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ACCUMULATION OF POLYHYDROXYBUTYRATE BY THE AEROBIC CO-OXIDIZING BACTERIUM SELIBERIA-CARBOXYDOHYDROGENA [Text] / T. G. VOLOVA, G. S. KALACHEVA, V. M. KONSTANTINOVA // Microbiology. - 1994. - Vol. 63, Is. 2. - P109-112. - Cited References: 20 . - 4. - ISSN 0026-2617

РУБ Microbiology

Аннотация: The synthesis of polyhydroxybutyrate by the carboxydobacterium Seliberia carboxydohydrogena under various growth conditions was studied. The bacterium was able to accumulate poly-beta-hydroxybutyric acid and a heteropolymer that was a product of copolymerization of hydroxybutyric and hydroxyvaleric acids. Nitrogen and sulfur exerted the strongest effect on polymer accumulation in carboxydobacteria: The maximum polymer concentrations in cells reached 28%. Specimens of polyhydroxybutyrate films were obtained, and their degradability by soil microflora was shown.
: 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
VOLOVA, T.G.; KALACHEVA, G.S.; KONSTANTINOVA, V.M.

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LUMINESCENT BACTERIAL SYMBIONTS AND COMMENSALS OF LUMINESCENT AND NONLUMINESCENT MARINE ANIMALS OF THE INDIAN-OCEAN [Text] / G. A. VYDRYAKOVA [et al.] // Microbiology. - 1995. - Vol. 64, Is. 5. - P. 589-592. - Cited References: 18 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
BIOLUMINESCENCE
SEAWATER
Аннотация: Approximately 100 fish belonging to 24 families and several representatives of cephalopods, prawns, and euphausiids were investigated for the presence of luminescent bacteria. Species identification of isolated luminescent bacteria was performed, and the frequency and ratio of their occurrence in the gastrointestinal microflora of marine animals were determined. Luminescent bacteria occurred in 23 - 65% of the fish, depending on the habitat depth, and their ratio varied from 8 to 60% of the total gastrointestinal microflora of fish. The free-living luminescent bacteria were found in 50% of the seawater samples from depths down to 1000 m. The luminescent bacterium Photobacterium phosphoreum was dominant among the isolated cultures.

WOS : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
VYDRYAKOVA, G.A.; KUZNETSOV, A.M.; PRIMAKOVA, G.A.; CHUGAEVA, Y.V.; FISH, A.M.

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Micro- and macroorganisms in life support systems (problem of coexistence) / L. A. Somova, N. S. Pechurkin, R. C. Huttenbach // Advances in Space Research. - 1996. - Vol. 18, Is. 12. - P259-263, DOI 10.1016/0273-1177(96)00120-2 . - ISSN 0273-1177
Аннотация: Every life support system has an associated microflora that is not essential to functioning of the system. At the same time, the confined space of a closed system increases the significance of the associated microflora causing closer contact between components and enhancing the intensity of exchange between them. For any life support system that is functioning normally, there exists an optimum between the effort necessary to maintain the system in a healthy state and the damage the introduction of alien microflora can cause. Copyright В© 1996 COSPAR.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk, 660036, Russian Federation
Nelson Space Services Ltd., 90 London Road, London SEI 6LN, United Kingdom : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.; Huttenbach, R.C.

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Catalase activity as a potential indicator of the reducer component of small closed ecosystems / A. B. Sarangova, L. A. Somova, T. I. Pisman // Advances in Space Research. - 1997. - Vol. 20, Is. 10. - P1945-1948 . - ISSN 0273-1177
Кл.слова (ненормированные):
carboxymethylcellulose -- catalase -- animal -- article -- Bacillus -- bacterial count -- Chlorella -- culture medium -- enzymology -- growth, development and aging -- metabolism -- microclimate -- Paramecium -- Animals -- Bacillus -- Carboxymethylcellulose -- Catalase -- Chlorella -- Colony Count, Microbial -- Culture Media -- Ecological Systems, Closed -- Paramecium
Аннотация: Dynamics of catalase activity has been shown to reflect the growth curve of microorganisms in batch cultivation (celluloselythic bacteria Bacillus acidocaldarius and bacteria of the associated microflora Chlorella vulgaris). Gas and substrate closure of the three component ecosystems with spatially separated components "producer-consumer-reducer" (Chl. vulgaris-Paramecium caudatum-B. acidocaldarius, two bacterial strains isolated from the associated microflora Chl. vulgaris) demonstrated that the functioning of the reducer component can be estimated by the catalase activity of microorganisms of this component. В© 1997 COSPAR. Published by Elsevier Science Ltd.

Scopus
Держатели документа:
Institute of Biophysics, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sarangova, A.B.; Somova, L.A.; Pisman, T.I.

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Catalase activity as a potential indicator of the reducer component of small closed ecosystems [Text] / A. B. Sarangova, L. A. Somova, T. I. Pisman ; ed. RM Wheeler [et al.] // LIFE SCIENCES: LIFE SUPPORT SYSTEMS STUDIES-I. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON PRESS LTD, 1997. - Vol. 20: F4.6, F4.8, F4.2 and F4.9 Symposia of COSPAR Scientific Commission F on Life Sciences - Life Support System Studies-I, at the 31st COSPAR Scientific Assembly (JUL 14-SEP 21, 1996, BIRMINGHAM, ENGLAND), Is. 10. - P. 1945-1948, DOI 10.1016/S0273-1177(97)00630-3. - Cited References: 8 . - ISBN 0273-1177. - ISBN 0-08-043307-3

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: Dynamics of catalase activity has been shown to reflect the growth curve of microorganisms in batch cultivation (celluloselythic bacteria Bacillus acidocaldarius and bacteria of the associated microflora Chlorella vulgaris). Gas and substrate closure of the three component ecosystems with spatially separated components "producer-consumer-reducer" (Chl, vulgaris-Paramecium caudatum-B. acidocaldarius, two bacterial strains isolated from the associated microflora Chl. vulgaris) demonstrated that the functioning of the reducer component can be estimated by the catalase activity of microorganisms of this component. (C) 1997 COSPAR. Published by Elsevier Science Ltd.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Sarangova, A.B.; Somova, L.A.; Pisman, T.I.; Wheeler, RM \ed.\; Garland, JL \ed.\; Tibbitts, TW \ed.\; Nielsen, SS \ed.\

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Experimental microcosms as models of natural ecosystems for monitoring survival of genetically modified microorganism. / U - Popova LYu [et al.] // Life support & biosphere science : international journal of earth space. - 1999. - Vol. 6, Is. 3. - P193-197 . - ISSN 1069-9422
Кл.слова (ненормированные):
bacterial DNA -- recombinant DNA -- adaptation -- article -- ecosystem -- Escherichia coli -- genetics -- microbiology -- plasmid -- risk assessment -- Adaptation, Biological -- DNA, Bacterial -- DNA, Recombinant -- Ecosystem -- Escherichia coli -- Microbiology -- Plasmids -- Risk Assessment -- Soil Microbiology -- Water Microbiology
Аннотация: An experimental approach for investigation of genetically modified microorganisms (GMMO) introduced into model ecosystems to evaluate potential risk of propagation of recombinant plasmids in surrounding medium has been developed. The object of modeling was Escherichia coli Z905 strain with a recombinant plasmid with bacterial luminescence genes, which was introduced into water microcosms of different structure. The approach involves comprehensive investigation of GMMO at four hierarchical levels: molecular (retaining the structure of the plasmid and expression of cloned genes); cellular (variation of metabolic activity); population (competitive power and metabolic interactions of GMMO with indigenous microflora, migration of recombinant and natural plasmids); ecosystem (effect of GMMO and cloned genes on ecosystem parameters). The experimental evidence and theoretical estimates are intended to form grounds to develop a basic version of an ecological certificate for different GMMO variants.

Scopus
Держатели документа:
Institute of Biophysics (Russian Academy of Sciences, Siberian Branch), Krasnoyarsk, Russia. : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
, U - Popova LYu; Pechurkin, N.S.; Maksimova, E.E.; Kargatova, T.V.; , U - Krylova TYu; Lobova, T.I.; Boyandin, A.N.

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Comparative characterization of the growth of recombinant Escherichia coli strains and species of the aboriginal microflora of aquatic microecosystems under selective and nonselective conditions [Текст] / T. V. Kargatova [и др.] // Izv. Akad. Nauk Ser. Biol. - 1999. - Is. 2. - P. 152-157. - Cited References: 9 . - ISSN 0002-3329

РУБ Biology
Рубрики:
GENETICALLY-MODIFIED MICROORGANISMS
Аннотация: We have studied the kinetic characteristics of several isolates of Escherichia coli Z905 recombinant strain after introduction of the strain into model aquatic ecosystems. Most E. coli Z905 isolates grown as batch cultures under selective conditions (0.5 mu g/ml ampicillin) showed better kinetic characteristics of growth than did related species of native microflora, which originally populated laboratory microcosms.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Maksimova, E.E.; Krylova, T.Y.; Popova, L.Y.

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Functional, regulatory and indicator features of microorganisms in man-made ecosystems / L. A. Somova, N. S. Pechurkin // Advances in Space Research. - 2001. - Vol. 27, Is. 9. - P1563-1570, DOI 10.1016/S0273-1177(01)00247-2 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biomass -- Carbon dioxide -- Ecosystems -- Life support systems (spacecraft) -- Photosynthesis -- Sewage treatment -- Soils -- Human microfloras -- Microorganisms -- carbon -- carbon dioxide -- artificial ecosystem -- article -- biomass -- bioreactor -- ecosystem -- human -- intestine -- metabolism -- microbiology -- microclimate -- plant root -- sewage -- wheat -- Biomass -- Bioreactors -- Carbon -- Carbon Dioxide -- Ecological Systems, Closed -- Ecosystem -- Environmental Microbiology -- Humans -- Intestines -- Life Support Systems -- Plant Roots -- Sewage -- Triticum -- Waste Disposal, Fluid
Аннотация: Functional, regulatory and indicator features of microorganisms in development and functioning of the systems and sustaining stability of three man-made ecosystem types has been studied. 1) The functional (metabolic) feature was studied in aquatic ecosystems of biological treatment of sewage waters for the reducer component. 2) The regulatory feature of bacteria for plants (producer component) was studied in simple terrestrial systems "wheat plants-rhizospheric microorganisms - artificial soil" where the behavior of the system varied with activity of the microbial component. For example with atmospheric carbon dioxide content elevated microbes promote intensification of photosynthesis processes, without binding the carbon in the plant biomass. 3) The indicator feature for the humans (consumer component) was studied in Life Support Systems (LSS). High sensitivity of human microflora to system conditions allowed its use as an indicator of the state of both system components and the entire ecosystem. В© 2001 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Siberian Branch, Russian Academy of Sciences, Akademgorodok, Krasnoyarsk, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Somova, L.A.; Pechurkin, N.S.

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Formation of higher plant component microbial community in closed ecological system [Text] / L. S. Tirranen // Acta Astronaut. - 2001. - Vol. 49, Is. 1. - P. 47-52, DOI 10.1016/S0094-5765(01)00005-4. - Cited References: 13 . - ISSN 0094-5765

РУБ Engineering, Aerospace

Аннотация: Closed ecological systems (CES) place at the disposal of a researcher unique possibilities to study the role of microbial communities in individual components and of the entire system. The microbial community of the higher plant component has been found to form depending on specific conditions of the closed ecosystem: length of time the solution is reused, introduction of intrasystem waste water into the nutrient medium, effect of other component of the system, and system closure in terms of gas exchange. The higher plant component formed its own microbial complex different from that formed prior to closure. The microbial complex of vegetable polyculture is more diverse and stable than the monoculture of wheat. The composition of the components' microflora changed, species diversity decreased, individual species of bacteria and fungi whose numbers were not so great before the closure prevailed. Special attention should be paid to phytopathogenic and conditionally pathogenic species of microorganisms potentially hazardous to man or plants and the least controlled in CES. This situation can endanger creation of CES and make conjectural existence of preplanned components, man, specifically, and consequently, of CES as it is. (C) 2001 International Astronautical Federation. Published by Elsevier Science Ltd.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tirranen, L.S.

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10.

Coexistence of genetically engineered Escherichia coli strains and natural microorganisms in experimental aquatic microcosms [Text] / T. V. Kargatova, E. E. Maksimova, L. Y. Popova // Microbiology. - 2001. - Vol. 70, Is. 2. - P. 211-216, DOI 10.1023/A:1010437731920. - Cited References: 17 . - ISSN 0026-2617

РУБ Microbiology
Рубрики:
SURVIVAL
BACTERIA
GROWTH
Кл.слова (ненормированные):
Escherichia coli -- aquatic microecosystems -- recombinant plasmid
Аннотация: In experimental aquatic microcosms (AMCs), the population of the Escherichia coli strain Z905 harboring the recombinant plasmid pPHL7 (Ap(r)Lux(+)) was found to gradually accumulate AMC-adapted cells, which retained the plasmid but differed from the original cells in some biochemical and physiological characteristics. Both the original and the AMC-adapted E. coil cells could coexist with the native AMC microflora for one year or longer. When introduced into AMCs together with native pseudomonads, the AMC-adapted E. coil Z905-33 (pPHL7) cells were more competitive than the nonadapted cells.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Div, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Maksimova, E.E.; Popova, L.Y.

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11.

Antibiotic resistance of heterotrophic bacteria in Shira lake: Natural and anthropogenic impacts / T. I. Lobova, Yu. V. Barkhatov, L. Yu. Popova // Aquatic Microbial Ecology. - 2002. - Vol. 30, Is. 1. - P11-18 . - ISSN 0948-3055
Кл.слова (ненормированные):
Anthropogenic load -- Antibiotic resistance -- Brackish lake -- Halotolerance -- Heterotrophic bacteria -- Mathematical modeling -- algae -- Bacteria (microorganisms) -- Chlorophyta
Аннотация: Studies were conducted to investigate the antibiotic resistance of freshwater, halophile and moderate halotolerant bacteria isolated from the nearshore part of Shira lake, which is affected by the activity of a health resort, and from the central part of the lake in the summer (June to August) of 1999. It has been shown that the allochthonous microflora, which is brought into the lake with the resort effluent in mid-summer, is the anthropogenic factor contributing to an increase in the number of freshwater bacteria that feature multiple antibiotic resistance in the central part of the lake. It has been found that resistance to ampicillin of freshwater and halophile heterotrophic bacteria is related to the increase in the biomass of blue-green and green algae in the central part of Shira lake between mid-July and the end of August. A mathematical model has been constructed to describe the dynamics of the antibiotic resistance of heterotrophic bacteria in the close-to-resort and the central parts of Shira lake under natural and anthropogenic impacts.

Scopus
Держатели документа:
Centre Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Academgorodok 50, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lobova, T.I.; Barkhatov, Yu.V.; Popova, L.Yu.

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12.

Growth of dominant zooplankton species feeding on plankton microflora in Lake Shira / T. A. Temerova, A. P. Tolomeyev, A. G. Degermendzhy // Aquatic Ecology. - 2002. - Vol. 36, Is. 2. - P235-243, DOI 10.1023/A:1015607304508 . - ISSN 1386-2588
Кл.слова (ненормированные):
Arctodiaptomus salinus -- Brachionus plicatilis -- Life history -- Reproduction -- Specific growth rate -- diet -- food limitation -- growth rate -- life history -- reproduction -- saline lake -- zooplankton -- Russian Federation -- algae -- Arctodiaptomus -- Bacteria (microorganisms) -- Brachionus -- Brachionus plicatilis -- Calanoida -- Copepoda -- Cyanobacteria -- Invertebrata -- Rotifera
Аннотация: Batch cultures and continuous flow cultures were used to study the growth rates of zooplankton species from Shira lake, the rotifer Brachionus plicatilis Muller and calanoid copepod Arctodiaptomus salinus Daday, which were fed on phytoplankton and bacterioplankton from the lake. Analyses of the birth and survival rates were used to demonstrate that the lake phytoplankton, consisting mostly of cyanobacteria and diatomaceous algae, is inadequotes for optimal realisation of the reproductive potential of B. plicatilis when compared with the bacterial diet. The study revealed that the kinetic growth characteristics of the two zooplankters were similar: B. plicatilis rmax, 0.120 d-1; S0, 0.253; and Ks, 0.114 mg dry mass l-1; and for A. salinus rmax, 0.129 d-1; S0, 0.240; and Ks, 0.171 mg dry mass l-1. Fluctuations in natural food concentration reduced the growth rate of both species. Even though the threshold concentration of food for B. plicatilis and A. salinus were quite similar, the copepods were less sensitive to food limitation.

Scopus
Держатели документа:
Institute of Biophysics of SB RAS, Krasnoyarsk 660036, Russian Federation : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Temerova, T.A.; Tolomeyev, A.P.; Degermendzhy, A.G.

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13.

Antibiotic resistance of heterotrophic bacteria in Shira lake: natural and anthropogenic impacts [Text] / T. I. Lobova, Y. V. Barkhatov, L. Y. Popova // Aquat. Microb. Ecol. - 2002. - Vol. 30, Is. 1. - P. 11-18, DOI 10.3354/ame030011. - Cited References: 33 . - ISSN 0948-3055

РУБ Ecology + Marine & Freshwater Biology + Microbiology
Рубрики:
MARINE-BACTERIA
   DIVERSITY
   POLLUTION
   COMMUNITY
   PATTERNS
   PLANTS
Кл.слова (ненормированные):
heterotrophic bacteria -- halotolerance -- antibiotic resistance -- anthropogenic load -- brackish lake -- mathematical modeling
Аннотация: Studies were conducted to investigate the antibiotic resistance of freshwater, halophile and moderate halotolerant bacteria isolated from the nearshore part of Shira lake, which is affected by the activity of a health resort, and from the central part of the lake in the summer (June to August) of 1999. It has been shown that the allochthonous microflora, which is brought into the lake with the resort effluent in mid-summer, is the anthropogenic factor contributing to an increase in the number of freshwater bacteria that feature multiple antibiotic resistance in the central part of the lake. It has been found that resistance to ampicillin of freshwater and halophile heterotrophic bacteria is related to the increase in the biomass of blue-green and green algae in the central part of Shira lake between mid-July and the end of August. A mathematical model has been constructed to describe the dynamics of the antibiotic resistance of heterotrophic bacteria in the close-to-resort and the central parts of Shira lake under natural and anthropogenic impacts.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Ctr Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Lobova, T.I.; Barkhatov, Y.V.; Popova, L.Y.

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14.

Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment [Text] / A. A. Tikhomirov [et al.] ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1711-1720, DOI 10.1016/S0273-1177(03)00108-X. - Cited References: 13 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system, The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Krasnoyarsk, Russia
Univ Strasbourg 1, Clermont Ferrand, France
ESA, Estec, Environm Control & Life Support Sect, Noordwijk, Netherlands
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Y.A.; Kovalev, V.S.; Gubanov, V.G.; Barkhatov, Y.V.; Gribovskaya, I.V.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, C...; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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15.

High temperature effect on microflora of radish root-inhabited zone and nutrient solutions for radish growth [Text] / E. V. Borodina, L. S. Tirranen ; ed.: G Horneck, ME Vazquez, Vazquez, ME // SPACE LIFE SCIENCES: MISSIONS TO MARS, RADIATION BIOLOGY, AND PLANTS AS A FOUNDATION FOR LONG-TERM LIFE SUPPORT SYSTEMS IN SPACE. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: F0 1 and F1 3-F2 3 Symposia of COSPAR Scientific Commission F held at the 33rd COSPAR Scientific Assembly (JUL, 2000, WARSAW, POLAND), Is. 1. - P. 235-240, DOI 10.1016/S0273-1177(02)00741-X. - Cited References: 7 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences

Аннотация: The effect of high temperatures (35 and 45 degreesC) on microflora of the root zone of radish plants grown in phytotron was evaluated by the response of microorganisms from 9 indicator groups. Phytotron air temperature elevated to 35 degreesC for 20 hours caused no significant changes in qualitative and quantitative composition of the root microflora in experimental plants. By the end of the experiment, the species diversity of microflora had changed. The amount of phytopathogenic microorganisms decreased which can be interpreted as more stable co-existence of microflora with plants. The numbers of microbes from other indicator groups was in dynamic equilibrium. The plants' condition did not deteriorate either. Exposure to the temperature of 45 degreesC for 7 hours have been found to change the numbers and species diversity in the radish root zone microflora. The microorganisms were observed to increase their total numbers at the expense of certain indicator groups. Bacteria increased spore forms at the stage of spores. Colon bacillus bacteria of increased their numbers by the end,of experiment by an order. By the end of experiment the roots of experiment plants had microscopic fungi from. Mucor, Aspergillus, Trichoderma, Cladosporium genera. The observed changes in the microbial complex seem to be associated with the changes of root emissions and general deterioration of the plants' condition. It is suggested that the response of the microorganisms can be indicative of the condition of plants under investigation. (C) 2002 Published by Elsevier Science Ltd on behalf of COSPAR.

WOS
Держатели документа:
Russian Acad Sci, Siberian Branch, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Borodina, E.V.; Tirranen, L.S.; Horneck, G \ed.\; Vazquez, ME \ed.\

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16.

Light intensity and production parameters of phytocenoses cultivated on soil-like substrate under controled environment conditions [Text] / A. A. Tikhomirov [et al.] ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1775-1780, DOI 10.1016/S0273-1177(03)00120-0. - Cited References: 11 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
SYSTEM
Аннотация: To increase the degree of closure of biological life support systems of a new generation, we used vermicomposting to involve inedible phytomass in the intra-system mass exchange. The resulting product was a soil-like substrate, which was quite suitable for growing plants (Manukovsky et al. 1996, 1997). However, the soil-like substrate can be regarded as a candidate for inclusion in a system only after a comprehensive examination of its physical, chemical, and other characteristics. An important criterion is the ability of the soil-like substrate to supply the necessary mineral elements to the photosynthesizing component under the chosen cultivation conditions. Thus, the purpose of this work was to study the feasibility of enhancing the production activity of wheat and radish crops by varying the intensity of photosynthetically active radiation, without decreasing the harvest index. The increase of light intensity from 920 to 1150 mumol(.)m(-2.)s(-1) decreased the intensity of apparent photosynthesis of the wheat crops and slightly increased the apparent photosynthesis of the radish crops The maximum total and grain productivity (kg/m(2)) of the wheat crops was attained at the irradiance of 920 mumol(.)m(-2.)s(-1). Light intensity of 1150 mumol(.)m(2.)s(-1) decreased the productivity of wheat plants and had no significant effect on the productivity of the radish crops (kg/m(2)) as compared to 920 mumol(.)m(-2.)s(-2). The qualitative and quantitative composition of microflora of the watering solution and substrate was determined by the condition of plants, developmental phase and light intensity. By the end of wheat growth under 1150 mumol(.)m(-2.)s(-1) the numbers of bacteria of the coliform family and phytopathogenic bacteria in the watering solution and substrate were an order of magnitude larger than under other illumination conditions. The obtained data suggest that the cultivation of plants in a life support system on soil-like substrate from composts has a number of advantages over the cultivation on neutral substrates, which require continual replenishment of the plant nutrient solution from the system's store to complement the macro- and micro- elements. Yet, a number of problems arise, including those related to the controlling of the production activity of the plants by the intensity of photosynthetically active radiation. It is essential to understand why the intensity of production processes is limited at higher irradiation levels and to overcome the factors responsible for this, so that the soil-like substrate could have an even better chance in the competition for the best plant cultivation technology to be used in biological life support systems. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
RAS SB, Inst Biophys, Krasnoyarsk 660036, Russia
Tomsk VV Kuibyshev State Univ, Tomsk 634050, Russia
Univ Clermont Ferrand, LGCB, F-63174 Aubiere, France
European Space Agcy, European Space Technol Ctr, NL-2200 AG Noordwijk, Netherlands
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Gribovskaya, I.A.; Tirranen, L.S.; Manukovsky, N.S.; Zolotukhin, I.G.; Karnachuk, R.A.; Gros, J.B.; Lasseur, C...; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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17.

Experimental evaluation of the processes resulting from the introduction of the transgenic microorganism Escherichia coli Z905/pPHL7 (luk(+)) into aquatic microcosms [Text] / T. V. Kargatova [et al.] ; ed. M Nelson [et al.] // SPACE LIFE SCIENCES: CLOSED ARTIFICIAL ECOSYSTEMS AND LIFE SUPPORT SYSTEMS. Ser. ADVANCES IN SPACE RESEARCH : PERGAMON-ELSEVIER SCIENCE LTD, 2003. - Vol. 31: Meeting of F4 1 Session of the 34th Scientific Assembly of COSPAR (OCT, 2002, HOUSTON, TEXAS), Is. 7. - P. 1769-1774, DOI 10.1016/S0273-1177(03)00119-4. - Cited References: 16 . - ISBN 0273-1177

РУБ Engineering, Aerospace + Astronomy & Astrophysics + Ecology + Geosciences, Multidisciplinary + Meteorology & Atmospheric Sciences
Рубрики:
SURVIVAL
PROTEIN
Аннотация: The processes resulting from the introduction of the transgenic microorganism (TM) E. coli Z905/pPHL7 into aquatic microcosms have been modeled experimentally. It has been shown that the TM E. coli is able to adapt to a long co-existence with indigenous heterotrophic microflora in variously structured microcosms. In more complex microcosms the numerical dynamics of the introduced E. coli Z905/pPHL7 population is more stable. In the TM populations staying in the microcosms for a prolonged time, changes are recorded in the phenotypic expression of plasmid genes (ampicillin resistance and the luminescence level) and chromosome genes (morphological and physiological traits). However, in our study microcosms, the recombinant plasmid persisted in the TM cells for 6 years after die introduction, and as the population adapts to the conditions of the microcosms, the efficiency of the cloned gene expression in the cells is restored. In the microcosms with high microalgal counts (10(7) cells/ml), cells with a high threshold of sensitivity to ampicillin dominate in the population of the TM E. coli Z905/pPHL7. (C) 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
SB RAS, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Kargatova, T.V.; Boyandin, A.N.; Popova, L.Y.; Pechurkin, N.S.; Nelson, M \ed.\; Pechurkin, NS \ed.\; Dempster, WF \ed.\; Somova, LA \ed.\; Somo, , LA \ed.\

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18.

Psychrophilic and psychrotolerant heterotrophic microorganisms of Middle Siberian karst cavities [Text] / S. V. Khizhnyak [et al.] // Russ. J. Ecol. - 2003. - Vol. 34, Is. 4. - P. 231-235, DOI 10.1023/A:1024537513439. - Cited References: 12 . - ISSN 1067-4136

РУБ Ecology

Кл.слова (ненормированные):
psychrophilic and psychrotolerant microorganisms -- karst cavities -- caves -- heterotrophic microorganisms
Аннотация: The natural microflora of Middle Siberian karst cavities, which comprises psychrotolerant bacteria and fungi capable of growing at 3-15 and 3...+28degreesC, respectively, has been studied. Bacteria are ubiquitous in caves, their count varying from 10(3) to 10(7) cells/g ground. The bacteria have been identified as Pseudomonas, Arthrobacter, Bacillus, and coryneform bacteria. Fungi have been found in places exposed to increased anthropogenic impact, their count being as large as 10(6) to 10(7) cells/g ground. Mucor Penicillium, and Chrysosporium were dominant fungal genera.

WOS
Держатели документа:
Krasnoyarsk State Agrarian Univ, Krasnoyarsk 660001, Russia
Russian Acad Sci, Siberian Div, Inst Biophys, Krasnoyarsk 660036, Russia
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Khizhnyak, S.V.; Tausheva, I.V.; Berezikova, A.A.; Nesterenko, E.V.; Rogozin, D.Y.

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19.

Synthesis of biomass and utilization of plants wastes in a physical model of biological life-support system [Text] / A. A. Tikhomirov [et al.] // Acta Astronaut. - 2003. - Vol. 53: 53rd Congress of the International-Astronautical-Federation (IAF) (OCT 10, 2002, HOUSTON, TEXAS), Is. 04.10.2013. - P. 249-257, DOI 10.1016/S0094-5765(03)00137-1. - Cited References: 16 . - ISSN 0094-5765

РУБ Engineering, Aerospace

Аннотация: The paper considers problems of biosynthesis of higher plants' biomass and "bioloaical incineration" of plant wastes in a working physical model of biological LSS. The plant wastes are "biologically incinerated" in a special heterotrophic block involving Californian worms, mushrooms and straw. The block processes plant wastes (straw, haulms) to produce soil-like substrate (SLS) on which plants (wheat, radish) are grown. Gas exchange in such a system consists of respiratory gas exchange of SLS and photosynthesis and respiration of plants. Specifics of gas exchange dynamics of high plants - SLS complex has been considered. Relationship between such a gas exchange and PAR irradiance and age of plants has been established. Nitrogen and iron were found to the first to limit plants' growth on SLS when process conditions are deranged. The SLS microflora has been found to have different kinds of ammonifying and denitrifying bacteria which is indicative of intensive transformation of nitrogen-containing compounds. The number of physiological groups of microorganisms in SLS was, on the whole, steady. As a result, organic substances - products of exchange of plants and microorganisms were not accumulated in the medium, but mineralized and assimilated by the biocenosis. Experiments showed that the developed model of a manmade ecosystem realized complete utilization of plant wastes and involved them into the intrasystem turnover. (C) 2003 International Astronautical Federation. Published by Elsevier Science Ltd. All rights reserved.

WOS
Держатели документа:
Russian Acad Sci, Inst Biophys, Siberian Branch, Krasnoyarsk, Russia
Univ B Pascal, Clermont Ferrand, France
Estec, ESA, Environm Control & Life Support Sect, Noordwijk, Netherlands
ИБФ СО РАН : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Y.A.; Kovalev, V.S.; Gribovskaya, I.V.; Tirranen, L.S.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, C...

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20.

Mass exchange in an experimental new-generation life support system model based on biological regeneration of environment / A. A. Tikhomirov [et al.] // Advances in Space Research. - 2003. - Vol. 31, Is. 7. - P1711-1720, DOI 10.1016/S0273-1177(03)80017-0 . - ISSN 0273-1177
Кл.слова (ненормированные):
Biomass -- Photosynthesis -- Plants (botany) -- Transpiration -- Mass exchange -- Life support systems (spacecraft) -- ammonia -- nitrogen -- oxygen -- biosphere -- animal -- annelid worm -- article -- biological model -- biomass -- bioremediation -- evaluation -- growth, development and aging -- human -- metabolism -- microclimate -- photosynthesis -- Pleurotus -- radish -- wheat -- Ammonia -- Animals -- Biodegradation, Environmental -- Biomass -- Ecological Systems, Closed -- Evaluation Studies -- Humans -- Life Support Systems -- Models, Biological -- Nitrogen -- Oligochaeta -- Oxygen -- Photosynthesis -- Pleurotus -- Raphanus -- Triticum
Аннотация: An experimental model of a biological life support system was used to evaluate qualitative and quantitative parameters of the internal mass exchange. The photosynthesizing unit included the higher plant component (wheat and radish), and the heterotrophic unit consisted of a soil-like substrate, California worms, mushrooms and microbial microflora. The gas mass exchange involved evolution of oxygen by the photosynthesizing component and its uptake by the heterotroph component along with the formation and maintaining of the SLS structure, growth of mushrooms and California worms, human respiration, and some other processes. Human presence in the system in the form of "virtual human" that at regular intervals took part in the respirative gas exchange during the experiment. Experimental data demonstrated good oxygen/carbon dioxide balance, and the closure of the cycles of these gases was almost complete. The water cycle was nearly 100% closed. The main components in the water mass exchange were transpiration water and the watering solution with mineral elements. Human consumption of the edible plant biomass (grains and roots) was simulated by processing these products by a unique physicochemical method of oxidizing them to inorganic mineral compounds, which were then returned into the system and fully assimilated by the plants. The oxidation was achieved by "wet combustion" of organic biomass, using hydrogen peroxide following a special procedure, which does not require high temperature and pressure. Hydrogen peroxide is produced from the water inside the system. The closure of the cycle was estimated for individual elements and compounds. Stoichiometric proportions are given for the main components included in the experimental model of the system. Approaches to the mathematical modeling of the cycling processes are discussed, using the data of the experimental model. Nitrogen, as a representative of biogenic elements, shows an almost 100% closure of the cycle inside the system. The proposed experimental model of a biological system is discussed as a candidate for potential application in the investigations aimed at creating ecosystems with largely closed cycles of the internal mass exchange. The formation and maintenance of sustainable cycling of vitally important chemical elements and compounds in biological life support systems (BLSS) is an extremely pressing problem. To attain the stable functioning of biological life support systems (BLSS) and to maintain a high degree of closure of material cycles in them, it is essential to understand the character of mass exchange processes and stoichiometric proportions of the initial and synthesized components of the system. В© 2003 COSPAR. Published by Elsevier Science Ltd. All rights reserved.

Scopus
Держатели документа:
Institute of Biophysics, Russian Academy of Sciences, Siberian Branch, Krasnoyarsk, Russian Federation
Universite B. Pascal, Clermont-Ferrand, France
Environ. Control/Life Support Sect., ESA, Estec Noorwijk, Netherlands : 660036, Красноярск, Академгородок, д. 50, стр. 50

Доп.точки доступа:
Tikhomirov, A.A.; Ushakova, S.A.; Manukovsky, N.S.; Lisovsky, G.M.; Kudenko, Yu.A.; Kovalev, V.S.; Gubanov, V.G.; Barkhatov, Yu.V.; Gribovskaya, I.V.; Zolotukhin, I.G.; Gros, J.B.; Lasseur, Ch.

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