educational Institutions Program of the Russian Federation, agreement 11.G34.31.0014, and by the project G-1 of Siberian Federal University, carried out according to Federal tasks of the Ministry of Education and Science of Russian Federation.
Предметные рубрики: DISSOLVED ORGANIC-MATTER
INLAND WATERS
CARBON
BACTERIOPLANKTON
COMMUNITY
GREENGENES
ECOSYSTEM
RESERVOIR
PATTERNS
PRIMERS
Ключевые слова (''Своб.индексиров.''): bacterial community--diversity--16s rrna gene--yenisei river
Аннотация: The biodiversity of bacterial communities along the Yenisei River at section c. 1800 km was studied using next-generation sequencing of 16S rRNA genes and common biodiversity indices. Overall, 3022 unique operational taxonomic units were identified. Actinobacteria and Proteobacteria were the dominant phyla at all sampling sites. The highest alpha-diversity values were found in the middle section of the studied river. The beta-diversity of bacterial assemblages in the river was related to the surrounding landscape (biome): three distinctly different bacterial assemblages occurred in sections of the river, situated in mountain taiga, plain taiga and in a region of permafrost, covered by forest-tundra and tundra. Tributaries arising from these different landscapes likely contributed substantially to the variations of Yenisei bacterial communities. In contrast to a prediction of the river continuum concept, the proportion of photoautotrophic Cyanobacteria in bacterial assemblages did not increase downstream, but peaked at the middle section.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Wu Y. -T., Chiang P. -W., Tandon K., Rogozin D. Y., Degermendzhy A. G., Tang S. -L.
Заглавие : Single-cell genomics-based analysis reveals a vital ecological role of thiocapsa sp. LSW in the meromictic Lake Shunet, Siberia
Место публикации : Microb. Genomics: Microbiology Society, 2021. - Vol. 7, Is. 12. - Ст.000712. - ISSN 20575858 (ISSN), DOI 10.1099/mgen.0.000712
Аннотация: Meromictic lakes usually harbour certain prevailing anoxygenic phototrophic bacteria in their anoxic zone, such as the purple sulfur bacterium (PSB) Thiocapsa sp. LSW (hereafter LSW) in Lake Shunet, Siberia. PSBs have been suggested to play a vital role in carbon, nitrogen and sulfur cycling at the oxic–anoxic interface of stratified lakes; however, the ecological significance of PSBs in the lake remains poorly understood. In this study, we explored the potential ecological role of LSW using a deep-sequencing analysis of single-cell genomics associated with flow cytometry. An approximately 2.7 Mb draft genome was obtained based on the co-assembly of five single-cell genomes. LSW might grow photolithoautotrophically and could play putative roles not only as a carbon fixer and diazotroph, but also as a sulfate reducer/oxidizer in the lake. This study provides insights into the potential ecological role of Thiocapsa sp. in meromictic lakes. © 2021 The Authors.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bashmakova, Eugenia E., Krasitskaya, Vasilisa V., Frank, Ludmila A.
Заглавие : Simultaneous Genotyping of Four Single Nucleotide Polymorphisms Associated with Risk Factors of Hemostasis Disorders
Колич.характеристики :7 с
Коллективы : Russian Science Foundation [14-14-01119]
Место публикации : Comb. Chem. High Throughput Screen: BENTHAM SCIENCE PUBL LTD, 2015. - Vol. 18, Is. 10. - С. 930-936. - ISSN 1386-2073, DOI 10.2174/1386207318666150917095903. - ISSN 1875-5402(eISSN)
Примечания : Cited References:20. - The study was supported by the grant 14-14-01119 of the Russian Science Foundation.
Предметные рубрики: ALLELE-SPECIFIC PCR
FACTOR-V-LEIDEN
BIOLUMINESCENT IMMUNOASSAY
Ключевые слова (''Своб.индексиров.''): snp detection--pext reaction--photoprotein obelin--bioluminescent--microassay--multiplex pcr
Аннотация: Multiplex simultaneous genotyping technique was developed for four polymorphisms in genes coding for blood coagulation factors and homocysteine metabolism which are considered as thrombophilia related mutations: FV Leiden, FII G20210A, MTHFR C677T, and FVII G10976A. It is based on primer extension reaction with the following bioluminescent solid-phase microassay. At that, two different in bioluminescence obelin mutants were applied to simultaneous detection of two gene allelic variants. The assay is carried out in microtiter plate format and provides fast and reliable genotyping of four single nucleotide polymorphisms in four different genes within 2.5 hours. A large number of clinical samples were analyzed and the obtained results were found to be in complete correlation with those obtained by using conventional RT-PCR techniques.
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bashmakova E. E., Krasitskaya V. V., Frank L. A.
Заглавие : Simultaneous genotyping of four single nucleotide polymorphisms associated with risk factors of hemostasis disorders
Место публикации : Comb. Chem. High Throughput Screen. - 2015. - Vol. 18, Is. 10. - С. 930-936. - ISSN 13862073 (ISSN)
Ключевые слова (''Своб.индексиров.''): bioluminescent microassay--multiplex pcr--pext reaction--photoprotein obelin--snp detection
Аннотация: Multiplex simultaneous genotyping technique was developed for four polymorphisms in genes coding for blood coagulation factors and homocysteine metabolism which are considered as thrombophilia related mutations: FV Leiden, FII G20210A, MTHFR C677T, and FVII G10976A. It is based on primer extension reaction with the following bioluminescent solid-phase microassay. At that, two different in bioluminescence obelin mutants were applied to simultaneous detection of two gene allelic variants. The assay is carried out in microtiter plate format and provides fast and reliable genotyping of four single nucleotide polymorphisms in four different genes within 2.5 hours. A large number of clinical samples were analyzed and the obtained results were found to be in complete correlation with those obtained by using conventional RT-PCR techniques. © 2015 Bentham Science Publishers.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : ILLARIONOV B.A., BONDAR V.S., ILLARIONOVA V.A., VYSOTSKI E.S.
Заглавие : SEQUENCE OF THE CDNA-ENCODING THE CA2+-ACTIVATED PHOTOPROTEIN OBELIN FROM THE HYDROID POLYP OBELIA-LONGISSIMA
Место публикации : Gene: ELSEVIER SCIENCE BV, 1995. - Vol. 153, Is. 2. - С. 273-274. - 2. - ISSN 0378-1119, DOI 10.1016/0378-1119(94)00797-V
Примечания : Cited References: 6
Предметные рубрики: CA-2+-ACTIVATED PHOTOPROTEIN
AEQUORIN
CLONING
Ключевые слова (''Своб.индексиров.''): bioluminescence--calcium--gene--plasmid--marine coelenterates
Аннотация: A cDNA clone encoding the Ca2+-activated photoprotein, obelin (Obl), from Obelia longissima was sequenced. The nucleotide (nt) sequence contained two long overlapping open reading frames (ORFs), one of which encoded apoobelin (apoObl). The deduced amino acid (aa) sequence of apoObl revealed that this 195-aa protein has three EF-hand structures that are characteristic for Ca2+-binding domains. Strong aa homology was shown among apoObl, apoaequorin and apoclytin. The second ORF present in the obl cDNA consists of 139 codons and encodes a very basic protein with a calculated pI of 10.56 and a molecular mass of 16153 Da.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GORBAN A.N., POPOVA T.G., SADOVSKII M.G.
Заглавие : REDUNDANCY OF GENETIC TEXTS
Колич.характеристики :8 с
Место публикации : Mol. Biol.: PLENUM PUBL CORP, 1994. - Vol. 28, Is. 2. - P206-213. - ISSN 0026-8933
Примечания : Cited References: 14
Ключевые слова (''Своб.индексиров.''): gene--correlation--redundancy
Аннотация: A new method is proposed for measuring and comparing the redundancy of genetic texts. It is based on compiling for a given text a frequency/correlation dictionary encompassing all the words (subsequences) of length from 1 to N encountered in the text (N is text length); the measure of redundancy is the minimal length at which all words are unique. Preliminary results are reported for different genetic texts.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : POPOVA T.G., SADOVSKII M.G.
Заглавие : REDUNDANCY OF EUKARYOTIC DNA DECREASES AFTER EXCISION OF INTRONS
Колич.характеристики :5 с
Место публикации : Mol. Biol.: PLENUM PUBL CORP, 1995. - Vol. 29, Is. 3. - P281-285. - ISSN 0026-8933
Примечания : Cited References: 23
Предметные рубрики: SEQUENCES
Ключевые слова (''Своб.индексиров.''): exon--intron--word--frequency--redundancy
Аннотация: The article is devoted to analysis of the internal gene structure with respect to redundancy of exons and introns. Human genes with precisely determined exon-intron structure were studied. Redundancy of each exon and intron within a certain gene was measured. In the analyzed human genes, introns were more redundant than exons. Redundancy was determined as the minimal length of a word (oligonucleotide) that is present as a single copy within the nucleotide sequence studied. Mechanisms are discussed that lead to disturbances in the relationships found between the redundancies of exons and introns.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : GITELZON I.I., SANDALOVA T.P.
Заглавие : PROSPECTS FOR APPLICATION OF BIOLUMINESCENCE METHOD IN MEDICINE
Колич.характеристики :5 с
Место публикации : VESTNIK AKADEMII MEDITSINSKIKH NAUK SSSR: IZD VO MEDITSINA, 1990. - Is. 9. - С. 31-35. - ISSN 0002-3027
Примечания : Cited References: 41
Предметные рубрики: AMINO-ACID SEQUENCE
NUCLEOTIDE-SEQUENCE
VIBRIO-HARVEYI
BACTERIAL LUCIFERASE
FIREFLY LUCIFERASE
SUBUNIT
CELLS
GENE
PHOTOPROTEINS
EXPRESSION
Аннотация: Major advances in the development and application of the bioluminescent analysis to detect certain biologically active substances are discussed. The main merit of the method lies in its high sensitivity and specificity along with its simplicity and rapid performance. The available methodologies allow for detection of substances of varying nature: Ca2+, ATP, FMN, NAD(P), long-chain aldehydes, ATP- and NAD(P)-dependent enzymes and their substrates, many xenobiotics and antibiotics, and mutagens. The bioluminescence methodologies may be widely applied in clinical laboratory diagnosis.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Petushkov V.N., Gibson B.G., Lee J.
Заглавие : Properties of recombinant fluorescent proteins from Photobacterium leiognathi and their interaction with luciferase intermediates
Место публикации : Biochemistry. - 1995. - Vol. 34, Is. 10. - С. 3300-3309. - ISSN 00062960 (ISSN)
Ключевые слова (''Своб.индексиров.''): luciferase--recombinant protein--article--ligand binding--nonhuman--priority journal--protein isolation--protein protein interaction--protein stability--vibrionaceae--bacterial proteins--binding sites--carrier proteins--circular dichroism--flavin mononucleotide--fluorescence polarization--genes, bacterial--kinetics--ligands--luciferase--luminescence--molecular sequence data--photobacterium--recombinant proteins--spectrophotometry--support, u.s. gov't, p.h.s.--photobacterium leiognathi--vibrionaceae
Аннотация: Ligand binding and luciferase interaction properties of the recombinant protein corresponding to the lumazine protein gene (EMBL X56534) of Photobacterium leiognathi have been determined by fluorescence dynamics, circular dichroism, gel filtration, and SDS-PAGE. Scatchard analysis of a fluorescence titration shows that the apoprotein possess one binding site, and at 30В°C the KdS (?M) are as follows: 6,7-dimethyl-8-ribityllumazine, 0.26; riboflavin, 0.53; and much more weakly bound FMN, 30. All holoproteins are highly fluorescent and have absorption spectra distinct from each other and from the free ligands. The longest wavelength absorption maxima are, respectively (nm, 2В°C), 420,463, and 458. Ligand binding produces no change in the far-UV circular dichroism; all have mean residual ellipticity at 210 nm of -6500 deg cm2 dmol-1, the same as the native protein. However, in the bioluminescence reaction only the lumazine holoprotein shows a bioluminescence effect. Fluorescence emission anisotropy decay was used to establish that none of these holoproteins complexed with native luciferase and that the lumazine protein alone formed a 1:1 complex with the luciferase hydroxyflavin fluorescent transient and the luciferase peroxyflavin intermediates, revealed by a dominant channel of anisotropy loss, with rotational correlation time of 2.5 ns, and attributed to excitation transfer from the luciferase flavin donor to the acceptor, the lumazine ligand. The complex stability was sufficient to allow its isolation by FPLC gel filtration and verification by SDS-PAGE. These methods also confirmed the absence of interaction of the holoflavoproteins.
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