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1.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakov V.I., Levin L.A., Dubovskaya O.P., Gladyshev M.I.
Заглавие : A circadian rhythm of grazing of microalgae by a laboratory culture of Ceriodaphnia quadrangula
Колич.характеристики :4 с
Место публикации : Biofizika: MEZHDUNARODNAYA KNIGA, 2002. - Vol. 47, Is. 4. - P673-676. - ISSN 0006-3029
Примечания : Cited References: 12
Предметные рубрики: CLOSED CULTIVATORS
FLUORESCENCE
ZOOPLANKTON
Ключевые слова (''Своб.индексиров.''): daily rhythm of grazing--fluorescence--microalgae--flowing-through cultivator--photoperiod--statistical spectral analysis
Аннотация: The circadian rhythm of grazing of microalgae by a laboratory culture of Ceriodaphnia quadrangula under continuous illumination was studied by continuous registration of chlorophyll fluorescence at the outlet of a flow-through cultivator. A culture of green alga Chlorella vulgaris was used as a feed. The data obtained were treated by the statistical spectral analysis. It was found that animals preliminarily grown under a 12 h light : 12 h dark regime and transferred to constant light showed two maxima in the circadian rhythm of grazing with periods of 0.7 and 1.1 h. Animals preliminarily grown under constant light showed no circadian rhythm of grazing. It was concluded that the circadian rhythm of grazing of C quadrangula has endogenous nature and can change according to light conditions.
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2.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakov V.I., Levin L.A., Dubovskaya O.P., Gladyshev M.I.
Заглавие : A circadian rhythm of grazing of microalgae by a laboratory cultureof Ceriodaphnia quadrangula
Место публикации : Biofizika. - 2002. - Vol. 47, Is. 4. - С. 676. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): daily rhythm of grazing--flowing-through cultivator--fluorescence--microalgae--photoperiod--statistical spectral analysis--algae--animalia--ceriodaphnia quadrangula--chlorella vulgaris--chlorophyta
Аннотация: The circadian rhythm of grazing of microalgae by a laboratory culture of Ceriodaphnia quadrangula under continuous illumination was studied by continuous registration of chlorophyll fluorescence at the outlet of a flow-through cultivator. A culture of green alga Chlorella vulgaris was used as a feed. The data obtained were treated by the statistical spectral analysis. It was found that animals preliminarily grown under a 12 h light : 12 h dark regime and transferred to constant light showed two maxima in the circadian rhythm of grazing with periods of 0.7 and 1.1 h. Animals preliminarily grown under constant light showed no circadian rhythm of grazing. It was concluded that the circadian rhythm of grazing of C. quadrangula has endogenous nature and can change according to light conditions.
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3.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Kalacheva G.S., Zhila N.O., Gladyshev M.I., Volova T.G.
Заглавие : A temperature dependence of the intra- and extracellular fatty-acid composition of green algae and cyanobacterium
Колич.характеристики :7 с
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA, 2003. - Vol. 50, Is. 3. - P374-380. - ISSN 1021-4437, DOI 10.1023/A:1023830405898
Примечания : Cited References: 31
Предметные рубрики: SURFACE-FILM
LIPIDS
WATER
BLOOM
SEA
BAY
Ключевые слова (''Своб.индексиров.''): green algae--cyanobacterium--lipids--fatty acids--secretion
Аннотация: The effect of ambient temperature on the composition of intracellular fatty acids and the release of free fatty acids (FFA) into a medium by cyanobacterium Spirulina platensis and eukaryotic microalgae, Chlorella vulgaris and Botryococcus braunii, was studied using their batch cultures. It was found that all the species studied, regardless of their taxonomic status, responded to the temperature regime by similar changes in their intracellular fatty acid composition: the relative content of more unsaturated fatty acids decreased with the elevation of temperature. At the same time, in the prokaryote, this temperature shift blocked, first of all, the elongation of 16:0 to 18:0 and then their further desaturation. In eukaryotes, the change in the desaturation of dienoic to trienoic fatty acids was the most pronounced process. The ratio of dienoic to trienoic fatty acids remained almost unchanged in S. platensis. The relative content of extracellular unsaturated FFA increased in the prokaryotic organism S. platensis at a higher temperature. But no significant changes in the composition of extracellular unsaturated FFA were detected in eukaryotic algae upon temperature elevation.
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4.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Kalacheva G.S., Zhila N.O., Gladyshev M.I., Volova T.G.
Заглавие : A Temperature Dependence of the Intra- and Extracellular Fatty-Acid Composition of Green Algae and Cyanobacterium
Место публикации : Russian Journal of Plant Physiology. - 2003. - Vol. 50, Is. 3. - С. 374-380. - ISSN 10214437 (ISSN) , DOI 10.1023/A:1023830405898
Ключевые слова (''Своб.индексиров.''): cyanobacterium--fatty acids--green algae--lipids--secretion--algae--bacteria (microorganisms)--botryococcus braunii--chlorella vulgaris--chlorophyta--eukaryota--prokaryota--spirulina--spirulina platensis
Аннотация: The effect of ambient temperature on the composition of intracellular fatty acids and the release of free fatty acids (FFA) into a medium by cyanobacterium Spirulina platensis and eukaryotic microalgae, Chlorella vulgaris and Botryococcus braunii, was studied using their batch cultures. It was found that all the species studied, regardless of their taxonomic status, responded to the temperature regime by similar changes in their intracellular fatty acid composition: the relative content of more unsaturated fatty acids decreased with the elevation of temperature. At the same time, in the prokaryote, this temperature shift blocked, first of all, the elongation of 16: 0 to 18:0 and then their further desaturation. In eukaryotes, the change in the desaturation of dienoic to trienoic fatty acids was the most pronounced process. The ratio of dienoic to trienoic fatty acids remained almost unchanged in S. platensis. The relative content of extracellular unsaturated FFA increased in the prokaryotic organism S. platensis at a higher temperature. But no significant changes in the composition of extracellular unsaturated FFA were detected in eukaryotic algae upon temperature elevation.
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5.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zotina T.A., Bolsunovsky A.Y., Bondareva L.G.
Заглавие : Accumulation of Am-241 by suspended matter, diatoms and aquatic weeds of the Yenisei River
Место публикации : J. Environ. Radioact.: ELSEVIER SCI LTD, 2010. - Vol. 101, Is. 2. - С. 148-152. - 5. - ISSN 0265-931X, DOI 10.1016/j.jenvrad.2009.09.009
Примечания : Cited References: 26
Предметные рубрики: RADIONUCLIDES
PLUTONIUM
ELEMENTS
ACTINIDES
PLANTS
Ключевые слова (''Своб.индексиров.''): activity concentration--americium--aquatic plant--asterionella formosa--concentration factor--diatoma vulgare--diatoms--elodea canadensis--seston
Аннотация: In this work we experimentally estimated the capacities of the key components of the Yenisei River (Russia): particulate suspended matter (seston), diatom microalgae, and submerged macrophytes for accumulating Am-241 from water. In our experiments large particles of seston (8 mu m), comparable in size with diatoms, took up most of americium from water. The accumulation of americium by isolated diatom algae (Asterionella formosa and Diatoma vulgare) was lower than by total seston. The concentration factors (CFs) of Am-241 for seston of the Yenisei River in our experiments were (2.8-6.9).10(5); for diatoms -(1.5-4.2)-10(4). The Us for aquatic plant Elodea canadensis were within the same order of magnitude as those for diatoms. Activity concentration and CFs of Am-241 were nearly the same in experiments under dark and light conditions. This is indicative of an energy independent mechanism of americium uptake from the water by diatoms and submerged macrophytes. (C) 2009 Elsevier Ltd. All rights reserved.
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6.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Feio, Maria Joao, Filipe, Ana Filipa, Garcia-Raventos, Aina, Ardura, Alba, Calapez, Ana Raquel, Pujante, Ana Maria, Mortagua, Andreia, Murria, Cesc, Diaz-de-Quijano, Daniel, Martins, Filipa M. S., Duarte, Sofia, Bariain, Marta Sainz, Cordeiro, Rita, Rivera, Sinziana F., Vaisanen, Leif O. S., Fonseca, Amelia, Goncalves, Vitor, Garcia-Vazquez, Eva, Rodriguez, David Vieites, Ivanova, Elena A., Costa, Filipe O., Barquin, Jose, Rojo, Veronica, Vierna, Joaquin, Fais, Maria, Suarez, Marcos, Nieminen, Marko, Hammers-Wirtz, Monica, Kolmakova, Olesia, V, Trusova, Maria Y., Beja, Pedro, Gonzalez, Raquel, Planes, Serge, Almeida, Salome F. P.
Заглавие : Advances in the use of molecular tools in ecological and biodiversity assessment of aquatic ecosystems
Колич.характеристики :22 с
Коллективы : MARE strategic program [UID/MAR/04292/2013]; GeoBioTec strategic program [UID/GEO/04035/2019]; Fundo Regional da Ciencia e Tecnologia (FRCT) [M3.1.a/F/017/2011]; FRESHING Project "Next-generation biomonitoring: freshwater bioassessment and species conservation improved with metagenomics" - Portuguese Foundation for Science and Technology (FCT); COMPETE [PTDC/AAG-MAA/2261/2014 -POCI-01-0145-FEDER-356 016824]; FCTPortuguese Foundation for Science and Technology [SFRH/BD/104703/2014, SFRH/BD/113547/2015, SFRH/BPD/109842/2015]; FCT-PhD programme FLUVIO [PD\BD\52510\2014]; Eurostars project [BIOWAT-KIT_E!11892]; Fundacio Aigues de Barcelona
Место публикации : Limnetica: ASOC ESPAN LIMNOL-MISLATA, 2020. - Vol. 39: 19th Congress of the Iberian-Association-of-Limnology (AIL) (JUN 24-29, 2018, Coimbra, PORTUGAL), Is. 1. - С. 419-440. - ISSN 0213-8409, DOI 10.23818/limn.39.27. - ISSN 1989-1806(eISSN)
Примечания : Cited References:92. - We are grateful to all participants of the special session "The use of molecular tools in ecological and biodiversity assessment of aquatic ecosystems" for the productive discussions during the AIL 2018 meeting (XIX Iberian Association of Limnology meeting in Coimbra (Portugal, June 2018). M.J. Feio is supported by MARE strategic program (UID/MAR/04292/2013); SFP Almeida is supported by GeoBioTec strategic program UID/GEO/04035/2019. R. Cordeiro was supported by a Ph.D. Grant (M3.1.a/F/017/2011) from Fundo Regional da Ciencia e Tecnologia (FRCT); A.F. Filipe and A. Garcia-Raventos were supported by FRESHING Project "Next-generation biomonitoring: freshwater bioassessment and species conservation improved with metagenomics" funded by the Portuguese Foundation for Science and Technology (FCT) and COMPETE (PTDC/AAG-MAA/2261/2014 -POCI-01-0145-FEDER-356 016824); F.M.S. Martins was supported by a FCT PhD grant (SFRH/BD/104703/2014); A.R. Calapez was supported by a grant from the FCT-PhD programme FLUVIO (PD\BD\52510\2014); A.M. Pujante acknowledges the BIOWAT-KIT_E!11892 Eurostars project; Maria Fais and Sofia Duarte were supported, respectively, by a PhD (SFRH/BD/113547/2015) and a post-doc fellowship (SFRH/BPD/109842/2015), from FCT; and C. Murria acknowledges the Fundacio Aigues de Barcelona for funding his research.
Предметные рубрики: BARCODE REFERENCE LIBRARY
METABARCODING APPROACH
RAPID ASSESSMENT
Аннотация: Conservation and sustainable management of aquatic ecosystems is a priority in environmental programs worldwide. However, these aims are highly dependent on the efficiency, accuracy and cost of existent methods for the detection of keystone species and monitoring of biological communities. Rapid advances in eDNA, barcoding and metabarcoding promoted by high-throughput sequencing technologies are generating millions of sequences in a fast way, with a promising cost reduction, and overcoming some difficulties of the traditional taxonomic approaches. This paper provides an updated broad perspective of the current developments in this dynamic field presented in the special session (SS) "The use of molecular tools in ecological and biodiversity assessment of aquatic ecosystems" of the XIX Congress of the Iberian Association of Limnology (AIL2018), held in Coimbra, Portugal. Developments presented are mainly focused on the Iberian Peninsula (Portugal and Spain, including Atlantic Macaronesian islands) but include studies in France, Germany, Finland, Russia (Siberia) and South America. The networks within which these researchers are involved are yet even broader, profiting from existing molecular facilities, and traditional taxonomic expertise, which can be viewed as a characteristic of this new research area. It was evident in the SS that the use of molecular tools is widespread, being used to study a diversity of aquatic systems, from rivers' headwaters to estuaries and coastal lagoons, and volcanic, mountain and frozen lakes to hot springs. The organisms targeted are likewise varied and include fish, macroinvertebrates, meiofauna, microalgae such as diatoms and dinoflagellates, other protists, fungi, and bacteria (cyanobacteria and other). Some studies address the whole biodiversity (i.e., all species present independently of the taxonomic group) from environmental samples of water, biofilms and preservative solution from field samples (e.g., ethanol from macroinvertebrate samples). Great advances were acknowledged in the special session, namely in the use of metabarcoding for detecting hidden biodiversity, juvenile stages, low-abundance species, non-indigenous species and toxicity potential, and ultimately for ecological monitoring of diatoms and invertebrates. Yet, several drawbacks were highlighted and need further work, which include: taxonomic gaps in the reference databases (including gaps at species level and on intraspecific variability) or absence of public databases (e.g. for meiofauna), still high sequencing costs, the need of a substantial bioinformatics effort, difficulties in establishing the amount of environmental sample necessary for a good DNA extraction and the need for testing different genetic markers to obtain accurate results.
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7.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakova A.A., Gladyshev M.I., Kalachova G.S., Kravchuk E.S., Ivanova E.A., Sushchik N.N.
Заглавие : Amino acid composition of epilithic biofilm and benthic animals in a large Siberian river
Место публикации : Freshwater Biology. - 2013. - Vol. 58, Is. 10. - С. 2180-2195. - ISSN 00465070 (ISSN) , DOI 10.1111/fwb.12200
Ключевые слова (''Своб.индексиров.''): amino acids--epilithic microalgae and cyanobacteria--nutritive quality--river ecosystem--zoobenthos
Аннотация: We studied amino acid (AA) composition of epilithic biofilms and zoobenthos near the shore at a middle section of the Yenisei River (Siberia, Russia). We hypothesised that there was an imbalance between the composition and content of amino acids in the biofilm and its consumers, the zoobenthos, as well as between those in the zoobenthos and fish. Based on monthly sampling from 2007 to 2010, there was seasonal variation in AA profiles in the epilithic biofilms, probably caused by the succession of microalgal and cyanobacterial species. Overall, there was an imbalance in the percentage of the essential amino acids (lysine and histidine) between benthic animals and their food (the epilithic biofilm), which suggests that benthic animals may be limited by food quality. Moreover, the zoobenthos had a significantly higher content of AA, relative to carbon, than the biofilm. Based on sampling in 2012, there was an imbalance between the AA profiles of zoobenthos and that of their main consumer, the Siberian grayling (Thymallus arcticus), particularly in the percentages of two essential amino acids, lysine and leucine. In terms of overall content of essential amino acids, the nutritional value to fish of gammarids, which have recently invaded the river, was significantly lower than that of indigenous taxa, trichopteran and chironomid larvae. В© 2013 John Wiley & Sons Ltd.
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8.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakova A. A., Kolmakov, V., I
Заглавие : Amino Acid Composition of Green Microalgae and Diatoms, Cyanobacteria, and Zooplankton (Review)
Колич.характеристики :10 с
Коллективы : State task of the basic research program of the Russian Federation [VI.51.S]
Место публикации : Inland Water Biol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2019. - Vol. 12, Is. 4. - С. 452-461. - ISSN 1995-0829, DOI 10.1134/S1995082919040060. - ISSN 1995-0837(eISSN)
Примечания : Cited References:72. - State task of the basic research program of the Russian Federation, topic number VI.51.S.
Предметные рубрики: BIOCHEMICAL-COMPOSITION
PROTEIN-CONTENT
CRUSTACEAN ZOOPLANKTON
Аннотация: We have reviewed foreign and domestic literature devoted to the study of the amino acid (AA) composition of aquatic organisms representing major groups of producers (green microalgae and diatoms, and cyanobacteria) and primary consumers (zooplankton). Based on published data, we estimate the composition of essential and nonessential AAs of microalgae, cyanobacteria, and zooplankton and determine their differences. It is concluded that the AA composition of major groups of plankton is heterogeneous. The role of AAs as a limiting factor for the development of herbivorous zooplankton is discussed. The prospects and the need for further study of AA composition in order to develop a complete theory of functioning of aquatic ecosystems have been demonstrated.
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9.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakova A. A., Kolmakov V. I.
Заглавие : Amino Acid Composition of Green Microalgae and Diatoms, Cyanobacteria, and Zooplankton (Review)
Место публикации : Inland Water Biol.: Pleiades Publishing, 2019. - Vol. 12, Is. 4. - С. 452-461. - ISSN 19950829 (ISSN) , DOI 10.1134/S1995082919040060
Аннотация: Abstract: We have reviewed foreign and domestic literature devoted to the study of the amino acid (AA) composition of aquatic organisms representing major groups of producers (green microalgae and diatoms, and cyanobacteria) and primary consumers (zooplankton). Based on published data, we estimate the composition of essential and nonessential AAs of microalgae, cyanobacteria, and zooplankton and determine their differences. It is concluded that the AA composition of major groups of plankton is heterogeneous. The role of AAs as a limiting factor for the development of herbivorous zooplankton is discussed. The prospects and the need for further study of AA composition in order to develop a complete theory of functioning of aquatic ecosystems have been demonstrated. © 2019, Pleiades Publishing, Ltd.
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10.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N., Gladyshev M.I., Makhutova O.N., Kalachova G.S., Kravchuk E.S., Ivanova E.A.
Заглавие : Associating particulate essential fatty acids of the omega 3 family with phytoplankton species composition in a Siberian reservoir
Колич.характеристики :14 с
Место публикации : Freshw. Biol.: BLACKWELL PUBLISHING LTD, 2004. - Vol. 49, Is. 9. - P1206-1219. - ISSN 0046-5070, DOI 10.1111/j.1365-2427.2004.01263.x
Примечания : Cited References: 48
Предметные рубрики: ENCLOSED EXPERIMENTAL ECOSYSTEM
FOOD QUALITY
BENTHIC INVERTEBRATES
GREEN-ALGAE
CYANOBACTERIA
DAPHNIA
MICROALGAE
ZOOPLANKTON
LAKE
APHANIZOMENON
Ключевые слова (''Своб.индексиров.''): cyanobacteria--diatoms--fatty acids--food quality--species composition
Аннотация: 1. We studied variation in the composition of fatty acids in the seston of a small freshwater reservoir with changes in phytoplankton composition during four growth seasons. We focused on the dynamics of the omega3 fatty acids because of their potential importance for zooplankton nutrition. 2. Total diatoms were related to the 20:5omega3 fatty acid (eicosapentaenoic, EPA) content in seston. Among two dominant diatom genera, Cyclotella was not associated with EPA content. In contrast, there was a significant correlation between Stephanodiscus and the percentage contribution and content of EPA throughout the study. Hence, freshwater diatoms can differ strongly in content of the essential EPA. 3. We considered abundant cyanobacteria as a potential source of 18:3omega3 fatty acid (linolenic, ALA) to aquatic food webs. Among four dominant cyanobacteria species, two (Anabaena flos-aquae and Planktothrix agardhii) showed significant correlation with the ALA content of the seston, while the other two (Aphanizomenon flos-aquae and Microcystis aeruginosa) did not. 4. Dinophyta had a relatively high level of 22:6omega3 (docosahexaenoic, DHA) for freshwater species and can be also a source of EPA to aquatic food webs. 5. Our results show that various species of diatoms as well as cyanobacteria can be of contrasting nutritional value for zooplankton because of their different content of the essential PUFAs. Diatoms, which are low in EPA, could not be considered as a valuable food, while some field populations of cyanobacteria might be valuable sources of essential ALA.
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11.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakov V.I., Levin L.A., Dubovskaia O.P., Gladyshev M.I.
Заглавие : Circadian rhythms of feeding with microalgae of Ceriodaphnia quadrangula in laboratory culture
Место публикации : Biofizika. - 2002. - Vol. 47, Is. 4. - С. 673-676. - ISSN 00063029 (ISSN)
Ключевые слова (''Своб.индексиров.''): chlorophyll--alga--animal--article--chemistry--circadian rhythm--crustacea--fluorometry--growth, development and aging--photoperiodicity--physiology--algae--animals--chlorophyll--circadian rhythm--crustacea--fluorometry--photoperiod
Аннотация: The circadian rhythm of grazing of microalgae by a laboratory culture of Ceriodaphnia quadrangula under continuous illumination was studied by continuous registration of chlorophyll fluorescence at the outlet of a flow-through cultivator. A culture of green alga Chlorella vulgaris was used as a feed. The data obtained were treated by the statistical spectral analysis. It was found that animals preliminarily grown under a 12 h light: 12 h dark regime and transferred to constant light showed two maxima in the circadian rhythm of grazing with periods of 0.7 and 1.1 h. Animals preliminarily grown under constant light showed no circadian rhythm of grazing. It was concluded that the circadian rhythm of grazing of C. quadrangula has endogenous nature and can change according to light conditions.
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12.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakov V.I., Gaevskii N.A., Ivanova E.A., Dubovskaya O.P., Gribovskaya I.V., Kravchuk E.S.
Заглавие : Comparative analysis of ecophysiological characteristics of Stephanodiscus hantzschii Grun. in the periods of its bloom in recreational water bodies
Колич.характеристики :7 с
Место публикации : Russ. J. Ecol.: MAIK NAUKA/INTERPERIODICA, 2002. - Vol. 33, Is. 2. - P97-103. - ISSN 1067-4136, DOI 10.1023/A:1014448707663
Примечания : Cited References: 31
Предметные рубрики: RESOURCE
Ключевые слова (''Своб.индексиров.''): stephanodiscus hantzschii grun.--chlorophyll a--efficiency in using photosynthetically active radiation
Аннотация: In the periods of summer and autumn bloom of the Stephanodiscus hantzschii Crun. in recreational water bodies, studies on the vertical distribution of chlorophyll a, its contents per unit biomass, efficiency in using photosynthetically active radiation (E-PhAR), and assimilative activity of microalgae were performed. The results confirmed the existence of two ecophysiological forms of St. hantzschii and provided evidence that both forms are typically autotrophic and can efficiently use low-intensity PhAR for photosynthesis.
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13.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakov V.I., Gaevskii N.A., Ivanova E.A., Dubovskaya O.P., Gribovskaya I.V., Kravchuk E.S.
Заглавие : Comparative analysis of ecophysiological characteristics of Stephanodiscus hantzschii Grun. in the periods of its bloom in recreational water bodies
Место публикации : Russian Journal of Ecology. - 2002. - Vol. 33, Is. 2. - С. 97-103. - ISSN 10674136 (ISSN) , DOI 10.1023/A:1014448707663
Ключевые слова (''Своб.индексиров.''): chlorophyll a--efficiency in using photosynthetically active radiation--stephanodiscus hantzschii grun.--algal bloom--comparative study--ecophysiology--photosynthetically active radiation--stephanodiscus hantzschii
Аннотация: In the periods of summer and autumn bloom of the Stephanodiscus hantzschii Crun. in recreational water bodies, studies on the vertical distribution of chlorophyll a, its contents per unit biomass, efficiency in using photosynthetically active radiation (EPhAR), and assimilative activity of microalgae were performed. The results confirmed the existence of two ecophysiological forms of St. hantzschii and provided evidence that both forms are typically autotrophic and can efficiently use low-intensity PhAR for photosynthesis.
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14.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Zadereev E. S., Drobotov A. V., Lopatina T. S., Ovchinnikov S. D., Tolomeev A. P.
Заглавие : Comparison of rapid methods used to determine the concentration, size structure and species composition of algae
Место публикации : J. Sib. Fed. Univ. - Biol.: Siberian Federal University, 2021. - Vol. 14, Is. 1. - С. 5-27. - ISSN 19971389 (ISSN), DOI 10.17516/1997-1389-0338
Аннотация: Traditionally, the abundance, cell size distribution and species identification of algae are determined by microscopic counts. In recent years, various rapid methods have been developed for routine algal studies. However, each of these methods has its drawbacks. It is important for aquatic ecologists to understand the advantages, disadvantages, and limitations of these methods. We compared the sensitivity of three rapid methods (multichannel fluorimeter FluoroProbe, imaging flow cytometer FlowCam, and CASY particle counter) to changes in cell abundance of three algae species (Chlorella vulgaris Beyerinck, Arthrospira platensis Gomont, and Nostoc sp.). We also assessed the ability of rapid methods to estimate the cell abundance of different species in the mixed samples. All instruments showed high sensitivity to changes in the cell abundance of different algae species and a mixture of these species. Any one of these methods, once calibrated, can be reliably used to estimate the abundance of a single-species/laboratory culture of microalgae. At the same time, FlowCam, without preliminary calibration, recorded the cell abundance closest to microscopic counts. When analysing a mixture of three microalgae differing in their cell sizes and spectral characteristics, FluoroProbe showed the highest accuracy in assessing the proportions of species in the mixture and FlowCam - in assessing their abundance. To study mixtures of algae and/or natural phytoplankton communities, it is advisable to use jointly a flow cytometer and a multichannel fluorimeter. The images of algae saved by the flow cytometer, if necessary, can be used to identify them, with a certain accuracy, to the species. Information on cells size and spectral characteristics obtained by two methods will be detailed enough to perform such common tasks as studying trophic interactions between phyto- and zooplankton or creating warning systems to inform of unwanted blooms of phytoplankton and their individual groups (for example, cyanobacteria). © Siberian Federal University. All rights reserved.
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15.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Pisman T.I., Pechurkin N.S., Somova L.A.
Заглавие : Competition between links in "producer-consumer" trophic chains in an aquatic closed system with spatially separated components
Колич.характеристики :5 с
Место публикации : SPACE LIFE SCIENCES: CLOSED ECOLOGICAL SYSTEMS: EARTH AND SPACE APPLICATIONS. Ser. ADVANCES IN SPACE RESEARCH: ELSEVIER SCIENCE BV, 2001. - Vol. 27: F4 4 Symposium of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 9. - P1599-1603. - ISBN 0273-1177, DOI 10.1016/S0273-1177(01)00255-1
Примечания : Cited References: 13
Аннотация: The work analyzes functioning of a "producer-consumer" closed aquatic system with spatially separated links, where each component consisted of two species. Producers in the system were the microalgae of Chlorella vulgaris and Scenedesmus sp., consumers - Paramecium caudatum infusoria and Brachionus sp. rotifers. In the experiment the competing predators were consuming on a mixed culture of algae, and the competition of algae was studied under nitrogen limitation. Under these conditions competitiveness of Scenedesmus was higher than that of Chlorella vulgaris. Metabolism products of Scenedesmus algae have been found to have negative effect on reproduction of Paramecium caudatum protozoa. Predator population dynamics in the "consumer" link demonstrated that the rotifers that consume two algal species are more competitive compared to protozoa feeding on chlorella only. (C) 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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16.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Pisman T.I., Pechurkin N.S., Somova L.A.
Заглавие : Competition between links in "producers-consumer" trophic chains in an aquatic closed system with spatially separated components
Место публикации : Advances in Space Research. - 2001. - Vol. 27, Is. 9. - С. 1599-1603. - ISSN 02731177 (ISSN) , DOI 10.1016/S0273-1177(01)00255-1
Ключевые слова (''Своб.индексиров.''): algae--aquifers--biodiversity--metabolism--nitrogen--protozoa--aquatic closed systems--spatially separated components--space research--aquatic ecosystem--competition--grazing--primary production--fresh water--nitrogen--animal--article--chlorella--food chain--green alga--metabolism--microclimate--paramecium--rotifera--algae, green--animals--chlorella--ecological systems, closed--food chain--fresh water--nitrogen--paramecium--rotifera
Аннотация: The work analyzes functioning of a "producer-consumer" closed aquatic system with spatially separated links, where each component consisted of two species. Producers in the system were the microalgae of Chlorella vulgaris and Scenedesmus sp., consumers - Paramecium caudatum infusoria and Brachionus sp. rotifers. In the experiment the competing predators were consuming on a mixed culture of algae, and the competition of algae was studied under nitrogen limitation. Under these conditions competitiveness of Scenedemus was higher than that of Chlorella vulgaris. Metabolism products of Scenedesmus algae have been found to have negative effect on reproduction of Paramecium caudatum protozoa. Predator population dynamics in the "consumer" link demonstrated that the rotifers that consume two algal species are more competitive compared to protozoa feeding on chlorella only. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.
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17.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gladyshev M.I., Kolmakov V.I., Levin L.A., Dubovskaya O.P.
Заглавие : Daily rhythm of grazing of Ceriodaphnia quadrangula on microalgae by data of continuous fluorometry in flowing cultivator
Колич.характеристики :3 с
Место публикации : Dokl. Akad. Nauk: MEZHDUNARODNAYA KNIGA, 1997. - Vol. 356, Is. 4. - P548-550. - ISSN 0869-5652
Примечания : Cited References: 7
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18.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Sushchik N.N.
Заглавие : Dynamics of algal biomass and composition of extracellular free fatty acids in experimental microecosystems
Место публикации : Biology Bulletin. - 1998. - Vol. 25, Is. 6. - С. 611-616. - ISSN 10623590 (ISSN)
Аннотация: The dynamics of biomass and species composition of microalgae and of composition of extracellular free fatty acids was studied for 98 days in laboratory experimental ecosystems inoculated with water from a forest pond. The microecosystems were kept at two temperature levels: 14-17 and 22-25В°C. During the experiment, a community of planktonic microalgae with the predominance of diatoms was succeeded by a community consisting mostly of green, filamentous algae. The succession of species composition of the algal community was accompanied by changes in the composition of extracellular free fatty acids. Variations of temperature did not markedly affect either the species composition of the microalgal community or the ratio of unsaturated to saturated free fatty acids. В© 1998 MAK Haya/Interperiodica Publishing.
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19.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kolmakov V.I., Anishchenko O.V., Ivanova E.A., Gladyshev M.I., Sushchik N.N.
Заглавие : Estimation of periphytic microalgae gross primary production with DCMU-fluorescence method in Yenisei River (Siberia, Russia)
Место публикации : Journal of Applied Phycology. - 2008. - Vol. 20, Is. 3. - С. 289-297. - ISSN 09218971 (ISSN) , DOI 10.1007/s10811-007-9246-8
Ключевые слова (''Своб.индексиров.''): chlorophyll fluorescence--gross primary production--periphytic microalgae
Аннотация: Periphyton (epilithon) gross primary production (GPP) was estimated using the DCMU-fluorescence method in the Yenisei River. In the unshaded littoral zone, chlorophyll a concentration (Chl a) and GPP value varied from 0.83 to 973.74 mg m-2and 2-304,425 O2 m-2 day -1 (0.64-95 133 mg C m-2 day-1), respectively. Positive significant correlation (r=0.8) between daily GPP and periphyton Chl a was found. Average ratio GPP:Chl a for periphyton was 36.36 mg C mg Chl a m -2 day-1. The obtained GPP values for the Yenisei River have a high significant correlation with values predicted by a conventional empirical model for stream periphyton. We concluded that the DCMU-fluorescence method can be successfully used for measuring of gross primary production of stream phytoperiphyton at least as another useful tool for such studies. В© 2007 Springer Science+Business Media B.V.
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20.

Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gaevskii N.A., Kolmakov V.I., Popel'nitskii V.A., Gold V.M., Dubovskaya O.P.
Заглавие : Evaluation of the effect of light intensity on the measurement of the photosynthetic rate in plankton microalgae by the chlorophyll fluorescence method
Колич.характеристики :6 с
Место публикации : Russ. J. Plant Physiol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2000. - Vol. 47, Is. 6. - P820-825. - ISSN 1021-4437, DOI 10.1023/A:1026671531500
Примечания : Cited References: 21
Предметные рубрики: QUANTUM YIELDS
PHYTOPLANKTON
CHLOROPLASTS
ALGAE
Ключевые слова (''Своб.индексиров.''): fluorescence--chlorophyll--microalgae--photosynthetic rate--photosynthetic activity
Аннотация: The use of relative variable fluorescence (RVF) of chlorophyll, as measured in the presence of Diuron, an inhibitor of electron transfer, for the estimation of the photosynthetic activity of plankton microalgae was analyzed under a wide range of light intensities in the PAR region. Oxygen evolution rates (estimated by the method of light and dark bottles and the amperometric method), RVF, and chlorophyll a concentration were measured in parallel in natural algal cenoses and microecosystems. When the previously used regression equation, in the form A = b(DeltaF/F-d)CchlI, where A is O-2 evolution rate (g/(m(3) h), DeltaF/F-d is RVF (relative units), C-chl is chlorophyll a concentration (mg/m(3)), and I is light intensity (W/m(2)), was verified in the PAR region, we observed a nonlinear dependence of the correction coefficient b on I, which can be described by the formula b = 6.227 x 10(3)rootI. This result agrees with the hypothesis that chlorophyll a fluorescence quenching comprises photochemical (qQ) and energy (qE) components. On the basis of the energy model, we determined the upper limit b(max) = 0.003 for light intensity range I 4.4 W/m(2) and the lower limit b(min)
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