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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Vysotski E.S., Markova S.V., Frank L.A.
Заглавие : Calcium-regulated photoproteins of marine coelenterates
Колич.характеристики :13 с
Место публикации : Mol. Biol.: MAIK NAUKA/INTERPERIODICA/SPRINGER, 2006. - Vol. 40, Is. 3. - С. 355-367. - ISSN 0026-8933, DOI 10.1134/S0026893306030022
Примечания : Cited References: 99
Предметные рубрики: BIOLUMINOMETRIC HYBRIDIZATION ASSAYS
HYDROID OBELIA-GENICULATA
GREEN-FLUORESCENT PROTEIN
POLYMERASE-CHAIN-REACTION
BIOLUMINESCENT IMMUNOASSAY
RECOMBINANT AEQUORIN
CRYSTAL-STRUCTURE
BIOTINYLATED AEQUORIN
ANGSTROM RESOLUTION
CA2+-REGULATED PHOTOPROTEINS
Ключевые слова (''Своб.индексиров.''): bioluminescence--obelin--aequorin--intracellular calcium--molecular diagnosis
Аннотация: Calcium-regulated photoproteins are bioluminescent proteins that are responsible for the luminescence of marine coelenterates. A photoprotein molecule is a stable enzyme-substrate complex consisting of a single polypeptide chain and an oxygen-preactivated substrate, 2-hydroperoxcoelenterazine, which is tightly but noncovalently bound with the protein. Bioluminescence is triggered by Ca2+ and results from decarboxylation of the substrate bound with the protein. This review considers the current information about the structure of photoproteins, the mechanism of the bioluminescent reaction, the function of particular amino acid residues of the active center in catalysis and the formation of the emitter, and the use of photoproteins in bioluminescent microanalysis.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Bashmakova E. E., Kudryavtsev A. N., Frank L. A.
Заглавие : Development of the method to produce functionally active recombinant streptavidin in escherichia coli cells
Место публикации : J. Sib. Fed. Univ. - Biol.: Siberian Federal University, 2020. - Vol. 13, Is. 2. - С. 218-229. - ISSN 19971389 (ISSN), DOI 10.17516/1997-1389-0324
Аннотация: Streptavidin is a homotetrameric protein produced by Streptomyces avidinii, each subunit of which binds biotin (vitamin H), forming a stable complex (Kd = 10-15 M). Streptavidin-biotin coreaction is widely used in analytical systems, for targeted delivery of compounds, for affinity purification, etc. The aim of this study was to develop a rational technique to produce functionally active recombinant streptavidin. Recombinant Escherichia coli strains producing minimal core and full-sized streptavidin variants were obtained. The E. coli BL21 Codon Plus (DE3) RIPL, as host cells, and the pET19b plasmid carrying gene of minimally-sized core (miniSAV) or full-sized (SAV) streptavidin were used. Synthesis of miniSAV results in its localization as insoluble inclusion bodies. Denatured miniSAV yield was 130 mg per liter of E. coli c ulture. T he r enaturation g ives o nly 10- 15 % of the functionally active protein. Full-sized streptavidin localizes in the cytoplasm in a soluble state, but its toxicity causes low yield of the protein (10-13 mg per liter of the culture). The induction of SAV synthesis at the end of the logarithmic stage of cell growth was found to increase the yield of SAV approximately 2-fold. The yield of functionally active protein was 30 mg per liter culture. SAV was produced practically in individual state after affine chromatography on 2-iminobiotin agarose. One molecule of full-sized streptavidin bound 3.9 biotin molecules as was shown by colorimetric analysis using HABA (4-hydroxyazobenzene-2-carboxylic acid). Both streptavidins form sandwichtype complexes with biotinylated molecules in solid-phase microassay conditions. E. coli BL21 Codon Plus (DE3) RIPL/pET19bSAV strain was stable during storage with 20 % glycerol at -70 °C, which was shown by repeated two-year reseeding. The streptavidin producing strain (E. coli BL21 Codon Plus (DE3) RIPL/pET19bSAV) is deposited in the Collection for extremophile microorganisms and type cultures (Institute of Chemical Biology and Fundamental Medicine SB RAS, Novosibirsk), No. 3505. The method for producing functionally active recombinant streptavidin developed in this study ensures its availability for biotechnological research. © Siberian Federal University. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Darin A. V., Babich V. V., Kalugin I. A., Markovich T. I., Rogozin D. Y., Meidus A. V., Darin F. A., Rakshun Y. V., Sorokoletov D. S.
Заглавие : Geochemical Features of Annual Layers of Bottom Sediments of Freshwater Lakes, Studied via Synchrotron Radiation–Induced XRF Microanalysis
Место публикации : Bull. Russ. Acad. Sci. Phys.: Pleiades Publishing, 2019. - Vol. 83, Is. 11. - С. 1437-1440. - ISSN 10628738 (ISSN) , DOI 10.3103/S106287381911008X
Аннотация: Abstract: The possibility of distinguishing layer boundaries through lithological and geochemical indicators (Rb/Sr ratio) is shown by a case study of bottom sediments from Lake Kucherlinskoe (Altai). Based on the data from scanning synchrotron radiation–induced XRF microanalysis and isotope studies, a record of the Tunguska event of 1908 is found in the bottom sediments of Lake Zapovednoye, forty kilometers from the explosion’s epicenter. An anomalous layer in the sediments is associated with an increase in the terrigenous drift in the catchment area due to massive forest outfall and subsequent fires. The possibility of a targeted search for microparticles of extraterrestrial origin in lake sediments is demonstrated. © 2019, Allerton Press, Inc.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Darin, Andrey, Kalugin, Ivan, Maksimova, Natalya, Markovich, Tatiana, Rakshun, Yakov, Sorokoletov, Dmitryi, Darin, Fedor, Ragozin, Denis
Заглавие : Lake Shira Level Changes in Late Holocene
Колич.характеристики :2 с
Коллективы : RFBR [13-05-00871, 14-02-00631]
Место публикации : Acta Geol. Sin.-Engl. Ed.: WILEY-BLACKWELL, 2014. - Vol. 88. - С. 3-4. - ISSN 1000-9515, DOI 10.1111/1755-6724.12265_1. - ISSN 1755-6724(eISSN)
Примечания : Cited References:4. - This work was performed on equipment of the Common Use Center at theSiberian Synchrotron and Teraherz Radiation Center (Novosibirsk). It wassupported by the RFBR projects 13-05-00871, 14-02-00631.
Ключевые слова (''Своб.индексиров.''): lake shira--varve--synchrotron radiation--scanning x_ray fluorescence--microanalysis
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Babich, Valery V., Darin, Andrey, V, Kalugin, Ivan A., Markovich, Tatyana, I, Zykov, Viktor V., Rogozin, Denis Yu
Заглавие : SEASONAL VARIABILITY OF SEDIMENTATION FLOWS IN SALT MEROMICTIC LAKE SHIRA (KHAKASSIA)
Колич.характеристики :13 с
Коллективы : Ministry of Science and Higher Education of the Russian Federation; RFBRRussian Foundation for Basic Research (RFBR) [21-54-52001, 19-05-50046]
Место публикации : Bull. Tomsk Polytech. Univ.-Geo Assets Eng.: TOMSK POLYTECHNIC UNIV, PUBLISHING HOUSE, 2021. - Vol. 332, Is. 12. - С. 22-34. - ISSN 2500-1019, DOI 10.18799/24131830/2021/12/3178. - ISSN 2413-1830(eISSN)
Примечания : Cited References:29. - The work was carried out on state assignment of IGM SB RAS, supported by the Ministry of Science and Higher Education of the Russian Federation, with partial support of the RFBR - grant 21-54-52001 (setting of traps, sampling) and grant 19-05-50046 (micro-XRF-SR).
Предметные рубрики: CENTRAL TIBETAN PLATEAU
NAM-CO
VARVE
Аннотация: The relevance of the work is caused by the need to study seasonal variations in the volume and composition of sedimentation flows in modern lakes and their relationship with weather and climatic factors, which can serve as a basis for reconstructing climatic changes in the past. The main aim: to assess seasonal changes in the mass, velocity and chemical composition of sedimentary material entering Lake Shira on the basis of generalization and analysis of the results of multiyear annual monitoring of the material of sedimentation traps incubated in the lake. Object of the study was the drainless, slightly saline meromictic lake Shira, located in the steppe intermontane depression on the territory of the Republic of Khakassia. Methods: long-term seasonal monitoring of sedimentary material forming bottom sediments using sedimentation traps incubated in the lake; study of the obtained material for a wide range of parameters (biological, hydrochemical, lithological-geochemical, granulometric, etc.) by various conventional methods, including the method of X-ray fluorescence microanalysis on synchrotron radiation beams (XRF-SI), adapted for the study of bottom samples; computer statistical analysis of the data obtained (multiple regression method, cross-correlation analysis, etc.) in order to identify the relationship between regional temperatures and the chemical composition of the deposited material with the construction of a regression model. Results. Based on the study of the amount and elemental composition of the sedimentary material of the seasonal bottom traps of Lake Shira, collected for 2012-2017, a dynamic model of the seasonal influx of sedimentary flows in lakes of this landscape-geochemical type was formulated. It has been established that the most intensive sedimentation of the material occurs in the summer-autumn period, less - in the winter-spring period. At the same time, in spring, the accumulation of allochthonous (terrigenous) aleurite material, supplied with flood waters, predominates; in the summer-autumn period, simultaneously with the deposition of clastogenic pelitic material, biogenic and chemogenic materials are accumulated in sediments in large quantities. In winter, clastogenic and biogenic processes of sedimentation practically stop, only chemogenic sedimentation of carbonates is observed. It was established that the sedimentation of allochthonous material entering the reservoir occurs during two-three months, which indicates a certain inertness of sedimentation. The presence of a stable relationship between the mass and chemical composition of terrigenous material entering the lake with the regional temperature of the near-surface air, which is one of the main regulators of the water balance of the reservoir, is shown.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Krasitskaya V. V., Davydova A. S., Vorobjeva M. A., Frank L. A.
Заглавие : The Ca2+-Regulated Photoprotein Obelin as a Target for the RNA Aptamer Selection
Место публикации : Russ. J. Bioorg. Chem.: Pleiades Publishing, 2018. - Vol. 44, Is. 3. - С. 296-301. - ISSN 10681620 (ISSN) , DOI 10.1134/S1068162018030093
Ключевые слова (''Своб.индексиров.''): 2'-fluoro-rna--in vitro selection--rna aptamers--са2+-regulated photoprotein obelin
Аннотация: A variant of the Ca2+-regulated photoprotein obelin elongated with a hexahistidine peptide from the N-terminus was developed and studied. After immobilization on a metal-affine sorbent, the hybrid protein was applied as a target for the in vitro selection of RNA aptamers. According to the data of bioluminescent solid-phase microanalysis, the selection was shown to enrich the RNA library with obelin-affine molecules. © 2018, Pleiades Publishing, Ltd.
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