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Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Esimbekova, Elena, Kratasyuk, Valentina, Shimomura, Osamu
Заглавие : Application of Enzyme Bioluminescence in Ecology
Колич.характеристики :43 с
Место публикации : Adv. Biochem. Eng. Biotechnol.: SPRINGER-VERLAG BERLIN, 2014. - Vol. 144. - С. 67-109. - (Advances in Biochemical Engineering-Biotechnology). - , DOI 10.1007/978-3-662-43385-0_3
Примечания : Cited References:85
Предметные рубрики: BACTERIAL LUCIFERASE
IN-VITRO
PYRETHROID INSECTICIDES
FRESH-WATER
Ключевые слова (''Своб.индексиров.''): bioluminescence--ecological monitoring--enzymatic assay--immobilization--integral water toxicity--luciferase
Аннотация: This review examines the general principles of bioluminescent enzymatic toxicity bioassays and describes the applications of these methods and the implementation in commercial biosensors. Bioluminescent enzyme system technology (BEST) has been proposed in the bacterial coupled enzyme system, wherein NADH: FMN-oxidoreductase-luciferase substitutes for living organisms. BEST was introduced to facilitate and accelerate the development of cost-competitive enzymatic systems for use in biosensors for medical, environmental, and industrial applications. For widespread use of BEST, the multicomponent reagent "Enzymolum'' has been developed, which contains the bacterial luciferase, NADH: FMN-oxidoreductase, and their substrates, co-immobilized in starch or gelatin gel. Enzymolum is the central part of Portable Laboratory for Toxicity Detection (PLTD), which consists of a biodetector module, a sampling module, a sample preparation module, and a reagent module. PLTD instantly signals chemical-biological hazards and allows us to detect a wide range of toxic substances. Enzymolum can be integrated as a biological module into the portable biodetector-biosensor originally constructed for personal use. Based on the example of Enzymolum and the algorithm for creating new enzyme biotests with tailored characteristics, a new approach was demonstrated in biotechnological design and construction. The examples of biotechnological design of various bioluminescent methods for ecological monitoring were provided. Possible applications of enzyme bioassays are seen in the examples for medical diagnostics, assessment of the effect of physical load on sportsmen, analysis of food additives, and in practical courses for higher educational institutions and schools. The advantages of enzymatic assays are their rapidity (the period of time required does not exceed 3-5 min), high sensitivity, simplicity and safety of procedure, and possibility of automation of ecological monitoring; the required luminometer is easily available.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lonshakova-Mukina V..., Esimbekova E..., Kratasyuk V...
Заглавие : Biological module of bioluminescent biosensor for environmental toxicity assay
Колич.характеристики :1 с
Место публикации : Luminescence: WILEY-BLACKWELL, 2014. - Vol. 29. - С. 72-72. - ISSN 1522-7235. - ISSN 1522-7243
Примечания : Cited References: 4
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Lonshakova-Mukina V., Esimbekova E., Kratasyuk V.
Заглавие : Impact of enzyme stabilizers on the characteristics of biomodules for bioluminescent biosensors
Место публикации : Sens Actuators, B Chem. - 2015. - Vol. 213. - С. 244-247. - ISSN 09254005 (ISSN) , DOI 10.1016/j.snb.2015.02.061
Ключевые слова (''Своб.индексиров.''): bioluminescence--body fluids--carrier concentration--enzymes--starch--bioluminescent biosensor--bovine serum albumins--dithiothreitol--maximum permissible concentration--mercaptoethanol--oxidoreductases--storage stability--toxic substances--biosensors
Аннотация: The biomodule of bioluminescent biosensor based on a coupled enzyme system NADH:FMN-oxidoreductase and luciferase, co-immobilized with substrates in dried starch or gelatin gels, has been developed. We studied the impact of several stabilizers - dithiothreitol (DTT), bovine serum albumin (BSA) and mercaptoethanol (ME) on the biomodule's activity, storage stability and sensitivity to toxic substances. The inclusion of stabilizers increases the activity of the biological module by more than 150%. To achieve the combination of high activity, prolonged storage time and acute sensitivity to toxic substances within maximum permissible concentration we used starch gel as a carrier adding 100 ?M DTT to the immobilized preparation. The gelatin-based biological module had greater storage stability than the starch-based one but demonstrated less sensitivity to toxic substances. © 2015 Elsevier B.V. All rights reserved.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Kovalev V. S., Grandl W., Manukovsky N. S., Tikhomirov A. A., Bock C.
Заглавие : Modeling a lunar base mushroom farm
Колич.характеристики :6 с
Место публикации : Life Sci. Space Res.: ELSEVIER, 2022. - Vol. 33. - С. 1-6. - ISSN 2214-5524, DOI 10.1016/j.lssr.2021.12.005. - ISSN 2214-5532(eISSN)
Примечания : Cited References:31
Предметные рубрики: SUPPORT
CULTIVATION
Аннотация: To calculate the equivalent system mass of mushrooms, a conceptual configuration of a mushroom farm as part of a bioregenerative life support system on an inhabited lunar base was designed. The mushroom farm consists of two connected modules. Each module is a double-shell rigid pipe-in-pipe aluminum structure. The first module is used to prepare and sterilize the substrate, while the mushrooms are sown and grown in the second module. Planned productivity of the mushroom farm is 28 kg of fresh mushrooms per one process cycle lasting 66 days for 14 consumers. Mushroom production can be increased using additional modules. The calculated equivalent system masses of the mushroom farm and the mushrooms produced therein is 88,432 kg and 31,550 kg per 1 kg of dry mushrooms in one process cycle, respectively. At that, the biggest contributor to the equivalent system mass of mushrooms is the total pressurized volume of the farm - 68%. The results obtained may be a prerequisite for performing trade-off studies between different configurations of mushroom farm and calculating a space diet using the equivalent system mass of mushrooms.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Davydova, Anna, Krasitskaya, Vasilisa, Vorobjev, Pavel, Timoshenko, Valentina, Tupikin, Alexey, Kabilov, Marsel, Frank, Ludmila, Venyaminova, Alya, Vorobyeva, Mariya
Заглавие : Reporter-recruiting bifunctional aptasensor for bioluminescent analytical assays
Колич.характеристики :7 с
Коллективы : Russian Science FoundationRussian Science Foundation (RSF) [16-14-10296]; Russian State funded budget projects [AAAA-A17-117020210021-7, AAAA-A19-119031890015-0]
Место публикации : RSC Adv.: ROYAL SOC CHEMISTRY, 2020. - Vol. 10, Is. 54. - С. 32393-32399. - ISSN 2046-2069(eISSN), DOI 10.1039/d0ra05117a
Примечания : Cited References:33. - The work was supported by the Russian Science Foundation (grant #16-14-10296), Russian State funded budget projects #AAAA-A17-117020210021-7 to ICBFM SB RAS and #AAAA-A19-119031890015-0 to IBP SB RAS.
Предметные рубрики: DNA APTAMER
RNA APTAMER
OBELIN
PURIFICATION
EXPRESSION
SEQUENCES
Аннотация: We report a novel bioluminescent aptasensor, which consists of 2 '-F-RNA aptamer modules joined into a bi-specific aptamer construct. One aptamer module binds the analyte, then after structural rearrangement the second module recruits non-covalently Ca2+-dependent photoprotein obelin from the solution, thus providing a bioluminescent signal. This concept allows using free protein as a reporter, which brings such advantages as no need for aptamer-protein conjugation, a possibility of thermal re-folding of aptamer component with no harm to a protein, and simpler detection protocol. We developed the new 2 '-F-RNA aptamer for obelin, and proposed the strategy for engineering structure-switching bi-modular aptamer constructs which bind the analyte and the obelin in a sequential manner. With the use of hemoglobin as a model analyte, we showed the feasibility of utilizing the aptasensor in a fast and straightforward bioluminescent microplate assay. With a proper design of a secondary structure, this strategy of aptasensor engineering might be further extended to bi-specific aptamer-based bioluminescent sensors for other analytes of interest.
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Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Bartsev S.I., Okhonin V.A.
Заглавие : Self-restoration of biocomponents as a mean to enhance biological life support systems reliability
Колич.характеристики :4 с
Место публикации : LIFE SCIENCES: ARTIFICIAL ECOSYSTEMS. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1999. - Vol. 24: F4 3 and F4 4 Symposia of COSPAR Scientific Commission F Held at 31st COSPAR Scientific Assembly (JUL 14-21, 1996, BIRMINGHAM, ENGLAND), Is. 3. - P393-396. - ISBN 0273-1177, DOI 10.1016/S0273-1177(99)00489-5
Примечания : Cited References: 5
Аннотация: One of the key problems of long-term space missions is limited service life of units. The only exceptions are biological components of biological Life Support Systems - higher plants or microorganisms. These components are capable of self-restoration: after complete disintegration, they can appear again from seeds or spores. The estimate of failure intensity of BLSS regeneration component includes: a number of self-sustained sections of the regeneration component; permissible boost (how many times can productivity of a component be increased); time required to repair (restore) a component; the crew existence time, when all LSS regeneration components fail; failure rate of one section of a regeneration component. Evaluations show that for hydrogen-oxidizing bacteria and micro-algae very high reliability is achieved even for one or two sections. In the case of higher plants (due to low rate of self-restoration) bio-regenerative module has to be divided into 10 self-sustained sections operating simultaneously. These measures can decrease the probability of catastrophe by a factor of 10 (C) 1999 COSPAR. Published by Elsevier Science Ltd.
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Вид документа : Статья из журнала
Шифр издания :
Автор(ы) : Gurevich Y.L., Manukovsky N.S., Kovalev V.S., Degermendzy A.G., Hu D.W., Hue E.Z., Liu H...
Заглавие : The carbon cycle in a bioregenerative life support system with a soil-like substrate
Место публикации : Acta Astronaut.: PERGAMON-ELSEVIER SCIENCE LTD, 2008. - Vol. 63: 16th IAA Humans in Space Symposium (MAY 20-24, 2007, Beijing, PEOPLES R CHINA), Is. 07.10.2013. - С. 1043-1048. - 6. - ISSN 0094-5765, DOI 10.1016/j.actaastro.2008.03.009
Примечания : Cited References: 28
Предметные рубрики: ECOSYSTEM
PLANTS
MODEL
Ключевые слова (''Своб.индексиров.''): life support system--soil-like substrate--carbon cycle--mass-flow model
Аннотация: A mass-flow model of carbon cycle in a bioregenerative life support system (BLSS) including Resource, Plant Growth, Food Processing, Human, Waste Processing, and Waste Storage Modules was developed. A human received food from Plant Growth and Resource Modules. Plants were assumed to be growing on the soil-like substrate (SLS). Another function of SLS was balancing the carbon cycle. The input of BLSS was set to 81 g of carbon per day along with food from the Resource Module. To balance the carbon cycle an equal arnount of carbon was removed from BLSS along with urine, feces, plant wastes, and SLS. A mass flow of carbon cycle was used to simulate the effect of light intensity on the basic parameters of the Plant Growth Module. It was calculated that the stationary dry mass of SLS increases from 10 to 35 kg m(-2) with increase canopy-level daily photosynthetic photon flux (PPF) from 34 to 178 mol m(-2) d(-1). On the contrary dry mass of SLS needed to provide one person with plant food is not dependent on light intensity. (C) 2008 Elsevier Ltd. All rights reserved.
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Вид документа : Статья из сборника (выпуск монографической серии)
Шифр издания :
Автор(ы) : Belobrov, P., I, Evstrapov A. A., Esimbekova E. N., Denisov I. A., Lukyanenko K. A., Osipova E. D., Yakimov A. S.
Заглавие : Towards biological quantity theory for nominal property metrology in polyenzymatic devices with living cells
Колич.характеристики :6 с
Место публикации : JOINT IMEKO TC1-TC7-TC13-TC18 SYMPOSIUM: IOP PUBLISHING LTD, 2019. - Vol. 1379: Joint IMEKO TC1-TC7-TC13-TC18 Symposium (JUL 02-05, 2019, St Petersburg, RUSSIA). - Ст.012036. - (Journal of Physics Conference Series). - , DOI 10.1088/1742-6596/1379/1/012036
Примечания : Cited References:19
Аннотация: Here we discuss the concepts of "biological quantity" and "nominal property" within the framework of the problem of biological measurements based on new specific results of biological analysis using a microfluidic platform and chips developed by our team earlier. It was shown that based on different microfluidic platforms it is possible to develop chips with a polyenzymatic bioluminescent system NAD(P)H:FMN-oxidoreductase-luciferase (Red + Luc), which can be used in various areas of biological analysis. Thus, disposable microfluidic chips with Red + Luc system suitable for field and indoor use were developed using continuous flow microfluidic platform. The use of droplet-based microfluidic platform allowed to develop microfluidic chips with Red + Luc system for long-term continuous measurements of water samples, for example, in places of waste disposal by industrial enterprises. The reference for comparing different biological quantities with each other in the proposed system was a photodetector, which converted non-numeric values and nominal properties recorded by a biological module Red + Luc into numerical variables. Such a reference was implemented as a portable luminometer based on silicon photomultiplier. The results allow to perform other biological measurements and to start the discussion of modern biological concepts in the language of biological measures.
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Вид документа : Статья из сборника (выпуск продолж. издания)
Шифр издания :
Автор(ы) : Belobrov P. I., Evstrapov A. A., Esimbekova E. N., Denisov I. A., Lukyanenko K. A., Osipova E. D., Yakimov A. S.
Заглавие : Towards biological quantity theory for nominal property metrology in polyenzymatic devices with living cells
Место публикации : Journal of Physics: Conference Series: Institute of Physics Publishing, 2019. - Vol. 1379: Joint IMEKO TC1-TC7-TC13-TC18 Symposium 2019 (2 July 2019 through 5 July 2019, ) Conference code: 156337, Is. 1. - Ст.012036. - , DOI 10.1088/1742-6596/1379/1/012036
Аннотация: Here we discuss the concepts of "biological quantity" and "nominal property" within the framework of the problem of biological measurements based on new specific results of biological analysis using a microfluidic platform and chips developed by our team earlier. It was shown that based on different microfluidic platforms it is possible to develop chips with a polyenzymatic bioluminescent system NAD(P)H:FMN-oxidoreductase-luciferase (Red + Luc), which can be used in various areas of biological analysis. Thus, disposable microfluidic chips with Red + Luc system suitable for field and indoor use were developed using continuous flow microfluidic platform. The use of droplet-based microfluidic platform allowed to develop microfluidic chips with Red + Luc system for long-term continuous measurements of water samples, for example, in places of waste disposal by industrial enterprises. The reference for comparing different biological quantities with each other in the proposed system was a photodetector, which converted non-numeric values and nominal properties recorded by a biological module Red + Luc into numerical variables. Such a reference was implemented as a portable luminometer based on silicon photomultiplier. The results allow to perform other biological measurements and to start the discussion of modern biological concepts in the language of biological measures. © 2019 IOP Publishing Ltd. All rights reserved.
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10.

Вид документа : Статья из сборника (однотомник)
Шифр издания :
Автор(ы) : Manukovsky N.S., Kovalev V.S., Rygalov V.Y., Zolotukhin I.G.
Заглавие : Waste bioregeneration in life support CES: Development of soil organic substrate
Колич.характеристики :6 с
Место публикации : LIFE SCIENCES: LIFE SUPPORT SYSTEMS STUDIES-I. Ser. ADVANCES IN SPACE RESEARCH: PERGAMON PRESS LTD, 1997. - Vol. 20: F4.6, F4.8, F4.2 and F4.9 Symposia of COSPAR Scientific Commission F on Life Sciences - Life Support System Studies-I, at the 31st COSPAR Scientific Assembly (JUL 14-SEP 21, 1996, BIRMINGHAM, ENGLAND), Is. 10. - P1827-1832. - ISBN 0273-1177, DOI 10.1016/S0273-1177(97)00848-X. - ISBN 0-08-043307-3
Примечания : Cited References: 13
Аннотация: An experimental model of matter turnover in the biotic cycle: plants (plant biomass) -- mushrooms (residual substrate + mushroom fruit bodies) -- worms (biohumus) -- microorganisms (soil-like substrate) -- plants is presented. The initial mass of soillike substrate was produced from wheat plants grown in a hydroponic system, Three cycles of matter turnover in the biotic cycle were carried out. Grain productivity on soillike substrate was 21.87 g/m(2) day(1). The results obtained were used for designing a CES containing man, plants, soillike substrate, bioregeneration module and aquaculture. It was shown, that the circulating dry mass of the CES is 756 kg. The main part (88%) of the circulating mass accumulates in the soillike substrate and bioregeneration module. (C) 1997 COSPAR. Published by Elsevier Science Ltd.
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