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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zotina T.A., Kalachova G.S., Bolsunovsky A.Ya., Degermendzhy A.G.
Заглавие : 241Am distribution in the biomass of freshwater macrophytes
Место публикации : Doklady Biological Sciences. - 2008. - Vol. 421, Is. 1. - С. 254-256. - ISSN 00124966 (ISSN) , DOI 10.1134/S0012496608040108
Ключевые слова (''Своб.индексиров.''): americium--carbohydrate--cellulose--lipid--nitrogen--polysaccharide--vegetable protein--article--biomass--bryopsida--cell membrane--cell wall--chemistry--cytoplasm--food chain--growth, development and aging--hydrocharitaceae--metabolism--americium--biomass--bryopsida--carbohydrates--cell membrane--cell wall--cellulose--cytoplasm--food chain--hydrocharitaceae--lipids--nitrogen--plant proteins--polysaccharides
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Zhila N., Kiselev E., Shishatskaya E.
Заглавие : A study of synthesis and properties of poly-3-hydroxybutyrate/diethylene glycol copolymers
Место публикации : Biotechnol. Prog.: John Wiley and Sons Inc., 2016. - Vol. 32, Is. 4. - С. 1017-1028. - ISSN 87567938 (ISSN) , DOI 10.1002/btpr.2267
Ключевые слова (''Своб.индексиров.''): biocompatibility--biosynthesis--molecular weight--physicochemical and mechanical properties--poly(3-hydroxybutyrate)/diethylene glycol copolymers--biochemistry--biocompatibility--biosynthesis--cell culture--cell membranes--cells--cytology--fatty acids--glycols--molecular weight--american institute of chemical engineers--biological properties--chemical compositions--cytoplasmic membrane--degree of saturations--physico-chemical and mechanical properties--physiological effects--poly-3-hydroxybutyrate--biomechanics
Аннотация: This study investigates synthesis of poly(3-hydroxybutyrate)/diethylene glycol copolymers (P3HB/DEG) by Cupriavidus eutrophus B-10646 cells as related to DEG concentration in the medium and the time when it is added to the culture of cells synthesizing P3HB. The study determines the limits of physiological effect of DEG on C. eutrophus cells, showing that at DEG concentrations above 30 g/L, it inhibits cell growth, decreasing cell concentration and total P3HB/DEG yield and inducing an increase in the degree of saturation of fatty acids in lipids of cell cytoplasmic membrane. A series of copolymers containing different molar fractions of DEG (between 0.13 and 3.0 mol%) have been synthesized and their physicochemical, physical/mechanical, and biological properties have been investigated as related to the chemical composition and proportions of DEG monomers of the polymers. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:1017–1028, 2016. © 2016 American Institute of Chemical Engineers
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Kalacheva G.S., Zhila N.O., Gladyshev M.I., Volova T.G.
Заглавие : A temperature dependence of the intra- and extracellular fatty-acid composition of green algae and cyanobacterium
Колич.характеристики :7 с
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA, 2003. - Vol. 50, Is. 3. - P374-380. - ISSN 1021-4437, DOI 10.1023/A:1023830405898
Примечания : Cited References: 31
Предметные рубрики: SURFACE-FILM
LIPIDS
WATER
BLOOM
SEA
BAY
Ключевые слова (''Своб.индексиров.''): green algae--cyanobacterium--lipids--fatty acids--secretion
Аннотация: The effect of ambient temperature on the composition of intracellular fatty acids and the release of free fatty acids (FFA) into a medium by cyanobacterium Spirulina platensis and eukaryotic microalgae, Chlorella vulgaris and Botryococcus braunii, was studied using their batch cultures. It was found that all the species studied, regardless of their taxonomic status, responded to the temperature regime by similar changes in their intracellular fatty acid composition: the relative content of more unsaturated fatty acids decreased with the elevation of temperature. At the same time, in the prokaryote, this temperature shift blocked, first of all, the elongation of 16:0 to 18:0 and then their further desaturation. In eukaryotes, the change in the desaturation of dienoic to trienoic fatty acids was the most pronounced process. The ratio of dienoic to trienoic fatty acids remained almost unchanged in S. platensis. The relative content of extracellular unsaturated FFA increased in the prokaryotic organism S. platensis at a higher temperature. But no significant changes in the composition of extracellular unsaturated FFA were detected in eukaryotic algae upon temperature elevation.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Kalacheva G.S., Zhila N.O., Gladyshev M.I., Volova T.G.
Заглавие : A Temperature Dependence of the Intra- and Extracellular Fatty-Acid Composition of Green Algae and Cyanobacterium
Место публикации : Russian Journal of Plant Physiology. - 2003. - Vol. 50, Is. 3. - С. 374-380. - ISSN 10214437 (ISSN) , DOI 10.1023/A:1023830405898
Ключевые слова (''Своб.индексиров.''): cyanobacterium--fatty acids--green algae--lipids--secretion--algae--bacteria (microorganisms)--botryococcus braunii--chlorella vulgaris--chlorophyta--eukaryota--prokaryota--spirulina--spirulina platensis
Аннотация: The effect of ambient temperature on the composition of intracellular fatty acids and the release of free fatty acids (FFA) into a medium by cyanobacterium Spirulina platensis and eukaryotic microalgae, Chlorella vulgaris and Botryococcus braunii, was studied using their batch cultures. It was found that all the species studied, regardless of their taxonomic status, responded to the temperature regime by similar changes in their intracellular fatty acid composition: the relative content of more unsaturated fatty acids decreased with the elevation of temperature. At the same time, in the prokaryote, this temperature shift blocked, first of all, the elongation of 16: 0 to 18:0 and then their further desaturation. In eukaryotes, the change in the desaturation of dienoic to trienoic fatty acids was the most pronounced process. The ratio of dienoic to trienoic fatty acids remained almost unchanged in S. platensis. The relative content of extracellular unsaturated FFA increased in the prokaryotic organism S. platensis at a higher temperature. But no significant changes in the composition of extracellular unsaturated FFA were detected in eukaryotic algae upon temperature elevation.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : KALACHEVA G.S., VOLOVA T.G., TRUBACHEV I.N.
Заглавие : ANALYSIS OF THE COMPOSITION OF LIPIDS FROM CARBOXYDOBACTERIA
Колич.характеристики :6 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1980. - Vol. 49, Is. 5. - P595-600. - ISSN 0026-2617
Примечания : Cited References: 17
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : KALACHEVA G.S., VOLOVA T.G., TRUBACHEV I.N.
Заглавие : ANALYSIS OF THE COMPOSITION OF LIPIDS FROM CARBOXYDOBACTERIA
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1980. - Vol. 49, Is. 5. - С. 595-600. - 6. - ISSN 0026-2617
Примечания : Cited References: 17
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Altukhova O.V.
Заглавие : Autotrophic synthesis of polyhydroxyalkanoates by the bacteria Ralstonia eutropha in the presence of carbon monoxide
Место публикации : Applied Microbiology and Biotechnology. - 2002. - Vol. 58, Is. 5. - С. 675-678. - ISSN 01757598 (ISSN) , DOI 10.1007/s00253-002-0941-8
Ключевые слова (''Своб.индексиров.''): 3 hydroxybutyric acid--acetoacetyl coenzyme a reductase--acetyl coenzyme a acyltransferase--beta hydroxyvalerate--butyrate dehydrogenase--carbon monoxide--electrolyte--hydrogen--oxidoreductase--poly(3 hydroxybutyric acid)--poly(3 hydroxybutyric acid)synthase--polyhydroxyalkanoic acid--polymer--unclassified drug--valeric acid--bacterium--article--autotrophy--bacterial growth--bacterial strain--biomass production--controlled study--crystallization--enzyme activity--molecular weight--nonhuman--synthesis--temperature--wautersia eutropha--carbon monoxide--culture media--cupriavidus necator--fatty acids--lipids--polyesters--bacteria (microorganisms)--negibacteria--ralstonia--wautersia eutropha
Аннотация: It has been found that the carbon monoxide (CO)-resistant strain of the hydrogen bacteria Ralstonia eutropha B5786 is able to synthesise polyhydroxy-alkanoates (PHAs) in the presence of CO under autotrophic conditions. This strain, grown on model gas mixtures containing 5-25% CO (v/v), accumulates up to 70-75% (of absolutely dry matter) PHA, without significant variation in the yield coefficient on hydrogen. No suppression of the activities of the key enzymes of PHA synthesis (?-ketothiolase, acetoacetyl-CoA-reductase, butyrate dehydrogenase and poly-3-hydroxybutyrate synthase) was recorded. The PHA synthesised is a copolymer containing mostly ?-hydroxybutyrate (more than 99 mol%) with trace amounts of ?-hydroxyvalerate. The investigated properties of the polymer (molecular weight, crystallinity, temperature characteristics) do not differ from those of the polymer synthesised on electrolytic hydrogen.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kuchkina A., Gladyshev M.I., Sushchik N.N., Kravchuk E.S., Kalachova G.S.
Заглавие : Biodiesel production from sediments of a eutrophic reservoir
Место публикации : Biomass and Bioenergy. - 2011. - Vol. 35, Is. 5. - С. 2280-2284. - ISSN 09619534 (ISSN) , DOI 10.1016/j.biombioe.2011.02.047
Ключевые слова (''Своб.индексиров.''): biodiesel--dredging sediments--eutrophic reservoir--fatty acids--lipids--bio-diesel fuel--biodiesel production--cetane number--dry weight--en 14214--eutrophic reservoirs--heat of combustion--high price--iodine number--is costs--lake restoration--lipid content--production cost--siberia--anoxic sediments--biodiesel--costs--diesel fuels--eutrophication--fatty acids--iodine--lipids--reservoirs (water)--sedimentology--synthetic fuels--thermochemistry--dredging--biofuel--combustion--dredging--environmental restoration--eutrophic environment--iodine--lacustrine deposit--lipid--production cost--reservoir--bugach reservoir--krasnoyarsk [russian federation]--russian federation
Аннотация: Sediments from eutrophic reservoir Bugach (Siberia, Russia) were tested for possibility to produce biodiesel. We supposed that the sediments could be a promising biodiesel producer. The major reason of high price of biodiesel fuel is cost of a raw material. The use of dredging sediments for biodiesel production reduces production costs, because the dredging sediments are by-products which originated during lake restoration actions, and are free of cost raw materials. Lipid content in sediments was 0.24% of dry weight. To assess the potential of from sediments as a substitute of diesel fuel, the properties of the biodiesel such as cetane number, iodine number and heat of combustion were calculated. All of this parameters complied with limits established by EN 14214 and EN 14213 related to biodiesel quality. В© 2011 Elsevier Ltd.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N. O., Kalacheva G. S., Fokht V. V., Bubnova S. S., Volova T. G.
Заглавие : Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by cupriavidus necator B-10646 from mixtures of oleic acid and 3-hydroxyvalerate precursors
Место публикации : J. Sib. Fed. Univ. - Biol.: Siberian Federal University, 2020. - Vol. 13, Is. 3. - С. 331-341. - ISSN 19971389 (ISSN), DOI 10.17516/1997-1389-0320
Аннотация: Polyhydroxyalkanoates have attracted much attention as biodegradable alternative to petroleum-based synthetic plastics. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] copolymer is one of the best characterized PHA copolymers because of its high commercial potential. However, commercial use of PHAs has been limited by their high price. One approach to reducing the cost of PHA production is to use inexpensive carbon sources (fatty acids, plant oils, etc.). The aim of this work was to study synthesis of P(3HB-co-3HV) by the Cupriavidus necator B-10646 bacterium grown on oleic acid and different biochemical precursors of 3HV. Bacterial cells were grown for 72 h at 30°C and 200 rpm on an incubator shaker. Salts of propionic or valeric acids were used as precursors of 3HV. The content and the composition of the polymer were determined by gas chromatography of fatty acid methyl esters. Lipids and polymer were extracted from biomass using the method of Folch. The addition of potassium propionate and valerate did not inhibit bacterial growth and polymer synthesis, the cell concentration and polymer content reaching 9.3-9.5 g/L and 80-83%, respectively. The addition of potassium valerate or propionate led to the synthesis of (P(3HB-co-3HV)) copolymer containing 21.2 and 14.3 mol% of 3HV, respectively. The number average molecular weight (Mn) of the polymer synthesized by the bacterium on oleic acid alone was 220 kDa; the polydispersity of the polymer was 3.5. The polymer synthesized in the presence of potassium valerate and propionate was characterized by a lower Mn (156-178 kDa) and a higher polydispersity of the polymer (4.4-4.9). The main fatty acids (FA) of intracellular lipids were oleic (33.26% of the total FA) and palmitic acid (27.48% of the total FA). The addition of potassium propionate or valerate did not cause any significant changes in the composition of the FA of intracellular lipids of the strain studied. This study demonstrates the ability of C. necator B-10646 to synthesize P(3HB-co-3HV) from mixtures of oleic acid and 3HV precursors. The data obtained can be used to develop and implement an economically feasible process of the P(3HB-co-3HV) production. © Siberian Federal University. All rights reserved.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : KALACHEVA G.S., VYSOTSKII E.S.
Заглавие : CHANGE IN LIPIDS AND THEIR FATTY-ACID COMPOSITION IN BATCH CULTURING OF LUMINESCENT BACTERIA
Колич.характеристики :5 с
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1984. - Vol. 53, Is. 6. - P756-760. - ISSN 0026-2617
Примечания : Cited References: 16
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : KALACHEVA G.S., VYSOTSKII E.S.
Заглавие : CHANGE IN LIPIDS AND THEIR FATTY-ACID COMPOSITION IN BATCH CULTURING OF LUMINESCENT BACTERIA
Место публикации : Microbiology: MAIK NAUKA/INTERPERIODICA, 1984. - Vol. 53, Is. 6. - С. 756-760. - 5. - ISSN 0026-2617
Примечания : Cited References: 16
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kalacheva G.S., Vysotsky E.S.
Заглавие : Changes of lipids and their fatty acid composition during batch cultivation of the luminescent bacterium
Место публикации : Mikrobiologiya. - 1984. - Vol. 53, Is. 6. - С. 932-937. - ISSN 00263656 (ISSN)
Ключевые слова (''Своб.индексиров.''): fatty acid--lipid--bacterium--bioluminescence--nonhuman
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T.G., Kalacheva G.S., Zhyla N.O.
Заглавие : Characteristics of lipids from green alga Botryococcus braunii synthesizing liquid hydrocarbons.
Место публикации : Doklady Biological Sciences. - 2000. - Vol. 370, Is. 1-6. - С. 96-99. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): fatty acid--hydrocarbon--lipid--article--biomass--chemistry--green alga--metabolism--algae, green--biomass--fatty acids--hydrocarbons--lipids
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15.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Moskvichova A.V., Makhutova O.N., Kalachova G.S.
Заглавие : Comparison of fatty acid composition in major lipid classes of the dominant benthic invertebrates of the Yenisei river
Колич.характеристики :12 с
Место публикации : Comp. Biochem. Physiol. B-Biochem. Mol. Biol.: ELSEVIER SCIENCE INC, 2003. - Vol. 134, Is. 1. - P111-122. - ISSN 1096-4959, DOI 10.1016/S1096-4959(02)00191-4
Примечания : Cited References: 30
Предметные рубрики: LAKE-ERKEN
BIOMARKERS
BACTERIA
MARKERS
DIET
FISH
N-3
Ключевые слова (''Своб.индексиров.''): fatty acids--triacylglycerols--polar lipids--gammarids--aquatic larvae of insects
Аннотация: The composition and content of fatty acids (FAs) in total lipids, triacylglycerols (TAG) and polar lipids (PL) in dominant groups of benthic invertebrates: gammarids (Gammaridae, Amphipoda), chironomid larvae (Chironomidae, Diptera), caddisfly larvae (Trichoptera) and mayfly larvae (Ephemeroptera) were studied in the Yenisei river. For the first time data on the FA composition of species belonging to Trichoptera (Insecta) are presented. The groups of aquatic insect larvae and gammarids weakly differed in total content of essential polyunsaturated fatty acids (PUFAs). Hence, the strong invasion of gammarids which occurred in the last decades in the Yenisei river should not result in a decrease in potential yield of essential PUFA in the ecosystem and corresponding decrease in food resource quality for fish in respect to PUFA content. Significant differences in biomarker FAs in TAG were found which correlated to specific food sources. Different levels of long-chain PUFA in PL of the invertebrates are discussed in relation to the genetic ability of particular taxa to form these FAs. (C) 2002 Elsevier Science Inc. All rights reserved.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Yurchenko Y.A., Gladyshev M.I., Belevich O.E., Kalachova G.S., Kolmakova A.A.
Заглавие : Comparison of fatty acid contents and composition in major lipid classes of larvae and adults of mosquitoes (Diptera: Culicidae) from a steppe region
Место публикации : Insect Science. - 2013. - Vol. 20, Is. 5. - С. 585-600. - ISSN 16729609 (ISSN) , DOI 10.1111/j.1744-7917.2012.01582.x
Ключевые слова (''Своб.индексиров.''): amphibiotic insects--essential fatty acids--metamorphosis--mosquitoes--short-chain polyunsaturated fatty acids
Аннотация: Emerging aquatic insects, including mosquitoes, are known to transfer to terrestrial ecosystems specific essential biochemicals, such as polyunsaturated fatty acids (PUFA). We studied fatty acid (FA) composition and contents of dominant mosquito populations (Diptera: Culicidae), that is, Anopheles messeae, Ochlerotatus caspius, Oc. flavescens, Oc. euedes, Oc. subdiversus, Oc. cataphylla, and Aedes cinereus, inhabited a steppe wetland of a temperate climate zone to fill up the gap in their lipid knowledge. The polar lipid and triacylglycerol fractions of larvae and adults were compared. In most studied mosquito species, we first found and identified a number of short-chain PUFA, for example, prominent 14:2n-6 and 14:3n-3, which were not earlier documented in living organisms. These PUFA, although occurred in low levels in adult mosquitoes, can be potentially used as markers of mosquito biomass in terrestrial food webs. We hypothesize that these acids might be synthesized (or retroconverted) by the mosquitoes. Using FA trophic markers accumulated in triacylglycerols, trophic relations of the mosquitoes were accessed. The larval diet comprised green algae, cryptophytes, and dinoflagellates and provided the mosquitoes with essential n-3 PUFA, linolenic, and eicosapentaenoic acids. As a result, both larvae and adults of the studied mosquitoes had comparatively high content of the essential PUFA. Comparison of FA proportions in polar lipids versus storage lipids shown that during mosquito metamorphosis transfer of essential eicosapentaenoic and arachidonic acids from the reserve in storage lipids of larvae to functional polar lipids in adults occurred. В© 2012 Institute of Zoology, Chinese Academy of Sciences.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik, Nadezhda N., Makhutova, Olesia N., Rudchenko, Anastasia E., Glushchenko, Larisa A., Shulepina, Svetlana P., Kolmakova, Anzhelika A., Gladyshev, Michail I.
Заглавие : Comparison of Fatty Acid Contents in Major Lipid Classes of Seven Salmonid Species from Siberian Arctic Lakes
Колич.характеристики :18 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-10001]
Место публикации : Biomolecules: MDPI, 2020. - Vol. 10, Is. 3. - Ст.419. - ISSN 2218-273X(eISSN), DOI 10.3390/biom10030419
Примечания : Cited References:60. - This research was funded by the Russian Science Foundation, grant number 16-14-10001.
Предметные рубрики: LONG-CHAIN
FISH
QUALITY
OMEGA-3
MARINE
WILD
TISSUE
FOOD
Аннотация: Long-chain omega-3 polyunsaturated fatty acids (LC-PUFA) essential for human nutrition are mostly obtained from wild-caught fish. To sustain the LC-PUFA supply from natural populations, one needs to know how environmental and intrinsic factors affect fish fatty acid (FA) profiles and contents. We studied seven Salmoniformes species from two arctic lakes. We aimed to estimate differences in the FA composition of total lipids and two major lipid classes, polar lipids (PL) and triacylglycerols (TAG), among the species and to evaluate LC-PUFA contents corresponding to PL and TAG in muscles. Fatty acid profiles of PL and TAG in all species were characterized by the prevalence of omega-3 LC-PUFA and C16-C18 monoenoic FA, respectively. Fish with similar feeding spectra were identified similarly in multivariate analyses of total lipids, TAG and PL, due to differences in levels of mostly the same FA. Thus, the suitability of both TAG and total lipids for the identification of the feeding spectra of fish was confirmed. All species had similar content of LC-PUFA esterified as PL, 1.9-3.5 mg g(-1), while the content of the TAG form strongly varied, from 0.9 to 9.8 mg g(-1). The LC-PUFA-rich fish species accumulated these valuable compounds predominately in the TAG form.
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N.O., Kalacheva G.S., Volova T.G.
Заглавие : Effect of nitrogen limitation on the growth and lipid composition of the green alga Botryococcus braunii Kutz IPPAS H-252
Место публикации : Russian Journal of Plant Physiology. - 2005. - Vol. 52, Is. 3. - С. 311-319. - ISSN 10214437 (ISSN) , DOI 10.1007/s11183-005-0047-0
Ключевые слова (''Своб.индексиров.''): botryococcus--lipids--nitrogen limitation--algae--botryococcus--botryococcus braunii--chlorophyta
Аннотация: The effect of nitrogen limitation in a medium on the composition of intracellular lipids in the alga Botryococcus braunii Kutz IPPAS H-252 in the course of culture development was investigated. Under the conditions of nitrogen limitation, the alga under investigation accumulated lipids as triacylglycerols, and this process was accompanied by substantial changes in the total fatty acid (FA) composition, which were manifested in a decrease in trienoic acids (from 52.8-57.2 to 19.5-24.7% of total FAs) and an increase in the content of oleic (from 1.1-1.2 to 17.1-24.4%) and saturated (from 23.7-26.0 to 32.9-46.1%) acids. In the control culture, the directionality of FA redistribution was less marked, and these changes were noticed at the later stages of culture development. Under nitrogen limitation, marked changes in the FA composition of polar lipids occurred by the 13th day, and they were characterized by an increase in the content of saturated acids (up to 76.8%) and a dramatic decrease in the content of all polyenoic acids (up to 6.8%). The changes in the FA composition of triacylglycerols were noticed as early as by the 7th day; these changes consisted in an increase in the content of oleic acid, and its high content (28.4-38.4%) was maintained up to the end of culturing. In the control culture, triacylglycerols with a high content of oleic acid were found by the 13th day, although, by this time, the content of total lipids and triacylglycerols did not change. В© 2005 MAIK "Nauka/Interperiodica".
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zhila N.O., Kalacheva G.S., Volova T.G.
Заглавие : Effect of salinity on the biochemical composition of the alga Botryococcus braunii Kutz IPPAS H-252
Место публикации : Journal of Applied Phycology. - 2011. - Vol. 23, Is. 1. - С. 47-52. - ISSN 09218971 (ISSN) , DOI 10.1007/s10811-010-9532-8
Ключевые слова (''Своб.индексиров.''): botryococcus--fatty acid composition--lipid content--salinity--algae--botryococcus--botryococcus braunii
Аннотация: The effect of 0.3 and 0.7 M NaCl on biomass yield, total nitrogen content, intracellular lipid content, and fatty acid profile of the lipids of the alga Botryococcus braunii IPPAS H-252 in different phases of the culture cycle was studied. The presence of sodium chloride in the medium inhibited the growth of algal cells for the first 3 days of the experiment, causing a decrease in total nitrogen, enhanced synthesis of triacylglycerols, and considerable changes in the lipid fatty acid profile: decreases in polyenoic acid contents (from 68.34% to 29.38% and 12.8%) and proportions of long-chain saturated acids (from 0.53% to 5.3% and 14.13% of the total fatty acids) at 0.3 M NaCl and 0.7 M NaCl, respectively. In later phases of the culture, at 0.3 M NaCl, the content of polyenoic acids rose to the values characteristic of the active growth phase of this alga. At 0.7 M NaCl, the proportion of polyenoic acids grew less significantly, but biomass concentration and total nitrogen increased, similarly to the experiment with 0.3 M NaCl. В© 2010 Springer Science+Business Media B.V.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shklavtsova E.S., Ushakova S.A., Shikhov V.N., Anishchenko O.V.
Заглавие : Effects of mineral nutrition conditions on heat tolerance of chufa (Cyperus esculentus L.) plant communities to super optimal air temperatures in the BTLSS
Колич.характеристики :11 с
Коллективы : program of fundamental research for the Russian academies of sciences [56.1.4]
Место публикации : Adv. Space Res.: ELSEVIER SCI LTD, 2014. - Vol. 54, Is. 6. - С. 1135-1145. - ISSN 0273-1177, DOI 10.1016/j.asr.2014.05.031. - ISSN 1879-1948
Примечания : Cited References: 26. - The study was performed within the framework of the program of fundamental research for the Russian academies of sciences for 2013-2020, subject No. 56.1.4.
Предметные рубрики: LIFE-SUPPORT-SYSTEMS
CHLOROPHYLL FLUORESCENCE
STRESS
WASTE
WHEAT
LSS
Ключевые слова (''Своб.индексиров.''): bioregenerative life support system--cyperus esculentus l.--heat shock--mineralized human wastes--chlorophyll fluorescence--lipid peroxidation
Аннотация: The use of mineralized human wastes as a basis for nutrient solutions will increase the degree of material closure of bio-technical human life support systems. As stress tolerance of plants is determined, among other factors, by the conditions under which they have been grown before exposure to a stressor, the purpose of the study is to investigate the level of tolerance of chufa (Cyperus esculentus L.) plant communities grown in solutions based on mineralized human wastes to a damaging air temperature, 45 degrees C. Experiments were performed with 30-day-old chufa plant communities grown hydroponically, on expanded clay aggregate, under artificial light, at 690 mu mol m(-2) s(-1) PAR and at a temperature of 25 degrees C. Plants were grown in Knop's solution and solutions based on human wastes mineralized according to Yu.A. Kudenko's method, which contained nitrogen either as ammonium and urea or as nitrates. The heat shock treatment lasted 20 h at 690 and 1150 mu mol m(-2) s(-1) PAR. Chufa heat tolerance was evaluated based on parameters of CO2 gas exchange, the state of its photosynthetic apparatus (PSA), and intensity of peroxidation of leaf lipids. Chufa plants grown in the solutions based on mineralized human wastes that contained ammonium and urea had lower heat tolerance than plants grown in standard mineral solutions. Heat tolerance of the plants grown in the solutions based on mineralized human wastes that mainly contained nitrate nitrogen was insignificantly different from the heat tolerance of the plants grown in standard mineral solutions. A PAR intensity increase from 690 mu mol m(-2) s(-1) to 1150 mu mol m(-2) s(-1) enhanced heat tolerance of chufa plant communities, irrespective of the conditions of mineral nutrition under which they had been grown. (C) 2014 COSPAR. Published by Elsevier Ltd. All rights reserved.
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