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Accumulation of elements by submerged (Stuckenia pectinata (L.) Borner) and emergent (Phragmites australis (Cav.) Trin. ex Steud.) macrophytes under different salinity levels / O. V. Anishchenko, A. P. Tolomeev, E. A. Ivanova [et al.] // Plant Physiol. Biochem. - 2020. - Vol. 154. - P328-340, DOI 10.1016/j.plaphy.2020.05.019 . - ISSN 0981-9428
Кл.слова (ненормированные):
Aquatic plants -- Essential/beneficial elements -- Non-essential elements -- Water conductivity
Аннотация: Accumulation of essential/beneficial and non-essential chemical elements by submerged (Stuckenia pectinata (L.) Borner) and emergent (Phragmites australis (Cav.) Trin. ex Steud.) rooted macrophytes in three water bodies was studied as dependent on water salinity (specific conductivity) and main environmental factors (temperature, pH of water, growing period, element contents in water and sediments). Inductively coupled plasma optical emission spectrometry (ICP - OES) was used for the element detection in the plants, sediments, and water. Multivariate statistics redundancy analysis (RDA) showed that investigated factors together explained 37.8 - 44.7% and 29.9 - 42.6% of the essential/beneficial and non-essential elements variability, respectively, whereas salinity was one of the main factors determining the accumulation of a number of elements (Mg, S, Na, Li, Sr, Sb) by the macrophytes. A decrease in the essential element contents (K, P, N, Cu, Zn) was observed in both species of the macrophytes during the growing season. Environmental factors (salinity, growing period, and pH) were more significant than the element contents in water and the sediments for the essential/beneficial element accumulation by two species of the macrophytes. The element contents in the sediments to a greater extent determined non-essential element accumulation by P. australis than by S. pectinata. The study showed that the efficiency of using P. australis and S. pectinata for remediation of aquatic ecosystems contaminated by heavy metals should not decrease with a salinity increase. © 2020 Elsevier Masson SAS

Scopus
Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok, 50/50, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, 79, Svobodny av., Krasnoyarsk, 660041, Russian Federation

Доп.точки доступа:
Anishchenko, O. V.; Tolomeev, A. P.; Ivanova, E. A.; Drobotov, A. V.; Kolmakova, A. A.; Zuev, I. V.; Gribovskaya, I. V.

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Accumulation of elements by submerged (Stuckenia pectinata (L.) Borner) and emergent (Phragmites australis (Cay.) Trin. ex Steud.) macrophytes under different salinity levels / O. V. Anishchenko, A. P. Tolomeev, E. A. Ivanova [et al.] // Plant Physiol. Biochem. - 2020. - Vol. 154. - P328-340, DOI 10.1016/j.plaphy.2020.05.019. - Cited References:44. - The work was supported by Russian Foundation for Basic Research, grant No. 15-04-00797-a. The authors are grateful to the staff of Analytical Laboratory at the Institute of Biophysics SB RAS for the plant and sediment sample preparation for ICP-OES analysis and Elena Krasova for linguistic check and improvements. . - ISSN 0981-9428

РУБ Plant Sciences
Рубрики:
SEDIMENT QUALITY GUIDELINES
HEAVY-METAL ACCUMULATION
SALT-MARSH
Кл.слова (ненормированные):
Aquatic plants -- Essential/beneficial elements -- Non-essential elements -- Water conductivity
Аннотация: Accumulation of essential/beneficial and non-essential chemical elements by submerged (Stuckenia pectinata (L.) Borner) and emergent (Phragmites australis (Cay.) Trin. ex Steud.) rooted macrophytes in three water bodies was studied as dependent on water salinity (specific conductivity) and main environmental factors (temperature, pH of water, growing period, element contents in water and sediments). Inductively coupled plasma optical emission spectrometry (ICP - OES) was used for the element detection in the plants, sediments, and water. Multivariate statistics redundancy analysis (RDA) showed that investigated factors together explained 37.8 44.7% and 29.9 - 42.6% of the essential/beneficial and non-essential elements variability, respectively, whereas salinity was one of the main factors determining the accumulation of a number of elements (Mg, S, Na, Li, Sr, Sb) by the macrophytes. A decrease in the essential element contents (K, P, N, Cu, Zn) was observed in both species of the macrophytes during the growing season. Environmental factors (salinity, growing period, and pH) were more significant than the element contents in water and the sediments for the essential/beneficial element accumulation by two species of the macrophytes. The element contents in the sediments to a greater extent determined non-essential element accumulation by P. australis than by S. pectinata. The study showed that the efficiency of using P. australis and S. pectinata for remediation of aquatic ecosystems contaminated by heavy metals should not decrease with a salinity increase.

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Держатели документа:
Fed Res Ctr Krasnoyarsk Sci Ctr SB RAS, Inst Biophys SB RAS, Akad Gorodok 50-50, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, 79 Svobodny Av, Krasnoyarsk 660041, Russia.

Доп.точки доступа:
Anishchenko, O., V; Tolomeev, A. P.; Ivanova, E. A.; Drobotov, A., V; Kolmakova, A. A.; Zuev, I., V; Gribovskaya, I., V; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [15-04-00797-a]

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Analytical Enzymatic Reactions in Microfluidic Chips / K. A. Lukyanenko [et al.] // Appl. Biochem. Microbiol. - 2017. - Vol. 53, Is. 7. - P775-780, DOI 10.1134/S0003683817070043. - Cited References:15. - The study was supported by a grant from the Russian Science Foundation (project No. 15-19-10041). . - ISSN 0003-6838. - ISSN 1573-8183

РУБ Biotechnology & Applied Microbiology + Microbiology
Рубрики:
BIOAVAILABLE HEAVY-METALS
   DEVICES
   POINT
   LAB
Кл.слова (ненормированные):
bioluminescence -- luciferase -- microfluidics -- microfluidic chip -- enzymatic -- bioassay
Аннотация: A number of approaches have been proposed and tested to transfer enzymatic reactions into the functional elements of microfluidic chips on the example of the bienzyme bioluminescent reaction involving NAD(P)H:FMN-oxidoreductase and luciferase. Measurement of the catalytic activity of these enzymes (under the influence of pollutants) is the basis of enzymatic bioassay of various liquids. It was found that all of the components of the reaction must be placed in the same cell of the chip to improve the reproducibility of the measurements. The use of starch gel as a carrier for immobilization and gelatin as a scaffold in the reactor of the chip enables the preservation of enzyme activity in the course of sealing the chip at room temperature. It is shown that the components of the reaction should be vigorously stirred in a microfluidic chip reactor to improve the efficiency of the analysis. As a result of the studies, a prototype of microfluidic chip based on the enzymatic bioluminescent reaction is proposed. It is characterized by a detection limit of copper sulfate of 3 mu M that corresponds to the sensitivity of traditional lux-biosensors based on living cells. The analysis time is reduced to 1 min, and the analysis can be performed by individuals without special laboratory skills.

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Держатели документа:
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia.
St Petersburg Inst Fine Mech & Opt, St Petersburg 197101, Russia.
Inst Analyt Instrumentat, St Petersburg 198095, Russia.

Доп.точки доступа:
Lukyanenko, K. A.; Denisov, I. A.; Yakimov, A. S.; Esimbekova, E. N.; Belousov, K. I.; Bukatin, A. S.; Kukhtevich, I. V.; Sorokin, V. V.; Evstrapov, A. A.; Belobrov, P. I.; Russian Science Foundation [15-19-10041]

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Assessment of the Quality of Bottom Sediments in the Middle Reaches of the Yenisei River by Allium test / T. A. Zotina [et al.] // Contemp. Probl. Ecol. - 2019. - Vol. 12, Is. 3. - P265-274, DOI 10.1134/S1995425519030120. - Cited References:35 . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
PLANT ELODEA-CANADENSIS
ANTHROPOGENIC CONTAMINATION
Кл.слова (ненормированные):
ana-telophase -- genotoxicity -- root length -- artificial sediments -- mitotic -- index -- toxicity
Аннотация: The harmful potential of bulk bottom sediments of the Yenisei River has been assessed by the Allium test. Sediment samples have been taken in ten sites on a plot of about 100 km in length in the middle reaches of the Yenisei River affected by urban activity of the city of Krasnoyarsk and industrial plants. The samples of sediments differ considerably in the content of potentially toxic substances (heavy metals, artificial radionuclides, and organic pollutants) and nutrients and in physical properties. We use the mean length of onion root as an indicator of general toxicity, the mitotic index as an indicator of cytotoxicity, and the percentage of anatelophase cells in the apical root meristem with abnormal chromosomes as an indicator of genotoxicity. Both the stimulation and inhibition of indicator endpoints are revealed in sediment samples of the Yenisei River relative to the control (artificial sediments). An increased genotoxicity is recorded in the sample of sediments contaminated by a mixture of organic and chemical toxicants. A significant positive correlation between the percentage of abnormal cells and the content of copper and petrochemicals in the samples is revealed. No extremely strong reactions of onion endpoints to the quality of sediment samples are revealed, which is probably related to relatively low contamination of the sediments studied. This is in agreement with the data of chemical analyses. Based on the testing results, we can conclude that the endpoints of the Allium test are sensitive enough to the quality of bottom sediments of the Yenisei River and can be used for their biotesting.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Fed Res Ctr, Inst Biophys,Krasnoyarsk Sci Ctr, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zotina, T. A.; Trofimova, E. A.; Alexandrova, Yu. V.; Anishchenko, O. V.

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Assessment of the Volatility and Thermal Stability of Chemicals that Stimulate Females of Moina macrocopa (Cladocera) to Produce Diapausing Eggs [Text] / T. S. Lopatina, E. S. Zadereev // Russ. J. Ecol. - 2015. - Vol. 46, Is. 1. - P103-108, DOI 10.1134/S1067413615010105. - Cited References:25 . - ISSN 1067-4136. - ISSN 1608-3334

РУБ Ecology
Рубрики:
SEXUAL REPRODUCTION
   INDUCTION
   DAPHNIA
   METABOLITES
   CRUSTACEA
Кл.слова (ненормированные):
cladocerans -- diapause -- chemical communication -- chemical cues
Аннотация: The volatility and thermal stability of chemicals that are contained in the metabolic products of Moina macrocopa females that stimulate the production of diapausing eggs in these females have been studied in experiments with separately cultivated individuals. It has been shown that the gametogenesis-stimulating chemicals in this species are nonvolatile compounds: the effect of the medium that contains the metabolic products of the organism was not decreased after many hours of blowing with argon. Prolonged heating up to 80 degrees C also did not deactivate the ability of these products to change the reproductive mode. The effect of the medium that contains these products decreased after boiling, but if the possibilities of their oxidation or binding with salts of metals were excluded, they retained the ability to change the reproductive mode of the animals even after boiling.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
ИБФ СО РАН

Доп.точки доступа:
Lopatina, T.S.; Zadereev, E.S.

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Bacteria of Lake Pyasino and Adjacent Rivers after an Accidental Diesel Spill in 2020 / O. V. Kolmakova, M. Y. Trusova, O. A. Baturina, M. R. Kabilov // Contemp. Probl. Ecol. - 2021. - Vol. 14, Is. 4. - P356-367, DOI 10.1134/S1995425521040053. - Cited References:22. - This work was supported by economic agreement no. 223-EP-2020/07 with the Siberian Branch of the Russian Academy of Sciences and the Project Office for the Development of the Arctic (PORA), and State Task of the Fundamental Research Program of the Russian Federation, topic no. 51.1.1. Bioinformatics analysis was supported by basic budget funding from the ICBFM SB RAS (AAAAA17-117020210021-7). . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
SP-NOV.
   GEN. NOV.
   OIL
   BACTERIOPLANKTON
   SEQUENCES
Кл.слова (ненормированные):
bacterioplankton -- sediments -- high-throughput sequencing -- oil spill -- Lake -- Pyasino -- 16S rRNA
Аннотация: The bacterial composition of water and sediments in Lake Pyasino and adjacent rivers has been studied by high-throughput sequencing for the first time. No hydrocarbon-degrading bacteria are found in the river sections exposed to the diesel spillage in May 2020. The Daldykan and Ambarnaya rivers, located downstream of the spill site, are dominated by bacteria that oxidize sulfur and heavy metals. At the same time, these bacteria are not found in Lake Pyasino, which is dominated by small cyanobacteria. Thus, the composition of bacteria in the water and sediments of the Norilsk-Pyasino lake-river system corresponds to the technogenic impact to which it is exposed and also demonstrates the buffer properties of Lake Pyasino as water flows into the Kara Sea.

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Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, Krasnoyarsk 660036, Akademgorodok, Russia.
Siberian Fed Univ, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Siberian Branch, Inst Chem Biol & Fundamental Med, Genom Core Facil, Novosibirsk 630090, Russia.

Доп.точки доступа:
Kolmakova, O. V.; Trusova, M. Yu.; Baturina, O. A.; Kabilov, M. R.; Siberian Branch of the Russian Academy of SciencesRussian Academy of Sciences [223-EP-2020/07]; Project Office for the Development of the Arctic (PORA); State Task of the Fundamental Research Program of the Russian Federation [51.1.1]; ICBFM SB RAS [AAAAA17-117020210021-7]

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Benefit-risk ratio of canned pacific saury (Cololabis saira) intake: Essential fatty acids vs. heavy metals / O. V. Anishchenko [et al.] // Food Chem. Toxicol. - 2017. - Vol. 101. - P8-14, DOI 10.1016/j.fct.2016.12.035 . - ISSN 0278-6915
Кл.слова (ненормированные):
Benefit-risk ratio -- Canned fish -- Heavy metals -- Polyunsaturated fatty acids (PUFA)
Аннотация: Fatty acid (FA) and element contents were studied in 14 brands of canned (in its own juice and with sunflower oil) saury (Cololabis saira), a popular product of Russian market. Canned saury is a valuable source of essential polyunsaturated FA - eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3). To obtain personal daily dose of EPA + DHA of 1 g for prevention of cardiovascular diseases, neural and inflammatory disorders one needs to intake from 26 to 76 g of canned saury, as was calculated for studied brands. ICP-OES analysis of 24 elements showed that Pb concentration in one brand and Cd content in most of studied saury samples exceeded standards for fish meat established by the European Commission. However, values of hazard quotient, HQEFA which estimate benefit-risk ratio of fish intake, indicate that canned saury is safe product for human nutrition. © 2016 Elsevier Ltd

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Держатели документа:
Institute of Biophysics SB RAS, Federal Research Center “Krasnoyarsk Science Center SB RAS”, Akademgorodok, 50/50, Krasnoyarsk, Russian Federation
Siberian Federal University, 79 Svobodny av., Krasnoyarsk, Russian Federation
Federal Regional Center for Standardization, Metrology and Testing in the Krasnoyarsk Region, 1a Vavilov Str., Krasnoyarsk, Russian Federation

Доп.точки доступа:
Anishchenko, O. V.; Sushchik, N. N.; Makhutova, O. N.; Kalachova, G. S.; Gribovskaya, I. V.; Morgun, V. N.; Gladyshev, M. I.

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Benefit-risk ratio of canned pacific saury (Cololabis saira) intake: Essential fatty acids vs. heavy metals / O. V. Anishchenko [et al.] // Food Chem. Toxicol. - 2017. - Vol. 101. - P8-14, DOI 10.1016/j.fct2016.12.035. - Cited References:51. - The work was partly supported by grant NSh-9249.2016.5 from the President of the Russian Federation. . - ISSN 0278-6915. - ISSN 1873-6351

РУБ Food Science & Technology + Toxicology
Рубрики:
EASTERN MEDITERRANEAN SEA
BREAM SPARUS-AURATA
ESSENTIAL ELEMENTS
Кл.слова (ненормированные):
Polyunsaturated fatty acids (PUFA) -- Benefit-risk ratio -- Heavy metals -- Canned fish
Аннотация: Fatty acid (FA) and element contents were studied in 14 brands of canned (in its own juice and with sunflower oil) saury (Cololabis saira), a popular product of Russian market. Canned saury is a valuable source of essential polyunsaturated FA - eicosapentaenoic acid (EPA, 20:5n-3) and docosahexaenoic acid (DHA, 22:6n-3). To obtain personal daily dose of EPA + DHA of 1 g for prevention of cardiovascular diseases, neural and inflammatory disorders one needs to intake from 26 to 76 g of canned saury, as was calculated for studied brands. ICP-OES analysis of 24 elements showed that Pb concentration in one brand and Cd content in most of studied saury samples exceeded standards for fish meat established by the European Commission. However, values of hazard quotient, HQEFA, which estimate benefit -risk ratio of fish intake, indicate that canned saury is safe product for human nutrition. (C) 2016 Elsevier Ltd. All rights reserved.

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Держатели документа:
Krasnoyarsk Sci Ctr SB RAS, Fed Res Ctr, Inst Biophys SB RAS, Akademgorodok 50-50, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, 79 Svobodny Av, Krasnoyarsk 660041, Russia.
Fed Reg Ctr Standardizat Metrol & Testing Krasnoy, 1a Vavilov Str, Krasnoyarsk 660093, Russia.

Доп.точки доступа:
Anishchenko, O. V.; Sushchik, N. N.; Makhutova, O. N.; Kalachova, G. S.; Gribovskaya, I. V.; Morgun, V. N.; Gladyshev, M. I.; Russian Federation [NSh-9249.2016.5]

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Benefit-risk ratio of food fish intake as the source of essential fatty acids vs. heavy metals: A case study of Siberian grayling from the Yenisei River / M. I. Gladyshev [et al.] // Food Chemistry. - 2009. - Vol. 115, Is. 2. - P545-550, DOI 10.1016/j.foodchem.2008.12.062 . - ISSN 0308-8146
Кл.слова (ненормированные):
Benefit-risk ratio -- Essential fatty acids -- Grayling -- Hazard quotient -- Heavy metals
Аннотация: A formula was derived for quantification of benefit-risk ratio (hazard quotient) for the intake of a product containing essential polyunsaturated fatty acids vs. heavy metals. The quotient was used in a three year case study of the contents of essential fatty acids and heavy metals in Siberian grayling, the main food fish from the middle section of the Yenisei River. As found, in general the fish intake was potentially very beneficial for human health, except on a few occasions, when the risk overweighed the benefit because of high contents of chromium in the fish muscle tissue. The data demonstrated the necessity for regular monitoring of the hazard quotients for food fish in wild conditions, based at least on monthly sampling frequency. В© 2008 Elsevier Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Siberian Federal University, Svobodny av. 79, Krasnoyarsk, 660041, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Sushchik, N.N.; Anishchenko, O.V.; Makhutova, O.N.; Kalachova, G.S.; Gribovskaya, I.V.

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10.

Bioaccumulation, inter-organ distribution, and retention of waterborne and dietary Am-241 in silver crucian carp [Text] / T. A. Zotina [et al.] // Toxicol Environ. Chem. - 2014. - Vol. 96, Is. 2. - P243-254, DOI 10.1080/02772248.2014.941368. - Cited References: 25 . - ISSN 0277-2248. - ISSN 1029-0486

РУБ Environmental Sciences + Toxicology
Рубрики:
YENISEI RIVER
   TELEOST FISH
   RADIONUCLIDES
   METALS
   AMERICIUM
   EXPOSURE
   ELEMENTS
   ASSIMILATION
   ELASMOBRANCH
   DEPURATION
Кл.слова (ненормированные):
Americium-241 -- assimilation -- Carassius auratus gibelio -- force-feeding
Аннотация: The bioaccumulation of actinide Am-241 from food and water in bodies of freshwater fish (Carassius auratus gibelio, silver crucian carp) has been investigated in laboratory experiments. Homogenized biomass of submerged macrophytes labeled with Am-241 was injected into the fish gullet. Internal organs and tissues of crucian carp could be ranked according to activity concentration of dietary Am-241 after depuration of the digestive tract as follows: liver > gonads > bones > muscles. Accumulation of waterborne Am-241 in internal organs and tissues of crucian carp mainly occurred via the digestive tract. While the concentration of Am-241 in liver of crucian carps decreased during depuration time, its concentration in bones increased indicating slower transfer of Am-241 to skeleton compared to muscles and liver. The retention of dietary Am-241 in the bodies of crucian carp reached 35%-46% of ingested Am-241: 20%-31% was retained in liver, 0.6%-0.8% in skeleton, and 1.4%-2.0% in muscles. The concentration factor of Am-241 from water was 0.4 for the whole body, 0.3 for liver, 0.01 for muscles, and 0.01 for skeleton. Trace amounts of Am-241 were recorded in viscera and muscles of a wild population of silver crucian carp inhabiting a radioactively contaminated part of the Yenisei River.

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Держатели документа:
[Zotina, T. A.
Trofimova, E. A.
Dementyev, D. V.
Bolsunovsky, A. Y.] Inst Biophys SB RAS, Krasnoyarsk, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Zotina, T.A.; Trofimova, E.A.; Dementyev, D.V.; Bolsunovsky, A.Y.

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11.

Bioluminescent enzyme inhibition-based assay for the prediction of toxicity of pollutants in urban soils / E. M. Kolosova, O. S. Sutormin, L. V. Stepanova [et al.] // Environ. Technol. Innov. - 2021. - Vol. 24. - Ст. 101842, DOI 10.1016/j.eti.2021.101842. - Cited References:46. - This work was supported by the Russian Foundation for Basic Research, the Government of the Krasnoyarsk Region, Russia, and Krasnoyarsk Regional Foundation for Supporting Scientific and Technological Activities, Russia [grant number 18-47-240005] in the field of statistical analysis and interpretation of the data; and the work related to the sample collection was supported by the Ministry of Science and Higher Education of the Russian Federation [grant number FSRZ-2020-0006]. . - ISSN 2352-1864

РУБ Biotechnology & Applied Microbiology + Engineering, Environmental
Рубрики:
FLUORIDE
   BIOASSAYS
   POLLUTION
   METALS
   WATER
Кл.слова (ненормированные):
Urbostratozems -- Soil pollution -- Industrial contamination -- Bioassay -- Bioluminescence
Аннотация: There is a need for rapid simple and informative environmental assessment methods. The present investigation is aimed at assessing the possibility of using the combined enzyme system of luminescent bacteria: NAD(P)H:FMN-oxidoreductase + luciferase (Red + Luc) for predicting the potential toxicity of industrial urbostratozems sampled in the city of Krasnoyarsk. Three groups of urbostratozems polluted with fluorine, arsenic and lead, were tested by the methods of chemical analysis and enzymatic bioassay. Only the assessment of the arsenic-contaminated soil samples showed the dependence between the reduced activity of the enzyme system and the arsenic concentration variations. The results reveal that the sensitivity of the Red + Luc enzyme system to the soil pollutants depends on the properties of the studied soil samples. Moreover, the solubility of lead in the soil samples affects the accuracy of the enzymatic bioassays for soil toxicity testing. The results of the enzymatic bioassay of the fluoride-contaminated soil samples are ambiguous. The obtained data show the relevance of the sample preparation during integral bioassays. In addition, soil properties should be taken into account as well. The current study emphasizes the importance of conducting chemical and biological testing as a combined set to obtain comprehensive information about the anthropogenic load. (C) 2021 Elsevier B.V. All rights reserved.

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Держатели документа:
Siberian Fed Univ, Dept Biophys, 79 Svobodny St, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Krasnoyarsk Agr Res Inst, Fed Res Ctr Krasnoyarsk Sci Ctr Siberian Branch, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Dept Aquat & Terr Ecosyst, Krasnoyarsk 660041, Russia.
Russian Acad Sci, Photobiol Lab, Inst Biophys, Fed Res Ctr `Krasnoyarsk Sci Ctr Siberian Branch, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Kolosova, Elizaveta M.; Sutormin, Oleg S.; Stepanova, L. V.; Shpedt, Aleksandr A.; Rimatskaya, N. V.; Sukovataya, Irina E.; Kratasyuk, Valentina A.; Russian Foundation for Basic Research, the Government of the Krasnoyarsk Region, Russia; Krasnoyarsk Regional Foundation for Supporting Scientific and Technological Activities, Russia [18-47-240005]; Ministry of Science and Higher Education of the Russian Federation [FSRZ-2020-0006]

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12.

Bioluminescent method in studying the complex effect of sewage components / D. I. Stom [et al.] // Archives of Environmental Contamination and Toxicology. - 1992. - Vol. 22, Is. 2. - P203-208 . - ISSN 0090-4341
Кл.слова (ненормированные):
heavy metal -- phenol derivative -- quinone derivative -- article -- bacterium -- bioluminescence -- cell membrane -- nonhuman -- priority journal -- sludge -- ultrastructure -- Benzoquinones -- Catechin -- Hydroquinones -- Luminescent Measurements -- Metals -- Phenol -- Phenols -- Photobacterium -- Sewage -- Vibrio -- Water Pollutants, Chemical -- Bacteria (microorganisms)
Аннотация: The inhibition of bacterial luminescence has been used in testing industrial enterprises sewage. The toxicity of the sewage is less than the total toxicity of separate components due to neutralization of quinone products of polyphenol oxidation in the reactions with the other phenol components of sewage. Toxicity increase is due to their influence on the cell membrane. Studies of cell ultrastructure confirm this fact. The studied mechanism of the complex effect allowed a more accurate forecast of the ecological situation during the discharge of phenol compounds and metals. It also showed the necessity of taking into account the complex effect of sewage components on contaminant discharge into water reservoirs.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Stom, D.I.; Geel, T.A.; Balayan, A.E.; Shachova, G.I.; Kuznetsov, A.M.; Medvedeva, S.E.

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13.

Ca(2+)-activator of the luminescence system of the earthworms Henlea sp., (Annelida: Clitellata: Oligochaeta: Enchytraeidae) / N. S. Rodionova, V. S. Bondar, V. N. Petushkov // Doklady. Biochemistry and biophysics. - 2002. - Vol. 386. - P260-263 . - ISSN 1607-6729
Кл.слова (ненормированные):
calcium -- divalent cation -- edetic acid -- luciferase -- luciferin -- metal -- animal -- annelid worm -- article -- chemistry -- dose response -- enzymology -- genetics -- kinetics -- luminescence -- metabolism -- Animals -- Calcium -- Cations, Divalent -- Dose-Response Relationship, Drug -- Edetic Acid -- Firefly Luciferin -- Kinetics -- Luciferases -- Luminescent Measurements -- Metals -- Oligochaeta

Scopus
Держатели документа:
Institute of Biophysics, Siberian Division, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036 Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Rodionova, N.S.; Bondar, V.S.; Petushkov, V.N.

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14.

Chromosomal abnormalities in roots of aquatic plant Elodea canadensis as a tool for testing genotoxicity of bottom sediments / T. Zotina [et al.] // Ecotoxicol. Environ. Saf. - 2015. - Vol. 122. - P384-391, DOI 10.1016/j.ecoenv.2015.08.021 . - ISSN 0147-6513
Кл.слова (ненормированные):
Anaphase-telophase -- Chromosome damage -- Heavy metals -- Macrophyte -- Radionuclides -- Sediment-contact tests -- Elodea canadensis
Аннотация: Submersed freshwater macrophytes are considered as relevant indicators for use in bulk bottom sediment contact tests. The purpose of this study was to estimate the validity of endpoints of aquatic plant Elodea canadensis for laboratory genotoxicity testing of natural bottom sediments. The inherent level of chromosome abnormalities (on artificial sediments) in roots of E. canadensis under laboratory conditions was lower than the percentage of abnormal cells in bulk sediments from the Yenisei River. The percentage of abnormal cells in roots of E. canadensis was more sensitive to the presence of genotoxic agents in laboratory contact tests than in the natural population of the plant. The spectra of chromosomal abnormalities that occur in roots of E. canadensis under natural conditions in the Yenisei River and in laboratory contact tests on the bulk bottom sediments from the Yenisei River were similar. Hence, chromosome abnormalities in roots of E. canadensis can be used as a relevant and sensitive genotoxicity endpoint in bottom sediment-contact tests. © 2015 Elsevier Inc.

Scopus,
WOS
Держатели документа:
Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Zotina, T.; Medvedeva, M.; Trofimova, E.; Alexandrova, Y.; Dementyev, D.; Bolsunovsky, A.

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15.

Comparative Assessment of the Content of Transition Metals (Cu, Zn, Mn, Pb, and Cd) and Radiocesium (Cs-137) in Pike (Esox lucius) and Burbot (Lota lota) of the Yenisei River / T. A. Zotina, O. V. Anishchenko, E. A. Trofimova, D. V. Dementiev // Contemp. Probl. Ecol. - 2022. - Vol. 15, Is. 1. - P91-99, DOI 10.1134/S1995425522010115. - Cited References:36. - This work was supported by the Russian Foundation for Basic Research, grant no. 18-44-240003, and the Government of Krasnoyarsk krai, together with the Krasnoyarsk Regional Fund for Support of Scientific and Technical Activity, grant no. 20-44-240004. . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
FRESH-WATER FISH
   NORTHERN PIKE
   HEAVY-METALS
   BRAMA L.
   FOOD-WEB
   SIZE
Кл.слова (ненормированные):
piscivorous fish -- toxic metals -- body length -- body weight -- size effect
Аннотация: The trophic position of fish is one of the most important factors controlling the accumulation of potentially toxic elements and compounds in fish tissues, primarily via the spectrum of fish nutrition. In this study, the content of potentially toxic transition metals (Cu, Zn, Mn, Pb, and Cd) and radiocesium (Cs-137) in the edible tissues (muscles and liver) of two representatives of the fish-eating ichthyofauna of the Yenisei River, northern pike (Esox lucius) and burbot (Lota lota), have been comparatively studied relatively to the size of the fish. A significant decrease in the content of radiocesium and zinc in the muscles of pike and zinc in the liver of pike with an increase in body size has been recorded in juvenile pikes with a body weight (W) of less than 0.35 kg. For larger sexually mature pikes, no significant size dependences of the accumulation of metals in tissues are found. A positive correlation is found between the content of Cs-137, Cu, Zn, and Mn in muscles and the size of burbot in the W range from 0.42 to 1.62 kg. The tissues of burbots and pikes of the same size significantly (p < 0.05) differ in the concentration of metals in their tissues: concentrations of Zn and Mn are 1.6-2.2 times higher in the muscle of burbot; the concentration of Pb is 1.8 times higher in muscle of pike; Cu is twice as high in the liver of burbot; and Zn and Mn are 4.7 and 1.6 times higher in the liver of pike, respectively. These differences may be due to the different food spectra of pike and burbot. Concentrations of Cu, Zn, and Mn in the liver of pike are 3-7 times higher than in muscle; the concentration of Cu in the liver of burbot is 5 times higher than in muscle. Pb and Cd tend to be higher in liver than muscle for both fish species. Despite the revealed size dependences, the concentrations of potentially toxic metals and radiocesium in the muscles and liver of fish are below the permissible concentrations for food. These results can be used to assess environmental risks for the population consuming fish, as well as to plan for the long-term environmental monitoring of rivers using representatives of piscivorous fish.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Krasnoyarsk Sci Ctr, Inst Biophys, 50 Akademgorodok, Krasnoyarsk 660036, Russia.
Siberian Fed Univ, Inst Fundamental Biol & Biotechnol, Krasnoyarsk 660036, Russia.

Доп.точки доступа:
Zotina, T. A.; Anishchenko, O., V; Trofimova, E. A.; Dementiev, D., V; Russian Foundation for Basic ResearchRussian Foundation for Basic Research (RFBR) [18-44-240003]; Government of Krasnoyarsk krai; Krasnoyarsk Regional Fund for Support of Scientific and Technical Activity [20-44-240004]

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16.

Concentrations of metals in water of the Yenisei River between Krasnoyarsk and the Angara River outfall in 2010-2015 / D. V. Dementyev [и др.] // Bull. Tomsk Polytech. Univ. Geo Assets Eng. - 2017. - Vol. 328, Is. 3. - С. 54-63 . - ISSN 2500-1019
Кл.слова (ненормированные):
Heavy metals -- Microelements -- Screening -- The Yenisei River -- Water -- Water quality
Аннотация: The relevance of the study is determined by the necessity to obtain the data on concentrations of chemical elements in water of the Yenisei River, which is continuously affected by human activities. The aim of the study is to determine concentrations of such chemical elements as Na, K, Ca, Mg, Zn, Cu, Ba, Al, Mn, U, Mo, Cr, Ni, As, Co, Sr, Fe, Pb, Cd, and Bi in water of the Yenisei River between the city of Krasnoyarsk and the outfall of the Angara River. The methods used in the study. Total contents of the elements were determined by inductively coupled plasma mass spectrometry, using an Agilent 7500a instrument. The results. The study showed the changes in average concentrations of 20 chemical elements in water of the Yenisei River between the city of Krasnoyarsk and the outfall of the Angara River (the village Strelka), for 2010-2015. Comparison of the data obtained with the levels of maximum permissible concentrations showed that the examined part of the Yenisei River contained the increased concentrations of Fe, Cu, Mn, Al, and Zn. For the study period, the magnitudes of average concentrations of Fe reached 1...2 maximum permissible concentrations (MPC) and Cu concentrations reached 1...5 MPC The average concentrations of Mn amounted to 1...3 MPC in the parts of the river over 90 km downstream of Krasnoyarsk (at the villages Atamanovo and Strelka). Cu, Fe, and Zn concentrations tend to increase somewhat in the parts of the river over 90 km downstream of Krasnoyarsk, which may be caused by the influence of the industrial complex of the town Zheleznogorsk and the outfall of the Kan River, with the large industrial facilities located along its banks, in Zelenogorsk, in particular.

Scopus,
WOS
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences FRC KSC SB RAS, 50/50 Akademgorodok, Krasnoyarsk, Russian Federation
ICCT SB RAS FRC KSC SB RAS, 50/24 Akademgorodok, Krasnoyarsk, Russian Federation

Доп.точки доступа:
Dementyev, D. V.; Bolsunovsky, A. Y.; Borisov, R. V.; Alexandrova, Y. V.

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17.

Content of metals in compartments of ecosystem of a Siberian pond / M. I. Gladyshev [et al.] // Archives of Environmental Contamination and Toxicology. - 2001. - Vol. 41, Is. 2. - P157-162, DOI 10.1007/s002440010233 . - ISSN 0090-4341
Кл.слова (ненормированные):
aluminum -- cadmium -- calcium -- chromium -- copper -- heavy metal -- iron -- lead -- magnesium -- manganese -- nickel -- potassium -- sodium -- zinc -- aquatic ecosystem -- biological uptake -- heavy metal -- pond -- article -- bioaccumulation -- ecosystem -- fish -- nonhuman -- pond -- priority journal -- Russian Federation -- sediment -- soil pollution -- water contamination -- Animals -- Ecosystem -- Environmental Monitoring -- Fishes -- Geologic Sediments -- Invertebrates -- Metals, Heavy -- Plants -- Water Pollutants -- Russian Federation
Аннотация: During three field seasons (June-September) of 1997-99 contents of Na, K, Ca, Mg, Fe, Mn, Zn, Cu, Al, Cr, Ni, Cd, and Pb were determined in compartments of ecosystem (surrounding soils, bottom sediments, water, zoobenthos, macrophytes, and fish) of a fish and recreation pond situated at the edge of Krasnoyarsk City (Siberia, Russia). Contents of most parts of metals in soils, water, and macrophytes significantly correlated with each other. As concluded, their contents were determined by natural, general, geochemical peculiarities of the region. Heavy metals, contents of which were higher than federal upper limits of concentration, were revealed. In muscles of fish with different feeding spectra - crucian and perch - concentrations of some metals differed significantly; correlation graphs for metals also had different structures. Comparison of our data with those on diverse aquatic ecosystems of Siberia, Europe, North America, and China published in the last decade was carried out. It was concluded that a distribution of heavy metals in the compartments of an aquatic ecosystem presently have to be determined for each particular water body until general regularities are discovered.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch of Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, 660036, Russian Federation
Krasnoyarsk State Agricultural University, Mira av., 88, Krasnoyarsk, 660049, Russian Federation
Krasnoyarsk State University, Svobodny av., 79, Krasnoyarsk, 660042, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Gribovskaya, I.V.; Moskvicheva, A.V.; Muchkina, E.Y.; Chuprov, S.M.; Ivanova, E.A.

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18.

Content of metals in compartments of ecosystem of a Siberian pond [Text] / M. I. Gladyshev [et al.] // Arch. Environ. Contam. Toxicol. - 2001. - Vol. 41, Is. 2. - P. 157-162. - Cited References: 26 . - ISSN 0090-4341

РУБ Environmental Sciences + Toxicology
Рубрики:
CHIRONOMUS-RIPARIUS MEIGEN
   NELSON RIVER SYSTEM
   AQUATIC MACROPHYTES
   LARVAE DIPTERA
   HEAVY-METAL
   COPPER
   LEAD
   SEDIMENTS
   CADMIUM
   MANITOBA
Аннотация: During three field seasons (June-September) of 1997-99 contents of Na, K, Ca, Mg, Fe, Mn, Zn, Cu, Al, Cr, Ni, Cd, and Pb were determined in compartments of ecosystem (surrounding soils, bottom sediments, water, zoobenthos, macrophytes, and fish) of a fish and recreation pond situated at the edge of Krasnoyarsk City (Siberia, Russia). Contents of most parts of metals in soils, water, and macrophytes significantly correlated with each other. As concluded, their contents were determined by natural, general, geochemical peculiarities of the region. Heavy metals, contents of which were higher than federal upper limits of concentration, were revealed. In muscles of fish with different feeding spectra-crucian and perch-concentrations of some metals differed significantly; correlation graphs for metals also had different structures. Comparison of our data with those on diverse aquatic ecosystems of Siberia, Europe, North America, and China published in the last decade was carried out. It was concluded that a distribution of heavy metals in the compartments of an aquatic ecosystem presently have to be determined for each particular water body until general regularities are discovered.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
Krasnoyarsk State Agr Univ, Krasnoyarsk 660049, Russia
Krasnoyarsk State Univ, Krasnoyarsk 660042, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Gladyshev, M.I.; Gribovskaya, I.V.; Moskvicheva, A.V.; Muchkina, E.Y.; Chuprov, S.M.; Ivanova, E.A.

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19.

Cytogenetic abnormalities in aquatic plant Elodea canadensis in anthropogenic contamination zone of Yenisei River [Text] / M. Y. Medvedeva, A. Y. Bolsunovsky, T. A. Zotina // Contemp. Probl. Ecol. - 2014. - Vol. 7, Is. 4. - P422-432, DOI 10.1134/S1995425514040088. - Cited References: 19. - The authors thank their colleagues from the Radioecology laboratory and Analytical laboratory for gamma spectrometric and chemical analyses. The authors express their appreciation to O.V. Kvitko (Institute of Forest, Siberian Branch, Russian Academy of Sciences, Krasnoyarsk) for her valuable methodical consultations. This research is partially funded by both a RFFI-r_Siberia-a, grant no. 13-04-98004 from the Russian Foundation for Basic Research and project no. 30.5 of the Biological Diversity Program of the Presidium of the Russian Academy of Sciences. . - ISSN 1995-4255. - ISSN 1995-4263

РУБ Ecology
Рубрики:
ALLIUM-TEST
GENOTOXICITY
TOXICITY
Кл.слова (ненормированные):
Elodea canadensis -- bottom sediments -- chromosome abnormalities -- anthropogenic radionuclides -- heavy metals -- genotoxicity
Аннотация: Chromosome abnormalities in ana-telophase cells of apical root meristem of aquatic plant Elodea. canadensis (elodea), sampled in 2011-2012 in the Yenisei River at a site with background level of contamination and at several sites on the stretch contaminated with artificial radionuclides, and with chemical pollutants from municipal and industrial discharges of the Krasnoyarsk city. Lowest rate (5.2%) of cells with chromosome abnormalities was registered at sampling site with background level of contamination upstream of the Krasnoyarsk, highest rate of cells with abnormalities (39.7%)-in roots of elodea sampled in bottom sediments with highest concentration of Cs-137. Sum of rates of cells with abnormalities and rates of cells with all types of abnormalities positively correlated with total concentration of artificial and natural radionuclides, with concentration of artificial radionuclides and Cs-137 in bottom sediments of the Yenisei River (r (2) = 0.91-0.96, p < 0.0005 for sum of rates of cells with abnormalities; r (2) = 0.58-0.92, p < 0.05 for all types of abnormalities).

WOS
Держатели документа:
[Medvedeva, M. Yu
Bolsunovsky, A. Ya
Zotina, T. A.] Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Medvedeva, M.Y.; Bolsunovsky, A.Y.; Zotina, T.A.; RFFI-r_Siberia-a from the Russian Foundation for Basic Research [13-04-98004]; Biological Diversity Program of the Presidium of the Russian Academy of Sciences [30.5]

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20.

Design of bioluminescent biosensors for assessing contamination of complex matrices / E. N. Esimbekova, V. P. Kalyabina, K. V. Kopylova [et al.] // Talanta. - 2021. - Vol. 233. - Ст. 122509, DOI 10.1016/j.talanta.2021.122509. - Cited By :1 . - ISSN 0039-9140
Кл.слова (ненормированные):
Bioluminescent biosensor -- Complex matrices -- Enzyme inhibition-based assay -- Heavy metals -- Pesticides
Аннотация: The presence of potentially toxic xenobiotics in complex matrices has become rather the rule than the exception. Therefore, there is a need for highly sensitive inexpensive techniques for analyzing environmental and food matrices for toxicants. Enzymes are selectively sensitive to various toxic compounds, and, thus, they can be used as the basis for detection of contaminants in complex matrices. There are, however, a number of difficulties associated with the analysis of complex matrices using enzyme assays, including the necessity to take into account properties and effects of the natural components of the test media for accurate interpretation of results. The present study describes the six-stage procedure for designing new enzyme sensors intended for assessing the quality of complex matrices. This procedure should be followed both to achieve the highest possible sensitivity of the biosensor to potentially toxic substances and to minimize the effect of the uncontaminated components of complex mixtures on the activity of the biosensor. The proposed strategy has been tested in designing a bioluminescent biosensor for integrated rapid assessment of the safety of fruits and vegetables. The biosensor is based on the coupled enzyme system NAD(P)H:FMN-oxidoreductase and luciferase as the biorecognition element. The study describes methods and techniques for attaining the desired result in each stage. The proposed six-stage procedure for designing bioluminescent enzyme biosensors can be used to design the enzymatic biosensors based on other enzymes. © 2021 Elsevier B.V.

Scopus
Держатели документа:
Siberian Federal University, 79 Svobodny Prospect, Krasnoyarsk, 660041, Russian Federation
Institute of Biophysics SB RAS, 50/50 Akademgorodok, Krasnoyarsk, 660036, Russian Federation

Доп.точки доступа:
Esimbekova, E. N.; Kalyabina, V. P.; Kopylova, K. V.; Torgashina, I. G.; Kratasyuk, V. A.

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