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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T..., Shishatskaya E..., Sevastianov V..., Efremov S..., Mogilnaya O...
Заглавие : Results of biomedical investigations of PHB and PHB/PHV fibers
Колич.характеристики :9 с
Место публикации : Biochem. Eng. J.: ELSEVIER SCIENCE SA, 2003. - Vol. 16, Is. 2. - P125-133. - ISSN 1369-703X, DOI 10.1016/S1369-703X(03)00038-X
Примечания : Cited References: 20
Предметные рубрики: POLYHYDROXYALKANOATES
POLYESTERS
POLYMERS
Ключевые слова (''Своб.индексиров.''): polyhydroxyalkanoates (phas)--poly-3-hydroxybutyrate (phb)--poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (phb/phv)--fibers--implants--biocompatibility--tissue reaction
Аннотация: The paper presents the results of biomedical investigations of sutures made of polyhydroxyalkanoates (PHAs) of two types (poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV)), synthesized by the bacteria Ralstonia eutropha B 5786 in the experiment on test animals in vivo, in comparison with silk and catgut sutures. PHB and PHB/PHV (PHBV) implants produced no adverse effect on physiological, biochemical and functional parameters of the animals during the post-surgery period. The tested PHA sutures featured the necessary strength throughout the healing period of the muscle-fascial cuts. The reaction of tissues to the implantation of PHB and PHB/PHV fibers fitted into the usual scheme characteristic of the wound process and of the reaction to a foreign-body invasion. This reaction and the reaction of tissues to silk had the similar nature and period of inflammation, but it was much less pronounced than the reaction to catgut. The tissue response to the implantation of PHAs consisted in a short-duration (up to 2 weeks) post-traumatic inflammation and the formation of a fibrous capsule less than 200 mum thick during weeks 4-8, which in 4-6 months was reduced to 40-60 mum in the course of reverse development. There were no adverse changes, such as suppurative inflammation, necrosis, calcification, and malignization of the cicatrice, at the site of implantation of PHA filaments, unlike in the cases with silk and catgut. In the case of PHA implantation there was a typical prolonged (throughout the post-surgery monitoring period) pronounced macrophagal stage with a large number of macrophages present. The macrophages were of the phagocytic type and multinucleate giant foreign cells, with a high activity of acid phosphomonoesterase that correlated with the activity of the enzyme in blood. Throughout the period of monitoring no differences in the tissue response to the implantation of the polymer filaments of two PHA types were recorded. (C) 2003 Elsevier Science B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Volova T., Shishatskaya E., Sevastianov V., Efremov S., Mogilnaya O.
Заглавие : Results of biomedical investigations of PHB and PHB/PHV fibers
Место публикации : Biochemical Engineering Journal. - 2003. - Vol. 16, Is. 2. - С. 125-133. - ISSN 1369703X (ISSN) , DOI 10.1016/S1369-703X(03)00038-X
Ключевые слова (''Своб.индексиров.''): biocompatibility--fibers--implants--poly-3-hydroxybutyrate (phb)--poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (phb/phv)--polyhydroxyalkanoates (phas)--tissue reaction--bacteriology--calcification (biochemistry)--copolymers--flammability--tissue--tissue response--biomedical engineering--acid phosphatase--poly(3 hydroxybutyric acid)--poly(3 hydroxybutyric acid) co poly(3 hydroxyvaleric acid)--polyhydroxyalkanoic acid--suture material--unclassified drug--animal experiment--article--bacterial metabolism--biochemistry--biocompatibility--biomedicine--calcification--catgut--correlation analysis--dose time effect relation--enzyme activity--fascia--fiber--foreign body--functional assessment--giant cell--implantation--in vivo study--inflammation--intermethod comparison--macrophage--materials testing--multinuclear cell--muscle injury--necrosis--nonhuman--parameter--phagocytosis--physiology--postoperative period--priority journal--process monitoring--ralstonia eutropha b 5786--scar formation--silk--strength--suture--synthesis--tissue reaction--wautersia eutropha--wound healing--animalia--ralstonia--wautersia eutropha
Аннотация: The paper presents the results of biomedical investigations of sutures made of polyhydroxyalkanoates (PHAs) of two types (poly-3-hydroxybutyrate (PHB) and poly-3-hydroxybutyrate-co-poly-3-hydroxyvalerate (PHB/PHV)), synthesized by the bacteria Ralstonia eutropha B 5786 in the experiment on test animals in vivo, in comparison with silk and catgut sutures. PHB and PHB/PHV (PHBV) implants produced no adverse effect on physiological, biochemical and functional parameters of the animals during the post-surgery period. The tested PHA sutures featured the necessary strength throughout the healing period of the muscle-fascial cuts. The reaction of tissues to the implantation of PHB and PHB/PHV fibers fitted into the usual scheme characteristic of the wound process and of the reaction to a foreign-body invasion. This reaction and the reaction of tissues to silk had the similar nature and period of inflammation, but it was much less pronounced than the reaction to catgut. The tissue response to the implantation of PHAs consisted in a short-duration (up to 2 weeks) post-traumatic inflammation and the formation of a fibrous capsule less than 200 ?m thick during weeks 4-8, which in 4-6 months was reduced to 40-60 ?m in the course of reverse development. There were no adverse changes, such as suppurative inflammation, necrosis, calcification, and malignization of the cicatrice, at the site of implantation of PHA filaments, unlike in the cases with silk and catgut. In the case of PHA implantation there was a typical prolonged (throughout the post-surgery monitoring period) pronounced macrophagal stage with a large number of macrophages present. The macrophages were of the phagocytic type and multinucleate giant foreign cells, with a high activity of acid phosphomonoesterase that correlated with the activity of the enzyme in blood. Throughout the period of monitoring no differences in the tissue response to the implantation of the polymer filaments of two PHA types were recorded. В© 2003 Elsevier Science B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Menzyanova N. G., Pyatina S. А., Nikolaeva E. D., Shabanov A. V., Nemtsev I. V., Stolyarov D. P., Dryganov D. B., Sakhnov E. V., Shishatskaya E. I.
Заглавие : Screening of biopolymeric materials for cardiovascular surgery toxicity—Evaluation of their surface relief with assessment of morphological aspects of monocyte/macrophage polarization in atherosclerosis patients
Место публикации : Toxicol. Rep.: Elsevier Inc., 2019. - Vol. 6. - С. 74-90. - ISSN 22147500 (ISSN) , DOI 10.1016/j.toxrep.2018.11.009
Ключевые слова (''Своб.индексиров.''): atherosclerosis--cell morphology--intravascular stenting--macrophages--monocytes--polyhydroxyalkanoates
Аннотация: The morphotypes of human macrophages (MPh) were studied in the culture on nano-structured biopolymer substrates, made from polyhydroxyalcanoates (PHAs) of five various monomer compositions, followed by the solvent evaporation. Its surface relief, which was further in direct contact with human cells in vitro, was analyzed by atomic force microscopy (AFM) and scanning electron microscopy (SEM). It was shown, that the features of the micro/nano relief depend on the monomeric composition of the polymer substrates. Monocytes (MN) of patients with atherosclerosis and cardiac ischemia, undergoing stenting and conventional anti-atherosclerotic therapy, were harvested prior and after stenting. MN were isolated and cultured, with the transformation into MPh in direct contact with biopolymer culture substrates with different monomer composition and nano-reliefs, and transformed into MPh, in comparison with the same process on standard culture plastic. Sub-populations of cells with characteristic morphology in each phenotypic class were described, and their quantitative ratios for each sample of polymers were counted as an intermediate result in the development of “smart” material for cardiovascular devices. The results obtained allow us to assume, that the processes of MPh differentiation and polarization in vitro depend not only on the features of the micro/nano relief of biopolymer substrates, but also on the initial state of MN in vivo and general response of patients. © 2018 The Authors
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogilnaya O.A., Efremov S.N.
Заглавие : Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures
Колич.характеристики :10 с
Место публикации : J. Mater. Sci.-Mater. Med.: KLUWER ACADEMIC PUBL, 2004. - Vol. 15, Is. 6. - P719-728. - ISSN 0957-4530, DOI 10.1023/B:JMSM.0000030215.49991.0d
Примечания : Cited References: 34
Предметные рубрики: DEGRADATION
POLYESTERS
POLYMERS
FIBERS
PHB
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types-polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV)-featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 mum thick, which became as thin as 40-60 mum after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner. (C) 2004 Kluwer Academic Publishers.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Gordeev S.A., Puzyr A.P.
Заглавие : Degradation of P(3HB) and P(3HB-co-3HV) in biological media
Место публикации : Journal of Biomaterials Science, Polymer Edition. - 2005. - Vol. 16, Is. 5. - С. 643-657. - ISSN 09205063 (ISSN) , DOI 10.1163/1568562053783678
Ключевые слова (''Своб.индексиров.''): degradation rate--fiber properties--morphology--poly(hydroxybutyrate-co-hydroxyvalerate) (p(3hb-co-3hv))--polyhydroxyalkanoates(phas)--polyhydroxybutyrate (p(3hb))--copolymers--degradation--differential scanning calorimetry--enzymes--morphology--scanning electron microscopy--tensile strength--tissue--transmission electron microscopy--degradation rate--fiber properties--polyhydroxyalkanoates (phas)--polyhydroxybutyrate (p(3hb))--biopolymers--buffer--copolymer--poly(3 hydroxybutyric acid)--polyhydroxybutyrate hydroxyvalerate copolymer--unclassified drug--animal experiment--animal model--animal tissue--article--biodegradation--controlled study--crystal structure--fiber--giant cell--in vitro study--in vivo study--macrophage--morphology--nonhuman--ph--priority journal--rat--structure analysis--tensile strength--tissue water--weight reduction--animals--biodegradation, environmental--buffers--humans--hydrogen-ion concentration--hydroxybutyrates--macrophages--microscopy, electron, scanning--microscopy, electron, transmission--muscle, skeletal--polyesters--rats--rats, wistar
Аннотация: The biodegradability of oriented fibers made of polyhydroxybutyrate (P(3HB)) and its co-polymer with ?-hydroxyvalerate (P(3HB-co-3HV)) was investigated in buffer solutions and in biological media in vitro and in vivo. The fibers of both polymer types demonstrated resistance to hydrolytic degradation in buffer solutions at 38В°C and pH from 4.5 to 7.0 (for up to 180 days). It has been found that the biodegradation of the fibers in vitro in blood and serum and in vivo is accompanied by weight losses and minor changes in the microstructure with no significant losses in the tensile strength over a long time (up to 180 days). The biodegradation rate of the less crystalline co-polymer P(3HB-co-3HV) fibers was 1.4-2.0-times higher than that of the homopolymer P(3HB). It has also been shown that the degradation of the fibers in vivo is influenced both by tissue fluid enzymes and cells (macrophages and foreign-body giant cells). The fibers were eroded on the surface only with no gross defects and no dramatic effects on their mechanical performance. В© VSP 2005.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Nikolaeva E.D., Goreva A.V., Brigham C.J., Volova T.G., Sinskey A.J.
Заглавие : An in vivo study of pha matrices of different chemical composition: Tissue reaction and biodegradation
Место публикации : Cellular Transplantation and Tissue Engineering. - 2012. - Vol. 7, Is. 1. - С. 73-80. - ISSN 1815445X (ISSN)
Ключевые слова (''Своб.индексиров.''): degradable polymer matrices--degradation--phas--polyhydroxyalkanoates--subcutaneous implantation--tissue reaction
Аннотация: The study addresses consequences of subcutaneous implantation of film matrices prepared from different PHAs to laboratory animals. No negative effects of subcutaneous implantation of PHA matrices on physiological and biochemical characteristics of the animals were determined. Independently of the matrices composition and duration of the contact with the internal environment of the organism we did not observe any deviations in the behavior of animals, their growth and development, as well as blood functions. Response of the tissues to PHA matrices was comparable with the response to polylactide, but substantially less expressed at the earlier time periods after implantation. Tissues response to implantation of PHA of all types is characterized by short-term (up to 2 weeks) post-traumatic inflammation with formation of fibrous capsules by 30th-60th days with the thickness less than 100 microns, which get thinner down to 40-60 microns by 180th day as the result of involution. No differences in response of tissues and the whole organism were observed for the matrices produced from the homopolymer of 3-hydroxybutyric acid (P3HB), copolymers of 3-hydroxybutyric and 4-hydroxybutyric acids (P3HB/4HB), 3-hydroxybutyric acid and 3-hydroxyvalerianic acids (P3HB/3HV), 3-hydroxybutyric and 3-hydroxyhexanoate acids (P3HB/3HH). Macrophages and foreign-body giant cells actively participate in the response of the tissues to PHAs. In the studied conditions matrices from the copolymers containing 3-hydroxyhexanoate and 4 hydroxybutyrate were determined as more actively degraded PHA. The next less degraded matrices were matrices from the copolymer of P3HB/3HV and the most resistant were P3HB matrices. The slower degradation of PHA matrices was accompanied by delayed development of giantcells response. The studied PHA matrices can be placed in the following range by their degradation: P3HB/3HH - P3HB/4HB - P3HB/HV - P3HB.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Gitelson I.I.
Заглавие : On the involvement of macrophages and phosphomonoesterases in the tissue response to implantation of polyhydroxyalkanoates.
Место публикации : Doklady Biological Sciences. - 2002. - Vol. 383, Is. 1-6. - С. 116-119. - ISSN 00124966 (ISSN)
Ключевые слова (''Своб.индексиров.''): alkane--phosphatase--animal--article--comparative study--foreign body reaction--macrophage--metabolism--physiology--rat--wistar rat--alkanes--animals--foreign-body reaction--macrophages--phosphoric monoester hydrolases--rats--rats, wistar
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Shishatskaya E.I., Volova T.G., Puzyr A.P., Mogilnaya O.A., Efremov S.N.
Заглавие : Tissue response to the implantation of biodegradable polyhydroxyalkanoate sutures
Место публикации : Journal of Materials Science: Materials in Medicine. - 2004. - Vol. 15, Is. 6. - С. 719-728. - ISSN 09574530 (ISSN) , DOI 10.1023/B:JMSM.0000030215.49991.0d
Ключевые слова (''Своб.индексиров.''): necrosis--polymeric implants--suppurative inflammation--tissue reaction--biodegradation--calcification (biochemistry)--cells--copolymers--implants (surgical)--silk--tissue--tumors--materials science--acid phosphatase--copolymer--poly(3 hydroxybutyric acid)--polyhydroxyalkanoic acid--polyhydroxyvaleric acid--unclassified drug--animal experiment--animal model--article--biodegradable implant--blood vessel reactivity--catgut--controlled study--enzyme activity--female--giant cell--histochemistry--inflammation--macrophage--nonhuman--priority journal--rat--silk--suture--tensile strength--tissue reaction--tissue structure--wound healing--young modulus--absorbable implants--animals--female--fibrosis--foreign-body reaction--hydroxybutyrates--muscle, skeletal--polyesters--polymers--rats--rats, wistar--sutures--treatment outcome--wounds, penetrating--animalia
Аннотация: Polyhydroxyalkanoate (PHA) sutures were implanted to test animals intramuscularly, and tissue reaction was investigated and compared with the reaction to silk and catgut. Tested monofilament sutures made of PHAs of two types polyhydroxybutyrate (PHB) and a copolymer of hydroxybutyrate and hydroxyvalerate (PHV) featured the strength necessary for the healing of muscle-fascial wounds. The reaction of tissues to polymeric implants was similar to their reaction to silk and was less pronounced than the reaction to catgut; it was expressed in a transient post-traumatic inflammation (up to four weeks) and the formation of a fibrous capsule less than 200 ?m thick, which became as thin as 4060 ?m after 16 weeks, in the course of reverse development. Macrophages and foreign-body giant cells with a high activity of acid phosphatase were actively involved in this process. PHB and PHB/PHV sutures implanted intramuscularly for an extended period (up to one year) did not cause any acute vascular reaction at the site of implantation or any adverse events, such as suppurative inflammation, necrosis, calcification of the fibrous capsule or malignant tumor formation. No statistically significant differences were revealed in the tissue response to polymer sutures of the two types. Capsules around silk and catgut sutures did not become significantly thinner.
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